FastQCFastQC Report
Mon 6 Jun 2016
ERR1211294_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211294_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9758513
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT404270.4142741829620967No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315020.32281557651252807No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT184230.18878900914514332No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA177600.052.3579561
GTATCAA332400.043.0021
CAACGCA404300.034.2510875
ATCAACG408000.033.9645543
TATCAAC429650.032.6986962
GTACTAG45300.031.9794861
AACGCAG439700.031.8466996
TCAACGC437200.031.803264
ACGCAGA484850.028.6110367
CGCAGAG496300.027.8357688
GTACATG446200.026.0156421
GCAGAGT534000.025.4049919
GCATCGT49650.025.1745978
TACATGG470650.024.4537772
TAGGCAT66950.024.0736265
GTGGTAT85700.024.038771
ACATGGG464400.023.829233
TAGTACT63500.023.0140724
GAGTACT292550.022.2384312-13
TGGTATC89250.021.9227562
Produced by FastQC (version 0.11.5)