FastQCFastQC Report
Mon 6 Jun 2016
ERR1211302_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211302_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7389358
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284930.3855950679341832No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221670.2999854655844256No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126180.17075908353607988No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123950.053.2024041
GTATCAA235100.042.9945641
TACCTGG59700.035.6888922
ATCAACG288300.034.6030543
CAACGCA290400.034.1905485
TATCAAC297950.033.6868362
TCAACGC299600.033.219514
AACGCAG306850.032.6183936
GTACCTG74850.028.780841
ACGCAGA345200.028.6955647
ACCTGGG72100.028.4158273
CGCAGAG351550.028.0420558
GTACATG301800.026.3700451
TACATGG310900.025.1733932
GCAGAGT384650.025.1177739
GTGGTAT59900.024.8136161
ACATGGG312900.024.3286783
TGGTATC60600.023.2840122
CATGGGG197100.022.8634134
GAGTACT223450.021.04064412-13
Produced by FastQC (version 0.11.5)