FastQCFastQC Report
Mon 6 Jun 2016
ERR1211296_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211296_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11251988
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT367340.3264667541415793No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT278840.24781398629291107No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164170.1459031061888797No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA174250.049.42451
GTATCAA325650.043.2008251
CAACGCA399650.034.1919565
ATCAACG401400.034.0433433
TATCAAC415000.033.5706562
TCAACGC422550.032.505744
AACGCAG428200.032.220366
ACGCAGA482100.028.2880177
CGCAGAG494700.027.4705738
GTACATG458050.026.5752941
GTACTAG43500.025.5181671
TACATGG473500.025.3602582
GCAGAGT543250.024.5329979
ACATGGG478450.024.1707553
CATGGGG320250.022.0242394
GTGGTAT86550.021.7923471
GAGTACT302900.021.0146412-13
AGAGTAC511050.020.1758510-11
CAGAGTA533600.020.16871610-11
TACCTGG78000.019.5979942
Produced by FastQC (version 0.11.5)