FastQCFastQC Report
Mon 6 Jun 2016
ERR1211293_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211293_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5639865
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204390.3624022915442125No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160250.2841380068494547No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87530.1551987503246975No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98050.051.5679051
GTATCAA185400.042.0115971
ATCAACG221700.034.2080733
CAACGCA221950.034.168935
TATCAAC230000.033.3522762
TCAACGC233600.032.545914
AACGCAG239600.031.964826
ACGCAGA264450.028.6253227
CGCAGAG272300.027.6953328
GTACTAG23100.027.08061
GTGGTAT46850.025.8013341
GCAGAGT295400.025.1021189
GTACATG244100.024.9528751
TACATGG250600.024.060592
TGGTATC48400.023.8078422
ACATGGG246800.023.2657363
GAGTACT163200.022.17026712-13
CATGGGG167350.021.5919254
CAGAGTA295200.020.74926610-11
GTACTTT173200.020.59426314-15
Produced by FastQC (version 0.11.5)