FastQCFastQC Report
Mon 6 Jun 2016
ERR1211301_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211301_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9393891
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323500.34437274181699573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262470.27940498777343703No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147400.15691048576143796No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA160050.051.4881171
GTATCAA286450.041.6745951
CAACGCA345900.033.621945
ATCAACG347800.033.4802253
TATCAAC365250.032.2579542
TCAACGC364850.031.9149654
AACGCAG370950.031.542326
ACGCAGA415800.027.9377987
CGCAGAG425700.027.1434218
GTGGTAT75900.026.151661
GTACATG373650.024.9749891
GTACTAG35300.024.5172481
GCAGAGT466800.024.1514849
TGGTATC79050.024.0975272
TACATGG381850.024.043982
ACATGGG385250.023.2486063
GAGTACT264450.021.6712412-13
CAGAGTA459750.020.70980510-11
CATGGGG252200.020.551234
GTACTTT281400.020.30727214-15
Produced by FastQC (version 0.11.5)