FastQCFastQC Report
Mon 6 Jun 2016
ERR1211295_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211295_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5206371
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172740.3317858062746585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143150.274951592961777No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89700.17228891294915402No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56530.10857850890764412No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79100.052.2096021
GTATCAA142650.041.0898781
TACCTGG48850.040.1453172
GTACCTG59400.033.3403551
ACCTGGG58700.032.5315363
ATCAACG179100.031.9866163
CAACGCA180600.031.7930815
TCAACGC182750.031.3734324
TATCAAC185550.031.2522092
AACGCAG192250.030.2077986
ACGCAGA215500.026.643977
CGCAGAG221200.025.8937748
GTACATG168600.025.079071
TACATGG170450.024.689862
ACATGGG171050.023.4526143
GCAGAGT247100.022.7618229
CCTGGGG57500.022.6026924
TGGTATC39350.021.8187332
GTGGTAT41100.021.6948171
CATGGGG116450.021.596014
Produced by FastQC (version 0.11.5)