FastQCFastQC Report
Mon 6 Jun 2016
ERR1211299_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211299_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9347365
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT309130.33071352193907055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261100.2793300571872394No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155140.16597190759106978No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA164700.054.1898161
GTATCAA295050.039.903021
CAACGCA366100.031.2134065
ATCAACG366050.031.184233
TATCAAC380400.030.5729142
TCAACGC387150.029.6543254
AACGCAG392450.029.4523546
GTACTAG37350.027.1371291
ACGCAGA443450.025.716037
CGCAGAG452900.025.1276638
GTACATG388100.024.701621
GTGGTAT75050.024.447071
TACATGG400800.023.5973722
ACATGGG400700.022.972753
GCAGAGT495400.022.782119
TGGTATC77750.022.6272452
GAGTACT277000.021.77531412-13
CATGGGG271650.020.6045954
CAGAGTA489150.020.36684610-11
GTACTTT294100.020.36574414-15
Produced by FastQC (version 0.11.5)