##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211298_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4308178 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12725797309211 33.0 31.0 34.0 30.0 34.0 2 32.25229528584938 34.0 31.0 34.0 30.0 34.0 3 32.31999583118432 34.0 31.0 34.0 30.0 34.0 4 35.95399957940457 37.0 35.0 37.0 35.0 37.0 5 35.8641950262965 37.0 35.0 37.0 35.0 37.0 6 35.88350945573744 37.0 35.0 37.0 35.0 37.0 7 35.80638102696778 37.0 35.0 37.0 35.0 37.0 8 35.78704199315813 37.0 35.0 37.0 33.0 37.0 9 37.413196483525056 39.0 37.0 39.0 34.0 39.0 10-11 37.36353883242521 39.0 37.0 39.0 34.0 39.0 12-13 37.29557402224328 39.0 37.0 39.0 34.0 39.0 14-15 38.53898248865298 40.0 38.0 41.0 34.0 41.0 16-17 38.52262754695836 40.0 38.0 41.0 34.0 41.0 18-19 38.540500415720985 40.0 38.0 41.0 34.0 41.0 20-21 38.50616014472939 40.0 38.0 41.0 34.0 41.0 22-23 38.386686204701846 40.0 38.0 41.0 34.0 41.0 24-25 38.29613446798159 40.0 38.0 41.0 34.0 41.0 26-27 38.19434317709249 40.0 38.0 41.0 33.5 41.0 28-29 38.08068584909908 40.0 37.5 41.0 33.0 41.0 30-31 38.06205651205684 40.0 37.0 41.0 33.0 41.0 32-33 37.96843503216441 40.0 37.0 41.0 33.0 41.0 34-35 37.81178586400098 40.0 37.0 41.0 33.0 41.0 36-37 37.64526570164928 40.0 37.0 41.0 33.0 41.0 38-39 37.520057086777754 40.0 37.0 41.0 32.0 41.0 40-41 37.40618447055809 40.0 36.0 41.0 32.0 41.0 42-43 37.29100631403809 40.0 36.0 41.0 31.5 41.0 44-45 37.13290804140404 40.0 36.0 41.0 31.0 41.0 46-47 36.88987107775027 39.0 36.0 41.0 30.5 41.0 48-49 35.32136798897353 39.0 35.0 41.0 23.5 41.0 50-51 36.06596837456577 39.0 35.0 40.0 28.0 41.0 52-53 36.16327041268954 39.0 35.0 40.0 29.0 41.0 54-55 36.00103709735299 38.5 35.0 40.0 28.0 41.0 56-57 35.79563541710672 38.0 35.0 40.0 28.0 41.0 58-59 35.56863260988752 38.0 34.0 40.0 28.0 41.0 60-61 35.31406385715725 38.0 34.0 40.0 27.5 41.0 62-63 35.003582721048204 37.0 34.0 40.0 26.5 41.0 64-65 34.673460915496065 36.5 34.0 39.5 26.0 41.0 66-67 34.34089376065706 36.0 33.0 39.0 26.0 40.5 68-69 33.98018118100042 36.0 33.0 39.0 26.0 40.0 70-71 33.612710988264645 35.0 32.5 38.0 25.5 40.0 72-73 33.22953566449668 35.0 32.0 37.5 25.0 39.5 74-75 32.861955216335076 35.0 32.0 37.0 25.0 39.0 76-77 31.917211405842565 34.0 30.5 36.0 23.5 38.5 78-79 32.34268024673075 35.0 31.5 36.0 25.0 38.5 80-81 32.2041141289891 35.0 32.0 36.0 24.5 37.0 82-83 31.928913684624916 34.0 31.0 35.0 24.0 37.0 84-85 31.640073715617135 34.0 31.0 35.0 23.5 36.5 86-87 31.37586318392601 34.0 31.0 35.0 23.0 36.0 88-89 31.146933808213124 34.0 31.0 35.0 22.0 36.0 90-91 30.92506182427931 34.0 30.5 35.0 20.0 35.0 92-93 30.735818482894626 34.0 30.0 35.0 20.0 35.0 94-95 30.542975359885318 34.0 30.0 35.0 20.0 35.0 96-97 30.354829930425346 34.0 29.5 35.0 19.0 35.0 98-99 30.131802469628692 34.0 29.0 35.0 19.0 35.0 100 27.7753351881004 31.0 25.0 34.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.31372621454920946 1101 2 -0.2917979931180561 1101 3 -0.2941031426148939 1101 4 -0.20983832870775387 1101 5 -0.2676765253691826 1101 6 -0.2566026623630151 1101 7 -0.3110687057467345 1101 8 -0.29646289291022043 1101 9 -0.4829332159337838 1101 10-11 -0.38192454715937885 1101 12-13 -0.44824813623078086 1101 14-15 -0.589125242961785 1101 16-17 -0.5690772665122807 1101 18-19 -0.6392234041480052 1101 20-21 -0.628679061170125 1101 22-23 -0.7683448361906358 1101 24-25 -0.7055981287382238 1101 26-27 -0.7645946445833403 1101 28-29 -0.699130451621734 1101 30-31 -0.6250112719869421 1101 32-33 -0.5961963140407391 1101 34-35 -0.5860799092083511 1101 36-37 -0.6310894570927061 1101 38-39 -0.6970655157454431 1101 40-41 -0.6030982909330547 1101 42-43 -0.753614281532542 1101 44-45 -0.7104945873137254 1101 46-47 -0.7317604637256494 1101 48-49 0.45127627951126925 1101 50-51 -0.4979669952412138 1101 52-53 -0.7847587969199239 1101 54-55 -0.7757261786323184 1101 56-57 -0.7644890636558515 1101 58-59 -0.939039733710878 1101 60-61 -0.9168782355714242 1101 62-63 -0.8842555187415186 1101 64-65 -0.84641176080968 1101 66-67 -0.7359408853359071 1101 68-69 -0.7649341412747717 1101 70-71 -0.735422603428951 1101 72-73 -1.0081298441337267 1101 74-75 -1.129823492111477 1101 76-77 -0.9377519351720274 1101 78-79 -0.7405146704785821 1101 80-81 -0.6983015094901575 1101 82-83 -0.7757292624479817 1101 84-85 -0.6947132830965685 1101 86-87 -0.6759728369259506 1101 88-89 -0.84573828113286 1101 90-91 -0.7621192939941288 1101 92-93 -0.7248420666617896 1101 94-95 -0.8959626854484348 1101 96-97 -0.8814697505636069 1101 98-99 -1.0649527638253176 1101 100 -0.9678504787078559 1102 1 -0.2158166078461896 1102 2 -0.22196925222809938 1102 3 -0.2512467610518101 1102 4 -0.1613875265130531 1102 5 -0.14600757645272466 1102 6 -0.28315274834472604 1102 7 -0.20875686046519348 1102 8 -0.13414320051219875 1102 9 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0.4782183928155632 2311 10-11 0.42956761739890936 2311 12-13 0.5032583195449689 2311 14-15 0.44292619129085153 2311 16-17 0.4334121433469562 2311 18-19 0.6108386182764391 2311 20-21 0.6773876758851429 2311 22-23 0.6857943006656981 2311 24-25 0.5696844062798405 2311 26-27 0.6542906508785222 2311 28-29 0.7004195122766959 2311 30-31 0.5990500061684045 2311 32-33 0.5836869297586986 2311 34-35 0.717559758921297 2311 36-37 0.7559498714945718 2311 38-39 0.7739522044839688 2311 40-41 0.7280375799880332 2311 42-43 0.723254075958458 2311 44-45 0.7931414845576938 2311 46-47 0.8055698548507095 2311 48-49 -6.183946243743954 2311 50-51 -1.109056714760868 2311 52-53 0.3181600038547856 2311 54-55 0.7648857690061632 2311 56-57 0.8598175997647246 2311 58-59 0.8984281576679933 2311 60-61 0.7837390873438466 2311 62-63 0.6305788147878602 2311 64-65 0.7457370862913208 2311 66-67 0.7924135259699412 2311 68-69 0.8569574407475926 2311 70-71 0.8236378014321275 2311 72-73 0.3151903616023546 2311 74-75 0.7109138960251968 2311 76-77 0.8278619640590783 2311 78-79 0.7989813368061291 2311 80-81 0.7364646827638381 2311 82-83 0.6940412626891117 2311 84-85 0.7315914810827095 2311 86-87 0.8240452891420702 2311 88-89 0.9279721215160244 2311 90-91 0.9739784172104677 2311 92-93 0.9548670684363678 2311 94-95 0.9856360719719603 2311 96-97 1.0202842389665925 2311 98-99 1.0345190726661961 2311 100 1.2836867045173896 2312 1 0.03034530958144188 2312 2 0.1682211278929202 2312 3 0.1446680299403198 2312 4 -0.012543823431826695 2312 5 0.20650713074961402 2312 6 0.10237907963807658 2312 7 0.23177287722732132 2312 8 0.32686648208662916 2312 9 0.2351753255941489 2312 10-11 0.20086657733663316 2312 12-13 0.25915147044104003 2312 14-15 0.3305467459945888 2312 16-17 0.4350986944537212 2312 18-19 0.3373824605758884 2312 20-21 0.3942086904290605 2312 22-23 0.5580169514864011 2312 24-25 0.6405356527991799 2312 26-27 0.5774015600884752 2312 28-29 0.6881850583505695 2312 30-31 0.6650444846427419 2312 32-33 0.6901574966181698 2312 34-35 0.31321820027731206 2312 36-37 0.6810030699010596 2312 38-39 0.7284571267489426 2312 40-41 0.5345713839776778 2312 42-43 0.6122902334917981 2312 44-45 0.6620008214895421 2312 46-47 0.7304079377495825 2312 48-49 -4.749410062577919 2312 50-51 -0.8997133801464372 2312 52-53 0.40893636824170443 2312 54-55 0.7000111742076243 2312 56-57 0.7908248312051782 2312 58-59 0.7709439822641926 2312 60-61 0.8315442924810554 2312 62-63 0.6303583394999279 2312 64-65 0.8022918534372181 2312 66-67 0.8113864240234463 2312 68-69 0.731580983435741 2312 70-71 0.7083937294526379 2312 72-73 0.7896255338797644 2312 74-75 0.537255564927527 2312 76-77 0.7381532306633254 2312 78-79 0.4695238144517049 2312 80-81 0.6145360127898059 2312 82-83 0.5653171362525953 2312 84-85 0.6425497609665456 2312 86-87 0.7733777957586057 2312 88-89 0.7479324442418509 2312 90-91 0.46676475302375664 2312 92-93 0.7127377092483975 2312 94-95 0.8824848025814553 2312 96-97 0.9021952825063018 2312 98-99 0.9924826259944375 2312 100 0.4766771631150952 2313 1 0.050577595394464936 2313 2 0.21870361676067773 2313 3 0.3105748332408851 2313 4 0.08789288182074273 2313 5 0.1751268019188572 2313 6 0.277569092646786 2313 7 0.17832144983821507 2313 8 0.27119623165717144 2313 9 0.4357773675559642 2313 10-11 0.3101722857924898 2313 12-13 0.0727397942984851 2313 14-15 0.11691492613047672 2313 16-17 0.054648997785896825 2313 18-19 0.4275658843287147 2313 20-21 0.36103887786831734 2313 22-23 0.45740116815107257 2313 24-25 0.29367150813050813 2313 26-27 0.41600853856797926 2313 28-29 0.38273126599875695 2313 30-31 0.5440441440956576 2313 32-33 0.48001773867387953 2313 34-35 0.40471322555420386 2313 36-37 -0.31813919482194564 2313 38-39 0.1669617681572717 2313 40-41 0.39496822666913545 2313 42-43 0.5340991846916836 2313 44-45 0.532675197475541 2313 46-47 0.39511516159278415 2313 48-49 -5.175880271626031 2313 50-51 -1.243408715343179 2313 52-53 0.16722851676048123 2313 54-55 0.3812487672724174 2313 56-57 0.42394682410053264 2313 58-59 0.4407144462981165 2313 60-61 0.7839154183649342 2313 62-63 0.8952256330499395 2313 64-65 1.0232247999367345 2313 66-67 1.0257442647977157 2313 68-69 1.0040026127978763 2313 70-71 0.9616475184712954 2313 72-73 0.7559645143830522 2313 74-75 0.7633253717338775 2313 76-77 0.795480071276284 2313 78-79 0.7571355181398971 2313 80-81 -0.18167540877572108 2313 82-83 0.304683058338064 2313 84-85 0.7127677472610188 2313 86-87 0.7918665465419679 2313 88-89 0.8532230852973619 2313 90-91 0.870674582570981 2313 92-93 0.8950719000811702 2313 94-95 0.7784339510093581 2313 96-97 0.7544005607030826 2313 98-99 0.9199619376805508 2313 100 0.9738009867261717 2314 1 -0.06715380514943803 2314 2 0.05082061001048288 2314 3 0.15627841351478367 2314 4 0.03391454678772021 2314 5 0.13695390710533673 2314 6 0.1923805182004017 2314 7 -0.018957074588747957 2314 8 0.12174938943689284 2314 9 0.31168518369815246 2314 10-11 0.20175745843234694 2314 12-13 0.1071687442547784 2314 14-15 0.07716078920837077 2314 16-17 0.2630162490242398 2314 18-19 0.42282602783104295 2314 20-21 0.47916871894737056 2314 22-23 0.2364285968855242 2314 24-25 0.34209163184360136 2314 26-27 0.33798301591355084 2314 28-29 0.5034196704762195 2314 30-31 0.45142481680836255 2314 32-33 0.3915879176268646 2314 34-35 0.21048564324068764 2314 36-37 0.3295903542747922 2314 38-39 0.4252667986302683 2314 40-41 0.21051142912640586 2314 42-43 0.2591103389020546 2314 44-45 0.490396263302074 2314 46-47 0.658184481595697 2314 48-49 -6.36171609378998 2314 50-51 -1.5058459634249601 2314 52-53 -0.806585798264777 2314 54-55 -0.37444746791600636 2314 56-57 -0.0782233147883531 2314 58-59 0.3771457667138165 2314 60-61 0.6404903321664079 2314 62-63 0.46164015385898693 2314 64-65 0.7252330889567347 2314 66-67 0.8162213766615452 2314 68-69 0.767148646451723 2314 70-71 0.4750110212879264 2314 72-73 0.6709968236536454 2314 74-75 0.7191955215493309 2314 76-77 0.6504144056051508 2314 78-79 0.3835097623852377 2314 80-81 0.5422300369070712 2314 82-83 0.5217269194248928 2314 84-85 0.5245742133643603 2314 86-87 0.5848368141216156 2314 88-89 0.5089414771485679 2314 90-91 0.6451214247912596 2314 92-93 0.7658824697198661 2314 94-95 0.789421834653492 2314 96-97 0.7647580417636881 2314 98-99 0.941355409659959 2314 100 -0.36312825157025586 2315 1 0.11402077200753524 2315 2 0.21097945823834152 2315 3 0.23622595900604182 2315 4 0.08303374754313353 2315 5 0.1642452471376501 2315 6 0.24780628608808541 2315 7 0.32756894629209654 2315 8 0.3268047242668075 2315 9 0.49826125387109244 2315 10-11 0.2462338487069573 2315 12-13 0.4516323289777944 2315 14-15 0.2687494719216943 2315 16-17 0.3266137775898983 2315 18-19 0.44072328944735517 2315 20-21 0.3468941634670415 2315 22-23 0.3904947121340072 2315 24-25 0.4448100091834917 2315 26-27 0.4646316657258822 2315 28-29 0.5838877523604893 2315 30-31 0.5818152859146863 2315 32-33 0.6904464047752867 2315 34-35 0.7051676646916576 2315 36-37 0.5149987808473853 2315 38-39 0.5881250942521774 2315 40-41 0.6978388325614091 2315 42-43 0.7241918267512588 2315 44-45 0.7095455366890917 2315 46-47 0.5927430278352475 2315 48-49 -4.743245663107729 2315 50-51 -0.886670559289179 2315 52-53 0.27796818251725597 2315 54-55 0.18399744074515212 2315 56-57 0.5001852632238055 2315 58-59 0.6971810729186814 2315 60-61 0.6555988787028966 2315 62-63 0.4075184365343034 2315 64-65 0.5365535306257811 2315 66-67 -0.21856588786477715 2315 68-69 0.4492632624484898 2315 70-71 0.4117275442354753 2315 72-73 0.5596169939192279 2315 74-75 0.6993200434754101 2315 76-77 0.6469958429745013 2315 78-79 0.6178883627866938 2315 80-81 0.5325958451192534 2315 82-83 0.5233454297940483 2315 84-85 0.45908773602684505 2315 86-87 0.5061869720938681 2315 88-89 0.619860492613217 2315 90-91 0.6574264092315865 2315 92-93 0.657365478794361 2315 94-95 0.4002479820309155 2315 96-97 0.4309191675238573 2315 98-99 0.4545363680645593 2315 100 0.4061383503634204 2316 1 0.2066156809937496 2316 2 0.27098921927722586 2316 3 0.16755195927112965 2316 4 -0.047034290057929695 2316 5 0.13164122667808442 2316 6 0.10501839469193186 2316 7 0.24218355642549483 2316 8 0.24359296502387906 2316 9 0.4090596664733681 2316 10-11 0.36122823523181324 2316 12-13 0.3544176045559766 2316 14-15 0.42636984124359145 2316 16-17 0.40646952137316106 2316 18-19 0.21805628307546243 2316 20-21 0.44607963900607217 2316 22-23 0.5208305857952595 2316 24-25 0.5281669331571095 2316 26-27 0.5507107301408354 2316 28-29 0.5646817891732994 2316 30-31 0.5248544231160963 2316 32-33 0.4691612613046914 2316 34-35 0.48430309296162477 2316 36-37 0.37265307680173265 2316 38-39 0.39905620694916166 2316 40-41 0.04127653088737304 2316 42-43 0.07651683247884478 2316 44-45 0.2778945342075758 2316 46-47 0.37131996141190626 2316 48-49 -4.716096566749947 2316 50-51 -0.9964702868227704 2316 52-53 0.27690495784570146 2316 54-55 0.521012230650193 2316 56-57 0.3352888820938631 2316 58-59 0.23226178143667653 2316 60-61 0.36866570144085387 2316 62-63 0.31025620166018086 2316 64-65 0.33276540047040015 2316 66-67 0.48905632481878314 2316 68-69 0.1471156583358635 2316 70-71 -0.23905779366588575 2316 72-73 -0.02254876730575006 2316 74-75 0.19879325899159994 2316 76-77 0.28548156964960825 2316 78-79 0.27555810786733304 2316 80-81 0.17877354693882097 2316 82-83 0.07784167416411591 2316 84-85 0.21341689282933274 2316 86-87 0.26239076186988086 2316 88-89 0.12839800019514414 2316 90-91 0.06985213711805116 2316 92-93 0.22058049464288487 2316 94-95 0.4408502170630335 2316 96-97 0.4805535455165746 2316 98-99 0.5046722940421766 2316 100 -0.1904480949594003 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 3.0 11 7.0 12 28.0 13 117.0 14 481.0 15 1434.0 16 3400.0 17 7174.0 18 11929.0 19 16627.0 20 21792.0 21 29034.0 22 34999.0 23 37370.0 24 38344.0 25 41342.0 26 47546.0 27 57092.0 28 69516.0 29 85265.0 30 104678.0 31 128469.0 32 160842.0 33 208219.0 34 287033.0 35 407375.0 36 628072.0 37 954441.0 38 813569.0 39 111971.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.70515356681805 19.016466523641025 13.648570207944424 23.6298097015965 2 16.01343979944755 20.68454771244864 39.66590840509737 23.63610408300643 3 18.67999357500715 26.083196229680496 30.399616914443605 24.837193280868746 4 12.753127424708943 17.226896804387426 38.126512287049316 31.893463483854312 5 13.178357191618579 36.768615369902655 34.461892781281605 15.591134657197166 6 29.93809909344465 36.51120567033473 19.066421613230286 14.484273622990331 7 26.94339160159854 32.40512170228846 22.48598421095512 18.165502485157877 8 25.957815940564227 34.55370477750815 20.767886929784694 18.72059235214293 9 26.25409407368253 16.568552824896333 20.96181975234873 36.2155333490724 10-11 24.851530469301828 25.985168018647563 28.394755674338917 20.76854583771169 12-13 25.37662993920047 24.01693571140166 28.43817027374355 22.168264075654317 14-15 22.886600498610605 25.368047674053827 26.67954374611131 25.06580808122425 16-17 22.13415927071342 27.83770223707106 27.355558544718267 22.672579947497255 18-19 21.734329655637445 26.886566303268612 29.52254172442988 21.856562316664068 20-21 22.760367329724225 26.27621964113326 29.346105646175303 21.617307382967212 22-23 22.496227515680093 26.207305238025715 29.14741738311566 22.149049863178533 24-25 22.077719201815338 26.2698761610482 29.461133899822105 22.191270737314355 26-27 22.05093804221045 26.363123596418436 29.268692659884987 22.317245701486126 28-29 21.975888395274705 26.58638904602063 29.350405384420586 22.08731717428408 30-31 22.535777680131417 26.33810136945537 29.359830624575363 21.766290325837854 32-33 22.29134167177373 26.56798428881832 29.093566029676676 22.047108009731275 34-35 22.52382397853912 26.59886044218372 29.072503016630208 21.80481256264695 36-37 22.503154568639584 26.52974232949368 28.862776554386855 22.104326547479886 38-39 22.306846599684558 26.737344853856946 28.88220716163686 22.073601384821636 40-41 22.545237190219332 26.33417576072354 29.07666282690003 22.043924222157095 42-43 22.322469522969993 26.503360351164098 28.825423649142312 22.348746476723598 44-45 22.252770243299956 26.75756324676496 28.770463366420294 22.219203143514786 46-47 22.244560947324963 26.533158962050567 28.40136301173733 22.820917078887142 48-49 22.371310267684045 26.651810997346892 28.43403307334933 22.542845661619733 50-51 22.267349766832638 26.909210871191835 28.23442380022756 22.589015561747967 52-53 22.27996157296401 27.08781014184015 27.956307122838364 22.675921162357476 54-55 22.201694727379405 26.92680497867517 28.194942978070454 22.676557315874966 56-57 22.34578215448956 26.970408215140218 27.92228654147973 22.761523088890492 58-59 22.24106134412982 26.99268690512987 28.14925784396077 22.61699390677954 60-61 22.415198683736495 27.139079079804045 27.984492399172506 22.461229837286954 62-63 22.370083402015776 27.035603098004714 27.99171376941664 22.60259973056287 64-65 22.182388496684563 26.77050004881192 28.014559765814223 23.0325516886893 66-67 22.09117819353889 26.824273196518988 27.93314320462457 23.15140540531755 68-69 21.953680394863873 26.901831060934285 27.93346586479098 23.21102267941086 70-71 22.065280672762533 26.683755057879377 27.97183898138329 23.2791252879748 72-73 22.02503588196262 26.92422973928637 27.783017093790875 23.267717284960128 74-75 22.12325994171446 26.975772548040073 27.58055350370193 23.320414006543544 76-77 21.992121042369913 26.940166328906955 27.656281140820322 23.41143148790281 78-79 21.8870703593353 26.982414518729332 27.68965241079294 23.440862711142422 80-81 21.93250131033641 27.131751366162078 27.418330875146875 23.517416448354638 82-83 22.100876260819774 27.08174189846722 27.59474640484199 23.222635435871013 84-85 22.174933845351067 27.170519665880573 27.310194636002873 23.344351852765495 86-87 21.835884785605455 27.23101792062686 27.49526227961982 23.43783501414786 88-89 21.872475621425234 26.966731084879953 27.488249268068078 23.67254402562673 90-91 22.111320370109826 26.966028535064734 27.429304277287986 23.493346817537454 92-93 21.97172043773346 27.075039607335732 27.396785006773495 23.55645494815731 94-95 21.666308022328337 26.977308375401503 27.486815468086572 23.869568134183588 96-97 21.647633756617456 27.037580252925906 27.457053651439217 23.85773233901742 98-99 21.603399183518068 26.994373773216747 27.315603812415773 24.086623230849412 100 21.637726317431987 26.93643293564299 27.386647106069567 24.039193640855455 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2244.0 1 1643.0 2 2785.5 3 3880.0 4 2353.0 5 1504.5 6 1989.0 7 3320.5 8 5025.5 9 5885.0 10 5100.5 11 4042.5 12 3900.5 13 4298.0 14 4803.0 15 5254.0 16 5613.0 17 5934.5 18 6529.5 19 7190.0 20 7888.0 21 8780.5 22 9899.0 23 11574.0 24 13668.5 25 16403.0 26 20176.5 27 24573.5 28 29596.0 29 36096.5 30 44227.5 31 52305.5 32 61488.5 33 71577.5 34 81315.5 35 90913.0 36 101779.0 37 114155.0 38 121646.0 39 125079.5 40 127766.0 41 130442.5 42 134436.0 43 140078.0 44 146548.0 45 154096.0 46 160492.5 47 164617.0 48 168784.0 49 171595.0 50 170905.0 51 167354.5 52 162302.0 53 156838.0 54 150701.0 55 140576.0 56 127819.5 57 113386.5 58 99408.5 59 86334.5 60 70620.0 61 55670.5 62 44550.5 63 34731.0 64 25760.5 65 18933.5 66 14033.5 67 10721.5 68 8244.0 69 6190.0 70 4721.5 71 3685.5 72 2960.5 73 2325.0 74 1732.0 75 1265.0 76 897.5 77 531.0 78 298.5 79 190.0 80 124.5 81 66.5 82 45.5 83 30.5 84 18.5 85 12.0 86 7.0 87 4.0 88 3.0 89 1.5 90 0.5 91 1.0 92 1.0 93 1.5 94 1.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02920027909710323 2 0.0018105101506947947 3 4.642333719730243E-5 4 0.022283201854705167 5 7.195617265581877E-4 6 4.642333719730243E-5 7 2.3211668598651214E-5 8 0.0046423337197302435 9 0.00991138249162407 10-11 0.001276641772925817 12-13 8.124084009527926E-5 14-15 1.5087584589123292E-4 16-17 5.222625434696524E-4 18-19 1.1605834299325607E-5 20-21 1.1605834299325609E-4 22-23 2.0890501738786096E-4 24-25 4.642333719730243E-5 26-27 6.963500579595365E-5 28-29 0.0010445250869393049 30-31 0.0026693418888448896 32-33 0.009702477474236209 34-35 0.018696999056213554 36-37 0.021656486802541584 38-39 0.02108780092187463 40-41 0.0044218228680430564 42-43 0.005118172926002593 44-45 0.009017733250575997 46-47 0.011872768488210098 48-49 1.2252975619856004 50-51 0.017571233129178968 52-53 0.018430064867329066 54-55 0.009412331616753068 56-57 0.007590215631758948 58-59 0.00829817152401781 60-61 0.005245837103295175 62-63 0.0071607997626839 64-65 0.019230867433982533 66-67 0.029502030788885695 68-69 0.02961808913187895 70-71 0.02693714140873474 72-73 0.023211668598651217 74-75 0.00974890081143351 76-77 0.014588533714252288 78-79 0.008425835701310391 80-81 0.002878246906232751 82-83 0.01424035868527252 84-85 0.024035682833903334 86-87 0.02299115774696403 88-89 0.033076627753077986 90-91 0.021261888436364514 92-93 0.01345116195291838 94-95 0.0067081722250102015 96-97 0.003655837804287567 98-99 0.015621452966892269 100 0.03623341468249455 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4308178.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.99616862618929 #Duplication Level Percentage of deduplicated Percentage of total 1 80.20657298818614 54.53739661833465 2 12.954610135201818 17.617277104794475 3 3.408684491042856 6.953324563392788 4 1.2955954987113492 3.5238212000683484 5 0.6342249150734666 2.1562432136133007 6 0.3748705085333862 1.5293854986732875 7 0.2356235577685664 1.1215049416433867 8 0.16369916894059738 0.8904733036201528 9 0.11988349758404453 0.7336456665499843 >10 0.5678210020668915 6.659471538030244 >50 0.026706765499679028 1.2385754805608673 >100 0.01092262590310668 1.3538341110790015 >500 4.709073019152114E-4 0.2302230225037626 >1k 1.5696909309722556E-4 0.3068839697883033 >5k 7.848454654861278E-5 0.36814869412826895 >10k+ 7.848454654861278E-5 0.779791073219144 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15910 0.36929764740454085 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13314 0.3090401557224423 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8022 0.18620400549838007 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5775 0.13404738615721076 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 2.3211668598651217E-5 0.0 9 0.0 0.0 0.0 2.3211668598651217E-5 2.3211668598651217E-5 10-11 0.0 0.0 0.0 2.3211668598651217E-5 2.3211668598651217E-5 12-13 0.0 0.0 0.0 2.3211668598651217E-5 2.3211668598651217E-5 14-15 0.0 0.0 0.0 2.3211668598651217E-5 4.6423337197302435E-5 16-17 0.0 0.0 0.0 4.6423337197302435E-5 4.6423337197302435E-5 18-19 0.0 2.3211668598651217E-5 0.0 4.6423337197302435E-5 4.6423337197302435E-5 20-21 0.0 2.3211668598651217E-5 0.0 2.0890501738786096E-4 4.6423337197302435E-5 22-23 0.0 2.3211668598651217E-5 0.0 8.240142352521182E-4 4.6423337197302435E-5 24-25 0.0 2.3211668598651217E-5 0.0 0.0023559843627630984 4.6423337197302435E-5 26-27 0.0 2.3211668598651217E-5 0.0 0.003969195330369358 6.963500579595365E-5 28-29 0.0 2.3211668598651217E-5 0.0 0.007949996495038043 6.963500579595365E-5 30-31 0.0 2.3211668598651217E-5 0.0 0.019196049931084556 6.963500579595365E-5 32-33 0.0 2.3211668598651217E-5 0.0 0.047525891455738364 9.284667439460487E-5 34-35 0.0 2.3211668598651217E-5 0.0 0.09011930333426335 9.284667439460487E-5 36-37 0.0 2.3211668598651217E-5 0.0 0.15419511450084003 9.284667439460487E-5 38-39 0.0 2.3211668598651217E-5 0.0 0.24330471025106204 9.284667439460487E-5 40-41 0.0 3.4817502897976824E-5 0.0 0.348024153133877 9.284667439460487E-5 42-43 0.0 4.6423337197302435E-5 0.0 0.4520008226215351 1.0445250869393048E-4 44-45 2.3211668598651217E-5 4.6423337197302435E-5 0.0 0.5775643439059389 1.1605834299325608E-4 46-47 2.3211668598651217E-5 4.6423337197302435E-5 0.0 0.7281036206024913 1.392700115919073E-4 48-49 2.3211668598651217E-5 4.6423337197302435E-5 0.0 0.8835869827105565 1.392700115919073E-4 50-51 3.4817502897976824E-5 4.6423337197302435E-5 0.0 1.048668369784164 1.392700115919073E-4 52-53 4.6423337197302435E-5 4.6423337197302435E-5 0.0 1.214306836904139 1.508758458912329E-4 54-55 4.6423337197302435E-5 4.6423337197302435E-5 0.0 1.4108284290946196 1.7408751448988414E-4 56-57 4.6423337197302435E-5 4.6423337197302435E-5 0.0 1.6342639510252361 1.8569334878920974E-4 58-59 4.6423337197302435E-5 4.6423337197302435E-5 0.0 1.876524136189359 1.8569334878920974E-4 60-61 4.6423337197302435E-5 4.6423337197302435E-5 0.0 2.118540598833196 2.0890501738786096E-4 62-63 4.6423337197302435E-5 4.6423337197302435E-5 0.0 2.3725574941425354 2.3211668598651215E-4 64-65 4.6423337197302435E-5 5.802917149662804E-5 0.0 2.6259940977369087 2.4372252028583778E-4 66-67 4.6423337197302435E-5 6.963500579595365E-5 0.0 2.9185075454171114 2.553283545851634E-4 68-69 4.6423337197302435E-5 6.963500579595365E-5 0.0 3.234023756678577 2.553283545851634E-4 70-71 4.6423337197302435E-5 8.124084009527926E-5 0.0 3.566855872714637 2.553283545851634E-4 72-73 4.6423337197302435E-5 9.284667439460487E-5 0.0 3.8951152900367623 3.2496336038111704E-4 74-75 4.6423337197302435E-5 9.284667439460487E-5 0.0 4.234864483315221 3.2496336038111704E-4 76-77 4.6423337197302435E-5 9.284667439460487E-5 0.0 4.596548703419404 3.2496336038111704E-4 78-79 4.6423337197302435E-5 9.284667439460487E-5 0.0 4.988918749411004 3.2496336038111704E-4 80-81 4.6423337197302435E-5 9.284667439460487E-5 0.0 5.394797986527019 3.3656919468044266E-4 82-83 4.6423337197302435E-5 9.284667439460487E-5 0.0 5.805783790734738 3.4817502897976823E-4 84-85 6.963500579595365E-5 9.284667439460487E-5 0.0 6.225253459815264 3.4817502897976823E-4 86-87 6.963500579595365E-5 9.284667439460487E-5 0.0 6.669745307645135 3.5978086327909385E-4 88 6.963500579595365E-5 9.284667439460487E-5 0.0 7.014635885518193 3.9459836617707067E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7860 0.0 52.358616 1 TACCTGG 4625 0.0 43.41474 2 GTATCAA 13845 0.0 39.181038 1 GTACCTG 5405 0.0 38.113594 1 ACCTGGG 5555 0.0 35.39123 3 TAACGCA 525 0.0 32.174282 4 ATAACGC 545 0.0 31.85451 3 ATCAACG 17085 0.0 31.170704 3 CAACGCA 17230 0.0 30.821676 5 TATCAAC 17520 0.0 30.606756 2 TCAACGC 17550 0.0 30.371548 4 AACGCAG 18370 0.0 29.240948 6 CCTGGGG 5175 0.0 26.747139 4 GTGGTAT 3740 0.0 26.348675 1 TATCACG 375 0.0 26.272007 2 ACGCAGA 20660 0.0 25.840866 7 CGCAGAG 21215 0.0 25.120625 8 TGGTATC 3840 0.0 24.923222 2 GTACATG 14465 0.0 23.389875 1 GCAGAGT 23125 0.0 22.780745 9 >>END_MODULE