FastQCFastQC Report
Mon 6 Jun 2016
ERR1211299_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211299_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9347365
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350550.37502547509378314No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT275970.29523828373022776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160700.17192010796625573No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA165900.052.449321
GTATCAA303600.041.4106141
CAACGCA370000.033.070255
ATCAACG371850.033.0071683
TATCAAC387050.032.129522
TCAACGC390150.031.5434424
AACGCAG394250.031.3819356
ACGCAGA442350.027.5348997
CGCAGAG455200.026.5606148
GTACATG401550.025.8627051
GTACTAG36000.025.6075531
TACATGG417350.024.624132
GCAGAGT487650.024.2644989
ACATGGG418750.023.9128593
GTGGTAT79100.023.7252581
GAGTACT268000.022.09336712-13
TGGTATC83400.021.655842
CATGGGG286700.021.093864
CAGAGTA483600.020.71264810-11
GTACTTT292250.020.13996314-15
Produced by FastQC (version 0.11.5)