FastQCFastQC Report
Mon 6 Jun 2016
ERR1211294_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211294_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9758513
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350300.35896862565023996No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289280.29643860698858526No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176580.18094970002089455No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA183950.053.090061
GTATCAA340000.041.1042481
CAACGCA414750.032.8235055
ATCAACG415650.032.7923433
TATCAAC443600.031.3590412
AACGCAG450900.030.618356
GTACTAG46200.030.473351
TCAACGC448950.030.4335044
ACGCAGA503450.027.0402417
CGCAGAG512800.026.4923258
GTACATG446750.024.7906761
TAGGCAT64650.024.3806215
GCAGAGT555450.024.060579
TACATGG463250.023.6686172
ACATGGG460050.023.1716733
GTGGTAT87600.022.928761
CTAGGCA68100.022.3920974
TAGTACT62300.022.2926754
CGTTAAC14900.022.0471611
CTGTGCG61500.022.0435289
Produced by FastQC (version 0.11.5)