##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211299_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9347365 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11192726506347 33.0 31.0 34.0 30.0 34.0 2 32.24723908823503 34.0 31.0 34.0 30.0 34.0 3 32.30970129014968 34.0 31.0 34.0 30.0 34.0 4 35.94891137769842 37.0 35.0 37.0 35.0 37.0 5 35.85230800337849 37.0 35.0 37.0 35.0 37.0 6 35.868621691781584 37.0 35.0 37.0 35.0 37.0 7 35.787764894170714 37.0 35.0 37.0 33.0 37.0 8 35.771269443313706 37.0 35.0 37.0 33.0 37.0 9 37.388285789631624 39.0 37.0 39.0 34.0 39.0 10-11 37.338910644871575 39.0 37.0 39.0 34.0 39.0 12-13 37.26905989014016 39.0 37.0 39.0 34.0 39.0 14-15 38.49921384261768 40.0 38.0 41.0 34.0 41.0 16-17 38.4819668965532 40.0 38.0 41.0 34.0 41.0 18-19 38.493320844965396 40.0 38.0 41.0 34.0 41.0 20-21 38.46588054494502 40.0 38.0 41.0 34.0 41.0 22-23 38.35834205682564 40.0 38.0 41.0 34.0 41.0 24-25 38.26530674687466 40.0 38.0 41.0 34.0 41.0 26-27 38.164505344554314 40.0 38.0 41.0 33.0 41.0 28-29 38.0654942328667 40.0 37.5 41.0 33.0 41.0 30-31 38.07004214556723 40.0 37.0 41.0 33.0 41.0 32-33 38.00687541355238 40.0 37.0 41.0 33.0 41.0 34-35 37.87790714281512 40.0 37.0 41.0 33.0 41.0 36-37 37.733140943998656 40.0 37.0 41.0 33.0 41.0 38-39 37.616789758397154 40.0 37.0 41.0 33.0 41.0 40-41 37.50867506511193 40.0 36.5 41.0 32.0 41.0 42-43 37.40114230053069 40.0 36.0 41.0 32.0 41.0 44-45 37.23824377244283 40.0 36.0 41.0 31.0 41.0 46-47 36.989230119932195 39.0 36.0 41.0 31.0 41.0 48-49 35.44203751538535 39.0 35.0 40.5 24.0 41.0 50-51 36.16154365428118 39.0 35.0 40.0 29.0 41.0 52-53 36.25078928660644 39.0 35.0 40.0 29.5 41.0 54-55 36.079042061586335 38.5 35.0 40.0 29.0 41.0 56-57 35.863360369473114 38.0 35.0 40.0 28.5 41.0 58-59 35.61885643708146 38.0 34.0 40.0 28.0 41.0 60-61 35.36311126183689 37.5 34.0 40.0 28.0 41.0 62-63 35.044371488649475 37.0 34.0 40.0 27.5 41.0 64-65 34.710284235182854 36.5 34.0 39.0 27.0 41.0 66-67 34.37099877880023 36.0 33.0 39.0 26.0 40.5 68-69 34.011776794850746 35.5 33.0 39.0 26.0 40.0 70-71 33.65770299972238 35.0 32.5 38.0 26.0 40.0 72-73 33.28612801575631 35.0 32.0 37.0 26.0 39.5 74-75 32.92659829802302 35.0 32.0 37.0 25.0 39.0 76-77 32.0009389277085 34.0 30.5 36.0 24.5 38.0 78-79 32.44021400683508 35.0 31.5 36.0 25.0 38.0 80-81 32.316810566400264 35.0 32.0 35.5 25.0 37.0 82-83 32.05535527926855 34.0 31.5 35.0 25.0 37.0 84-85 31.77486398573288 34.0 31.0 35.0 24.0 36.5 86-87 31.522555714899333 34.0 31.0 35.0 24.0 36.0 88-89 31.295665837377697 34.0 31.0 35.0 23.0 36.0 90-91 31.077808451900616 34.0 31.0 35.0 23.0 35.0 92-93 30.88992074236964 34.0 30.0 35.0 21.0 35.0 94-95 30.695960091426834 34.0 30.0 35.0 20.0 35.0 96-97 30.510146763285697 34.0 30.0 35.0 20.0 35.0 98-99 30.29725842523535 34.0 29.0 35.0 19.5 35.0 100 27.839482142828487 31.0 25.0 34.0 14.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.23324299825949169 1101 2 -0.25365017647511934 1101 3 -0.24524190587461447 1101 4 -0.1129611407692579 1101 5 -0.19221744199883517 1101 6 -0.2166812830876026 1101 7 -0.22515009047567958 1101 8 -0.2166114417677676 1101 9 -0.3109832856332986 1101 10-11 -0.3712684756485629 1101 12-13 -0.36032796648731136 1101 14-15 -0.4749119990088744 1101 16-17 -0.4658677127531732 1101 18-19 -0.49827949125636195 1101 20-21 -0.5352207575565657 1101 22-23 -0.5648270023120716 1101 24-25 -0.5184732910847032 1101 26-27 -0.6402626856220408 1101 28-29 -0.5751683451924023 1101 30-31 -0.5229191088073648 1101 32-33 -0.44116868344875826 1101 34-35 -0.591379253825032 1101 36-37 -0.6194574198552303 1101 38-39 -0.5698516757984393 1101 40-41 -0.5752166047267693 1101 42-43 -0.6085758632521419 1101 44-45 -0.5889243152455208 1101 46-47 -0.5906913991021696 1101 48-49 0.6283216521468873 1101 50-51 -0.3043308969750882 1101 52-53 -0.6307434223169821 1101 54-55 -0.6233518488305876 1101 56-57 -0.6550108691364827 1101 58-59 -0.7878541931087639 1101 60-61 -0.6792164465477555 1101 62-63 -0.78439318358528 1101 64-65 -0.7621992369322328 1101 66-67 -0.7074073745864169 1101 68-69 -0.8175268643000564 1101 70-71 -0.8306264469891076 1101 72-73 -0.824528743177126 1101 74-75 -0.8549191484668626 1101 76-77 -0.7659763044693833 1101 78-79 -0.722202960203127 1101 80-81 -0.599505350530535 1101 82-83 -0.6083680991660145 1101 84-85 -0.7587921907178021 1101 86-87 -0.6803843094766364 1101 88-89 -0.7394435903800449 1101 90-91 -0.7664043384387398 1101 92-93 -0.7683341003507174 1101 94-95 -0.7709773362090253 1101 96-97 -0.9723127355452021 1101 98-99 -1.007675845407359 1101 100 -0.9765007524828313 1102 1 -0.281962529478065 1102 2 -0.3055396191765496 1102 3 -0.3357645183969389 1102 4 -0.19122681794249274 1102 5 -0.17933443450592534 1102 6 -0.22644050817517325 1102 7 -0.180118566872423 1102 8 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1105 68-69 -0.7954079032183898 1105 70-71 -0.7504771232222467 1105 72-73 -0.6531642796656811 1105 74-75 -0.7734431372868897 1105 76-77 -0.7925413745952277 1105 78-79 -0.661731557037804 1105 80-81 -0.7121846152369145 1105 82-83 -0.5955077522538517 1105 84-85 -0.5507995966222872 1105 86-87 -0.5531993078008632 1105 88-89 -0.505140711283989 1105 90-91 -0.5551508648092032 1105 92-93 -0.5287961525452758 1105 94-95 -0.5263768983358084 1105 96-97 -0.5851435009952333 1105 98-99 -0.615350369732031 1105 100 -0.6957395314385906 1106 1 -0.27530827255417023 1106 2 -0.23970813835272864 1106 3 -0.2654887650408426 1106 4 -0.13165485519630948 1106 5 -0.24588634311847812 1106 6 -0.2626972524989313 1106 7 -0.3241937297235751 1106 8 -0.3415147596003436 1106 9 -0.39466843140276353 1106 10-11 -0.4122497194475656 1106 12-13 -0.4331916834528826 1106 14-15 -0.5647031618077918 1106 16-17 -0.536754315426883 1106 18-19 -0.5766698761346163 1106 20-21 -0.6252181632478582 1106 22-23 -0.6415181149915483 1106 24-25 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0.2692107594133333 2311 5 0.34576364557215555 2311 6 0.35882240686113676 2311 7 0.4174602231025091 2311 8 0.20466190396204098 2311 9 0.4506809838423038 2311 10-11 0.395171817779314 2311 12-13 0.5007609595411111 2311 14-15 0.47250755915429465 2311 16-17 0.48563565556654 2311 18-19 0.628492654235977 2311 20-21 0.6600271284626515 2311 22-23 0.6875267049132887 2311 24-25 0.6471497268401265 2311 26-27 0.7475612356128423 2311 28-29 0.7687118774832484 2311 30-31 0.6399583030997391 2311 32-33 0.6268237284283273 2311 34-35 0.7316523335276983 2311 36-37 0.807989544065471 2311 38-39 0.822318316512245 2311 40-41 0.8171396392909287 2311 42-43 0.8586950680389407 2311 44-45 0.8421999475349438 2311 46-47 0.8627929751168324 2311 48-49 -6.024997444641567 2311 50-51 -1.0441912152866735 2311 52-53 0.38443431884316226 2311 54-55 0.8315829346495818 2311 56-57 0.9549235041160529 2311 58-59 0.9513954731511021 2311 60-61 0.8442077663742751 2311 62-63 0.7208975985456689 2311 64-65 0.7997561490356873 2311 66-67 0.9542545496317061 2311 68-69 0.9703344480136664 2311 70-71 0.9528640717385244 2311 72-73 0.45423868206945883 2311 74-75 0.7404437046469425 2311 76-77 0.9155006650251813 2311 78-79 0.866891694762657 2311 80-81 0.8367637837413611 2311 82-83 0.8149147188091703 2311 84-85 0.8754314634150155 2311 86-87 0.9189356461180616 2311 88-89 0.9453491037020036 2311 90-91 0.9728379665933389 2311 92-93 0.9599714653026297 2311 94-95 1.0126222100534719 2311 96-97 1.1016721500454842 2311 98-99 1.1110212091405565 2311 100 1.2919080399039622 2312 1 0.1070076353947016 2312 2 0.24538315143060885 2312 3 0.23758893996106423 2312 4 0.02964119957539424 2312 5 0.2546716800415325 2312 6 0.1553997054637719 2312 7 0.3223701618327013 2312 8 0.3721525298668169 2312 9 0.2909171248375557 2312 10-11 0.2462143791877054 2312 12-13 0.31638158528986793 2312 14-15 0.35796823644880504 2312 16-17 0.5222812536907213 2312 18-19 0.36839120521901947 2312 20-21 0.3686788316746217 2312 22-23 0.6563341842237023 2312 24-25 0.7592747792080132 2312 26-27 0.7035060302030942 2312 28-29 0.7939320455077024 2312 30-31 0.822399476711368 2312 32-33 0.8875130288206208 2312 34-35 0.5212911965676525 2312 36-37 0.8149050804712132 2312 38-39 0.9070242841475746 2312 40-41 0.6276638051271277 2312 42-43 0.7298709088316286 2312 44-45 0.8633634103806855 2312 46-47 0.8743602892197089 2312 48-49 -4.617343112052772 2312 50-51 -0.6478199434905036 2312 52-53 0.6581424864511902 2312 54-55 0.8873383106131243 2312 56-57 1.0227390787637418 2312 58-59 1.0653640918876093 2312 60-61 1.0955945044479662 2312 62-63 0.8375878557776133 2312 64-65 1.0725300256487031 2312 66-67 1.0219819831955732 2312 68-69 0.9336805008640638 2312 70-71 0.9770033195705992 2312 72-73 1.0531609460102516 2312 74-75 0.7377594705043933 2312 76-77 0.9371423994109271 2312 78-79 0.5691605141130438 2312 80-81 0.8098305916182937 2312 82-83 0.7646699872149725 2312 84-85 0.8370738477485666 2312 86-87 0.945691163071384 2312 88-89 1.0067680352223043 2312 90-91 0.8477230214531772 2312 92-93 0.974679293629265 2312 94-95 1.1315645697120154 2312 96-97 1.2008278721493681 2312 98-99 1.2561005793440039 2312 100 0.7456723593363428 2313 1 0.09650564675725093 2313 2 0.23046319531093218 2313 3 0.32462645999383 2313 4 0.06058652087173755 2313 5 0.15991946110609945 2313 6 0.24553437360142993 2313 7 0.18947684640804852 2313 8 0.26682686150640933 2313 9 0.43439471869045576 2313 10-11 0.25588075337084604 2313 12-13 0.012985560239464178 2313 14-15 0.1354731515047618 2313 16-17 0.018131973098100218 2313 18-19 0.38069208448446545 2313 20-21 0.42202915691336074 2313 22-23 0.4968885338390834 2313 24-25 0.3024479327371239 2313 26-27 0.3882921508308854 2313 28-29 0.3795544044538204 2313 30-31 0.5562556748500143 2313 32-33 0.4275263819918891 2313 34-35 0.3968509018429458 2313 36-37 -0.30909897024950084 2313 38-39 0.1567303768461059 2313 40-41 0.3630134663760529 2313 42-43 0.49086078748866413 2313 44-45 0.43395622050384475 2313 46-47 0.2607133648480513 2313 48-49 -5.30019889638525 2313 50-51 -1.308390339172007 2313 52-53 0.08551427566057868 2313 54-55 0.2604810745207189 2313 56-57 0.3350942696455732 2313 58-59 0.3558434732527118 2313 60-61 0.6496563093801484 2313 62-63 0.6913549806676755 2313 64-65 0.7656408937994357 2313 66-67 0.8045284993474837 2313 68-69 0.8022702803550672 2313 70-71 0.7851983033777827 2313 72-73 0.568288567320252 2313 74-75 0.723552562599906 2313 76-77 0.7524293789860472 2313 78-79 0.639320881903565 2313 80-81 -0.2607140551392604 2313 82-83 0.1691201971239451 2313 84-85 0.5570806989097825 2313 86-87 0.7353484795288132 2313 88-89 0.7272412158753134 2313 90-91 0.7263030438527309 2313 92-93 0.8619312031139685 2313 94-95 0.6997201628868872 2313 96-97 0.6605981781787733 2313 98-99 0.8432363047893858 2313 100 0.9357379558020966 2314 1 0.08409742685960708 2314 2 0.17757696374761167 2314 3 0.18930935801802917 2314 4 0.06250278093480688 2314 5 0.2239729824519543 2314 6 0.29531740789844463 2314 7 0.12794491263517216 2314 8 0.2677943042688611 2314 9 0.41094187895278367 2314 10-11 0.31355517611233097 2314 12-13 0.19738040538130264 2314 14-15 0.2223206717547086 2314 16-17 0.4440802759313627 2314 18-19 0.5751044197720816 2314 20-21 0.608019496088609 2314 22-23 0.34566765923064224 2314 24-25 0.4191626279823595 2314 26-27 0.49326406788967603 2314 28-29 0.6368805764017154 2314 30-31 0.5288080048987709 2314 32-33 0.5143076493115544 2314 34-35 0.3730077732257655 2314 36-37 0.46643053872542595 2314 38-39 0.5408732812252879 2314 40-41 0.2983629361956588 2314 42-43 0.340462738000042 2314 44-45 0.5512251714752807 2314 46-47 0.6644781496803702 2314 48-49 -6.512844939712821 2314 50-51 -1.560422921173739 2314 52-53 -0.8391938382393533 2314 54-55 -0.4104871756938806 2314 56-57 -0.036478340182739544 2314 58-59 0.4526123392934309 2314 60-61 0.6691223323871256 2314 62-63 0.5347161458123892 2314 64-65 0.7296415122066264 2314 66-67 0.874339738662691 2314 68-69 0.8555950822502609 2314 70-71 0.602858947114207 2314 72-73 0.8104164321621852 2314 74-75 0.8297624886456632 2314 76-77 0.7674184373991828 2314 78-79 0.5782528232800956 2314 80-81 0.6097489707030377 2314 82-83 0.6440256048907571 2314 84-85 0.7143796738466754 2314 86-87 0.7832476739252776 2314 88-89 0.6876708379632994 2314 90-91 0.8517717148841761 2314 92-93 0.908093084885536 2314 94-95 0.9381674585849318 2314 96-97 0.9852315460773333 2314 98-99 0.9777104215012749 2314 100 -0.37268762414211665 2315 1 0.11549439897019198 2315 2 0.2258124334009466 2315 3 0.2955677202261455 2315 4 0.1420081281064327 2315 5 0.21931877173047098 2315 6 0.29216541137405017 2315 7 0.3523536063103663 2315 8 0.2638819850220955 2315 9 0.4319568094448698 2315 10-11 0.19125830339882555 2315 12-13 0.38623345010694976 2315 14-15 0.24336727040467565 2315 16-17 0.41555202118316714 2315 18-19 0.4416834662790734 2315 20-21 0.36583098628367594 2315 22-23 0.41752935533413904 2315 24-25 0.44342181250767965 2315 26-27 0.5088775035265698 2315 28-29 0.6030898038066894 2315 30-31 0.5807229895875068 2315 32-33 0.6371357326930394 2315 34-35 0.6766271208709966 2315 36-37 0.5138301293559451 2315 38-39 0.6278005264069648 2315 40-41 0.6963973668464831 2315 42-43 0.7250261165962684 2315 44-45 0.7541990541273051 2315 46-47 0.6162172273517115 2315 48-49 -4.763465357113066 2315 50-51 -0.8823789677116309 2315 52-53 0.259428565245976 2315 54-55 0.258114058862148 2315 56-57 0.5612890146007175 2315 58-59 0.7288785550359123 2315 60-61 0.5849334777558255 2315 62-63 0.2927671873719859 2315 64-65 0.5204178500291121 2315 66-67 -0.11622344988239064 2315 68-69 0.5412629390151622 2315 70-71 0.5852808071477824 2315 72-73 0.6804095508254946 2315 74-75 0.7456995893125153 2315 76-77 0.7263182446643732 2315 78-79 0.6884021028833089 2315 80-81 0.5494067242073868 2315 82-83 0.6157032459138634 2315 84-85 0.5879335973141302 2315 86-87 0.5831624547225438 2315 88-89 0.556879164397575 2315 90-91 0.6495485595659751 2315 92-93 0.7137948301660302 2315 94-95 0.3362117209223463 2315 96-97 0.47541420193573103 2315 98-99 0.46854580851781336 2315 100 0.5409864205934909 2316 1 0.23511137298912388 2316 2 0.2427325475045734 2316 3 0.18369168283215487 2316 4 -0.01915734038458794 2316 5 0.17032691266947353 2316 6 0.11547579034362343 2316 7 0.24098265996423862 2316 8 0.2842679849456644 2316 9 0.4455962487852574 2316 10-11 0.3860306428009821 2316 12-13 0.30013117323768057 2316 14-15 0.4399870302004061 2316 16-17 0.3992209217551945 2316 18-19 0.11256790550085327 2316 20-21 0.4368369746531613 2316 22-23 0.5771794448563483 2316 24-25 0.6269907624348932 2316 26-27 0.626658871478817 2316 28-29 0.670280928664198 2316 30-31 0.5953518354175955 2316 32-33 0.5630486135840158 2316 34-35 0.552358959372107 2316 36-37 0.46006760191524876 2316 38-39 0.5122311424778374 2316 40-41 0.08370866376935737 2316 42-43 0.11463415930576559 2316 44-45 0.3506687647752642 2316 46-47 0.39127347599280426 2316 48-49 -5.0826511814311175 2316 50-51 -1.0505913329764454 2316 52-53 0.20648469319554152 2316 54-55 0.49207630269464886 2316 56-57 0.38033071344318614 2316 58-59 0.2638033350663491 2316 60-61 0.4462292628018929 2316 62-63 0.3879338806247645 2316 64-65 0.4345999128111018 2316 66-67 0.48925553797953114 2316 68-69 0.17937843560404332 2316 70-71 -0.2072646074042055 2316 72-73 -0.04295039498116182 2316 74-75 0.11941055142811763 2316 76-77 0.18854224552154264 2316 78-79 0.26005754059171693 2316 80-81 0.12501574535141913 2316 82-83 0.11760484881705224 2316 84-85 0.26444097885986295 2316 86-87 0.30466030006192213 2316 88-89 0.20586398989154375 2316 90-91 0.203415574590057 2316 92-93 0.30968555413562626 2316 94-95 0.4983526312078119 2316 96-97 0.5618259286630582 2316 98-99 0.5831772127782706 2316 100 -0.25959077213584436 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 13.0 11 20.0 12 54.0 13 191.0 14 691.0 15 2000.0 16 4938.0 17 10128.0 18 17697.0 19 27163.0 20 39495.0 21 56060.0 22 70686.0 23 76170.0 24 78417.0 25 85673.0 26 100781.0 27 123056.0 28 151537.0 29 186927.0 30 228641.0 31 283939.0 32 357635.0 33 465703.0 34 648969.0 35 916824.0 36 1427904.0 37 2094671.0 38 1673132.0 39 218222.0 40 25.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.001904013002765 20.125402457109946 14.434563143950104 23.438130385937185 2 15.395128658357974 20.349372504455367 40.22640892557931 24.029089911607358 3 18.646028107621426 25.598719980554936 29.920550812325793 25.83470109949785 4 12.853047812296928 16.921694760043962 37.93115809088089 32.29409933677822 5 13.454916859048033 37.76535282469765 34.52398489877151 14.25574541748281 6 31.227844011571182 37.15976489618459 17.442358056178428 14.1700330360658 7 27.752931789739183 32.38199183898088 21.807029619693772 18.05804675158617 8 26.210241383357374 35.584009370280235 19.598675467922913 18.607073778439474 9 26.37584102296125 16.00711550041535 20.501262738119543 37.115780738503865 10-11 25.200575975751633 26.368489361357277 27.52904230792129 20.901892354969796 12-13 25.606619758187094 23.887977668110338 28.04560500860645 22.45979756509612 14-15 23.040031923477798 25.37591259129256 25.640201329734808 25.943854155494833 16-17 22.251083103236468 28.355071177265938 26.55364260912326 22.840203110374336 18-19 22.0210532081558 27.432562805941867 28.798209848252405 21.74817413764993 20-21 22.910465493080025 26.513205818500353 28.782719008164552 21.79360968025507 22-23 22.968884513549792 26.268390394314363 28.52840257532117 22.23432251681468 24-25 22.595275821699836 26.47811505300801 28.745193429461004 22.181415695831156 26-27 22.43234693350282 26.666649906139263 28.542174318970737 22.358828841387176 28-29 22.19500269089825 26.69596797475365 28.793611554542164 22.315417779805944 30-31 22.656068793986925 26.503146003849753 28.705332650911043 22.135452551252282 32-33 22.631038213655703 26.762216186070397 28.35129333691758 22.255452263356325 34-35 22.740903062569927 26.718889996893203 28.559691231138313 21.980515709398556 36-37 22.677089970441422 26.5331181316381 28.44566996294357 22.344121934976908 38-39 22.627267616340298 26.654121081783032 28.562201711090374 22.156409590786296 40-41 22.534359212364386 26.301988967264155 28.872142892983575 22.29150892738788 42-43 22.445911778606394 26.420429485446085 28.495180077421534 22.638478658525983 44-45 22.37187492376819 26.761912383812025 28.37800000256781 22.488212689851977 46-47 22.481748497814678 26.632379710421873 28.09421180783626 22.791659983927186 48-49 22.8335581778301 26.733910518115668 27.89339572138814 22.539135582666095 50-51 22.61390179462647 26.760975788788144 27.81347085614542 22.811651560439966 52-53 22.46707767828548 27.01270196892449 27.671314257392197 22.848906095397826 54-55 22.44885927975904 26.9315813938385 27.711343402727124 22.90821592367534 56-57 22.64962031516481 26.841418356673557 27.48545238240057 23.02350894576106 58-59 22.343701168568096 26.845937123223962 27.862214008630122 22.948147699577824 60-61 22.532979139347987 27.08907824668949 27.595494548942156 22.78244806502037 62-63 22.51058254680733 26.884785702925633 27.603097348517615 23.00153440174942 64-65 22.176890532878208 26.784667092430368 27.708827919826522 23.329614454864902 66-67 22.390224245915796 26.737138160480306 27.538015284608125 23.33462230899577 68-69 22.1345299589817 26.786307828791145 27.695216814826946 23.383945397400215 70-71 22.237169753373763 26.505316798128607 27.712112797234 23.54540065126363 72-73 22.34620061235865 26.770980367734072 27.501544379523256 23.381274640384017 74-75 22.313655703761086 26.893328664890408 27.44156399614078 23.351451635207727 76-77 22.218079629582668 26.813136829995383 27.37793466487638 23.590848875545568 78-79 22.17694059096461 26.724428697960906 27.39097293107581 23.707657779998677 80-81 22.01642254697813 27.02654414794511 27.342974232029576 23.614059073047184 82-83 22.365482283470886 26.658182126334445 27.624080116259137 23.35225547393553 84-85 22.34290492332844 26.746769785003323 27.236859747455384 23.673465544212856 86-87 21.91079588245227 26.946287998489915 27.534623753394 23.608292365663818 88-89 22.08811303327068 26.549370829874448 27.481715310082848 23.88080082677202 90-91 22.146262786488172 26.477168689834112 27.53290598638134 23.843662537296375 92-93 22.176100147109633 26.57575236534718 27.3751025963689 23.87304489117429 94-95 21.75758725406367 26.39649939529641 27.61076389512993 24.23514945550999 96-97 21.86336623769777 26.326815261431303 27.564989659234573 24.244828841636348 98-99 21.65538446295271 26.457178316490946 27.480754021530373 24.40668319902597 100 21.705355757345504 26.284959038542834 27.37936569939481 24.630319504716855 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2412.0 1 1703.0 2 1430.5 3 1870.0 4 1509.0 5 1349.5 6 2175.0 7 4300.0 8 7080.0 9 8612.0 10 7645.5 11 6179.0 12 6145.0 13 6799.0 14 7841.5 15 8864.0 16 9814.0 17 10720.5 18 11654.5 19 12665.0 20 13674.0 21 15142.5 22 17104.5 23 19914.0 24 23535.0 25 27732.5 26 34014.0 27 41990.5 28 51217.5 29 64152.0 30 81918.0 31 100357.0 32 123587.5 33 151799.5 34 178792.0 35 204277.0 36 234823.5 37 269207.5 38 286704.5 39 290399.5 40 287035.0 41 281868.5 42 285918.0 43 299052.0 44 315921.0 45 340709.0 46 360332.0 47 368381.5 48 377827.5 49 381250.5 50 376101.0 51 371052.5 52 364229.0 53 351733.0 54 337673.0 55 311349.0 56 278681.5 57 244899.0 58 211965.5 59 185190.5 60 154711.0 61 124076.0 62 98954.5 63 77355.5 64 57312.5 65 40315.0 66 28916.5 67 21883.0 68 16661.5 69 12330.5 70 9393.5 71 7026.5 72 5174.0 73 3894.0 74 2923.5 75 2026.5 76 1344.5 77 787.0 78 442.0 79 292.0 80 180.5 81 104.0 82 62.0 83 41.0 84 28.0 85 17.0 86 13.5 87 9.5 88 6.0 89 1.5 90 1.0 91 1.0 92 1.0 93 1.5 94 1.0 95 0.5 96 1.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0309285023105442 2 0.0022145278375242648 3 7.488741479550654E-5 4 0.020583340866650656 5 4.921172972276144E-4 6 5.349101056821896E-5 7 3.209460634093137E-5 8 0.003915541973593627 9 0.010794485932666586 10-11 0.0010323765039666258 12-13 1.5512393064783496E-4 14-15 1.3372752642054741E-4 16-17 3.58389770807067E-4 18-19 1.0698202113643792E-5 20-21 8.558561690915034E-5 22-23 2.781532549547386E-4 24-25 4.279280845457517E-5 26-27 6.418921268186274E-5 28-29 0.0012249441420122142 30-31 0.00287781636857018 32-33 0.009655127407563522 34-35 0.018545333364001514 36-37 0.02164781197695821 38-39 0.021171741982901063 40-41 0.004856983759594281 42-43 0.00516188251983313 44-45 0.00933953044521103 46-47 0.012281536026463074 48-49 1.1873025178753585 50-51 0.018352765725955927 52-53 0.01932095301724069 54-55 0.00987978965195004 56-57 0.007825734846130434 58-59 0.008537165286687746 60-61 0.005450733976901512 62-63 0.007761545633448571 64-65 0.019315603916183866 66-67 0.029264931881872593 68-69 0.02947354682308865 70-71 0.02626408618899551 72-73 0.023407666224652617 74-75 0.009869091449836397 76-77 0.014613744087237418 78-79 0.008542514387744567 80-81 0.0029580528844225084 82-83 0.014410478247078187 84-85 0.02400676554301667 86-87 0.02343976083099355 88-89 0.03286487689311372 90-91 0.020893588727946326 92-93 0.01362950949278219 94-95 0.006814754746391095 96-97 0.004043920398957354 98-99 0.015528440367953963 100 0.034394719795364785 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9347365.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.53766249046588 #Duplication Level Percentage of deduplicated Percentage of total 1 70.71935754312443 37.154297381432535 2 15.977995608666617 16.788930811245454 3 5.882555826273256 9.271671977462038 4 2.7003676172591184 5.674840099029724 5 1.4618781540208534 3.840183052906644 6 0.8387066986865729 2.643821367845281 7 0.5434040234806415 1.9984424027110994 8 0.35597091658188207 1.496150389744056 9 0.2568699796366613 1.214581346467538 >10 1.1616326605553138 10.477956903396304 >50 0.05851913219200064 2.1215275901888186 >100 0.03825721367196032 3.913031407656324 >500 0.00314231461093238 1.1302501901361817 >1k 0.0012202829453474776 1.0074854063623642 >5k 3.0507073589162214E-5 0.10422815493702262 >10k+ 9.152122076748664E-5 1.1626015184786727 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30913 0.33071352193907055 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26110 0.2793300571872394 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15514 0.16597190759106978 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0698202113643792E-5 2 0.0 0.0 0.0 0.0 1.0698202113643792E-5 3 0.0 0.0 0.0 0.0 1.0698202113643792E-5 4 0.0 0.0 0.0 0.0 2.1396404227287585E-5 5 0.0 0.0 0.0 0.0 2.1396404227287585E-5 6 0.0 0.0 0.0 0.0 4.279280845457517E-5 7 0.0 0.0 0.0 0.0 6.418921268186275E-5 8 0.0 0.0 0.0 0.0 8.558561690915034E-5 9 0.0 0.0 0.0 0.0 9.628381902279412E-5 10-11 0.0 0.0 0.0 1.0698202113643792E-5 9.628381902279412E-5 12-13 0.0 4.279280845457517E-5 0.0 1.0698202113643792E-5 9.628381902279412E-5 14-15 0.0 4.279280845457517E-5 0.0 1.0698202113643792E-5 1.8186943593194446E-4 16-17 5.349101056821896E-6 4.279280845457517E-5 0.0 1.0698202113643792E-5 2.2466224438651963E-4 18-19 1.0698202113643792E-5 4.279280845457517E-5 0.0 1.604730317046569E-5 2.3001134544334154E-4 20-21 1.0698202113643792E-5 4.279280845457517E-5 0.0 5.3491010568218955E-5 2.460586486138072E-4 22-23 1.0698202113643792E-5 4.279280845457517E-5 0.0 2.0326584015923203E-4 2.728041538979167E-4 24-25 1.0698202113643792E-5 6.418921268186275E-5 0.0 7.22128642670956E-4 3.1024786129566997E-4 26-27 1.0698202113643792E-5 7.488741479550654E-5 0.0 0.0018721853698876636 3.2094606340931373E-4 28-29 1.0698202113643792E-5 7.488741479550654E-5 0.0 0.005370497461049183 3.2094606340931373E-4 30-31 1.0698202113643792E-5 7.488741479550654E-5 0.0 0.01622382350534081 3.2629516446613564E-4 32-33 1.0698202113643792E-5 7.488741479550654E-5 0.0 0.04159995891890388 3.4234246763660135E-4 34-35 1.0698202113643792E-5 7.488741479550654E-5 0.0 0.08161123482393166 3.4234246763660135E-4 36-37 1.0698202113643792E-5 7.488741479550654E-5 0.0 0.14664560547277228 3.4234246763660135E-4 38-39 1.0698202113643792E-5 8.023651585232843E-5 0.0 0.2349164711124472 3.4234246763660135E-4 40-41 1.0698202113643792E-5 1.1768022325008171E-4 0.0 0.32831712466561436 3.476915686934232E-4 42-43 1.0698202113643792E-5 1.1768022325008171E-4 0.0 0.4197386108277573 3.530406697502451E-4 44-45 1.0698202113643792E-5 1.1768022325008171E-4 0.0 0.533727954348632 3.58389770807067E-4 46-47 1.0698202113643792E-5 1.1768022325008171E-4 0.0 0.6732699536179447 3.6908797292071083E-4 48-49 1.0698202113643792E-5 1.1768022325008171E-4 0.0 0.8204183745900583 3.744370739775327E-4 50-51 1.0698202113643792E-5 1.230293243069036E-4 0.0 0.9756011453495184 3.744370739775327E-4 52-53 1.0698202113643792E-5 1.283784253637255E-4 0.0 1.1292112803982728 4.0118257926164216E-4 54-55 1.0698202113643792E-5 1.283784253637255E-4 0.0 1.298590565362538 4.2792808454575164E-4 56-57 1.0698202113643792E-5 1.283784253637255E-4 0.0 1.4979622599524038 4.2792808454575164E-4 58-59 1.0698202113643792E-5 1.4977482959101308E-4 0.0 1.721410258399025 4.2792808454575164E-4 60-61 1.0698202113643792E-5 1.4977482959101308E-4 0.0 1.9489931119625692 4.2792808454575164E-4 62-63 1.0698202113643792E-5 1.4977482959101308E-4 0.0 2.1786246712308763 4.60022690886683E-4 64-65 1.0698202113643792E-5 1.9256763804558825E-4 0.0 2.4152207600751656 4.60022690886683E-4 66-67 1.0698202113643792E-5 1.9256763804558825E-4 0.0 2.6846121874988302 4.6537179194350493E-4 68-69 1.0698202113643792E-5 1.9256763804558825E-4 0.0 2.985461678237664 4.7606999405714874E-4 70-71 1.0698202113643792E-5 1.9256763804558825E-4 0.0 3.305022324473261 4.8141909511397065E-4 72-73 1.0698202113643792E-5 1.9791673910241015E-4 0.0 3.6181533512385577 5.723538130799428E-4 74-75 1.0698202113643792E-5 2.0326584015923203E-4 0.0 3.9305194565527293 5.777029141367648E-4 76-77 1.0698202113643792E-5 2.0326584015923203E-4 0.0 4.269887824001737 5.777029141367648E-4 78-79 1.0698202113643792E-5 2.0326584015923203E-4 0.0 4.64328182327319 5.830520151935867E-4 80-81 1.604730317046569E-5 2.086149412160539E-4 0.0 5.038393172835339 6.311939247049838E-4 82-83 2.1396404227287585E-5 2.1396404227287582E-4 0.0 5.437628679312298 6.739867331595589E-4 84-85 2.1396404227287585E-5 2.1396404227287582E-4 0.0 5.828423304321593 6.793358342163807E-4 86-87 2.674550528410948E-5 2.1396404227287582E-4 0.0 6.233494680051544 6.953831373868464E-4 88 3.209460634093138E-5 2.1396404227287582E-4 0.0 6.555034493678165 7.060813395004902E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 16470 0.0 54.189816 1 GTATCAA 29505 0.0 39.90302 1 CAACGCA 36610 0.0 31.213406 5 ATCAACG 36605 0.0 31.18423 3 TATCAAC 38040 0.0 30.572914 2 TCAACGC 38715 0.0 29.654325 4 AACGCAG 39245 0.0 29.452354 6 GTACTAG 3735 0.0 27.137129 1 ACGCAGA 44345 0.0 25.71603 7 CGCAGAG 45290 0.0 25.127663 8 GTACATG 38810 0.0 24.70162 1 GTGGTAT 7505 0.0 24.44707 1 TACATGG 40080 0.0 23.597372 2 ACATGGG 40070 0.0 22.97275 3 GCAGAGT 49540 0.0 22.78211 9 TGGTATC 7775 0.0 22.627245 2 GAGTACT 27700 0.0 21.775314 12-13 CATGGGG 27165 0.0 20.604595 4 CAGAGTA 48915 0.0 20.366846 10-11 GTACTTT 29410 0.0 20.365744 14-15 >>END_MODULE