##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211298_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4308178 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.283623610723605 31.0 31.0 34.0 28.0 34.0 2 31.279538821283616 31.0 31.0 34.0 28.0 34.0 3 31.32733095057818 31.0 31.0 34.0 28.0 34.0 4 34.93099333407301 37.0 35.0 37.0 32.0 37.0 5 34.986077873291215 37.0 35.0 37.0 32.0 37.0 6 35.04617938256033 37.0 35.0 37.0 32.0 37.0 7 34.988086611091745 37.0 35.0 37.0 32.0 37.0 8 34.98719899688453 37.0 35.0 37.0 32.0 37.0 9 36.53341830351485 39.0 37.0 39.0 32.0 39.0 10-11 36.460279612402275 39.0 37.0 39.0 32.0 39.0 12-13 36.37783106918981 39.0 37.0 39.0 32.0 39.0 14-15 37.49377637135699 40.0 37.0 41.0 31.5 41.0 16-17 37.48885491732236 40.0 37.0 41.0 31.5 41.0 18-19 37.49986432779704 40.0 37.0 41.0 31.0 41.0 20-21 37.45974110169078 40.0 37.0 41.0 31.0 41.0 22-23 37.32395249221365 40.0 37.0 41.0 31.0 41.0 24-25 37.210359576600595 40.0 36.5 41.0 31.0 41.0 26-27 37.06115996135722 40.0 36.0 41.0 30.0 41.0 28-29 36.91191775270195 39.5 36.0 41.0 30.0 41.0 30-31 36.71103259893161 39.0 36.0 41.0 30.0 41.0 32-33 36.55566471487482 39.0 35.0 41.0 30.0 41.0 34-35 36.37605700135882 39.0 35.0 41.0 29.0 41.0 36-37 36.26074398504426 39.0 35.0 41.0 28.5 41.0 38-39 36.09851751714994 39.0 35.0 40.5 28.0 41.0 40-41 35.92810580249934 39.0 35.0 40.0 27.0 41.0 42-43 35.76963219718405 38.5 35.0 40.0 27.0 41.0 44-45 35.62454522538298 38.0 34.5 40.0 26.0 41.0 46-47 35.44509221763818 38.0 34.0 40.0 25.5 41.0 48-49 35.2023616480099 38.0 34.0 40.0 24.0 41.0 50-51 34.53377274569435 37.5 33.5 39.5 23.5 40.5 52-53 34.77041791216612 38.0 33.5 40.0 24.0 40.5 54-55 35.13167619815151 38.0 34.0 40.0 24.5 41.0 56-57 35.037638068807745 38.0 34.0 40.0 24.5 41.0 58-59 34.82507907054908 38.0 34.0 40.0 24.0 41.0 60-61 34.56011822167051 37.5 33.0 40.0 23.5 41.0 62-63 34.29454087087395 37.0 33.0 40.0 23.0 41.0 64-65 34.0234485436767 36.5 33.0 40.0 23.0 41.0 66-67 33.80290368689502 36.0 32.5 39.0 22.5 41.0 68-69 33.46947526309266 36.0 32.0 39.0 22.0 41.0 70-71 33.11850787038047 35.0 32.0 39.0 22.0 40.5 72-73 32.74902801137743 35.0 31.5 38.0 21.0 40.0 74-75 32.38135738588331 35.0 31.0 37.0 20.0 39.5 76-77 32.01933253454244 35.0 31.0 37.0 20.0 39.0 78-79 31.6500448681554 35.0 30.5 36.0 19.5 39.0 80-81 31.310014697628556 35.0 30.0 36.0 19.0 37.5 82-83 30.99040510860972 34.0 30.0 35.0 18.0 37.0 84-85 30.680077517688453 34.0 29.5 35.0 18.0 37.0 86-87 30.420503168624883 34.0 29.0 35.0 17.5 36.0 88-89 30.149880645600064 34.0 29.0 35.0 17.0 36.0 90-91 29.886122393271588 34.0 29.0 35.0 15.0 36.0 92-93 29.650174969557895 34.0 29.0 35.0 13.5 35.0 94-95 29.420338597894514 34.0 27.5 35.0 11.5 35.0 96-97 29.213148922816096 34.0 27.0 35.0 10.5 35.0 98-99 28.970704785178327 34.0 27.0 35.0 10.0 35.0 100 27.349267602220706 32.0 24.0 34.0 8.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4731020224265592 1101 2 -0.46267878080322333 1101 3 -0.4777400239413403 1101 4 -0.3607590391053108 1101 5 -0.4504310351146401 1101 6 -0.31780172292358344 1101 7 -0.4737876355864472 1101 8 -0.6338446747260704 1101 9 -0.571257137337696 1101 10-11 -0.6007853159160632 1101 12-13 -0.5977939539133956 1101 14-15 -0.663031257932893 1101 16-17 -0.8674108308932347 1101 18-19 -0.8513702034883082 1101 20-21 -0.8584296886756917 1101 22-23 -0.8491274327081371 1101 24-25 -0.7179928484392022 1101 26-27 -0.8487712626624528 1101 28-29 -0.8746590625241026 1101 30-31 -0.803292526019348 1101 32-33 -0.8138423223707747 1101 34-35 -0.8542916949251733 1101 36-37 -0.8727070612437373 1101 38-39 -0.8440625432304145 1101 40-41 -0.9182255818032132 1101 42-43 -0.9533160412103641 1101 44-45 -1.1112954433755746 1101 46-47 -1.1769041447354809 1101 48-49 -1.1082576664084698 1101 50-51 -1.0802915601130252 1101 52-53 -0.8814286223699952 1101 54-55 -0.7362511549081105 1101 56-57 -0.8300266526852127 1101 58-59 -0.876497618217094 1101 60-61 -0.8315256742831423 1101 62-63 -0.8003105207302852 1101 64-65 -0.7950444227241675 1101 66-67 -0.9104037858655971 1101 68-69 -0.8611061375052174 1101 70-71 -0.9977481568198954 1101 72-73 -1.0015112993506534 1101 74-75 -0.8471782474223417 1101 76-77 -0.9600917632725228 1101 78-79 -0.9584317796102333 1101 80-81 -1.0487270912796944 1101 82-83 -1.182675254801179 1101 84-85 -1.0476271419125034 1101 86-87 -1.0916391746418448 1101 88-89 -1.1855496722926588 1101 90-91 -1.1933589165219551 1101 92-93 -1.1954858120636018 1101 94-95 -1.1941034362631697 1101 96-97 -1.3595116501495035 1101 98-99 -1.3569071694361483 1101 100 -1.3370950732162683 1102 1 -0.26413343520046695 1102 2 -0.2273118766870077 1102 3 -0.3173071610402509 1102 4 0.004275232038423837 1102 5 -0.09325977413475783 1102 6 -0.170576666501006 1102 7 -0.24443352993645817 1102 8 -0.19686297240664885 1102 9 -0.31288023142020194 1102 10-11 -0.28942128575172177 1102 12-13 -0.2270903125147754 1102 14-15 -0.43201953462757814 1102 16-17 -0.4133690516982469 1102 18-19 -0.3431096954264703 1102 20-21 -0.49584320055014786 1102 22-23 -0.4495187133712619 1102 24-25 -0.5654554842600774 1102 26-27 -0.4553575737760056 1102 28-29 -0.610932451948635 1102 30-31 -0.5011980969734253 1102 32-33 -0.5512612256318405 1102 34-35 -0.5059918359738234 1102 36-37 -0.6410470470703089 1102 38-39 -0.6899694679523236 1102 40-41 -0.6582427906106858 1102 42-43 -0.5648345361596014 1102 44-45 -0.5926951595330863 1102 46-47 -0.6291259413948183 1102 48-49 -0.6626700021503709 1102 50-51 -0.6019410158946172 1102 52-53 -0.32402733928230987 1102 54-55 -0.2972289062448894 1102 56-57 -0.31773717386712264 1102 58-59 -0.4470831788994829 1102 60-61 -0.5035299954951071 1102 62-63 -0.4838201544837304 1102 64-65 -0.5889728487945476 1102 66-67 -0.5394262121640949 1102 68-69 -0.5218059987003869 1102 70-71 -0.7326648178250821 1102 72-73 -0.6361647640800783 1102 74-75 -0.5923328555620273 1102 76-77 -0.6725457417107137 1102 78-79 -0.6388186941851153 1102 80-81 -0.6719293131542656 1102 82-83 -0.7639801698136068 1102 84-85 -0.800599806024131 1102 86-87 -0.9242017162848519 1102 88-89 -1.0255328499198377 1102 90-91 -1.0014551586943163 1102 92-93 -0.8471895369819649 1102 94-95 -0.8416878924904054 1102 96-97 -0.988263494284201 1102 98-99 -1.1081165696459436 1102 100 -1.0792585525242515 1103 1 -0.451720442471796 1103 2 -0.41337371737020234 1103 3 -0.3778021615358398 1103 4 -0.26817922950543505 1103 5 -0.2664645557284615 1103 6 -0.3547198452351097 1103 7 -0.4006678567215616 1103 8 -0.3860674182493966 1103 9 -0.6556474324805563 1103 10-11 -0.5568148522513994 1103 12-13 -0.5860368105400013 1103 14-15 -0.6026980730274545 1103 16-17 -0.5937759592384495 1103 18-19 -0.6730621039187099 1103 20-21 -0.8070767489764208 1103 22-23 -0.7837885577262824 1103 24-25 -0.7627699677170483 1103 26-27 -0.7185539556427116 1103 28-29 -0.7306954588008736 1103 30-31 -0.8120183562100749 1103 32-33 -0.8739424530158999 1103 34-35 -1.024469002973909 1103 36-37 -0.9867233456422042 1103 38-39 -0.9536217031815823 1103 40-41 -0.9846550802863732 1103 42-43 -0.9689945292302724 1103 44-45 -0.99517409101162 1103 46-47 -0.9576390557736829 1103 48-49 -1.1296919209135226 1103 50-51 -0.978413237711699 1103 52-53 -0.8783666078869459 1103 54-55 -0.7986973702110447 1103 56-57 -0.8632280801655199 1103 58-59 -0.7927329864826334 1103 60-61 -0.7728176676902478 1103 62-63 -0.9269080602162987 1103 64-65 -0.9563519643110325 1103 66-67 -0.7616402903089394 1103 68-69 -0.7021151277034647 1103 70-71 -0.7113296005558141 1103 72-73 -0.8764672762890022 1103 74-75 -0.7991980101786211 1103 76-77 -0.70362718721384 1103 78-79 -0.8530073421713666 1103 80-81 -0.8180194515711676 1103 82-83 -1.0034572942495181 1103 84-85 -0.8472518510540503 1103 86-87 -0.9503289748276771 1103 88-89 -0.9346133209117582 1103 90-91 -0.9670156083029511 1103 92-93 -0.9607334865740782 1103 94-95 -1.1295758106052745 1103 96-97 -1.1454287263199632 1103 98-99 -1.279694164579677 1103 100 -1.4391154178425758 1104 1 -0.33882022542664814 1104 2 -0.32781418317569333 1104 3 -0.3752674523965638 1104 4 -0.22174834205250704 1104 5 -0.17231392394508305 1104 6 -0.28307700341036224 1104 7 -0.25213390115985135 1104 8 -0.24682018129595917 1104 9 -0.2953986208746642 1104 10-11 -0.30451439010841597 1104 12-13 -0.4093860954529944 1104 14-15 -0.5609597438678477 1104 16-17 -0.47206663113858127 1104 18-19 -0.5465839626522424 1104 20-21 -0.46371458261462806 1104 22-23 -0.4332161406589705 1104 24-25 -0.6035664530722684 1104 26-27 -0.5791531409473194 1104 28-29 -0.6118330505025185 1104 30-31 -0.7063380730289452 1104 32-33 -0.6977739022629095 1104 34-35 -0.6880690987802112 1104 36-37 -0.6938338598150793 1104 38-39 -0.7265828927974809 1104 40-41 -0.7885577349242823 1104 42-43 -0.6735007721619226 1104 44-45 -0.6581909901168075 1104 46-47 -0.7446061509770701 1104 48-49 -0.7122426272002613 1104 50-51 -0.7323873910832432 1104 52-53 -0.6073147804898156 1104 54-55 -0.5480787872615522 1104 56-57 -0.5215243682975839 1104 58-59 -0.6753239063570575 1104 60-61 -0.5722069024672578 1104 62-63 -0.5494237960608714 1104 64-65 -0.6558384151514858 1104 66-67 -0.6758163204474883 1104 68-69 -0.5944940832381178 1104 70-71 -0.7020269332158833 1104 72-73 -0.5802420135550079 1104 74-75 -0.515820233995715 1104 76-77 -0.5586043022078968 1104 78-79 -0.4815707089030532 1104 80-81 -0.6074775393011578 1104 82-83 -0.642444865915575 1104 84-85 -0.712885709186093 1104 86-87 -0.717338937943385 1104 88-89 -0.69284891842387 1104 90-91 -0.7097694729511907 1104 92-93 -0.8014303635561006 1104 94-95 -0.8259804538506543 1104 96-97 -0.8457226529005446 1104 98-99 -0.9338595888116679 1104 100 -0.9161921064643828 1105 1 -0.3490738117026879 1105 2 -0.2725184836307655 1105 3 -0.32765227891029625 1105 4 -0.22140591497308293 1105 5 -0.24681367076761518 1105 6 -0.37549770786300485 1105 7 -0.3026738714261725 1105 8 -0.32102384607522794 1105 9 -0.5215810163180308 1105 10-11 -0.4767069434122888 1105 12-13 -0.47109066588307513 1105 14-15 -0.47604025673132355 1105 16-17 -0.5215708202036566 1105 18-19 -0.5966090780190072 1105 20-21 -0.5975690648677201 1105 22-23 -0.7072969404973932 1105 24-25 -0.586779026217485 1105 26-27 -0.5664950770527 1105 28-29 -0.6578602487649619 1105 30-31 -0.5578379522080468 1105 32-33 -0.5805576927525848 1105 34-35 -0.5881697423893186 1105 36-37 -0.6261652498949672 1105 38-39 -0.593968494426889 1105 40-41 -0.7524633505259999 1105 42-43 -0.671541328318547 1105 44-45 -0.8462010637871984 1105 46-47 -0.7321451040811553 1105 48-49 -0.696206536357515 1105 50-51 -0.6734215289583148 1105 52-53 -0.5989065968873746 1105 54-55 -0.6188956215596235 1105 56-57 -0.6554466478743208 1105 58-59 -0.5886821648198861 1105 60-61 -0.5090051242750064 1105 62-63 -0.500119760885589 1105 64-65 -0.5292142644900508 1105 66-67 -0.5951230351348968 1105 68-69 -0.6436020121249939 1105 70-71 -0.7664590810472447 1105 72-73 -0.7042906754382194 1105 74-75 -0.618593083818773 1105 76-77 -0.6801045942423372 1105 78-79 -0.690006193577009 1105 80-81 -0.7210278362129543 1105 82-83 -0.7868081357137058 1105 84-85 -0.6204426417356537 1105 86-87 -0.683178801069694 1105 88-89 -0.711284225003368 1105 90-91 -0.8014461270743389 1105 92-93 -0.8221495292321173 1105 94-95 -0.884261158246634 1105 96-97 -0.9887398928492424 1105 98-99 -0.8567289010715662 1105 100 -0.8923748054778606 1106 1 -0.6143508442582899 1106 2 -0.5573709957595661 1106 3 -0.6082435858454076 1106 4 -0.4508631563010894 1106 5 -0.42591388317659806 1106 6 -0.46766019660202574 1106 7 -0.5510901599717926 1106 8 -0.43977993940293914 1106 9 -0.7034620838178043 1106 10-11 -0.6726045903836066 1106 12-13 -0.7287781354061096 1106 14-15 -0.8607989121907309 1106 16-17 -0.9286750328440618 1106 18-19 -0.8189061695862918 1106 20-21 -0.8031171544266158 1106 22-23 -0.7373384800905924 1106 24-25 -0.8355108040889334 1106 26-27 -0.7862077877053721 1106 28-29 -0.7809786109842634 1106 30-31 -0.7960231364749077 1106 32-33 -0.8220931534817169 1106 34-35 -0.8201296739556057 1106 36-37 -0.916651641669695 1106 38-39 -0.984139297696494 1106 40-41 -0.9586198389178904 1106 42-43 -1.0076577648066092 1106 44-45 -1.0392978545106288 1106 46-47 -1.049036428372645 1106 48-49 -1.0555806489094763 1106 50-51 -1.073125776311464 1106 52-53 -0.8637536872149099 1106 54-55 -0.950106815595646 1106 56-57 -0.8361598130987531 1106 58-59 -0.8284716071843192 1106 60-61 -0.7748143529080664 1106 62-63 -0.9034750624319656 1106 64-65 -0.8506538188444281 1106 66-67 -0.7505620440031748 1106 68-69 -0.8720226976191938 1106 70-71 -0.9511755853078157 1106 72-73 -0.8494769589422226 1106 74-75 -0.8280584578486767 1106 76-77 -0.7889160792042773 1106 78-79 -0.74316659128597 1106 80-81 -0.8844763332334473 1106 82-83 -0.7573342983842757 1106 84-85 -0.6344451377478144 1106 86-87 -0.7304641481558924 1106 88-89 -0.7493045402282164 1106 90-91 -0.7118073876431374 1106 92-93 -0.7332073259641589 1106 94-95 -0.7576048062262686 1106 96-97 -0.697914638495682 1106 98-99 -0.5420582672332479 1106 100 -0.649326761376134 1107 1 -0.5123981228973378 1107 2 -0.45988241092949167 1107 3 -0.493976638110162 1107 4 -0.4307842807828379 1107 5 -0.3102739102463019 1107 6 -0.3464953903293164 1107 7 -0.3495097870632762 1107 8 -0.4264711036335953 1107 9 -0.5286037408259787 1107 10-11 -0.557615472715959 1107 12-13 -0.6133148000762461 1107 14-15 -0.646358656117549 1107 16-17 -0.6828833606675104 1107 18-19 -0.6433046231905237 1107 20-21 -0.6389439083178914 1107 22-23 -0.6268405375437069 1107 24-25 -0.734356483437395 1107 26-27 -0.6648935068805244 1107 28-29 -0.7376045434500185 1107 30-31 -0.7915172752615973 1107 32-33 -0.7624122337340538 1107 34-35 -0.6399273356932014 1107 36-37 -0.7962289497898354 1107 38-39 -0.7681521169128729 1107 40-41 -0.739571416817185 1107 42-43 -0.832930881011869 1107 44-45 -0.6748980940930736 1107 46-47 -0.7713925194450866 1107 48-49 -0.6449110782132124 1107 50-51 -0.8722368009931287 1107 52-53 -0.6671579670285439 1107 54-55 -0.4804290539801812 1107 56-57 -0.5507901171662084 1107 58-59 -0.5334191712998475 1107 60-61 -0.580479979828425 1107 62-63 -0.5355633034456773 1107 64-65 -0.5116706129513275 1107 66-67 -0.5277801807051858 1107 68-69 -0.502314350349117 1107 70-71 -0.5516096651323323 1107 72-73 -0.5761633626386384 1107 74-75 -0.5693362101297623 1107 76-77 -0.45957289291337133 1107 78-79 -0.4767784210579755 1107 80-81 -0.4656405172140907 1107 82-83 -0.5118375304136471 1107 84-85 -0.44853386103787685 1107 86-87 -0.5688595369717468 1107 88-89 -0.5556277431654344 1107 90-91 -0.5343634522681633 1107 92-93 -0.41273870893046904 1107 94-95 -0.45427373907318014 1107 96-97 -0.5494488217762488 1107 98-99 -0.5449982549429784 1107 100 -0.663344574486274 1108 1 -0.5482565928209517 1108 2 -0.5365414122911325 1108 3 -0.5968072597376484 1108 4 -0.4782104662837696 1108 5 -0.5629770786150772 1108 6 -0.4756026239838107 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2311 26-27 1.0331041634159703 2311 28-29 1.020349452674111 2311 30-31 1.0720092469533284 2311 32-33 1.1368225163234413 2311 34-35 1.123171377120208 2311 36-37 1.1240530343970434 2311 38-39 1.1990003904777495 2311 40-41 1.2675626774618678 2311 42-43 1.3027221400489282 2311 44-45 1.3260264086476141 2311 46-47 1.1877810222571483 2311 48-49 1.1581504789021366 2311 50-51 1.250211722616342 2311 52-53 1.1754229502661957 2311 54-55 1.154620505117741 2311 56-57 1.0468940735064578 2311 58-59 1.032468172043572 2311 60-61 1.0613321510936728 2311 62-63 0.9870070864341542 2311 64-65 0.9307116427909747 2311 66-67 0.9567104769292669 2311 68-69 1.0201061234999713 2311 70-71 1.0890955434275256 2311 72-73 0.9603463573900299 2311 74-75 1.0064823612382767 2311 76-77 1.088431778725635 2311 78-79 1.087742612390489 2311 80-81 1.1421259272723603 2311 82-83 1.069646850871127 2311 84-85 1.069787880416726 2311 86-87 1.1504162218650222 2311 88-89 1.0431069694808741 2311 90-91 1.185987662469362 2311 92-93 1.1083217683159532 2311 94-95 1.085852091747455 2311 96-97 1.0208472958005324 2311 98-99 1.1581015913036943 2311 100 1.2692722264767795 2312 1 0.420131055455812 2312 2 0.5151818429762187 2312 3 0.4962888354023569 2312 4 0.38490974483597284 2312 5 0.3720352819779009 2312 6 0.44884105011799136 2312 7 0.40993546632551414 2312 8 0.3948291649168496 2312 9 0.5519282113480557 2312 10-11 0.559714935471149 2312 12-13 0.563181630132199 2312 14-15 0.7210202285515592 2312 16-17 0.6961219019218063 2312 18-19 0.6550738567841208 2312 20-21 0.6683246022084077 2312 22-23 0.7548713082933745 2312 24-25 0.712945847912998 2312 26-27 0.741075145937792 2312 28-29 0.7607991725955898 2312 30-31 0.7918240195674429 2312 32-33 0.8031754461449196 2312 34-35 0.8182640284764773 2312 36-37 0.803172381515374 2312 38-39 0.8251824082864516 2312 40-41 0.7992167950686095 2312 42-43 0.9120554504255693 2312 44-45 0.8618394674564414 2312 46-47 0.8453255773452995 2312 48-49 0.8375717120257562 2312 50-51 0.859909849855292 2312 52-53 0.7889134842319336 2312 54-55 0.7145738282601783 2312 56-57 0.7007371725802827 2312 58-59 0.6984974663264651 2312 60-61 0.6839125827716188 2312 62-63 0.6659701928519084 2312 64-65 0.6872092899922677 2312 66-67 0.6784640000432418 2312 68-69 0.744696770795052 2312 70-71 0.7014272266659134 2312 72-73 0.6755340461360362 2312 74-75 0.5978120660348267 2312 76-77 0.7293460322564016 2312 78-79 0.7639880411600792 2312 80-81 0.7374518139186783 2312 82-83 0.7666595659255897 2312 84-85 0.7148633308100365 2312 86-87 0.7493327474459939 2312 88-89 0.7665626677716801 2312 90-91 0.820458901308708 2312 92-93 0.8699977496875952 2312 94-95 0.895832172403253 2312 96-97 0.8529138102190892 2312 98-99 0.9334275880427931 2312 100 1.0501238463182823 2313 1 0.5736346183078176 2313 2 0.5026808878407039 2313 3 0.585987459095314 2313 4 0.4838595045506011 2313 5 0.4888713888989926 2313 6 0.501038302031958 2313 7 0.5660985509588556 2313 8 0.5382691369687151 2313 9 0.6418005675093639 2313 10-11 0.6838006781829549 2313 12-13 0.6907369910445098 2313 14-15 0.827142938073699 2313 16-17 0.8084490616993918 2313 18-19 0.7626950284987828 2313 20-21 0.8010327816587974 2313 22-23 0.7332323397705522 2313 24-25 0.7911338308460287 2313 26-27 0.8512192118183961 2313 28-29 0.852758637430405 2313 30-31 0.9123248596150262 2313 32-33 0.9603190579627494 2313 34-35 1.0146489309520348 2313 36-37 0.9691268629797705 2313 38-39 1.0109284768228974 2313 40-41 1.0368633553072328 2313 42-43 0.9012243315916137 2313 44-45 1.0407129232420758 2313 46-47 1.0451649652158466 2313 48-49 1.0576818269659043 2313 50-51 1.075469707058211 2313 52-53 0.8770908875651457 2313 54-55 0.9219836673980595 2313 56-57 0.9808793627791275 2313 58-59 0.8826915379143117 2313 60-61 0.8738578279576643 2313 62-63 0.9000025674650161 2313 64-65 0.9401285457443791 2313 66-67 0.8267264238014249 2313 68-69 0.7885217292800704 2313 70-71 0.9061543141833681 2313 72-73 0.9838030119829142 2313 74-75 0.9167882975464678 2313 76-77 0.9483357693056362 2313 78-79 0.8965336117368174 2313 80-81 0.9237741868557805 2313 82-83 0.8802966763757318 2313 84-85 0.931483152599828 2313 86-87 0.9275836749887034 2313 88-89 0.8970120499916021 2313 90-91 1.0564987506630032 2313 92-93 0.9562696773192947 2313 94-95 1.0225803455395273 2313 96-97 0.991199705941245 2313 98-99 0.9697145227008299 2313 100 1.0391110159003532 2314 1 0.28904230090299876 2314 2 0.2192633288372079 2314 3 0.2594817165739478 2314 4 0.21639868845697663 2314 5 0.22023581440404172 2314 6 0.20092372476894838 2314 7 0.27268302551208023 2314 8 0.267933266242224 2314 9 0.3733993073112245 2314 10-11 0.3440332624389768 2314 12-13 0.4527471922148649 2314 14-15 0.521000230954435 2314 16-17 0.5580997907446488 2314 18-19 0.5784487951314574 2314 20-21 0.5694237981943502 2314 22-23 0.62774871749005 2314 24-25 0.6493849872417101 2314 26-27 0.6634866427381709 2314 28-29 0.6776050583705739 2314 30-31 0.6896094240089639 2314 32-33 0.6664117608328013 2314 34-35 0.7804759208024592 2314 36-37 0.752238857322233 2314 38-39 0.8037068464567341 2314 40-41 0.8201142840659799 2314 42-43 0.7459677392256481 2314 44-45 0.8173893934732419 2314 46-47 0.6130967621515495 2314 48-49 0.6610088168163273 2314 50-51 0.7221718547369207 2314 52-53 0.7083497654060551 2314 54-55 0.7394057852108062 2314 56-57 0.6783507715786712 2314 58-59 0.6167763766866514 2314 60-61 0.5208713829790241 2314 62-63 0.6757255424067452 2314 64-65 0.6936565390184697 2314 66-67 0.654653303779078 2314 68-69 0.6821680785904718 2314 70-71 0.6409310175147596 2314 72-73 0.8020002335981005 2314 74-75 0.7983922729980932 2314 76-77 0.739341985311853 2314 78-79 0.7552106768591891 2314 80-81 0.8443194076939733 2314 82-83 0.843165827623789 2314 84-85 0.8782984358408932 2314 86-87 0.8216013760376555 2314 88-89 0.6411924355782368 2314 90-91 0.7069621436068623 2314 92-93 0.8454194381487454 2314 94-95 0.8947844326850216 2314 96-97 0.8823884108994804 2314 98-99 0.8591655230893345 2314 100 0.7822541358012849 2315 1 0.29461824636100076 2315 2 0.30121730011713765 2315 3 0.335531846039931 2315 4 0.28998651449744983 2315 5 0.2438614261011196 2315 6 0.3112467809975641 2315 7 0.3011990959225699 2315 8 0.29833891642275745 2315 9 0.2713306725240727 2315 10-11 0.3716057307257472 2315 12-13 0.3582580706237408 2315 14-15 0.4831223001534042 2315 16-17 0.279880772013648 2315 18-19 0.47999997428149044 2315 20-21 0.3060589223747954 2315 22-23 0.3226109074843464 2315 24-25 0.5210163105859706 2315 26-27 0.6494066603764281 2315 28-29 0.47538067838197406 2315 30-31 0.5163900266790478 2315 32-33 0.5596757066352467 2315 34-35 0.43677320854951773 2315 36-37 0.5734679007586649 2315 38-39 0.6662545424967377 2315 40-41 0.7239126704922398 2315 42-43 0.7852931798850946 2315 44-45 0.7059507324365626 2315 46-47 0.7211271792339389 2315 48-49 0.5927384496793096 2315 50-51 0.4815815225200808 2315 52-53 0.2961589916389471 2315 54-55 0.4490039358664504 2315 56-57 0.4261194134923869 2315 58-59 0.46414726703252285 2315 60-61 0.5973275041069286 2315 62-63 0.5848929811182657 2315 64-65 0.5624053883747067 2315 66-67 0.38922736515070966 2315 68-69 0.48848303428697903 2315 70-71 0.41666453435721706 2315 72-73 0.46745022439294814 2315 74-75 0.22271075783039151 2315 76-77 0.36716410617843565 2315 78-79 0.475077692133798 2315 80-81 0.5470852954861627 2315 82-83 0.5442656574769806 2315 84-85 0.46352457817033255 2315 86-87 0.4529389734340832 2315 88-89 0.4086439155288275 2315 90-91 0.6053837944169196 2315 92-93 0.7445839223040096 2315 94-95 0.6136098184105059 2315 96-97 0.619891788595698 2315 98-99 0.7500821184903224 2315 100 0.6956662622503522 2316 1 0.003920332080827649 2316 2 0.038125043377238654 2316 3 0.07181482136609674 2316 4 -0.09161634113309702 2316 5 -0.07032332964907084 2316 6 -0.15171547485576298 2316 7 -0.07578394427236645 2316 8 -0.023084764454466722 2316 9 -0.015145065764215815 2316 10-11 0.009829217259991196 2316 12-13 -0.0016886869469914245 2316 14-15 -0.23356563100669092 2316 16-17 -0.10340733312467165 2316 18-19 -0.019291428718624104 2316 20-21 0.017120869860058008 2316 22-23 -0.04512975874166614 2316 24-25 -0.07626282177002253 2316 26-27 -0.19262525523622998 2316 28-29 -0.07503098377723205 2316 30-31 -0.6313450962951208 2316 32-33 -0.5098045443779426 2316 34-35 -0.5896596101347953 2316 36-37 -0.2775574252749564 2316 38-39 -0.25114957855770115 2316 40-41 0.06401509117721815 2316 42-43 -0.03275056572046253 2316 44-45 -0.1553857881568561 2316 46-47 -0.26627596747220394 2316 48-49 -0.15796235692308613 2316 50-51 0.06066766281043812 2316 52-53 -0.29965346123463377 2316 54-55 -0.2740026660016639 2316 56-57 -0.27669908648523034 2316 58-59 -0.6702797722740925 2316 60-61 -0.12890563900904084 2316 62-63 -0.1514221433130416 2316 64-65 -0.4623585296641721 2316 66-67 -0.7993823275960139 2316 68-69 -0.8152567734591756 2316 70-71 -0.541928844638818 2316 72-73 -0.631162821516412 2316 74-75 -0.37674101763411727 2316 76-77 -0.15253638266351288 2316 78-79 -0.14818812149531269 2316 80-81 -0.20197634564994615 2316 82-83 -0.024387714163161434 2316 84-85 -0.13027674518778198 2316 86-87 0.06550714489831932 2316 88-89 0.10140161090541255 2316 90-91 -0.4678735924160371 2316 92-93 -0.5492114979078409 2316 94-95 -0.1466492572928466 2316 96-97 0.13926321543662468 2316 98-99 0.1668890123118132 2316 100 0.0319430491480901 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 109.0 1 281.0 2 168.0 3 177.0 4 175.0 5 147.0 6 193.0 7 690.0 8 1822.0 9 2638.0 10 3830.0 11 6078.0 12 9175.0 13 12068.0 14 14229.0 15 16463.0 16 19645.0 17 23938.0 18 29134.0 19 33858.0 20 39300.0 21 45938.0 22 50380.0 23 51927.0 24 51852.0 25 52849.0 26 56841.0 27 63826.0 28 74318.0 29 87454.0 30 104424.0 31 127194.0 32 158830.0 33 202027.0 34 274206.0 35 383127.0 36 554921.0 37 808338.0 38 765275.0 39 180145.0 40 188.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.80912223382843 18.18205880038012 12.792004608225385 23.216814357566065 2 16.282098234120753 20.27324281165008 40.07641020302313 23.368248751206036 3 19.006615967004205 26.30033769336811 29.773824840544005 24.919221499083683 4 12.748319079773657 16.874205738785392 38.20832313784776 32.169152043593186 5 13.299030917677454 36.5842390282339 34.41846470726712 15.698265346821522 6 29.73891573908851 36.25810343978769 19.380330110380484 14.62265071074331 7 26.8168998384885 32.38520317821186 22.770334367115282 18.02756261618436 8 25.769315167101087 34.20892715124143 21.114727071130435 18.90703061052705 9 26.40224147807154 16.61479134977446 21.17146810358779 35.81149906856621 10-11 24.715786393970212 25.867977671584676 28.600724001653266 20.815511932791843 12-13 25.427815293808276 24.00254024128809 28.452095611988593 22.117548852915036 14-15 22.990007358013443 25.277109659012147 26.77035219784953 24.96253078512488 16-17 22.109459027691347 27.77143353389297 27.434684216099136 22.684423222316546 18-19 21.723291925249505 26.886693987224863 29.562313790751833 21.8277002967738 20-21 22.808711961338485 26.23571694218643 29.422149881989775 21.53342121448532 22-23 22.569378607654507 26.163387118854093 29.164519089124457 22.102715184366943 24-25 22.100716757592533 26.25774588627084 29.496562538868066 22.144974817268565 26-27 22.05110631885426 26.340959385255452 29.28511235514703 22.322821940743257 28-29 21.962366630821712 26.551031567130444 29.382991888961698 22.103609913086146 30-31 22.558886278403925 26.345549923028493 29.313069085786598 21.782494712780988 32-33 22.288806204617796 26.54649271852833 29.09249406863042 22.072207008223454 34-35 22.528255048986253 26.581005547673065 29.042660653784797 21.848078749555885 36-37 22.503585592056048 26.51833409323619 28.870619485116762 22.107460829591 38-39 22.33272205710204 26.755344680607973 28.866314422318418 22.045618839971567 40-41 22.5590337914061 26.351304981940487 29.007136134643087 22.08252509201033 42-43 22.325097194030405 26.51334705120047 28.777549920804862 22.384005833964263 44-45 22.315035591662102 26.804442515741002 28.668593486306044 22.211928406290852 46-47 22.25848635980045 26.536519122013296 28.395156494430918 22.80983802375534 48-49 22.283028898667816 26.59192113769155 28.503650219909694 22.62139974373094 50-51 22.311589975494247 26.962387666491406 28.201702397253275 22.524319960761073 52-53 22.364124236966827 27.122998554876375 27.92082431969795 22.592052888458845 54-55 22.23001357739434 26.97826978269783 28.130426588887808 22.66129005102002 56-57 22.35966265086543 27.00124980680423 27.933931052885885 22.705156489444455 58-59 22.269721173735928 27.003446167308766 28.13217685417573 22.59465580477958 60-61 22.49135506566272 27.08639268002428 28.00929696924237 22.412955285070634 62-63 22.43584190667335 27.020286412532425 28.01281129764901 22.531060383145217 64-65 22.26087822870259 26.772924817492903 28.06823197366143 22.897964980143072 66-67 22.2023224449013 26.86747456614544 27.883981098566018 23.04622189038724 68-69 22.079373173009717 26.921201523683624 27.95700260779221 23.04242269551445 70-71 22.220947436494285 26.68480836428721 27.988546437315165 23.10569776190334 72-73 22.22742727598621 26.93630419325144 27.77527266823817 23.06099586252418 74-75 22.31740265748141 27.00201717033222 27.64545177555578 23.035128396630586 76-77 22.253777399755574 26.942333874240525 27.681412435446738 23.122476290557152 78-79 22.190548133473225 26.922972856062056 27.716905025539074 23.16957398492565 80-81 22.239796320076813 27.094799561220455 27.494908350305497 23.170495768397235 82-83 22.514345006581472 27.03280145879219 27.65599759221259 22.796855942413753 84-85 22.549628000469134 27.123741663831336 27.35670731353284 22.96992302216669 86-87 22.30151886487256 27.207644464716306 27.558975297457238 22.93186137295389 88-89 22.418234042518602 26.882738121090654 27.55146786090793 23.147559975482814 90-91 22.66694198492307 26.92554479974255 27.480764788591234 22.926748426743142 92-93 22.644109654238566 26.99954391838334 27.450526990868262 22.905819436509827 94-95 22.39140569631853 26.956583649071508 27.500103949113946 23.151906705496017 96-97 22.544957469838657 26.923357443823488 27.393384248742215 23.13830083759564 98-99 22.53894047527096 26.91594223435078 27.23650244523129 23.308614845146966 100 22.841668351565097 26.81872584494751 27.03632176214812 23.30328404133927 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2252.0 1 1665.0 2 3193.0 3 4379.5 4 2459.5 5 1594.0 6 2226.0 7 3649.0 8 5570.0 9 6525.0 10 5518.0 11 4410.5 12 4378.0 13 4697.5 14 5086.0 15 5493.5 16 5804.0 17 6291.5 18 6825.0 19 7234.5 20 7842.5 21 8673.0 22 9854.0 23 11571.0 24 13740.5 25 16373.0 26 19951.0 27 24420.5 28 29555.0 29 35844.5 30 43760.0 31 52049.0 32 61320.5 33 71554.5 34 81617.5 35 90766.5 36 101485.5 37 113641.0 38 121265.0 39 125456.5 40 128267.0 41 130571.0 42 134277.0 43 139788.0 44 145690.0 45 152629.5 46 158968.5 47 162974.0 48 166906.5 49 170588.5 50 169633.0 51 165421.5 52 160377.0 53 154529.0 54 148783.0 55 138970.5 56 126235.5 57 112362.5 58 99306.5 59 87178.0 60 71918.5 61 57025.5 62 45889.0 63 36311.5 64 27475.0 65 20442.5 66 15392.0 67 11941.5 68 9277.0 69 7103.0 70 5528.5 71 4308.5 72 3355.0 73 2554.0 74 2009.5 75 1561.0 76 1152.0 77 775.5 78 518.0 79 352.0 80 252.0 81 177.0 82 127.0 83 99.0 84 67.0 85 48.5 86 40.0 87 34.5 88 25.5 89 21.0 90 17.0 91 13.5 92 14.0 93 10.5 94 7.5 95 6.5 96 3.0 97 1.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025463200452720384 2 0.11201951265709077 3 0.016712401391028878 4 0.01661955471663427 5 0.018662181553315577 6 0.04600552716252671 7 0.04602873883112536 8 0.03836888819357046 9 0.031962467660342726 10-11 0.03616377967669859 12-13 0.034829108732276154 14-15 0.030384074195634443 16-17 0.0362566263510932 18-19 0.035537064624535014 20-21 0.046040344665424685 22-23 0.04571538130504357 24-25 0.06377405947479423 26-27 0.055464282116477075 28-29 0.04949888328662372 30-31 0.04732859227264983 32-33 0.04263983521572229 34-35 0.04335939694228048 36-37 0.0396107124635983 38-39 0.04380041864565485 40-41 0.05281815189623084 42-43 0.054733114555619564 44-45 0.05720515726137592 46-47 0.05982807581302351 48-49 0.059120119920764644 50-51 0.052226254346965234 52-53 0.061835885146806845 54-55 0.04928997826923586 56-57 0.05367698363438094 58-59 0.051878079317985465 60-61 0.04743304478134376 62-63 0.05317793275950994 64-65 0.05221464851266591 66-67 0.050032751664392694 68-69 0.056369537191824484 70-71 0.056717712220804246 72-73 0.05880676239468286 74-75 0.04981224081270551 76-77 0.049684576635412925 78-79 0.05850501070290039 80-81 0.060327126687894514 82-83 0.04990508748710012 84-85 0.055545522956572356 86-87 0.052574429375945 88-89 0.060977053408656744 90-91 0.06632734302064584 92-93 0.07027332668241656 94-95 0.07397558782390143 96-97 0.06958858245875635 98-99 0.07823492901175393 100 0.12181483680572158 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4308178.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.38183289338897 #Duplication Level Percentage of deduplicated Percentage of total 1 80.6876358980186 55.1756843454092 2 12.575619231497823 17.19887785638345 3 3.3920910149540227 6.9587220283125655 4 1.2614256120475438 3.4503438164190414 5 0.6106634451552797 2.087914283035477 6 0.36375606809320943 1.4924583997383638 7 0.22265045271068817 1.0657672235629778 8 0.17215213521136488 0.9417662833810924 9 0.11981320056254981 0.7373741633361339 >10 0.5574418156450714 6.612526091035918 >50 0.025583546516104408 1.2054960592218948 >100 0.010197387617996741 1.2177601848357826 >500 6.597305650972724E-4 0.3130882719746605 >1k 1.5523070234046876E-4 0.2752724488351535 >5k 7.76153511702335E-5 0.40615490498621465 >10k+ 7.76153511702335E-5 0.8607936395320382 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18267 0.42400755029156173 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14170 0.32890934404288774 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8320 0.19312108274077813 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6985 0.16213350516157873 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 2.3211668598651217E-5 0.0 0.0 3 0.0 0.0 2.3211668598651217E-5 0.0 0.0 4 0.0 0.0 2.3211668598651217E-5 0.0 0.0 5 0.0 0.0 2.3211668598651217E-5 0.0 0.0 6 0.0 0.0 2.3211668598651217E-5 0.0 0.0 7 0.0 0.0 2.3211668598651217E-5 0.0 0.0 8 0.0 0.0 2.3211668598651217E-5 0.0 0.0 9 0.0 0.0 2.3211668598651217E-5 0.0 6.963500579595365E-5 10-11 0.0 0.0 2.3211668598651217E-5 0.0 8.124084009527926E-5 12-13 0.0 0.0 2.3211668598651217E-5 0.0 1.392700115919073E-4 14-15 0.0 0.0 2.3211668598651217E-5 0.0 1.7408751448988414E-4 16-17 0.0 0.0 2.3211668598651217E-5 2.3211668598651217E-5 2.0890501738786096E-4 18-19 0.0 0.0 2.3211668598651217E-5 2.3211668598651217E-5 2.3211668598651215E-4 20-21 0.0 0.0 2.3211668598651217E-5 2.3211668598651215E-4 2.3211668598651215E-4 22-23 0.0 0.0 2.3211668598651217E-5 8.58831738150095E-4 2.553283545851634E-4 24-25 0.0 0.0 2.3211668598651217E-5 0.0023791960313617497 2.553283545851634E-4 26-27 0.0 0.0 2.3211668598651217E-5 0.003969195330369359 2.553283545851634E-4 28-29 0.0 0.0 2.3211668598651217E-5 0.008019631500833995 2.553283545851634E-4 30-31 0.0 0.0 2.3211668598651217E-5 0.019381743279873766 2.66934188884489E-4 32-33 0.0 0.0 2.3211668598651217E-5 0.047943701490514086 2.785400231838146E-4 34-35 0.0 0.0 2.3211668598651217E-5 0.09052550753473974 3.017516917824658E-4 36-37 0.0 0.0 2.3211668598651217E-5 0.15534409209647326 3.017516917824658E-4 38-39 0.0 0.0 2.3211668598651217E-5 0.24877105820604442 3.017516917824658E-4 40-41 0.0 0.0 2.3211668598651217E-5 0.3583997689974741 3.2496336038111704E-4 42-43 0.0 0.0 2.3211668598651217E-5 0.46674023218167865 3.4817502897976823E-4 44-45 0.0 0.0 2.3211668598651217E-5 0.5980138239413506 3.5978086327909385E-4 46-47 0.0 0.0 2.3211668598651217E-5 0.7554121487088046 3.713866975784195E-4 48-49 0.0 0.0 2.3211668598651217E-5 0.9158395962283824 3.713866975784195E-4 50-51 0.0 0.0 2.3211668598651217E-5 1.0812691583309695 3.713866975784195E-4 52-53 0.0 0.0 2.3211668598651217E-5 1.2466755087649581 3.8299253187774505E-4 54-55 0.0 2.3211668598651217E-5 2.3211668598651217E-5 1.4437309693332077 3.9459836617707067E-4 56-57 0.0 4.6423337197302435E-5 2.3211668598651217E-5 1.6677699946473892 4.062042004763963E-4 58-59 0.0 4.6423337197302435E-5 2.3211668598651217E-5 1.909635581445335 4.178100347757219E-4 60-61 0.0 4.6423337197302435E-5 2.3211668598651217E-5 2.1515708032490766 4.410217033743731E-4 62-63 0.0 4.6423337197302435E-5 2.3211668598651217E-5 2.4054252168782257 4.410217033743731E-4 64-65 0.0 4.6423337197302435E-5 2.3211668598651217E-5 2.6590126963184897 4.410217033743731E-4 66-67 0.0 4.6423337197302435E-5 2.3211668598651217E-5 2.951711837347482 4.410217033743731E-4 68-69 0.0 4.6423337197302435E-5 2.3211668598651217E-5 3.267622646975125 4.410217033743731E-4 70-71 0.0 4.6423337197302435E-5 2.3211668598651217E-5 3.6008377555430626 4.642333719730243E-4 72-73 0.0 4.6423337197302435E-5 2.3211668598651217E-5 3.9281454944526435 6.035033835649316E-4 74-75 0.0 4.6423337197302435E-5 2.3211668598651217E-5 4.267128702667346 6.035033835649316E-4 76-77 0.0 6.963500579595365E-5 2.3211668598651217E-5 4.627791609353189 6.035033835649316E-4 78-79 0.0 6.963500579595365E-5 2.3211668598651217E-5 5.020463407036571 6.035033835649316E-4 80-81 0.0 8.124084009527926E-5 2.3211668598651217E-5 5.4260292866265045 6.151092178642572E-4 82-83 0.0 9.284667439460487E-5 2.3211668598651217E-5 5.83550633237531 6.267150521635828E-4 84-85 0.0 9.284667439460487E-5 2.3211668598651217E-5 6.253803812191604 6.267150521635828E-4 86-87 0.0 9.284667439460487E-5 2.3211668598651217E-5 6.695417413115242 6.267150521635828E-4 88 0.0 1.1605834299325608E-4 2.3211668598651217E-5 7.03912419588977 6.267150521635828E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7815 0.0 49.602352 1 TACCTGG 4385 0.0 41.40917 2 GTATCAA 13795 0.0 41.263657 1 GTACCTG 5225 0.0 35.834457 1 ACCTGGG 5255 0.0 33.713852 3 ATCAACG 16750 0.0 33.330463 3 CAACGCA 16965 0.0 33.157753 5 TATCAAC 17245 0.0 32.624718 2 TCAACGC 17410 0.0 32.28287 4 AACGCAG 18010 0.0 31.62486 6 ACGCAGA 20090 0.0 28.118002 7 CGCAGAG 20650 0.0 27.239157 8 GTACATG 14815 0.0 26.038517 1 ATAACGC 655 0.0 25.111141 3 GTGGTAT 3805 0.0 24.85109 1 GCAGAGT 22015 0.0 24.65489 9 TACATGG 15280 0.0 24.47499 2 ACATGGG 15350 0.0 23.665237 3 TAACGCA 620 0.0 23.496857 4 TGGTATC 4060 0.0 22.477835 2 >>END_MODULE