##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211295_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5206371 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.320680758247924 31.0 31.0 34.0 28.0 34.0 2 31.324570799891134 31.0 31.0 34.0 28.0 34.0 3 31.374949076813774 31.0 31.0 34.0 28.0 34.0 4 34.98042283194955 37.0 35.0 37.0 32.0 37.0 5 35.03060730785417 37.0 35.0 37.0 32.0 37.0 6 35.092471896451485 37.0 35.0 37.0 32.0 37.0 7 35.033150345989554 37.0 35.0 37.0 32.0 37.0 8 35.03145914880057 37.0 35.0 37.0 32.0 37.0 9 36.58781366137757 39.0 37.0 39.0 32.0 39.0 10-11 36.514550250068616 39.0 37.0 39.0 32.0 39.0 12-13 36.433501761591714 39.0 37.0 39.0 32.0 39.0 14-15 37.559463011760016 40.0 37.0 41.0 32.0 41.0 16-17 37.554133099619676 40.0 37.0 41.0 32.0 41.0 18-19 37.55919756774921 40.0 37.0 41.0 32.0 41.0 20-21 37.52166902819641 40.0 37.0 41.0 31.5 41.0 22-23 37.39264892955189 40.0 37.0 41.0 31.0 41.0 24-25 37.285468515401604 40.0 37.0 41.0 31.0 41.0 26-27 37.141828348383164 40.0 36.0 41.0 30.5 41.0 28-29 36.992299434673406 40.0 36.0 41.0 30.0 41.0 30-31 36.79905629084059 39.0 36.0 41.0 30.0 41.0 32-33 36.65732801216049 39.0 35.5 41.0 30.0 41.0 34-35 36.49305831259432 39.0 35.0 41.0 29.5 41.0 36-37 36.386092923458584 39.0 35.0 41.0 29.0 41.0 38-39 36.23285662892637 39.0 35.0 41.0 28.5 41.0 40-41 36.06795222622437 39.0 35.0 40.0 28.0 41.0 42-43 35.92212147386346 39.0 35.0 40.0 27.0 41.0 44-45 35.79076625542052 39.0 35.0 40.0 27.0 41.0 46-47 35.63092228732835 38.5 34.5 40.0 26.0 41.0 48-49 35.40183517463508 38.0 34.0 40.0 25.0 41.0 50-51 34.75721899188514 37.5 33.5 39.5 24.0 40.5 52-53 35.00590344022737 38.0 34.0 40.0 25.5 40.5 54-55 35.370426828975496 38.0 34.0 40.0 26.0 41.0 56-57 35.2839662213853 38.0 34.0 40.0 25.5 41.0 58-59 35.083321953045605 38.0 34.0 40.0 25.0 41.0 60-61 34.819070519561514 38.0 34.0 40.0 25.0 41.0 62-63 34.55337383755403 37.0 33.0 40.0 24.0 41.0 64-65 34.28653077162576 37.0 33.0 40.0 24.0 41.0 66-67 34.06703988248245 36.5 33.0 39.5 23.0 41.0 68-69 33.7298355226702 36.0 32.5 39.0 23.0 41.0 70-71 33.3806749461381 35.5 32.0 39.0 22.5 40.5 72-73 33.01287576317554 35.0 32.0 38.5 22.0 40.0 74-75 32.638710341617994 35.0 31.5 37.0 21.5 39.5 76-77 32.26871701997418 35.0 31.0 37.0 20.5 39.0 78-79 31.894481972183694 35.0 31.0 36.0 20.0 39.0 80-81 31.54429159965742 35.0 31.0 36.0 20.0 38.5 82-83 31.213982733846667 34.5 30.0 35.5 19.0 37.0 84-85 30.898374798876223 34.0 30.0 35.0 18.0 37.0 86-87 30.63102216495905 34.0 30.0 35.0 18.0 36.0 88-89 30.36189353390298 34.0 29.0 35.0 18.0 36.0 90-91 30.098501432187604 34.0 29.0 35.0 17.0 36.0 92-93 29.853086247599336 34.0 29.0 35.0 15.5 35.0 94-95 29.621313002857462 34.0 29.0 35.0 14.5 35.0 96-97 29.41822787888147 34.0 27.5 35.0 12.0 35.0 98-99 29.17222187969317 34.0 27.0 35.0 10.5 35.0 100 27.5485477312316 32.0 24.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4408277606914055 1101 2 -0.4296434527075803 1101 3 -0.4058179938275508 1101 4 -0.3571931816769407 1101 5 -0.4265370869561025 1101 6 -0.421272848890311 1101 7 -0.48154403735350826 1101 8 -0.5444843494187666 1101 9 -0.5549685674482063 1101 10-11 -0.617608448656803 1101 12-13 -0.5811897124394463 1101 14-15 -0.6548541641683769 1101 16-17 -0.6171168310793504 1101 18-19 -0.7327493680107438 1101 20-21 -0.7235561167115847 1101 22-23 -0.6642891142179081 1101 24-25 -0.7579968001635606 1101 26-27 -0.7780084460788714 1101 28-29 -0.8392195759423728 1101 30-31 -0.8432129695445099 1101 32-33 -0.7486971965926941 1101 34-35 -0.7733430868944495 1101 36-37 -0.9002298378118923 1101 38-39 -0.9371983658070278 1101 40-41 -0.8579905211084551 1101 42-43 -1.0548461330972785 1101 44-45 -0.9693437575901598 1101 46-47 -0.9618491983201949 1101 48-49 -1.0148407369356178 1101 50-51 -1.0699834873993623 1101 52-53 -0.7642783234979547 1101 54-55 -0.7650177374121085 1101 56-57 -0.7429607649003671 1101 58-59 -0.8198505157946201 1101 60-61 -0.7546498524275478 1101 62-63 -0.778475796710957 1101 64-65 -0.6572018043857426 1101 66-67 -0.7829612237511796 1101 68-69 -0.7731381848281771 1101 70-71 -0.7159165964616818 1101 72-73 -0.8180793390877668 1101 74-75 -0.9040728736348989 1101 76-77 -0.7917401640384689 1101 78-79 -0.8543658329787753 1101 80-81 -0.868731781400335 1101 82-83 -0.8737016686787875 1101 84-85 -0.8132290472154402 1101 86-87 -0.8640676958155531 1101 88-89 -0.9878910313864608 1101 90-91 -0.8586741047336375 1101 92-93 -0.9452394290009813 1101 94-95 -0.9670091054365493 1101 96-97 -1.0716932415007498 1101 98-99 -1.0974458789496104 1101 100 -1.1318375682674109 1102 1 -0.3685872546430282 1102 2 -0.3557878315530836 1102 3 -0.2927597261798738 1102 4 -0.15170636258654469 1102 5 -0.2300755092158866 1102 6 -0.2610466718118474 1102 7 -0.3373849761593988 1102 8 -0.2990456621459785 1102 9 -0.39765450618209286 1102 10-11 -0.3779935725700696 1102 12-13 -0.38284625126438954 1102 14-15 -0.5803018761569732 1102 16-17 -0.5079666721482781 1102 18-19 -0.48034963553586607 1102 20-21 -0.5159378838425965 1102 22-23 -0.546933332823734 1102 24-25 -0.6064349441244801 1102 26-27 -0.49734774669808246 1102 28-29 -0.6135043230690158 1102 30-31 -0.5401845919321602 1102 32-33 -0.6582182192088197 1102 34-35 -0.6392222877204432 1102 36-37 -0.6453528544844929 1102 38-39 -0.7919356268572884 1102 40-41 -0.8664062315136718 1102 42-43 -0.8151252013589598 1102 44-45 -0.8829127373316368 1102 46-47 -0.8797857473996231 1102 48-49 -0.7811405656173704 1102 50-51 -0.8534493272313668 1102 52-53 -0.7402878571547546 1102 54-55 -0.5737971903226153 1102 56-57 -0.6301184187811799 1102 58-59 -0.5611145099639714 1102 60-61 -0.6281915740797004 1102 62-63 -0.616780475573087 1102 64-65 -0.6932817478535682 1102 66-67 -0.5915840632614362 1102 68-69 -0.6194280186319219 1102 70-71 -0.6731240870388646 1102 72-73 -0.7684854197659519 1102 74-75 -0.7945281855777822 1102 76-77 -0.7639520592361464 1102 78-79 -0.6317305823295385 1102 80-81 -0.638068018072822 1102 82-83 -0.7582625357410286 1102 84-85 -0.7780677096613253 1102 86-87 -0.8001535044026546 1102 88-89 -0.8524372314460997 1102 90-91 -0.8758533597016225 1102 92-93 -0.8134847272091896 1102 94-95 -0.9565436777395426 1102 96-97 -1.112954854936202 1102 98-99 -1.165542672968197 1102 100 -1.1068775966554298 1103 1 -0.4832235851458968 1103 2 -0.5601367431914284 1103 3 -0.5051351933495987 1103 4 -0.4438280390644067 1103 5 -0.4151557780610844 1103 6 -0.5055432415013641 1103 7 -0.5350631183938006 1103 8 -0.5172211932001716 1103 9 -0.6802305615727917 1103 10-11 -0.6335791234237931 1103 12-13 -0.682353867115971 1103 14-15 -0.7863910600285848 1103 16-17 -0.8359017122425882 1103 18-19 -0.8390753494291019 1103 20-21 -0.9141267323435329 1103 22-23 -0.9095326309853746 1103 24-25 -0.9305272489310354 1103 26-27 -0.8762249814713812 1103 28-29 -0.7959410190120977 1103 30-31 -0.8436216694510676 1103 32-33 -0.8489103200122585 1103 34-35 -0.9984536060307505 1103 36-37 -1.054486030520927 1103 38-39 -1.0764324172220299 1103 40-41 -1.0344937733793316 1103 42-43 -1.005600448253297 1103 44-45 -1.1430668982163326 1103 46-47 -0.9520805992741543 1103 48-49 -0.9181551870219309 1103 50-51 -1.0572037316888796 1103 52-53 -0.9614281802344351 1103 54-55 -0.8985825216550936 1103 56-57 -0.9290928964249261 1103 58-59 -0.8745986626730584 1103 60-61 -0.8254849771403414 1103 62-63 -0.8740315677003849 1103 64-65 -0.9151993184662217 1103 66-67 -0.9803701475216187 1103 68-69 -0.901027535494876 1103 70-71 -0.8985881263137969 1103 72-73 -0.9973168062223934 1103 74-75 -1.0184115119492176 1103 76-77 -0.938887897658546 1103 78-79 -0.8941375792326447 1103 80-81 -0.9252495301850843 1103 82-83 -0.9384937952889665 1103 84-85 -0.9677098063169787 1103 86-87 -1.0694244121537757 1103 88-89 -1.2242468863014295 1103 90-91 -1.2070624408317094 1103 92-93 -1.2721668830503425 1103 94-95 -1.2991248471269685 1103 96-97 -1.30055340275144 1103 98-99 -1.2800553544805418 1103 100 -1.3541350362325488 1104 1 -0.3290471201402596 1104 2 -0.28344563600537853 1104 3 -0.39497610080999834 1104 4 -0.24142053553150333 1104 5 -0.1313535818787841 1104 6 -0.16308374972637552 1104 7 -0.17883673340895 1104 8 -0.21917650345089612 1104 9 -0.3143135984901164 1104 10-11 -0.3277284931453579 1104 12-13 -0.40886241383889654 1104 14-15 -0.5574348791524457 1104 16-17 -0.47595882844973403 1104 18-19 -0.5309527362024156 1104 20-21 -0.49789422520633764 1104 22-23 -0.4014136146949383 1104 24-25 -0.5107339221163585 1104 26-27 -0.5306231266888659 1104 28-29 -0.5229679563842851 1104 30-31 -0.6111240030432015 1104 32-33 -0.6400154990410485 1104 34-35 -0.6774725345520167 1104 36-37 -0.7577866341879158 1104 38-39 -0.6465087586363509 1104 40-41 -0.777934468144494 1104 42-43 -0.7636727730724431 1104 44-45 -0.7641380795728807 1104 46-47 -0.7332507017755532 1104 48-49 -0.8003115812214148 1104 50-51 -0.6989704231169966 1104 52-53 -0.6142262286045437 1104 54-55 -0.5694327490570359 1104 56-57 -0.5886226625200379 1104 58-59 -0.5710303448801568 1104 60-61 -0.6191720988425047 1104 62-63 -0.5621968966306312 1104 64-65 -0.6882500857128449 1104 66-67 -0.5972700360018735 1104 68-69 -0.6218432313273468 1104 70-71 -0.6265264674656805 1104 72-73 -0.7320524490734144 1104 74-75 -0.6489085800781353 1104 76-77 -0.6568515861699531 1104 78-79 -0.5690333784920014 1104 80-81 -0.5777786425505269 1104 82-83 -0.6952590288047134 1104 84-85 -0.6994426207885773 1104 86-87 -0.6293691950112006 1104 88-89 -0.6549807142944672 1104 90-91 -0.6431853920624562 1104 92-93 -0.6456965433474373 1104 94-95 -0.6447366634109706 1104 96-97 -0.7453747321810447 1104 98-99 -0.8369657560252293 1104 100 -0.938303001084055 1105 1 -0.3015506832198227 1105 2 -0.363359624035958 1105 3 -0.34071898036555126 1105 4 -0.17884479279504717 1105 5 -0.1131408512078309 1105 6 -0.2595149276064177 1105 7 -0.1490893631372927 1105 8 -0.19139256703455487 1105 9 -0.33597985413347686 1105 10-11 -0.380497009218665 1105 12-13 -0.3726370592081807 1105 14-15 -0.3964008269093071 1105 16-17 -0.4905927758029449 1105 18-19 -0.5026828197073527 1105 20-21 -0.45167157273635894 1105 22-23 -0.42590826510428315 1105 24-25 -0.49317670702194505 1105 26-27 -0.42999765267245493 1105 28-29 -0.4872094399783222 1105 30-31 -0.5870951687207793 1105 32-33 -0.5587460633224595 1105 34-35 -0.5777987173188137 1105 36-37 -0.6296869315185134 1105 38-39 -0.6099195229371333 1105 40-41 -0.7337969768535757 1105 42-43 -0.6981414723314288 1105 44-45 -0.724783078280339 1105 46-47 -0.7257775268537614 1105 48-49 -0.7246984704266879 1105 50-51 -0.6453123219034183 1105 52-53 -0.4812218904657328 1105 54-55 -0.44877908819604073 1105 56-57 -0.5102467596238824 1105 58-59 -0.4374028278889739 1105 60-61 -0.46328079372302255 1105 62-63 -0.5926633873285354 1105 64-65 -0.4625489038055761 1105 66-67 -0.5007024027211386 1105 68-69 -0.5139325628287637 1105 70-71 -0.5676172260573509 1105 72-73 -0.4731392213275427 1105 74-75 -0.4935609593641246 1105 76-77 -0.4126009720974224 1105 78-79 -0.4537938423493024 1105 80-81 -0.5029146658656778 1105 82-83 -0.5173666714407972 1105 84-85 -0.47860635035766563 1105 86-87 -0.5121789088144659 1105 88-89 -0.5112740355157754 1105 90-91 -0.6949828771945263 1105 92-93 -0.678044975638894 1105 94-95 -0.674442126031483 1105 96-97 -0.6928750848185956 1105 98-99 -0.6324190639327298 1105 100 -0.5901149076517349 1106 1 -0.3171303603353479 1106 2 -0.36361353413365904 1106 3 -0.42566767548331796 1106 4 -0.38392205232759835 1106 5 -0.34970296285541735 1106 6 -0.28259519237611386 1106 7 -0.33871716283140074 1106 8 -0.3354465851845063 1106 9 -0.43620121444278226 1106 10-11 -0.38685355155395484 1106 12-13 -0.36914883104938667 1106 14-15 -0.5980231148875887 1106 16-17 -0.6508787807894478 1106 18-19 -0.5963135274967115 1106 20-21 -0.6608349090606751 1106 22-23 -0.6324152867787163 1106 24-25 -0.5853482473065625 1106 26-27 -0.5685459810432718 1106 28-29 -0.6538887326764993 1106 30-31 -0.7029878656972954 1106 32-33 -0.6875212996029276 1106 34-35 -0.7249694320784599 1106 36-37 -0.6812946119636862 1106 38-39 -0.784206150646753 1106 40-41 -0.7367543679844104 1106 42-43 -0.7090629593783007 1106 44-45 -0.7775608112528261 1106 46-47 -0.713929521679141 1106 48-49 -0.7065034830578512 1106 50-51 -0.8054858706332269 1106 52-53 -0.6541818333839942 1106 54-55 -0.7212504490037404 1106 56-57 -0.6730410103453295 1106 58-59 -0.6811108957962446 1106 60-61 -0.6935334841098353 1106 62-63 -0.6360285044307474 1106 64-65 -0.6626425660029298 1106 66-67 -0.6127731727755545 1106 68-69 -0.6619762408026162 1106 70-71 -0.5273630060479633 1106 72-73 -0.6184353781870797 1106 74-75 -0.6977023218627423 1106 76-77 -0.6796541342749265 1106 78-79 -0.6639358471444119 1106 80-81 -0.7713303520364221 1106 82-83 -0.6648379189766835 1106 84-85 -0.6929350173151541 1106 86-87 -0.7141292319653587 1106 88-89 -0.8284482535460747 1106 90-91 -0.5818674691649903 1106 92-93 -0.6154128214137877 1106 94-95 -0.8058555987803722 1106 96-97 -0.8441370415647107 1106 98-99 -0.763315020418954 1106 100 -0.9627612326338273 1107 1 -0.5505184068005455 1107 2 -0.5469616306391529 1107 3 -0.509613016509121 1107 4 -0.4243528349215353 1107 5 -0.3959026654395501 1107 6 -0.41452075613227635 1107 7 -0.4887950058008954 1107 8 -0.44961416185623193 1107 9 -0.549835980333242 1107 10-11 -0.5137640197279083 1107 12-13 -0.568973486918189 1107 14-15 -0.7884667772860396 1107 16-17 -0.7885722230421806 1107 18-19 -0.7273837646118224 1107 20-21 -0.7734694505092534 1107 22-23 -0.7621837002558465 1107 24-25 -0.7866951762977052 1107 26-27 -0.768391640366886 1107 28-29 -0.8870929555165574 1107 30-31 -0.8031610660874335 1107 32-33 -0.828268010609456 1107 34-35 -0.8926351081111008 1107 36-37 -0.8839119028061688 1107 38-39 -0.9378271983303179 1107 40-41 -0.8875443756168195 1107 42-43 -0.9033238261058258 1107 44-45 -0.91092801916637 1107 46-47 -0.8605135874862597 1107 48-49 -0.9308300142227282 1107 50-51 -0.950586849515723 1107 52-53 -0.8587382551729448 1107 54-55 -0.7033001327628838 1107 56-57 -0.7088728608521109 1107 58-59 -0.7173165557239258 1107 60-61 -0.7160751816090496 1107 62-63 -0.8556186263497239 1107 64-65 -0.7305760274052631 1107 66-67 -0.6651586097784659 1107 68-69 -0.7311369288619076 1107 70-71 -0.8597422878235506 1107 72-73 -0.8054136441244921 1107 74-75 -0.739346357740807 1107 76-77 -0.7633869238097226 1107 78-79 -0.7617251554138527 1107 80-81 -0.8052141755053341 1107 82-83 -0.7471217305031352 1107 84-85 -0.655266232424033 1107 86-87 -0.6865266999554862 1107 88-89 -0.709111396839802 1107 90-91 -0.8079345965537215 1107 92-93 -0.7852850752771694 1107 94-95 -0.7488815144398693 1107 96-97 -0.8621122234659104 1107 98-99 -0.8681026451139466 1107 100 -0.7756111636435534 1108 1 -0.527691463929834 1108 2 -0.49068662987727407 1108 3 -0.500460611766389 1108 4 -0.33367914518510133 1108 5 -0.3319476567344424 1108 6 -0.3371141209829105 1108 7 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2208 62-63 1.071792234564363 2208 64-65 1.1002810532606944 2208 66-67 1.0779585909400282 2208 68-69 1.0072975691078057 2208 70-71 1.0259269087918597 2208 72-73 1.0074314866497431 2208 74-75 1.0049551101075238 2208 76-77 1.0117422369661782 2208 78-79 0.9370086784966922 2208 80-81 0.9981585697490232 2208 82-83 0.9638717048729966 2208 84-85 0.9795902251133484 2208 86-87 1.0451052595070855 2208 88-89 1.0137978786750033 2208 90-91 0.9849673115921007 2208 92-93 1.0965113243282119 2208 94-95 1.13796654020312 2208 96-97 1.1301895250375544 2208 98-99 1.0959040723385236 2208 100 1.162425835714842 2209 1 0.5118653617388063 2209 2 0.3804322588231983 2209 3 0.4707366705869802 2209 4 0.4093773177043971 2209 5 0.4110316634647049 2209 6 0.4639142628939439 2209 7 0.512640708907675 2209 8 0.46332695499529564 2209 9 0.6414729379922406 2209 10-11 0.6362276629823427 2209 12-13 0.6861687990019973 2209 14-15 0.829303282082364 2209 16-17 0.7903586236545905 2209 18-19 0.7982669834015397 2209 20-21 0.8353270922684146 2209 22-23 0.8476317937562001 2209 24-25 0.9338165021693285 2209 26-27 0.9452422254794257 2209 28-29 0.963827067249774 2209 30-31 0.9721015028923006 2209 32-33 0.9631403389505877 2209 34-35 1.0004147008675872 2209 36-37 0.9865902763703644 2209 38-39 1.0502142004633512 2209 40-41 1.1321289613297765 2209 42-43 1.1339525818052252 2209 44-45 1.2386565225546278 2209 46-47 1.1856398457997201 2209 48-49 1.1318860297093423 2209 50-51 1.130946396569307 2209 52-53 1.140868970973635 2209 54-55 1.1032604175393743 2209 56-57 1.1096873277246218 2209 58-59 1.122319105698054 2209 60-61 1.1612901267423084 2209 62-63 1.1580140874620568 2209 64-65 1.17638258412277 2209 66-67 1.1808142404302089 2209 68-69 1.1355370162656868 2209 70-71 1.1475420997578993 2209 72-73 1.1078370237392292 2209 74-75 1.0491082718431315 2209 76-77 1.1189342140061669 2209 78-79 1.050989606630079 2209 80-81 1.0524994381666062 2209 82-83 1.0857861658012347 2209 84-85 1.0569155704780435 2209 86-87 1.111574004643483 2209 88-89 1.1493534365736764 2209 90-91 1.189881815223643 2209 92-93 1.2148259162576416 2209 94-95 1.2199437153294213 2209 96-97 1.2336815619537411 2209 98-99 1.2602549611873748 2209 100 1.3593080950508636 2210 1 0.6483581680888477 2210 2 0.6746684952236137 2210 3 0.7456119413253255 2210 4 0.6219696950436955 2210 5 0.6075411889813722 2210 6 0.5935428540734762 2210 7 0.6308462379665443 2210 8 0.6101385578419425 2210 9 0.7533864949090372 2210 10-11 0.7519157684092548 2210 12-13 0.7841050397026734 2210 14-15 1.0233949221100858 2210 16-17 0.9849938472888411 2210 18-19 0.952039786955325 2210 20-21 0.9747698067030228 2210 22-23 1.0200319249898868 2210 24-25 1.0169338093700233 2210 26-27 1.0151924596493203 2210 28-29 1.0646294932468834 2210 30-31 1.001552448874719 2210 32-33 1.0547457511676086 2210 34-35 1.1323176913445607 2210 36-37 1.047168582446723 2210 38-39 1.065951653579127 2210 40-41 1.1405284139213094 2210 42-43 1.159478875217637 2210 44-45 1.1941637019424007 2210 46-47 1.2023334458401678 2210 48-49 1.1483529610043135 2210 50-51 1.111045466869541 2210 52-53 1.075083091268219 2210 54-55 1.0427841076714301 2210 56-57 1.0417984380073761 2210 58-59 1.0278097323113329 2210 60-61 0.9423405169750438 2210 62-63 1.0023060301073414 2210 64-65 1.0839417808205098 2210 66-67 1.0594985293189083 2210 68-69 0.9950489622474521 2210 70-71 0.9265292142008832 2210 72-73 0.9338049531708208 2210 74-75 0.9767200014503175 2210 76-77 0.9700733274343847 2210 78-79 1.009945370744834 2210 80-81 0.9652925854260133 2210 82-83 0.9330518543631676 2210 84-85 1.020909247685541 2210 86-87 1.012680158385912 2210 88-89 1.1620432532462956 2210 90-91 1.1786569785202161 2210 92-93 1.1243808459699345 2210 94-95 1.1953351852049927 2210 96-97 1.2064828251508501 2210 98-99 1.2049473686069057 2210 100 1.3565842614542198 2211 1 0.5747508195669901 2211 2 0.5674994673779885 2211 3 0.6034064225321174 2211 4 0.472136605970249 2211 5 0.4388834340998713 2211 6 0.478885039977456 2211 7 0.44140973813679096 2211 8 0.46721321254727854 2211 9 0.5196704877440794 2211 10-11 0.6030365882342679 2211 12-13 0.6472602853689082 2211 14-15 0.7299508946345483 2211 16-17 0.7665005977440487 2211 18-19 0.7182007246172404 2211 20-21 0.72497613229406 2211 22-23 0.8032429433315826 2211 24-25 0.8147819167259414 2211 26-27 0.8181589220258942 2211 28-29 0.8158304377184677 2211 30-31 0.8909267734591069 2211 32-33 0.8564539050069371 2211 34-35 0.9043667727793192 2211 36-37 0.9402780478876522 2211 38-39 0.8541709249444622 2211 40-41 0.8904284894198256 2211 42-43 0.9526934464304944 2211 44-45 1.004336756375352 2211 46-47 1.0184325783474577 2211 48-49 0.9497141547690191 2211 50-51 0.8831065762535601 2211 52-53 0.8248610368204723 2211 54-55 0.8470958499106374 2211 56-57 0.7687704665487729 2211 58-59 0.7507338933156049 2211 60-61 0.8199700117271647 2211 62-63 0.8722737211851879 2211 64-65 0.8184488569555768 2211 66-67 0.7850003723708241 2211 68-69 0.8141523063874416 2211 70-71 0.8044779122216354 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1.041386876832533 2212 32-33 0.9969584417334758 2212 34-35 0.9551641589298825 2212 36-37 1.0362467850770543 2212 38-39 1.134188109851884 2212 40-41 1.081422727710347 2212 42-43 1.1231022467852156 2212 44-45 1.19812651575441 2212 46-47 1.119571406523093 2212 48-49 1.0983324096314675 2212 50-51 1.0880720122893237 2212 52-53 1.0107184888037963 2212 54-55 0.9620890024993187 2212 56-57 0.8980036332205827 2212 58-59 0.920912170039422 2212 60-61 0.9722564239859892 2212 62-63 0.9380018865532094 2212 64-65 0.9783200645387282 2212 66-67 1.044538402707552 2212 68-69 1.0290757647146194 2212 70-71 1.0248834434995615 2212 72-73 1.0319269705829797 2212 74-75 1.1048210235551537 2212 76-77 1.0049952166953915 2212 78-79 0.9357325628312516 2212 80-81 0.9659388515954745 2212 82-83 0.9736110907461715 2212 84-85 0.9771642380679957 2212 86-87 0.9957087446625614 2212 88-89 1.0824471187670497 2212 90-91 1.0393966696163233 2212 92-93 1.0002427152880422 2212 94-95 1.085672811353792 2212 96-97 1.023047231663213 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0.6807391934436424 2213 58-59 0.7067781785643419 2213 60-61 0.7590268027270284 2213 62-63 0.7918203055288515 2213 64-65 0.74508011482191 2213 66-67 0.7112854516763818 2213 68-69 0.6854784003467032 2213 70-71 0.6839425012264115 2213 72-73 0.7239478638312562 2213 74-75 0.7764068148880412 2213 76-77 0.7501392585544764 2213 78-79 0.820035056179865 2213 80-81 0.7612940551661183 2213 82-83 0.6864881540683676 2213 84-85 0.6749682207955559 2213 86-87 0.7562483541573215 2213 88-89 0.7837911995847122 2213 90-91 0.8181149547777444 2213 92-93 0.8010719561564734 2213 94-95 0.807553499113741 2213 96-97 0.8711196141492756 2213 98-99 0.9532378866691467 2213 100 1.0963926963078627 2214 1 0.4427147776628466 2214 2 0.42506532062044045 2214 3 0.45918964381607097 2214 4 0.36240102699676413 2214 5 0.36311556629487995 2214 6 0.36511886260600335 2214 7 0.2895484777557442 2214 8 0.3031851730624737 2214 9 0.4622891864204277 2214 10-11 0.4913926803861628 2214 12-13 0.47766862587495496 2214 14-15 0.539833688657545 2214 16-17 0.5197364597488416 2214 18-19 0.5985792499078357 2214 20-21 0.6115282294396991 2214 22-23 0.7015958562277333 2214 24-25 0.709062707042392 2214 26-27 0.7057293502949022 2214 28-29 0.6886322537713809 2214 30-31 0.7427065363331522 2214 32-33 0.7420336926946831 2214 34-35 0.7331020881072092 2214 36-37 0.7409503441632737 2214 38-39 0.7222666670680482 2214 40-41 0.8101651539928341 2214 42-43 0.8617454854190285 2214 44-45 0.865866443645146 2214 46-47 0.8200665219428132 2214 48-49 0.8249374393279183 2214 50-51 0.8044842020474121 2214 52-53 0.7975148623956514 2214 54-55 0.7261116376294012 2214 56-57 0.7354456241545577 2214 58-59 0.7077080948183863 2214 60-61 0.7266761089193352 2214 62-63 0.7242442006324694 2214 64-65 0.677611239381271 2214 66-67 0.6396249487931556 2214 68-69 0.6451327190703466 2214 70-71 0.6610406327340428 2214 72-73 0.6414288630773441 2214 74-75 0.6557713848495297 2214 76-77 0.6469647572605979 2214 78-79 0.6364009200264711 2214 80-81 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0.2943631094907886 2311 8 0.38881926983843584 2311 9 0.5378452912152838 2311 10-11 0.537241529729819 2311 12-13 0.5546593437307266 2311 14-15 0.7510346003947888 2311 16-17 0.6191844742487618 2311 18-19 0.5788854508195698 2311 20-21 0.6390937262266547 2311 22-23 0.7235308262941871 2311 24-25 0.7466573135124364 2311 26-27 0.9052465689470708 2311 28-29 0.7834383722972262 2311 30-31 0.9296571597197669 2311 32-33 0.8283364883227406 2311 34-35 0.8914453077480076 2311 36-37 0.8203703474587059 2311 38-39 0.9439635418964158 2311 40-41 0.9543174031277815 2311 42-43 1.0227209344221606 2311 44-45 0.9882364112400239 2311 46-47 1.016924598506776 2311 48-49 1.00449530168855 2311 50-51 0.9881849764748267 2311 52-53 0.9283617894017411 2311 54-55 0.8127714388047735 2311 56-57 0.810918519194928 2311 58-59 0.8300264339187322 2311 60-61 0.8214981553849796 2311 62-63 0.8164364893126432 2311 64-65 0.8026576501024252 2311 66-67 0.8445801857899156 2311 68-69 0.9073859733132181 2311 70-71 0.8683183281259232 2311 72-73 0.839082787392158 2311 74-75 0.8374281652087276 2311 76-77 0.7128690797739168 2311 78-79 0.7963270473401387 2311 80-81 0.83921843453804 2311 82-83 0.8312679473569737 2311 84-85 0.8341530001970092 2311 86-87 0.865826526209954 2311 88-89 0.9382608142069699 2311 90-91 0.8849689673938883 2311 92-93 0.8019565205085293 2311 94-95 0.7876428825224693 2311 96-97 0.9160956635435902 2311 98-99 1.031833964910156 2311 100 1.035847259376009 2312 1 0.4939351783914461 2312 2 0.508958112709955 2312 3 0.5537850120179968 2312 4 0.48543251281513733 2312 5 0.44132269614135566 2312 6 0.4662794142583593 2312 7 0.5109145979743204 2312 8 0.5044133801923181 2312 9 0.6310557750230785 2312 10-11 0.6851137316481939 2312 12-13 0.6583490838504957 2312 14-15 0.8204568996905905 2312 16-17 0.7690768188432955 2312 18-19 0.7907481283881808 2312 20-21 0.7956773317035584 2312 22-23 0.7796780836542183 2312 24-25 0.801284929264618 2312 26-27 0.8370408257047401 2312 28-29 0.880196136154936 2312 30-31 0.8811868902233471 2312 32-33 0.8475424098427453 2312 34-35 0.853734406608389 2312 36-37 0.874150968675373 2312 38-39 0.8516720795062511 2312 40-41 0.8939551617992336 2312 42-43 0.8661756181278477 2312 44-45 0.8549088584252615 2312 46-47 0.8892130220729229 2312 48-49 0.875676445312827 2312 50-51 0.8951399397523261 2312 52-53 0.7658286797258569 2312 54-55 0.774943279575794 2312 56-57 0.7887047966760292 2312 58-59 0.6705262056692831 2312 60-61 0.7082959371785122 2312 62-63 0.7634362381031963 2312 64-65 0.7343884967323007 2312 66-67 0.738515138748916 2312 68-69 0.806046068016478 2312 70-71 0.7031353504025262 2312 72-73 0.6863364873292213 2312 74-75 0.6956074504232959 2312 76-77 0.7310696232507112 2312 78-79 0.7592398479473665 2312 80-81 0.7251850777044666 2312 82-83 0.7453383508317692 2312 84-85 0.7494263322878005 2312 86-87 0.8459933508954691 2312 88-89 0.8143819106687005 2312 90-91 0.8328094980130807 2312 92-93 0.8994196697026311 2312 94-95 0.993912692278581 2312 96-97 0.9583992812298163 2312 98-99 0.9118876372952158 2312 100 1.0969383727741189 2313 1 0.4483957812470756 2313 2 0.39214161350318477 2313 3 0.46403091365734284 2313 4 0.35687619340741605 2313 5 0.36295939629999907 2313 6 0.4155284888220834 2313 7 0.3398172949600422 2313 8 0.43282162979570415 2313 9 0.5386179114588288 2313 10-11 0.5247554556037741 2313 12-13 0.5526404640726028 2313 14-15 0.7157451069883152 2313 16-17 0.6666297009162747 2313 18-19 0.5496865207731716 2313 20-21 0.6141741587791785 2313 22-23 0.5313782423004412 2313 24-25 0.5812849292646192 2313 26-27 0.5858099954562022 2313 28-29 0.6686757765568458 2313 30-31 0.7151847749404254 2313 32-33 0.7412758841949412 2313 34-35 0.8050260512408514 2313 36-37 0.7946216191248681 2313 38-39 0.7996757812513593 2313 40-41 0.8875537868653325 2313 42-43 0.7815457926386884 2313 44-45 0.8473308573676235 2313 46-47 0.8740014937810088 2313 48-49 0.9796558213043696 2313 50-51 0.7667422665635328 2313 52-53 0.6715240789855059 2313 54-55 0.6644078485868974 2313 56-57 0.718863442894957 2313 58-59 0.6830460364466475 2313 60-61 0.6627552179823155 2313 62-63 0.696225767452745 2313 64-65 0.6710661804975047 2313 66-67 0.6164580261101023 2313 68-69 0.6587271891164264 2313 70-71 0.6874743244903101 2313 72-73 0.6018269685560895 2313 74-75 0.7093197719463049 2313 76-77 0.7306373123041183 2313 78-79 0.7851361990843309 2313 80-81 0.6653966059963778 2313 82-83 0.6973795988486913 2313 84-85 0.6955183470947794 2313 86-87 0.8493791256178369 2313 88-89 0.743299150224491 2313 90-91 0.8370572505249783 2313 92-93 0.8013987812838046 2313 94-95 0.8740409313055473 2313 96-97 0.9098720469622315 2313 98-99 0.89836304713128 2313 100 1.1199050570681415 2314 1 0.30845328294746466 2314 2 0.24426047977214083 2314 3 0.35646049678948444 2314 4 0.3108557264364933 2314 5 0.2876846342246395 2314 6 0.3292279119878714 2314 7 0.3824086097370696 2314 8 0.40875076900952223 2314 9 0.46795587608035305 2314 10-11 0.4657132677873861 2314 12-13 0.470533279864668 2314 14-15 0.5619230481418853 2314 16-17 0.5755399593234642 2314 18-19 0.571492917869783 2314 20-21 0.645098508347985 2314 22-23 0.6465992668950733 2314 24-25 0.692047040965889 2314 26-27 0.7375159268279816 2314 28-29 0.6960151222575846 2314 30-31 0.6985793222735381 2314 32-33 0.7578130876652907 2314 34-35 0.7257767794226524 2314 36-37 0.7899834774552161 2314 38-39 0.792372857620883 2314 40-41 0.8498294523534113 2314 42-43 0.6611278863755459 2314 44-45 0.727693105100002 2314 46-47 0.6285487749760108 2314 48-49 0.6433457690206481 2314 50-51 0.8091936821697345 2314 52-53 0.7071005237968606 2314 54-55 0.7298119051176926 2314 56-57 0.6596771893059667 2314 58-59 0.6789929583533691 2314 60-61 0.564735132625394 2314 62-63 0.6700970207561667 2314 64-65 0.7162980174886187 2314 66-67 0.67473320873966 2314 68-69 0.6813927498813186 2314 70-71 0.6093310453098155 2314 72-73 0.6880838415019923 2314 74-75 0.6876521187944817 2314 76-77 0.7157213582431723 2314 78-79 0.6961889604639531 2314 80-81 0.7963354023806488 2314 82-83 0.7813774597335161 2314 84-85 0.8874652274652846 2314 86-87 0.818324871530411 2314 88-89 0.7074921923889441 2314 90-91 0.7657243143817212 2314 92-93 0.8949074623531565 2314 94-95 0.92089055202797 2314 96-97 0.8382074571064138 2314 98-99 0.8539714488695687 2314 100 0.956453033994773 2315 1 0.2120500747715397 2315 2 0.2691203914941944 2315 3 0.2743646677996807 2315 4 0.13147534737769462 2315 5 0.21804191219487024 2315 6 0.11614621268653735 2315 7 0.06868581929484918 2315 8 0.14254038345445252 2315 9 -0.00513831795673525 2315 10-11 0.14702165208425555 2315 12-13 0.11365483436076573 2315 14-15 0.28747992604893113 2315 16-17 0.08607887804802061 2315 18-19 0.29571037199003314 2315 20-21 0.20433716712240368 2315 22-23 0.21414425552537608 2315 24-25 0.34104270108093004 2315 26-27 0.3727658028749943 2315 28-29 0.37287008850866954 2315 30-31 0.3323932841057484 2315 32-33 0.4089681116150601 2315 34-35 0.31073762993637644 2315 36-37 0.4873978895693156 2315 38-39 0.6017283195950327 2315 40-41 0.4996823508037025 2315 42-43 0.4633421550163277 2315 44-45 0.5763778267792219 2315 46-47 0.5463326059893916 2315 48-49 0.45312996445748155 2315 50-51 0.43076477645170286 2315 52-53 0.13705610543539137 2315 54-55 0.27593465988969257 2315 56-57 0.29850298943932074 2315 58-59 0.3791543251962892 2315 60-61 0.4318180568141585 2315 62-63 0.4834850536724318 2315 64-65 0.38687419741810203 2315 66-67 0.32694622394463835 2315 68-69 0.4308529787696216 2315 70-71 0.4147831151564958 2315 72-73 0.4428122852063794 2315 74-75 0.10609093668794145 2315 76-77 0.40480761014526223 2315 78-79 0.4108787912338663 2315 80-81 0.4343981433066908 2315 82-83 0.359806411169572 2315 84-85 0.35449356687416156 2315 86-87 0.325656531228514 2315 88-89 0.31739752026832235 2315 90-91 0.4112340641184673 2315 92-93 0.5498822017483569 2315 94-95 0.6038573576190096 2315 96-97 0.5430151593538675 2315 98-99 0.6372071373948138 2315 100 0.7207686487748113 2316 1 -0.03029908483803112 2316 2 0.049140735037841665 2316 3 0.0775172974445475 2316 4 -0.0351479541747608 2316 5 -0.0722493924393035 2316 6 -0.22113926741400292 2316 7 -0.1695374416025004 2316 8 -0.10288819062964194 2316 9 -0.011751043984588705 2316 10-11 -0.02923047873604645 2316 12-13 -0.04659230881346588 2316 14-15 -0.2432575284077032 2316 16-17 -0.014230939750540017 2316 18-19 0.10191705663702777 2316 20-21 0.07946287764984561 2316 22-23 -0.06960809041320459 2316 24-25 -0.055307850976042516 2316 26-27 -0.2063700797240955 2316 28-29 -0.10470580427218579 2316 30-31 -0.7564889058293573 2316 32-33 -0.529538228452914 2316 34-35 -0.5761741264951539 2316 36-37 -0.3884451442410821 2316 38-39 -0.23751639836415706 2316 40-41 -0.10129297200614218 2316 42-43 -0.009980591965693009 2316 44-45 -0.0929354402073983 2316 46-47 -0.21482953443202746 2316 48-49 -0.11093029894323791 2316 50-51 0.04416437937482698 2316 52-53 -0.2774245205772985 2316 54-55 -0.21468156079837541 2316 56-57 -0.24789992305736064 2316 58-59 -0.6140454260959984 2316 60-61 -0.18225208553767658 2316 62-63 -0.1672734868731851 2316 64-65 -0.42707959367561443 2316 66-67 -0.693756687162896 2316 68-69 -0.7710208139071284 2316 70-71 -0.5786810617279983 2316 72-73 -0.6867622263202122 2316 74-75 -0.533288146499423 2316 76-77 -0.21341256614079285 2316 78-79 -0.13024337966089305 2316 80-81 -0.1983847787101567 2316 82-83 -0.06358242284735738 2316 84-85 -0.0034007319466695662 2316 86-87 0.09177268830553231 2316 88-89 0.10196260606628726 2316 90-91 -0.5091845010940546 2316 92-93 -0.48940169472183115 2316 94-95 -0.2419173620201569 2316 96-97 -0.002258844373645985 2316 98-99 0.06836621369239637 2316 100 -0.05539065888111239 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 144.0 1 387.0 2 229.0 3 206.0 4 205.0 5 202.0 6 217.0 7 901.0 8 2463.0 9 3703.0 10 4954.0 11 7711.0 12 11499.0 13 14570.0 14 16458.0 15 18662.0 16 21877.0 17 26799.0 18 32311.0 19 37852.0 20 43978.0 21 50979.0 22 56268.0 23 57964.0 24 58375.0 25 59676.0 26 64762.0 27 73652.0 28 85598.0 29 101758.0 30 121948.0 31 149867.0 32 187588.0 33 242848.0 34 330206.0 35 459340.0 36 670794.0 37 990978.0 38 966090.0 39 232140.0 40 212.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.810401387107774 18.76082477197735 12.891360835338588 23.537413005576287 2 15.977677822883962 20.33314437961392 40.57702927834036 23.112148519161753 3 18.989605205273662 26.29965670859035 29.801459612831838 24.90927847330415 4 12.559469001965825 16.684189991130438 38.417537181753794 32.338803825149945 5 13.083418038063627 36.94506234658774 34.735772150775254 15.235747464573379 6 29.86751847771634 36.56636441671178 19.34428535439571 14.22183175117617 7 26.863989807810746 32.452975940553245 22.75244836409021 17.930585887545796 8 25.540282259327412 35.06603978239028 20.992156004490848 18.401521953791462 9 26.46295765790743 16.455424052088517 21.024543492499728 36.05707479750432 10-11 24.67166196186115 26.018062879310232 28.885369878606316 20.4249052802223 12-13 25.180392800351083 24.070540434810344 28.68979509176332 22.059271673075255 14-15 22.761139938930814 25.452238122754157 26.782887370725124 25.00373456758991 16-17 22.054253333118133 28.03360440977658 27.46853837764553 22.443603879459754 18-19 21.65265340025782 27.286211303238577 29.437187972730378 21.623947323773223 20-21 22.5990118987225 26.657183430952053 29.29498339713139 21.448821273194056 22-23 22.431218999259787 26.512766943612377 29.107028794513663 21.948985262614173 24-25 21.90939761231362 26.534030022949317 29.418690592412645 22.137881772324413 26-27 21.9407851464055 26.656783334888413 29.169816466182947 22.232615052523137 28-29 21.898080515869765 26.963990119516644 29.197466310157548 21.94046305445604 30-31 22.450336515369347 26.729083292874495 29.199908490802528 21.62067170095363 32-33 22.24835106766267 26.862700261811547 28.961093363437655 21.927855307088127 34-35 22.365146914578162 26.95370721502811 28.978126027142455 21.703019843251276 36-37 22.284889580925263 26.913055228608187 28.80513015568993 21.99692503477662 38-39 22.257688226916585 27.096078828687958 28.771698627865305 21.874534316530152 40-41 22.44147101916067 26.701368443547285 28.992206513366963 21.864954023925083 42-43 22.14994543046704 26.876268508623024 28.736850318432776 22.236935742477158 44-45 22.08450353961475 27.14801058285762 28.74630728589561 22.021178591632015 46-47 22.050770289281292 26.880405285937226 28.42460659127774 22.644217833503742 48-49 22.0844649127578 26.903374087809727 28.500599526490344 22.511561472942127 50-51 22.112304233706144 27.24645749824254 28.300963615825115 22.340274652226203 52-53 22.104244525571996 27.363846816918137 28.093591169034347 22.43831748847552 54-55 21.963007717150802 27.18485418355358 28.39613823458309 22.45599986471253 56-57 22.225538368819734 27.25320630591433 28.00312520659188 22.51813011867406 58-59 22.048883409333815 27.223277103835425 28.37269609579779 22.35514339103297 60-61 22.294785969666517 27.407987177194748 28.13633204701093 22.160894806127803 62-63 22.3287879469236 27.290786733129398 28.108666098589453 22.271759221357552 64-65 22.1037730944971 27.067208458923623 28.141437258694403 22.687581187884874 66-67 22.08667098859934 27.062591443514943 28.049304623324545 22.801432944561174 68-69 21.843916375495038 27.13465591652232 28.207396198218742 22.8140315097639 70-71 22.00159300438725 26.872796322310393 28.264066277746053 22.861544395556308 72-73 21.99402623532612 27.272783181823907 27.90262973065976 22.830560852190214 74-75 22.132964649004588 27.265361051661223 27.780539686059747 22.821134613274445 76-77 21.998957088927146 27.167775796603323 27.88293952566667 22.95032758880286 78-79 21.93702533427556 27.15290220547975 27.874679504242238 23.03539295600245 80-81 22.023539143322324 27.35819294471456 27.698339033505405 22.919928878457714 82-83 22.368000676445337 27.217047191190453 27.916466687709494 22.498485444654712 84-85 22.41152072616828 27.412787200423104 27.49320543288628 22.682486640522335 86-87 22.074039112167142 27.468657436936766 27.76647352277206 22.690829928124028 88-89 22.175814120561828 27.181767766210026 27.719810894032793 22.92260721919535 90-91 22.48218579932369 27.14349182839284 27.724457679194565 22.649864693088904 92-93 22.465248909368793 27.259941723441894 27.60044412353692 22.67436524365239 94-95 22.21212971630565 27.229275550079485 27.648224003013976 22.910370730600892 96-97 22.264889572642872 27.186534326442423 27.587464544517786 22.961111556396922 98-99 22.30127451314905 27.20767677796771 27.403431156058055 23.087617552825186 100 22.546413640052464 27.192758433686283 27.208181569929113 23.05264635633214 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2170.0 1 1601.5 2 3051.5 3 4208.0 4 2463.5 5 1679.0 6 2272.0 7 3723.0 8 5801.5 9 6797.0 10 5765.0 11 4540.0 12 4450.0 13 4918.5 14 5429.5 15 5895.5 16 6291.5 17 6771.0 18 7329.0 19 7901.5 20 8573.0 21 9608.0 22 11278.0 23 13566.0 24 16298.0 25 19848.0 26 24738.5 27 30699.0 28 37488.0 29 45926.0 30 56455.5 31 67231.5 32 79100.5 33 92400.5 34 104981.0 35 117444.0 36 131439.0 37 145710.0 38 155329.0 39 160595.0 40 163398.0 41 165203.5 42 168463.0 43 173889.5 44 179942.0 45 188099.0 46 196502.0 47 200737.5 48 204655.5 49 208013.0 50 206682.0 51 201263.5 52 193046.0 53 183767.5 54 173439.0 55 159159.0 56 143766.5 57 127723.5 58 111437.0 59 96476.0 60 78523.5 61 61465.5 62 48734.0 63 37779.0 64 28084.5 65 20395.5 66 15323.0 67 11779.0 68 9209.5 69 7090.0 70 5372.0 71 4057.5 72 3115.5 73 2453.0 74 1874.5 75 1395.0 76 1024.5 77 687.5 78 472.0 79 317.5 80 215.5 81 144.0 82 116.0 83 96.0 84 69.5 85 51.0 86 35.5 87 30.0 88 23.0 89 17.5 90 16.0 91 12.0 92 12.5 93 10.5 94 7.0 95 4.5 96 2.5 97 4.0 98 4.0 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024892578727101856 2 0.11145959440846609 3 0.01692157550816106 4 0.017824315631751943 5 0.019860282718999474 6 0.04609736801315158 7 0.04582846669974153 8 0.03856813123767016 9 0.03249864444927186 10-11 0.037271642762300265 12-13 0.0363977134937176 14-15 0.031086912553869094 16-17 0.03716600296060346 18-19 0.03645533520373404 20-21 0.047076937083431046 22-23 0.04730742392349681 24-25 0.0650068925168798 26-27 0.05757369192475911 28-29 0.0494970489041215 30-31 0.04845025450548952 32-33 0.04313945356564102 34-35 0.044359113094322326 36-37 0.04003748484308936 38-39 0.04468563611774881 40-41 0.053684226498649436 42-43 0.056046716609323456 44-45 0.05859167546838287 46-47 0.06013785802049067 48-49 0.05950401921030983 50-51 0.054644588332256765 52-53 0.06369119680483777 54-55 0.0506494831044503 56-57 0.05522080543242116 58-59 0.05350175775026405 60-61 0.04835421832212879 62-63 0.05479824622563394 64-65 0.05334809985688688 66-67 0.05148499789968867 68-69 0.057007078442930785 70-71 0.05753527745141481 72-73 0.05991697479876098 74-75 0.05074551928781103 76-77 0.051235303822950766 78-79 0.059619262630342715 80-81 0.06080050768567972 82-83 0.0518307281597873 84-85 0.056642140946160005 86-87 0.05424123636214169 88-89 0.06254836622284504 90-91 0.0685602313012269 92-93 0.07230564245229547 94-95 0.07569571972492932 96-97 0.07145092042038495 98-99 0.08044951080128558 100 0.12275344957168823 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5206371.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.45952650199084 #Duplication Level Percentage of deduplicated Percentage of total 1 78.9750054575716 52.48641468202351 2 13.721286456666014 18.23820401816406 3 3.7139054663850306 7.404731963073137 4 1.404393982324603 3.7334143635015367 5 0.6789196323977884 2.256033865103135 6 0.37574884632058025 1.498325424608105 7 0.24630008343879847 1.1458290845920367 8 0.15849312217532094 0.842670228287521 9 0.118774808910282 0.7104345804487605 >10 0.5596061457896123 6.493962730275128 >50 0.02981608258677617 1.3524348076474626 >100 0.01662071580557751 1.996892278858109 >500 6.843636500331162E-4 0.30227653596852816 >1k 3.079633693117796E-4 0.4133899315853468 >5k 6.843630429146326E-5 0.36722525114082505 >10k+ 6.843630429146326E-5 0.7577602547227787 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18677 0.35873355932568 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14644 0.28127077382691323 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9268 0.1780126694774537 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6880 0.13214578830436785 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.841447334429298E-5 2 0.0 0.0 0.0 0.0 3.841447334429298E-5 3 0.0 0.0 0.0 0.0 3.841447334429298E-5 4 0.0 0.0 0.0 0.0 3.841447334429298E-5 5 0.0 0.0 0.0 0.0 3.841447334429298E-5 6 0.0 0.0 0.0 0.0 3.841447334429298E-5 7 0.0 0.0 0.0 0.0 3.841447334429298E-5 8 0.0 0.0 0.0 0.0 3.841447334429298E-5 9 0.0 0.0 0.0 0.0 5.762171001643947E-5 10-11 0.0 0.0 0.0 9.603618336073246E-6 5.762171001643947E-5 12-13 0.0 0.0 0.0 1.920723667214649E-5 5.762171001643947E-5 14-15 0.0 0.0 0.0 2.881085500821974E-5 6.722532835251272E-5 16-17 0.0 0.0 0.0 3.841447334429298E-5 9.603618336073245E-5 18-19 0.0 0.0 0.0 1.248470383689522E-4 9.603618336073245E-5 20-21 0.0 0.0 0.0 2.592976950739776E-4 9.603618336073245E-5 22-23 0.0 9.603618336073246E-6 0.0 7.106677568694201E-4 1.248470383689522E-4 24-25 0.0 1.920723667214649E-5 0.0 0.0014405427504109868 1.920723667214649E-4 26-27 0.0 1.920723667214649E-5 0.0 0.002612184187411923 1.920723667214649E-4 28-29 0.0 1.920723667214649E-5 0.0 0.005973450605037558 1.920723667214649E-4 30-31 0.0 1.920723667214649E-5 0.0 0.016681485049759227 1.920723667214649E-4 32-33 0.0 1.920723667214649E-5 0.0 0.04265927264883736 1.920723667214649E-4 34-35 0.0 1.920723667214649E-5 0.0 0.08122740388650751 1.920723667214649E-4 36-37 0.0 1.920723667214649E-5 0.0 0.14243126354230232 2.0167598505753815E-4 38-39 1.920723667214649E-5 1.920723667214649E-5 0.0 0.2345779814769251 2.112796033936114E-4 40-41 1.920723667214649E-5 1.920723667214649E-5 0.0 0.3391517815384266 2.112796033936114E-4 42-43 1.920723667214649E-5 1.920723667214649E-5 0.0 0.4408925141907866 2.304868400657579E-4 44-45 1.920723667214649E-5 1.920723667214649E-5 0.0 0.5641741627709589 2.689013134100509E-4 46-47 1.920723667214649E-5 1.920723667214649E-5 0.0 0.7103988555560101 2.9771216841827065E-4 48-49 1.920723667214649E-5 1.920723667214649E-5 0.0 0.8619343492809098 3.4573026009863684E-4 50-51 1.920723667214649E-5 1.920723667214649E-5 0.0 1.024168273832195 3.4573026009863684E-4 52-53 1.920723667214649E-5 1.920723667214649E-5 0.0 1.1908198628180742 3.6493749677078333E-4 54-55 1.920723667214649E-5 1.920723667214649E-5 0.0 1.380011144038717 3.9374835177900303E-4 56-57 1.920723667214649E-5 1.920723667214649E-5 0.0 1.5929521734044694 4.033519701150763E-4 58-59 1.920723667214649E-5 1.920723667214649E-5 0.0 1.8326776943095295 4.033519701150763E-4 60-61 1.920723667214649E-5 1.920723667214649E-5 0.0 2.0719806560078027 4.033519701150763E-4 62-63 1.920723667214649E-5 1.920723667214649E-5 0.0 2.3194947113834186 4.225592067872228E-4 64-65 1.920723667214649E-5 1.920723667214649E-5 0.0 2.565376151641902 4.225592067872228E-4 66-67 1.920723667214649E-5 1.920723667214649E-5 0.0 2.866046234507683 4.32162825123296E-4 68-69 1.920723667214649E-5 1.920723667214649E-5 0.0 3.1814578715193367 4.417664434593693E-4 70-71 1.920723667214649E-5 1.920723667214649E-5 0.0 3.503649663076258 4.417664434593693E-4 72-73 1.920723667214649E-5 5.762171001643947E-5 0.0 3.8171981981307135 4.993881534758088E-4 74-75 1.920723667214649E-5 5.762171001643947E-5 0.0 4.135750218338263 4.993881534758088E-4 76-77 1.920723667214649E-5 5.762171001643947E-5 0.0 4.492668693798425 4.993881534758088E-4 78-79 1.920723667214649E-5 5.762171001643947E-5 0.0 4.873903530885524 4.993881534758088E-4 80-81 1.920723667214649E-5 5.762171001643947E-5 0.0 5.26999516553853 4.993881534758088E-4 82-83 1.920723667214649E-5 5.762171001643947E-5 0.0 5.665491375854698 5.570098634922482E-4 84-85 1.920723667214649E-5 5.762171001643947E-5 0.0 6.066461648622428 5.570098634922482E-4 86-87 1.920723667214649E-5 5.762171001643947E-5 0.0 6.481971799550973 5.666134818283215E-4 88 1.920723667214649E-5 5.762171001643947E-5 0.0 6.810578808156391 5.954243368365412E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7875 0.0 48.267353 1 GTATCAA 14240 0.0 41.492767 1 TACCTGG 5235 0.0 38.99823 2 ACCTGGG 5965 0.0 34.189423 3 GTACCTG 6200 0.0 33.612804 1 ATCAACG 17135 0.0 33.292553 3 CAACGCA 17340 0.0 33.006718 5 TCAACGC 17645 0.0 32.46313 4 TATCAAC 17850 0.0 32.335396 2 AACGCAG 18375 0.0 31.480011 6 GTACATG 16725 0.0 29.308548 1 TACATGG 17100 0.0 28.389349 2 ACGCAGA 20535 0.0 27.849192 7 CGCAGAG 21050 0.0 27.122416 8 ACATGGG 17385 0.0 26.867311 3 GTGGTAT 4080 0.0 24.789684 1 CATGGGG 11850 0.0 24.704613 4 GCAGAGT 23300 0.0 23.818523 9 TGGTATC 4155 0.0 22.75605 2 CCTGGGG 5820 0.0 22.687763 4 >>END_MODULE