FastQCFastQC Report
Mon 6 Jun 2016
ERR1211295_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211295_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5206371
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186770.35873355932568No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146440.28127077382691323No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92680.1780126694774537No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68800.13214578830436785No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78750.048.2673531
GTATCAA142400.041.4927671
TACCTGG52350.038.998232
ACCTGGG59650.034.1894233
GTACCTG62000.033.6128041
ATCAACG171350.033.2925533
CAACGCA173400.033.0067185
TCAACGC176450.032.463134
TATCAAC178500.032.3353962
AACGCAG183750.031.4800116
GTACATG167250.029.3085481
TACATGG171000.028.3893492
ACGCAGA205350.027.8491927
CGCAGAG210500.027.1224168
ACATGGG173850.026.8673113
GTGGTAT40800.024.7896841
CATGGGG118500.024.7046134
GCAGAGT233000.023.8185239
TGGTATC41550.022.756052
CCTGGGG58200.022.6877634
Produced by FastQC (version 0.11.5)