FastQCFastQC Report
Mon 6 Jun 2016
ERR1211300_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211300_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9049071
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284590.314496372058524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238840.26393869602747067No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141720.15661276168570232No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA156700.051.095841
GTATCAA281150.041.7995641
ATCAACG349800.032.9854933
CAACGCA351300.032.9171525
TATCAAC364900.032.0686382
TCAACGC366500.031.5852434
AACGCAG375150.031.1118986
ACGCAGA423400.027.3891567
CGCAGAG438950.026.3333858
GTGGTAT68950.026.069941
GTACTAG34950.024.4399171
GCAGAGT477600.023.8865769
GTACATG374100.023.761171
TACATGG381800.023.066742
TGGTATC78300.022.5311812
ACATGGG381550.022.1240183
GAGTACT266200.021.4256812-13
CATGGGG262150.020.745444
CAGAGTA472300.020.17033210-11
GTACTTT282100.020.07673614-15
Produced by FastQC (version 0.11.5)