##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211301_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9393891 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.362819623944965 31.0 31.0 34.0 28.0 34.0 2 31.379875069872536 31.0 31.0 34.0 28.0 34.0 3 31.42712407457144 31.0 31.0 34.0 28.0 34.0 4 35.02678677025314 37.0 35.0 37.0 32.0 37.0 5 35.07616364720434 37.0 35.0 37.0 32.0 37.0 6 35.141089884904986 37.0 35.0 37.0 32.0 37.0 7 35.07582204221871 37.0 35.0 37.0 32.0 37.0 8 35.076537187838355 37.0 35.0 37.0 32.0 37.0 9 36.629426507077845 39.0 37.0 39.0 32.0 39.0 10-11 36.56603334017821 39.0 37.0 39.0 32.0 39.0 12-13 36.48588204823752 39.0 37.0 39.0 32.0 39.0 14-15 37.61492942594288 40.0 37.0 41.0 32.0 41.0 16-17 37.61458074188853 40.0 37.0 41.0 32.0 41.0 18-19 37.625634627866134 40.0 37.0 41.0 32.0 41.0 20-21 37.59734209179136 40.0 37.0 41.0 32.0 41.0 22-23 37.47760246526173 40.0 37.0 41.0 31.0 41.0 24-25 37.36846526109362 40.0 37.0 41.0 31.0 41.0 26-27 37.22435053802519 40.0 36.5 41.0 31.0 41.0 28-29 37.09192394291141 40.0 36.0 41.0 30.0 41.0 30-31 36.9162738848045 39.0 36.0 41.0 30.0 41.0 32-33 36.80040187819935 39.0 36.0 41.0 30.0 41.0 34-35 36.65714973699397 39.0 35.0 41.0 30.0 41.0 36-37 36.56324695485608 39.0 35.0 41.0 30.0 41.0 38-39 36.416680744965 39.0 35.0 41.0 29.0 41.0 40-41 36.25740361475346 39.0 35.0 40.5 28.5 41.0 42-43 36.107558678294225 39.0 35.0 40.0 28.0 41.0 44-45 35.9682377621797 39.0 35.0 40.0 27.5 41.0 46-47 35.797487058344615 39.0 35.0 40.0 27.0 41.0 48-49 35.55901681209629 38.5 34.0 40.0 26.0 41.0 50-51 34.89293685651664 37.5 33.5 39.5 25.0 40.5 52-53 35.128051145153805 38.0 34.0 40.0 26.0 40.5 54-55 35.485846759346046 38.0 34.0 40.0 26.0 41.0 56-57 35.3879203516413 38.0 34.0 40.0 26.0 41.0 58-59 35.16924903642165 38.0 34.0 40.0 26.0 41.0 60-61 34.91129378656831 38.0 34.0 40.0 26.0 41.0 62-63 34.64836429334767 37.0 33.5 40.0 25.0 41.0 64-65 34.37539582905529 37.0 33.0 40.0 25.0 41.0 66-67 34.15677938992479 36.0 33.0 39.5 24.5 41.0 68-69 33.82433796602494 36.0 33.0 39.0 24.0 41.0 70-71 33.488990877156226 35.0 32.0 39.0 23.5 40.5 72-73 33.13283388108293 35.0 32.0 38.0 23.0 40.0 74-75 32.761504311685115 35.0 32.0 37.0 22.5 39.5 76-77 32.40618381669534 35.0 31.0 37.0 22.0 39.0 78-79 32.03812259477995 35.0 31.0 36.0 21.0 39.0 80-81 31.696949538801334 35.0 31.0 36.0 20.0 38.0 82-83 31.376994155031177 34.5 30.5 35.5 20.0 37.0 84-85 31.071436000268687 34.0 30.0 35.0 19.5 37.0 86-87 30.81768795273439 34.0 30.0 35.0 19.0 36.0 88-89 30.55188467696719 34.0 29.5 35.0 18.0 36.0 90-91 30.291020089545427 34.0 29.0 35.0 18.0 36.0 92-93 30.051648832203824 34.0 29.0 35.0 17.5 35.0 94-95 29.833959165589636 34.0 29.0 35.0 16.5 35.0 96-97 29.632966520475915 34.0 29.0 35.0 15.0 35.0 98-99 29.398005842307516 34.0 27.5 35.0 14.0 35.0 100 27.724041188044442 32.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.35095162849664874 1101 2 -0.331674303699117 1101 3 -0.3460984323040712 1101 4 -0.22238600672090314 1101 5 -0.2742772972284726 1101 6 -0.26831730395991116 1101 7 -0.3157252319101289 1101 8 -0.4584019803611028 1101 9 -0.4757255046676363 1101 10-11 -0.49941219313632246 1101 12-13 -0.4494122739121096 1101 14-15 -0.49587562474896885 1101 16-17 -0.5420761307474109 1101 18-19 -0.5837229697025919 1101 20-21 -0.6308346652639898 1101 22-23 -0.6874857599052646 1101 24-25 -0.6072379611989831 1101 26-27 -0.6389213163557628 1101 28-29 -0.7075570127381141 1101 30-31 -0.8245688117384091 1101 32-33 -0.7402436665485439 1101 34-35 -0.655452617254376 1101 36-37 -0.8088625018264182 1101 38-39 -0.8475901107607413 1101 40-41 -0.8622029187963776 1101 42-43 -0.8560706486121035 1101 44-45 -0.901837406851989 1101 46-47 -0.8378210910792845 1101 48-49 -0.7758390648434812 1101 50-51 -0.7519156103089344 1101 52-53 -0.608326106427306 1101 54-55 -0.5728577685585634 1101 56-57 -0.5193318461749854 1101 58-59 -0.6873169374878927 1101 60-61 -0.6681764371860837 1101 62-63 -0.647595101442171 1101 64-65 -0.671512730517847 1101 66-67 -0.7911085882309266 1101 68-69 -0.6920741325921185 1101 70-71 -0.6863188525361394 1101 72-73 -0.7807951562045616 1101 74-75 -0.7689318525350615 1101 76-77 -0.7324223127816438 1101 78-79 -0.7665200270277204 1101 80-81 -0.8483161975257794 1101 82-83 -0.9195846954412303 1101 84-85 -0.8217333590837619 1101 86-87 -0.9236074369257885 1101 88-89 -0.9832647863496504 1101 90-91 -0.9391649396302704 1101 92-93 -0.9765504110788328 1101 94-95 -1.0395192958805772 1101 96-97 -1.0088945164786658 1101 98-99 -0.9935369082499186 1101 100 -1.0284513902147872 1102 1 -0.4023606918354048 1102 2 -0.42575066683859575 1102 3 -0.4674430981599471 1102 4 -0.33549642616603137 1102 5 -0.30329274697727016 1102 6 -0.3334062982474819 1102 7 -0.34792361267802363 1102 8 -0.35928367930380034 1102 9 -0.4701891883907976 1102 10-11 -0.4503195719304145 1102 12-13 -0.48777057296614856 1102 14-15 -0.5481728688227676 1102 16-17 -0.632070536208353 1102 18-19 -0.6040258445887474 1102 20-21 -0.5954289308253919 1102 22-23 -0.6600884911615097 1102 24-25 -0.7162372085279074 1102 26-27 -0.6893253154464603 1102 28-29 -0.7498644133373986 1102 30-31 -0.6868997157907799 1102 32-33 -0.7233387347598779 1102 34-35 -0.7333259679782884 1102 36-37 -0.7392824704458931 1102 38-39 -0.9722932003601343 1102 40-41 -1.0091696254667326 1102 42-43 -0.8419803243720381 1102 44-45 -0.9389463893795948 1102 46-47 -0.9297309486179373 1102 48-49 -0.896640919233775 1102 50-51 -0.7963903666646459 1102 52-53 -0.7632315917960781 1102 54-55 -0.7339923439640685 1102 56-57 -0.6691091930011694 1102 58-59 -0.6731213742902113 1102 60-61 -0.748036575770719 1102 62-63 -0.7547617739826435 1102 64-65 -0.8321645978820271 1102 66-67 -0.7241858856141192 1102 68-69 -0.7441338483454345 1102 70-71 -0.7673326522617359 1102 72-73 -0.806758804994054 1102 74-75 -0.8309330817054743 1102 76-77 -0.7599305397115614 1102 78-79 -0.8142624981013071 1102 80-81 -0.8652513484814683 1102 82-83 -0.9629665638997942 1102 84-85 -0.8812247743671442 1102 86-87 -0.9421154787794102 1102 88-89 -1.0475032386089325 1102 90-91 -1.090786505800299 1102 92-93 -1.044598204456193 1102 94-95 -1.0547595199671633 1102 96-97 -1.1338898277417542 1102 98-99 -1.1718746397215263 1102 100 -1.1927470502168553 1103 1 -0.47197291627944793 1103 2 -0.44491906442998896 1103 3 -0.4547105665124036 1103 4 -0.3267783756472369 1103 5 -0.3701696799776997 1103 6 -0.38462383634799835 1103 7 -0.4651218494211804 1103 8 -0.414890395991101 1103 9 -0.5094784628979738 1103 10-11 -0.5481592648990627 1103 12-13 -0.6535413818553764 1103 14-15 -0.6737765943860694 1103 16-17 -0.7134117459888998 1103 18-19 -0.7052596428912281 1103 20-21 -0.767823263721354 1103 22-23 -0.7405753208013905 1103 24-25 -0.8066910308992519 1103 26-27 -0.7649621935307209 1103 28-29 -0.7904377461758045 1103 30-31 -0.7906939512062294 1103 32-33 -0.8646039533136687 1103 34-35 -0.9562466122750024 1103 36-37 -1.054450024497278 1103 38-39 -1.0638555004699342 1103 40-41 -1.072009455618229 1103 42-43 -1.0012480746217847 1103 44-45 -1.0801723763294717 1103 46-47 -1.0156940367490321 1103 48-49 -1.0009853789028256 1103 50-51 -1.0414967452365786 1103 52-53 -0.9049919193779488 1103 54-55 -0.88558743077391 1103 56-57 -0.9063342153673446 1103 58-59 -0.9593303962948738 1103 60-61 -0.8812223568494915 1103 62-63 -0.895625638568653 1103 64-65 -0.8374229239392932 1103 66-67 -0.8393793699131393 1103 68-69 -0.8798835633547952 1103 70-71 -0.8090010135400263 1103 72-73 -1.0429719155854826 1103 74-75 -0.983435579240215 1103 76-77 -0.9525090553716566 1103 78-79 -0.9506150106723688 1103 80-81 -0.8824217048287046 1103 82-83 -1.0044185259641658 1103 84-85 -0.9205337924446191 1103 86-87 -1.0834097747167029 1103 88-89 -1.106224839742545 1103 90-91 -0.9579583415703787 1103 92-93 -1.050584594841986 1103 94-95 -1.3048196517810524 1103 96-97 -1.3175149705060605 1103 98-99 -1.351676873165843 1103 100 -1.2855907860446543 1104 1 -0.3990999927479635 1104 2 -0.3911399440356895 1104 3 -0.4472549807226578 1104 4 -0.28185611375680253 1104 5 -0.28767832683584516 1104 6 -0.29626008161083206 1104 7 -0.30752735825299027 1104 8 -0.32289713945446863 1104 9 -0.4036586546672112 1104 10-11 -0.379546670393907 1104 12-13 -0.43170801341094034 1104 14-15 -0.5388322678003306 1104 16-17 -0.5274473586389732 1104 18-19 -0.5787625341937925 1104 20-21 -0.5621386099108108 1104 22-23 -0.5151973501324179 1104 24-25 -0.6389843694239659 1104 26-27 -0.70361862294628 1104 28-29 -0.5973306016197384 1104 30-31 -0.6374946636617622 1104 32-33 -0.7157926672866495 1104 34-35 -0.6148026479265667 1104 36-37 -0.6703119207445738 1104 38-39 -0.6609333374502242 1104 40-41 -0.7035709508794312 1104 42-43 -0.6353004992931517 1104 44-45 -0.7229716291759019 1104 46-47 -0.7288970099597307 1104 48-49 -0.6554583466196675 1104 50-51 -0.5452478900117086 1104 52-53 -0.5100672590062345 1104 54-55 -0.49595823065009625 1104 56-57 -0.49931296122547764 1104 58-59 -0.632501884108791 1104 60-61 -0.5035711522589068 1104 62-63 -0.5449264413003618 1104 64-65 -0.5697807882729222 1104 66-67 -0.5873838867863839 1104 68-69 -0.45313712586567334 1104 70-71 -0.5442007250826748 1104 72-73 -0.5459102816787436 1104 74-75 -0.5943044237545365 1104 76-77 -0.5387880701661558 1104 78-79 -0.4769142380016227 1104 80-81 -0.6139417263510758 1104 82-83 -0.5670647317200164 1104 84-85 -0.6157830672704669 1104 86-87 -0.6416741483819628 1104 88-89 -0.6403828175776489 1104 90-91 -0.6127638853667143 1104 92-93 -0.6527477251478757 1104 94-95 -0.6653473389828477 1104 96-97 -0.678626474701602 1104 98-99 -0.7179560318692175 1104 100 -0.7939121169779071 1105 1 -0.32975403597106023 1105 2 -0.29875734866080705 1105 3 -0.2936273961235649 1105 4 -0.18241960376302302 1105 5 -0.20062729674928192 1105 6 -0.2021221693567341 1105 7 -0.2432884746777475 1105 8 -0.21114744231177696 1105 9 -0.3351590871483836 1105 10-11 -0.340208027618921 1105 12-13 -0.37717083081636105 1105 14-15 -0.5010816559491502 1105 16-17 -0.5751290279372157 1105 18-19 -0.5292862228066824 1105 20-21 -0.552549642052341 1105 22-23 -0.5191578589057784 1105 24-25 -0.5056709409531024 1105 26-27 -0.5833481347203389 1105 28-29 -0.6311052014898948 1105 30-31 -0.5463598682125266 1105 32-33 -0.5567186949650633 1105 34-35 -0.5865847335067613 1105 36-37 -0.668172247367103 1105 38-39 -0.6329183298324992 1105 40-41 -0.7245452142344888 1105 42-43 -0.752500705101923 1105 44-45 -0.8187907454141055 1105 46-47 -0.7897480036512547 1105 48-49 -0.7946060249973428 1105 50-51 -0.8406106825671387 1105 52-53 -0.5593467777128325 1105 54-55 -0.5905629887723052 1105 56-57 -0.6617827064177249 1105 58-59 -0.6705428526456387 1105 60-61 -0.7239818256283925 1105 62-63 -0.6339385726789075 1105 64-65 -0.6182306514441365 1105 66-67 -0.6010932726923457 1105 68-69 -0.6092782873716374 1105 70-71 -0.6204086169062535 1105 72-73 -0.6359974153456065 1105 74-75 -0.7298941576342557 1105 76-77 -0.6865224683154203 1105 78-79 -0.6827524281201143 1105 80-81 -0.7004985764725404 1105 82-83 -0.6815988857094624 1105 84-85 -0.6713070524246589 1105 86-87 -0.6295749875329726 1105 88-89 -0.8198879795433598 1105 90-91 -0.829816156350784 1105 92-93 -0.8008832583806083 1105 94-95 -0.7709707375748778 1105 96-97 -0.9416176275676769 1105 98-99 -0.9059278678923768 1105 100 -0.9112156540049341 1106 1 -0.5293675038551164 1106 2 -0.48707210753645214 1106 3 -0.5063924051822681 1106 4 -0.4989263848220489 1106 5 -0.38664938207004695 1106 6 -0.3587391597201872 1106 7 -0.44636173943293755 1106 8 -0.46978650006499834 1106 9 -0.5415789656592622 1106 10-11 -0.6027142490179571 1106 12-13 -0.5793607911371836 1106 14-15 -0.6335513996634745 1106 16-17 -0.6560601916835225 1106 18-19 -0.7619863393683204 1106 20-21 -0.6895739424596243 1106 22-23 -0.6403657623340635 1106 24-25 -0.7218103340261806 1106 26-27 -0.7003162300488412 1106 28-29 -0.696708834979411 1106 30-31 -0.7103663559933082 1106 32-33 -0.7527039969129916 1106 34-35 -0.7697678132362995 1106 36-37 -0.8202051379397659 1106 38-39 -0.7721700443198998 1106 40-41 -0.8077953781967864 1106 42-43 -0.749908909264505 1106 44-45 -0.7438721212628892 1106 46-47 -0.7959192946590505 1106 48-49 -0.811163069844703 1106 50-51 -0.7492571748478909 1106 52-53 -0.6003769993521786 1106 54-55 -0.7233941004174085 1106 56-57 -0.6718428846822704 1106 58-59 -0.6705175327038972 1106 60-61 -0.6945516260026565 1106 62-63 -0.5487714377632145 1106 64-65 -0.6169938365552952 1106 66-67 -0.6242521551938438 1106 68-69 -0.5859526630182117 1106 70-71 -0.6172413384919011 1106 72-73 -0.5129478043166387 1106 74-75 -0.5304214468457076 1106 76-77 -0.5094390442296088 1106 78-79 -0.5093172093841858 1106 80-81 -0.6212750873569597 1106 82-83 -0.5635634842394062 1106 84-85 -0.5426950446509089 1106 86-87 -0.62216354489982 1106 88-89 -0.6788817051617535 1106 90-91 -0.5866042560820013 1106 92-93 -0.6380331365814911 1106 94-95 -0.6926887716644501 1106 96-97 -0.7025399058442581 1106 98-99 -0.6787306883173443 1106 100 -0.7715115742867376 1107 1 -0.4802361110551452 1107 2 -0.4733101219127889 1107 3 -0.45255684537512764 1107 4 -0.32295528691969366 1107 5 -0.33047718647364377 1107 6 -0.3940069174311276 1107 7 -0.36771204291918025 1107 8 -0.34759417847533314 1107 9 -0.4620844268894544 1107 10-11 -0.45701258429014757 1107 12-13 -0.5115895903635703 1107 14-15 -0.6465967315194376 1107 16-17 -0.6451467695876758 1107 18-19 -0.6186920895033197 1107 20-21 -0.579476480151051 1107 22-23 -0.6095882821271701 1107 24-25 -0.6460639064791209 1107 26-27 -0.668768967954648 1107 28-29 -0.6748996667784866 1107 30-31 -0.6984863876879075 1107 32-33 -0.6791628119546331 1107 34-35 -0.6894039712430313 1107 36-37 -0.7009684873687689 1107 38-39 -0.745284061170274 1107 40-41 -0.7330037295566214 1107 42-43 -0.8152165626380281 1107 44-45 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16-17 0.7847230554339291 2311 18-19 0.6821510181048964 2311 20-21 0.7483006725457173 2311 22-23 0.7941953346500341 2311 24-25 0.8026377266711933 2311 26-27 0.9035055438043145 2311 28-29 0.8704629727407394 2311 30-31 0.8419318783497118 2311 32-33 0.8308785575104807 2311 34-35 0.9043788754454667 2311 36-37 0.8695941857111649 2311 38-39 0.9495875676664625 2311 40-41 0.9574972293891548 2311 42-43 1.0055498413863262 2311 44-45 0.9710645599882923 2311 46-47 0.9703599305514743 2311 48-49 0.9907991992813052 2311 50-51 0.9617579290786438 2311 52-53 0.8690600374220168 2311 54-55 0.8345125982525374 2311 56-57 0.8137641712722044 2311 58-59 0.8083738446437181 2311 60-61 0.8282310797848638 2311 62-63 0.8593277127163432 2311 64-65 0.8544945342939911 2311 66-67 0.8277693938786683 2311 68-69 0.8459601718534557 2311 70-71 0.8075913971653179 2311 72-73 0.7073665858284457 2311 74-75 0.7991823230616362 2311 76-77 0.8758856734861453 2311 78-79 0.820551704846185 2311 80-81 0.8591100497912443 2311 82-83 0.82309575212631 2311 84-85 0.856625697228548 2311 86-87 0.8719922492952144 2311 88-89 0.8900062600796481 2311 90-91 0.8913898607659938 2311 92-93 0.8265622758251894 2311 94-95 0.9968947811536708 2311 96-97 0.9727198459281894 2311 98-99 0.9812544786751509 2311 100 1.0615617366232932 2312 1 0.45721960046018495 2312 2 0.4614651486539145 2312 3 0.5123573775952686 2312 4 0.39983075211877406 2312 5 0.40852007209850427 2312 6 0.407306547499374 2312 7 0.4169780490637862 2312 8 0.4661340237843916 2312 9 0.5381557764628297 2312 10-11 0.5747822640238169 2312 12-13 0.5769783991159656 2312 14-15 0.7599020034941901 2312 16-17 0.7544125000976791 2312 18-19 0.660886018217191 2312 20-21 0.6752656004681796 2312 22-23 0.7554796741617906 2312 24-25 0.7444923860808146 2312 26-27 0.8040491510823813 2312 28-29 0.8275806152868839 2312 30-31 0.899520831993506 2312 32-33 0.8088032856744221 2312 34-35 0.8532853842009374 2312 36-37 0.8543107602927549 2312 38-39 0.9149271800673802 2312 40-41 0.9620674277513785 2312 42-43 0.9548106219083365 2312 44-45 0.9358264845044033 2312 46-47 0.9373515753870336 2312 48-49 0.9565900758376742 2312 50-51 1.0207565146838178 2312 52-53 0.9122639393138456 2312 54-55 0.8662740636647825 2312 56-57 0.8027516577603322 2312 58-59 0.8254133474952994 2312 60-61 0.8552517693866193 2312 62-63 0.7731314290829374 2312 64-65 0.7864396645081939 2312 66-67 0.8339801535635019 2312 68-69 0.8092620809586535 2312 70-71 0.8191368902942031 2312 72-73 0.8539558950722466 2312 74-75 0.8806577365062296 2312 76-77 0.8918326524232256 2312 78-79 0.8798139829185168 2312 80-81 0.8981299921473216 2312 82-83 0.8793867349101028 2312 84-85 0.8793148859858704 2312 86-87 0.926603253063849 2312 88-89 0.9794700888549173 2312 90-91 0.9909031707755389 2312 92-93 1.0361251726121878 2312 94-95 1.014263994622766 2312 96-97 1.0481565663682986 2312 98-99 1.0929272032070045 2312 100 1.1499964434159011 2313 1 0.46086260752892727 2313 2 0.4561160192092899 2313 3 0.498817205967498 2313 4 0.3943695284052495 2313 5 0.39951320125252465 2313 6 0.40203868356617534 2313 7 0.40517607532059685 2313 8 0.4172051587621013 2313 9 0.5005687206420362 2313 10-11 0.5577305609106489 2313 12-13 0.5366308094090968 2313 14-15 0.6816306217957475 2313 16-17 0.6739154407871126 2313 18-19 0.6985527527751287 2313 20-21 0.6622806864979793 2313 22-23 0.6371863315072872 2313 24-25 0.6463444463542132 2313 26-27 0.7126643833674393 2313 28-29 0.7175527231952401 2313 30-31 0.8147224809693441 2313 32-33 0.7367324511637534 2313 34-35 0.7753079524381832 2313 36-37 0.8225631471696317 2313 38-39 0.8589813158188662 2313 40-41 0.889494265839538 2313 42-43 0.6718085220392567 2313 44-45 0.8413701184736624 2313 46-47 0.8724713785454128 2313 48-49 0.9273795447794555 2313 50-51 0.8285008459043652 2313 52-53 0.7323976341143563 2313 54-55 0.6788058062342301 2313 56-57 0.6772794649028739 2313 58-59 0.7225184024934563 2313 60-61 0.7394775442453394 2313 62-63 0.7397911479450698 2313 64-65 0.7289621097137626 2313 66-67 0.7696292026153486 2313 68-69 0.7250329852080526 2313 70-71 0.7878908111851146 2313 72-73 0.7530447579455952 2313 74-75 0.7347353667177714 2313 76-77 0.7563681452203426 2313 78-79 0.7389027596526923 2313 80-81 0.7541581646446467 2313 82-83 0.7405329345120215 2313 84-85 0.775649505399759 2313 86-87 0.8092651485269293 2313 88-89 0.8448043524913444 2313 90-91 0.8920381790851373 2313 92-93 0.8719908276875792 2313 94-95 0.8804402120271604 2313 96-97 0.9568817394428706 2313 98-99 0.919783040569893 2313 100 1.0579195587615864 2314 1 0.31533860202120323 2314 2 0.24006507345158568 2314 3 0.2906171899496499 2314 4 0.2453827605771508 2314 5 0.20858261495916963 2314 6 0.2162992672259847 2314 7 0.3140061905521634 2314 8 0.2902509861658231 2314 9 0.3365288311014467 2314 10-11 0.40424662273792933 2314 12-13 0.42048087980371207 2314 14-15 0.4713375177385899 2314 16-17 0.5106309564895994 2314 18-19 0.5397553296631656 2314 20-21 0.5417115113157749 2314 22-23 0.5306349608562897 2314 24-25 0.6192589564489381 2314 26-27 0.6020396096453666 2314 28-29 0.5714011699521464 2314 30-31 0.5698750909865637 2314 32-33 0.662433664391294 2314 34-35 0.6548322160797824 2314 36-37 0.6506128823638804 2314 38-39 0.7001425344022536 2314 40-41 0.7343588438950022 2314 42-43 0.6055112180769129 2314 44-45 0.7356880395821506 2314 46-47 0.5664918476574385 2314 48-49 0.5032747070614008 2314 50-51 0.6729257798015595 2314 52-53 0.6110814279429633 2314 54-55 0.5753996852318366 2314 56-57 0.5441350296588041 2314 58-59 0.5618458137835418 2314 60-61 0.3652999372637922 2314 62-63 0.5422889743507611 2314 64-65 0.5311205703150392 2314 66-67 0.589980446181201 2314 68-69 0.6283260379353734 2314 70-71 0.5507552374030098 2314 72-73 0.6298524353147528 2314 74-75 0.6437883255159917 2314 76-77 0.6866643757768287 2314 78-79 0.6722248650355347 2314 80-81 0.7134768942356366 2314 82-83 0.714437858605006 2314 84-85 0.7417481819023131 2314 86-87 0.7993899514337777 2314 88-89 0.6194399018763477 2314 90-91 0.6195234303475345 2314 92-93 0.7694976004488154 2314 94-95 0.8117232491468442 2314 96-97 0.8322280135896847 2314 98-99 0.8006216283443948 2314 100 0.8274166192566774 2315 1 0.17621379434380202 2315 2 0.15765134716008333 2315 3 0.20387993370775348 2315 4 0.13740952698272224 2315 5 0.13646971177787037 2315 6 0.13768932007826606 2315 7 0.11031419872347925 2315 8 0.1320038326740942 2315 9 -0.042353986474076066 2315 10-11 0.15302723118437456 2315 12-13 0.148673113010382 2315 14-15 0.2711630214860321 2315 16-17 0.12567422348224255 2315 18-19 0.28351636738528185 2315 20-21 0.09943357466811165 2315 22-23 0.14398578479018198 2315 24-25 0.3538862898356214 2315 26-27 0.3860047068826873 2315 28-29 0.2741282226747259 2315 30-31 0.32413684857784375 2315 32-33 0.3202604238226954 2315 34-35 0.2913371130883604 2315 36-37 0.41591477877614835 2315 38-39 0.4649686448350465 2315 40-41 0.40818327709994406 2315 42-43 0.3905570932729958 2315 44-45 0.4497342854792947 2315 46-47 0.46085842090877804 2315 48-49 0.4378474763972946 2315 50-51 0.3388422597588274 2315 52-53 0.06544307746815292 2315 54-55 0.24230223584407184 2315 56-57 0.2471604829313705 2315 58-59 0.2907695439442861 2315 60-61 0.3993416206864282 2315 62-63 0.3663084458145107 2315 64-65 0.3945557322925026 2315 66-67 0.25543441113962473 2315 68-69 0.30651541541432437 2315 70-71 0.31942486884187105 2315 72-73 0.3699495869207752 2315 74-75 0.034245202647703366 2315 76-77 0.27304496291612423 2315 78-79 0.32501925941345533 2315 80-81 0.32712470130852367 2315 82-83 0.29728738650679887 2315 84-85 0.32405469506044327 2315 86-87 0.33159618799189516 2315 88-89 0.24225535170614165 2315 90-91 0.3541020323923014 2315 92-93 0.535689271373986 2315 94-95 0.5003724255474395 2315 96-97 0.5013461280768539 2315 98-99 0.5694440892993207 2315 100 0.6542750436492604 2316 1 0.07937937711749044 2316 2 0.10511439702927561 2316 3 0.13188436536349712 2316 4 0.03247060763919052 2316 5 -0.0015271427411036598 2316 6 -0.11284997146366038 2316 7 -0.011669527710701288 2316 8 -0.029083524380141057 2316 9 0.10126684820779985 2316 10-11 0.04639633045751168 2316 12-13 0.05135648562008299 2316 14-15 -0.0778852218656425 2316 16-17 0.09865534638188223 2316 18-19 0.1491731424749574 2316 20-21 0.16933021712839746 2316 22-23 0.07933885913546845 2316 24-25 0.11184281021888154 2316 26-27 0.0343510958165254 2316 28-29 0.09013235343638826 2316 30-31 -0.4757804898327649 2316 32-33 -0.27964509374884017 2316 34-35 -0.47414125372259974 2316 36-37 -0.12449247336130043 2316 38-39 -0.09713420868600764 2316 40-41 0.12165056308587197 2316 42-43 0.13993981790686405 2316 44-45 0.03596602112402536 2316 46-47 -0.09362100282426411 2316 48-49 0.02880942298816791 2316 50-51 0.1365972063901566 2316 52-53 -0.2018555510616693 2316 54-55 -0.11861667952124577 2316 56-57 -0.11232537167526147 2316 58-59 -0.5235235783080583 2316 60-61 -0.004880252037906985 2316 62-63 0.017030085914846893 2316 64-65 -0.2996263288572649 2316 66-67 -0.6820878819935814 2316 68-69 -0.6837741551078338 2316 70-71 -0.4830533142298421 2316 72-73 -0.6329620058456271 2316 74-75 -0.3427455867904925 2316 76-77 -0.036746294972864746 2316 78-79 0.026499013540000504 2316 80-81 -0.02679277954213788 2316 82-83 0.14597078524456109 2316 84-85 0.13891928429366374 2316 86-87 0.23485128043464698 2316 88-89 0.2162584188029939 2316 90-91 -0.2987928510597051 2316 92-93 -0.39095241349803445 2316 94-95 7.810135871970658E-4 2316 96-97 0.3154044560160081 2316 98-99 0.37561073236645015 2316 100 0.2993212060275532 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 280.0 1 646.0 2 375.0 3 359.0 4 367.0 5 333.0 6 418.0 7 765.0 8 1986.0 9 3865.0 10 6584.0 11 11541.0 12 17613.0 13 23385.0 14 27463.0 15 31203.0 16 36573.0 17 43383.0 18 51803.0 19 61576.0 20 74356.0 21 90001.0 22 99976.0 23 102917.0 24 101940.0 25 105753.0 26 115882.0 27 130979.0 28 153721.0 29 182490.0 30 219971.0 31 270902.0 32 338962.0 33 439603.0 34 600254.0 35 836161.0 36 1239811.0 37 1810317.0 38 1734485.0 39 424469.0 40 423.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.60901142810353 19.41660631952499 13.721667589309778 23.252714663061706 2 15.47260830694733 20.008063135007852 40.977777143090385 23.54155141495444 3 18.996599099382063 25.629302897356087 29.413307910868337 25.96079009239351 4 12.615782096911781 16.757287846569053 38.51221629154027 32.114713764978895 5 13.421539889927248 37.62178754829924 34.77891219839927 14.177760363374246 6 30.797291337556242 37.22182858251685 17.941485981426855 14.039394098500049 7 27.52239994973124 32.324024738192435 22.171226887820797 17.982348424255527 8 25.709474044718224 35.538247798305186 20.088930388127448 18.66334776884915 9 26.17438751289825 16.25494499409529 20.77901693251168 36.79165056049478 10-11 24.939870948367364 26.386130982854617 27.9602605951646 20.71373747361342 12-13 25.4747208327889 23.994983663842223 28.220996091909296 22.30929941145958 14-15 22.933553290038407 25.499271159146065 25.97930835244484 25.587867198370684 16-17 22.060364065043334 28.379954751444437 26.8401194630022 22.719561720510033 18-19 21.754710276227655 27.580967914416295 29.016720020352533 21.64760178900352 20-21 22.809293598382112 26.737626653213365 28.918649383136536 21.53443036526799 22-23 22.758681322239184 26.533084282393176 28.61834185942474 22.089892535942898 24-25 22.340873827547707 26.723028457566535 28.95603251964624 21.980065195239526 26-27 22.259624458751347 26.947611092577752 28.620891186173345 22.171873262497556 28-29 21.98645165200104 26.931585890964598 28.92504879814433 22.15691365889003 30-31 22.438423373815425 26.799990009988413 28.811401095397315 21.950185520798847 32-33 22.32889613928601 27.081910584008945 28.474859050970093 22.114334225734957 34-35 22.518840280894363 26.98826440229008 28.663404518259433 21.829490798556122 36-37 22.471075447309886 26.80529224068274 28.494810393323867 22.228821918683515 38-39 22.37043207714334 26.980158767771755 28.67264826343024 21.976760891654664 40-41 22.406498353326793 26.63434865355488 28.852448697133497 22.10670429598483 42-43 22.24956845538689 26.675307233722474 28.6066001868105 22.468524124080133 44-45 22.28673038282527 27.068323522544645 28.447214747200345 22.197731347429738 46-47 22.295320792608948 26.96279532076598 28.133759536812352 22.608124349812726 48-49 22.50335153840566 26.936031365244084 28.13090034262064 22.42971675372962 50-51 22.474375589302202 27.043582201149725 27.90410653598548 22.577935673562596 52-53 22.317119780344328 27.330081305662763 27.801284094308095 22.551514819684815 54-55 22.276411126407268 27.211783575761533 27.841963236289097 22.6698420615421 56-57 22.46749579704477 27.144918450313106 27.662171656104956 22.725414096537175 58-59 22.240007012613383 27.13432440462417 27.998167816290405 22.62750076647204 60-61 22.367449900311843 27.36812087827821 27.736226845934937 22.528202375475008 62-63 22.33255275956022 27.197262980400765 27.788958883301795 22.681225376737217 64-65 22.178047785398512 27.0434625298035 27.84703266028413 22.931457024513865 66-67 22.32276058896024 26.99742452646211 27.672483822211056 23.007331062366593 68-69 22.131164216603917 27.075931689737516 27.764957582224614 23.027946511433953 70-71 22.27205327772422 26.826231848722927 27.776187160544797 23.125527713008058 72-73 22.416400933387145 27.042258381902425 27.644963508874408 22.896377175836022 74-75 22.433088516128265 27.185376448256832 27.549859698205758 22.831675337409145 76-77 22.348983677913786 27.14188739375696 27.48602317822833 23.023105750100928 78-79 22.30474508408539 27.038868491624857 27.491377253909217 23.16500917038054 80-81 22.254167185279666 27.32708377372165 27.457276274878577 22.961472766120103 82-83 22.60880632830365 27.055228870595478 27.714223026479058 22.621741774621814 84-85 22.621330769458954 27.13802374431286 27.34379905490773 22.89684643132045 86-87 22.25664020088904 27.345521043432637 27.555614799568694 22.84222395610963 88-89 22.5495404080064 26.944494035508715 27.524384600930645 22.98158095555424 90-91 22.642672175624778 26.979645112793488 27.492003357274346 22.885679354307385 92-93 22.68370406219948 27.05183329340205 27.411896653644703 22.852565990753767 94-95 22.359912633628213 26.8952871292698 27.626227297878973 23.118572939223014 96-97 22.549744009306426 26.888019252034184 27.471844219595916 23.090392519063478 98-99 22.429255905540124 26.98673559049275 27.35507924792841 23.228929256038715 100 22.81298044168019 26.791304021576657 27.000843744057768 23.39487179268539 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2447.0 1 1724.0 2 1617.5 3 2135.5 4 1661.0 5 1419.5 6 2133.5 7 3873.5 8 6509.0 9 8019.5 10 7229.0 11 6207.0 12 6353.0 13 7086.5 14 7894.0 15 8831.0 16 9598.5 17 10340.5 18 11272.5 19 12263.0 20 13372.0 21 14808.0 22 17179.5 23 20567.5 24 24614.0 25 29689.5 26 36773.0 27 45426.5 28 55210.5 29 68801.0 30 87741.0 31 107406.0 32 132155.0 33 162579.5 34 192464.0 35 219174.0 36 249612.0 37 283697.5 38 301074.0 39 305645.5 40 303858.0 41 301283.0 42 303961.5 43 313097.0 44 326510.0 45 345589.5 46 361531.0 47 369256.5 48 378046.5 49 380389.5 50 372302.0 51 361936.0 52 348999.5 53 334183.0 54 318845.0 55 293315.0 56 263795.5 57 229510.5 58 197260.0 59 173074.5 60 144115.5 61 115114.0 62 92056.0 63 72668.0 64 54787.0 65 38966.0 66 28248.0 67 21087.5 68 16032.5 69 12246.0 70 9402.0 71 7163.5 72 5473.5 73 4117.5 74 3063.0 75 2228.0 76 1549.5 77 1008.5 78 674.0 79 458.5 80 320.5 81 229.0 82 168.0 83 125.5 84 102.0 85 75.5 86 57.0 87 53.5 88 42.5 89 31.0 90 24.0 91 19.0 92 16.5 93 12.5 94 10.5 95 7.0 96 4.0 97 5.0 98 4.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02548464741607072 2 0.11117863726543133 3 0.01780944658608451 4 0.01787331788286664 5 0.020449460186412638 6 0.0459021719540923 7 0.046423787544479705 8 0.038588908472538166 9 0.03279791089762485 10-11 0.036869706067485776 12-13 0.035895668791558255 14-15 0.03142467801680901 16-17 0.037641484236936536 18-19 0.03632147743677247 20-21 0.046562175354174326 22-23 0.046620724042891276 24-25 0.06481872101773375 26-27 0.05764384534587425 28-29 0.050053806244930873 30-31 0.04803653778822854 32-33 0.04322490009730792 34-35 0.04437990604745148 36-37 0.03969068834202994 38-39 0.044731198179753205 40-41 0.054423667466441755 42-43 0.0557010934020844 44-45 0.05947482252029537 46-47 0.06100241103500137 48-49 0.06030514937846309 50-51 0.054700443085830996 52-53 0.06501033490808016 54-55 0.051336554788638696 56-57 0.05598319162953881 58-59 0.05423205357609536 60-61 0.04908509157706854 62-63 0.056254644640862876 64-65 0.054317215305138195 66-67 0.05256607725169475 68-69 0.058463526987911615 70-71 0.05856997914921516 72-73 0.06086934583337192 74-75 0.05157607215157169 76-77 0.051783653866113624 78-79 0.061587897922170905 80-81 0.06248209607712076 82-83 0.05281091722269291 84-85 0.05850078524436786 86-87 0.05562657688917191 88-89 0.06386065156600178 90-91 0.0693429378731348 92-93 0.07379263821562333 94-95 0.07678394394825318 96-97 0.07271747138645744 98-99 0.08179251813758537 100 0.12666742673509837 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9393891.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.54748443709911 #Duplication Level Percentage of deduplicated Percentage of total 1 72.90696987295324 41.22705744246878 2 15.238292753959382 17.233742447049565 3 5.489406360625886 9.312363622392155 4 2.480850710923432 5.611434678668361 5 1.2412480410916136 3.5094727143103883 6 0.7171787418241466 2.433279224515155 7 0.4399318521719989 1.741392769485621 8 0.3016546303562193 1.3646248412357755 9 0.22184148795285955 1.129012027876551 >10 0.8827330727471334 8.50171687646795 >50 0.0463667686277886 1.7868386768531443 >100 0.030515792141004312 3.349648429649821 >500 0.002006640249630484 0.783444818268525 >1k 8.948122815744553E-4 0.7903777027785195 >5k 2.7115523512993986E-5 0.10110676761578699 >10k+ 8.134657053898196E-5 1.124486960363811 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32350 0.34437274181699573 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26247 0.27940498777343703 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14740 0.15691048576143796 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.0645216130355355E-5 3.193564839106606E-5 2 0.0 0.0 0.0 1.0645216130355355E-5 3.193564839106606E-5 3 0.0 0.0 0.0 2.129043226071071E-5 3.193564839106606E-5 4 0.0 0.0 0.0 2.129043226071071E-5 3.193564839106606E-5 5 1.0645216130355355E-5 0.0 0.0 2.129043226071071E-5 4.258086452142142E-5 6 1.0645216130355355E-5 0.0 0.0 2.129043226071071E-5 4.258086452142142E-5 7 1.0645216130355355E-5 0.0 0.0 2.129043226071071E-5 4.258086452142142E-5 8 1.0645216130355355E-5 0.0 0.0 2.129043226071071E-5 4.258086452142142E-5 9 1.0645216130355355E-5 0.0 0.0 2.129043226071071E-5 4.258086452142142E-5 10-11 1.0645216130355355E-5 5.3226080651776775E-6 0.0 2.129043226071071E-5 5.3226080651776776E-5 12-13 1.0645216130355355E-5 1.0645216130355355E-5 0.0 2.129043226071071E-5 5.3226080651776776E-5 14-15 1.596782419553303E-5 1.0645216130355355E-5 0.0 2.129043226071071E-5 6.91939048473098E-5 16-17 2.6613040325888385E-5 1.0645216130355355E-5 0.0 2.129043226071071E-5 8.516172904284284E-5 18-19 3.193564839106606E-5 1.596782419553303E-5 0.0 3.193564839106606E-5 8.516172904284284E-5 20-21 3.193564839106606E-5 2.129043226071071E-5 0.0 9.580694517319818E-5 8.516172904284284E-5 22-23 3.193564839106606E-5 2.129043226071071E-5 0.0 3.033886597151276E-4 9.048433710802052E-5 24-25 3.193564839106606E-5 2.129043226071071E-5 0.0 7.398425210596972E-4 9.58069451731982E-5 26-27 3.725825645624374E-5 2.129043226071071E-5 0.0 0.0016500085002050802 9.58069451731982E-5 28-29 4.258086452142142E-5 2.129043226071071E-5 0.0 0.004439055126358183 1.0645216130355355E-4 30-31 5.854868871695445E-5 3.193564839106606E-5 0.0 0.014253944398545822 1.170973774339089E-4 32-33 7.451651291248748E-5 3.193564839106606E-5 0.0 0.0377692268305008 1.383878096946196E-4 34-35 7.451651291248748E-5 3.193564839106606E-5 0.0 0.07578329363199977 1.383878096946196E-4 36-37 7.451651291248748E-5 3.193564839106606E-5 0.0 0.13830264796557679 1.383878096946196E-4 38-39 7.451651291248748E-5 3.193564839106606E-5 0.0 0.22522083766992823 1.383878096946196E-4 40-41 9.58069451731982E-5 3.193564839106606E-5 0.0 0.3153432374295167 1.4903302582497497E-4 42-43 9.58069451731982E-5 3.193564839106606E-5 0.0 0.4065514492344014 1.4903302582497497E-4 44-45 9.58069451731982E-5 3.193564839106606E-5 0.0 0.521514460834174 1.4903302582497497E-4 46-47 9.58069451731982E-5 3.193564839106606E-5 0.0 0.6592954932093633 1.4903302582497497E-4 48-49 9.58069451731982E-5 4.790347258659909E-5 0.0 0.8037404308821552 1.4903302582497497E-4 50-51 9.58069451731982E-5 6.91939048473098E-5 0.0 0.9508945760601224 1.4903302582497497E-4 52-53 1.0112955323837587E-4 7.451651291248748E-5 0.0 1.094849833790918 1.4903302582497497E-4 54-55 1.0645216130355355E-4 7.451651291248748E-5 0.0 1.25667840940458 1.4903302582497497E-4 56-57 1.0645216130355355E-4 1.0112955323837587E-4 0.0 1.44974537175277 1.5967824195533032E-4 58-59 1.1709737743390891E-4 1.1177476936873123E-4 0.0 1.663394859489002 1.65000850020508E-4 60-61 1.1709737743390891E-4 1.3306520162944193E-4 0.0 1.8814461440951358 1.8096867421604104E-4 62-63 1.1709737743390891E-4 1.4903302582497497E-4 0.0 2.1020735710048157 1.8096867421604104E-4 64-65 1.1709737743390891E-4 1.4903302582497497E-4 0.0 2.330913782159065 1.8096867421604104E-4 66-67 1.1709737743390891E-4 1.4903302582497497E-4 0.0 2.587383651779651 1.916138903463964E-4 68-69 1.1709737743390891E-4 1.4903302582497497E-4 0.0 2.870535755630973 2.129043226071071E-4 70-71 1.1709737743390891E-4 1.4903302582497497E-4 0.0 3.1730036041508254 2.2887214680264014E-4 72-73 1.1709737743390891E-4 1.4903302582497497E-4 0.0 3.478196628000048 2.820982274544169E-4 74-75 1.1709737743390891E-4 1.4903302582497497E-4 0.0 3.7790623714922815 2.874208355195946E-4 76-77 1.1709737743390891E-4 1.4903302582497497E-4 0.0 4.109276975855905 2.874208355195946E-4 78-79 1.1709737743390891E-4 1.4903302582497497E-4 0.0 4.47101206518151 2.874208355195946E-4 80-81 1.1709737743390891E-4 1.5967824195533032E-4 0.0 4.851200636669086 2.9806605164994993E-4 82-83 1.1709737743390891E-4 1.7032345808568568E-4 0.0 5.239016505514062 3.087112677803053E-4 84-85 1.1709737743390891E-4 1.7564606615086336E-4 0.0 5.616942968573938 3.1403387584548297E-4 86-87 1.1709737743390891E-4 1.916138903463964E-4 0.0 6.011321613163278 3.1935648391066064E-4 88 1.1709737743390891E-4 2.0225910647675175E-4 0.0 6.326888400131533 3.1935648391066064E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 16005 0.0 51.488117 1 GTATCAA 28645 0.0 41.674595 1 CAACGCA 34590 0.0 33.62194 5 ATCAACG 34780 0.0 33.480225 3 TATCAAC 36525 0.0 32.257954 2 TCAACGC 36485 0.0 31.914965 4 AACGCAG 37095 0.0 31.54232 6 ACGCAGA 41580 0.0 27.937798 7 CGCAGAG 42570 0.0 27.143421 8 GTGGTAT 7590 0.0 26.15166 1 GTACATG 37365 0.0 24.974989 1 GTACTAG 3530 0.0 24.517248 1 GCAGAGT 46680 0.0 24.151484 9 TGGTATC 7905 0.0 24.097527 2 TACATGG 38185 0.0 24.04398 2 ACATGGG 38525 0.0 23.248606 3 GAGTACT 26445 0.0 21.67124 12-13 CAGAGTA 45975 0.0 20.709805 10-11 CATGGGG 25220 0.0 20.55123 4 GTACTTT 28140 0.0 20.307272 14-15 >>END_MODULE