FastQCFastQC Report
Mon 6 Jun 2016
ERR1211297_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211297_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6575867
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286770.43609458646289534No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239440.3641192864758366No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143060.21755306182439518No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126350.054.1301881
GTATCAA228350.041.1159131
CAACGCA281450.032.7224435
ATCAACG283750.032.5402533
TATCAAC288900.032.1884162
TCAACGC296650.031.2514654
AACGCAG299300.031.0225686
GTACATG303900.028.835841
TACCTGG44200.028.307392
GTGGTAT54550.027.9383451
TACATGG312750.027.4025862
ACGCAGA336750.027.2797247
CGCAGAG345600.026.5529548
ACATGGG314400.026.4237213
GTACTAG23600.025.1135981
CATGGGG219500.025.0888484
TGGTATC60650.024.5849272
ATGGGGG158750.024.3007895
GCAGAGT367150.024.292019
CTGTGCG33850.022.7664819
Produced by FastQC (version 0.11.5)