FastQCFastQC Report
Mon 6 Jun 2016
ERR1211298_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211298_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4308178
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182670.42400755029156173No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141700.32890934404288774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83200.19312108274077813No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69850.16213350516157873No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78150.049.6023521
TACCTGG43850.041.409172
GTATCAA137950.041.2636571
GTACCTG52250.035.8344571
ACCTGGG52550.033.7138523
ATCAACG167500.033.3304633
CAACGCA169650.033.1577535
TATCAAC172450.032.6247182
TCAACGC174100.032.282874
AACGCAG180100.031.624866
ACGCAGA200900.028.1180027
CGCAGAG206500.027.2391578
GTACATG148150.026.0385171
ATAACGC6550.025.1111413
GTGGTAT38050.024.851091
GCAGAGT220150.024.654899
TACATGG152800.024.474992
ACATGGG153500.023.6652373
TAACGCA6200.023.4968574
TGGTATC40600.022.4778352
Produced by FastQC (version 0.11.5)