##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211294_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9758513 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.397568051607863 31.0 31.0 34.0 28.0 34.0 2 31.420060515367453 31.0 31.0 34.0 28.0 34.0 3 31.463108569922486 31.0 31.0 34.0 28.0 34.0 4 35.074286830380814 37.0 35.0 37.0 32.0 37.0 5 35.12096146205882 37.0 35.0 37.0 32.0 37.0 6 35.191465441507326 37.0 35.0 37.0 32.0 37.0 7 35.12417404168033 37.0 35.0 37.0 32.0 37.0 8 35.12670424274682 37.0 35.0 37.0 32.0 37.0 9 36.67888109592107 39.0 37.0 39.0 32.0 39.0 10-11 36.6152732491108 39.0 37.0 39.0 32.0 39.0 12-13 36.52886689806121 39.0 37.0 39.0 32.0 39.0 14-15 37.64678870643509 40.0 37.0 41.0 32.0 41.0 16-17 37.64919009689284 40.0 37.0 41.0 32.0 41.0 18-19 37.66581722030806 40.0 37.0 41.0 32.0 41.0 20-21 37.64124472652749 40.0 37.0 41.0 32.0 41.0 22-23 37.51702364899242 40.0 37.0 41.0 31.5 41.0 24-25 37.40024545747902 40.0 37.0 41.0 31.0 41.0 26-27 37.247887972276104 40.0 36.5 41.0 31.0 41.0 28-29 37.1189062821354 40.0 36.0 41.0 30.5 41.0 30-31 36.93640634592586 39.0 36.0 41.0 30.0 41.0 32-33 36.814332265581854 39.0 36.0 41.0 30.0 41.0 34-35 36.67032400325746 39.0 35.0 41.0 30.0 41.0 36-37 36.57352298449569 39.0 35.0 41.0 30.0 41.0 38-39 36.420464521592585 39.0 35.0 41.0 29.5 41.0 40-41 36.25630774893675 39.0 35.0 40.0 29.0 41.0 42-43 36.1040939844011 39.0 35.0 40.0 28.0 41.0 44-45 35.94329028408325 39.0 35.0 40.0 27.5 41.0 46-47 35.753011652492546 38.5 35.0 40.0 27.0 41.0 48-49 35.49540052874859 38.0 34.0 40.0 26.0 41.0 50-51 34.81774313361062 37.5 33.5 39.5 25.0 40.5 52-53 35.053453687052524 38.0 34.0 40.0 26.0 40.5 54-55 35.405557281114454 38.0 34.0 40.0 26.0 41.0 56-57 35.29465559967999 38.0 34.0 40.0 26.0 41.0 58-59 35.0655034224989 38.0 34.0 40.0 26.0 41.0 60-61 34.80232413483489 37.5 34.0 40.0 25.5 41.0 62-63 34.527055095381854 37.0 33.0 40.0 25.0 41.0 64-65 34.25093177618352 36.5 33.0 40.0 25.0 41.0 66-67 34.03005165848526 36.0 33.0 39.0 24.5 41.0 68-69 33.69888747394198 35.5 33.0 39.0 23.5 41.0 70-71 33.375704423409594 35.0 32.0 39.0 23.5 40.5 72-73 33.02820726887386 35.0 32.0 38.0 23.0 40.0 74-75 32.66060536067329 35.0 32.0 37.0 22.0 39.5 76-77 32.31730561818179 35.0 31.0 37.0 21.5 39.0 78-79 31.957552651720604 35.0 31.0 36.0 20.5 39.0 80-81 31.629049015972 35.0 31.0 36.0 20.0 37.5 82-83 31.31741618830656 34.5 30.5 35.0 20.0 37.0 84-85 31.014521218550406 34.0 30.0 35.0 19.0 37.0 86-87 30.766956656203668 34.0 30.0 35.0 18.5 36.0 88-89 30.51286650947742 34.0 29.5 35.0 18.0 36.0 90-91 30.253955956199473 34.0 29.0 35.0 18.0 36.0 92-93 30.017382054007612 34.0 29.0 35.0 17.0 35.0 94-95 29.803484096398705 34.0 29.0 35.0 16.0 35.0 96-97 29.605373994992885 34.0 29.0 35.0 15.0 35.0 98-99 29.370869670409824 34.0 27.5 35.0 13.5 35.0 100 27.69515519423912 32.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.42909358988900337 1101 2 -0.41610137714070206 1101 3 -0.3600181251548378 1101 4 -0.29789560570864637 1101 5 -0.289668329485238 1101 6 -0.3651303340456593 1101 7 -0.39354905094992887 1101 8 -0.45291830087654006 1101 9 -0.5249166636835838 1101 10-11 -0.549748996552573 1101 12-13 -0.5741802978103223 1101 14-15 -0.6168901423762847 1101 16-17 -0.6104180867848541 1101 18-19 -0.7372829088663693 1101 20-21 -0.6688766937777686 1101 22-23 -0.6821493384065178 1101 24-25 -0.6082348102363397 1101 26-27 -0.7002447114089634 1101 28-29 -0.7689740831796072 1101 30-31 -0.8482747299572893 1101 32-33 -0.7881938504495736 1101 34-35 -0.8322377333895687 1101 36-37 -0.9513988274588243 1101 38-39 -0.894757649296281 1101 40-41 -0.8700998375493754 1101 42-43 -1.0005574282529324 1101 44-45 -0.9960902535288483 1101 46-47 -0.8860644738701282 1101 48-49 -0.891100990440691 1101 50-51 -0.9137744430316275 1101 52-53 -0.7250233528121157 1101 54-55 -0.6377555061627831 1101 56-57 -0.6765188459084754 1101 58-59 -0.7377889248922074 1101 60-61 -0.6587683268671256 1101 62-63 -0.7268573896346737 1101 64-65 -0.7214674564663852 1101 66-67 -0.7600719095511792 1101 68-69 -0.7580359732574493 1101 70-71 -0.698796447390734 1101 72-73 -0.7819587320770651 1101 74-75 -0.836602120955277 1101 76-77 -0.7400195891605392 1101 78-79 -0.8992417001320163 1101 80-81 -0.9752501773575339 1101 82-83 -1.0466995177569807 1101 84-85 -0.9432378138219164 1101 86-87 -0.909815852181854 1101 88-89 -0.9493049076008404 1101 90-91 -1.1083784425461225 1101 92-93 -1.0588061107745617 1101 94-95 -1.0744261457110333 1101 96-97 -1.1161822477583812 1101 98-99 -1.072692321678467 1101 100 -1.1615490835914422 1102 1 -0.3665033881657713 1102 2 -0.36048601717106976 1102 3 -0.3558267965203683 1102 4 -0.2789590576037426 1102 5 -0.2588419187579305 1102 6 -0.3210297747861546 1102 7 -0.3092895727346203 1102 8 -0.3206283094687734 1102 9 -0.3928673801404656 1102 10-11 -0.3957836305632796 1102 12-13 -0.43988027024146703 1102 14-15 -0.5663043336304057 1102 16-17 -0.5719073382649569 1102 18-19 -0.5279778910603383 1102 20-21 -0.5282785751265919 1102 22-23 -0.5999214259413534 1102 24-25 -0.6485792993478228 1102 26-27 -0.6649484011256206 1102 28-29 -0.6829292961441027 1102 30-31 -0.5680937559178929 1102 32-33 -0.5780961202329706 1102 34-35 -0.7154626344573813 1102 36-37 -0.6829839503166966 1102 38-39 -0.8343573283524606 1102 40-41 -0.7984599534562165 1102 42-43 -0.7500362670982668 1102 44-45 -0.7736851082315752 1102 46-47 -0.8102301238617571 1102 48-49 -0.7406293198896705 1102 50-51 -0.6354183663737416 1102 52-53 -0.6376737511095172 1102 54-55 -0.59126049581171 1102 56-57 -0.5652063605899684 1102 58-59 -0.5627806012942571 1102 60-61 -0.7090581331753327 1102 62-63 -0.6796512165642241 1102 64-65 -0.7140813368159797 1102 66-67 -0.6013334258224887 1102 68-69 -0.6301222531836714 1102 70-71 -0.6567710032596281 1102 72-73 -0.6856145268894878 1102 74-75 -0.6770460422581586 1102 76-77 -0.670700410453577 1102 78-79 -0.6532843608754959 1102 80-81 -0.6411161903717613 1102 82-83 -0.6733387567467908 1102 84-85 -0.7381137716239223 1102 86-87 -0.8424872192793877 1102 88-89 -0.9388623706230845 1102 90-91 -0.8597621009997845 1102 92-93 -0.8455755116206056 1102 94-95 -0.9779961555032664 1102 96-97 -0.9598423720182296 1102 98-99 -0.9326733646596068 1102 100 -0.9976374200051907 1103 1 -0.42059719964669995 1103 2 -0.4796735055317569 1103 3 -0.46260508469382344 1103 4 -0.3865474551413044 1103 5 -0.3188040513145296 1103 6 -0.4140053324261359 1103 7 -0.411481912268826 1103 8 -0.38621076506289853 1103 9 -0.5388158982237741 1103 10-11 -0.5506486724806123 1103 12-13 -0.6330451914392015 1103 14-15 -0.70633281060109 1103 16-17 -0.6676220427969568 1103 18-19 -0.6249230899473943 1103 20-21 -0.7047642782965156 1103 22-23 -0.6948151292145326 1103 24-25 -0.6508328415842684 1103 26-27 -0.6570561631876402 1103 28-29 -0.6503352287629198 1103 30-31 -0.6982804511589933 1103 32-33 -0.7764505485454691 1103 34-35 -0.87697117899733 1103 36-37 -0.9079471984035052 1103 38-39 -0.965613668238575 1103 40-41 -0.8625449381561978 1103 42-43 -0.875044478823007 1103 44-45 -0.9212667388338147 1103 46-47 -0.8485717005287086 1103 48-49 -0.8668323737820316 1103 50-51 -0.9485070258699295 1103 52-53 -0.8022598551591145 1103 54-55 -0.7655275055401347 1103 56-57 -0.6950667862931539 1103 58-59 -0.641564151820134 1103 60-61 -0.7001391464824067 1103 62-63 -0.8372147879572367 1103 64-65 -0.7872447882005957 1103 66-67 -0.7275308954786155 1103 68-69 -0.7928088213307163 1103 70-71 -0.7553585207531697 1103 72-73 -0.8427303847770062 1103 74-75 -0.8024292275433815 1103 76-77 -0.7434626924148375 1103 78-79 -0.8039041057338494 1103 80-81 -0.8455385762050831 1103 82-83 -0.917249015436532 1103 84-85 -0.8525088263086111 1103 86-87 -0.9832340476571062 1103 88-89 -1.0958338022500413 1103 90-91 -1.0472850470742365 1103 92-93 -1.075542907056441 1103 94-95 -1.1786751801900692 1103 96-97 -1.2423572723145888 1103 98-99 -1.3533857973030479 1103 100 -1.3196528498820683 1104 1 -0.4514574349814602 1104 2 -0.45361035957274964 1104 3 -0.47874778834120946 1104 4 -0.3566080080761722 1104 5 -0.2797156730940955 1104 6 -0.37628707660503125 1104 7 -0.2862624324650298 1104 8 -0.3232323275645328 1104 9 -0.43387052832157025 1104 10-11 -0.4280789009853265 1104 12-13 -0.4454212607202308 1104 14-15 -0.5885991351027471 1104 16-17 -0.5477477751498938 1104 18-19 -0.6482083422702729 1104 20-21 -0.6106119574276008 1104 22-23 -0.6177551037324989 1104 24-25 -0.670325131719288 1104 26-27 -0.6929412264542876 1104 28-29 -0.663855907070527 1104 30-31 -0.7028074206717108 1104 32-33 -0.8377263780076234 1104 34-35 -0.7263827629962378 1104 36-37 -0.7604014702256876 1104 38-39 -0.7279272636503578 1104 40-41 -0.7563706685423313 1104 42-43 -0.7712418486878789 1104 44-45 -0.7887416840334041 1104 46-47 -0.8442782039013679 1104 48-49 -0.7954612247400377 1104 50-51 -0.7371183738095795 1104 52-53 -0.6794259881420857 1104 54-55 -0.7600706123781009 1104 56-57 -0.703346182218084 1104 58-59 -0.7322092910151099 1104 60-61 -0.7210381269002184 1104 62-63 -0.6912301287511937 1104 64-65 -0.7332921981055875 1104 66-67 -0.7607569700510552 1104 68-69 -0.6512317389479421 1104 70-71 -0.7108804794376269 1104 72-73 -0.6897987241136789 1104 74-75 -0.7501624643325826 1104 76-77 -0.7616011799197224 1104 78-79 -0.7513291256220853 1104 80-81 -0.766440121911323 1104 82-83 -0.8121897367091648 1104 84-85 -0.7572177072000805 1104 86-87 -0.806635004243411 1104 88-89 -0.7808622700968506 1104 90-91 -0.8176084326601831 1104 92-93 -0.8517361893332414 1104 94-95 -0.8250695077863135 1104 96-97 -0.8759678422309847 1104 98-99 -0.9003471591026297 1104 100 -0.9231763170510412 1105 1 -0.313670776635508 1105 2 -0.42324796309646473 1105 3 -0.4315421263686332 1105 4 -0.27899484368821703 1105 5 -0.23663775676358512 1105 6 -0.25953820978193676 1105 7 -0.23337049793656206 1105 8 -0.2570222558275148 1105 9 -0.34972605156389847 1105 10-11 -0.3588522221049999 1105 12-13 -0.45569564438974197 1105 14-15 -0.5199386901272689 1105 16-17 -0.5619314092342549 1105 18-19 -0.5663890867130519 1105 20-21 -0.5251215565964458 1105 22-23 -0.5791503687334014 1105 24-25 -0.52056833732032 1105 26-27 -0.5133897002231791 1105 28-29 -0.5297510241405234 1105 30-31 -0.5509525223863605 1105 32-33 -0.5974924413382183 1105 34-35 -0.6181014945589993 1105 36-37 -0.6703816311426891 1105 38-39 -0.6550310082891997 1105 40-41 -0.7083195965207452 1105 42-43 -0.7246193824110918 1105 44-45 -0.7040113760075357 1105 46-47 -0.6930431734192126 1105 48-49 -0.7423781610111178 1105 50-51 -0.6678547341639103 1105 52-53 -0.553266923014256 1105 54-55 -0.5498836694706455 1105 56-57 -0.5551183586710096 1105 58-59 -0.4847518465358931 1105 60-61 -0.5247551148184755 1105 62-63 -0.48526760679153824 1105 64-65 -0.47800494493603196 1105 66-67 -0.5019824529839312 1105 68-69 -0.49379700758066747 1105 70-71 -0.46558416040104333 1105 72-73 -0.5672687417792872 1105 74-75 -0.5279748629336396 1105 76-77 -0.5249488982908872 1105 78-79 -0.43888115778269565 1105 80-81 -0.5147478585236627 1105 82-83 -0.4849339622139617 1105 84-85 -0.5047479374037742 1105 86-87 -0.4880802582715482 1105 88-89 -0.5340362083621599 1105 90-91 -0.6033731564171809 1105 92-93 -0.5826777754352719 1105 94-95 -0.7070619982624216 1105 96-97 -0.7408667317589064 1105 98-99 -0.6835483870886065 1105 100 -0.8103733664750266 1106 1 -0.42008595357162193 1106 2 -0.42596571768968516 1106 3 -0.43648674999935366 1106 4 -0.3286685173653936 1106 5 -0.303465674692859 1106 6 -0.342483073557581 1106 7 -0.3453440359220181 1106 8 -0.37855516646644816 1106 9 -0.4462193324032384 1106 10-11 -0.4299543459262978 1106 12-13 -0.44162368079788195 1106 14-15 -0.569115870902273 1106 16-17 -0.6139354936642363 1106 18-19 -0.6197784587986277 1106 20-21 -0.611397681778783 1106 22-23 -0.5987022694156892 1106 24-25 -0.6027236272454317 1106 26-27 -0.634453402191113 1106 28-29 -0.650192377407798 1106 30-31 -0.6531529106696112 1106 32-33 -0.6906288696670515 1106 34-35 -0.745056490736836 1106 36-37 -0.692650121255312 1106 38-39 -0.7571787275683874 1106 40-41 -0.7375949211512136 1106 42-43 -0.7422909954005874 1106 44-45 -0.6733793466237756 1106 46-47 -0.7249699718660452 1106 48-49 -0.7734267482674966 1106 50-51 -0.7175310962117223 1106 52-53 -0.5581680472301684 1106 54-55 -0.5913813647602311 1106 56-57 -0.5601218594393416 1106 58-59 -0.5311345661603255 1106 60-61 -0.5365122031628147 1106 62-63 -0.519500517489945 1106 64-65 -0.5477306169203757 1106 66-67 -0.6392731965548393 1106 68-69 -0.6006362431255354 1106 70-71 -0.6142285910747134 1106 72-73 -0.5920128389335773 1106 74-75 -0.589265926778701 1106 76-77 -0.567937055393152 1106 78-79 -0.5538669634891349 1106 80-81 -0.6047617534553176 1106 82-83 -0.527618460873434 1106 84-85 -0.5496874996154197 1106 86-87 -0.6161156517451758 1106 88-89 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0.5118659366921889 2311 9 0.599830897600107 2311 10-11 0.5889016762692307 2311 12-13 0.6424821525646465 2311 14-15 0.8026435948589992 2311 16-17 0.7696277235698474 2311 18-19 0.6790858416600187 2311 20-21 0.748332178446617 2311 22-23 0.8147401662614158 2311 24-25 0.8317902708570344 2311 26-27 0.8946239447429107 2311 28-29 0.9075447054294656 2311 30-31 0.879563219948011 2311 32-33 0.9246637069518471 2311 34-35 0.9070515224906615 2311 36-37 0.948334858075853 2311 38-39 1.0049748314352556 2311 40-41 0.9894572982093663 2311 42-43 1.058180536919096 2311 44-45 1.0639112385575302 2311 46-47 0.9930360905188707 2311 48-49 0.9659975108060124 2311 50-51 0.9545806690400411 2311 52-53 0.8576168006656815 2311 54-55 0.7801156962055273 2311 56-57 0.7867507879245323 2311 58-59 0.817009214546907 2311 60-61 0.8186578685798409 2311 62-63 0.7872688493690418 2311 64-65 0.8477106576737441 2311 66-67 0.8317752041699507 2311 68-69 0.8074986967239681 2311 70-71 0.798090372435702 2311 72-73 0.755312372592094 2311 74-75 0.7796896488817424 2311 76-77 0.8640939162163974 2311 78-79 0.8545819373973913 2311 80-81 0.886316523312626 2311 82-83 0.8810304537435023 2311 84-85 0.9164266169274491 2311 86-87 0.9323535957157532 2311 88-89 0.915457268568197 2311 90-91 0.9303676060241237 2311 92-93 0.9995533177121096 2311 94-95 1.0168273843492095 2311 96-97 1.0212629779697444 2311 98-99 1.0178588185332096 2311 100 1.0790905904370192 2312 1 0.49432984638778166 2312 2 0.4724383781175554 2312 3 0.5024123275189858 2312 4 0.4436206279919972 2312 5 0.4349242071687769 2312 6 0.43313847593964994 2312 7 0.5013841299183852 2312 8 0.4618798288589474 2312 9 0.6124450679745621 2312 10-11 0.5921826586110868 2312 12-13 0.6114526630593602 2312 14-15 0.7560684364800849 2312 16-17 0.7282387124990422 2312 18-19 0.7178553322333414 2312 20-21 0.7610220599506121 2312 22-23 0.7809056033996171 2312 24-25 0.8052705222241698 2312 26-27 0.8221385330633026 2312 28-29 0.8063880444872638 2312 30-31 0.9178559312931185 2312 32-33 0.848387086283509 2312 34-35 0.86377213651582 2312 36-37 0.8240415745826652 2312 38-39 0.9268407464962038 2312 40-41 0.9085120563468365 2312 42-43 0.9031772284509074 2312 44-45 0.8441302188277646 2312 46-47 0.901527515397774 2312 48-49 0.920479689378837 2312 50-51 0.9203320088358709 2312 52-53 0.7788141292770803 2312 54-55 0.7446423196269265 2312 56-57 0.7267278559676527 2312 58-59 0.7436176157154009 2312 60-61 0.7795384631089561 2312 62-63 0.7403402341227547 2312 64-65 0.8139861642685489 2312 66-67 0.8270652383064103 2312 68-69 0.7854938982210626 2312 70-71 0.8221543566928631 2312 72-73 0.7654024441929081 2312 74-75 0.7836562809398515 2312 76-77 0.8054924039880476 2312 78-79 0.7915114393154283 2312 80-81 0.8575593314669625 2312 82-83 0.8502885063081571 2312 84-85 0.8308223834740467 2312 86-87 0.8804180096614296 2312 88-89 0.8970180990895997 2312 90-91 0.9654163935472653 2312 92-93 0.939105280247226 2312 94-95 0.9886199338698098 2312 96-97 0.9942004539801168 2312 98-99 1.060742313134476 2312 100 1.1579765367483041 2313 1 0.43977863656249383 2313 2 0.466980731142705 2313 3 0.4906304092873839 2313 4 0.3629027966861855 2313 5 0.34094195653811 2313 6 0.40794594420370345 2313 7 0.41908760089456365 2313 8 0.42981909868573354 2313 9 0.5370280695340028 2313 10-11 0.5389132395307641 2313 12-13 0.5260093980971661 2313 14-15 0.712123569269572 2313 16-17 0.6916727974733661 2313 18-19 0.6364752984299926 2313 20-21 0.6280489258414619 2313 22-23 0.6373435759647421 2313 24-25 0.683847060661904 2313 26-27 0.7181773624399597 2313 28-29 0.7154165994376456 2313 30-31 0.7861822113152002 2313 32-33 0.8002475840844241 2313 34-35 0.8361586009954749 2313 36-37 0.8706451436705294 2313 38-39 0.8658232258272989 2313 40-41 0.9096920002210567 2313 42-43 0.7374388560477882 2313 44-45 0.8863552350465014 2313 46-47 0.9546161424338919 2313 48-49 0.9616263316212468 2313 50-51 0.9200567927930976 2313 52-53 0.7920295877169821 2313 54-55 0.7979143580789838 2313 56-57 0.8166020928876918 2313 58-59 0.8109518423257143 2313 60-61 0.7644623881654766 2313 62-63 0.8310996366780898 2313 64-65 0.8389226464823807 2313 66-67 0.7964735087787744 2313 68-69 0.7833280576456758 2313 70-71 0.7575507103691379 2313 72-73 0.8138544667198175 2313 74-75 0.8125946804388349 2313 76-77 0.7868373888566182 2313 78-79 0.7743666937830369 2313 80-81 0.8609781180441978 2313 82-83 0.8070527291405689 2313 84-85 0.8256347612795629 2313 86-87 0.9220717812639236 2313 88-89 0.8707818941176697 2313 90-91 0.9858264035673514 2313 92-93 0.9587112618476823 2313 94-95 1.010826445137301 2313 96-97 1.0477632664351617 2313 98-99 1.030332990007036 2313 100 1.0981529510389016 2314 1 0.33489021056128365 2314 2 0.2164224146585063 2314 3 0.3099402826126152 2314 4 0.2655735447437948 2314 5 0.2787633002763954 2314 6 0.2840573367864536 2314 7 0.2876226647927993 2314 8 0.3093522202730199 2314 9 0.35391032049661675 2314 10-11 0.43669325863741193 2314 12-13 0.4374516822598551 2314 14-15 0.5235514247470903 2314 16-17 0.5554894306610905 2314 18-19 0.5390977158755135 2314 20-21 0.5609536911928217 2314 22-23 0.6087003642929574 2314 24-25 0.6270095401899383 2314 26-27 0.6793786671941575 2314 28-29 0.6630795440988564 2314 30-31 0.6308739434749313 2314 32-33 0.6442140437362696 2314 34-35 0.6562374323753772 2314 36-37 0.6871849209392593 2314 38-39 0.7035434199393364 2314 40-41 0.6707875964484771 2314 42-43 0.5966800215722188 2314 44-45 0.7381052496945131 2314 46-47 0.5064665547245681 2314 48-49 0.4826225516503939 2314 50-51 0.6373554625027893 2314 52-53 0.6423398201143797 2314 54-55 0.6335424711735556 2314 56-57 0.5554057386921443 2314 58-59 0.603440810883086 2314 60-61 0.40940516534741533 2314 62-63 0.5194769077860357 2314 64-65 0.5870792997042074 2314 66-67 0.5626132307767122 2314 68-69 0.5826046650967669 2314 70-71 0.510765573387495 2314 72-73 0.571998378519659 2314 74-75 0.6412132724811599 2314 76-77 0.629602170895744 2314 78-79 0.6062788329601645 2314 80-81 0.6908445485253445 2314 82-83 0.6096493989812295 2314 84-85 0.6954019737923183 2314 86-87 0.6808648507324833 2314 88-89 0.5558530693646233 2314 90-91 0.5767939369891053 2314 92-93 0.7803739978334541 2314 94-95 0.778812294686066 2314 96-97 0.7832445312339615 2314 98-99 0.7492074100005262 2314 100 0.8254317354824892 2315 1 0.26457266145017755 2315 2 0.247360141044215 2315 3 0.28226629854731655 2315 4 0.1665779935120355 2315 5 0.16469391385724919 2315 6 0.23186308552671875 2315 7 0.19195744595145925 2315 8 0.22136084078984908 2315 9 0.0971373527556807 2315 10-11 0.25391188282335264 2315 12-13 0.2286666168477396 2315 14-15 0.3704129214916989 2315 16-17 0.20174829114541382 2315 18-19 0.3918475805495518 2315 20-21 0.24464722547054407 2315 22-23 0.219110441995106 2315 24-25 0.34419526132057143 2315 26-27 0.4445139525595607 2315 28-29 0.30302600524876055 2315 30-31 0.3807459225934835 2315 32-33 0.4029957370383954 2315 34-35 0.3424855239532292 2315 36-37 0.47907404512613994 2315 38-39 0.5307268460520405 2315 40-41 0.5476328641487669 2315 42-43 0.5663519127137846 2315 44-45 0.5653933405857074 2315 46-47 0.6035595247808203 2315 48-49 0.49816085567475454 2315 50-51 0.40128101793415283 2315 52-53 0.21318049557876861 2315 54-55 0.36564272957339483 2315 56-57 0.32077556890977377 2315 58-59 0.3748160839170893 2315 60-61 0.3885149439582065 2315 62-63 0.4110683241617892 2315 64-65 0.43030290330284515 2315 66-67 0.3659931547971027 2315 68-69 0.4206371327292402 2315 70-71 0.3608885969150464 2315 72-73 0.4011623866704852 2315 74-75 0.09470951686628126 2315 76-77 0.3181177488664133 2315 78-79 0.40974119666471154 2315 80-81 0.38739161247496057 2315 82-83 0.3981456676907875 2315 84-85 0.45179395817609347 2315 86-87 0.3985953384398293 2315 88-89 0.31187715058356247 2315 90-91 0.4798553418648197 2315 92-93 0.6514238688380409 2315 94-95 0.5707238647463662 2315 96-97 0.5002925714299451 2315 98-99 0.6729649365918533 2315 100 0.6606275792333705 2316 1 0.13422970219028585 2316 2 0.129989748964789 2316 3 0.1218582063163467 2316 4 0.010045775208290308 2316 5 6.221648642679156E-4 2316 6 -0.1255991139057926 2316 7 -0.06923010568061727 2316 8 -0.04363837609444943 2316 9 -0.00589993256028265 2316 10-11 0.0010622689724542056 2316 12-13 -0.0014050565967167472 2316 14-15 -0.12256025583015173 2316 16-17 0.03417333137802814 2316 18-19 0.08414483325550748 2316 20-21 0.19654476138504862 2316 22-23 0.12666111949807402 2316 24-25 0.10231419165340583 2316 26-27 0.0840189454615583 2316 28-29 0.08044366208935116 2316 30-31 -0.5598301523094804 2316 32-33 -0.39664600928918503 2316 34-35 -0.45332669194021946 2316 36-37 -0.19969650939017924 2316 38-39 -0.0631632049568367 2316 40-41 0.1362890640898371 2316 42-43 0.1309910099225533 2316 44-45 4.7167448793317135E-4 2316 46-47 -0.08129577822212752 2316 48-49 -0.050711688591349 2316 50-51 0.07164817316055405 2316 52-53 -0.28630306818891427 2316 54-55 -0.16181969998687862 2316 56-57 -0.2026893180214131 2316 58-59 -0.4605892017392179 2316 60-61 -0.026547001667594827 2316 62-63 1.161727964529291E-4 2316 64-65 -0.3257173639890425 2316 66-67 -0.5426364434338851 2316 68-69 -0.6366337767818777 2316 70-71 -0.4437876106671652 2316 72-73 -0.6469159404944378 2316 74-75 -0.4646142496621337 2316 76-77 -0.15485505449781556 2316 78-79 -0.08780748084626566 2316 80-81 -0.18865894159927166 2316 82-83 0.005981590075048615 2316 84-85 0.016662751662391173 2316 86-87 0.11658358572392302 2316 88-89 0.1598584342317082 2316 90-91 -0.4129876391587999 2316 92-93 -0.48641217920809154 2316 94-95 -0.1383629383118823 2316 96-97 0.12807619198014208 2316 98-99 0.2482275225935915 2316 100 0.11414460145878991 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 292.0 1 701.0 2 422.0 3 378.0 4 375.0 5 372.0 6 417.0 7 902.0 8 2201.0 9 4367.0 10 7437.0 11 12235.0 12 18108.0 13 23685.0 14 27378.0 15 31477.0 16 36457.0 17 43562.0 18 52726.0 19 63589.0 20 78440.0 21 97321.0 22 107962.0 23 108643.0 24 106094.0 25 108537.0 26 118254.0 27 134896.0 28 157813.0 29 187216.0 30 227895.0 31 281550.0 32 356503.0 33 465356.0 34 642926.0 35 895893.0 36 1324426.0 37 1883973.0 38 1726782.0 39 420698.0 40 254.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.10171037361802 20.23629490184309 13.824848705129932 22.83714601940896 2 15.372331127327875 20.036456948424775 41.24046362224782 23.35074830199952 3 18.681630099544403 26.470105316755856 29.29351598240908 25.55474860129066 4 12.405031297764927 16.80308651421111 38.93056993161771 31.861312256406244 5 13.126891035364924 38.19256992218569 34.67142107464106 14.009117967808326 6 30.284793707023898 37.90183689690698 17.898367591189484 13.915001804879646 7 27.52980677306664 32.447115692672035 22.406235342664477 17.616842191596845 8 26.054642005584327 35.45503686747954 19.91065324435563 18.57966788258051 9 26.51150384801043 16.03158118852509 21.035086462221926 36.42182850124255 10-11 25.071475232708718 26.54046239589583 27.630385693830906 20.75767667756455 12-13 25.581278772037685 24.22973909081194 28.077756868320908 22.111225268829468 14-15 23.172234554911995 25.494153840350393 25.777856388993904 25.55575521574371 16-17 21.8527508521899 28.47695632580574 26.834212415790766 22.836080406213597 18-19 21.612249512054692 27.655682602924436 29.30541672981846 21.42665115520241 20-21 22.66389798202238 26.796056264759876 29.086572434811508 21.453473318406235 22-23 22.863105644266028 26.492685743484508 28.777327219631104 21.866881392618364 24-25 22.367900306231263 26.80682060965591 29.137894410483405 21.687384673629413 26-27 22.135451245592396 27.001469021708647 28.841447677982895 22.021632054716058 28-29 21.787008762367833 26.897723572434508 29.228869799727853 22.086397865469806 30-31 22.321451044542652 26.827978478156815 29.00365127412689 21.84691920317364 32-33 22.391897979323492 27.16312199246729 28.535989103425397 21.908990924783822 34-35 22.428740053728205 27.0390398616704 28.918194302915563 21.614025781685832 36-37 22.39673464952354 26.772639516409917 28.689638699098396 22.140987134968142 38-39 22.333499008002413 26.981724320631333 28.920825346912093 21.763951324454155 40-41 22.15552948544199 26.492953174480625 29.348117630485053 22.003399709592333 42-43 22.109627754877977 26.55229360046357 28.88251101930684 22.455567625351616 44-45 22.099928579158988 27.136069390850615 28.671020205520374 22.09298182447002 46-47 22.225208895983535 26.974725972539986 28.28403578498861 22.51602934648787 48-49 22.476187155957657 26.950540957476534 28.246634364219236 22.32663752234657 50-51 22.457587583426825 26.966486137105537 27.955960520684688 22.61996575878295 52-53 22.128642697091912 27.335583616994313 27.950559522046802 22.585214163866972 54-55 22.19302716768538 27.240689535286855 27.899152047594544 22.66713124943322 56-57 22.43950216020229 27.162700208009987 27.61949167639644 22.77830595539128 58-59 22.105249343482186 27.127542702494207 28.11261932178606 22.654588632237548 60-61 22.2545345757476 27.566352290193162 27.67929857552758 22.499814558531657 62-63 22.303180235672546 27.251803317448925 27.78616858605445 22.65884786082408 64-65 21.9621863403863 27.044211323243296 27.907554488515007 23.0860478478554 66-67 22.227263168892183 26.977377974316596 27.710256433127984 23.085102423663237 68-69 21.9549847642026 27.13188504266982 27.850490115533876 23.062640077593702 70-71 22.112896930707908 26.808605667788544 27.953014561946322 23.125482839557232 72-73 22.377079546122193 27.09830508961188 27.683437221134028 22.841178143131895 74-75 22.35012361177056 27.380496745739308 27.564373932244575 22.705005710245562 76-77 22.258591112151883 27.20891024018983 27.552445021516487 22.9800536261418 78-79 22.201284669664723 27.118959500421735 27.561556746310146 23.1181990836034 80-81 22.11414134060397 27.590841089488098 27.51044094470426 22.784576625203666 82-83 22.643872717739434 27.084949440081168 27.914087816549305 22.357090025630093 84-85 22.515667502082216 27.29608972000028 27.370847500587264 22.81739527733024 86-87 22.09492146697512 27.625886740703244 27.668186274823935 22.61100551749771 88-89 22.47409238096615 26.99334830219128 27.6292684137307 22.903290903111866 90-91 22.47911513783786 27.021139717239816 27.73053463267977 22.769210512242555 92-93 22.60645892380326 27.238665861640637 27.496935645347577 22.657939569208523 94-95 22.122627161421633 26.942210513642163 27.816460350118362 23.118701974817846 96-97 22.466814770740697 26.927919299790354 27.55952543935132 23.045740490117627 98-99 22.194841499116215 27.191122922854788 27.445872190613457 23.168163387415547 100 22.688079664741885 26.83027357872318 26.95201529482273 23.529631461712206 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2850.0 1 2020.0 2 1723.0 3 2258.5 4 1868.5 5 1683.0 6 2571.0 7 4766.5 8 8060.5 9 9931.5 10 8655.5 11 7031.5 12 7164.5 13 8107.5 14 9163.0 15 10139.0 16 11150.5 17 12089.5 18 12848.0 19 13777.0 20 14979.5 21 16315.5 22 18288.5 23 21090.0 24 23868.0 25 28027.5 26 35117.0 27 43281.5 28 52124.0 29 66659.0 30 88924.5 31 111087.0 32 141700.0 33 178892.0 34 212994.5 35 245140.5 36 283286.5 37 327834.5 38 344006.0 39 337652.0 40 322913.0 41 305675.0 42 299737.0 43 307357.0 44 322056.0 45 347418.5 46 367779.0 47 373135.0 48 381795.0 49 382518.0 50 373434.5 51 367662.0 52 358337.5 53 345149.0 54 331160.0 55 303268.0 56 273412.5 57 236887.5 58 201372.0 59 179116.5 60 150164.0 61 119762.5 62 95629.0 63 75742.5 64 56472.0 65 38293.5 66 27073.0 67 20531.5 68 15970.0 69 12217.5 70 9414.5 71 7311.0 72 5597.0 73 4477.0 74 3566.0 75 2610.0 76 1853.5 77 1215.0 78 769.5 79 513.5 80 372.0 81 256.5 82 174.5 83 133.5 84 108.5 85 78.5 86 65.0 87 54.0 88 43.5 89 37.5 90 30.0 91 18.0 92 13.0 93 14.5 94 14.0 95 13.0 96 9.0 97 4.5 98 2.5 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024583663515127765 2 0.10838741517278298 3 0.017021035889381916 4 0.017892070236520667 5 0.02001329505837621 6 0.045088836793064684 7 0.04547824038355024 8 0.03801808738687954 9 0.03291485085893722 10-11 0.036486091682206095 12-13 0.035768769278680064 14-15 0.031244514405012323 16-17 0.03735200229789108 18-19 0.036660298551633844 20-21 0.0477890432691948 22-23 0.04800936372170637 24-25 0.06732070757091782 26-27 0.05906637619891473 28-29 0.05108360259396079 30-31 0.049131460910079236 32-33 0.04360295467147505 34-35 0.0452784148568537 36-37 0.04068755147428712 38-39 0.045672942178793016 40-41 0.054588234908330806 42-43 0.05654037659221236 44-45 0.058999767690015886 46-47 0.06078794996737721 48-49 0.0604395362285217 50-51 0.05487516386974122 52-53 0.06415936526394954 54-55 0.050812044826911644 56-57 0.05508523685934527 58-59 0.05287690860277585 60-61 0.04879329463413124 62-63 0.05581280672577882 64-65 0.0539528922080649 66-67 0.05179067753457929 68-69 0.057995516325079446 70-71 0.05801601125089448 72-73 0.059886173231515905 74-75 0.051155334834313386 76-77 0.0518624097749319 78-79 0.06114661116914022 80-81 0.06192541835011134 82-83 0.05262072203008798 84-85 0.05822608424049853 86-87 0.055853796577408875 88-89 0.06421060257848711 90-91 0.07063063809004506 92-93 0.0745554163836232 94-95 0.07828036915050479 96-97 0.07441707563437175 98-99 0.08342971926153094 100 0.1277858624567083 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9758513.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.78332485254096 #Duplication Level Percentage of deduplicated Percentage of total 1 69.25949026808378 32.40189232333163 2 15.716609203524424 14.705504678978363 3 6.199208964351084 8.700588204240624 4 2.98874438581009 5.5929359801024585 5 1.764461680884313 4.127369200333564 6 1.0525248194056547 2.954436632497005 7 0.7091193649700073 2.3222473127433583 8 0.4599805857568584 1.7215536935460147 9 0.337242714394516 1.4199601915504199 >10 1.3701209057229506 10.742279388011298 >50 0.07206028165998947 2.3309308484935816 >100 0.05859693175897261 5.7185983734637285 >500 0.008146731283442971 2.660749467650234 >1k 0.003512125021416428 2.8610242390539593 >5k 7.241494888468338E-5 0.20945148171321787 >10k+ 1.0862242332702508E-4 1.530477984290551 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 40427 0.4142741829620967 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31502 0.32281557651252807 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18423 0.18878900914514332 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.02474629075147E-5 2 0.0 0.0 0.0 0.0 1.02474629075147E-5 3 0.0 0.0 0.0 0.0 1.02474629075147E-5 4 0.0 0.0 0.0 0.0 1.02474629075147E-5 5 0.0 0.0 0.0 0.0 1.02474629075147E-5 6 0.0 0.0 0.0 0.0 3.07423887225441E-5 7 0.0 0.0 0.0 0.0 3.07423887225441E-5 8 0.0 0.0 0.0 0.0 4.09898516300588E-5 9 0.0 0.0 0.0 0.0 4.09898516300588E-5 10-11 0.0 0.0 0.0 0.0 4.09898516300588E-5 12-13 0.0 0.0 0.0 0.0 4.09898516300588E-5 14-15 0.0 0.0 0.0 0.0 9.22271661676323E-5 16-17 0.0 0.0 0.0 0.0 1.229695548901764E-4 18-19 0.0 0.0 0.0 1.02474629075147E-5 1.434644807052058E-4 20-21 0.0 0.0 0.0 1.0759836052890435E-4 1.434644807052058E-4 22-23 0.0 0.0 0.0 3.1254761867919835E-4 1.6908313797399255E-4 24-25 0.0 0.0 0.0 8.095495696936613E-4 1.947017952427793E-4 26-27 0.0 0.0 0.0 0.001829172128991374 2.1007298960405135E-4 28-29 0.0 0.0 0.0 0.004554997262390285 2.151967210578087E-4 30-31 0.0 0.0 0.0 0.013951920748581264 2.2032045251156606E-4 32-33 0.0 0.0 0.0 0.03607106943445174 2.356916468728381E-4 34-35 0.0 0.0 0.0 0.06962638672510862 2.459391097803528E-4 36-37 0.0 0.0 0.0 0.12571587494939035 2.510628412341102E-4 38-39 0.0 0.0 0.0 0.21003712348387504 2.613103041416248E-4 40-41 0.0 0.0 0.0 0.2988108946516749 2.664340355953822E-4 42-43 0.0 0.0 0.0 0.3868980858046713 2.664340355953822E-4 44-45 0.0 0.0 0.0 0.4948294888780699 2.8180522995665424E-4 46-47 0.0 0.0 0.0 0.6236400976255296 3.0742388722544104E-4 48-49 0.0 0.0 0.0 0.7651575603783076 3.381662759479851E-4 50-51 0.0 0.0 0.0 0.9103692335092447 3.381662759479851E-4 52-53 0.0 0.0 0.0 1.051963552233829 3.689086646705292E-4 54-55 0.0 0.0 0.0 1.2052502261358877 4.09898516300588E-4 56-57 0.0 0.0 0.0 1.3850778289684094 4.09898516300588E-4 58-59 0.0 0.0 0.0 1.5901295617477786 4.09898516300588E-4 60-61 0.0 0.0 0.0 1.8053826438515785 4.09898516300588E-4 62-63 0.0 0.0 0.0 2.0211890889523847 4.09898516300588E-4 64-65 0.0 0.0 0.0 2.2409971683185748 4.150222477543454E-4 66-67 0.0 0.0 0.0 2.4886014908213987 4.2526971066186E-4 68-69 0.0 0.0 0.0 2.759662255919524 4.303934421156174E-4 70-71 0.0 0.0 0.0 3.0528011798518895 4.508883679306468E-4 72-73 0.0 0.0 0.0 3.351171433598541 4.8163075665319093E-4 74-75 0.0 0.0 0.0 3.6412514898530137 4.8163075665319093E-4 76-77 0.0 0.0 0.0 3.9597887506016543 4.918782195607056E-4 78-79 0.0 0.0 0.0 4.305251220139789 4.918782195607056E-4 80-81 0.0 0.0 0.0 4.675041166620365 5.021256824682204E-4 82-83 0.0 0.0 0.0 5.04840235392421 5.636104599133085E-4 84-85 0.0 0.0 0.0 5.413883242252176 5.636104599133085E-4 86-87 0.0 0.0 0.0 5.795109357337537 5.636104599133085E-4 88 0.0 0.0 0.0 6.095979992033622 5.84105385728338E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 17760 0.0 52.357956 1 GTATCAA 33240 0.0 43.002 1 CAACGCA 40430 0.0 34.251087 5 ATCAACG 40800 0.0 33.964554 3 TATCAAC 42965 0.0 32.698696 2 GTACTAG 4530 0.0 31.979486 1 AACGCAG 43970 0.0 31.846699 6 TCAACGC 43720 0.0 31.80326 4 ACGCAGA 48485 0.0 28.611036 7 CGCAGAG 49630 0.0 27.835768 8 GTACATG 44620 0.0 26.015642 1 GCAGAGT 53400 0.0 25.404991 9 GCATCGT 4965 0.0 25.174597 8 TACATGG 47065 0.0 24.453777 2 TAGGCAT 6695 0.0 24.073626 5 GTGGTAT 8570 0.0 24.03877 1 ACATGGG 46440 0.0 23.82923 3 TAGTACT 6350 0.0 23.014072 4 GAGTACT 29255 0.0 22.23843 12-13 TGGTATC 8925 0.0 21.922756 2 >>END_MODULE