##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211301_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9393891 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11621882774667 33.0 31.0 34.0 30.0 34.0 2 32.253655168023556 34.0 31.0 34.0 30.0 34.0 3 32.31642596236213 34.0 31.0 34.0 30.0 34.0 4 35.95201732700539 37.0 35.0 37.0 35.0 37.0 5 35.858312173304974 37.0 35.0 37.0 35.0 37.0 6 35.87232883583597 37.0 35.0 37.0 35.0 37.0 7 35.79205879650935 37.0 35.0 37.0 33.0 37.0 8 35.7755375275272 37.0 35.0 37.0 33.0 37.0 9 37.39317456419283 39.0 37.0 39.0 34.0 39.0 10-11 37.347404499370924 39.0 37.0 39.0 34.0 39.0 12-13 37.27917627530488 39.0 37.0 39.0 34.0 39.0 14-15 38.518848845489046 40.0 38.0 41.0 34.0 41.0 16-17 38.501476970512 40.0 38.0 41.0 34.0 41.0 18-19 38.513110754638305 40.0 38.0 41.0 34.0 41.0 20-21 38.48651362891053 40.0 38.0 41.0 34.0 41.0 22-23 38.3783332699943 40.0 38.0 41.0 34.0 41.0 24-25 38.28746064862792 40.0 38.0 41.0 34.0 41.0 26-27 38.19152782377398 40.0 38.0 41.0 33.5 41.0 28-29 38.093792550924846 40.0 37.5 41.0 33.0 41.0 30-31 38.1017930163337 40.0 37.5 41.0 33.0 41.0 32-33 38.03984387300215 40.0 37.0 41.0 33.0 41.0 34-35 37.91348792529102 40.0 37.0 41.0 33.0 41.0 36-37 37.76926430166158 40.0 37.0 41.0 33.0 41.0 38-39 37.657279395726434 40.0 37.0 41.0 33.0 41.0 40-41 37.551148187689215 40.0 37.0 41.0 32.0 41.0 42-43 37.44409233617891 40.0 36.5 41.0 32.0 41.0 44-45 37.28600715081748 40.0 36.0 41.0 31.5 41.0 46-47 37.04550499894027 39.5 36.0 41.0 31.0 41.0 48-49 35.50593263217553 39.0 35.0 41.0 24.0 41.0 50-51 36.234859229258674 39.0 35.0 40.0 29.0 41.0 52-53 36.32810562736996 39.0 35.0 40.0 29.5 41.0 54-55 36.16473791318209 38.5 35.0 40.0 29.0 41.0 56-57 35.95305747107349 38.0 35.0 40.0 28.5 41.0 58-59 35.719295763597856 38.0 34.5 40.0 28.0 41.0 60-61 35.469036153389474 38.0 34.0 40.0 28.0 41.0 62-63 35.156629718186 37.0 34.0 40.0 27.5 41.0 64-65 34.828158693772366 37.0 34.0 39.5 27.0 41.0 66-67 34.49469410492415 36.0 33.0 39.0 26.0 40.5 68-69 34.136666265342015 36.0 33.0 39.0 26.0 40.0 70-71 33.78427650480509 35.0 33.0 38.0 26.0 40.0 72-73 33.405871166697594 35.0 32.0 37.5 26.0 40.0 74-75 33.04565844973079 35.0 32.0 37.0 25.0 39.0 76-77 32.10830442891023 34.0 30.5 36.0 24.5 38.5 78-79 32.53907417064984 35.0 32.0 36.0 25.0 38.5 80-81 32.40677313586032 35.0 32.0 36.0 25.0 37.5 82-83 32.133170589269135 34.5 31.5 35.0 25.0 37.0 84-85 31.85017129749536 34.0 31.0 35.0 24.5 36.5 86-87 31.58734777740129 34.0 31.0 35.0 24.0 36.0 88-89 31.35254177422327 34.0 31.0 35.0 23.5 36.0 90-91 31.130442539731405 34.0 31.0 35.0 23.0 35.0 92-93 30.943815560559514 34.0 30.0 35.0 22.5 35.0 94-95 30.74757552541327 34.0 30.0 35.0 20.0 35.0 96-97 30.559842987320167 34.0 30.0 35.0 20.0 35.0 98-99 30.3448500200822 34.0 29.0 35.0 19.5 35.0 100 27.89391669543536 31.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.22503509526980636 1101 2 -0.20345460749773991 1101 3 -0.21308138306079627 1101 4 -0.09152482936453765 1101 5 -0.11452866256242089 1101 6 -0.22681667142187223 1101 7 -0.21710070468824938 1101 8 -0.21225040407657758 1101 9 -0.3096674272087938 1101 10-11 -0.2811863080352168 1101 12-13 -0.34503055135710525 1101 14-15 -0.4277905815969163 1101 16-17 -0.3944335263068126 1101 18-19 -0.4333423497707969 1101 20-21 -0.4479456588253896 1101 22-23 -0.5025034218147155 1101 24-25 -0.5166367159202849 1101 26-27 -0.529154124001785 1101 28-29 -0.5330440957163702 1101 30-31 -0.47127668555345537 1101 32-33 -0.43084891803326286 1101 34-35 -0.44348603664638375 1101 36-37 -0.4486515386306209 1101 38-39 -0.40951117225002776 1101 40-41 -0.4769747022910025 1101 42-43 -0.4886553693943938 1101 44-45 -0.49536915732858944 1101 46-47 -0.5381100634262239 1101 48-49 0.700390088152254 1101 50-51 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1105 68-69 -0.741357508119286 1105 70-71 -0.6990029003403038 1105 72-73 -0.7141607551569038 1105 74-75 -0.772648997867357 1105 76-77 -0.8085090223055431 1105 78-79 -0.6408013809292612 1105 80-81 -0.5982021375976849 1105 82-83 -0.647448024527435 1105 84-85 -0.6971207303451834 1105 86-87 -0.5695346041674618 1105 88-89 -0.60548839437525 1105 90-91 -0.6724488346720925 1105 92-93 -0.5943610434019284 1105 94-95 -0.6106945729505107 1105 96-97 -0.6161322780079992 1105 98-99 -0.696440036552552 1105 100 -0.747797281860052 1106 1 -0.35336631584790723 1106 2 -0.3067908309080849 1106 3 -0.3836337340691891 1106 4 -0.22611544033945563 1106 5 -0.2820940907322438 1106 6 -0.35632108642133176 1106 7 -0.2786463841777689 1106 8 -0.3506453865585897 1106 9 -0.46814736907911225 1106 10-11 -0.4576563741533022 1106 12-13 -0.4971053549959308 1106 14-15 -0.6352175130129254 1106 16-17 -0.6371710730873232 1106 18-19 -0.6618594719641706 1106 20-21 -0.6491609839530099 1106 22-23 -0.6037664453167295 1106 24-25 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0.41442842630809906 2311 4 0.27346676931647096 2311 5 0.3447978963313929 2311 6 0.3637402540003265 2311 7 0.38605014543328053 2311 8 0.17181965734909 2311 9 0.4562950788771616 2311 10-11 0.3682138272600497 2311 12-13 0.4540454199561381 2311 14-15 0.4434969412499541 2311 16-17 0.4804990861256684 2311 18-19 0.6095408131486835 2311 20-21 0.6661249554593667 2311 22-23 0.6977194447899038 2311 24-25 0.6155809004944075 2311 26-27 0.7273141439204451 2311 28-29 0.7522330861485571 2311 30-31 0.6142641708806522 2311 32-33 0.6221129438334287 2311 34-35 0.7634533601390388 2311 36-37 0.7885757068865047 2311 38-39 0.7863762544798831 2311 40-41 0.7727611480920729 2311 42-43 0.7808925778980864 2311 44-45 0.8457775718533895 2311 46-47 0.844057620541733 2311 48-49 -5.974102367900798 2311 50-51 -1.028816331860746 2311 52-53 0.3616388771319805 2311 54-55 0.7813715476795906 2311 56-57 0.8939861438103165 2311 58-59 0.9178263008022824 2311 60-61 0.7540706609083259 2311 62-63 0.6559421930329492 2311 64-65 0.7039613042056274 2311 66-67 0.8744230065475307 2311 68-69 0.8934269287629988 2311 70-71 0.8932457894106989 2311 72-73 0.3716210110447449 2311 74-75 0.7162163989672763 2311 76-77 0.8119919765388985 2311 78-79 0.6773479848395212 2311 80-81 0.7790130615550908 2311 82-83 0.797205533805375 2311 84-85 0.7923115527185836 2311 86-87 0.8389326761635694 2311 88-89 0.9423826630325038 2311 90-91 0.9769249599560723 2311 92-93 0.9494361893713084 2311 94-95 0.9865108639457567 2311 96-97 1.014423499580861 2311 98-99 1.0569817430537505 2311 100 1.2722036106734684 2312 1 0.034020977954206444 2312 2 0.18628448257188523 2312 3 0.1856100448515079 2312 4 -0.00716653123497224 2312 5 0.1785795243003463 2312 6 0.13496686255814438 2312 7 0.28185941682340143 2312 8 0.3346460416795338 2312 9 0.25826977736960544 2312 10-11 0.24470540611770275 2312 12-13 0.312625789836126 2312 14-15 0.3751790498222718 2312 16-17 0.46046854783408975 2312 18-19 0.26170909437101386 2312 20-21 0.38820140884232757 2312 22-23 0.6180208193904733 2312 24-25 0.7258089983469205 2312 26-27 0.6416875295738507 2312 28-29 0.7501950832175552 2312 30-31 0.7471780273373057 2312 32-33 0.7604417510192576 2312 34-35 0.3898012772941186 2312 36-37 0.6968461434194282 2312 38-39 0.7867576499479512 2312 40-41 0.5771929140582728 2312 42-43 0.620525975647638 2312 44-45 0.6902575524463188 2312 46-47 0.7522089274520098 2312 48-49 -4.668644413694423 2312 50-51 -0.7903034296503577 2312 52-53 0.4719099659396093 2312 54-55 0.8007208405541846 2312 56-57 0.8668337443761231 2312 58-59 0.9287411824051546 2312 60-61 0.9153200334295519 2312 62-63 0.6679314773238403 2312 64-65 0.8629959918376855 2312 66-67 0.858309153117645 2312 68-69 0.7863703138744711 2312 70-71 0.7907982718054001 2312 72-73 0.9094851572473743 2312 74-75 0.5497629965965345 2312 76-77 0.7021532390864209 2312 78-79 0.4010529068016595 2312 80-81 0.6290520458945252 2312 82-83 0.5799598004843745 2312 84-85 0.671050838957111 2312 86-87 0.7917092518970072 2312 88-89 0.8340707864924752 2312 90-91 0.6155174372482115 2312 92-93 0.8234359877838493 2312 94-95 0.9393262804620122 2312 96-97 1.0420374402135586 2312 98-99 0.9925374918666101 2312 100 0.6874968542904512 2313 1 0.04995037828613391 2313 2 0.19406511692117334 2313 3 0.29564649419017286 2313 4 0.09044411538575048 2313 5 0.1072403060745657 2313 6 0.23311825918586493 2313 7 0.15325759504784742 2313 8 0.2509619462241801 2313 9 0.4226980155593836 2313 10-11 0.22586189481744157 2313 12-13 -0.022323899785156698 2313 14-15 0.10839372644164058 2313 16-17 0.03253054174518866 2313 18-19 0.4323301725591975 2313 20-21 0.42135207045353695 2313 22-23 0.43741139379256566 2313 24-25 0.24501151732842885 2313 26-27 0.41742016309439123 2313 28-29 0.47280387387906586 2313 30-31 0.5527938141999655 2313 32-33 0.46404982638559034 2313 34-35 0.34683063635858247 2313 36-37 -0.3245313897382047 2313 38-39 0.17757040820254844 2313 40-41 0.4035291395144185 2313 42-43 0.48569646150998835 2313 44-45 0.4945609636370065 2313 46-47 0.417065481356822 2313 48-49 -5.403937537940777 2313 50-51 -1.4224212598816024 2313 52-53 0.004844259543411056 2313 54-55 0.18125617512723835 2313 56-57 0.161245544717751 2313 58-59 0.2854018544652632 2313 60-61 0.6094316614134954 2313 62-63 0.6937815604764879 2313 64-65 0.7733006853686604 2313 66-67 0.836140499639562 2313 68-69 0.8761729542895438 2313 70-71 0.810365522189656 2313 72-73 0.5086455156362177 2313 74-75 0.6501990081668438 2313 76-77 0.6999248412898211 2313 78-79 0.6906523607699526 2313 80-81 -0.20264878005549747 2313 82-83 0.19485126909044226 2313 84-85 0.5988728405119197 2313 86-87 0.6473066718473639 2313 88-89 0.6309544411419381 2313 90-91 0.7257963796994247 2313 92-93 0.760101479104133 2313 94-95 0.7088171106634498 2313 96-97 0.695222645900877 2313 98-99 0.9414320258939064 2313 100 0.9038382265623177 2314 1 0.013880189760826056 2314 2 0.1140072331441786 2314 3 0.2183864953435446 2314 4 0.04321691842484654 2314 5 0.16786726010536768 2314 6 0.22977747404551252 2314 7 0.049347909474221296 2314 8 0.19925034465129698 2314 9 0.38441319554103615 2314 10-11 0.28109438220488414 2314 12-13 0.19536590997731906 2314 14-15 0.2011154547061409 2314 16-17 0.4416404104380547 2314 18-19 0.5193978364805076 2314 20-21 0.5545399367134962 2314 22-23 0.28941032735969685 2314 24-25 0.39023485876282393 2314 26-27 0.4539843241670596 2314 28-29 0.5675838377726876 2314 30-31 0.47852623069818634 2314 32-33 0.4527418853692282 2314 34-35 0.28411610761696693 2314 36-37 0.3690945552617322 2314 38-39 0.35864542768812413 2314 40-41 0.1780119671976763 2314 42-43 0.18570156113032965 2314 44-45 0.3884800442226677 2314 46-47 0.5124214754767635 2314 48-49 -6.391172080556963 2314 50-51 -1.6655567863875405 2314 52-53 -0.9580018325634754 2314 54-55 -0.6039085680494907 2314 56-57 -0.26412306716742506 2314 58-59 0.27431152727732666 2314 60-61 0.5421757597790204 2314 62-63 0.4187628222493416 2314 64-65 0.5461821288378061 2314 66-67 0.634702910493175 2314 68-69 0.6443827651817102 2314 70-71 0.42677195919502253 2314 72-73 0.5959038314579885 2314 74-75 0.6152153624007255 2314 76-77 0.5483047209337713 2314 78-79 0.32446728682320725 2314 80-81 0.3475034962427017 2314 82-83 0.3711578933304409 2314 84-85 0.4107942339930979 2314 86-87 0.5696013320143898 2314 88-89 0.4478962373583393 2314 90-91 0.6333381193078722 2314 92-93 0.6102007582193352 2314 94-95 0.7190124519792711 2314 96-97 0.7670534049324047 2314 98-99 0.8311271044633415 2314 100 -0.5900513539624654 2315 1 0.13531094195951 2315 2 0.19120463617967687 2315 3 0.2948504404640673 2315 4 0.10738662541714916 2315 5 0.20365872692335785 2315 6 0.2783485021819132 2315 7 0.30473456873522764 2315 8 0.2237321345996861 2315 9 0.3547399685611907 2315 10-11 0.17296143273487985 2315 12-13 0.34830475065890454 2315 14-15 0.18338706777752378 2315 16-17 0.33718404524676515 2315 18-19 0.3756326552354565 2315 20-21 0.2789540379708271 2315 22-23 0.33522149326342543 2315 24-25 0.4290151394068502 2315 26-27 0.4478400357437451 2315 28-29 0.5560267536989798 2315 30-31 0.5440730799775579 2315 32-33 0.5931859765340946 2315 34-35 0.6033764816646183 2315 36-37 0.5290860524683438 2315 38-39 0.6447797156289283 2315 40-41 0.6526394912449547 2315 42-43 0.6640448259571343 2315 44-45 0.6910011124270596 2315 46-47 0.5562041381238387 2315 48-49 -4.930592278353949 2315 50-51 -0.9181730636341356 2315 52-53 0.22916300932244127 2315 54-55 0.20034462235864936 2315 56-57 0.5641204604281498 2315 58-59 0.7086390761522949 2315 60-61 0.5756350712618143 2315 62-63 0.3454494179651775 2315 64-65 0.5087356361020952 2315 66-67 -0.2094251739540809 2315 68-69 0.47879258661050983 2315 70-71 0.4703231072017502 2315 72-73 0.5837361986522254 2315 74-75 0.7152154961976223 2315 76-77 0.6365528954631401 2315 78-79 0.589161230999629 2315 80-81 0.4612455799605897 2315 82-83 0.454157963657444 2315 84-85 0.517351047546196 2315 86-87 0.5132571510132671 2315 88-89 0.49485244554733043 2315 90-91 0.5390378006120002 2315 92-93 0.5967894497215518 2315 94-95 0.2287802658456961 2315 96-97 0.4122651732399838 2315 98-99 0.3804533408742792 2315 100 0.3707211074231225 2316 1 0.2280110997392626 2316 2 0.2440970964650262 2316 3 0.2016106118641332 2316 4 -0.01567399841972872 2316 5 0.1599105811726247 2316 6 0.14545576375917335 2316 7 0.2587670130403268 2316 8 0.26383984119676995 2316 9 0.4347831894258789 2316 10-11 0.38658072501850427 2316 12-13 0.30550201918116926 2316 14-15 0.43687333031899556 2316 16-17 0.429177607630443 2316 18-19 0.0856137840349831 2316 20-21 0.45335680113936405 2316 22-23 0.5821652479833332 2316 24-25 0.5940157631088709 2316 26-27 0.5997521670852777 2316 28-29 0.6807572343540969 2316 30-31 0.6250355449153986 2316 32-33 0.5822571367210045 2316 34-35 0.5566291509407151 2316 36-37 0.486470136604062 2316 38-39 0.5552346261866887 2316 40-41 0.1990634240402187 2316 42-43 0.10772446984013584 2316 44-45 0.3233015446357044 2316 46-47 0.4467440322967775 2316 48-49 -5.052214215471299 2316 50-51 -0.9772177122707504 2316 52-53 0.2599975099899794 2316 54-55 0.5480249986701011 2316 56-57 0.4696147695685866 2316 58-59 0.3356752946974808 2316 60-61 0.44305742878804466 2316 62-63 0.4023316958935439 2316 64-65 0.41518836033140616 2316 66-67 0.49500058190534446 2316 68-69 0.15110748617762226 2316 70-71 -0.21168547038678298 2316 72-73 -0.052965610252215356 2316 74-75 0.14598339138871808 2316 76-77 0.21533067548849516 2316 78-79 0.25714782814880266 2316 80-81 0.18012499813089278 2316 82-83 0.06292569702313955 2316 84-85 0.1804289622574693 2316 86-87 0.3003695286328103 2316 88-89 0.15442414346652455 2316 90-91 0.15179781692939542 2316 92-93 0.19904699820174088 2316 94-95 0.47900981347630633 2316 96-97 0.5318149657326323 2316 98-99 0.5223519783382038 2316 100 -0.16807956415224723 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 10.0 11 21.0 12 50.0 13 189.0 14 688.0 15 2098.0 16 4990.0 17 10131.0 18 17577.0 19 26867.0 20 39059.0 21 56158.0 22 70156.0 23 74095.0 24 76424.0 25 83599.0 26 98914.0 27 121374.0 28 150110.0 29 184772.0 30 227529.0 31 281648.0 32 355734.0 33 461110.0 34 636645.0 35 902340.0 36 1406040.0 37 2107787.0 38 1759213.0 39 238533.0 40 29.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.75415663187513 20.1873118493225 14.531826328332736 23.526705190469634 2 15.242295307196093 20.467454703529153 40.56133015631821 23.72891983295654 3 18.664311232419408 25.64890119950436 29.998775798711502 25.68801176936474 4 12.640114702509 17.169004768765756 38.41567265917571 31.775207869549533 5 13.262459810026229 37.833489091714846 34.87653535733003 14.027515740928905 6 30.870291591786405 37.50882222756376 17.67886048436185 13.94202569628799 7 27.453456971171043 32.40614535749202 22.048378690484707 18.09201898085223 8 25.762163558183758 35.86343689762132 19.8489613390722 18.525438205122718 9 25.983384503090516 16.196617494158897 20.70905974054069 37.11093826220989 10-11 24.95619575567092 26.53720974881096 27.843318476164256 20.66327601935387 12-13 25.38753008806209 24.03426122966077 28.27914568027164 22.299063002005507 14-15 22.768235571269337 25.589881453657213 25.949322958066627 25.692560017006823 16-17 22.027951761056755 28.480675547465168 26.82546716007393 22.66590553140415 18-19 21.708770729902824 27.651656462779655 29.01726787764916 21.622304929668363 20-21 22.726329173321705 26.753529211133543 28.93910548608009 21.58103612946466 22-23 22.65627087466128 26.574546259713124 28.670951732859688 22.098231132765907 24-25 22.26201997107268 26.77437997710639 28.981208737128732 21.982391314692205 26-27 22.240625445103337 26.96353777339857 28.67444846573612 22.121388315761976 28-29 21.92640293095508 26.96157081512717 28.98150992814621 22.130516325771534 30-31 22.40138182833569 26.799457909906398 28.841094265732835 21.958065996025077 32-33 22.322804360466183 27.057241059592705 28.53127703313531 22.0886775468058 34-35 22.46462278276021 26.991958991361685 28.743657946900967 21.799760278977136 36-37 22.432102461160508 26.79220440229792 28.59992884286715 22.175764293674423 38-39 22.28575727095041 26.95586817149065 28.781164125069512 21.97721043248943 40-41 22.333849296795737 26.612834715327626 28.987824468804256 22.06549151907237 42-43 22.211241679382177 26.670737262602835 28.673673797821298 22.44434726019369 44-45 22.234765238700398 27.037730933718894 28.52112139996885 22.206382427611853 46-47 22.23798316353872 26.917423024167686 28.22829305030854 22.616300761985055 48-49 22.58614731510733 26.960215542353357 28.127937735560906 22.32569940697841 50-51 22.422026655355136 27.013663701648095 27.967084120157782 22.59722552283899 52-53 22.24267375515911 27.2435446976637 27.883373345581898 22.630408201595294 54-55 22.254647170815886 27.181495145360262 27.86446314707814 22.699394536745714 56-57 22.435716828971387 27.08698236609934 27.702556284021007 22.774744520908268 58-59 22.139312910459648 27.118367811020995 28.04575855881023 22.696560719709133 60-61 22.30372790978871 27.374525385552044 27.76054584627059 22.561200858388656 62-63 22.299989087821743 27.145422075070464 27.785312208065964 22.769276629041833 64-65 22.04532616119228 27.020762117534865 27.875365008278518 23.058546712994346 66-67 22.188156000743675 26.994157074516465 27.72024215045895 23.097444774280905 68-69 22.024556839488746 27.0576989413918 27.801952721133294 23.115791497986162 70-71 22.10388973306883 26.800731113482897 27.824973591610693 23.270405561837585 72-73 22.197857483581352 26.992576471289727 27.714017393970884 23.095548651158037 74-75 22.2093438520057 27.14804800867624 27.571719418390938 23.07088872092712 76-77 22.080409891319583 27.088039085493236 27.550603066466007 23.280947956721178 78-79 22.02184424649614 26.978819748929457 27.59499302522682 23.404342979347582 80-81 21.93981257925589 27.25482199415263 27.525335269966096 23.280030156625386 82-83 22.23626288832803 26.963824606469046 27.799235958977526 23.0006765462254 84-85 22.266659994079856 27.015745885178976 27.450663673940497 23.26693044680067 86-87 21.868498401324448 27.241913734182077 27.670287548644783 23.21930031584869 88-89 22.027604927299507 26.828174334231708 27.666375584633236 23.47784515383555 90-91 22.077630588179176 26.843852108525073 27.68109410083169 23.39742320246406 92-93 22.089897539429153 26.899510387790436 27.584461765931028 23.42613030684938 94-95 21.66884011796307 26.73353611308341 27.85660512461118 23.741018644342336 96-97 21.828785235046865 26.71986385539469 27.740346918145836 23.71100399141261 98-99 21.64215808269561 26.812455632617983 27.668344520057715 23.877041764628693 100 21.665986025468936 26.673176684693455 27.61162027568897 24.049217014148642 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2229.0 1 1525.0 2 1248.5 3 1662.5 4 1297.0 5 1111.5 6 1789.0 7 3498.0 8 5816.5 9 7164.0 10 6531.0 11 5457.5 12 5578.0 13 6332.5 14 7155.0 15 8091.0 16 8985.0 17 9971.0 18 11074.5 19 12288.5 20 13665.0 21 15163.5 22 17358.5 23 20644.0 24 24758.0 25 30158.0 26 37160.0 27 45672.5 28 55885.5 29 69531.0 30 88634.0 31 108517.0 32 132916.5 33 163297.0 34 193676.0 35 220802.0 36 251385.0 37 285074.0 38 302803.0 39 307340.5 40 304246.5 41 300797.5 42 304228.5 43 313504.5 44 328440.5 45 349974.5 46 365972.5 47 373966.0 48 382989.0 49 384996.0 50 376506.5 51 364022.5 52 349469.0 53 334579.5 54 319619.5 55 294091.5 56 263334.0 57 228187.5 58 196067.5 59 171506.0 60 141450.5 61 111666.5 62 88013.5 63 68590.0 64 50912.0 65 35603.0 66 25339.0 67 19099.5 68 14522.0 69 10731.0 70 8100.5 71 6054.0 72 4554.0 73 3440.5 74 2564.0 75 1794.5 76 1174.5 77 669.5 78 387.0 79 233.0 80 135.0 81 73.0 82 51.5 83 37.5 84 22.0 85 15.0 86 7.0 87 6.0 88 5.0 89 2.5 90 1.0 91 1.5 92 1.5 93 0.5 94 0.0 95 0.5 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02995563819081997 2 0.0017883963098997 3 1.1709737743390891E-4 4 0.020683654941280456 5 7.238746968641641E-4 6 5.322608065177677E-5 7 3.193564839106606E-5 8 0.004279376884402853 9 0.010357795294835761 10-11 0.0011656511662739115 12-13 7.983912097766516E-5 14-15 1.2774259356426425E-4 16-17 3.033886597151276E-4 18-19 3.725825645624375E-5 20-21 7.45165129124875E-5 22-23 2.767756193892392E-4 24-25 4.2580864521421426E-5 26-27 5.322608065177677E-5 28-29 9.90005100123048E-4 30-31 0.002655981424523661 32-33 0.009628597989906418 34-35 0.018347030000667456 36-37 0.021295754868775888 38-39 0.020885914047757206 40-41 0.004907444636093819 42-43 0.005407769794220521 44-45 0.009389080626973422 46-47 0.011938609890193531 48-49 1.194270829840372 50-51 0.018171383934516592 52-53 0.018810096902337915 54-55 0.009772308407666217 56-57 0.007510199979965703 58-59 0.008122299907461135 60-61 0.005077768094179505 62-63 0.007621974749334435 64-65 0.019262518587878016 66-67 0.028795309632611236 68-69 0.029162569589108494 70-71 0.026213844721000062 72-73 0.022684955573787264 74-75 0.00937311280277789 76-77 0.014397654816305618 78-79 0.008026492962287938 80-81 0.00284227270680488 82-83 0.014184750493698512 84-85 0.0237494771868228 86-87 0.023531250256150514 88-89 0.03265420047986505 90-91 0.020470750618673348 92-93 0.013343778419400439 94-95 0.0065201948798426545 96-97 0.003720503037559197 98-99 0.015259917322864403 100 0.03444791939782993 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9393891.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.061864708426576 #Duplication Level Percentage of deduplicated Percentage of total 1 72.34307900725604 40.55687907895803 2 15.615870807486576 17.50909673027161 3 5.501636384427188 9.252959839761427 4 2.5035536286225035 5.61415539272501 5 1.287791232156256 3.6097988914920998 6 0.7445395889742417 2.5044166624284885 7 0.4789868820224168 1.879702844393627 8 0.31031236603095 1.3917351905423097 9 0.21668450117221724 1.0932963470216772 >10 0.9166471707495075 8.723820244501997 >50 0.04818946204204441 1.8563238677184524 >100 0.02934725391240251 3.2077575153533933 >500 0.002397318580151698 0.9155065442817085 >1k 8.541798173677766E-4 0.736747884666534 >5k 2.7554187533826664E-5 0.09594374759236822 >10k+ 8.266256260147999E-5 1.051859218291278 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28980 0.3084983634576982 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24918 0.26525749553619477 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14195 0.15110884297039426 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.0645216130355355E-5 0.0 2 0.0 0.0 0.0 1.0645216130355355E-5 0.0 3 0.0 1.0645216130355355E-5 0.0 1.0645216130355355E-5 0.0 4 0.0 1.0645216130355355E-5 0.0 1.0645216130355355E-5 0.0 5 0.0 2.129043226071071E-5 0.0 1.0645216130355355E-5 0.0 6 0.0 2.129043226071071E-5 0.0 1.0645216130355355E-5 0.0 7 0.0 2.129043226071071E-5 0.0 1.0645216130355355E-5 1.0645216130355355E-5 8 0.0 2.129043226071071E-5 0.0 1.0645216130355355E-5 1.0645216130355355E-5 9 0.0 2.129043226071071E-5 0.0 1.0645216130355355E-5 1.0645216130355355E-5 10-11 1.0645216130355355E-5 2.129043226071071E-5 0.0 1.596782419553303E-5 1.0645216130355355E-5 12-13 1.0645216130355355E-5 2.129043226071071E-5 0.0 3.193564839106606E-5 2.129043226071071E-5 14-15 1.0645216130355355E-5 2.129043226071071E-5 0.0 3.193564839106606E-5 3.725825645624374E-5 16-17 1.0645216130355355E-5 2.129043226071071E-5 0.0 3.193564839106606E-5 4.258086452142142E-5 18-19 1.0645216130355355E-5 3.725825645624374E-5 0.0 3.725825645624374E-5 4.79034725865991E-5 20-21 2.129043226071071E-5 4.258086452142142E-5 0.0 1.0645216130355354E-4 6.387129678213213E-5 22-23 2.129043226071071E-5 4.258086452142142E-5 0.0 2.874208355195946E-4 6.387129678213213E-5 24-25 2.129043226071071E-5 4.258086452142142E-5 0.0 7.238746968641641E-4 6.387129678213213E-5 26-27 2.129043226071071E-5 4.258086452142142E-5 0.0 0.0016287180679443692 6.387129678213213E-5 28-29 2.129043226071071E-5 4.258086452142142E-5 0.0 0.0043858290457064065 6.387129678213213E-5 30-31 3.725825645624374E-5 4.258086452142142E-5 0.0 0.014174105277568155 6.387129678213213E-5 32-33 4.258086452142142E-5 4.258086452142142E-5 0.0 0.03762019380467582 6.387129678213213E-5 34-35 4.79034725865991E-5 4.258086452142142E-5 0.0 0.07544796932389358 6.387129678213213E-5 36-37 5.3226080651776776E-5 4.258086452142142E-5 0.0 0.13697731855734752 6.387129678213213E-5 38-39 5.3226080651776776E-5 5.3226080651776776E-5 0.0 0.21981306787570773 6.387129678213213E-5 40-41 6.387129678213213E-5 5.3226080651776776E-5 0.0 0.3060393185315861 6.387129678213213E-5 42-43 6.387129678213213E-5 5.3226080651776776E-5 0.0 0.3932928325440438 6.91939048473098E-5 44-45 6.387129678213213E-5 5.3226080651776776E-5 0.0 0.5033483995077226 7.451651291248748E-5 46-47 6.387129678213213E-5 5.854868871695445E-5 0.0 0.6348540769740676 7.451651291248748E-5 48-49 6.387129678213213E-5 6.387129678213213E-5 0.0 0.7743330213220485 7.451651291248748E-5 50-51 6.387129678213213E-5 6.387129678213213E-5 5.3226080651776775E-6 0.9211411969757792 7.451651291248748E-5 52-53 6.387129678213213E-5 7.451651291248748E-5 1.0645216130355355E-5 1.0646706460613604 7.983912097766516E-5 54-55 6.387129678213213E-5 7.451651291248748E-5 1.0645216130355355E-5 1.2266163190524566 8.516172904284284E-5 56-57 6.387129678213213E-5 7.451651291248748E-5 1.0645216130355355E-5 1.4195129579425607 9.58069451731982E-5 58-59 6.387129678213213E-5 7.983912097766516E-5 1.0645216130355355E-5 1.6330613161255543 1.0645216130355355E-4 60-61 6.387129678213213E-5 9.58069451731982E-5 1.0645216130355355E-5 1.8507400181671259 1.0645216130355355E-4 62-63 6.387129678213213E-5 1.0645216130355355E-4 1.0645216130355355E-5 2.070984217296113 1.1709737743390891E-4 64-65 6.387129678213213E-5 1.0645216130355355E-4 1.0645216130355355E-5 2.2993134580761048 1.1709737743390891E-4 66-67 6.387129678213213E-5 1.0645216130355355E-4 1.0645216130355355E-5 2.5550807434320877 1.2774259356426425E-4 68-69 8.516172904284284E-5 1.0645216130355355E-4 1.0645216130355355E-5 2.8377112316930226 1.437104177597973E-4 70-71 8.516172904284284E-5 1.0645216130355355E-4 1.0645216130355355E-5 3.13959891593377 1.5967824195533032E-4 72-73 8.516172904284284E-5 1.0645216130355355E-4 1.0645216130355355E-5 3.444557745028125 1.8096867421604104E-4 74-75 8.516172904284284E-5 1.0645216130355355E-4 1.0645216130355355E-5 3.745412843304228 1.8096867421604104E-4 76-77 8.516172904284284E-5 1.0645216130355355E-4 1.0645216130355355E-5 4.075270832927485 1.8096867421604104E-4 78-79 8.516172904284284E-5 1.0645216130355355E-4 1.0645216130355355E-5 4.43699527703696 1.8096867421604104E-4 80-81 8.516172904284284E-5 1.0645216130355355E-4 1.0645216130355355E-5 4.816609006853497 1.9693649841157407E-4 82-83 8.516172904284284E-5 1.0645216130355355E-4 1.0645216130355355E-5 5.204142777471018 2.129043226071071E-4 84-85 8.516172904284284E-5 1.1177476936873123E-4 1.0645216130355355E-5 5.582617469161607 2.129043226071071E-4 86-87 8.516172904284284E-5 1.2774259356426425E-4 1.0645216130355355E-5 5.977608213678443 2.1822693067228478E-4 88 8.516172904284284E-5 1.383878096946196E-4 1.0645216130355355E-5 6.2936753258048235 2.2354953873746246E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15830 0.0 51.19915 1 GTATCAA 28420 0.0 41.678936 1 ATCAACG 34580 0.0 33.408825 3 CAACGCA 34735 0.0 33.24039 5 TATCAAC 36685 0.0 31.975834 2 TCAACGC 36620 0.0 31.650896 4 AACGCAG 37250 0.0 31.411406 6 ACGCAGA 41960 0.0 27.494146 7 CGCAGAG 43025 0.0 26.813583 8 GTACTAG 3690 0.0 25.181995 1 GTGGTAT 7375 0.0 24.43546 1 GTACATG 36350 0.0 24.349419 1 GCAGAGT 47455 0.0 24.041897 9 TACATGG 37005 0.0 23.394098 2 ACATGGG 37500 0.0 22.386036 3 GAGTACT 27380 0.0 21.705347 12-13 TGGTATC 8025 0.0 21.22423 2 GTACTTT 28790 0.0 20.414228 14-15 CAGAGTA 46820 0.0 20.367767 10-11 CATGGGG 24115 0.0 20.042751 4 >>END_MODULE