##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211296_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11251988 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11327633836794 33.0 31.0 34.0 30.0 34.0 2 32.24620307095955 34.0 31.0 34.0 30.0 34.0 3 32.31141252550216 34.0 31.0 34.0 30.0 34.0 4 35.94811494644324 37.0 35.0 37.0 35.0 37.0 5 35.85280938799437 37.0 35.0 37.0 35.0 37.0 6 35.868263990327755 37.0 35.0 37.0 35.0 37.0 7 35.78550723658788 37.0 35.0 37.0 33.0 37.0 8 35.76953272612804 37.0 35.0 37.0 33.0 37.0 9 37.387701177782986 39.0 37.0 39.0 34.0 39.0 10-11 37.339041109890985 39.0 37.0 39.0 34.0 39.0 12-13 37.26874846471574 39.0 37.0 39.0 34.0 39.0 14-15 38.506827371305405 40.0 38.0 41.0 34.0 41.0 16-17 38.48965778314019 40.0 38.0 41.0 34.0 41.0 18-19 38.49678119102153 40.0 38.0 41.0 34.0 41.0 20-21 38.46815411641036 40.0 38.0 41.0 34.0 41.0 22-23 38.35938484825971 40.0 38.0 41.0 34.0 41.0 24-25 38.26893349868486 40.0 38.0 41.0 34.0 41.0 26-27 38.169201433560005 40.0 38.0 41.0 33.0 41.0 28-29 38.06953277945195 40.0 37.5 41.0 33.0 41.0 30-31 38.078197292780615 40.0 37.0 41.0 33.0 41.0 32-33 38.01866607927417 40.0 37.0 41.0 33.0 41.0 34-35 37.890945271182304 40.0 37.0 41.0 33.0 41.0 36-37 37.747150592410875 40.0 37.0 41.0 33.0 41.0 38-39 37.63448739013941 40.0 37.0 41.0 33.0 41.0 40-41 37.52726660391035 40.0 36.5 41.0 32.0 41.0 42-43 37.42511314445056 40.0 36.0 41.0 32.0 41.0 44-45 37.268134528760605 40.0 36.0 41.0 31.0 41.0 46-47 37.03195888584311 39.0 36.0 41.0 31.0 41.0 48-49 35.476515261125414 39.0 35.0 41.0 24.0 41.0 50-51 36.21594823954665 39.0 35.0 40.0 29.0 41.0 52-53 36.31247847047118 39.0 35.0 40.0 30.0 41.0 54-55 36.14777610854188 38.5 35.0 40.0 29.0 41.0 56-57 35.93181036097799 38.0 35.0 40.0 28.5 41.0 58-59 35.694410223331204 38.0 34.0 40.0 28.0 41.0 60-61 35.44089266714469 37.5 34.0 40.0 28.0 41.0 62-63 35.1269925367855 37.0 34.0 40.0 28.0 41.0 64-65 34.794633934909996 36.5 34.0 39.5 27.0 41.0 66-67 34.45925693308595 36.0 33.0 39.0 26.0 40.5 68-69 34.09888572579352 35.5 33.0 39.0 26.0 40.0 70-71 33.74233406576687 35.0 33.0 38.0 26.0 40.0 72-73 33.36920058037744 35.0 32.0 37.5 26.0 39.5 74-75 33.01088460990182 35.0 32.0 37.0 25.5 39.0 76-77 32.06753913175165 34.0 30.5 36.0 24.5 38.5 78-79 32.50437807079069 35.0 32.0 36.0 25.0 38.0 80-81 32.374189076632504 35.0 32.0 36.0 25.0 37.0 82-83 32.10446367344153 34.0 31.5 35.0 25.0 37.0 84-85 31.822001098828046 34.0 31.0 35.0 24.5 36.5 86-87 31.56008151626184 34.0 31.0 35.0 24.0 36.0 88-89 31.329105710030973 34.0 31.0 35.0 23.5 36.0 90-91 31.109653067529045 34.0 31.0 35.0 23.0 35.0 92-93 30.914645971894032 34.0 30.0 35.0 22.0 35.0 94-95 30.719199176181135 34.0 30.0 35.0 20.0 35.0 96-97 30.52971106083654 34.0 30.0 35.0 20.0 35.0 98-99 30.30920122737422 34.0 29.0 35.0 19.5 35.0 100 27.866564290683566 31.0 25.0 34.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.31559999741068623 1101 2 -0.28482735754511523 1101 3 -0.3203026025436415 1101 4 -0.17738062086282724 1101 5 -0.19029471718555158 1101 6 -0.30459868669939283 1101 7 -0.2629403594495585 1101 8 -0.228197267511959 1101 9 -0.3706902716971001 1101 10-11 -0.3135329895476602 1101 12-13 -0.4363799126222503 1101 14-15 -0.5260876825666188 1101 16-17 -0.6012236839664808 1101 18-19 -0.5155531879568258 1101 20-21 -0.5417198771063241 1101 22-23 -0.6750424019516501 1101 24-25 -0.596266560298659 1101 26-27 -0.6275492964530898 1101 28-29 -0.6545604450629199 1101 30-31 -0.6159816753054983 1101 32-33 -0.5485182132519029 1101 34-35 -0.5919861569865716 1101 36-37 -0.6208198389812125 1101 38-39 -0.6378468535356845 1101 40-41 -0.5808054421097637 1101 42-43 -0.6027745393804764 1101 44-45 -0.6247245721635508 1101 46-47 -0.6538631171922304 1101 48-49 0.526470855406707 1101 50-51 -0.33806589179371116 1101 52-53 -0.6928828874074853 1101 54-55 -0.6909219846257599 1101 56-57 -0.7189042233380221 1101 58-59 -0.8613494482431392 1101 60-61 -0.831684527683187 1101 62-63 -0.8638551225695679 1101 64-65 -0.8192840511200288 1101 66-67 -0.7560111905010274 1101 68-69 -0.7732845038690286 1101 70-71 -0.7895595611683675 1101 72-73 -0.8811424514826669 1101 74-75 -0.8848259565758383 1101 76-77 -0.8623948438904598 1101 78-79 -0.7466140372150605 1101 80-81 -0.5940224400516776 1101 82-83 -0.6233576388352731 1101 84-85 -0.6853296606205781 1101 86-87 -0.6104931545119214 1101 88-89 -0.7000386827040188 1101 90-91 -0.7701330810586811 1101 92-93 -0.7732279856822011 1101 94-95 -0.7752061010806628 1101 96-97 -0.8404555407858574 1101 98-99 -1.0510274825176502 1101 100 -0.8857660811092671 1102 1 -0.25075263215195776 1102 2 -0.2181296893116098 1102 3 -0.2633350949966413 1102 4 -0.1572449410325234 1102 5 -0.1774513315874131 1102 6 -0.21302295030407237 1102 7 -0.21378338178609368 1102 8 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68-69 -0.7214579311483007 1105 70-71 -0.7703807030074827 1105 72-73 -0.7070005441800404 1105 74-75 -0.8275693569872331 1105 76-77 -0.7769116854123368 1105 78-79 -0.6504096711802063 1105 80-81 -0.6272379912899453 1105 82-83 -0.5719077039798215 1105 84-85 -0.5771133663439905 1105 86-87 -0.5767478412382268 1105 88-89 -0.5990027513137477 1105 90-91 -0.6854087346750077 1105 92-93 -0.655865278279812 1105 94-95 -0.6242090796444444 1105 96-97 -0.6708092386787179 1105 98-99 -0.6821422070505214 1105 100 -0.8212012577668695 1106 1 -0.32072824439946146 1106 2 -0.29121771112406236 1106 3 -0.3690631720474009 1106 4 -0.2288678315361352 1106 5 -0.29901072357314007 1106 6 -0.3360242448291544 1106 7 -0.35721592602879326 1106 8 -0.39644474994078394 1106 9 -0.4578452338163146 1106 10-11 -0.476473104738524 1106 12-13 -0.4722406853897141 1106 14-15 -0.668124229025203 1106 16-17 -0.6800250587781633 1106 18-19 -0.6461912097657176 1106 20-21 -0.6414838455461762 1106 22-23 -0.6272001000701053 1106 24-25 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0.2535873975590448 2311 5 0.3126854765401532 2311 6 0.3504967558016361 2311 7 0.3497238541219474 2311 8 0.14033353600103027 2311 9 0.43684712716706287 2311 10-11 0.3403327616742686 2311 12-13 0.44876475607578215 2311 14-15 0.4158037687464997 2311 16-17 0.44838792404369343 2311 18-19 0.5754736738491033 2311 20-21 0.6134814084795792 2311 22-23 0.6337706746423848 2311 24-25 0.6013162711567759 2311 26-27 0.6574567241227456 2311 28-29 0.6914888764876466 2311 30-31 0.5403626366097072 2311 32-33 0.5828738915553728 2311 34-35 0.7049909060586543 2311 36-37 0.6876389002405929 2311 38-39 0.7396094445654526 2311 40-41 0.682971362375028 2311 42-43 0.7323996895534393 2311 44-45 0.7483156759806704 2311 46-47 0.7766348868441497 2311 48-49 -6.071738273278168 2311 50-51 -1.0889888908862702 2311 52-53 0.3518825843792257 2311 54-55 0.7727652118395767 2311 56-57 0.8616251687021403 2311 58-59 0.9134606368777654 2311 60-61 0.7647766498473558 2311 62-63 0.5773294707087899 2311 64-65 0.659673948669365 2311 66-67 0.7664753229573051 2311 68-69 0.8280954624741312 2311 70-71 0.8356193728856098 2311 72-73 0.32293836340924287 2311 74-75 0.6337761709586331 2311 76-77 0.7078313162421672 2311 78-79 0.6664447467388896 2311 80-81 0.6932303685577708 2311 82-83 0.7479992382338594 2311 84-85 0.751908099313848 2311 86-87 0.8042996946155192 2311 88-89 0.839810598954756 2311 90-91 0.9047688016117625 2311 92-93 0.8650687453746606 2311 94-95 0.8974897352451059 2311 96-97 0.9372686019301746 2311 98-99 0.9754580196494942 2311 100 1.228733246186838 2312 1 0.08376503572178251 2312 2 0.25138376844282817 2312 3 0.24149398179161352 2312 4 0.039795637649007176 2312 5 0.21994688464481982 2312 6 0.12623545816908432 2312 7 0.28304805826549995 2312 8 0.35950902742725077 2312 9 0.29747826195657723 2312 10-11 0.29008670101629974 2312 12-13 0.30733232598210236 2312 14-15 0.32943988637863697 2312 16-17 0.45015088730732344 2312 18-19 0.31775186984983605 2312 20-21 0.34134291881927936 2312 22-23 0.5968468748297511 2312 24-25 0.7129586421352343 2312 26-27 0.6554922570406845 2312 28-29 0.7535090457523381 2312 30-31 0.7671247412239808 2312 32-33 0.7816026741808955 2312 34-35 0.45460105694454 2312 36-37 0.7396201760344638 2312 38-39 0.8296436998428831 2312 40-41 0.633740162075398 2312 42-43 0.6446119918354825 2312 44-45 0.7684980806122255 2312 46-47 0.7692574718649325 2312 48-49 -4.873182186970748 2312 50-51 -0.8570613040954029 2312 52-53 0.5227162652611099 2312 54-55 0.8088420897280386 2312 56-57 0.8982805941246141 2312 58-59 0.900235652456125 2312 60-61 0.9238541013582022 2312 62-63 0.6682148854007508 2312 64-65 0.9355549415029429 2312 66-67 0.9064923197542072 2312 68-69 0.8037340745756083 2312 70-71 0.8509374173536841 2312 72-73 0.9635392231886115 2312 74-75 0.5725497133771782 2312 76-77 0.8099765420253142 2312 78-79 0.5075411438461543 2312 80-81 0.6801789725574565 2312 82-83 0.6600336030912999 2312 84-85 0.755927433272543 2312 86-87 0.8665839821945163 2312 88-89 0.8595805916945238 2312 90-91 0.7118996504078474 2312 92-93 0.9125567201947469 2312 94-95 1.011674804886404 2312 96-97 1.0403627839451062 2312 98-99 1.0626546618026502 2312 100 0.6577253598455499 2313 1 0.07782479316483659 2313 2 0.209818294558481 2313 3 0.3055945393857442 2313 4 0.06915210815724748 2313 5 0.1259831264968554 2313 6 0.25854330400864 2313 7 0.1673309975752133 2313 8 0.244657139337761 2313 9 0.46049483186683204 2313 10-11 0.2283843321443655 2313 12-13 0.08733691882027728 2313 14-15 0.19078855912086823 2313 16-17 0.1584540259474423 2313 18-19 0.4325570598182651 2313 20-21 0.4065002636360404 2313 22-23 0.4479504521095308 2313 24-25 0.27638116211979025 2313 26-27 0.41186632072578533 2313 28-29 0.39499045400543054 2313 30-31 0.5819823383638223 2313 32-33 0.5415510594624422 2313 34-35 0.43567024616420724 2313 36-37 -0.3121181784221747 2313 38-39 0.16008825730260412 2313 40-41 0.3844975351147326 2313 42-43 0.44540558588800394 2313 44-45 0.5240068635364352 2313 46-47 0.4239383685784617 2313 48-49 -5.454984442051046 2313 50-51 -1.3562188631412653 2313 52-53 0.12312184038042773 2313 54-55 0.32348748937995 2313 56-57 0.3314790896753763 2313 58-59 0.45518002535867197 2313 60-61 0.7461965393199108 2313 62-63 0.7938905434882244 2313 64-65 0.7893065573246361 2313 66-67 0.9044959998653894 2313 68-69 0.9569678432965887 2313 70-71 0.9461369045883075 2313 72-73 0.7151915669349549 2313 74-75 0.8159176427985315 2313 76-77 0.8377402109195806 2313 78-79 0.7963355651070785 2313 80-81 -0.11938840148344099 2313 82-83 0.29293468001252165 2313 84-85 0.6350561835165358 2313 86-87 0.8095741570756907 2313 88-89 0.7342236453539783 2313 90-91 0.7804885274688296 2313 92-93 0.9009999131788184 2313 94-95 0.8120731819513871 2313 96-97 0.7940400018453708 2313 98-99 0.9714269664873321 2313 100 1.116077215595169 2314 1 0.09226563182101444 2314 2 0.21959505695953396 2314 3 0.2215083610116224 2314 4 0.09236974051363944 2314 5 0.22032871975185486 2314 6 0.31802324712629115 2314 7 0.09740349949017002 2314 8 0.2447990726055167 2314 9 0.4254602283428994 2314 10-11 0.35605541151046083 2314 12-13 0.2793320826676151 2314 14-15 0.29457442285037416 2314 16-17 0.4847140322341872 2314 18-19 0.5441111645694292 2314 20-21 0.6100383670245435 2314 22-23 0.33438931846957587 2314 24-25 0.48327044178240897 2314 26-27 0.5028040748266207 2314 28-29 0.6819426318804958 2314 30-31 0.578937053645447 2314 32-33 0.5672066191348577 2314 34-35 0.4402419950529577 2314 36-37 0.5361108322339305 2314 38-39 0.5717180528948873 2314 40-41 0.32048693404890827 2314 42-43 0.3592256588823517 2314 44-45 0.5375001518320985 2314 46-47 0.6579381015520482 2314 48-49 -6.454678403834386 2314 50-51 -1.5755046057978532 2314 52-53 -0.7753484067936185 2314 54-55 -0.37157478578237857 2314 56-57 -0.012466654133113764 2314 58-59 0.45189619233085665 2314 60-61 0.7061506096040375 2314 62-63 0.5681113279502554 2314 64-65 0.745172764877033 2314 66-67 0.8011729117467041 2314 68-69 0.7922353926825778 2314 70-71 0.5647248673427825 2314 72-73 0.7246744035863912 2314 74-75 0.7747059657729451 2314 76-77 0.6831763230564469 2314 78-79 0.5198944377531873 2314 80-81 0.5487055779406163 2314 82-83 0.530824205823933 2314 84-85 0.5690209197423748 2314 86-87 0.6130408124373865 2314 88-89 0.575159510117004 2314 90-91 0.7865456150789214 2314 92-93 0.8172911106211096 2314 94-95 0.8923873103298057 2314 96-97 0.8683265300869003 2314 98-99 0.9552022133461406 2314 100 -0.4351396094014568 2315 1 0.12140694609328762 2315 2 0.22685315799024863 2315 3 0.30706726381773564 2315 4 0.12121880998744672 2315 5 0.23841195286198058 2315 6 0.3316850917167429 2315 7 0.381754223881984 2315 8 0.2693712605124716 2315 9 0.466348094560189 2315 10-11 0.25492774215905456 2315 12-13 0.4439002018149196 2315 14-15 0.28432867475947177 2315 16-17 0.39375347263761995 2315 18-19 0.5192293209415197 2315 20-21 0.3472387406046451 2315 22-23 0.48780646116299664 2315 24-25 0.4995357164550782 2315 26-27 0.5939459519010555 2315 28-29 0.6284581299149892 2315 30-31 0.6133489912052639 2315 32-33 0.6951460464015966 2315 34-35 0.6917470241399428 2315 36-37 0.5875931208498102 2315 38-39 0.6555410288918182 2315 40-41 0.6836914471833921 2315 42-43 0.7722732782653594 2315 44-45 0.7205549155339881 2315 46-47 0.6386596945137342 2315 48-49 -4.856015307401165 2315 50-51 -0.8726886530553699 2315 52-53 0.30667416699304795 2315 54-55 0.2785017932156393 2315 56-57 0.57940464685786 2315 58-59 0.7487191517003637 2315 60-61 0.6232652535226677 2315 62-63 0.37193273454241904 2315 64-65 0.5307912813597682 2315 66-67 -0.1877194553379269 2315 68-69 0.48773941897336925 2315 70-71 0.4633394758811349 2315 72-73 0.6434682257044599 2315 74-75 0.802296152328573 2315 76-77 0.7664773189703347 2315 78-79 0.7193147506191764 2315 80-81 0.5651605692728765 2315 82-83 0.5469366512880782 2315 84-85 0.575480634104327 2315 86-87 0.6442278944320812 2315 88-89 0.60997624094054 2315 90-91 0.6815111438456469 2315 92-93 0.7288176205298704 2315 94-95 0.35860999533896276 2315 96-97 0.46869658925827906 2315 98-99 0.4132949178157581 2315 100 0.4565290040633343 2316 1 0.24397389842852135 2316 2 0.2537410697183802 2316 3 0.19581173632716542 2316 4 -0.02040232692779398 2316 5 0.15523549857503838 2316 6 0.13057459552310036 2316 7 0.22449046896528557 2316 8 0.2750917227020153 2316 9 0.42231702561065276 2316 10-11 0.39441864843825414 2316 12-13 0.2538361829027522 2316 14-15 0.418546034861059 2316 16-17 0.41573784126095603 2316 18-19 0.15372039864270448 2316 20-21 0.438191530136919 2316 22-23 0.544695972568185 2316 24-25 0.5778391350354468 2316 26-27 0.5901873328465612 2316 28-29 0.632119533255981 2316 30-31 0.5570459766854228 2316 32-33 0.5652496748329625 2316 34-35 0.5404681241941276 2316 36-37 0.39636189695138313 2316 38-39 0.5181102265805322 2316 40-41 0.17837490261001676 2316 42-43 0.0658677479039369 2316 44-45 0.298221063481499 2316 46-47 0.3516728084696581 2316 48-49 -4.937684181221684 2316 50-51 -0.9863499052042926 2316 52-53 0.2770255907017045 2316 54-55 0.547419411797371 2316 56-57 0.44925605688749926 2316 58-59 0.3003428754681394 2316 60-61 0.45316177303313054 2316 62-63 0.35885973160495865 2316 64-65 0.4063350390277378 2316 66-67 0.5212145493703702 2316 68-69 0.1862185371916567 2316 70-71 -0.16854971414284847 2316 72-73 0.06066346405326328 2316 74-75 0.19129379309351435 2316 76-77 0.29632485740716064 2316 78-79 0.37745365430006217 2316 80-81 0.251845221369571 2316 82-83 0.20467014952873797 2316 84-85 0.3372535898203317 2316 86-87 0.3690597588244735 2316 88-89 0.21938326330651137 2316 90-91 0.28879831391898136 2316 92-93 0.36534547894559566 2316 94-95 0.6232785480920882 2316 96-97 0.6716110567239078 2316 98-99 0.6209708117674246 2316 100 -0.25878243382711474 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 4.0 10 15.0 11 12.0 12 59.0 13 211.0 14 900.0 15 2658.0 16 6142.0 17 12836.0 18 21743.0 19 32652.0 20 46510.0 21 64932.0 22 82108.0 23 88343.0 24 91492.0 25 100787.0 26 119261.0 27 146711.0 28 180545.0 29 223157.0 30 273911.0 31 341321.0 32 429013.0 33 558494.0 34 773332.0 35 1094754.0 36 1706978.0 37 2529292.0 38 2053793.0 39 269997.0 40 25.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.06869317821674 19.97994071647842 14.17173623482341 23.77962987048143 2 15.385307241329235 20.30587354491834 40.484780444399256 23.82403876935317 3 18.73588747502327 25.634135453400038 29.745858792942357 25.884118278634332 4 12.822769358338837 16.820524253637807 38.07440136171254 32.28230502631082 5 13.479423956089272 37.71863824129571 34.554875967834825 14.247061834780197 6 31.195550723223437 37.02568595446173 17.721333816092553 14.05742950622228 7 27.7738298334481 32.168342163180405 22.021850716513384 18.03597728685811 8 26.029908821289194 35.70701051323687 19.815669513390624 18.44741115208332 9 26.31448898772582 16.067463317232335 20.59687908163424 37.0211686134076 10-11 25.221569287125135 26.312772891441817 27.727056944412166 20.738600877020886 12-13 25.540900645522285 23.761857252780715 28.194097320167998 22.503144781529 14-15 22.944406152396287 25.272677721823644 25.83559230742945 25.947323818350622 16-17 22.335254038301823 28.188668714608895 26.627303101819283 22.848774145269996 18-19 22.06032189255149 27.379668004341813 28.744960500078896 21.815049603027802 20-21 22.96459102253764 26.520135083816825 28.619659460732986 21.895614432912545 22-23 22.92856292846428 26.351402453563864 28.34227355432063 22.377761063651228 24-25 22.57206129799521 26.456860981748726 28.659170565757165 22.3119071544989 26-27 22.50163871151394 26.67711253143391 28.394463534663966 22.426785222388183 28-29 22.265718178906344 26.79768689239021 28.57196435509954 22.364630573603904 30-31 22.78023017229883 26.532093834874665 28.506897379031965 22.180778613794544 32-33 22.718764712726255 26.72788790290261 28.209735248476807 22.34361213589433 34-35 22.81339259452052 26.753646423479623 28.41342722606568 22.019533755934184 36-37 22.69856969973668 26.624028425731005 28.276618054840714 22.400783819691604 38-39 22.656120169127618 26.66938320492223 28.45358704165028 22.220909584299868 40-41 22.660298605625716 26.326813069856474 28.632304467085945 22.38058385743187 42-43 22.497093140166307 26.45495972613192 28.326464189686572 22.7214829440152 44-45 22.44743958692229 26.8099125425586 28.25497170996461 22.487676160554507 46-47 22.48362346754645 26.634765628385225 27.97147785503323 22.910133049035093 48-49 22.813401626548295 26.70634338900498 27.85312025732712 22.6271347271196 50-51 22.67097251958752 26.75625870086543 27.722466709543863 22.850302070003192 52-53 22.45420995519719 26.967789133235303 27.660389325104482 22.91761158646302 54-55 22.43978730031629 26.877637597712855 27.685831689796174 22.996743412174673 56-57 22.696865824376328 26.804821053854383 27.426244267398186 23.072068854371107 58-59 22.367823098179844 26.80798913739547 27.814119483850302 23.010068280574384 60-61 22.54850280987005 27.07311350310761 27.54140592156894 22.836977765453398 62-63 22.50060482856732 26.868102576791898 27.52193958966937 23.109353004971414 64-65 22.21157460328212 26.720886410498913 27.629735577413474 23.4378034088055 66-67 22.370467784282393 26.692971089892914 27.498176785833113 23.438384339991583 68-69 22.197143128794877 26.782157425419058 27.561625368839216 23.45907407694685 70-71 22.270230151217643 26.521470622329975 27.626946936923545 23.581352289528837 72-73 22.34241675701258 26.80293876378346 27.422818692910067 23.4318257862939 74-75 22.393760073636763 26.92221476998022 27.28004385781691 23.403981298566105 76-77 22.2906246966754 26.823716365857553 27.242904293245246 23.642754644221803 78-79 22.19163095438712 26.71694916519658 27.342345128873514 23.749074751542793 80-81 22.056772265921154 27.069875427864133 27.225794456264662 23.647557849950054 82-83 22.412377209430282 26.7574712086863 27.485661563568435 23.34449001831498 84-85 22.447513187679817 26.84866755997221 27.0912085942234 23.612610658124574 86-87 22.00866763309524 27.063949851202153 27.328890250946223 23.598492264756388 88-89 22.168188744699354 26.66958178070617 27.315578697477438 23.84665077711704 90-91 22.24620720859264 26.636982727431924 27.32263638893013 23.794173675045315 92-93 22.24006147297369 26.7450189314271 27.224038812592095 23.790880783007115 94-95 21.85687418786585 26.59990649902855 27.406352377786586 24.136866935319016 96-97 21.92053271188472 26.561764477025207 27.38097973165589 24.13672307943418 98-99 21.74461515966247 26.659649024563304 27.288850193340707 24.306885622433523 100 21.758960932921383 26.552711537476743 27.21051222197566 24.477815307626216 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2313.0 1 1622.0 2 1636.5 3 2166.5 4 1581.5 5 1317.0 6 2038.0 7 3820.5 8 6154.5 9 7497.5 10 6883.5 11 5966.5 12 6304.5 13 7142.5 14 8079.0 15 9285.5 16 10427.5 17 11418.0 18 12559.5 19 13801.0 20 14932.5 21 16438.5 22 18863.0 23 22192.0 24 26200.5 25 31735.0 26 39531.0 27 49109.5 28 60954.5 29 78009.0 30 100331.5 31 123145.5 32 151617.5 33 184459.0 34 215875.0 35 246285.5 36 280869.5 37 319336.0 38 340307.0 39 345747.0 40 345743.0 41 344977.5 42 352267.0 43 367690.0 44 386596.5 45 413905.0 46 437319.5 47 450152.5 48 463931.5 49 468599.0 50 461698.5 51 452464.5 52 439683.0 53 422065.0 54 403501.0 55 373369.5 56 334217.5 57 290629.0 58 248725.5 59 216310.5 60 181404.0 61 145581.0 62 116442.5 63 91369.0 64 67587.5 65 48163.5 66 34988.0 67 26417.0 68 20259.5 69 15176.0 70 11685.5 71 8772.5 72 6636.0 73 5120.0 74 3970.0 75 2873.5 76 1884.0 77 1148.5 78 706.0 79 428.0 80 253.5 81 163.0 82 116.0 83 69.5 84 36.0 85 25.0 86 18.0 87 18.5 88 13.0 89 8.0 90 5.5 91 4.0 92 3.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029603657593662562 2 0.0019018861378096031 3 1.333097760146918E-4 4 0.023320323484170086 5 6.043376512666029E-4 6 1.7774636801958905E-5 7 0.0 8 0.00407039182764859 9 0.01006933174830972 10-11 0.00130199214574349 12-13 1.2442245761371234E-4 14-15 1.2886611681420208E-4 16-17 3.821546912421165E-4 18-19 3.1105614403428085E-5 20-21 9.776050241077398E-5 22-23 2.5773223362840416E-4 24-25 4.4436592004897265E-5 26-27 6.66548880073459E-5 28-29 9.953796609096987E-4 30-31 0.0025462167218806135 32-33 0.009767162922676419 34-35 0.018921100875685257 36-37 0.021880577903211417 38-39 0.02112071217992767 40-41 0.004670285819714703 42-43 0.00520352492377347 44-45 0.009153937953008836 46-47 0.012100084002933525 48-49 1.212998982935282 50-51 0.018036812694787803 52-53 0.018978868445291622 54-55 0.009789381218678868 56-57 0.007816396533661429 58-59 0.008096347063292283 60-61 0.005136870035766124 62-63 0.007696417735248207 64-65 0.019654304643766062 66-67 0.0294125802480415 68-69 0.029803622257684594 70-71 0.02698189866537362 72-73 0.02339142203137792 74-75 0.009651627783463687 76-77 0.014788497819229812 78-79 0.008496276391336358 80-81 0.002795061637108038 82-83 0.014512990948799447 84-85 0.02450678049070084 86-87 0.023689147197810734 88-89 0.03301194420043818 90-91 0.02095629678950955 92-93 0.013468731036684362 94-95 0.006825460531952221 96-97 0.0037993286164187162 98-99 0.015734997228934122 100 0.036011414160768744 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.1251988E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.236480285063216 #Duplication Level Percentage of deduplicated Percentage of total 1 71.52752138410754 38.794010033885876 2 15.665918478565768 16.993285574202798 3 5.707429364096283 9.286526405525967 4 2.576936424743976 5.5905584638595185 5 1.4292623815655092 3.8759080489980122 6 0.8235563535105477 2.680007875848802 7 0.5113966154761718 1.9415446717185012 8 0.35403306641969584 1.5361205941705849 9 0.2543964037110316 1.2417808981017915 >10 1.0590955573134162 9.747378159099766 >50 0.05392345267449337 2.0265199404915615 >100 0.03309723128735296 3.44532074479543 >500 0.0023307165467093653 0.8812047002880378 >1k 0.0010023363528765359 0.8683976698461083 >5k 2.5058407147063334E-5 0.08404549515398776 >10k+ 7.517522144119001E-5 1.0073907240134274 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32326 0.28729145463006184 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26235 0.23315879824969596 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15562 0.13830444895604224 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.5549273603917816E-5 2 0.0 0.0 0.0 0.0 3.5549273603917816E-5 3 0.0 0.0 0.0 0.0 3.5549273603917816E-5 4 0.0 0.0 0.0 0.0 3.5549273603917816E-5 5 0.0 0.0 0.0 0.0 3.5549273603917816E-5 6 0.0 0.0 0.0 0.0 5.332391040587672E-5 7 0.0 0.0 0.0 0.0 5.332391040587672E-5 8 0.0 0.0 0.0 0.0 6.221122880685617E-5 9 0.0 0.0 0.0 0.0 6.221122880685617E-5 10-11 0.0 0.0 0.0 8.887318400979454E-6 6.221122880685617E-5 12-13 0.0 0.0 0.0 1.333097760146918E-5 6.221122880685617E-5 14-15 0.0 0.0 0.0 2.666195520293836E-5 1.1109148001224318E-4 16-17 0.0 0.0 0.0 3.1105614403428085E-5 1.688590496186096E-4 18-19 0.0 0.0 0.0 4.443659200489727E-5 1.866336864205685E-4 20-21 0.0 0.0 0.0 1.2442245761371234E-4 1.9996466402203773E-4 22-23 0.0 0.0 0.0 3.4216175843770896E-4 2.3551393762595552E-4 24-25 0.0 0.0 0.0 0.0010398162529145962 2.710632112298733E-4 26-27 0.0 0.0 0.0 0.00227959716985123 2.9328150723232195E-4 28-29 0.0 4.443659200489727E-6 0.0 0.005923397714252806 2.977251664328117E-4 30-31 0.0 8.887318400979454E-6 0.0 0.0190321923556975 3.0216882563330143E-4 32-33 0.0 8.887318400979454E-6 0.0 0.051310932788054875 3.2883078083623975E-4 34-35 0.0 1.333097760146918E-5 0.0 0.10027561351825118 3.554927360391781E-4 36-37 0.0 2.666195520293836E-5 0.0 0.17334270175190375 3.554927360391781E-4 38-39 0.0 3.999293280440754E-5 0.0 0.2743781809934387 3.6882371364064734E-4 40-41 0.0 4.4436592004897265E-5 0.0 0.38596290717693615 3.821546912421165E-4 42-43 0.0 4.4436592004897265E-5 0.0 0.4966900071347392 3.9548566884358566E-4 44-45 0.0 4.4436592004897265E-5 0.0 0.6337146822410404 4.0881664644505487E-4 46-47 0.0 7.109854720783563E-5 0.0 0.7944373918635533 4.2659128324701377E-4 48-49 0.0 7.109854720783563E-5 0.0 0.9673135093994056 4.799151936528905E-4 50-51 0.0 7.109854720783563E-5 0.0 1.1507477611956216 4.799151936528905E-4 52-53 0.0 7.109854720783563E-5 0.0 1.329884994544964 4.8435885285338026E-4 54-55 0.0 7.109854720783563E-5 0.0 1.53083170725031 4.8880251205387E-4 56-57 0.0 7.109854720783563E-5 0.0 1.7646748290168812 4.976898304548494E-4 58-59 0.0 7.109854720783563E-5 0.0 2.0224737175332925 5.110208080563185E-4 60-61 0.0 7.109854720783563E-5 0.0 2.2854316943814728 5.243517856577878E-4 62-63 0.0 7.109854720783563E-5 0.0 2.552962196546957 5.510137408607261E-4 64-65 0.0 7.109854720783563E-5 0.0 2.824269809032857 5.510137408607261E-4 66-67 0.0 7.998586560881508E-5 0.0 3.127896154883919 5.732320368631747E-4 68-69 0.0 7.998586560881508E-5 0.0 3.4631746852200695 5.776756960636644E-4 70-71 0.0 7.998586560881508E-5 0.0 3.821075884545913 5.998939920661131E-4 72-73 0.0 7.998586560881508E-5 0.0 4.176119811005842 6.709925392739487E-4 74-75 0.0 7.998586560881508E-5 0.0 4.529128541551946 6.754361984744385E-4 76-77 0.0 7.998586560881508E-5 0.0 4.917304391010726 6.754361984744385E-4 78-79 0.0 8.44295248093048E-5 0.0 5.332453251816479 6.754361984744385E-4 80-81 0.0 8.887318400979453E-5 0.0 5.768682831869356 7.154291312788459E-4 82-83 8.887318400979454E-6 9.331684321028426E-5 0.0 6.216212637269076 7.776403600857022E-4 84-85 1.7774636801958908E-5 9.7760502410774E-5 0.0 6.650598098753749 7.865276784866816E-4 86-87 1.7774636801958908E-5 9.7760502410774E-5 0.0 7.099372128729607 7.909713376871713E-4 88 1.7774636801958908E-5 9.7760502410774E-5 0.0 7.454656012786363 8.176332928901097E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 17485 0.0 50.055336 1 GTATCAA 31270 0.0 41.210682 1 ATCAACG 39085 0.0 32.28196 3 CAACGCA 39320 0.0 32.201344 5 TATCAAC 40800 0.0 31.301746 2 TCAACGC 41405 0.0 30.666477 4 AACGCAG 42310 0.0 30.479689 6 ACGCAGA 48030 0.0 26.410276 7 CGCAGAG 49440 0.0 25.581161 8 GTACATG 45075 0.0 24.93486 1 TACATGG 46560 0.0 24.01329 2 ACATGGG 46515 0.0 23.201393 3 GCAGAGT 54915 0.0 22.747917 9 GTACTAG 4200 0.0 22.682133 1 GTGGTAT 8805 0.0 22.384998 1 CATGGGG 31835 0.0 21.210203 4 GAGTACT 29875 0.0 20.417662 12-13 TGGTATC 9315 0.0 20.046604 2 CTAGTAC 5870 0.0 19.98394 3 AGAGTAC 51290 0.0 19.821817 10-11 >>END_MODULE