##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211302_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7389358 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1221286071131 33.0 31.0 34.0 30.0 34.0 2 32.25002402103133 34.0 31.0 34.0 30.0 34.0 3 32.31552862914478 34.0 31.0 34.0 30.0 34.0 4 35.9543072618758 37.0 35.0 37.0 35.0 37.0 5 35.85999676832547 37.0 35.0 37.0 35.0 37.0 6 35.87854641769962 37.0 35.0 37.0 35.0 37.0 7 35.79801763563221 37.0 35.0 37.0 33.0 37.0 8 35.78033720926771 37.0 35.0 37.0 33.0 37.0 9 37.402989948517856 39.0 37.0 39.0 34.0 39.0 10-11 37.35257650258656 39.0 37.0 39.0 34.0 39.0 12-13 37.28400146535058 39.0 37.0 39.0 34.0 39.0 14-15 38.520985449615516 40.0 38.0 41.0 34.0 41.0 16-17 38.50215654729409 40.0 38.0 41.0 34.0 41.0 18-19 38.518415605252855 40.0 38.0 41.0 34.0 41.0 20-21 38.49437934932913 40.0 38.0 41.0 34.0 41.0 22-23 38.3835313568513 40.0 38.0 41.0 34.0 41.0 24-25 38.2963019926765 40.0 38.0 41.0 34.0 41.0 26-27 38.196180710151005 40.0 38.0 41.0 33.5 41.0 28-29 38.09384495919673 40.0 37.5 41.0 33.0 41.0 30-31 38.09457973209581 40.0 37.0 41.0 33.0 41.0 32-33 38.02157514901836 40.0 37.0 41.0 33.0 41.0 34-35 37.885633704578936 40.0 37.0 41.0 33.0 41.0 36-37 37.73636403866209 40.0 37.0 41.0 33.0 41.0 38-39 37.62260226125193 40.0 37.0 41.0 33.0 41.0 40-41 37.5153993080319 40.0 36.5 41.0 32.0 41.0 42-43 37.41089056721842 40.0 36.0 41.0 32.0 41.0 44-45 37.25489582450871 40.0 36.0 41.0 31.0 41.0 46-47 37.01569480054965 39.5 36.0 41.0 31.0 41.0 48-49 35.473829661521336 39.0 35.0 41.0 24.0 41.0 50-51 36.20270725819483 39.0 35.0 40.0 29.0 41.0 52-53 36.30110342468182 39.0 35.0 40.0 29.5 41.0 54-55 36.139361768640796 39.0 35.0 40.0 29.0 41.0 56-57 35.93300750890673 38.0 35.0 40.0 28.5 41.0 58-59 35.70482435686564 38.0 34.5 40.0 28.0 41.0 60-61 35.45519441607783 38.0 34.0 40.0 28.0 41.0 62-63 35.14483754881006 37.0 34.0 40.0 27.5 41.0 64-65 34.81512663211066 37.0 34.0 39.5 27.0 41.0 66-67 34.48386348313345 36.0 33.5 39.0 26.0 40.5 68-69 34.122428227188344 36.0 33.0 39.0 26.0 40.0 70-71 33.761674018229996 35.0 33.0 38.5 26.0 40.0 72-73 33.381093864988 35.0 32.0 37.5 26.0 40.0 74-75 33.01754178103158 35.0 32.0 37.0 25.0 39.0 76-77 32.07514658242299 34.0 30.5 36.0 24.5 38.5 78-79 32.50611433361328 35.0 32.0 36.0 25.0 38.5 80-81 32.37309777114602 35.0 32.0 36.0 25.0 37.5 82-83 32.09885716729383 34.5 31.5 35.0 25.0 37.0 84-85 31.810700469513048 34.0 31.0 35.0 24.0 36.5 86-87 31.549543072618757 34.0 31.0 35.0 24.0 36.0 88-89 31.319845039311943 34.0 31.0 35.0 23.0 36.0 90-91 31.09855037474162 34.0 31.0 35.0 23.0 35.0 92-93 30.911049106025178 34.0 30.0 35.0 21.5 35.0 94-95 30.71709023977455 34.0 30.0 35.0 20.0 35.0 96-97 30.53007399289627 34.0 30.0 35.0 20.0 35.0 98-99 30.31448659003935 34.0 29.0 35.0 19.5 35.0 100 27.89089566373696 31.0 25.0 34.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.25992685159298645 1101 2 -0.2926284403997492 1101 3 -0.31722163363463096 1101 4 -0.20861900882206186 1101 5 -0.13596816010090862 1101 6 -0.15951228959276165 1101 7 -0.19145846415807455 1101 8 -0.20216107323259536 1101 9 -0.28415341306247655 1101 10-11 -0.3138611890891667 1101 12-13 -0.36178169089803447 1101 14-15 -0.4256010777291621 1101 16-17 -0.4458954632270107 1101 18-19 -0.5072032128781885 1101 20-21 -0.5329615374876226 1101 22-23 -0.5646146775545304 1101 24-25 -0.5110201906221334 1101 26-27 -0.5137303979090433 1101 28-29 -0.49831273450605096 1101 30-31 -0.5072493459733565 1101 32-33 -0.4015096535221261 1101 34-35 -0.3910531916664226 1101 36-37 -0.4451551276445329 1101 38-39 -0.4088043190817885 1101 40-41 -0.36970032911234796 1101 42-43 -0.4123665565711292 1101 44-45 -0.43781156543884947 1101 46-47 -0.4784543003554802 1101 48-49 0.7331068213712939 1101 50-51 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1103 28-29 -0.658301753631271 1103 30-31 -0.6772424471021097 1103 32-33 -0.6887249639610502 1103 34-35 -0.7389623374441072 1103 36-37 -0.6504363742383035 1103 38-39 -0.6898627899622412 1103 40-41 -0.7150040160003144 1103 42-43 -0.7695005460428348 1103 44-45 -0.7015935794178816 1103 46-47 -0.7506486161959671 1103 48-49 0.5289790022728482 1103 50-51 -0.3428392982843391 1103 52-53 -0.6880611899107194 1103 54-55 -0.7941831382362494 1103 56-57 -0.872859698737976 1103 58-59 -0.9257542599377686 1103 60-61 -0.9722352941382724 1103 62-63 -0.934922896690658 1103 64-65 -0.8910010354752202 1103 66-67 -0.8602651178885807 1103 68-69 -1.016377106255078 1103 70-71 -0.8941012719558685 1103 72-73 -0.9044915158144491 1103 74-75 -0.9985654864992419 1103 76-77 -0.9200842022727045 1103 78-79 -0.6643794712781563 1103 80-81 -0.7223292361269777 1103 82-83 -0.8030121503730676 1103 84-85 -0.858878912898362 1103 86-87 -0.7065625877422974 1103 88-89 -0.7240246996538744 1103 90-91 -0.8258616890405897 1103 92-93 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1105 70-71 -0.6992942703298084 1105 72-73 -0.6478165393854951 1105 74-75 -0.685610762041378 1105 76-77 -0.7265185053212306 1105 78-79 -0.6148180048173657 1105 80-81 -0.5912413495959541 1105 82-83 -0.5100965716184405 1105 84-85 -0.4309818657699296 1105 86-87 -0.4716508514100859 1105 88-89 -0.56765712795006 1105 90-91 -0.6930466582274235 1105 92-93 -0.661438645339171 1105 94-95 -0.579908579422078 1105 96-97 -0.5925476594487975 1105 98-99 -0.6319944294018072 1105 100 -0.6128775390286236 1106 1 -0.3264222198001612 1106 2 -0.24277166547796725 1106 3 -0.32365023069012366 1106 4 -0.15007945509849918 1106 5 -0.20172167096802696 1106 6 -0.3642589078265104 1106 7 -0.3124526088688455 1106 8 -0.3521517635184921 1106 9 -0.4997986985030991 1106 10-11 -0.44799012837747654 1106 12-13 -0.4207500649253788 1106 14-15 -0.5405791452862587 1106 16-17 -0.5725058421130385 1106 18-19 -0.579714080583031 1106 20-21 -0.5977550260988096 1106 22-23 -0.6158187143070606 1106 24-25 -0.6269447422791643 1106 26-27 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0.32798695855873206 2311 6 0.323898260000135 2311 7 0.3831611148363052 2311 8 0.22462879629608778 2311 9 0.423755850262026 2311 10-11 0.418153553030173 2311 12-13 0.48696562798879484 2311 14-15 0.4359577258456113 2311 16-17 0.4867535307277038 2311 18-19 0.5924965197572405 2311 20-21 0.6608790124304704 2311 22-23 0.6310706333164617 2311 24-25 0.6102105144909089 2311 26-27 0.669647197663835 2311 28-29 0.6966681877200429 2311 30-31 0.5988933515396369 2311 32-33 0.5697622255686952 2311 34-35 0.6643621124482593 2311 36-37 0.6884658191289148 2311 38-39 0.7199253156350167 2311 40-41 0.6506601892090274 2311 42-43 0.6560932473231347 2311 44-45 0.7360844806316322 2311 46-47 0.7571184107837254 2311 48-49 -6.22682603799246 2311 50-51 -1.1684171693469594 2311 52-53 0.22147421250875965 2311 54-55 0.7045475981092011 2311 56-57 0.8230378060225192 2311 58-59 0.8609140530381652 2311 60-61 0.7640829889339358 2311 62-63 0.637270429198793 2311 64-65 0.653578842288475 2311 66-67 0.815932628492817 2311 68-69 0.8380053178030948 2311 70-71 0.8737286353993525 2311 72-73 0.3566953369720238 2311 74-75 0.6224724870663394 2311 76-77 0.752724979108855 2311 78-79 0.6759618290598866 2311 80-81 0.7043413745114862 2311 82-83 0.7339788450527749 2311 84-85 0.7994108169705498 2311 86-87 0.8170975435734888 2311 88-89 0.8676918280525179 2311 90-91 0.9398154860876211 2311 92-93 0.8671999208970895 2311 94-95 0.8857642351687787 2311 96-97 0.931643680775391 2311 98-99 0.9428889026581366 2311 100 1.1735986984330395 2312 1 0.08673748745847831 2312 2 0.22874524147926678 2312 3 0.21718586215263969 2312 4 -0.020455711806292243 2312 5 0.16518581115281705 2312 6 0.1462248194410236 2312 7 0.2937089420315786 2312 8 0.350698491950574 2312 9 0.2962399597597667 2312 10-11 0.24766485593799814 2312 12-13 0.2779309500087592 2312 14-15 0.2876481412665015 2312 16-17 0.47911416795111705 2312 18-19 0.31565938469050536 2312 20-21 0.3785972653108445 2312 22-23 0.6150879776231619 2312 24-25 0.7055450371177798 2312 26-27 0.6427158025529849 2312 28-29 0.7681103866011512 2312 30-31 0.7634225567621584 2312 32-33 0.7130102786527388 2312 34-35 0.2833123122193655 2312 36-37 0.6529924075693643 2312 38-39 0.7389830843522347 2312 40-41 0.5216941987488397 2312 42-43 0.5508034811278861 2312 44-45 0.6660450044821644 2312 46-47 0.7481409709704963 2312 48-49 -4.877199749152055 2312 50-51 -0.8542459416310635 2312 52-53 0.4716662722748026 2312 54-55 0.7124630987481879 2312 56-57 0.8073302205559898 2312 58-59 0.8009976757055242 2312 60-61 0.8452976937243761 2312 62-63 0.6144633534774044 2312 64-65 0.8133706688198501 2312 66-67 0.8329009339882845 2312 68-69 0.7243498885004982 2312 70-71 0.8031252613440572 2312 72-73 0.84209746916504 2312 74-75 0.5413018706112425 2312 76-77 0.7176339459038417 2312 78-79 0.4674622443199894 2312 80-81 0.664562109680503 2312 82-83 0.6777069744654654 2312 84-85 0.6636511601203949 2312 86-87 0.7573768445034545 2312 88-89 0.8617462011152419 2312 90-91 0.6490160775090317 2312 92-93 0.8348752677231772 2312 94-95 0.8406997584324749 2312 96-97 0.914888751931656 2312 98-99 1.0455154123916373 2312 100 0.7421571275223009 2313 1 0.12918138006212843 2313 2 0.27907740334271125 2313 3 0.3223624526927509 2313 4 0.11122796515836342 2313 5 0.1519935728172399 2313 6 0.29287712724327264 2313 7 0.21184047123657734 2313 8 0.3060499308075393 2313 9 0.47619848401437537 2313 10-11 0.25638002360570766 2313 12-13 0.014372612679174779 2313 14-15 0.20793256466515686 2313 16-17 0.1369058939872403 2313 18-19 0.4632040667034545 2313 20-21 0.39360973659088927 2313 22-23 0.5241380643263724 2313 24-25 0.3088507809120671 2313 26-27 0.47280890775190443 2313 28-29 0.4352685215318104 2313 30-31 0.5774519273968437 2313 32-33 0.47355071532408743 2313 34-35 0.3883218125058363 2313 36-37 -0.29660655050093965 2313 38-39 0.17148182920592348 2313 40-41 0.35056055932252406 2313 42-43 0.5504509851205057 2313 44-45 0.5796094188440506 2313 46-47 0.5025497805291081 2313 48-49 -5.066230818401664 2313 50-51 -1.1205097188751907 2313 52-53 0.22403338889038338 2313 54-55 0.3188111990355367 2313 56-57 0.3920459919881125 2313 58-59 0.4243867170438378 2313 60-61 0.7509361879539398 2313 62-63 0.7994290569297604 2313 64-65 0.913999522090883 2313 66-67 0.960480708049289 2313 68-69 1.0249173735924302 2313 70-71 0.9910416593857363 2313 72-73 0.7121065762731575 2313 74-75 0.8504981788703887 2313 76-77 0.8653622682363888 2313 78-79 0.8285846201357572 2313 80-81 -0.12323123549084869 2313 82-83 0.35350662118169396 2313 84-85 0.7272690368925225 2313 86-87 0.8446466575629614 2313 88-89 0.8523700858496888 2313 90-91 0.8576536364101699 2313 92-93 0.8889037985457584 2313 94-95 0.8431083381329643 2313 96-97 0.8591636599919248 2313 98-99 1.0439978564502326 2313 100 1.1294503765443125 2314 1 0.07943667847320057 2314 2 0.2063232102116217 2314 3 0.2515008004138011 2314 4 0.057877474772276116 2314 5 0.198290533904121 2314 6 0.28253051711087807 2314 7 0.1044245041541032 2314 8 0.26790408198190363 2314 9 0.4394601460581029 2314 10-11 0.2932280354883119 2314 12-13 0.2120274412606662 2314 14-15 0.283215668829385 2314 16-17 0.48402438947591264 2314 18-19 0.5681291791003815 2314 20-21 0.6311968977637008 2314 22-23 0.3429844971091356 2314 24-25 0.39125284944018546 2314 26-27 0.5036702336162477 2314 28-29 0.6117141043087955 2314 30-31 0.5255732487923481 2314 32-33 0.5335740332221164 2314 34-35 0.3971649514055571 2314 36-37 0.46650356387764447 2314 38-39 0.5081301334370707 2314 40-41 0.30944413698157547 2314 42-43 0.3635852036967151 2314 44-45 0.556884184636381 2314 46-47 0.6153488708835084 2314 48-49 -6.374728441902743 2314 50-51 -1.4526027066525558 2314 52-53 -0.7874139300388876 2314 54-55 -0.3880072080099808 2314 56-57 -0.07470305748492478 2314 58-59 0.4155532599334393 2314 60-61 0.6587837761199467 2314 62-63 0.5325306036163511 2314 64-65 0.7100295251261244 2314 66-67 0.8328938670670709 2314 68-69 0.7805245300114905 2314 70-71 0.515693880868831 2314 72-73 0.6855221255172594 2314 74-75 0.6703611234607507 2314 76-77 0.6190609849221218 2314 78-79 0.4175691712503209 2314 80-81 0.4642850653574726 2314 82-83 0.5136191083593715 2314 84-85 0.4851422852580143 2314 86-87 0.6090059302309925 2314 88-89 0.5010358764988396 2314 90-91 0.6441451267743403 2314 92-93 0.6518001251849874 2314 94-95 0.7500320208062341 2314 96-97 0.751912269496799 2314 98-99 0.8812633401728291 2314 100 -0.44189042057261574 2315 1 0.09490844838944668 2315 2 0.19918667125478606 2315 3 0.28084787923345544 2315 4 0.0874854737856765 2315 5 0.18520008802683208 2315 6 0.22966021321997232 2315 7 0.31081028852312187 2315 8 0.21376295188223082 2315 9 0.4381279284583641 2315 10-11 0.25000217534012137 2315 12-13 0.459464642316739 2315 14-15 0.23928814143425114 2315 16-17 0.343695104284933 2315 18-19 0.44475832974032414 2315 20-21 0.34601859985642847 2315 22-23 0.3748668803191393 2315 24-25 0.46487353843117774 2315 26-27 0.5334656689832116 2315 28-29 0.585592786347668 2315 30-31 0.5180060612998929 2315 32-33 0.6277815981389665 2315 34-35 0.5842329973194893 2315 36-37 0.4859518641608176 2315 38-39 0.5764103411076249 2315 40-41 0.5942214443904348 2315 42-43 0.6365861271990312 2315 44-45 0.611945656598472 2315 46-47 0.4517760197029119 2315 48-49 -4.949500511104052 2315 50-51 -1.0001173441452096 2315 52-53 0.09287997873605036 2315 54-55 0.045520049414946584 2315 56-57 0.4617949111735058 2315 58-59 0.6120855429341319 2315 60-61 0.5321491794695206 2315 62-63 0.2921559558029969 2315 64-65 0.4659360253052043 2315 66-67 -0.3308487463657954 2315 68-69 0.42009020721604884 2315 70-71 0.4230380627093169 2315 72-73 0.5174763702938208 2315 74-75 0.6646490790883703 2315 76-77 0.6354862271631134 2315 78-79 0.5695938894372432 2315 80-81 0.4457791690439876 2315 82-83 0.4603274270366313 2315 84-85 0.5290937194862408 2315 86-87 0.5066695537510952 2315 88-89 0.479016977879386 2315 90-91 0.5469110856468262 2315 92-93 0.5886514904150424 2315 94-95 0.2511458147274581 2315 96-97 0.3376642011824771 2315 98-99 0.24189251213562102 2315 100 0.39090259798646443 2316 1 0.23554387824733425 2316 2 0.2706413738618707 2316 3 0.21768709208195958 2316 4 0.014274876587741403 2316 5 0.17644927896534313 2316 6 0.1913026180924291 2316 7 0.2744208133577928 2316 8 0.33249209216646136 2316 9 0.4912882331881079 2316 10-11 0.4161721888077281 2316 12-13 0.3178970932202745 2316 14-15 0.42024247217457145 2316 16-17 0.4196354849960997 2316 18-19 0.17983969821003853 2316 20-21 0.46100177539764786 2316 22-23 0.6127134702003403 2316 24-25 0.5777548827798142 2316 26-27 0.5880929981757532 2316 28-29 0.6093973251323632 2316 30-31 0.5538830035499842 2316 32-33 0.5355288694409026 2316 34-35 0.5267286856750317 2316 36-37 0.37299918169483703 2316 38-39 0.5246709694619653 2316 40-41 0.12433374815532972 2316 42-43 0.15380794070281922 2316 44-45 0.3801896414021897 2316 46-47 0.3654436512130914 2316 48-49 -5.1846822031983635 2316 50-51 -1.1021812449271309 2316 52-53 0.24016876846047097 2316 54-55 0.5114297159409062 2316 56-57 0.44112937015760423 2316 58-59 0.3027255758996148 2316 60-61 0.4286872405360782 2316 62-63 0.3080672505023898 2316 64-65 0.33075324776194037 2316 66-67 0.44207991551685666 2316 68-69 0.1854842050853449 2316 70-71 -0.2424120568910766 2316 72-73 -0.14007266586770584 2316 74-75 0.10868594415098443 2316 76-77 0.1831637081175117 2316 78-79 0.17421177120371567 2316 80-81 0.11012044223540585 2316 82-83 0.02908340203109816 2316 84-85 0.2600526533341565 2316 86-87 0.3345878837677816 2316 88-89 0.13167884042938383 2316 90-91 0.23455913887402602 2316 92-93 0.31209073542499155 2316 94-95 0.523403146533127 2316 96-97 0.4758191389701736 2316 98-99 0.4736129487273111 2316 100 -0.22624460095220655 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 6.0 11 22.0 12 31.0 13 180.0 14 668.0 15 1891.0 16 4528.0 17 9335.0 18 16057.0 19 23210.0 20 32399.0 21 45571.0 22 56905.0 23 59960.0 24 62233.0 25 66432.0 26 78255.0 27 95128.0 28 116750.0 29 144624.0 30 175806.0 31 218443.0 32 276410.0 33 359556.0 34 498187.0 35 702496.0 36 1094013.0 37 1662303.0 38 1401493.0 39 186449.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.29345771417711 19.9276613993603 14.330076711026255 23.448804175436337 2 15.344237420554844 20.689079776652925 40.51161307077597 23.455069732016263 3 18.420340725365268 26.306552143333796 30.28649463626924 24.986612495031704 4 12.64161713335343 17.223921798524565 38.39011915472951 31.744341913392493 5 13.103970931039033 37.68489002716757 34.736379845249736 14.474759196543662 6 30.29250451595511 37.32341492276188 18.32289579768521 14.061184763597803 7 27.203547914053672 32.627660651347696 22.349633716388816 17.819157718209812 8 25.860199002685867 35.53060841683757 20.25250620986796 18.356686370608603 9 26.441002928293354 16.232119085012542 20.77121740453271 36.555660582161394 10-11 24.880404272736588 26.518360611854842 28.127065072336656 20.474170043071922 12-13 25.329514774641883 24.171354719968605 28.39861422181924 22.100516283570272 14-15 22.79130930266595 25.594384187674436 26.30694352730237 25.30736298235724 16-17 21.949190715785992 28.445935992782033 27.056650605463535 22.54822268596844 18-19 21.695742830144567 27.464965661764236 29.299516229929328 21.539775278161873 20-21 22.724107239158826 26.701241637799683 29.121006940661786 21.4536441823797 22-23 22.54142739473405 26.565614387800483 28.85665688704281 22.036301330422656 24-25 22.12532686366846 26.650395518148905 29.2228045537112 22.001473064471437 26-27 22.03566779991864 26.818237426438092 29.00721768100432 22.13887709263895 28-29 21.877117083174657 26.928695759026507 29.213704313294343 21.980482844504497 30-31 22.353772838658085 26.720548218467265 29.081780621939863 21.843898320934784 32-33 22.30305333588048 26.92068578367393 28.839600271037096 21.93666060940849 34-35 22.39239284289445 26.976069777617006 28.964079456770857 21.667457922717688 36-37 22.373463051478364 26.81433780296942 28.739758893447824 22.072440252104393 38-39 22.25915750556285 26.94570556212114 28.893735888141702 21.901401044174303 40-41 22.339462776866366 26.605074492822745 29.120538625211363 21.93492410509953 42-43 22.20740892517763 26.709915148273645 28.797062988301402 22.28561293824732 44-45 22.059102075110324 27.047055625478478 28.731081816042803 22.1627604833684 46-47 22.117686993852516 26.844984108012053 28.432396335290726 22.604932562844695 48-49 22.358235208390862 26.977113494014983 28.307559784442898 22.35709151315126 50-51 22.244772922628297 27.036115853946992 28.212584292444948 22.506526930979764 52-53 22.13498326429181 27.282794655158188 28.037517331442146 22.544704749107854 54-55 22.14782595279091 27.159646766897716 28.116538555965885 22.575988724345493 56-57 22.31751942869316 27.12092901513017 27.892263900539383 22.66928765563729 58-59 22.046638842324004 27.115531564676708 28.242341651492666 22.595487941506622 60-61 22.23709043880893 27.316898025515123 28.013278674536124 22.432732861139826 62-63 22.236735916907833 27.233692800475097 27.95525718833907 22.57431409427801 64-65 22.003012359064268 27.032339038271715 28.04733010981812 22.9173184928459 66-67 22.054649359538054 27.012469460556467 27.867506194525426 23.065374985380053 68-69 21.84400604967188 27.083361676364216 28.0110773025286 23.061554971435303 70-71 21.919573270234434 26.82057626928213 28.100555747700966 23.15929471278247 72-73 21.947346364291263 27.04746090699084 27.843087330483705 23.162105398234186 74-75 21.939433098697823 27.176071914888606 27.71749891691591 23.166996069497657 76-77 21.77116394677525 27.04979911019578 27.812130727223867 23.3669062158051 78-79 21.65509240383554 26.946396984978012 27.86597923284708 23.532531378339367 80-81 21.593574313928794 27.198232978283787 27.69078581567463 23.51740689211279 82-83 21.908296012924268 26.955700872951077 27.902810021071232 23.233193093053423 84-85 21.8683209073742 26.96648696589002 27.655638869052613 23.509553257683173 86-87 21.459857018256233 27.151330140093794 27.878536515274643 23.510276326375333 88-89 21.541916515221594 26.83304607899006 27.856270800782713 23.768766605005638 90-91 21.687915800005996 26.76550901578638 27.877377156623872 23.66919802758375 92-93 21.604089253042158 26.881227209027408 27.792575113604652 23.722108424325782 94-95 21.231601233454025 26.66847299330768 27.9582957795363 24.141629993701994 96-97 21.270205212699075 26.63000630862571 27.97453596955349 24.125252509121726 98-99 21.106131342057346 26.688573138821187 27.863848264011903 24.341447255109564 100 21.056490517225622 26.57490406893905 27.93940284141406 24.429202572421264 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2469.0 1 1741.0 2 2218.5 3 3113.0 4 2075.0 5 1470.5 6 2130.5 7 3949.5 8 6311.5 9 7585.0 10 6788.0 11 5626.0 12 5668.0 13 6193.5 14 6815.5 15 7530.0 16 8307.0 17 8981.0 18 9738.0 19 10703.5 20 11762.5 21 13059.5 22 14839.0 23 17517.5 24 20842.0 25 25002.0 26 31238.5 27 39139.5 28 47215.0 29 57893.0 30 73039.5 31 88387.5 32 107247.0 33 130212.0 34 152930.5 35 173868.5 36 197466.5 37 223105.5 38 234749.5 39 237763.5 40 238168.5 41 236407.5 42 239695.5 43 248144.0 44 258444.5 45 271945.5 46 281938.5 47 287099.0 48 295020.5 49 297518.0 50 291046.5 51 281328.0 52 270841.0 53 259552.5 54 246704.5 55 228063.5 56 205518.5 57 179522.0 58 154988.0 59 134804.0 60 111637.0 61 88449.0 62 69761.5 63 54086.0 64 39873.5 65 28436.0 66 20837.0 67 16027.0 68 12318.5 69 9111.0 70 6950.5 71 5371.0 72 4173.0 73 3275.0 74 2457.5 75 1715.0 76 1095.0 77 615.0 78 362.0 79 231.5 80 141.5 81 77.0 82 55.5 83 40.0 84 20.5 85 10.0 86 8.0 87 6.5 88 2.0 89 1.0 90 2.0 91 1.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029150029001166273 2 0.0019081495307170123 3 6.766487697578058E-5 4 0.021233238394999944 5 4.4658818804015174E-4 6 2.7065950790312227E-5 7 2.7065950790312227E-5 8 0.004222288323288708 9 0.01020386344794771 10-11 0.001204434810168894 12-13 1.0826380316124891E-4 14-15 1.1503029085882699E-4 16-17 2.6389302020554423E-4 18-19 6.766487697578057E-6 20-21 6.766487697578058E-5 22-23 1.9622814322976367E-4 24-25 6.766487697578057E-6 26-27 5.4131901580624455E-5 28-29 8.661104252899913E-4 30-31 0.0025306663988941937 32-33 0.009493382239702015 34-35 0.01780262913232787 36-37 0.020915213473213776 38-39 0.02044832582208089 40-41 0.004384684028030581 42-43 0.004750074363699796 44-45 0.00901972810087155 46-47 0.011996982687805895 48-49 1.1971540694063003 50-51 0.017789096156932714 52-53 0.019115327745658012 54-55 0.009418950875028656 56-57 0.007361938614964927 58-59 0.008444576646577415 60-61 0.004973368457719872 62-63 0.0074566694427310185 64-65 0.019764910564625506 66-67 0.02963721611539189 68-69 0.02950865284913791 70-71 0.02724187947044926 72-73 0.023276717679668518 74-75 0.009946736915439744 76-77 0.014933638348554773 78-79 0.008424277183484681 80-81 0.0027268945421239574 82-83 0.01469681127913954 84-85 0.024352589223583427 86-87 0.02397366591251906 88-89 0.03337908381215256 90-91 0.021199405956512055 92-93 0.013749503001478614 94-95 0.006949182865412666 96-97 0.003633603893599417 98-99 0.01529902868422399 100 0.034414356429882 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7389358.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.704133532558856 #Duplication Level Percentage of deduplicated Percentage of total 1 76.1816244354221 46.24539502455111 2 14.584191273467747 17.706413890579313 3 4.481949825739858 8.162186421238236 4 1.7986743662283304 4.367478756364609 5 0.8982866617345554 2.726485673222549 6 0.5328232088405194 1.9406742731220827 7 0.3397178332277761 1.4435593698155333 8 0.2220572204687671 1.078383293056392 9 0.1477836071200483 0.8073968238484764 >10 0.739529525309441 7.886422613474794 >50 0.04323728451604601 1.801872712450037 >100 0.02727735275399267 3.1575865577832936 >500 0.0021652191101895345 0.9072607846154666 >1k 5.635450066297987E-4 0.607470896233464 >5k 2.9660263503086674E-5 0.09342797594212403 >10k+ 8.898079050926003E-5 1.0679849337026457 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25372 0.34335865172590097 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21352 0.2889560906373734 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12592 0.1704072261758058 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.3532975395156115E-5 0.0 0.0 0.0 3 0.0 1.3532975395156115E-5 0.0 0.0 0.0 4 0.0 1.3532975395156115E-5 0.0 0.0 0.0 5 0.0 1.3532975395156115E-5 0.0 0.0 0.0 6 0.0 1.3532975395156115E-5 0.0 0.0 0.0 7 0.0 1.3532975395156115E-5 0.0 0.0 0.0 8 0.0 1.3532975395156115E-5 0.0 0.0 0.0 9 0.0 1.3532975395156115E-5 0.0 1.3532975395156115E-5 2.706595079031223E-5 10-11 0.0 1.3532975395156115E-5 0.0 2.0299463092734173E-5 2.706595079031223E-5 12-13 0.0 1.3532975395156115E-5 0.0 2.706595079031223E-5 2.706595079031223E-5 14-15 6.766487697578058E-6 2.0299463092734173E-5 0.0 4.7365413883046404E-5 2.706595079031223E-5 16-17 1.3532975395156115E-5 2.706595079031223E-5 0.0 6.089838927820252E-5 2.706595079031223E-5 18-19 1.3532975395156115E-5 2.706595079031223E-5 0.0 1.3532975395156115E-4 3.383243848789029E-5 20-21 1.3532975395156115E-5 5.413190158062446E-5 0.0 2.232940940200759E-4 4.7365413883046404E-5 22-23 1.3532975395156115E-5 5.413190158062446E-5 0.0 4.8718711422562016E-4 6.089838927820252E-5 24-25 1.3532975395156115E-5 5.413190158062446E-5 0.0 0.0011706023716810039 6.766487697578058E-5 26-27 1.3532975395156115E-5 5.413190158062446E-5 0.0 0.002605097763567552 6.766487697578058E-5 28-29 1.3532975395156115E-5 6.766487697578058E-5 0.0 0.005968042149263847 6.766487697578058E-5 30-31 1.3532975395156115E-5 9.473082776609281E-5 0.0 0.016814721928481473 6.766487697578058E-5 32-33 1.3532975395156115E-5 9.473082776609281E-5 0.0 0.04322432341212863 9.473082776609281E-5 34-35 1.3532975395156115E-5 1.1503029085882697E-4 0.0 0.08418187344556861 1.0149731546367087E-4 36-37 1.3532975395156115E-5 1.6239570474187339E-4 0.0 0.14772595941352415 1.0826380316124892E-4 38-39 1.3532975395156115E-5 1.9622814322976367E-4 0.0 0.23848485890113863 1.2179677855640504E-4 40-41 1.3532975395156115E-5 2.0976111862491977E-4 0.0 0.336382402909698 1.3532975395156115E-4 42-43 1.3532975395156115E-5 2.1652760632249785E-4 0.0 0.43624222835055493 1.420962416491392E-4 44-45 1.3532975395156115E-5 2.1652760632249785E-4 0.0 0.5584652956319074 1.6239570474187339E-4 46-47 1.3532975395156115E-5 2.1652760632249785E-4 0.0 0.6992014732538334 1.8269516783460757E-4 48-49 1.3532975395156115E-5 2.1652760632249785E-4 0.0 0.8497287585741549 2.0299463092734172E-4 50-51 1.3532975395156115E-5 2.1652760632249785E-4 0.0 1.0126590158441369 2.0299463092734172E-4 52-53 1.3532975395156115E-5 2.1652760632249785E-4 0.0 1.1739720825543978 2.3006058171765395E-4 54-55 1.3532975395156115E-5 2.5036004481038813E-4 0.0 1.3563289260041265 2.6389302020554423E-4 56-57 1.3532975395156115E-5 2.706595079031223E-4 0.0 1.5669087896404532 2.706595079031223E-4 58-59 1.3532975395156115E-5 3.112584340885906E-4 0.0 1.8036884936418023 2.706595079031223E-4 60-61 1.3532975395156115E-5 3.3832438487890287E-4 0.0 2.047782770844233 2.706595079031223E-4 62-63 1.3532975395156115E-5 3.4509087257648095E-4 0.0 2.2971955073769603 2.841924832982784E-4 64-65 1.3532975395156115E-5 3.6539033566921513E-4 0.0 2.547460821359582 2.841924832982784E-4 66-67 1.3532975395156115E-5 3.9245628645952733E-4 0.0 2.832837440004937 2.841924832982784E-4 68-69 1.3532975395156115E-5 4.0598926185468344E-4 0.0 3.1426478457262457 2.841924832982784E-4 70-71 1.3532975395156115E-5 4.262887249474176E-4 0.0 3.471093158566685 2.841924832982784E-4 72-73 1.3532975395156115E-5 4.465881880401518E-4 0.0 3.7938546217411577 3.1125843408859067E-4 74-75 1.3532975395156115E-5 4.465881880401518E-4 0.0 4.119694836818029 3.1125843408859067E-4 76-77 1.3532975395156115E-5 4.465881880401518E-4 0.0 4.471240938657999 3.1802492178616875E-4 78-79 1.3532975395156115E-5 4.73654138830464E-4 0.0 4.8619379383161565 3.2479140948374677E-4 80-81 1.3532975395156115E-5 4.73654138830464E-4 0.0 5.266303243123422 3.4509087257648095E-4 82-83 1.3532975395156115E-5 4.73654138830464E-4 0.0 5.678977794823313 3.9245628645952733E-4 84-85 1.3532975395156115E-5 5.007200896207763E-4 0.0 6.092322228805263 3.9245628645952733E-4 86-87 1.3532975395156115E-5 5.548519912014007E-4 0.0 6.510200209544591 3.9245628645952733E-4 88 1.3532975395156115E-5 5.548519912014007E-4 0.0 6.841988167307633 3.9245628645952733E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13325 0.0 51.66455 1 GTATCAA 24000 0.0 40.220993 1 TACCTGG 6500 0.0 34.068005 2 ATCAACG 29745 0.0 32.03616 3 CAACGCA 30055 0.0 31.684967 5 TCAACGC 30620 0.0 31.182194 4 TATCAAC 30925 0.0 30.963593 2 AACGCAG 31615 0.0 30.343884 6 ACCTGGG 7570 0.0 28.634565 3 GTACCTG 7935 0.0 28.623861 1 ACGCAGA 35880 0.0 26.475468 7 CGCAGAG 36745 0.0 25.81392 8 GTACATG 30470 0.0 24.503468 1 GTGGTAT 6715 0.0 24.389854 1 TACATGG 31310 0.0 23.495304 2 TGGTATC 6575 0.0 23.475681 2 GCAGAGT 40255 0.0 23.387823 9 ACATGGG 31095 0.0 22.693516 3 GAGTACT 23345 0.0 21.247488 12-13 CATGGGG 19450 0.0 20.914474 4 >>END_MODULE