FastQCFastQC Report
Mon 6 Jun 2016
ERR1211296_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211296_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11251988
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT323260.28729145463006184No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262350.23315879824969596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155620.13830444895604224No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA174850.050.0553361
GTATCAA312700.041.2106821
ATCAACG390850.032.281963
CAACGCA393200.032.2013445
TATCAAC408000.031.3017462
TCAACGC414050.030.6664774
AACGCAG423100.030.4796896
ACGCAGA480300.026.4102767
CGCAGAG494400.025.5811618
GTACATG450750.024.934861
TACATGG465600.024.013292
ACATGGG465150.023.2013933
GCAGAGT549150.022.7479179
GTACTAG42000.022.6821331
GTGGTAT88050.022.3849981
CATGGGG318350.021.2102034
GAGTACT298750.020.41766212-13
TGGTATC93150.020.0466042
CTAGTAC58700.019.983943
AGAGTAC512900.019.82181710-11
Produced by FastQC (version 0.11.5)