FastQCFastQC Report
Mon 6 Jun 2016
ERR1211297_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211297_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6575867
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246210.37441450686274524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217710.3310742142442966No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137010.20835275409311046No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA126050.055.0202751
GTATCAA225900.039.466491
ATCAACG281700.030.8619653
CAACGCA281200.030.8611535
TATCAAC289200.030.4796962
TCAACGC293050.029.7307034
AACGCAG299300.029.292186
TACCTGG42700.027.1363352
GTACATG298300.026.6786421
TACATGG305050.025.9126012
ACGCAGA337500.025.587387
ACATGGG305950.025.072773
CGCAGAG347700.024.8367588
GTGGTAT55500.024.180441
CATGGGG210100.023.1796194
TGGTATC59250.022.4859962
GCAGAGT377250.022.4436639
ATGGGGG149800.022.1079565
GAGTACT217050.021.57634712-13
TGGGGGG130050.021.3170076
Produced by FastQC (version 0.11.5)