FastQCFastQC Report
Mon 6 Jun 2016
ERR1211301_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211301_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9393891
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289800.3084983634576982No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249180.26525749553619477No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141950.15110884297039426No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA158300.051.199151
GTATCAA284200.041.6789361
ATCAACG345800.033.4088253
CAACGCA347350.033.240395
TATCAAC366850.031.9758342
TCAACGC366200.031.6508964
AACGCAG372500.031.4114066
ACGCAGA419600.027.4941467
CGCAGAG430250.026.8135838
GTACTAG36900.025.1819951
GTGGTAT73750.024.435461
GTACATG363500.024.3494191
GCAGAGT474550.024.0418979
TACATGG370050.023.3940982
ACATGGG375000.022.3860363
GAGTACT273800.021.70534712-13
TGGTATC80250.021.224232
GTACTTT287900.020.41422814-15
CAGAGTA468200.020.36776710-11
CATGGGG241150.020.0427514
Produced by FastQC (version 0.11.5)