FastQCFastQC Report
Mon 6 Jun 2016
ERR1211302_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211302_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7389358
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253720.34335865172590097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213520.2889560906373734No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125920.1704072261758058No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA133250.051.664551
GTATCAA240000.040.2209931
TACCTGG65000.034.0680052
ATCAACG297450.032.036163
CAACGCA300550.031.6849675
TCAACGC306200.031.1821944
TATCAAC309250.030.9635932
AACGCAG316150.030.3438846
ACCTGGG75700.028.6345653
GTACCTG79350.028.6238611
ACGCAGA358800.026.4754687
CGCAGAG367450.025.813928
GTACATG304700.024.5034681
GTGGTAT67150.024.3898541
TACATGG313100.023.4953042
TGGTATC65750.023.4756812
GCAGAGT402550.023.3878239
ACATGGG310950.022.6935163
GAGTACT233450.021.24748812-13
CATGGGG194500.020.9144744
Produced by FastQC (version 0.11.5)