FastQCFastQC Report
Mon 6 Jun 2016
ERR1211300_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211300_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9049071
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325550.35976068703627145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258300.28544366598516024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146870.16230395363236733No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA158100.049.4160961
GTATCAA281150.041.155481
CAACGCA341450.033.099745
ATCAACG341950.033.0236743
TATCAAC356900.032.0763852
TCAACGC356450.031.838694
AACGCAG363700.031.3712926
ACGCAGA408650.027.5761957
CGCAGAG420800.026.7113388
GTACATG381900.026.2953451
GTGGTAT75700.026.2830121
GTACTAG33800.026.1620521
TACATGG391550.025.357852
ACATGGG394250.024.3637083
GCAGAGT457550.023.9815799
TGGTATC81900.022.7991162
GAGTACT251400.022.15026512-13
CATGGGG272950.021.4005374
GTACTTT270100.020.55594314-15
CAGAGTA456700.020.13752410-11
Produced by FastQC (version 0.11.5)