##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211303_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4627075 Sequences flagged as poor quality 0 Sequence length 100 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11080131616626 33.0 31.0 34.0 30.0 34.0 2 32.234148355062324 33.0 31.0 34.0 30.0 34.0 3 32.29661308710146 34.0 31.0 34.0 30.0 34.0 4 35.94750981991863 37.0 35.0 37.0 35.0 37.0 5 35.864943836008706 37.0 35.0 37.0 35.0 37.0 6 35.889059935272286 37.0 35.0 37.0 35.0 37.0 7 35.82218896387026 37.0 35.0 37.0 35.0 37.0 8 35.80335071292339 37.0 35.0 37.0 35.0 37.0 9 37.42425463170578 39.0 37.0 39.0 34.0 39.0 10-11 37.36864142033574 39.0 37.0 39.0 34.0 39.0 12-13 37.33073539979361 39.0 37.0 39.0 34.0 39.0 14-15 38.55561288719115 40.0 38.0 41.0 34.0 41.0 16-17 38.54234921629755 40.0 38.0 41.0 34.0 41.0 18-19 38.594935029149084 40.0 38.0 41.0 34.0 41.0 20-21 38.577669910256475 40.0 38.0 41.0 34.0 41.0 22-23 38.44484712264227 40.0 38.0 41.0 34.0 41.0 24-25 38.36419606338778 40.0 38.0 41.0 34.0 41.0 26-27 38.27316695752717 40.0 38.0 41.0 34.0 41.0 28-29 38.18386215913941 40.0 38.0 41.0 33.5 41.0 30-31 38.16531005008564 40.0 38.0 41.0 34.0 41.0 32-33 38.07178573937099 40.0 37.0 41.0 33.5 41.0 34-35 37.93076219858118 40.0 37.0 41.0 33.0 41.0 36-37 37.76469875677399 40.0 37.0 41.0 33.0 41.0 38-39 37.6591482740176 40.0 37.0 41.0 33.0 41.0 40-41 37.57610401819724 40.0 37.0 41.0 33.0 41.0 42-43 37.49177482534863 40.0 37.0 41.0 32.5 41.0 44-45 37.36025361594528 40.0 37.0 41.0 32.0 41.0 46-47 37.133742483102175 40.0 36.0 41.0 31.0 41.0 48-49 35.60924568977162 39.0 35.0 41.0 24.0 41.0 50-51 36.39476926568081 39.0 35.0 41.0 29.0 41.0 52-53 36.537327253178304 39.0 36.0 40.5 30.0 41.0 54-55 36.40819880810231 39.0 35.0 40.0 29.5 41.0 56-57 36.24781368359061 39.0 35.0 40.0 29.0 41.0 58-59 36.07282916313221 39.0 35.0 40.0 28.5 41.0 60-61 35.863527930712166 38.0 35.0 40.0 28.0 41.0 62-63 35.5843861402722 38.0 34.5 40.0 28.0 41.0 64-65 35.29307164893588 38.0 34.0 40.0 28.0 41.0 66-67 34.981028619592294 37.0 34.0 40.0 27.0 41.0 68-69 34.618472469108454 37.0 34.0 39.0 26.0 40.5 70-71 34.26281679030489 36.0 33.5 39.0 26.0 40.0 72-73 33.86657337518843 36.0 33.0 38.5 26.0 40.0 74-75 33.46223197160193 35.0 32.5 38.0 26.0 39.5 76-77 32.450993122004725 34.5 31.5 36.5 24.5 39.0 78-79 32.850639658963814 35.0 32.0 37.0 26.0 39.0 80-81 32.66992408811182 35.0 32.0 36.0 25.5 38.5 82-83 32.34798279690733 35.0 32.0 36.0 25.0 37.0 84-85 31.997648946688784 34.5 31.5 35.5 24.5 37.0 86-87 31.723235953599197 34.0 31.0 35.0 24.0 36.5 88-89 31.454289156756698 34.0 31.0 35.0 23.5 36.0 90-91 31.20984315145097 34.0 31.0 35.0 23.0 36.0 92-93 31.004795145961545 34.0 31.0 35.0 21.5 35.5 94-95 30.792226730710006 34.0 30.0 35.0 20.0 35.0 96-97 30.59611179849041 34.0 30.0 35.0 20.0 35.0 98-99 30.385056974438495 34.0 30.0 35.0 19.5 35.0 100 27.986306467908992 31.0 25.0 34.0 12.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.24328316683430629 1101 2 -0.24553035780022014 1101 3 -0.2830045163725039 1101 4 -0.12458430157581546 1101 5 -0.13765322250450396 1101 6 -0.20647461962580138 1101 7 -0.20373124848462254 1101 8 -0.2293883113919719 1101 9 -0.3088900794002427 1101 10-11 -0.33732416785788644 1101 12-13 -0.3190574350126312 1101 14-15 -0.4352732930748715 1101 16-17 -0.4173820050371688 1101 18-19 -0.4650169761909808 1101 20-21 -0.5474023306115043 1101 22-23 -0.5799585695252176 1101 24-25 -0.5299869068623124 1101 26-27 -0.610531452770644 1101 28-29 -0.6085657268035192 1101 30-31 -0.4206341528512709 1101 32-33 -0.4072885140635165 1101 34-35 -0.47718941286505867 1101 36-37 -0.48144943999773204 1101 38-39 -0.5287321990910456 1101 40-41 -0.4941507631157265 1101 42-43 -0.48152297926645815 1101 44-45 -0.5211925317902271 1101 46-47 -0.44035156724768143 1101 48-49 0.7558057435356815 1101 50-51 -0.09043392126463345 1101 52-53 -0.37965346098297204 1101 54-55 -0.4028231950787031 1101 56-57 -0.3464849007775186 1101 58-59 -0.4693721072442756 1101 60-61 -0.4363676504406371 1101 62-63 -0.5497365666940865 1101 64-65 -0.5914055851984514 1101 66-67 -0.4315833677159091 1101 68-69 -0.43974667453834115 1101 70-71 -0.3556346498027523 1101 72-73 -0.5013756329213805 1101 74-75 -0.5545390087753574 1101 76-77 -0.4923453929957944 1101 78-79 -0.4365938514562657 1101 80-81 -0.29155566526273446 1101 82-83 -0.31561394111738394 1101 84-85 -0.4595633032451616 1101 86-87 -0.5189946965829115 1101 88-89 -0.4742467112913573 1101 90-91 -0.4071555672137066 1101 92-93 -0.5603987094819338 1101 94-95 -0.5412748755144356 1101 96-97 -0.640247043477526 1101 98-99 -0.7816116371583952 1101 100 -0.5359763827963135 1102 1 -0.2674828816455239 1102 2 -0.2449230347494833 1102 3 -0.2665287698607095 1102 4 -0.14279851032033974 1102 5 -0.11043460602858346 1102 6 -0.18106025871311004 1102 7 -0.15163178752600714 1102 8 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-0.6191782701482396 1102 72-73 -0.625362742489628 1102 74-75 -0.7083047701792182 1102 76-77 -0.7247240622919477 1102 78-79 -0.5975673565664223 1102 80-81 -0.4385870997133807 1102 82-83 -0.4799298317671834 1102 84-85 -0.3801922959197519 1102 86-87 -0.39792358116243065 1102 88-89 -0.39674554198360923 1102 90-91 -0.39140713223037693 1102 92-93 -0.444331682948242 1102 94-95 -0.5498628030287414 1102 96-97 -0.5359477445560721 1102 98-99 -0.7465566554434595 1102 100 -0.6749639499656119 1103 1 -0.35183896185228036 1103 2 -0.33408444550358496 1103 3 -0.3675191141472638 1103 4 -0.19978546292549737 1103 5 -0.3009159453448689 1103 6 -0.22324640238868199 1103 7 -0.31630175740763633 1103 8 -0.35569465952586654 1103 9 -0.4243012592344826 1103 10-11 -0.45000443691376546 1103 12-13 -0.4974634748200728 1103 14-15 -0.5881135295250175 1103 16-17 -0.6076534704086285 1103 18-19 -0.6561091620210178 1103 20-21 -0.6027270738273955 1103 22-23 -0.5706672377752113 1103 24-25 -0.6564508352030103 1103 26-27 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1105 6 -0.29612586074532743 1105 7 -0.43606213221760015 1105 8 -0.35801546340056234 1105 9 -0.4128424278594096 1105 10-11 -0.3903947038358737 1105 12-13 -0.4509983419806787 1105 14-15 -0.5767002495421991 1105 16-17 -0.6754217912803213 1105 18-19 -0.623938123890099 1105 20-21 -0.6637691043117826 1105 22-23 -0.648097624962034 1105 24-25 -0.6108675117598636 1105 26-27 -0.6438564251635341 1105 28-29 -0.7001614976419788 1105 30-31 -0.5804680300822085 1105 32-33 -0.6293696898716874 1105 34-35 -0.48202510147143585 1105 36-37 -0.5683956646984925 1105 38-39 -0.5588807900737436 1105 40-41 -0.5058303082495286 1105 42-43 -0.577971985046851 1105 44-45 -0.5894516181295515 1105 46-47 -0.6528287286405074 1105 48-49 0.6190333963277439 1105 50-51 -0.15677414267436518 1105 52-53 -0.45122251948967573 1105 54-55 -0.5469011613332881 1105 56-57 -0.6655484851476032 1105 58-59 -0.669251901751359 1105 60-61 -0.7747363359058355 1105 62-63 -0.7291167416627644 1105 64-65 -0.7137949712658482 1105 66-67 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0.7759323345660079 2202 92-93 0.7799220600614483 2202 94-95 0.7427471083819093 2202 96-97 0.6949895487461504 2202 98-99 0.219160834427214 2202 100 0.5464065128023101 2203 1 0.5886299583928576 2203 2 0.5196735154664864 2203 3 0.5430450654404311 2203 4 0.3494972469573554 2203 5 0.3957533015831345 2203 6 0.4101706673733929 2203 7 0.48323353264265734 2203 8 0.45602353912871507 2203 9 0.5722200827568145 2203 10-11 0.5129532730911563 2203 12-13 0.5780460704567574 2203 14-15 0.7324806554598098 2203 16-17 0.6991537810647017 2203 18-19 0.6567503421857097 2203 20-21 0.6519996494547939 2203 22-23 0.712615645251816 2203 24-25 0.719394932774037 2203 26-27 0.7447554971106314 2203 28-29 0.601973775657278 2203 30-31 0.6755951390303565 2203 32-33 0.746122146402378 2203 34-35 0.7494839953182222 2203 36-37 0.7807907781880061 2203 38-39 0.8307536630136241 2203 40-41 0.9324507370610675 2203 42-43 0.8682831485487981 2203 44-45 0.8983182897507689 2203 46-47 0.9100896899444493 2203 48-49 2.153042801423993 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1.030438415762525 2204 76-77 1.126118569701056 2204 78-79 0.9212875015321487 2204 80-81 0.9135813506623478 2204 82-83 0.8535773812414149 2204 84-85 0.9000770218632823 2204 86-87 0.9236113883367914 2204 88-89 0.75288035086081 2204 90-91 0.10050966889041746 2204 92-93 0.6569938978630425 2204 94-95 0.9120122398189672 2204 96-97 1.00666697360613 2204 98-99 1.0619649985153998 2204 100 1.2847235689128347 2205 1 0.4489695880275093 2205 2 0.4712142184404655 2205 3 0.48472758962596885 2205 4 0.31865584173258554 2205 5 0.037815954225877135 2205 6 0.2790420188257343 2205 7 0.37283469155985927 2205 8 0.3889715460532912 2205 9 0.5826770806309227 2205 10-11 0.5831107032239089 2205 12-13 0.5828113113780446 2205 14-15 0.7214452536430827 2205 16-17 0.723870643370212 2205 18-19 0.6905210567759781 2205 20-21 0.6690060662883184 2205 22-23 0.6462480867974634 2205 24-25 0.7188645157175202 2205 26-27 0.6665702451305151 2205 28-29 0.7016009544652988 2205 30-31 0.7127861810765097 2205 32-33 0.7232969994660863 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1.1910470758046863 2206 1 0.5639805903343316 2206 2 0.5430331335443199 2206 3 0.5313393666370203 2206 4 0.31910668147561694 2206 5 0.3952817894594105 2206 6 0.3842870057404113 2206 7 0.4524152743164862 2206 8 0.47296435300435746 2206 9 0.5739956733106766 2206 10-11 0.574186868437998 2206 12-13 0.6335390369696583 2206 14-15 0.7482986382097181 2206 16-17 0.7110912368521483 2206 18-19 0.7137406885255544 2206 20-21 0.7031588665028181 2206 22-23 0.7321337226377267 2206 24-25 0.7517654018851943 2206 26-27 0.7691278105737425 2206 28-29 0.7605518241239722 2206 30-31 0.8336738334523659 2206 32-33 0.8321316967309116 2206 34-35 0.8830289537850504 2206 36-37 0.8753035241019091 2206 38-39 0.9136027857296725 2206 40-41 0.9155729039229143 2206 42-43 0.9435261389613885 2206 44-45 0.9810038103825605 2206 46-47 1.0815765152384174 2206 48-49 2.273126617546467 2206 50-51 1.3240139221622869 2206 52-53 1.1054104831352305 2206 54-55 1.1132284526424812 2206 56-57 1.0466206951997137 2206 58-59 1.1021745934808038 2206 60-61 1.0998504268590565 2206 62-63 1.0724676927961596 2206 64-65 1.133862337687006 2206 66-67 1.1650115553125602 2206 68-69 1.1422596121966322 2206 70-71 1.1004630617075932 2206 72-73 1.1557842799682163 2206 74-75 1.1090534756795236 2206 76-77 1.1086787150035846 2206 78-79 0.9168609493216664 2206 80-81 0.8764947069275095 2206 82-83 0.8841446902020706 2206 84-85 0.8168096428895382 2206 86-87 0.9722907791214475 2206 88-89 0.9405560177608514 2206 90-91 0.9500931417425846 2206 92-93 0.9404809555883311 2206 94-95 0.9229073926808766 2206 96-97 0.9554990741002811 2206 98-99 1.0193813125799132 2206 100 1.2506708257188173 2207 1 0.6118707461440991 2207 2 0.5829760911273141 2207 3 0.5575907957040869 2207 4 0.35690012538785965 2207 5 0.40948193283077927 2207 6 0.4515550376786237 2207 7 0.4792108513060711 2207 8 0.48764286256894707 2207 9 0.6123908469361794 2207 10-11 0.659748471296389 2207 12-13 0.6527962464467478 2207 14-15 0.7546170735302411 2207 16-17 0.761715489063306 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0.8594509815859936 2207 86-87 0.9480666664185655 2207 88-89 0.9513706069457548 2207 90-91 1.0357141486002845 2207 92-93 0.9286080641114154 2207 94-95 0.9369807650021862 2207 96-97 0.9524260833494473 2207 98-99 1.099202867339283 2207 100 1.3392639094168644 2208 1 0.5138693330897439 2208 2 0.5186932162145439 2208 3 0.48415317102815436 2208 4 0.3182762285205172 2208 5 0.36611081165774095 2208 6 0.4354482490695233 2208 7 0.4573324465196791 2208 8 0.5037786666679693 2208 9 0.6056529424058468 2208 10-11 0.5887369768100612 2208 12-13 0.5806164795281745 2208 14-15 0.782053105255784 2208 16-17 0.7516428342765451 2208 18-19 0.7324291350980374 2208 20-21 0.7082639827381243 2208 22-23 0.6928445794183844 2208 24-25 0.7659252332482467 2208 26-27 0.7799899103298884 2208 28-29 0.7171869290656261 2208 30-31 0.8130696412906318 2208 32-33 0.8632520251509916 2208 34-35 0.8367158786063982 2208 36-37 0.8874998563339886 2208 38-39 0.8746005706459457 2208 40-41 0.8901792645615103 2208 42-43 0.9354946849500152 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2310 94-95 0.9309899208038175 2310 96-97 0.9754877859882285 2310 98-99 1.0874450335021422 2310 100 1.2795994839173197 2311 1 0.3160537847581111 2311 2 0.3624145029925927 2311 3 0.3597317221257157 2311 4 0.2416287030163744 2311 5 0.3027918381380559 2311 6 0.3549592861807582 2311 7 0.3379556557310863 2311 8 0.18881967668751543 2311 9 0.39491237926306866 2311 10-11 0.3320772831080774 2311 12-13 0.36983208899642506 2311 14-15 0.34771806356580726 2311 16-17 0.3815401939313432 2311 18-19 0.5305128065354054 2311 20-21 0.5867916810566953 2311 22-23 0.6008442382594836 2311 24-25 0.5095967208784273 2311 26-27 0.5525489686603748 2311 28-29 0.641521861371892 2311 30-31 0.5598337313896948 2311 32-33 0.5745700307727262 2311 34-35 0.6542583933909398 2311 36-37 0.6705873177688488 2311 38-39 0.7280180574932871 2311 40-41 0.5767496509047447 2311 42-43 0.5935893154518652 2311 44-45 0.6535029957051179 2311 46-47 0.7792395420718279 2311 48-49 -5.922576727577031 2311 50-51 -1.1386036527991763 2311 52-53 0.2205113724332648 2311 54-55 0.6833804052096895 2311 56-57 0.8200936725643047 2311 58-59 0.7418094716861461 2311 60-61 0.6335705723098002 2311 62-63 0.5474450830835451 2311 64-65 0.5818026481942198 2311 66-67 0.7483315768016325 2311 68-69 0.8033896470443267 2311 70-71 0.8059916989935871 2311 72-73 0.31127131820502996 2311 74-75 0.6014587993181166 2311 76-77 0.6471202949817396 2311 78-79 0.6368091359848407 2311 80-81 0.6739792993496607 2311 82-83 0.6100987836851601 2311 84-85 0.6920534148772788 2311 86-87 0.6900030029665878 2311 88-89 0.7691160987741021 2311 90-91 0.8011254910465659 2311 92-93 0.7701404055644581 2311 94-95 0.8084561845075484 2311 96-97 0.9300963249018146 2311 98-99 0.9533831752726734 2311 100 0.9641158701183663 2312 1 0.006800808574567441 2312 2 0.1604323426108607 2312 3 0.18895374180454638 2312 4 -0.03576593880427481 2312 5 0.18075869742815343 2312 6 0.055874694268140956 2312 7 0.2312084547539044 2312 8 0.33135334305191577 2312 9 0.22188346709981488 2312 10-11 0.15984005791116118 2312 12-13 0.2234099196634176 2312 14-15 0.3020902910588248 2312 16-17 0.46264454625739404 2312 18-19 0.31053586656725685 2312 20-21 0.26711419515378765 2312 22-23 0.5059175618178955 2312 24-25 0.6192268629100326 2312 26-27 0.5162831786284627 2312 28-29 0.6833067323126372 2312 30-31 0.6536413523310287 2312 32-33 0.6941673572775997 2312 34-35 0.2500315367582502 2312 36-37 0.6240160478761467 2312 38-39 0.790658503984929 2312 40-41 0.5399411604772553 2312 42-43 0.6001422224028659 2312 44-45 0.7492599673291025 2312 46-47 0.7643832462227564 2312 48-49 -4.788570377763868 2312 50-51 -0.7322249344637441 2312 52-53 0.5218244597152264 2312 54-55 0.8100182677189807 2312 56-57 0.9373560033000601 2312 58-59 0.9272658586797533 2312 60-61 0.8562072541361445 2312 62-63 0.6440578458816546 2312 64-65 0.8477925686816903 2312 66-67 0.8938070435385441 2312 68-69 0.7829669205874765 2312 70-71 0.8008818651750076 2312 72-73 0.9042396110983191 2312 74-75 0.6485547038751136 2312 76-77 0.8275310980559425 2312 78-79 0.46096342665429546 2312 80-81 0.5960121195385213 2312 82-83 0.5598072843693274 2312 84-85 0.6074802964523371 2312 86-87 0.6758856538037854 2312 88-89 0.7583237183167917 2312 90-91 0.5732230856235851 2312 92-93 0.6559604673842294 2312 94-95 0.779703990395344 2312 96-97 0.8122880885600914 2312 98-99 0.8782922347124291 2312 100 0.5188203613757167 2313 1 0.04933855007216437 2313 2 0.15118493298249547 2313 3 0.2643616082158715 2313 4 0.044636991389374714 2313 5 0.09423748540389454 2313 6 0.24178784883117288 2313 7 0.1021923503437634 2313 8 0.2154330698231277 2313 9 0.3997792672012821 2313 10-11 0.1696705011195192 2313 12-13 0.037951965497107665 2313 14-15 0.10202735999676094 2313 16-17 0.040692346441126404 2313 18-19 0.33142238040922933 2313 20-21 0.29536247204817556 2313 22-23 0.3740749871690312 2313 24-25 0.18701701424166828 2313 26-27 0.36976863963920437 2313 28-29 0.25428714284140597 2313 30-31 0.42786638329665294 2313 32-33 0.39210682919372175 2313 34-35 0.2808134816280159 2313 36-37 -0.3369182741480685 2313 38-39 0.03176388537630004 2313 40-41 0.2677607462920264 2313 42-43 0.41070715651807177 2313 44-45 0.3925075472112738 2313 46-47 0.26870043705783786 2313 48-49 -5.4095774118740465 2313 50-51 -1.3916861897256183 2313 52-53 0.047038733839848135 2313 54-55 0.16222596821854296 2313 56-57 0.18623607035035405 2313 58-59 0.2048478318870579 2313 60-61 0.6129801275632971 2313 62-63 0.6400471690256637 2313 64-65 0.6447937593156752 2313 66-67 0.7440762502243743 2313 68-69 0.7131810004627894 2313 70-71 0.8061054575531159 2313 72-73 0.5957944225393774 2313 74-75 0.6055587625429766 2313 76-77 0.6629523733826233 2313 78-79 0.5721795815692587 2313 80-81 -0.3610672107355768 2313 82-83 0.028508293551603003 2313 84-85 0.3988699674168714 2313 86-87 0.5388671921211774 2313 88-89 0.581751241615283 2313 90-91 0.6118014263688636 2313 92-93 0.6832873455464927 2313 94-95 0.56387629981392 2313 96-97 0.5863288683453618 2313 98-99 0.7815152306309052 2313 100 0.72002607824707 2314 1 0.05215767326886578 2314 2 0.17742210803162806 2314 3 0.1921004073503667 2314 4 -0.012340526886511327 2314 5 0.1809399062656425 2314 6 0.24058253019103404 2314 7 0.09461986926748267 2314 8 0.19382461550961239 2314 9 0.4018949299075416 2314 10-11 0.2876755054768907 2314 12-13 0.12743053790748604 2314 14-15 0.23575623787376543 2314 16-17 0.45084113009527016 2314 18-19 0.4881329040936677 2314 20-21 0.5742234526228032 2314 22-23 0.29813109317792197 2314 24-25 0.38602595178114285 2314 26-27 0.46580828382761297 2314 28-29 0.5450892948249262 2314 30-31 0.45745177326021746 2314 32-33 0.47124078271772873 2314 34-35 0.35218025254860663 2314 36-37 0.47289697487553894 2314 38-39 0.45640196586852255 2314 40-41 0.24964047722225757 2314 42-43 0.3183361397662736 2314 44-45 0.4824535793279381 2314 46-47 0.6179700493109834 2314 48-49 -6.221583475576722 2314 50-51 -1.4204477246468343 2314 52-53 -0.6338111527614814 2314 54-55 -0.2171727019242482 2314 56-57 0.002295464244845391 2314 58-59 0.43703324069407756 2314 60-61 0.6063281129200462 2314 62-63 0.5189170467207092 2314 64-65 0.58712626530248 2314 66-67 0.6735578568802509 2314 68-69 0.6880114974678335 2314 70-71 0.5381630252670035 2314 72-73 0.6612219694888495 2314 74-75 0.7522399570653633 2314 76-77 0.6335009507446827 2314 78-79 0.44879805121666294 2314 80-81 0.5137141989513623 2314 82-83 0.5133090755123746 2314 84-85 0.49022962768276557 2314 86-87 0.6339610551572115 2314 88-89 0.6065292994784635 2314 90-91 0.7328659381320719 2314 92-93 0.7303995874208518 2314 94-95 0.7645595120497113 2314 96-97 0.7544612596096663 2314 98-99 0.7209449938682191 2314 100 -0.579074689405072 2315 1 0.04300108761054844 2315 2 0.13961949473525692 2315 3 0.234709249257385 2315 4 0.0742012241149439 2315 5 0.18793473676928585 2315 6 0.26295593945205553 2315 7 0.31871412656187914 2315 8 0.23713994502508484 2315 9 0.3646510774270908 2315 10-11 0.20012898914452393 2315 12-13 0.33992264601239697 2315 14-15 0.2806804889299599 2315 16-17 0.3035306014739305 2315 18-19 0.34108230071148427 2315 20-21 0.3383598118787461 2315 22-23 0.31971953170328504 2315 24-25 0.34134257175308136 2315 26-27 0.3828580685586189 2315 28-29 0.40733289278435336 2315 30-31 0.43467505277130414 2315 32-33 0.5556400245379436 2315 34-35 0.5120283299944006 2315 36-37 0.3926096771203831 2315 38-39 0.46917491177575243 2315 40-41 0.49487645377620026 2315 42-43 0.5267763254424978 2315 44-45 0.4959491731854584 2315 46-47 0.3280946934871025 2315 48-49 -4.939168619135646 2315 50-51 -1.0101888572388944 2315 52-53 0.13397123761910734 2315 54-55 0.10039595355343778 2315 56-57 0.37593421258157633 2315 58-59 0.5020238945425533 2315 60-61 0.3668689094327888 2315 62-63 0.18018273654730166 2315 64-65 0.3447329748114285 2315 66-67 -0.3472283174260653 2315 68-69 0.37283289901819927 2315 70-71 0.3444927644380371 2315 72-73 0.37900720841296476 2315 74-75 0.5599143168682801 2315 76-77 0.552555977632565 2315 78-79 0.3838328494707426 2315 80-81 0.27501529857980955 2315 82-83 0.30266519101108713 2315 84-85 0.3289177928455729 2315 86-87 0.33045309994565386 2315 88-89 0.3206911875419287 2315 90-91 0.3193584061924746 2315 92-93 0.3396129731069557 2315 94-95 -0.010717825216865151 2315 96-97 0.16305468786283583 2315 98-99 0.22431269056171743 2315 100 0.3111522750126099 2316 1 0.2546962385267122 2316 2 0.2584931511891 2316 3 0.18246752246773923 2316 4 0.01401615780142862 2316 5 0.18637545779814957 2316 6 0.16990797324260143 2316 7 0.25729868057110394 2316 8 0.3121408159368144 2316 9 0.5172247152691014 2316 10-11 0.4424125377399406 2316 12-13 0.3206747681571116 2316 14-15 0.4862649623017745 2316 16-17 0.43301140762036283 2316 18-19 0.1466977868682804 2316 20-21 0.41616527878944254 2316 22-23 0.48896019938099755 2316 24-25 0.5754381448428347 2316 26-27 0.6238160964425035 2316 28-29 0.6360493021353477 2316 30-31 0.5515206472695269 2316 32-33 0.4718402040498546 2316 34-35 0.5312789663151278 2316 36-37 0.3357569758436654 2316 38-39 0.46412074384204516 2316 40-41 0.08857692717744214 2316 42-43 0.042871416095735526 2316 44-45 0.29898063648510487 2316 46-47 0.37808124466694437 2316 48-49 -5.020342935692007 2316 50-51 -0.966630380616877 2316 52-53 0.17703679902129466 2316 54-55 0.52698779282602 2316 56-57 0.4445454713120256 2316 58-59 0.23395511319580464 2316 60-61 0.5281728995408983 2316 62-63 0.36828091584908407 2316 64-65 0.43076827997682443 2316 66-67 0.4367207932932544 2316 68-69 0.14311700569107444 2316 70-71 -0.15661074317803525 2316 72-73 -0.05684077439585877 2316 74-75 0.12329823155196351 2316 76-77 0.07566383342290806 2316 78-79 0.18526469774806742 2316 80-81 0.06022381358440754 2316 82-83 0.041930475303736614 2316 84-85 0.21598070098569622 2316 86-87 0.2820364452265558 2316 88-89 0.05508769359674304 2316 90-91 0.0931108234062421 2316 92-93 0.1257493387305466 2316 94-95 0.3360602628661056 2316 96-97 0.43330583174120463 2316 98-99 0.4257353508937918 2316 100 -0.38155231603153084 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 1.0 10 8.0 11 7.0 12 26.0 13 136.0 14 559.0 15 1660.0 16 3933.0 17 8543.0 18 14322.0 19 18982.0 20 23115.0 21 30784.0 22 37246.0 23 35978.0 24 33861.0 25 36254.0 26 43205.0 27 53346.0 28 66113.0 29 81789.0 30 100784.0 31 125750.0 32 158896.0 33 205627.0 34 282756.0 35 399819.0 36 618039.0 37 1042979.0 38 1053068.0 39 149473.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.22771385093717 22.237367119738106 14.306480313086468 22.22843871623826 2 14.741318367203151 22.63292198519942 41.827165488684734 20.79859415891269 3 16.233703783667895 31.164877305751304 31.318775959997158 21.282642950583643 4 10.952762850138996 19.345311952823376 42.07368320932465 27.628241987712983 5 10.829931055379381 39.29188529963063 36.3468103954855 13.531373249504488 6 25.1310709815903 41.17408564766538 21.114968361208046 12.579875009536265 7 25.243117952486184 33.425328096043394 25.68015430914779 15.651399642322634 8 24.569312252561218 35.68391982367468 22.85455797421255 16.892209949551557 9 26.30890366383442 16.988086934663265 23.175242472821843 33.52776692868047 10-11 21.985379374436057 28.149599364602523 31.189889832019496 18.675131428941924 12-13 23.204747710350336 26.64120780471886 30.437495846455832 19.716548638474976 14-15 21.403004493124158 27.86661969669791 28.122537588581974 22.607838221595955 16-17 19.43458636216773 30.48019744740445 29.471867094728722 20.6133490956991 18-19 19.178994926057456 29.544620275563005 31.60280069093363 19.673584107445908 20-21 19.892705330510957 29.30793900806136 31.530511583949284 19.268844077478395 22-23 20.18549555711882 28.82952800533383 30.993329464790314 19.991646972757028 24-25 19.83353843055028 29.30488036283837 31.35150853433509 19.51007267227626 26-27 19.37829359239424 29.365668976587028 31.542499397296975 19.71353803372176 28-29 19.398464586107796 28.278758321219723 32.33472213037057 19.98805496230192 30-31 19.997093120637654 28.89838997785801 31.69696917303064 19.40754772847369 32-33 19.824115375990534 29.29131194799651 31.54507752702436 19.339495148988593 34-35 19.956227691659215 29.172643928483616 31.53826159088023 19.332866788976943 36-37 20.296836598498448 28.7421068440119 30.82780336399248 20.133253193497172 38-39 19.516320983760675 29.63834750532551 31.313911364999925 19.531420145913895 40-41 19.565523261707256 28.811029872788264 32.26466239580121 19.358784469703274 42-43 19.608704903486515 28.739784740796665 31.21799854565983 20.43351181005699 44-45 19.253037747861065 29.297023046541465 31.22188914053433 20.22805006506314 46-47 19.63166759518883 28.800617741924157 30.997070697191358 20.570643965695652 48-49 19.53450012742783 29.31289602436114 30.751583550544005 20.40102029766703 50-51 19.66631987803531 28.798140699922204 30.680504779565855 20.855034642476628 52-53 19.593131933009705 29.299321202046823 30.499065916517743 20.60848094842573 54-55 19.966416225687805 29.289134077674962 30.345548896293835 20.3989008003434 56-57 19.86605210662532 29.49924941500191 29.991790088214675 20.64290839015809 58-59 19.320062096463495 29.01459958533914 30.796420105378107 20.868918212819256 60-61 20.086355067268013 30.093006493110018 29.871731764367702 19.948906675254264 62-63 19.63190561955216 29.484088191183343 30.826064506591166 20.057941682673324 64-65 19.599344861590446 28.891997272922605 30.422444089077032 21.086213776409917 66-67 19.65670984852481 29.218489364222734 29.762222345206396 21.36257844204606 68-69 19.32689905766603 29.463969256082677 30.20995437334775 20.99917731290354 70-71 19.88332247221118 28.790714480968973 30.51196364584794 20.813999400971912 72-73 19.720178664555405 29.2524785248555 29.716833432861634 21.310509377727456 74-75 19.612380364276607 29.53262717789917 29.645527464910348 21.20946499291388 76-77 19.49745662229801 29.10778978116263 30.483019669717002 20.911733926822354 78-79 19.412290110365138 29.030629364961435 29.805860024045284 21.75122050062815 80-81 19.306565099443436 29.84156740765843 29.20194547079805 21.64992202210008 82-83 20.2941802670102 29.239443499988653 29.319462261816113 21.146913971185032 84-85 19.41342471341314 29.52090850026919 29.117121475604975 21.9485453107127 86-87 19.15607808346482 29.662710958321632 29.978255385407564 21.202955572805983 88-89 19.801081609291074 29.040009598931142 29.746514712415806 21.412394079361974 90-91 19.9116386186598 29.196554950200127 29.715964753153617 21.175841677986458 92-93 19.992752572210037 29.362662972560717 29.39920271809883 21.245381737130412 94-95 19.503567444383194 28.459239882496902 29.798878116198303 22.238314556921605 96-97 20.139616444348533 28.728030196521832 29.49543543336502 21.636917925764614 98-99 19.517843583082183 28.81827128609845 29.653067078280653 22.010818052538713 100 19.619542034772113 28.29304793364773 29.677152523699167 22.41025750788099 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3220.0 1 2218.0 2 3365.5 3 4628.5 4 2793.0 5 1934.5 6 2474.5 7 3925.0 8 5785.5 9 6703.0 10 5911.5 11 4849.0 12 4784.5 13 5106.0 14 5551.5 15 5968.0 16 6298.5 17 6834.0 18 7389.0 19 7823.5 20 8546.5 21 9565.0 22 10696.0 23 12358.5 24 13807.5 25 16358.5 26 23017.0 27 30313.0 28 33636.5 29 42093.0 30 61029.5 31 78066.5 32 102664.0 33 138433.0 34 174325.0 35 204685.0 36 240524.5 37 271582.5 38 270653.0 39 261449.0 40 241118.5 41 213752.5 42 192005.5 43 179064.5 44 179085.0 45 180192.0 46 166318.5 47 141964.5 48 128085.0 49 119906.5 50 112672.0 51 99771.5 52 86154.0 53 77843.5 54 70739.0 55 63259.5 56 55499.0 57 46847.5 58 38745.0 59 32298.0 60 25656.5 61 19749.5 62 15201.5 63 11393.5 64 8019.0 65 5565.0 66 4000.5 67 2981.5 68 2298.5 69 1713.5 70 1310.0 71 1038.5 72 863.0 73 716.5 74 529.0 75 345.0 76 216.0 77 144.5 78 95.5 79 56.0 80 36.5 81 22.0 82 11.5 83 8.0 84 10.0 85 6.5 86 1.5 87 1.0 88 1.5 89 1.5 90 1.0 91 0.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029997352539131095 2 0.002031520993284094 3 8.644770184187634E-5 4 0.022800581360794887 5 6.051339128931345E-4 6 4.322385092093817E-5 7 0.0 8 0.003738863104661152 9 0.010244052668262347 10-11 0.0010805962730234544 12-13 5.402981365117272E-5 14-15 1.0805962730234543E-4 16-17 4.3223850920938174E-4 18-19 5.402981365117272E-5 20-21 9.725366457211088E-5 22-23 2.1611925460469087E-4 24-25 2.1611925460469086E-5 26-27 7.56417391116418E-5 28-29 0.0010589843475629853 30-31 0.00259343105525629 32-33 0.009995515525466953 34-35 0.018640285709654588 36-37 0.021493059870436506 38-39 0.02140661216859463 40-41 0.0047978474522241375 42-43 0.005067996520480001 44-45 0.008979755028824905 46-47 0.011832529189606825 48-49 1.2072421562218032 50-51 0.01767855502666371 52-53 0.01907252421886397 54-55 0.009422799500764523 56-57 0.007574979873894415 58-59 0.008698799997838806 60-61 0.005348951551466099 62-63 0.007650621613006057 64-65 0.01895365862883139 66-67 0.029543502104461244 68-69 0.029813651172717105 70-71 0.026982488937395653 72-73 0.023502968938260132 74-75 0.00920668024615983 76-77 0.014015333661114203 78-79 0.008374621115931772 80-81 0.002658266831637698 82-83 0.014263870803909597 84-85 0.02423777440391608 86-87 0.023816341857436935 88-89 0.03371460371833178 90-91 0.020725836516589855 92-93 0.013096826829044268 94-95 0.006559219377252368 96-97 0.003987400247456547 98-99 0.015863153287984308 100 0.03678349713371838 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4627075.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.532311873185954 #Duplication Level Percentage of deduplicated Percentage of total 1 81.6304609689351 40.43345451065227 2 12.426687502952207 12.310451218937022 3 3.0051381667839583 4.46554322697472 4 1.0719032884660993 2.123753919287857 5 0.5371372220516207 1.3302824200678809 6 0.2926630388391708 0.8697766148121684 7 0.1847151822247332 0.6404559009567502 8 0.13389364384337415 0.5305649379749835 9 0.08789879869863758 0.39184476393773776 >10 0.4489636154665853 4.158966182700171 >50 0.05968817957246703 2.1166107470196853 >100 0.08756994005387037 10.073512981808562 >500 0.0198741922509658 6.887902978018107 >1k 0.012935864778574842 11.395910634019726 >5k 2.9399692678579186E-4 0.9736094269695498 >10k+ 1.7639815607147513E-4 1.2973595358628316 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18778 0.40582873629668853 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15726 0.33986913979133687 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10041 0.21700534354857007 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8418 0.18192918852622877 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 8178 0.1767423264157162 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 6349 0.13721411474851825 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 5430 0.11735275525034715 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 5054 0.10922667127721075 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 4924 0.10641712096734977 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 4907 0.1060497182345218 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 4877 0.10540136047070772 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 4660 0.10071157264578594 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.0805962730234543E-5 0.0 16-17 0.0 0.0 0.0 4.322385092093817E-5 0.0 18-19 0.0 0.0 0.0 1.0805962730234543E-4 0.0 20-21 0.0 0.0 0.0 4.2143254647914716E-4 0.0 22-23 0.0 0.0 0.0 0.0015236407449630705 1.0805962730234543E-5 24-25 0.0 0.0 0.0 0.0037280571419309174 2.1611925460469086E-5 26-27 0.0 0.0 0.0 0.006915816147350108 2.1611925460469086E-5 28-29 0.0 0.0 0.0 0.01315085664269544 2.1611925460469086E-5 30-31 0.0 0.0 0.0 0.030332337383768365 2.1611925460469086E-5 32-33 0.0 0.0 0.0 0.06858544544879865 2.1611925460469086E-5 34-35 0.0 0.0 0.0 0.12464678009325544 2.1611925460469086E-5 36-37 0.0 0.0 0.0 0.2110620640469411 2.1611925460469086E-5 38-39 0.0 0.0 0.0 0.3311703397934981 2.1611925460469086E-5 40-41 0.0 0.0 0.0 0.46579102348676 2.1611925460469086E-5 42-43 0.0 0.0 0.0 0.6020758254404781 4.322385092093817E-5 44-45 0.0 0.0 0.0 0.7645975049032057 4.322385092093817E-5 46-47 0.0 1.0805962730234543E-5 0.0 0.9622493692019256 4.322385092093817E-5 48-49 0.0 2.1611925460469086E-5 0.0 1.1671412285299028 4.322385092093817E-5 50-51 0.0 2.1611925460469086E-5 0.0 1.3789380980425 4.322385092093817E-5 52-53 0.0 2.1611925460469086E-5 0.0 1.593036637616637 5.402981365117272E-5 54-55 0.0 2.1611925460469086E-5 0.0 1.8358250082395466 6.483577638140726E-5 56-57 0.0 2.1611925460469086E-5 0.0 2.114391921462263 7.56417391116418E-5 58-59 0.0 2.1611925460469086E-5 0.0 2.4165590572878113 8.644770184187634E-5 60-61 0.0 3.241788819070363E-5 0.0 2.724399323546733 8.644770184187634E-5 62-63 0.0 4.322385092093817E-5 0.0 3.0305322476942775 8.644770184187634E-5 64-65 0.0 4.322385092093817E-5 0.0 3.351231609602179 8.644770184187634E-5 66-67 0.0 4.322385092093817E-5 0.0 3.705915724296667 8.644770184187634E-5 68-69 0.0 4.322385092093817E-5 0.0 4.088284715506016 8.644770184187634E-5 70-71 0.0 5.402981365117272E-5 0.0 4.505286817265768 8.644770184187634E-5 72-73 0.0 6.483577638140726E-5 0.0 4.906134004743818 8.644770184187634E-5 74-75 0.0 6.483577638140726E-5 0.0 5.314534560170302 8.644770184187634E-5 76-77 0.0 6.483577638140726E-5 0.0 5.747767217950866 8.644770184187634E-5 78-79 0.0 6.483577638140726E-5 0.0 6.216616328890281 8.644770184187634E-5 80-81 0.0 6.483577638140726E-5 0.0 6.698670326286045 8.644770184187634E-5 82-83 0.0 6.483577638140726E-5 0.0 7.184430768898277 1.0805962730234543E-4 84-85 0.0 6.483577638140726E-5 0.0 7.664172290269771 1.0805962730234543E-4 86-87 0.0 6.483577638140726E-5 0.0 8.169426689647347 1.0805962730234543E-4 88 0.0 6.483577638140726E-5 0.0 8.55836570619668 1.0805962730234543E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 5465 0.0 68.42263 2 ACCTGGG 5695 0.0 64.02062 3 GTACCTG 6840 0.0 55.09273 1 CCTGGGG 3020 0.0 48.943634 4 GGTATCA 9880 0.0 46.975803 1 TATCACG 245 0.0 44.044666 2 TAACGCA 405 0.0 44.02721 4 GTATCAA 17685 0.0 37.707165 1 GTACCCG 340 0.0 33.125862 1 ATCAACG 20970 0.0 30.566439 3 CAACGCA 21290 0.0 30.10115 5 TCAACGC 21575 0.0 29.709307 4 GGATGAC 4080 0.0 29.666592 8 AACGCAG 22365 0.0 28.927004 6 TACGTGG 430 0.0 28.368505 2 TATCAAC 23425 0.0 27.55944 2 TATAACG 630 0.0 27.554512 2 GGGTATG 2205 0.0 26.808348 7 GTACATG 21055 0.0 26.768412 1 GTACACG 405 0.0 26.65064 1 >>END_MODULE