FastQCFastQC Report
Mon 6 Jun 2016
ERR1211298_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211298_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4308178
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159100.36929764740454085No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133140.3090401557224423No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80220.18620400549838007No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57750.13404738615721076No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78600.052.3586161
TACCTGG46250.043.414742
GTATCAA138450.039.1810381
GTACCTG54050.038.1135941
ACCTGGG55550.035.391233
TAACGCA5250.032.1742824
ATAACGC5450.031.854513
ATCAACG170850.031.1707043
CAACGCA172300.030.8216765
TATCAAC175200.030.6067562
TCAACGC175500.030.3715484
AACGCAG183700.029.2409486
CCTGGGG51750.026.7471394
GTGGTAT37400.026.3486751
TATCACG3750.026.2720072
ACGCAGA206600.025.8408667
CGCAGAG212150.025.1206258
TGGTATC38400.024.9232222
GTACATG144650.023.3898751
GCAGAGT231250.022.7807459
Produced by FastQC (version 0.11.5)