##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211300_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9049071 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09772660641076 33.0 31.0 34.0 30.0 34.0 2 32.238661405132085 34.0 31.0 34.0 30.0 34.0 3 32.30001311736862 34.0 31.0 34.0 30.0 34.0 4 35.941766508407326 37.0 35.0 37.0 35.0 37.0 5 35.8434433766737 37.0 35.0 37.0 35.0 37.0 6 35.85684210014487 37.0 35.0 37.0 35.0 37.0 7 35.77490009747962 37.0 35.0 37.0 33.0 37.0 8 35.75874672659768 37.0 35.0 37.0 33.0 37.0 9 37.371954093409144 39.0 37.0 39.0 34.0 39.0 10-11 37.3237860549442 39.0 37.0 39.0 34.0 39.0 12-13 37.251186945046626 39.0 37.0 39.0 33.5 39.0 14-15 38.478925239950044 40.0 38.0 41.0 34.0 41.0 16-17 38.46107340742492 40.0 38.0 41.0 34.0 41.0 18-19 38.47120820468753 40.0 38.0 41.0 34.0 41.0 20-21 38.440982063241634 40.0 38.0 41.0 34.0 41.0 22-23 38.3351077696263 40.0 38.0 41.0 34.0 41.0 24-25 38.24072631323149 40.0 38.0 41.0 33.5 41.0 26-27 38.139443374905554 40.0 38.0 41.0 33.0 41.0 28-29 38.03738240091165 40.0 37.0 41.0 33.0 41.0 30-31 38.04471116427311 40.0 37.0 41.0 33.0 41.0 32-33 37.98415804230069 40.0 37.0 41.0 33.0 41.0 34-35 37.85725258427081 40.0 37.0 41.0 33.0 41.0 36-37 37.71501599445954 40.0 37.0 41.0 33.0 41.0 38-39 37.59869781107917 40.0 37.0 41.0 32.5 41.0 40-41 37.49147332361521 40.0 36.0 41.0 32.0 41.0 42-43 37.3851010230774 40.0 36.0 41.0 32.0 41.0 44-45 37.217934249825205 39.5 36.0 41.0 31.0 41.0 46-47 36.97146093781339 39.0 36.0 41.0 31.0 41.0 48-49 35.423863178883224 39.0 35.0 40.5 24.0 41.0 50-51 36.14372414582668 39.0 35.0 40.0 29.0 41.0 52-53 36.232439329959945 39.0 35.0 40.0 29.5 41.0 54-55 36.06263620873347 38.0 35.0 40.0 29.0 41.0 56-57 35.848161319543195 38.0 35.0 40.0 28.5 41.0 58-59 35.60193350234516 38.0 34.0 40.0 28.0 41.0 60-61 35.34432031752209 37.5 34.0 40.0 28.0 41.0 62-63 35.02448577318047 37.0 34.0 40.0 27.5 41.0 64-65 34.68603103014663 36.5 33.5 39.0 26.5 41.0 66-67 34.34533749376041 36.0 33.0 39.0 26.0 40.5 68-69 33.98180907189257 35.5 33.0 39.0 26.0 40.0 70-71 33.62521854453347 35.0 32.5 38.0 26.0 40.0 72-73 33.25332793830439 35.0 32.0 37.0 25.5 39.5 74-75 32.89114385333036 35.0 32.0 37.0 25.0 39.0 76-77 31.964399881490596 34.0 30.5 36.0 24.5 38.0 78-79 32.40489034730747 34.5 31.5 36.0 25.0 38.0 80-81 32.280997739989004 35.0 32.0 35.5 25.0 37.0 82-83 32.014642829081566 34.0 31.0 35.0 25.0 37.0 84-85 31.740329587423947 34.0 31.0 35.0 24.0 36.5 86-87 31.48539678824489 34.0 31.0 35.0 24.0 36.0 88-89 31.260426015001983 34.0 31.0 35.0 23.0 36.0 90-91 31.041418063799036 34.0 30.5 35.0 23.0 35.0 92-93 30.848907362976817 34.0 30.0 35.0 21.0 35.0 94-95 30.653289547623174 34.0 30.0 35.0 20.0 35.0 96-97 30.465107965226487 34.0 29.5 35.0 20.0 35.0 98-99 30.249682425963947 34.0 29.0 35.0 19.0 35.0 100 27.784871507804503 31.0 25.0 34.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.29826171881491703 1101 2 -0.28365177510865536 1101 3 -0.31186234792402345 1101 4 -0.17531723865383952 1101 5 -0.1789659026781294 1101 6 -0.22528557526592152 1101 7 -0.22369610678003227 1101 8 -0.23875982783895466 1101 9 -0.3048115604186634 1101 10-11 -0.2780980489338063 1101 12-13 -0.3819636356392877 1101 14-15 -0.5024559454416178 1101 16-17 -0.5547221884763829 1101 18-19 -0.5270725038259201 1101 20-21 -0.5800610480341035 1101 22-23 -0.6425898909877787 1101 24-25 -0.6123550977616077 1101 26-27 -0.6787185449264186 1101 28-29 -0.6346570426867331 1101 30-31 -0.5466927624060602 1101 32-33 -0.6311659651526611 1101 34-35 -0.7153944913977028 1101 36-37 -0.7016494399605264 1101 38-39 -0.6307000882602907 1101 40-41 -0.6150439453781118 1101 42-43 -0.7350425646222263 1101 44-45 -0.6716857838008394 1101 46-47 -0.6577772019398722 1101 48-49 0.5361736472119674 1101 50-51 -0.35772224761224436 1101 52-53 -0.5872188319787952 1101 54-55 -0.6751763839764635 1101 56-57 -0.682912634505719 1101 58-59 -0.7752041321874685 1101 60-61 -0.7924001860491359 1101 62-63 -0.7550064584494223 1101 64-65 -0.7768984434072337 1101 66-67 -0.7381504709352456 1101 68-69 -0.7656805709673549 1101 70-71 -0.7685482060591227 1101 72-73 -0.893480895617131 1101 74-75 -0.8379871154808498 1101 76-77 -0.8186508316994647 1101 78-79 -0.7418623179661843 1101 80-81 -0.6482258532472969 1101 82-83 -0.7151915640570827 1101 84-85 -0.694673518975133 1101 86-87 -0.6685731766038217 1101 88-89 -0.7002211800537097 1101 90-91 -0.7917291411240903 1101 92-93 -0.8298631107171914 1101 94-95 -0.8185658516119254 1101 96-97 -0.9178172491285999 1101 98-99 -1.0899666582686756 1101 100 -0.9157538730235579 1102 1 -0.25167896414270885 1102 2 -0.2902976164619204 1102 3 -0.27918296613932014 1102 4 -0.22413236186289964 1102 5 -0.18653481710843067 1102 6 -0.2502482623231046 1102 7 -0.2520400196953503 1102 8 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1105 68-69 -0.673326646279385 1105 70-71 -0.6502234380664476 1105 72-73 -0.6358240090389842 1105 74-75 -0.7487461792816603 1105 76-77 -0.7267750594797917 1105 78-79 -0.5758646730978896 1105 80-81 -0.5319716296434578 1105 82-83 -0.5899708562937711 1105 84-85 -0.5529182881763468 1105 86-87 -0.5886759362767755 1105 88-89 -0.7340247360675889 1105 90-91 -0.7208598802316821 1105 92-93 -0.669595589484917 1105 94-95 -0.6179052428148104 1105 96-97 -0.6962875396624888 1105 98-99 -0.8084950778599413 1105 100 -0.8532418658040406 1106 1 -0.29532513615562905 1106 2 -0.29788732182646527 1106 3 -0.3522320956777385 1106 4 -0.26283767030712113 1106 5 -0.2713495980432512 1106 6 -0.36257311994281594 1106 7 -0.3693588860977499 1106 8 -0.3979440612526872 1106 9 -0.4886676761818265 1106 10-11 -0.471945942736518 1106 12-13 -0.473436807937766 1106 14-15 -0.645442313841194 1106 16-17 -0.5952282146392562 1106 18-19 -0.6941836193191833 1106 20-21 -0.6797510806218909 1106 22-23 -0.6251809200635563 1106 24-25 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1.2120971106497649 2209 78-79 1.0691289513405167 2209 80-81 1.040754566074284 2209 82-83 1.0457904750462568 2209 84-85 1.1292780523349997 2209 86-87 1.1093213449717076 2209 88-89 1.1346018535677835 2209 90-91 1.183762655829998 2209 92-93 1.1745452889419106 2209 94-95 1.1704198064737206 2209 96-97 1.2172818261686267 2209 98-99 1.2488327945328486 2209 100 1.3627390917746176 2210 1 0.5188279319943518 2210 2 0.4520172849024746 2210 3 0.4651347738234932 2210 4 0.3208113902475489 2210 5 0.36029691681946474 2210 6 0.3989241762981095 2210 7 0.4360170916180124 2210 8 0.4377002184942924 2210 9 0.5801773356761544 2210 10-11 0.5829357208201742 2210 12-13 0.602930643921944 2210 14-15 0.7646717308452793 2210 16-17 0.7461925974367034 2210 18-19 0.7618791096655144 2210 20-21 0.7524910870833068 2210 22-23 0.7673342661421003 2210 24-25 0.7303906755958636 2210 26-27 0.7692836076533709 2210 28-29 0.7836642690208535 2210 30-31 0.8293606800693567 2210 32-33 0.8042053904166053 2210 34-35 0.8650694156626741 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0.33357762668660484 2311 2 0.40590097200654895 2311 3 0.40019488745938503 2311 4 0.2805555056885183 2311 5 0.34216128626476205 2311 6 0.393332762827761 2311 7 0.37295777047503975 2311 8 0.21943501911933083 2311 9 0.49324431488954446 2311 10-11 0.3636229399762314 2311 12-13 0.49307556089858195 2311 14-15 0.44681119531687585 2311 16-17 0.49178698546231914 2311 18-19 0.632262093364929 2311 20-21 0.6868123502354635 2311 22-23 0.6933792921985287 2311 24-25 0.6385327114131201 2311 26-27 0.6976340960631546 2311 28-29 0.7797958537211471 2311 30-31 0.6570308075640696 2311 32-33 0.7057473726922439 2311 34-35 0.8080622476359736 2311 36-37 0.7904418960128012 2311 38-39 0.8354792124273658 2311 40-41 0.8353659493688426 2311 42-43 0.8405649550324199 2311 44-45 0.8927406171685419 2311 46-47 0.964722184542687 2311 48-49 -6.042201744463004 2311 50-51 -1.0347564616891844 2311 52-53 0.4336609746099853 2311 54-55 0.8902580736983552 2311 56-57 0.9634335193381673 2311 58-59 1.0290022664824576 2311 60-61 0.8950161558995688 2311 62-63 0.7670008121022249 2311 64-65 0.8376395570590915 2311 66-67 0.9487361981095077 2311 68-69 1.0145984176917366 2311 70-71 0.9520801825050995 2311 72-73 0.501076045139925 2311 74-75 0.7921207304415887 2311 76-77 0.8589214285310902 2311 78-79 0.8424642724520979 2311 80-81 0.8735904187623689 2311 82-83 0.8820918459490734 2311 84-85 0.9431586556517964 2311 86-87 0.9407904032344838 2311 88-89 0.9490448320850753 2311 90-91 0.9893710274600842 2311 92-93 0.9898182621315748 2311 94-95 1.0523451618424566 2311 96-97 1.1122410719704554 2311 98-99 1.0622618685650131 2311 100 1.312458008544585 2312 1 0.04046450381195399 2312 2 0.22508300285748106 2312 3 0.19541582302078098 2312 4 -7.005415919820734E-4 2312 5 0.16195030992251702 2312 6 0.10241415094460393 2312 7 0.27622833579632555 2312 8 0.30579442874488905 2312 9 0.26663150375196665 2312 10-11 0.21843648009895134 2312 12-13 0.2739510339281921 2312 14-15 0.2811620751842909 2312 16-17 0.4219329356970576 2312 18-19 0.2811453114811542 2312 20-21 0.32497797930108874 2312 22-23 0.6187162889182503 2312 24-25 0.6893547656881083 2312 26-27 0.6276745638362442 2312 28-29 0.7204352867859924 2312 30-31 0.7376375786531426 2312 32-33 0.7504186670748112 2312 34-35 0.38287943515857137 2312 36-37 0.7071179500000255 2312 38-39 0.7928225854833855 2312 40-41 0.5921658189264818 2312 42-43 0.6332971468142787 2312 44-45 0.7500086632812639 2312 46-47 0.8921591101224138 2312 48-49 -4.596032660977606 2312 50-51 -0.7393998691162196 2312 52-53 0.5733902134538695 2312 54-55 0.8641286215486659 2312 56-57 0.9870178324165835 2312 58-59 1.0120563543869778 2312 60-61 1.0394534238571822 2312 62-63 0.8808876233783494 2312 64-65 1.0194616507294256 2312 66-67 0.9891623158524894 2312 68-69 0.9207687467932075 2312 70-71 0.9762421892925062 2312 72-73 1.0064642196472136 2312 74-75 0.6562096950253746 2312 76-77 0.8327214786983035 2312 78-79 0.5092900287475501 2312 80-81 0.6829669579403657 2312 82-83 0.7283185617780923 2312 84-85 0.7457969827922604 2312 86-87 0.843452201055289 2312 88-89 0.8784851251103127 2312 90-91 0.7025293674354351 2312 92-93 0.91216437668486 2312 94-95 1.0773811029572649 2312 96-97 1.1106248494244433 2312 98-99 1.1276788511556965 2312 100 0.5721183750012209 2313 1 0.09575913104018952 2313 2 0.24090168967204306 2313 3 0.2784580121394882 2313 4 0.06208613722057521 2313 5 0.13702130981535277 2313 6 0.23307940654422055 2313 7 0.12215767527405319 2313 8 0.22602046739741155 2313 9 0.41622068676200286 2313 10-11 0.19683465107026876 2313 12-13 -0.010629486201843008 2313 14-15 0.11761225915885376 2313 16-17 0.15163143174964233 2313 18-19 0.4164740097271391 2313 20-21 0.32278301091613315 2313 22-23 0.4568119735536982 2313 24-25 0.23525307062148215 2313 26-27 0.40056242865959035 2313 28-29 0.4002597071768008 2313 30-31 0.5283249826469927 2313 32-33 0.4423592861581582 2313 34-35 0.3772041324043087 2313 36-37 -0.3278309658003522 2313 38-39 0.14073501001809063 2313 40-41 0.35415140460861494 2313 42-43 0.455294833736005 2313 44-45 0.42631925878728083 2313 46-47 0.3606338180851125 2313 48-49 -5.153549718813494 2313 50-51 -1.27071283128533 2313 52-53 0.0675894329015918 2313 54-55 0.2989174526850249 2313 56-57 0.30844484095443647 2313 58-59 0.34627316751883797 2313 60-61 0.6849873519820733 2313 62-63 0.7207554028232082 2313 64-65 0.8052270652973448 2313 66-67 0.8462125159025007 2313 68-69 0.8992287635831246 2313 70-71 0.8757262120839187 2313 72-73 0.6251458989956262 2313 74-75 0.8201848405974772 2313 76-77 0.7786432269925143 2313 78-79 0.6518462035055208 2313 80-81 -0.2792713694986375 2313 82-83 0.18443125066459132 2313 84-85 0.5594003836424726 2313 86-87 0.7511985572872071 2313 88-89 0.747867426042685 2313 90-91 0.7779178574150727 2313 92-93 0.8422484602057416 2313 94-95 0.7329918806516851 2313 96-97 0.7719323638030353 2313 98-99 1.0016245697281967 2313 100 1.065893157982682 2314 1 0.062073337322431144 2314 2 0.17866440266273287 2314 3 0.20500828922442338 2314 4 0.03901530052606006 2314 5 0.1970784653445321 2314 6 0.2729922708266912 2314 7 0.07114905056110388 2314 8 0.1855698141692841 2314 9 0.38069300453963706 2314 10-11 0.25793782927930664 2314 12-13 0.1498719247214595 2314 14-15 0.16738159328959767 2314 16-17 0.4344785721960065 2314 18-19 0.46991862132916395 2314 20-21 0.54062259450059 2314 22-23 0.2662879731766168 2314 24-25 0.3727512548111065 2314 26-27 0.41639677807405207 2314 28-29 0.554313542477388 2314 30-31 0.4570438975187159 2314 32-33 0.4366772484634751 2314 34-35 0.31040008229722105 2314 36-37 0.392210384451694 2314 38-39 0.445579534152138 2314 40-41 0.2584157966443925 2314 42-43 0.23640893771472093 2314 44-45 0.46619146050759497 2314 46-47 0.542174289794012 2314 48-49 -6.471577026283509 2314 50-51 -1.6345762151213705 2314 52-53 -0.9061205304113145 2314 54-55 -0.45179921289988556 2314 56-57 -0.049517255742486554 2314 58-59 0.3775391041524614 2314 60-61 0.5782752668754227 2314 62-63 0.49643492156392455 2314 64-65 0.6379531645966807 2314 66-67 0.7089059576590273 2314 68-69 0.7139727381834078 2314 70-71 0.520676945671319 2314 72-73 0.6810529619571071 2314 74-75 0.6359456690088052 2314 76-77 0.5805750174279041 2314 78-79 0.44498976147392 2314 80-81 0.5221110417121011 2314 82-83 0.5010888769136379 2314 84-85 0.585014513892478 2314 86-87 0.6535324917696776 2314 88-89 0.5050973454979726 2314 90-91 0.749341831366312 2314 92-93 0.8023776106846547 2314 94-95 0.7548082628156401 2314 96-97 0.7936380588025251 2314 98-99 0.9037810763562923 2314 100 -0.4462293079959885 2315 1 0.15546266718992996 2315 2 0.2466189540920496 2315 3 0.3076186458465102 2315 4 0.11037589304443429 2315 5 0.21960000561514903 2315 6 0.2783185314897523 2315 7 0.3388047883957128 2315 8 0.22886777191752827 2315 9 0.42481409477473164 2315 10-11 0.2172402562818192 2315 12-13 0.3887370784602311 2315 14-15 0.2400083841972318 2315 16-17 0.3670245037690236 2315 18-19 0.4290231423902142 2315 20-21 0.3536676191398769 2315 22-23 0.46368103475688827 2315 24-25 0.49489808778908184 2315 26-27 0.5621974444440809 2315 28-29 0.6359679172539714 2315 30-31 0.5690471833065018 2315 32-33 0.6583490799958369 2315 34-35 0.6916451490913076 2315 36-37 0.5446935548572114 2315 38-39 0.6788801544190335 2315 40-41 0.6715356275365352 2315 42-43 0.7342650805301858 2315 44-45 0.7242612250207259 2315 46-47 0.603887527247501 2315 48-49 -4.866094287711494 2315 50-51 -0.9218426882173745 2315 52-53 0.24377311157010695 2315 54-55 0.2028164916683295 2315 56-57 0.5143155299670923 2315 58-59 0.6416353216188568 2315 60-61 0.562938453554743 2315 62-63 0.3120343592134489 2315 64-65 0.4967930975843302 2315 66-67 -0.25628468990372966 2315 68-69 0.5345996745549471 2315 70-71 0.5957298899737111 2315 72-73 0.6583833751016854 2315 74-75 0.777083298404456 2315 76-77 0.7313108448400811 2315 78-79 0.7238970805329572 2315 80-81 0.5189587649931369 2315 82-83 0.5331250767639695 2315 84-85 0.564685833800052 2315 86-87 0.5519917098743754 2315 88-89 0.5735116369874973 2315 90-91 0.6260938786923553 2315 92-93 0.7111430260454874 2315 94-95 0.35591288861577297 2315 96-97 0.45297368278453476 2315 98-99 0.3855642145979026 2315 100 0.3535329148889126 2316 1 0.19295652884684245 2316 2 0.22349590947782616 2316 3 0.1681695044380973 2316 4 -0.048888556400690675 2316 5 0.12784237067113935 2316 6 0.12320613975136041 2316 7 0.2248794717649787 2316 8 0.2901186161288294 2316 9 0.45289885632809757 2316 10-11 0.39116408350895426 2316 12-13 0.31290548679157126 2316 14-15 0.47686932194929454 2316 16-17 0.43724891498456486 2316 18-19 0.08809301988658547 2316 20-21 0.4306208136635803 2316 22-23 0.5875674686472507 2316 24-25 0.6066090417832015 2316 26-27 0.6343444298582952 2316 28-29 0.684025552331569 2316 30-31 0.5781479350850347 2316 32-33 0.5458362311776597 2316 34-35 0.5414236935511099 2316 36-37 0.4754318988955859 2316 38-39 0.5752022160519914 2316 40-41 0.1931674798723364 2316 42-43 0.127957966459185 2316 44-45 0.39506123173075736 2316 46-47 0.4304514994226949 2316 48-49 -5.3078856480714265 2316 50-51 -1.06502826734053 2316 52-53 0.2609377169462448 2316 54-55 0.5341572246860338 2316 56-57 0.3746135880302859 2316 58-59 0.3409370861290668 2316 60-61 0.4758418843894674 2316 62-63 0.43000353392059054 2316 64-65 0.4777105129971275 2316 66-67 0.5357785910642932 2316 68-69 0.24155740121580038 2316 70-71 -0.10208636659396575 2316 72-73 0.0230363248322476 2316 74-75 0.2013685090795363 2316 76-77 0.258719690439591 2316 78-79 0.2868115075092632 2316 80-81 0.25376608398266143 2316 82-83 0.1630281874378916 2316 84-85 0.3208741053528321 2316 86-87 0.36079903157157744 2316 88-89 0.16735315036859078 2316 90-91 0.20926327108624676 2316 92-93 0.30483546457959676 2316 94-95 0.5589391752533253 2316 96-97 0.6638748681631306 2316 98-99 0.6788559576597848 2316 100 -0.1450722217047371 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 9.0 11 29.0 12 37.0 13 180.0 14 687.0 15 2088.0 16 4797.0 17 9867.0 18 16994.0 19 26113.0 20 38120.0 21 54518.0 22 68493.0 23 73307.0 24 76066.0 25 83505.0 26 98731.0 27 121280.0 28 147871.0 29 183681.0 30 224844.0 31 278395.0 32 351062.0 33 456911.0 34 631706.0 35 892545.0 36 1382489.0 37 2025515.0 38 1597414.0 39 201805.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.80772049598123 20.166719911212418 14.520683018822039 23.50487657398431 2 15.411511155177893 20.364205916039225 40.1137067389024 24.110576189880483 3 18.606308279534005 25.502063197724407 30.046800441150793 25.844828081590794 4 12.874939041940472 16.93665799953223 38.00111061936926 32.18729233915804 5 13.551995468243055 37.761178575071426 34.420179225859485 14.266646730826038 6 31.253251716088332 37.0749893801218 17.487944614848733 14.183814288941138 7 27.747547538034294 32.46149051781011 21.668602408866324 18.122359535289263 8 26.307881981903726 35.38969183908343 19.601149953463 18.70127622554984 9 26.353523572655252 16.03334668034597 20.46223029354844 37.15089945345033 10-11 25.228271609426876 26.314349939368853 27.494715149631798 20.962663301572476 12-13 25.641356316923353 23.774898290596873 28.03492957559704 22.54881581688274 14-15 23.082712458531603 25.24855067819199 25.68650224443202 25.98223461884439 16-17 22.365449162162736 28.263443342209964 26.487277521351015 22.883829974276285 18-19 22.091072457781433 27.365161688723905 28.722820617081123 21.820945236413532 20-21 23.048998385302525 26.4220380374168 28.645135488329814 21.883828088950864 22-23 23.05328097569622 26.194341370349978 28.375897304533538 22.37648034942026 24-25 22.669365435509935 26.39380568013412 28.61101829918441 22.32581058517154 26-27 22.53733023179794 26.62022651728772 28.399625530434548 22.442817720479784 28-29 22.311616636780222 26.70326344714531 28.619961300587153 22.365158615487314 30-31 22.78362863659699 26.454709247124626 28.53838574057079 22.223276375707595 32-33 22.746408907464495 26.68792249142964 28.185681185948148 22.379987415157725 34-35 22.80236901615047 26.69159799718913 28.409912279068056 22.09612070759234 36-37 22.796097922551013 26.522959575189013 28.31272615253269 22.36821634972728 38-39 22.709306950588893 26.593026702238188 28.46967802093604 22.22798832623688 40-41 22.634383974104296 26.279017594424776 28.672977768396347 22.413620663074585 42-43 22.534259439715548 26.343156420004522 28.376495520366795 22.746088619913134 44-45 22.520982278193383 26.763546377186877 28.21769249701958 22.497778847600156 46-47 22.56327274401175 26.549091033076234 27.93443163601557 22.95320458689644 48-49 22.926533217463025 26.62301154566454 27.814287538257975 22.63616769861446 50-51 22.756907264158976 26.71099314737853 27.64984710823707 22.882252480225425 52-53 22.546997181321395 26.921566701846494 27.5816237164921 22.94981240034001 54-55 22.512891517183863 26.835006263125543 27.60796127582531 23.044140943865287 56-57 22.76421313126647 26.734220728224944 27.381288406139543 23.120277734369044 58-59 22.49480732679172 26.738205497900935 27.754327153995114 23.01266002131223 60-61 22.642307014131973 26.983333824201356 27.496844400123265 22.877514761543406 62-63 22.638532347754666 26.837107204888934 27.433226544136858 23.091133903219546 64-65 22.32019581897897 26.68089958640309 27.557654958191836 23.441249636426107 66-67 22.525390204103786 26.604669245173096 27.434934018051166 23.435006532671952 68-69 22.283630361445677 26.684472288074122 27.549397030463187 23.482500320017014 70-71 22.39235440364932 26.424067983062155 27.573312726242115 23.610264887046405 72-73 22.489140710253782 26.704659097472057 27.344541312278054 23.461658879996108 74-75 22.483082363993038 26.83262509325008 27.262205398834027 23.422087143922855 76-77 22.332056099128124 26.740036575719934 27.193892485863007 23.734014839288932 78-79 22.23863035488702 26.618772896763538 27.331996184722346 23.810600563627094 80-81 22.19510575184007 26.92298426337295 27.206424347202606 23.675485637584366 82-83 22.476140708685012 26.585046694575155 27.492246583431935 23.446566013307898 84-85 22.48294601135711 26.686001401248742 27.145400310083073 23.68565227731107 86-87 22.06544046620687 26.87970924191091 27.37596926087195 23.678881031010267 88-89 22.181561618842032 26.454635573170155 27.387600757559216 23.976202050428594 90-91 22.301398074173193 26.414512904556105 27.391988775350164 23.89210024592055 92-93 22.305137699325215 26.514417375431343 27.24145884869222 23.938986076551227 94-95 21.885999911256114 26.340749673661755 27.46228232812461 24.310968086957516 96-97 21.939801441554803 26.27486504659776 27.448488449509416 24.33684506233802 98-99 21.765647236392844 26.349231647315086 27.368367185887017 24.51675393040505 100 21.76035673683694 26.2233147129404 27.327427515997133 24.68890103422553 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2065.0 1 1468.0 2 1169.0 3 1447.0 4 1196.0 5 1110.5 6 1861.0 7 3620.5 8 6001.5 9 7329.0 10 6553.5 11 5556.0 12 5653.5 13 6340.5 14 7265.5 15 8025.5 16 8841.5 17 9827.0 18 10758.5 19 11654.5 20 12686.5 21 14058.0 22 16120.0 23 18743.0 24 21615.0 25 25693.5 26 31650.0 27 39265.0 28 47992.0 29 60402.5 30 78003.0 31 95658.0 32 117369.5 33 143263.0 34 167683.5 35 191078.0 36 218752.0 37 250756.5 38 267178.0 39 269539.0 40 268729.0 41 267245.5 42 272833.0 43 286667.5 44 303499.0 45 327201.0 46 350025.5 47 364104.0 48 376954.5 49 384765.5 50 380811.5 51 373416.0 52 364763.5 53 352136.0 54 336231.0 55 309125.5 56 277511.5 57 241891.5 58 207195.0 59 179666.0 60 148724.5 61 118245.5 62 94295.0 63 73297.5 64 53471.0 65 37365.0 66 26796.0 67 20099.5 68 15181.5 69 11162.0 70 8540.0 71 6537.0 72 4947.5 73 3813.5 74 2803.5 75 1865.5 76 1188.0 77 711.5 78 415.5 79 232.5 80 133.5 81 82.0 82 56.0 83 42.0 84 28.5 85 15.5 86 11.5 87 7.5 88 4.0 89 2.5 90 2.0 91 2.0 92 1.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03051141934901384 2 0.001978103608646678 3 1.2155943963750533E-4 4 0.020631952163929317 5 4.641360422522931E-4 6 3.315257444659236E-5 7 1.105085814886412E-5 8 0.003945156359144492 9 0.009437432859129959 10-11 0.0010166789496954991 12-13 6.630514889318472E-5 14-15 1.2155943963750533E-4 16-17 3.149494572426274E-4 18-19 1.657628722329618E-5 20-21 8.288143611648091E-5 22-23 2.76271453721603E-4 24-25 7.183057796761678E-5 26-27 4.972886166988855E-5 28-29 0.0010664078113653875 30-31 0.0023151547821870333 32-33 0.009774484032670314 34-35 0.01863727226805934 36-37 0.021455241096019692 38-39 0.021499444528615148 40-41 0.00514969989737068 42-43 0.005597259652399678 44-45 0.009282720845045863 46-47 0.011929401371698818 48-49 1.2002944832679507 50-51 0.01831679738174228 52-53 0.019300323756991186 54-55 0.009647399163958377 56-57 0.007879261860140119 58-59 0.00823288932090377 60-61 0.005243632191636025 62-63 0.007901363576437847 64-65 0.019178764317353682 66-67 0.028947722920949562 68-69 0.029583147264509252 70-71 0.02593636407538409 72-73 0.023134971534647036 74-75 0.009072754540217443 76-77 0.013984860987387545 78-79 0.007873736431065686 80-81 0.00270746024647171 82-83 0.014205878150364828 84-85 0.02365436186764365 86-87 0.023206802112614652 88-89 0.03219667521671562 90-91 0.020333578993909984 92-93 0.012918453176022157 94-95 0.0061940059924383405 96-97 0.0033097320155848044 98-99 0.01550435398285636 100 0.033981388807757175 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9049071.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.575082479919764 #Duplication Level Percentage of deduplicated Percentage of total 1 71.7203691389434 39.14145061248128 2 15.495624874920397 16.913500112533544 3 5.63810495801323 9.2310012934205 4 2.63304709343514 5.747950491909431 5 1.390674856635621 3.794809750181981 6 0.8197621353402672 2.684315169006614 7 0.5195804373035344 1.9849301654555223 8 0.3498826174905313 1.527589816626877 9 0.2600434743514786 1.277270465498716 >10 1.0837699679719082 10.099077752172457 >50 0.05625940641438262 2.1103738936407153 >100 0.03023073748214597 3.0917218232091317 >500 0.0018930877457088836 0.7066579821385848 >1k 6.4071949845506E-4 0.5403404508691211 >5k 2.9123613493994506E-5 0.09180510762973562 >10k+ 8.737084048198352E-5 1.0572051132257385 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28459 0.314496372058524 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23884 0.26393869602747067 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14172 0.15661276168570232 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.105085814886412E-5 1.105085814886412E-5 2 0.0 0.0 0.0 1.105085814886412E-5 1.105085814886412E-5 3 0.0 0.0 0.0 1.105085814886412E-5 1.105085814886412E-5 4 0.0 0.0 0.0 2.210171629772824E-5 1.105085814886412E-5 5 0.0 0.0 0.0 2.210171629772824E-5 1.105085814886412E-5 6 0.0 0.0 0.0 2.210171629772824E-5 2.210171629772824E-5 7 0.0 0.0 0.0 2.210171629772824E-5 2.210171629772824E-5 8 0.0 0.0 0.0 2.210171629772824E-5 2.210171629772824E-5 9 0.0 0.0 0.0 3.315257444659236E-5 6.630514889318472E-5 10-11 0.0 5.52542907443206E-6 0.0 4.420343259545648E-5 6.630514889318472E-5 12-13 0.0 1.105085814886412E-5 0.0 4.420343259545648E-5 6.630514889318472E-5 14-15 5.52542907443206E-6 2.7627145372160302E-5 0.0 4.420343259545648E-5 1.2155943963750533E-4 16-17 1.105085814886412E-5 4.420343259545648E-5 0.0 6.630514889318472E-5 1.4366115593523356E-4 18-19 1.105085814886412E-5 4.420343259545648E-5 0.0 1.3813572686080153E-4 1.6576287223296182E-4 20-21 1.105085814886412E-5 4.420343259545648E-5 0.0 2.3206802112614653E-4 1.6576287223296182E-4 22-23 2.210171629772824E-5 4.420343259545648E-5 0.0 5.52542907443206E-4 1.82339159456258E-4 24-25 3.8678003521024426E-5 4.420343259545648E-5 0.0 0.0013205775487892626 1.989154466795542E-4 26-27 4.420343259545648E-5 4.420343259545648E-5 0.0 0.0023648836438569217 2.099663048284183E-4 28-29 4.420343259545648E-5 4.972886166988854E-5 0.0 0.005751971666483774 2.099663048284183E-4 30-31 4.972886166988854E-5 7.735600704204885E-5 0.0 0.016990694403878587 2.099663048284183E-4 32-33 6.630514889318472E-5 8.288143611648091E-5 0.0 0.04250712586960584 2.3206802112614653E-4 34-35 8.840686519091297E-5 8.840686519091297E-5 0.0 0.08240072378700532 2.7074602464717095E-4 36-37 1.1050858148864121E-4 8.840686519091297E-5 0.0 0.14559505611128482 2.76271453721603E-4 38-39 1.1050858148864121E-4 8.840686519091297E-5 0.0 0.23185252939224368 2.8179688279603507E-4 40-41 1.1050858148864121E-4 8.840686519091297E-5 0.0 0.3235525503115182 2.873223118704671E-4 42-43 1.1050858148864121E-4 8.840686519091297E-5 0.0 0.4156338258369284 2.983731700193313E-4 44-45 1.1050858148864121E-4 8.840686519091297E-5 0.0 0.5310489883436653 3.260003153914916E-4 46-47 1.1050858148864121E-4 8.840686519091297E-5 0.0 0.6674386796169463 3.591528898380839E-4 48-49 1.1050858148864121E-4 8.840686519091297E-5 0.0 0.8122546502287362 3.978308933591084E-4 50-51 1.1050858148864121E-4 8.840686519091297E-5 0.0 0.9663643925437209 3.978308933591084E-4 52-53 1.4366115593523356E-4 8.840686519091297E-5 0.0 1.118076098640402 4.4755975502899694E-4 54-55 1.547120140840977E-4 9.393229426534502E-5 0.0 1.2908286386525203 4.972886166988855E-4 56-57 1.547120140840977E-4 9.94577233397771E-5 0.0 1.492412867575025 4.972886166988855E-4 58-59 1.547120140840977E-4 1.0498315241420915E-4 0.0 1.7169552543017952 4.972886166988855E-4 60-61 1.547120140840977E-4 1.1603401056307328E-4 0.0 1.9446305593137683 4.972886166988855E-4 62-63 1.547120140840977E-4 1.2155943963750533E-4 0.0 2.1742894933634624 5.304411911454778E-4 64-65 1.547120140840977E-4 1.2155943963750533E-4 0.0 2.4106507728804427 5.414920492943419E-4 66-67 1.7681373038182593E-4 1.2155943963750533E-4 0.0 2.67822520123889 5.47017478368774E-4 68-69 2.099663048284183E-4 1.2155943963750533E-4 0.0 2.973874334724526 5.52542907443206E-4 70-71 2.099663048284183E-4 1.2155943963750533E-4 0.0 3.2856908736819506 5.580683365176381E-4 72-73 2.099663048284183E-4 1.2155943963750533E-4 0.0 3.5980102266851484 6.520006307829832E-4 74-75 2.099663048284183E-4 1.3261029778636945E-4 0.0 3.9107329360107794 6.575260598574152E-4 76-77 2.2101716297728241E-4 1.3261029778636945E-4 0.0 4.250248450918332 6.796277761551434E-4 78-79 2.3206802112614653E-4 1.3261029778636945E-4 0.0 4.620584809203066 7.017294924528717E-4 80-81 2.3206802112614653E-4 1.3261029778636945E-4 0.0 5.0122216965697355 7.514583541227602E-4 82-83 2.3206802112614653E-4 1.3261029778636945E-4 0.0 5.409339809578244 8.17763503015945E-4 84-85 2.3206802112614653E-4 1.3261029778636945E-4 0.0 5.802590122234648 8.17763503015945E-4 86-87 2.3206802112614653E-4 1.381357268608015E-4 0.0 6.209206447822102 8.17763503015945E-4 88 2.3206802112614653E-4 1.4366115593523356E-4 0.0 6.529388486398217 8.17763503015945E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15670 0.0 51.09584 1 GTATCAA 28115 0.0 41.799564 1 ATCAACG 34980 0.0 32.985493 3 CAACGCA 35130 0.0 32.917152 5 TATCAAC 36490 0.0 32.068638 2 TCAACGC 36650 0.0 31.585243 4 AACGCAG 37515 0.0 31.111898 6 ACGCAGA 42340 0.0 27.389156 7 CGCAGAG 43895 0.0 26.333385 8 GTGGTAT 6895 0.0 26.06994 1 GTACTAG 3495 0.0 24.439917 1 GCAGAGT 47760 0.0 23.886576 9 GTACATG 37410 0.0 23.76117 1 TACATGG 38180 0.0 23.06674 2 TGGTATC 7830 0.0 22.531181 2 ACATGGG 38155 0.0 22.124018 3 GAGTACT 26620 0.0 21.42568 12-13 CATGGGG 26215 0.0 20.74544 4 CAGAGTA 47230 0.0 20.170332 10-11 GTACTTT 28210 0.0 20.076736 14-15 >>END_MODULE