##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211302_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7389358 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.386365364893674 31.0 31.0 34.0 28.0 34.0 2 31.401310370941562 31.0 31.0 34.0 28.0 34.0 3 31.447275257200964 33.0 31.0 34.0 28.0 34.0 4 35.053522241039076 37.0 35.0 37.0 32.0 37.0 5 35.10310882217372 37.0 35.0 37.0 32.0 37.0 6 35.16568205248684 37.0 35.0 37.0 32.0 37.0 7 35.104204722521224 37.0 35.0 37.0 32.0 37.0 8 35.10419389614091 37.0 35.0 37.0 32.0 37.0 9 36.66306057982304 39.0 37.0 39.0 32.0 39.0 10-11 36.59559619387774 39.0 37.0 39.0 32.0 39.0 12-13 36.5135457640569 39.0 37.0 39.0 32.0 39.0 14-15 37.64571252333423 40.0 37.0 41.0 32.0 41.0 16-17 37.644880380677186 40.0 37.0 41.0 32.0 41.0 18-19 37.654558149706645 40.0 37.0 41.0 32.0 41.0 20-21 37.62327187287448 40.0 37.0 41.0 32.0 41.0 22-23 37.502602526498244 40.0 37.0 41.0 31.5 41.0 24-25 37.39366904404957 40.0 37.0 41.0 31.0 41.0 26-27 37.25066555172994 40.0 36.5 41.0 31.0 41.0 28-29 37.11392884199142 40.0 36.0 41.0 30.5 41.0 30-31 36.928695835280955 39.0 36.0 41.0 30.0 41.0 32-33 36.79692958441045 39.0 36.0 41.0 30.0 41.0 34-35 36.647673113144606 39.0 35.0 41.0 30.0 41.0 36-37 36.548037596770925 39.0 35.0 41.0 30.0 41.0 38-39 36.40123377430082 39.0 35.0 41.0 29.0 41.0 40-41 36.23685677429623 39.0 35.0 40.5 28.5 41.0 42-43 36.08913886700306 39.0 35.0 40.0 28.0 41.0 44-45 35.95242719326902 39.0 35.0 40.0 27.0 41.0 46-47 35.780710854718365 39.0 35.0 40.0 26.5 41.0 48-49 35.5402129657272 38.5 34.0 40.0 26.0 41.0 50-51 34.87273082451818 37.5 33.5 39.5 25.0 40.5 52-53 35.116439411921846 38.0 34.0 40.0 26.0 40.5 54-55 35.48060149474419 38.0 34.0 40.0 26.0 41.0 56-57 35.39028180797303 38.0 34.0 40.0 26.0 41.0 58-59 35.17605169488337 38.0 34.0 40.0 26.0 41.0 60-61 34.92197332975341 38.0 34.0 40.0 25.5 41.0 62-63 34.65796277565656 37.0 33.5 40.0 25.0 41.0 64-65 34.38765505744884 37.0 33.0 40.0 25.0 41.0 66-67 34.16493388194211 36.0 33.0 39.5 24.5 41.0 68-69 33.83054028509649 36.0 33.0 39.0 23.5 41.0 70-71 33.48666487670512 35.0 32.0 39.0 23.5 40.5 72-73 33.12114611309941 35.0 32.0 38.0 23.0 40.0 74-75 32.74549872938894 35.0 32.0 37.0 22.0 39.5 76-77 32.386827313008794 35.0 31.0 37.0 21.5 39.0 78-79 32.01561400868655 35.0 31.0 36.0 20.5 39.0 80-81 31.672625889826964 35.0 31.0 36.0 20.0 38.0 82-83 31.352104878394037 34.5 30.5 35.5 20.0 37.0 84-85 31.036797175072586 34.0 30.0 35.0 19.0 37.0 86-87 30.774843024251904 34.0 30.0 35.0 18.5 36.0 88-89 30.505194564940552 34.0 29.5 35.0 18.0 36.0 90-91 30.2407079207693 34.0 29.0 35.0 17.5 36.0 92-93 30.00044340793882 34.0 29.0 35.0 17.0 35.0 94-95 29.774822589459056 34.0 29.0 35.0 15.0 35.0 96-97 29.56651998725735 34.0 29.0 35.0 15.0 35.0 98-99 29.327820427701567 34.0 27.5 35.0 12.0 35.0 100 27.658548009177522 32.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.33829545755827084 1101 2 -0.394812625053266 1101 3 -0.3838056231874454 1101 4 -0.2769833027305424 1101 5 -0.2609347381836926 1101 6 -0.3529400009048089 1101 7 -0.43805861585909867 1101 8 -0.4751899408881499 1101 9 -0.4764905909063444 1101 10-11 -0.5365150219969124 1101 12-13 -0.43770638942566364 1101 14-15 -0.48438338327730435 1101 16-17 -0.502421530145071 1101 18-19 -0.6382893976960133 1101 20-21 -0.6523323037000281 1101 22-23 -0.6874001288490845 1101 24-25 -0.6319412565846605 1101 26-27 -0.7006320517075721 1101 28-29 -0.6879528766674738 1101 30-31 -0.6749227995513962 1101 32-33 -0.7626412300304608 1101 34-35 -0.7468997776093005 1101 36-37 -0.8210674758085474 1101 38-39 -0.824061470376293 1101 40-41 -0.8047555916469236 1101 42-43 -0.7720048859065471 1101 44-45 -0.8574574398269661 1101 46-47 -0.7792237497153138 1101 48-49 -0.831767399123315 1101 50-51 -0.7430334139415109 1101 52-53 -0.5546133450636646 1101 54-55 -0.6131868991701026 1101 56-57 -0.526603462691007 1101 58-59 -0.5541518117901347 1101 60-61 -0.6338071002816292 1101 62-63 -0.5610673562677491 1101 64-65 -0.5892238057271015 1101 66-67 -0.7472190519969288 1101 68-69 -0.6839206470406296 1101 70-71 -0.6153843742082543 1101 72-73 -0.6565254441665687 1101 74-75 -0.7127683765605219 1101 76-77 -0.7074613778579746 1101 78-79 -0.7568792911011109 1101 80-81 -0.8222168682114521 1101 82-83 -0.9156959254633961 1101 84-85 -0.9240184195210972 1101 86-87 -0.9551786525221644 1101 88-89 -1.0393834358355925 1101 90-91 -0.9749267300026467 1101 92-93 -0.9688587885039439 1101 94-95 -1.0311438974644211 1101 96-97 -1.119066336730885 1101 98-99 -1.115496598543821 1101 100 -1.078150322959953 1102 1 -0.3479752899065893 1102 2 -0.3363387301008167 1102 3 -0.35756395458846413 1102 4 -0.20080729259385066 1102 5 -0.27705039893717753 1102 6 -0.2059143164277515 1102 7 -0.22986063337809526 1102 8 -0.262852091803488 1102 9 -0.28691699917010993 1102 10-11 -0.36019278866686477 1102 12-13 -0.3311672103157619 1102 14-15 -0.47697885206889623 1102 16-17 -0.5058081228148268 1102 18-19 -0.46385158259264614 1102 20-21 -0.45497138760068623 1102 22-23 -0.5301777507802186 1102 24-25 -0.5873532867299218 1102 26-27 -0.5490335746676323 1102 28-29 -0.61590844948023 1102 30-31 -0.5575467694994671 1102 32-33 -0.6462158812557632 1102 34-35 -0.5901323434310157 1102 36-37 -0.6516522481512084 1102 38-39 -0.8303964083974265 1102 40-41 -0.7786901634465266 1102 42-43 -0.7670853755568956 1102 44-45 -0.9539716522831583 1102 46-47 -0.8502571617537811 1102 48-49 -0.854227606505944 1102 50-51 -0.8244496486499102 1102 52-53 -0.7137397463665778 1102 54-55 -0.698464167726371 1102 56-57 -0.645514090852032 1102 58-59 -0.6937722433359852 1102 60-61 -0.66028341496596 1102 62-63 -0.7500684925610486 1102 64-65 -0.8088358234931974 1102 66-67 -0.705429513819162 1102 68-69 -0.6534618120946831 1102 70-71 -0.7660821411957599 1102 72-73 -0.8653678281587318 1102 74-75 -0.8674129546890939 1102 76-77 -0.8741416100407235 1102 78-79 -0.758599867480573 1102 80-81 -0.8537328870213123 1102 82-83 -0.9116549348831278 1102 84-85 -0.932930500391624 1102 86-87 -0.8669900619168764 1102 88-89 -0.9968164944841007 1102 90-91 -0.9406897516108366 1102 92-93 -0.9700355090528525 1102 94-95 -1.0826932391853639 1102 96-97 -0.9961127467168751 1102 98-99 -1.0307168986111677 1102 100 -1.1636448731664721 1103 1 -0.34649234798665063 1103 2 -0.42937327871871744 1103 3 -0.4256543526149166 1103 4 -0.33724587292529407 1103 5 -0.2726223508719485 1103 6 -0.34312079104792303 1103 7 -0.3400619377638989 1103 8 -0.35866330197279694 1103 9 -0.4729119438073468 1103 10-11 -0.44147825532761686 1103 12-13 -0.6093210359966719 1103 14-15 -0.7184199000817273 1103 16-17 -0.6838702471091551 1103 18-19 -0.6276267307839376 1103 20-21 -0.7325216366611969 1103 22-23 -0.7318946860781921 1103 24-25 -0.7455307060993164 1103 26-27 -0.794829706701691 1103 28-29 -0.7909457024119249 1103 30-31 -0.7807057683641148 1103 32-33 -0.7487090461315944 1103 34-35 -0.8806051189614621 1103 36-37 -0.9003872794345256 1103 38-39 -0.9243657221636639 1103 40-41 -0.9029449259791562 1103 42-43 -0.9923459369812093 1103 44-45 -0.8857634662984921 1103 46-47 -0.8672829307392291 1103 48-49 -0.9092191647971646 1103 50-51 -0.9089042089653887 1103 52-53 -0.7997983931263306 1103 54-55 -0.8092844339110883 1103 56-57 -0.8609739634121283 1103 58-59 -0.8389322609679652 1103 60-61 -0.7505393431184473 1103 62-63 -0.8192442059804748 1103 64-65 -0.8078038279785389 1103 66-67 -0.7954248843991323 1103 68-69 -0.797706191502698 1103 70-71 -0.8513654762059062 1103 72-73 -0.9550628135832042 1103 74-75 -0.8501041336355151 1103 76-77 -0.8785245260479684 1103 78-79 -0.8438138811463496 1103 80-81 -0.7512709767284349 1103 82-83 -0.9700971972245434 1103 84-85 -0.8728200577006149 1103 86-87 -0.9078059610439873 1103 88-89 -1.0491314278996242 1103 90-91 -1.0216905154332245 1103 92-93 -1.0383896451357906 1103 94-95 -1.158434285897382 1103 96-97 -1.2916464205916327 1103 98-99 -1.2675817584386557 1103 100 -1.293949169326801 1104 1 -0.38458242942115106 1104 2 -0.4996655854114387 1104 3 -0.47096750611353144 1104 4 -0.27781963391345244 1104 5 -0.25393027808217283 1104 6 -0.28697180375502995 1104 7 -0.3132695685053619 1104 8 -0.33800631365394196 1104 9 -0.43538539640103124 1104 10-11 -0.38901446741870416 1104 12-13 -0.49063990173431904 1104 14-15 -0.5776784083810682 1104 16-17 -0.5840023199448581 1104 18-19 -0.5653614547317787 1104 20-21 -0.534894568317732 1104 22-23 -0.4926223382117314 1104 24-25 -0.6779786628473232 1104 26-27 -0.7150935838237586 1104 28-29 -0.6976849947463961 1104 30-31 -0.7744188867216337 1104 32-33 -0.723807763415337 1104 34-35 -0.6896612965262889 1104 36-37 -0.7265808587287239 1104 38-39 -0.735253578560787 1104 40-41 -0.7338966653198611 1104 42-43 -0.7272242786041616 1104 44-45 -0.7333268933044579 1104 46-47 -0.8665469045478886 1104 48-49 -0.6709875119187529 1104 50-51 -0.639236778626227 1104 52-53 -0.5954668555253804 1104 54-55 -0.5841349881850206 1104 56-57 -0.5194308673587855 1104 58-59 -0.6047033074522261 1104 60-61 -0.4790987582709647 1104 62-63 -0.5104993778191513 1104 64-65 -0.5853557767082265 1104 66-67 -0.5558096944174764 1104 68-69 -0.561505542470158 1104 70-71 -0.5713562898746645 1104 72-73 -0.5847076628088601 1104 74-75 -0.5798324120455689 1104 76-77 -0.5625241613862215 1104 78-79 -0.5603259206598565 1104 80-81 -0.5736264977298582 1104 82-83 -0.7079157346296938 1104 84-85 -0.6799518119431482 1104 86-87 -0.8021259280548172 1104 88-89 -0.7550171286140035 1104 90-91 -0.7121060619848194 1104 92-93 -0.7868999581596512 1104 94-95 -0.7989832767414846 1104 96-97 -0.8416394771774982 1104 98-99 -0.8399509167117927 1104 100 -0.8770712224890573 1105 1 -0.30924127055063266 1105 2 -0.33790888944798425 1105 3 -0.31208771555439796 1105 4 -0.2176375485876747 1105 5 -0.20428110222130158 1105 6 -0.2134263866097328 1105 7 -0.29148064429410425 1105 8 -0.2699794710284493 1105 9 -0.35732400671813025 1105 10-11 -0.37374614267850603 1105 12-13 -0.3976046975027572 1105 14-15 -0.4757101450839798 1105 16-17 -0.5149892993480591 1105 18-19 -0.5263603273842463 1105 20-21 -0.490329302375919 1105 22-23 -0.5355165545449125 1105 24-25 -0.4230489056811848 1105 26-27 -0.45028319726644384 1105 28-29 -0.5846150190564998 1105 30-31 -0.5759375680990217 1105 32-33 -0.4869168703530633 1105 34-35 -0.5865268002262312 1105 36-37 -0.5828626849248693 1105 38-39 -0.5722268374500032 1105 40-41 -0.6569780320283769 1105 42-43 -0.5588801758071682 1105 44-45 -0.5754140463512343 1105 46-47 -0.5477501102754516 1105 48-49 -0.5872114854180737 1105 50-51 -0.5462122067244266 1105 52-53 -0.46158419584008215 1105 54-55 -0.3878821738233569 1105 56-57 -0.3815497036796742 1105 58-59 -0.37266771319048075 1105 60-61 -0.38556131270155447 1105 62-63 -0.4077433710871219 1105 64-65 -0.4110191539422203 1105 66-67 -0.4652295111567142 1105 68-69 -0.44723762913165643 1105 70-71 -0.34166152856779775 1105 72-73 -0.3569818166303591 1105 74-75 -0.4325106050837988 1105 76-77 -0.44166694983491794 1105 78-79 -0.4483314986258229 1105 80-81 -0.5419958937173526 1105 82-83 -0.43751646126127497 1105 84-85 -0.38915535961498904 1105 86-87 -0.45410723866310576 1105 88-89 -0.474107108043917 1105 90-91 -0.5410506509838306 1105 92-93 -0.6416487626904157 1105 94-95 -0.6442001495542442 1105 96-97 -0.7092313132579626 1105 98-99 -0.7400560325187016 1105 100 -0.6903125870604256 1106 1 -0.4244847781566783 1106 2 -0.4502770323273779 1106 3 -0.41637941380474786 1106 4 -0.28425309295193557 1106 5 -0.20375824255669528 1106 6 -0.3287353113799938 1106 7 -0.35778621513674125 1106 8 -0.3530524994278039 1106 9 -0.4968325472923496 1106 10-11 -0.5171058540122786 1106 12-13 -0.4789122931623808 1106 14-15 -0.585927735032584 1106 16-17 -0.6417695703620296 1106 18-19 -0.5923878648952794 1106 20-21 -0.6012231915820863 1106 22-23 -0.5895809125740925 1106 24-25 -0.680928050066214 1106 26-27 -0.6254292125304488 1106 28-29 -0.687802434040151 1106 30-31 -0.7667872332254433 1106 32-33 -0.6952635605560644 1106 34-35 -0.7565375057970769 1106 36-37 -0.7266577007599722 1106 38-39 -0.7735460835950647 1106 40-41 -0.779967669118875 1106 42-43 -0.7933688627912332 1106 44-45 -0.8769252451467295 1106 46-47 -0.8522380288674256 1106 48-49 -0.8723639177943809 1106 50-51 -0.8451725203114719 1106 52-53 -0.6774898186010958 1106 54-55 -0.7543593660771393 1106 56-57 -0.7998353690137705 1106 58-59 -0.6840390230913158 1106 60-61 -0.671751925857663 1106 62-63 -0.6262714952758941 1106 64-65 -0.649047688286231 1106 66-67 -0.7086927788998238 1106 68-69 -0.5818196134523035 1106 70-71 -0.6359343077589088 1106 72-73 -0.6310811468832043 1106 74-75 -0.6917046967716658 1106 76-77 -0.6159876496947376 1106 78-79 -0.5962465809164605 1106 80-81 -0.6716115834530889 1106 82-83 -0.7662819459193884 1106 84-85 -0.7597981715146851 1106 86-87 -0.6361370501993733 1106 88-89 -0.7650212142689341 1106 90-91 -0.7808345355173429 1106 92-93 -0.8474322630278834 1106 94-95 -0.8127745018724006 1106 96-97 -0.8230432441026743 1106 98-99 -0.7623598277525936 1106 100 -0.8293610269129772 1107 1 -0.49521245566224437 1107 2 -0.4871717468895653 1107 3 -0.4793998385090745 1107 4 -0.3158312858452419 1107 5 -0.3678144181290861 1107 6 -0.40512918341040205 1107 7 -0.3610672614722148 1107 8 -0.4205798369649898 1107 9 -0.5772485192383883 1107 10-11 -0.5934392578439613 1107 12-13 -0.6355485732934412 1107 14-15 -0.7308705821167578 1107 16-17 -0.7093728112932709 1107 18-19 -0.7028177794204211 1107 20-21 -0.7005925571200251 1107 22-23 -0.7893095076527601 1107 24-25 -0.7454174455944127 1107 26-27 -0.7179089801092431 1107 28-29 -0.7736606670255526 1107 30-31 -0.7072703926063184 1107 32-33 -0.7197559314644622 1107 34-35 -0.7326458759750878 1107 36-37 -0.7756619007751482 1107 38-39 -0.7740756011072776 1107 40-41 -0.7615032346003829 1107 42-43 -0.795837016206562 1107 44-45 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0.6195645833875361 2311 20-21 0.7106142922933145 2311 22-23 0.7704313994820922 2311 24-25 0.7042807879413147 2311 26-27 0.8574463173538902 2311 28-29 0.8463280036789627 2311 30-31 0.8420859685036461 2311 32-33 0.841532660857709 2311 34-35 0.8874399010038658 2311 36-37 0.8907933995117645 2311 38-39 0.9098790576154343 2311 40-41 0.9803298014996074 2311 42-43 1.0207750660094845 2311 44-45 1.0550155719593732 2311 46-47 1.0734613502294579 2311 48-49 1.052524988457428 2311 50-51 0.9888862073599896 2311 52-53 0.903444097417875 2311 54-55 0.9310592160630122 2311 56-57 0.9027085746250378 2311 58-59 0.8371659326690022 2311 60-61 0.8338587901155563 2311 62-63 0.8401918737856633 2311 64-65 0.8465312406265895 2311 66-67 0.8058760018918818 2311 68-69 0.8561246166907139 2311 70-71 0.8799022082116963 2311 72-73 0.8791073264513116 2311 74-75 0.9133725676205486 2311 76-77 0.8896719209233268 2311 78-79 0.8413065720800397 2311 80-81 0.8728190333174304 2311 82-83 0.8462913951877198 2311 84-85 0.9773336350258575 2311 86-87 0.9925998330141681 2311 88-89 0.9741480966625282 2311 90-91 1.041807821603733 2311 92-93 1.0167454131901366 2311 94-95 1.0555011962364773 2311 96-97 0.9884717134068133 2311 98-99 1.029372212714275 2311 100 1.084600389505571 2312 1 0.47668219133004897 2312 2 0.5094758367830536 2312 3 0.5221015333442338 2312 4 0.4269348596677105 2312 5 0.40399449403729903 2312 6 0.4605883966971973 2312 7 0.4860526702309471 2312 8 0.48924155373337186 2312 9 0.5975744068759141 2312 10-11 0.602893115985971 2312 12-13 0.6212412323134302 2312 14-15 0.8088031039014538 2312 16-17 0.7366221245109728 2312 18-19 0.7078622121673916 2312 20-21 0.7347213541053463 2312 22-23 0.7853888383672682 2312 24-25 0.8055649357650196 2312 26-27 0.8489790783905846 2312 28-29 0.8003382307970099 2312 30-31 0.8384541157482772 2312 32-33 0.844043037631053 2312 34-35 0.8126533729546068 2312 36-37 0.8908967022146115 2312 38-39 0.8980787247366777 2312 40-41 0.9695184718020045 2312 42-43 0.9822873321682621 2312 44-45 0.9680469488960526 2312 46-47 0.9919279382933084 2312 48-49 0.9697440908748192 2312 50-51 0.9210402423304558 2312 52-53 0.8522729885606211 2312 54-55 0.8415075525161839 2312 56-57 0.7909103583876416 2312 58-59 0.8130327316976462 2312 60-61 0.7909960431438492 2312 62-63 0.7620727533947473 2312 64-65 0.7929755855269462 2312 66-67 0.8005771686757441 2312 68-69 0.8176248809054485 2312 70-71 0.8357081626147362 2312 72-73 0.8537510378152646 2312 74-75 0.8457844186797345 2312 76-77 0.8579201939833112 2312 78-79 0.8913326954756151 2312 80-81 0.9043087309489799 2312 82-83 0.8761236350949844 2312 84-85 0.8514384727164384 2312 86-87 0.8893425584245023 2312 88-89 0.9799037776089818 2312 90-91 0.9632321329307523 2312 92-93 1.039357899780562 2312 94-95 1.0369468284617724 2312 96-97 1.0777633611056316 2312 98-99 1.115337365775531 2312 100 1.1873417023121746 2313 1 0.48934584039381335 2313 2 0.48468490709136347 2313 3 0.5122497355625484 2313 4 0.4835193593246885 2313 5 0.4238807663167563 2313 6 0.40914315321991523 2313 7 0.41481200048009725 2313 8 0.41469341695569284 2313 9 0.5718061159582462 2313 10-11 0.5934365433449642 2313 12-13 0.6202437409179851 2313 14-15 0.8306233846744178 2313 16-17 0.8396437107767625 2313 18-19 0.7514207408109641 2313 20-21 0.7469633316841922 2313 22-23 0.7226385212573092 2313 24-25 0.7141593013151137 2313 26-27 0.7512278757501178 2313 28-29 0.776033097941415 2313 30-31 0.8503822047621767 2313 32-33 0.8630952794299773 2313 34-35 0.8232291112753458 2313 36-37 0.8614787946915712 2313 38-39 0.8968244294916587 2313 40-41 0.9013042415024515 2313 42-43 0.82275398072575 2313 44-45 0.9428471418418312 2313 46-47 0.9344800499002517 2313 48-49 1.0007505866877793 2313 50-51 0.9829477439907421 2313 52-53 0.8471911617428205 2313 54-55 0.8213995318604645 2313 56-57 0.8760163540692858 2313 58-59 0.8692650237249353 2313 60-61 0.8928951720040814 2313 62-63 0.8920019334610103 2313 64-65 0.9237069830910514 2313 66-67 0.9072399804595008 2313 68-69 0.8747038076283999 2313 70-71 0.8255621281526189 2313 72-73 0.8415311527809024 2313 74-75 0.8987427939626471 2313 76-77 0.8553196039766249 2313 78-79 0.90320582392199 2313 80-81 0.8991140007488134 2313 82-83 0.8250300646936211 2313 84-85 0.8756549259156259 2313 86-87 0.9026031915077866 2313 88-89 0.8789729155270898 2313 90-91 0.9312816573678084 2313 92-93 0.9440740636876868 2313 94-95 0.9798924630776717 2313 96-97 0.9874057896509143 2313 98-99 1.0555605743276075 2313 100 1.0826416101860517 2314 1 0.3764586665032752 2314 2 0.291728809925754 2314 3 0.3653006575558848 2314 4 0.2804743674273027 2314 5 0.262166890438543 2314 6 0.2973585276662263 2314 7 0.3817822719235835 2314 8 0.3613723592091418 2314 9 0.48276834266467716 2314 10-11 0.47641704346055747 2314 12-13 0.4970600601547943 2314 14-15 0.5992298854288691 2314 16-17 0.6315378816272883 2314 18-19 0.6231330696775572 2314 20-21 0.6544566147699626 2314 22-23 0.6556709330525763 2314 24-25 0.6616675787263233 2314 26-27 0.6885629646223705 2314 28-29 0.6814255779711687 2314 30-31 0.7070783135196521 2314 32-33 0.7461108622007373 2314 34-35 0.7365298704590302 2314 36-37 0.7982505348210012 2314 38-39 0.8469774812919368 2314 40-41 0.8772630792368403 2314 42-43 0.7540643825566562 2314 44-45 0.8881022801576037 2314 46-47 0.6858027932463315 2314 48-49 0.6289750910614273 2314 50-51 0.762037554827252 2314 52-53 0.7548709022598032 2314 54-55 0.856344269935903 2314 56-57 0.85961832824146 2314 58-59 0.8075989136458901 2314 60-61 0.614385445574797 2314 62-63 0.7178852981898487 2314 64-65 0.7757006121970491 2314 66-67 0.7968500996444092 2314 68-69 0.8231275051355524 2314 70-71 0.807283345593369 2314 72-73 0.8416763519584762 2314 74-75 0.8300929049608996 2314 76-77 0.8519174992801055 2314 78-79 0.7943812602266398 2314 80-81 0.8875726716452199 2314 82-83 0.8591741533699562 2314 84-85 0.9009079577506611 2314 86-87 0.9729971857002333 2314 88-89 0.8264588349205226 2314 90-91 0.8364461174279008 2314 92-93 1.020565275354631 2314 94-95 1.015503709361166 2314 96-97 1.0257623003810679 2314 98-99 1.0596443184857698 2314 100 1.1028726771531225 2315 1 0.3400443344592432 2315 2 0.38528631922683587 2315 3 0.414608321095038 2315 4 0.24286066517267813 2315 5 0.2560198434349843 2315 6 0.2459974943636638 2315 7 0.2471130261131549 2315 8 0.26970048526121104 2315 9 0.1102899133423918 2315 10-11 0.31497309407181007 2315 12-13 0.2837777343049126 2315 14-15 0.4980378900524016 2315 16-17 0.2809480603796004 2315 18-19 0.4826814832323194 2315 20-21 0.3740800365052692 2315 22-23 0.3741133029762125 2315 24-25 0.5103606013166626 2315 26-27 0.5654821034522612 2315 28-29 0.5297841151426326 2315 30-31 0.5673007709082469 2315 32-33 0.5000669988521622 2315 34-35 0.515708285723413 2315 36-37 0.6863273961914302 2315 38-39 0.6754014565860658 2315 40-41 0.7305433313978398 2315 42-43 0.7165268430639316 2315 44-45 0.7246017734276862 2315 46-47 0.7452062886654076 2315 48-49 0.6055364656599735 2315 50-51 0.554765610280576 2315 52-53 0.24807992385899524 2315 54-55 0.46263568437022684 2315 56-57 0.3631259320114921 2315 58-59 0.4335876392241005 2315 60-61 0.46668257415592507 2315 62-63 0.5265527412148145 2315 64-65 0.49669556046958263 2315 66-67 0.39667218261876513 2315 68-69 0.4264341734993664 2315 70-71 0.3822627769001983 2315 72-73 0.45520628070919855 2315 74-75 0.16373845136137533 2315 76-77 0.45204830255023865 2315 78-79 0.5463114131549496 2315 80-81 0.5538072248469241 2315 82-83 0.47258835948432676 2315 84-85 0.5175127528550618 2315 86-87 0.5205693216697647 2315 88-89 0.43783565663476764 2315 90-91 0.631966992860999 2315 92-93 0.6628883700797275 2315 94-95 0.6088689127750122 2315 96-97 0.6568899913274215 2315 98-99 0.772198655835016 2315 100 0.7772175268386192 2316 1 0.12443883485121177 2316 2 0.18446897616466273 2316 3 0.19446090104639424 2316 4 0.07545336050323215 2316 5 0.02563615733073732 2316 6 -0.12487408094438024 2316 7 -0.04128617789094591 2316 8 0.026813150842428968 2316 9 0.07313712635169622 2316 10-11 0.06225701937683681 2316 12-13 0.08485327351173311 2316 14-15 -0.02852173928687307 2316 16-17 0.13728052937394608 2316 18-19 0.20740304547477706 2316 20-21 0.2031083787835044 2316 22-23 0.12561335322847356 2316 24-25 0.0648340982713762 2316 26-27 0.04240507677835126 2316 28-29 0.10005533667472832 2316 30-31 -0.4278817051214219 2316 32-33 -0.2109253025006268 2316 34-35 -0.4575792376438912 2316 36-37 -0.10138600508297202 2316 38-39 -0.05379205484130267 2316 40-41 0.1687062894472433 2316 42-43 0.1711772681981003 2316 44-45 0.0035256110927690543 2316 46-47 -0.15431559117136828 2316 48-49 -0.0879703786987065 2316 50-51 0.09241440724131422 2316 52-53 -0.19339025644613628 2316 54-55 -0.12543790504865626 2316 56-57 -0.08121347179562122 2316 58-59 -0.5167509806476573 2316 60-61 -0.06086776856452758 2316 62-63 -0.060418996910577505 2316 64-65 -0.41433720400809193 2316 66-67 -0.600581289812844 2316 68-69 -0.6846953366415391 2316 70-71 -0.4786681161830799 2316 72-73 -0.6697628442983401 2316 74-75 -0.4646813560719423 2316 76-77 -0.1814614162380792 2316 78-79 -0.10464981219631042 2316 80-81 -0.11362066315082231 2316 82-83 0.10864954664386417 2316 84-85 0.13908601060898818 2316 86-87 0.18605508017747496 2316 88-89 0.1648146310865144 2316 90-91 -0.39045460355299966 2316 92-93 -0.4094801197391611 2316 94-95 -0.048867343230131866 2316 96-97 0.2270290292980306 2316 98-99 0.3250538489042256 2316 100 0.141097162610194 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 186.0 1 565.0 2 348.0 3 291.0 4 288.0 5 272.0 6 340.0 7 830.0 8 2341.0 9 3847.0 10 5879.0 11 9455.0 12 14060.0 13 18317.0 14 21304.0 15 24625.0 16 28841.0 17 34464.0 18 41285.0 19 49574.0 20 58672.0 21 70734.0 22 78758.0 23 80992.0 24 80913.0 25 83565.0 26 90305.0 27 103027.0 28 119860.0 29 141369.0 30 170477.0 31 211427.0 32 264693.0 33 343932.0 34 471908.0 35 657340.0 36 968819.0 37 1426517.0 38 1377156.0 39 331445.0 40 337.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.09728308916543 19.240089064309018 13.529691013338665 23.132936833186893 2 15.597309344135699 20.25016596442265 40.992589180474454 23.159935510967202 3 18.777693197273017 26.430977615883716 29.683628907908073 25.107700278935198 4 12.580998651071468 16.842494794998323 38.52378004262538 32.052726511304826 5 13.205958908652358 37.56558332306884 34.64965152620558 14.57880624207322 6 30.094885354197405 37.06231520697496 18.70781695989394 14.134982478933697 7 27.19022793937954 32.569830170912226 22.523004811158476 17.716937078549762 8 25.783600982327314 35.216339449864215 20.533822330888345 18.466237236920126 9 26.655197686926634 16.3128096536536 20.86738355912549 36.16460910029428 10-11 24.829067112911893 26.39956483780041 28.26695543906044 20.50441261022726 12-13 25.38558745612967 24.175944454639218 28.389032777700574 22.04943531153054 14-15 22.935420460180307 25.524102210608525 26.3582837612956 25.18219356791558 16-17 21.997848259078594 28.351921329730946 27.092224179075096 22.558006232115364 18-19 21.735124068365298 27.430149650455494 29.33788462993797 21.49684165124124 20-21 22.82228657210506 26.69523259887589 29.098981665924818 21.383499163094232 22-23 22.630522588604585 26.55462852742163 28.86843866811231 21.94641021586147 24-25 22.184174558135737 26.635940843808157 29.19032166372287 21.989562934333236 26-27 22.09025319348996 26.839303433543343 28.93174754746904 22.138695825497656 28-29 21.923757903169363 26.938174199389337 29.15563473101687 21.982433166424425 30-31 22.473935813068184 26.772223221900564 28.98498979583409 21.768851169197163 32-33 22.324214874740704 26.949193813215462 28.791925804942892 21.93466550710094 34-35 22.46071488361416 26.98502830670278 28.86230335440639 21.691953455276668 36-37 22.44173369051708 26.830107519311763 28.6817447596571 22.046414030514054 38-39 22.307971528591636 27.000782297599113 28.787718983755074 21.903527190054177 40-41 22.394686523418525 26.627197702895206 29.042815004109855 21.935300769576415 42-43 22.250823085374005 26.762046558308512 28.69301781089791 22.294112545419573 44-45 22.12186810232338 27.089349643691342 28.653420509618233 22.135361744367046 46-47 22.19415566539338 26.883818673071147 28.32641847940378 22.595607182131687 48-49 22.33201710819598 26.920932989920015 28.345871223526064 22.40117867835794 50-51 22.282503462964893 27.107344464469236 28.126762794690524 22.483389277875343 52-53 22.22722437723396 27.31547227708493 27.989998097390583 22.46730524829053 54-55 22.18865247218665 27.21678992988738 28.086805199429836 22.50775239849613 56-57 22.38319210395508 27.182795151481997 27.857164812050804 22.576847932512123 58-59 22.137429644829137 27.14473215562956 28.205050089205518 22.512788110335784 60-61 22.33630092152587 27.340897956122063 27.958243043909143 22.364558078442922 62-63 22.335040777098936 27.229504530777486 27.9721846828625 22.463270009261073 64-65 22.126144672695762 27.062990282049466 28.03678930196097 22.774075743293803 66-67 22.232523911654155 27.037134825983816 27.824533682123942 22.90580758023809 68-69 22.028449646979237 27.15022969165828 27.977968666174323 22.84335199518816 70-71 22.141597562555916 26.89554648656019 28.028948236811 22.933907714072895 72-73 22.25801830143102 27.145026051177222 27.77158946372504 22.825366183666723 74-75 22.29315994695614 27.315638867383036 27.634090240097404 22.757110945563426 76-77 22.17280791278872 27.214258996921338 27.688613349445962 22.924319740843984 78-79 22.11911376377402 27.112139622836455 27.740736505303037 23.028010108086487 80-81 22.154288832287385 27.362828601283468 27.564872904241454 22.918009662187693 82-83 22.515756217895692 27.18014102503867 27.72646850983759 22.577634247228048 84-85 22.515000887602447 27.221561955531048 27.443002178857512 22.82043497800899 86-87 22.184258310167728 27.391042662178428 27.67394515810611 22.750753869547733 88-89 22.3688097532287 27.07064799327735 27.63046915059136 22.930073102902593 90-91 22.594931574728538 27.081925200431677 27.57594031678775 22.74720290805203 92-93 22.58286077396425 27.18294800527613 27.48024683441145 22.75394438634817 94-95 22.354962672588996 27.01623683464205 27.581714403659362 23.047086089109595 96-97 22.48075122775358 26.99790253473345 27.524215651255346 22.997130586257626 98-99 22.399790879700294 27.086327135119614 27.388528993093885 23.125352992086203 100 22.70169918196067 26.929809810699595 27.191924945965173 23.17656606137456 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2765.0 1 1928.0 2 2706.0 3 3713.0 4 2404.0 5 1825.0 6 2513.0 7 4266.5 8 6864.0 9 8479.0 10 7612.0 11 6323.0 12 6411.5 13 6908.0 14 7496.5 15 8147.5 16 8679.5 17 9316.0 18 10085.5 19 10691.5 20 11465.5 21 13005.0 22 15047.5 23 17607.5 24 20900.0 25 24969.5 26 31212.0 27 39111.0 28 46986.5 29 57803.0 30 72847.0 31 87729.0 32 106283.0 33 129223.0 34 152173.0 35 173402.0 36 197078.0 37 223143.5 38 234973.0 39 236985.5 40 237648.5 41 236974.5 42 239270.0 43 247083.0 44 257248.0 45 269706.0 46 279204.5 47 284184.5 48 292171.0 49 294734.0 50 287878.5 51 278460.5 52 267961.5 53 256937.0 54 244641.0 55 225862.5 56 204293.5 57 179174.0 58 154723.5 59 135338.5 60 113393.0 61 91137.5 62 72881.0 63 57029.5 64 42726.0 65 31315.0 66 23322.5 67 17855.0 68 13629.5 69 10300.5 70 8081.5 71 6291.5 72 4857.0 73 3701.0 74 2826.0 75 2098.0 76 1495.5 77 976.5 78 622.5 79 452.0 80 298.0 81 199.0 82 155.5 83 103.0 84 78.0 85 65.5 86 57.5 87 49.5 88 33.0 89 26.5 90 26.5 91 17.5 92 12.5 93 11.0 94 11.0 95 11.5 96 7.5 97 4.5 98 3.5 99 3.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02553672457065959 2 0.1117553108131992 3 0.017078614948687017 4 0.017213944702638578 5 0.019325088864282934 6 0.04590385254036954 7 0.04605271526971626 8 0.03813592466354993 9 0.03350764707840654 10-11 0.036363104886784486 12-13 0.03619394269434503 14-15 0.031085244482673597 16-17 0.03763520457392916 18-19 0.03714801745970354 20-21 0.0476428398786471 22-23 0.047500743636997966 24-25 0.06640631026403106 26-27 0.0586519153626066 28-29 0.05132380918612957 30-31 0.04938182721692466 32-33 0.04411073330051135 34-35 0.04582942117569618 36-37 0.041370305782992244 38-39 0.04630984180222423 40-41 0.054964179567426566 42-43 0.05668286744261139 44-45 0.059524792275594166 46-47 0.06131791151545236 48-49 0.060580364356416345 50-51 0.05505214390749508 52-53 0.06445756180712858 54-55 0.050620094465581446 56-57 0.05536340234158366 58-59 0.052568842922483934 60-61 0.04896907146737241 62-63 0.056114482476014825 64-65 0.054192799969902665 66-67 0.05193955956660917 68-69 0.05737981567546193 70-71 0.05744748055243771 72-73 0.060505932991742985 74-75 0.051046383190528864 76-77 0.05204105688207284 78-79 0.06194719487132712 80-81 0.062136656526859305 82-83 0.05225081800069776 84-85 0.05812412932219551 86-87 0.05586412243120444 88-89 0.06451846019640678 90-91 0.06997901576835228 92-93 0.07499298315225761 94-95 0.07816646588242172 96-97 0.07369381751432262 98-99 0.08184743518990419 100 0.1271423038374917 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7389358.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.79420009873617 #Duplication Level Percentage of deduplicated Percentage of total 1 76.13914548775499 46.288184461293625 2 14.634138631367243 17.79341504455971 3 4.503921135173325 8.214368481619623 4 1.8417505194449983 4.478709984443621 5 0.884611518046125 2.6889624818871445 6 0.503548581050966 1.8367699917508258 7 0.3299945125061262 1.4043226697347633 8 0.21789105299569742 1.0597209820435807 9 0.1552373285393125 0.849377629261099 >10 0.7164200738579546 7.625598030364538 >50 0.04322636668532307 1.8227251790122665 >100 0.027312093696184796 3.1463143983247592 >500 0.0020651520346503874 0.8722066840152526 >1k 5.900374775911237E-4 0.5859145696533647 >5k 5.900374774104031E-5 0.19849138426258792 >10k+ 8.850562161156046E-5 1.134918027773384 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28493 0.3855950679341832 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22167 0.2999854655844256 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12618 0.17075908353607988 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3532975395156115E-5 2 0.0 0.0 0.0 0.0 1.3532975395156115E-5 3 0.0 0.0 0.0 0.0 1.3532975395156115E-5 4 0.0 0.0 0.0 0.0 1.3532975395156115E-5 5 0.0 8.119785237093669E-5 0.0 0.0 1.3532975395156115E-5 6 0.0 8.119785237093669E-5 0.0 0.0 2.706595079031223E-5 7 0.0 8.119785237093669E-5 0.0 1.3532975395156115E-5 2.706595079031223E-5 8 0.0 8.119785237093669E-5 0.0 1.3532975395156115E-5 2.706595079031223E-5 9 0.0 8.119785237093669E-5 0.0 2.706595079031223E-5 4.0598926185468346E-5 10-11 0.0 8.796434006851474E-5 0.0 3.383243848789029E-5 4.0598926185468346E-5 12-13 0.0 1.2179677855640504E-4 0.0 4.0598926185468346E-5 4.0598926185468346E-5 14-15 0.0 1.285632662539831E-4 0.0 5.413190158062446E-5 4.0598926185468346E-5 16-17 0.0 1.3532975395156115E-4 0.0 5.413190158062446E-5 4.0598926185468346E-5 18-19 0.0 1.3532975395156115E-4 0.0 9.473082776609281E-5 4.7365413883046404E-5 20-21 0.0 1.4886272934671726E-4 0.0 1.9622814322976367E-4 5.413190158062446E-5 22-23 0.0 1.4886272934671726E-4 0.0 4.73654138830464E-4 6.766487697578058E-5 24-25 0.0 1.4886272934671726E-4 0.0 0.0011570693962858478 1.0826380316124892E-4 26-27 0.0 1.556292170442953E-4 0.0 0.002598331275869974 1.420962416491392E-4 28-29 0.0 1.6239570474187339E-4 0.0 0.005981575124659003 1.6916219243945144E-4 30-31 0.0 1.6239570474187339E-4 0.0 0.0168688538300621 1.759286801370295E-4 32-33 0.0 1.6239570474187339E-4 0.0 0.04329198828910441 1.759286801370295E-4 34-35 0.0 1.6916219243945144E-4 0.0 0.08434426915031049 1.759286801370295E-4 36-37 0.0 1.8269516783460757E-4 0.0 0.14859883632651172 1.759286801370295E-4 38-39 0.0 1.9622814322976367E-4 0.0 0.24322140028944328 1.894616555321856E-4 40-41 0.0 2.0299463092734172E-4 0.0 0.3459705159771661 2.0299463092734172E-4 42-43 0.0 2.0299463092734172E-4 0.0 0.45074957797416226 2.232940940200759E-4 44-45 0.0 2.1652760632249785E-4 0.0 0.578196373758045 2.4359355711281008E-4 46-47 0.0 2.3682706941523203E-4 0.0 0.7255298768851097 2.571265325079662E-4 48-49 0.0 2.4359355711281008E-4 0.0 0.8814432864127032 2.706595079031223E-4 50-51 0.0 2.5036004481038813E-4 0.0 1.0446306702151933 2.706595079031223E-4 52-53 0.0 2.6389302020554423E-4 0.0 1.2064038580888894 2.909589709958565E-4 54-55 0.0 3.2479140948374677E-4 0.0 1.3887539350509206 3.1125843408859067E-4 56-57 0.0 3.51857360274059E-4 0.0 1.6001187112601662 3.1802492178616875E-4 58-59 0.0 3.6539033566921513E-4 0.0 1.8375547645681802 3.2479140948374677E-4 60-61 0.0 3.7892331106437123E-4 0.0 2.081994132643188 3.2479140948374677E-4 62-63 0.0 3.9245628645952733E-4 0.0 2.33209028443337 3.2479140948374677E-4 64-65 0.0 4.127557495522615E-4 0.0 2.58322847532898 3.2479140948374677E-4 66-67 0.0 4.465881880401518E-4 0.0 2.8690516821623744 3.2479140948374677E-4 68-69 0.0 4.601211634353079E-4 0.0 3.178401966720248 3.2479140948374677E-4 70-71 0.0 5.142530650159323E-4 0.0 3.5069690763392436 3.315578971813248E-4 72-73 0.0 5.548519912014007E-4 0.0 3.8299335341446445 3.9245628645952733E-4 74-75 0.0 5.548519912014007E-4 0.0 4.155394825910451 3.9245628645952733E-4 76-77 0.0 5.548519912014007E-4 0.0 4.5071236229182565 4.059892618546835E-4 78-79 0.0 5.548519912014007E-4 0.0 4.897049242978889 4.195222372498396E-4 80-81 0.0 5.683849665965568E-4 0.0 5.30134011642148 4.195222372498396E-4 82-83 0.0 5.683849665965568E-4 0.0 5.713466582617867 4.195222372498396E-4 84-85 0.0 5.683849665965568E-4 0.0 6.126628321431983 4.195222372498396E-4 86-87 0.0 5.95450917386869E-4 0.0 6.543281567898051 4.195222372498396E-4 88 0.0 6.089838927820252E-4 0.0 6.873993654117178 4.195222372498396E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12395 0.0 53.202404 1 GTATCAA 23510 0.0 42.994564 1 TACCTGG 5970 0.0 35.688892 2 ATCAACG 28830 0.0 34.603054 3 CAACGCA 29040 0.0 34.190548 5 TATCAAC 29795 0.0 33.686836 2 TCAACGC 29960 0.0 33.21951 4 AACGCAG 30685 0.0 32.618393 6 GTACCTG 7485 0.0 28.78084 1 ACGCAGA 34520 0.0 28.695564 7 ACCTGGG 7210 0.0 28.415827 3 CGCAGAG 35155 0.0 28.042055 8 GTACATG 30180 0.0 26.370045 1 TACATGG 31090 0.0 25.173393 2 GCAGAGT 38465 0.0 25.117773 9 GTGGTAT 5990 0.0 24.813616 1 ACATGGG 31290 0.0 24.328678 3 TGGTATC 6060 0.0 23.284012 2 CATGGGG 19710 0.0 22.863413 4 GAGTACT 22345 0.0 21.040644 12-13 >>END_MODULE