##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211293_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5639865 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.36382502063436 31.0 31.0 34.0 28.0 34.0 2 31.381422782282907 31.0 31.0 34.0 28.0 34.0 3 31.42669833409133 31.0 31.0 34.0 28.0 34.0 4 35.03870943719397 37.0 35.0 37.0 32.0 37.0 5 35.08187004476171 37.0 35.0 37.0 32.0 37.0 6 35.148970409752714 37.0 35.0 37.0 32.0 37.0 7 35.08311386886034 37.0 35.0 37.0 32.0 37.0 8 35.08463003990344 37.0 35.0 37.0 32.0 37.0 9 36.63584465230994 39.0 37.0 39.0 32.0 39.0 10-11 36.572843587568144 39.0 37.0 39.0 32.0 39.0 12-13 36.48762399454597 39.0 37.0 39.0 32.0 39.0 14-15 37.60829080128691 40.0 37.0 41.0 32.0 41.0 16-17 37.60718315066052 40.0 37.0 41.0 32.0 41.0 18-19 37.61289108870514 40.0 37.0 41.0 32.0 41.0 20-21 37.5849246391536 40.0 37.0 41.0 32.0 41.0 22-23 37.46384745024925 40.0 37.0 41.0 31.5 41.0 24-25 37.3494315910044 40.0 37.0 41.0 31.0 41.0 26-27 37.20320087448901 40.0 36.5 41.0 31.0 41.0 28-29 37.06795543864968 40.0 36.0 41.0 30.0 41.0 30-31 36.886462707883965 39.0 36.0 41.0 30.0 41.0 32-33 36.76554660439567 39.0 36.0 41.0 30.0 41.0 34-35 36.62155379605717 39.0 35.0 41.0 30.0 41.0 36-37 36.52851025689445 39.0 35.0 41.0 30.0 41.0 38-39 36.37796746907949 39.0 35.0 41.0 29.0 41.0 40-41 36.21467517396249 39.0 35.0 40.0 28.5 41.0 42-43 36.06659662952926 39.0 35.0 40.0 28.0 41.0 44-45 35.91850655999745 39.0 35.0 40.0 27.0 41.0 46-47 35.74130089993289 38.5 35.0 40.0 26.5 41.0 48-49 35.49633501865736 38.0 34.0 40.0 26.0 41.0 50-51 34.82830599668609 37.5 33.5 39.5 25.0 40.5 52-53 35.068137446552356 38.0 34.0 40.0 26.0 40.5 54-55 35.424062100777235 38.0 34.0 40.0 26.0 41.0 56-57 35.32015420581875 38.0 34.0 40.0 26.0 41.0 58-59 35.095690233720134 38.0 34.0 40.0 26.0 41.0 60-61 34.83314290324325 37.5 34.0 40.0 26.0 41.0 62-63 34.56235867347888 37.0 33.0 40.0 25.0 41.0 64-65 34.28470504169869 36.5 33.0 40.0 25.0 41.0 66-67 34.06300345841611 36.0 33.0 39.0 24.5 41.0 68-69 33.728708931862734 35.5 32.5 39.0 23.5 41.0 70-71 33.39618510372146 35.0 32.0 39.0 23.5 40.5 72-73 33.041255420120876 35.0 32.0 38.0 23.0 40.0 74-75 32.67403288908511 35.0 31.5 37.0 22.0 39.5 76-77 32.32000136528091 35.0 31.0 37.0 22.0 39.0 78-79 31.957095604238752 35.0 31.0 36.0 20.5 39.0 80-81 31.626885749924867 35.0 31.0 36.0 20.0 37.5 82-83 31.313738449413236 34.0 30.0 35.0 20.0 37.0 84-85 31.01185099288724 34.0 30.0 35.0 19.5 37.0 86-87 30.760211724926037 34.0 30.0 35.0 19.0 36.0 88-89 30.50368803153976 34.0 29.5 35.0 18.0 36.0 90-91 30.24355467728394 34.0 29.0 35.0 18.0 36.0 92-93 30.00724574081117 34.0 29.0 35.0 17.0 35.0 94-95 29.796559137497084 34.0 29.0 35.0 17.0 35.0 96-97 29.60028537917131 34.0 29.0 35.0 15.0 35.0 98-99 29.361912829473752 34.0 27.5 35.0 14.0 35.0 100 27.675452869882523 32.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.35304025429731567 1101 2 -0.3393227537870196 1101 3 -0.3611948064444199 1101 4 -0.263862488962495 1101 5 -0.31186802447881945 1101 6 -0.33062484265077074 1101 7 -0.3848433874312178 1101 8 -0.45984003906606574 1101 9 -0.4935778464220988 1101 10-11 -0.4355624353027139 1101 12-13 -0.40710224883932966 1101 14-15 -0.5550643771990877 1101 16-17 -0.5785881487020106 1101 18-19 -0.6072440807825146 1101 20-21 -0.6751308195059877 1101 22-23 -0.6441879349043944 1101 24-25 -0.5254677062176043 1101 26-27 -0.6402800780223288 1101 28-29 -0.6544206047076742 1101 30-31 -0.6997548664830759 1101 32-33 -0.6945978882732433 1101 34-35 -0.6666658031222639 1101 36-37 -0.7735381516773572 1101 38-39 -0.8195413314102211 1101 40-41 -0.7769902568143152 1101 42-43 -0.7604476853788498 1101 44-45 -0.8941150610194626 1101 46-47 -0.7976650594004084 1101 48-49 -0.8244088372325891 1101 50-51 -0.8622312639624212 1101 52-53 -0.6532279389614075 1101 54-55 -0.6209247040394885 1101 56-57 -0.5520878770241566 1101 58-59 -0.5935742560897168 1101 60-61 -0.6660103759435287 1101 62-63 -0.5820849564544588 1101 64-65 -0.6152856578267034 1101 66-67 -0.7495080432092536 1101 68-69 -0.7768844279155971 1101 70-71 -0.7241060648316378 1101 72-73 -0.7355702406663625 1101 74-75 -0.726368642935018 1101 76-77 -0.6560512833057501 1101 78-79 -0.6552628984591422 1101 80-81 -0.7443824968050308 1101 82-83 -0.905939553121442 1101 84-85 -0.8353524102382544 1101 86-87 -0.9406751439241212 1101 88-89 -0.94765491420657 1101 90-91 -0.9415263444482314 1101 92-93 -0.9199317911457356 1101 94-95 -0.9591372486959564 1101 96-97 -1.126969381957256 1101 98-99 -1.197538583093948 1101 100 -1.0183353616544473 1102 1 -0.3425355959584273 1102 2 -0.3996743162870189 1102 3 -0.35252919513228775 1102 4 -0.264302546511054 1102 5 -0.24737621637083862 1102 6 -0.2188601192647539 1102 7 -0.3886824999216927 1102 8 -0.379788955046358 1102 9 -0.41981838393876103 1102 10-11 -0.4280967442438737 1102 12-13 -0.43092206069064076 1102 14-15 -0.5660187266682399 1102 16-17 -0.5979372719344838 1102 18-19 -0.5482838455297099 1102 20-21 -0.5787947979446173 1102 22-23 -0.681104136133925 1102 24-25 -0.7206720980893877 1102 26-27 -0.7195404086438799 1102 28-29 -0.8120346864004446 1102 30-31 -0.7950765537212234 1102 32-33 -0.741224181925638 1102 34-35 -0.7645603633845894 1102 36-37 -0.7455427586867316 1102 38-39 -0.8965330279323069 1102 40-41 -0.8640193829629013 1102 42-43 -0.9241273713910374 1102 44-45 -0.8189609423099284 1102 46-47 -0.9186054717920982 1102 48-49 -0.9645922918721865 1102 50-51 -0.8101676503352522 1102 52-53 -0.6532471689295036 1102 54-55 -0.6341380354287409 1102 56-57 -0.5921913803747074 1102 58-59 -0.6301336056481333 1102 60-61 -0.7113726618474061 1102 62-63 -0.72479034516018 1102 64-65 -0.7766925996592136 1102 66-67 -0.7261750061984387 1102 68-69 -0.7318201879196451 1102 70-71 -0.6790873890734517 1102 72-73 -0.7497581074119779 1102 74-75 -0.802800345676161 1102 76-77 -0.6988111631203111 1102 78-79 -0.7192140357425174 1102 80-81 -0.8422396757947475 1102 82-83 -0.902056791950951 1102 84-85 -0.9827750179855919 1102 86-87 -1.1200322195109145 1102 88-89 -1.0474055941365847 1102 90-91 -1.118089755732985 1102 92-93 -1.1329038366489783 1102 94-95 -1.0448641117339506 1102 96-97 -1.2523230162352483 1102 98-99 -1.0945673112528702 1102 100 -1.1686592210978866 1103 1 -0.4432568500041292 1103 2 -0.4462274222844691 1103 3 -0.427704508980856 1103 4 -0.3374218304214125 1103 5 -0.2815907674202691 1103 6 -0.3405493846629497 1103 7 -0.4088048977983121 1103 8 -0.42493663857420927 1103 9 -0.658981413558223 1103 10-11 -0.6012593202540728 1103 12-13 -0.6285346737864685 1103 14-15 -0.6557283094703124 1103 16-17 -0.6738542599077135 1103 18-19 -0.6925198663957346 1103 20-21 -0.8331327812836733 1103 22-23 -0.8686161276624631 1103 24-25 -0.8740804123180084 1103 26-27 -0.716247412309535 1103 28-29 -0.8979977402328316 1103 30-31 -0.8913562681925882 1103 32-33 -0.8594854409365311 1103 34-35 -0.8979542034934624 1103 36-37 -1.1096653695758079 1103 38-39 -0.9583013531333648 1103 40-41 -0.9019331864765334 1103 42-43 -0.851530580983038 1103 44-45 -0.9343931966866421 1103 46-47 -0.954005191964221 1103 48-49 -0.9568325852470352 1103 50-51 -0.9674078900738792 1103 52-53 -0.9179831101074214 1103 54-55 -0.8194283760034793 1103 56-57 -0.844836987414233 1103 58-59 -0.8739732930868342 1103 60-61 -0.8359520388702535 1103 62-63 -0.8636707501596845 1103 64-65 -0.8578553258508563 1103 66-67 -0.8439071821130213 1103 68-69 -0.8358455486616023 1103 70-71 -0.7826886404833218 1103 72-73 -0.9462002247251391 1103 74-75 -0.7955928240377759 1103 76-77 -0.8088531296390435 1103 78-79 -0.8382844739045403 1103 80-81 -0.8439761104259738 1103 82-83 -0.9757707100928705 1103 84-85 -0.9025363291002435 1103 86-87 -1.0102416536640106 1103 88-89 -1.1648665622858623 1103 90-91 -0.9540079850018195 1103 92-93 -0.9544682828778903 1103 94-95 -1.2706708772547408 1103 96-97 -1.2934978259032555 1103 98-99 -1.1505240743217193 1103 100 -1.1483874080094196 1104 1 -0.3479038219051027 1104 2 -0.3165252267391061 1104 3 -0.3847790019329054 1104 4 -0.3016543263603637 1104 5 -0.24094931818691379 1104 6 -0.1701666300839193 1104 7 -0.25512286168753207 1104 8 -0.2817918892619673 1104 9 -0.29994541253227425 1104 10-11 -0.3492025692052323 1104 12-13 -0.3140713011755736 1104 14-15 -0.45630774086838954 1104 16-17 -0.43785060389932084 1104 18-19 -0.5785138131688115 1104 20-21 -0.520383228924139 1104 22-23 -0.4366856360856204 1104 24-25 -0.5434359661825141 1104 26-27 -0.5836519631744039 1104 28-29 -0.5777503995476678 1104 30-31 -0.6061652926122605 1104 32-33 -0.6736468116107304 1104 34-35 -0.6279566273915478 1104 36-37 -0.6585008525333365 1104 38-39 -0.6291791953395744 1104 40-41 -0.7687375231773572 1104 42-43 -0.7355780572488442 1104 44-45 -0.776426136233809 1104 46-47 -0.8408585690196873 1104 48-49 -0.7390522392253516 1104 50-51 -0.7794823057158524 1104 52-53 -0.6583763106412519 1104 54-55 -0.6067900591922708 1104 56-57 -0.5630069534523798 1104 58-59 -0.6550887773540879 1104 60-61 -0.5377925263663315 1104 62-63 -0.6034111732610299 1104 64-65 -0.6368690752194937 1104 66-67 -0.643493368592182 1104 68-69 -0.5893620578481631 1104 70-71 -0.6110819613783178 1104 72-73 -0.6953385206171561 1104 74-75 -0.7653518151989616 1104 76-77 -0.6733732948339899 1104 78-79 -0.6878438898769836 1104 80-81 -0.7037356427576533 1104 82-83 -0.6376859276252205 1104 84-85 -0.7883390092433196 1104 86-87 -0.869135502692906 1104 88-89 -0.7833222648282394 1104 90-91 -0.6412330794868524 1104 92-93 -0.6573366153390836 1104 94-95 -0.727006149504426 1104 96-97 -0.7609211434087406 1104 98-99 -0.7574872180341679 1104 100 -1.0583016211172058 1105 1 -0.44789420955816084 1105 2 -0.4551741000137923 1105 3 -0.42918709236399266 1105 4 -0.31012454771981623 1105 5 -0.3588702495998888 1105 6 -0.4307290754649813 1105 7 -0.37103216040235054 1105 8 -0.39493111766012134 1105 9 -0.4750379753304941 1105 10-11 -0.561016788328061 1105 12-13 -0.5311299274070862 1105 14-15 -0.6211237376892811 1105 16-17 -0.7317685891865722 1105 18-19 -0.6654232765829917 1105 20-21 -0.7132647878123706 1105 22-23 -0.6540747600081147 1105 24-25 -0.6796161440578246 1105 26-27 -0.7201118662069277 1105 28-29 -0.7792934010264432 1105 30-31 -0.795202095885621 1105 32-33 -0.7650635279509501 1105 34-35 -0.7281946868345415 1105 36-37 -0.8659296581581941 1105 38-39 -0.7813899765244656 1105 40-41 -0.8334937871746035 1105 42-43 -0.8058469509321853 1105 44-45 -0.8860228631124514 1105 46-47 -0.8895432013972382 1105 48-49 -0.935565536207605 1105 50-51 -0.8954397027978089 1105 52-53 -0.6568750041826377 1105 54-55 -0.7085083248201016 1105 56-57 -0.7108089130230795 1105 58-59 -0.6521921786596039 1105 60-61 -0.6315964676044103 1105 62-63 -0.7119753787565983 1105 64-65 -0.5907098452270958 1105 66-67 -0.6812583528636864 1105 68-69 -0.7029523412307057 1105 70-71 -0.6203816139857565 1105 72-73 -0.6931154455921984 1105 74-75 -0.666605354623357 1105 76-77 -0.7122814670286317 1105 78-79 -0.7001302935639053 1105 80-81 -0.7111311421864777 1105 82-83 -0.6366880238788042 1105 84-85 -0.6453457141483696 1105 86-87 -0.7460250395360326 1105 88-89 -0.7273995058971074 1105 90-91 -0.8941462726674771 1105 92-93 -0.8973428150098002 1105 94-95 -0.8897370289931459 1105 96-97 -0.9722008588949933 1105 98-99 -1.0328735215534017 1105 100 -1.0047267072326989 1106 1 -0.4372929354330033 1106 2 -0.4262624005947053 1106 3 -0.41970062439679623 1106 4 -0.3912881541061495 1106 5 -0.30935202233338543 1106 6 -0.3478029074463933 1106 7 -0.31273140802134236 1106 8 -0.35286399173615024 1106 9 -0.4575867348993654 1106 10-11 -0.42389060825512814 1106 12-13 -0.494594940111611 1106 14-15 -0.5961935413999484 1106 16-17 -0.6871984388214969 1106 18-19 -0.7403616930821073 1106 20-21 -0.670358438815235 1106 22-23 -0.7314817415640107 1106 24-25 -0.7218629812824346 1106 26-27 -0.6597561165749397 1106 28-29 -0.7332243870299351 1106 30-31 -0.7954644876310795 1106 32-33 -0.8113107630131182 1106 34-35 -0.6932790330309189 1106 36-37 -0.8479208674643886 1106 38-39 -0.8524145759989707 1106 40-41 -0.7773365078476786 1106 42-43 -0.8202844946687406 1106 44-45 -0.7744799702123686 1106 46-47 -0.824931561418289 1106 48-49 -0.863988464308143 1106 50-51 -0.8110086918098816 1106 52-53 -0.6731024489241761 1106 54-55 -0.7271585604869628 1106 56-57 -0.6540522464660583 1106 58-59 -0.6222465321290613 1106 60-61 -0.6927604949361381 1106 62-63 -0.606020995843032 1106 64-65 -0.7207249367080237 1106 66-67 -0.6923989632683529 1106 68-69 -0.6446899853980526 1106 70-71 -0.7514745869133037 1106 72-73 -0.6499244433146103 1106 74-75 -0.6841541042573311 1106 76-77 -0.6704314002697842 1106 78-79 -0.5926796595296331 1106 80-81 -0.6580210622443872 1106 82-83 -0.6294116663869609 1106 84-85 -0.45018919495167964 1106 86-87 -0.6409071383991822 1106 88-89 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0.7132440446811685 2311 22-23 0.714553545168414 2311 24-25 0.7374535712246555 2311 26-27 0.8053376762578495 2311 28-29 0.8189369425585014 2311 30-31 0.7906369835035392 2311 32-33 0.8008165081280509 2311 34-35 0.8110894769491779 2311 36-37 0.82561560586106 2311 38-39 0.8859092899173575 2311 40-41 0.9101365393457925 2311 42-43 0.8691645876336125 2311 44-45 0.9131219599568965 2311 46-47 0.8907143022280977 2311 48-49 0.8427958145687384 2311 50-51 0.8680148470722884 2311 52-53 0.6774564466988764 2311 54-55 0.7058540539455791 2311 56-57 0.7382941630477191 2311 58-59 0.8055216164551027 2311 60-61 0.7552530837866129 2311 62-63 0.727340026917858 2311 64-65 0.7431667959350676 2311 66-67 0.6726992590703986 2311 68-69 0.6965900882617362 2311 70-71 0.6844858908197509 2311 72-73 0.6439701928816675 2311 74-75 0.7038835424508747 2311 76-77 0.7544405976904756 2311 78-79 0.7076177041590235 2311 80-81 0.7173098304271512 2311 82-83 0.7361755920669211 2311 84-85 0.8206791118940409 2311 86-87 0.7933747010145602 2311 88-89 0.8549681689952955 2311 90-91 0.9077373316788204 2311 92-93 0.8735942941174208 2311 94-95 0.9060900999170798 2311 96-97 0.95461745236938 2311 98-99 0.9747183519797993 2311 100 1.1004421233450508 2312 1 0.5095857319201222 2312 2 0.5106772462129818 2312 3 0.5582062149623503 2312 4 0.4668544181971441 2312 5 0.4343950277143378 2312 6 0.467974462754178 2312 7 0.528283810212379 2312 8 0.5300241117911852 2312 9 0.5366829961874231 2312 10-11 0.6304279994469795 2312 12-13 0.6301297310088856 2312 14-15 0.8410513393806625 2312 16-17 0.7646542230379083 2312 18-19 0.8116142469387455 2312 20-21 0.8199171869725674 2312 22-23 0.8630891677895818 2312 24-25 0.8869792845013009 2312 26-27 0.8946480894830842 2312 28-29 0.9338240108590199 2312 30-31 0.9490190939522805 2312 32-33 0.9114858560278307 2312 34-35 0.9718534033740838 2312 36-37 1.0091419687383478 2312 38-39 1.0537165038105911 2312 40-41 1.046915344462171 2312 42-43 1.0625223722998172 2312 44-45 0.9061669042003189 2312 46-47 0.9938886749941744 2312 48-49 1.0410360352177221 2312 50-51 1.063349022081944 2312 52-53 0.8945021163622826 2312 54-55 0.8221452354703089 2312 56-57 0.8322889158595146 2312 58-59 0.9222578942532849 2312 60-61 0.9855007978331969 2312 62-63 0.8890038312002346 2312 64-65 0.8876280578723339 2312 66-67 0.9298282346335824 2312 68-69 0.952370897943922 2312 70-71 0.81203821473175 2312 72-73 0.9282569137828176 2312 74-75 0.7496472218514398 2312 76-77 0.8180630359136032 2312 78-79 0.882891888942023 2312 80-81 0.9475141195522561 2312 82-83 0.8409467692675037 2312 84-85 0.9097017539978474 2312 86-87 0.950321869112905 2312 88-89 0.9878362976220885 2312 90-91 1.025985251660547 2312 92-93 1.043740608343139 2312 94-95 1.0211003746619483 2312 96-97 1.065931925109389 2312 98-99 1.1392328415468143 2312 100 1.0825613279951725 2313 1 0.45942064525681303 2313 2 0.4915540126618474 2313 3 0.5664463594946696 2313 4 0.45514076686266947 2313 5 0.4496169623823931 2313 6 0.4624214583830195 2313 7 0.4719231083160338 2313 8 0.5244324481883353 2313 9 0.604824290046956 2313 10-11 0.6255058380004641 2313 12-13 0.5935866308788391 2313 14-15 0.7830228739148808 2313 16-17 0.7666815318424867 2313 18-19 0.7152368781919947 2313 20-21 0.7605227420141603 2313 22-23 0.7735704093141464 2313 24-25 0.6824438352081685 2313 26-27 0.7921162693163453 2313 28-29 0.8008773217717575 2313 30-31 0.8357957313133468 2313 32-33 0.8893024554822375 2313 34-35 0.8787486476414585 2313 36-37 0.9242160492214495 2313 38-39 0.8995222887463825 2313 40-41 1.0049578188063961 2313 42-43 0.8424505375513718 2313 44-45 0.9218906658945656 2313 46-47 1.0080940971611412 2313 48-49 1.0338869654120444 2313 50-51 0.8864230469609353 2313 52-53 0.8231166946075703 2313 54-55 0.8211529419113219 2313 56-57 0.7946381436575436 2313 58-59 0.7352529445294564 2313 60-61 0.7813949142001206 2313 62-63 0.7774680650595869 2313 64-65 0.8319046820513947 2313 66-67 0.7832509068229498 2313 68-69 0.825655211786902 2313 70-71 0.8156913922905105 2313 72-73 0.7628426056967825 2313 74-75 0.8027521118008565 2313 76-77 0.9116559812868843 2313 78-79 0.9448589703545771 2313 80-81 0.9108599789879612 2313 82-83 0.9227756001864549 2313 84-85 0.8927202970073829 2313 86-87 0.8990815021641758 2313 88-89 0.9995180484994144 2313 90-91 0.9655032671501225 2313 92-93 0.9691961066062298 2313 94-95 1.0150327207668113 2313 96-97 0.9690740602206454 2313 98-99 1.0745450897405213 2313 100 1.1564221269590185 2314 1 0.3257421563549663 2314 2 0.2052940485474899 2314 3 0.2878001513582653 2314 4 0.2350558409774237 2314 5 0.20429481390485194 2314 6 0.2647779067681171 2314 7 0.2939337876589434 2314 8 0.2929018818546538 2314 9 0.27594191593836825 2314 10-11 0.40254575018862226 2314 12-13 0.38861676363070075 2314 14-15 0.42806247365621175 2314 16-17 0.4673822804336254 2314 18-19 0.3812688765463932 2314 20-21 0.41675133612176296 2314 22-23 0.48727637197254836 2314 24-25 0.5401846341474013 2314 26-27 0.5434398333264383 2314 28-29 0.4749611037767565 2314 30-31 0.6489243138010465 2314 32-33 0.6598117902512541 2314 34-35 0.702213623666168 2314 36-37 0.6220884165599614 2314 38-39 0.639837843237629 2314 40-41 0.678987118654554 2314 42-43 0.6587992383847237 2314 44-45 0.7534118480880494 2314 46-47 0.5179960337130112 2314 48-49 0.4777357455356608 2314 50-51 0.6625292010268637 2314 52-53 0.6166055622657751 2314 54-55 0.6304647161647168 2314 56-57 0.5897932388034164 2314 58-59 0.5626852927785677 2314 60-61 0.346120801738266 2314 62-63 0.5007378925097257 2314 64-65 0.5791890633437831 2314 66-67 0.6217871713904444 2314 68-69 0.5833451482662326 2314 70-71 0.6093888884520808 2314 72-73 0.6143880400932034 2314 74-75 0.5586800306821189 2314 76-77 0.5673194265788553 2314 78-79 0.6227341457004236 2314 80-81 0.585195086050593 2314 82-83 0.6225542478998776 2314 84-85 0.6991684148828234 2314 86-87 0.6441723027638915 2314 88-89 0.4879093116478579 2314 90-91 0.5465336490099482 2314 92-93 0.7573779641016785 2314 94-95 0.8327107675568683 2314 96-97 0.8017998593550963 2314 98-99 0.7640405074865697 2314 100 0.9585250331929274 2315 1 0.28622723487642787 2315 2 0.33311986667201765 2315 3 0.3179141663208185 2315 4 0.22636968592358642 2315 5 0.2305854443108757 2315 6 0.21377378216403287 2315 7 0.21444199539484288 2315 8 0.23717865140029204 2315 9 0.11529547265038786 2315 10-11 0.2531557494861829 2315 12-13 0.2594257344957427 2315 14-15 0.44659898332758274 2315 16-17 0.26814620330395655 2315 18-19 0.4172499255126283 2315 20-21 0.24769058939102706 2315 22-23 0.2295469560010872 2315 24-25 0.44546796107137965 2315 26-27 0.47099872094932493 2315 28-29 0.4300174842134368 2315 30-31 0.4444478503960454 2315 32-33 0.5305366260191491 2315 34-35 0.349317689653482 2315 36-37 0.5311877860626595 2315 38-39 0.5846446242089769 2315 40-41 0.6659705857111007 2315 42-43 0.7086427351981044 2315 44-45 0.7508965261740741 2315 46-47 0.7689781720699997 2315 48-49 0.7209840032049541 2315 50-51 0.625181552027378 2315 52-53 0.3167583944634842 2315 54-55 0.4200061282929468 2315 56-57 0.4607109503128939 2315 58-59 0.4900090386794602 2315 60-61 0.48744633431937956 2315 62-63 0.5002297085856426 2315 64-65 0.5620463388667787 2315 66-67 0.4000759111379679 2315 68-69 0.48388516065631393 2315 70-71 0.48567060926881567 2315 72-73 0.5312699060826631 2315 74-75 0.29831742428792296 2315 76-77 0.49318172203036426 2315 78-79 0.5075873279715744 2315 80-81 0.5555588949416865 2315 82-83 0.44000470046336204 2315 84-85 0.5800839158616888 2315 86-87 0.530741920389719 2315 88-89 0.49167237292718013 2315 90-91 0.57111240799345 2315 92-93 0.6944294337473593 2315 94-95 0.726154262306558 2315 96-97 0.7757310757382676 2315 98-99 0.8443768256315352 2315 100 0.9163400129707426 2316 1 0.13603406559925801 2316 2 0.20424507774052358 2316 3 0.18657360129682843 2316 4 0.1425832846445232 2316 5 0.10212466824307853 2316 6 -0.11074241554126729 2316 7 -0.10499668211878088 2316 8 -0.0023183845538312653 2316 9 0.04484546277267043 2316 10-11 0.11792499675262036 2316 12-13 0.09996179915885506 2316 14-15 -0.05966884994192867 2316 16-17 0.11665940909423966 2316 18-19 0.20991316943481308 2316 20-21 0.25519415213071994 2316 22-23 0.17165919447324285 2316 24-25 0.168267143121696 2316 26-27 0.04103235204301825 2316 28-29 0.08442436920862662 2316 30-31 -0.5296075831638447 2316 32-33 -0.32563505230466916 2316 34-35 -0.3963570774373153 2316 36-37 -0.11138004516334377 2316 38-39 -0.0532660783958363 2316 40-41 0.1134946581966787 2316 42-43 0.1975548495383066 2316 44-45 -0.07484155340885223 2316 46-47 -0.0877416111319036 2316 48-49 -0.007352197878979894 2316 50-51 0.15493988926969138 2316 52-53 -0.1828557749591866 2316 54-55 -0.10003253270098611 2316 56-57 -0.08407360213859505 2316 58-59 -0.5799363111122346 2316 60-61 -0.09422356565576706 2316 62-63 0.023185131839859707 2316 64-65 -0.30941571431090154 2316 66-67 -0.6339955684647762 2316 68-69 -0.5830236115662544 2316 70-71 -0.3787835414797911 2316 72-73 -0.6373153480126561 2316 74-75 -0.4311806408415464 2316 76-77 -0.16739027585970234 2316 78-79 -0.04542668405245465 2316 80-81 -0.2276050976435151 2316 82-83 -0.03687593192023897 2316 84-85 0.1012985784349425 2316 86-87 0.26688471917652024 2316 88-89 0.311949943165029 2316 90-91 -0.23330101691584204 2316 92-93 -0.3685361031204408 2316 94-95 -0.04363343136808595 2316 96-97 0.1931624275738848 2316 98-99 0.2774478619025551 2316 100 0.221124829384717 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 153.0 1 413.0 2 251.0 3 226.0 4 221.0 5 202.0 6 266.0 7 554.0 8 1501.0 9 2796.0 10 4463.0 11 7244.0 12 10999.0 13 13898.0 14 16073.0 15 18503.0 16 21398.0 17 25431.0 18 30261.0 19 36508.0 20 43871.0 21 53742.0 22 60108.0 23 61458.0 24 61455.0 25 63662.0 26 69513.0 27 79426.0 28 93015.0 29 110558.0 30 133841.0 31 165463.0 32 208523.0 33 270954.0 34 372853.0 35 519067.0 36 763684.0 37 1083718.0 38 995372.0 39 238038.0 40 183.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.65814476636661 19.496312134521073 13.729273713609953 23.116269385502363 2 15.593957605684478 19.91544851214835 40.93476662962192 23.55582725254525 3 19.034233334438746 25.79421694122579 29.29646621985526 25.8750835044802 4 12.748494786484105 16.653865708938675 38.42433126596831 32.173308238608904 5 13.526969453205137 37.59828290437864 34.57602152505558 14.29872611736064 6 30.86825230389017 37.092387809582334 17.905126076909337 14.134233809618163 7 27.600448794419343 32.345181446874186 22.050351895624253 18.004017863082225 8 25.890224518585303 35.3731579168623 19.945378502288253 18.791239062264147 9 26.315802542841706 16.280653697226846 20.704420680795376 36.69912307913607 10-11 25.163196858078017 26.26843730283835 27.705534565381 20.862831273702636 12-13 25.697702076455315 23.90371364580231 28.02796963157272 22.37061464616965 14-15 23.130832419017903 25.304597401464115 25.795729339929885 25.7688408395881 16-17 22.228327930579635 28.255321330295104 26.65924817188677 22.857102567238496 18-19 21.93072341621114 27.432749214318125 28.890610804469418 21.74591656500132 20-21 22.969830219592687 26.552606949168055 28.782522559215945 21.69504027202331 22-23 22.981366349444677 26.34718822415586 28.490525471393696 22.180919955005766 24-25 22.55784774940941 26.548978510797212 28.7748224196141 22.118351320179283 26-27 22.46083765326368 26.730199411219786 28.50171163536822 22.30725130014831 28-29 22.167268556109303 26.73367487609924 28.81507639475172 22.28398017303974 30-31 22.65710538098614 26.617309797860887 28.649619627653045 22.07596519349993 32-33 22.580352472185222 26.85449147396637 28.296588433529685 22.268567620318727 34-35 22.710916215351084 26.808017042863753 28.535763165996297 21.945303575788866 36-37 22.666530201517283 26.600229779403612 28.374226463442387 22.359013555636718 38-39 22.595181480115244 26.772513399880317 28.507821294010995 22.124483825993448 40-41 22.592307098676454 26.41030167179521 28.769096758684743 22.228294470843597 42-43 22.485989749456085 26.472070909160745 28.459092871481978 22.58284646990119 44-45 22.47622715869428 26.892289072002058 28.293657450204485 22.337826319099182 46-47 22.536971693394083 26.74352723141493 27.964962993932076 22.754538081258907 48-49 22.765113061657964 26.69794740762057 27.972093231203438 22.56484629951803 50-51 22.697127784711896 26.885360023268596 27.760008082626587 22.657504109392924 52-53 22.503315806641655 27.13726592303532 27.62899266277703 22.730425607545996 54-55 22.47273909708019 27.10458907055482 27.643168025724062 22.77950380664093 56-57 22.64305604674863 26.996938237227585 27.484450135631384 22.875555580392408 58-59 22.46520566147274 26.920761468349692 27.876158277484546 22.73787459269302 60-61 22.556468871871434 27.159403933378368 27.590513045033184 22.693614149717018 62-63 22.574352278291478 27.02389527168475 27.55322281910628 22.84852963091749 64-65 22.332978374727183 26.848326823294922 27.695038370567026 23.123656431410865 66-67 22.519014362977817 26.797456697563547 27.55048277225004 23.133046167208597 68-69 22.309563792546896 26.899301416265796 27.621516339982914 23.16961845120439 70-71 22.40441186580427 26.662089453470987 27.633762624481793 23.29973605624295 72-73 22.55631094048099 26.916743539223454 27.502409077558593 23.02453644273696 74-75 22.588249590428944 27.068908168270507 27.43893688037687 22.903905360923684 76-77 22.539986375538415 27.059200769214392 27.28037651767277 23.12043633757442 78-79 22.46328329546295 26.898012199780318 27.378892403586658 23.25981210117007 80-81 22.40402663523508 27.183291866030405 27.334621574157193 23.078059924577325 82-83 22.766916359539998 26.96110911534214 27.56918887514026 22.702785649977603 84-85 22.784045678868008 27.040430815423644 27.207615912309745 22.967907593398607 86-87 22.423529668486562 27.28429920487762 27.43360696665475 22.858564159981068 88-89 22.661778888014684 26.825773484052164 27.39276550682907 23.11968212110408 90-91 22.779045685027 26.87201884694223 27.367704783285447 22.981230684745324 92-93 22.760644623471034 26.98125792497847 27.343185237155815 22.91491221439469 94-95 22.39882117257945 26.843051298387937 27.536157175569816 23.221970353462794 96-97 22.65738632300614 26.77285574892217 27.33603057257571 23.23372735549598 98-99 22.485046705670207 26.925077764406304 27.2400572160764 23.34981831384709 100 22.95484578154236 26.730524667833823 26.824958806014454 23.489670744609363 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1502.0 1 1062.5 2 1047.0 3 1395.5 4 1069.5 5 921.0 6 1393.0 7 2490.5 8 4162.0 9 5124.5 10 4464.0 11 3725.0 12 3851.0 13 4239.5 14 4654.5 15 5191.5 16 5722.0 17 6216.0 18 6875.5 19 7415.0 20 7894.5 21 8828.5 22 10097.5 23 11660.0 24 13739.0 25 16519.5 26 20083.0 27 24731.0 28 30569.0 29 38832.0 30 50144.5 31 61682.0 32 76126.5 33 93575.5 34 110661.5 35 126516.0 36 145267.5 37 166983.0 38 177045.0 39 179301.0 40 178588.0 41 174861.5 42 176128.5 43 183031.5 44 191810.0 45 205534.5 46 218442.0 47 224638.0 48 229590.0 49 230712.0 50 225489.5 51 219551.0 52 213668.5 53 206034.5 54 196655.0 55 181050.5 56 163508.0 57 142783.5 58 123267.0 59 108796.0 60 90762.0 61 72702.5 62 58203.5 63 45766.5 64 34416.0 65 24659.5 66 18136.5 67 13909.0 68 10799.0 69 8284.0 70 6429.0 71 4905.0 72 3776.5 73 2966.0 74 2297.5 75 1739.0 76 1233.5 77 811.5 78 540.0 79 352.5 80 237.5 81 173.5 82 130.5 83 95.0 84 71.0 85 56.0 86 48.5 87 42.0 88 34.0 89 25.0 90 23.0 91 20.0 92 13.0 93 11.5 94 9.5 95 4.5 96 4.5 97 4.0 98 2.0 99 3.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02434455434660227 2 0.10883239226470846 3 0.016773451137571557 4 0.017092607713127886 5 0.018457888619674408 6 0.04524931004554187 7 0.04503653899517099 8 0.0382633272250311 9 0.03266035623193108 10-11 0.03620654040477919 12-13 0.03610015487959375 14-15 0.031330537167113044 16-17 0.03818353808114201 18-19 0.03700443184367002 20-21 0.047891217254313716 22-23 0.04783802449172099 24-25 0.06658847330565537 26-27 0.05844998062896896 28-29 0.05131328498111214 30-31 0.04888414882271118 32-33 0.04404360742677351 34-35 0.04520498274338127 36-37 0.0404974232539254 38-39 0.04600287418227209 40-41 0.055143163887788095 42-43 0.056535041175630976 44-45 0.05919467930526706 46-47 0.060719538499591746 48-49 0.05998370528372576 50-51 0.05467329448488572 52-53 0.06391110425515505 54-55 0.04979729124721957 56-57 0.05460237080142876 58-59 0.05305978068623984 60-61 0.049238767239995984 62-63 0.05647298295260614 64-65 0.05421229054241546 66-67 0.05216436918259568 68-69 0.057563434585756926 70-71 0.05758116550662117 72-73 0.059779799693787 74-75 0.05088774288037036 76-77 0.0522175619451884 78-79 0.06266107433422609 80-81 0.061996164801817064 82-83 0.052128907340867205 84-85 0.05847657701026532 86-87 0.05648184841303825 88-89 0.0643721081976253 90-91 0.06981550090294714 92-93 0.074523060392403 94-95 0.0786277685724747 96-97 0.07493087157228054 98-99 0.08396477575261109 100 0.13064142492772432 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5639865.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.161688038073315 #Duplication Level Percentage of deduplicated Percentage of total 1 74.80357325965778 45.003092385806944 2 14.762149644075489 17.762316833164494 3 5.024873215164982 9.069145644048783 4 2.0920986248863174 5.034567392611711 5 1.0703835564882567 3.2198040803264965 6 0.6158301413209119 2.222962850795478 7 0.380941855928922 1.6042673567928334 8 0.24656252135040807 1.1866893993091232 9 0.17739093370004952 0.9604924212640432 >10 0.7612172990336961 7.821387111347942 >50 0.04054209968269474 1.6623571524406184 >100 0.022418232316745897 2.464080563905836 >500 0.0015326534691935418 0.6070369555326649 >1k 3.738176342806775E-4 0.36489213582416474 >5k 3.738176342801144E-5 0.19685059259573479 >10k+ 7.476352685602288E-5 0.8200571242329343 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20439 0.3624022915442125 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16025 0.2841380068494547 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8753 0.1551987503246975 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 1.7730920864240545E-5 0.0 0.0 0.0 4 0.0 1.7730920864240545E-5 0.0 0.0 0.0 5 0.0 1.7730920864240545E-5 0.0 0.0 0.0 6 0.0 1.7730920864240545E-5 0.0 0.0 0.0 7 0.0 1.7730920864240545E-5 0.0 0.0 0.0 8 0.0 1.7730920864240545E-5 0.0 0.0 0.0 9 0.0 1.7730920864240545E-5 0.0 0.0 0.0 10-11 0.0 1.7730920864240545E-5 0.0 8.865460432120272E-6 0.0 12-13 0.0 1.7730920864240545E-5 0.0 1.7730920864240545E-5 1.7730920864240545E-5 14-15 0.0 1.7730920864240545E-5 0.0 1.7730920864240545E-5 3.546184172848109E-5 16-17 0.0 1.7730920864240545E-5 0.0 3.546184172848109E-5 8.865460432120272E-5 18-19 0.0 1.7730920864240545E-5 0.0 5.319276259272164E-5 1.0638552518544326E-4 20-21 0.0 1.7730920864240545E-5 0.0 7.092368345696218E-5 1.241164460496838E-4 22-23 0.0 1.7730920864240545E-5 0.0 2.1277105037088655E-4 1.241164460496838E-4 24-25 0.0 1.7730920864240545E-5 0.0 7.181022950017421E-4 1.5957828777816491E-4 26-27 0.0 1.7730920864240545E-5 0.0 0.001879477611609498 1.5957828777816491E-4 28-29 0.0 1.7730920864240545E-5 0.0 0.004290882849146212 1.5957828777816491E-4 30-31 0.0 1.7730920864240545E-5 0.0 0.012775128482685312 1.5957828777816491E-4 32-33 0.0 1.7730920864240545E-5 0.0 0.033325265764340105 1.5957828777816491E-4 34-35 0.0 1.7730920864240545E-5 0.0 0.06451395556453922 1.5957828777816491E-4 36-37 0.0 1.7730920864240545E-5 0.0 0.1144974214808333 1.5957828777816491E-4 38-39 0.0 1.7730920864240545E-5 0.0 0.1881339358300243 1.5957828777816491E-4 40-41 0.0 1.7730920864240545E-5 0.0 0.26912878233787507 1.95040129506646E-4 42-43 0.0 1.7730920864240545E-5 0.0 0.34874061701831516 1.95040129506646E-4 44-45 0.0 1.7730920864240545E-5 0.0 0.44861002878615 2.0390558993876626E-4 46-47 0.0 1.7730920864240545E-5 0.0 0.567141234763598 2.2163651080300682E-4 48-49 0.0 1.7730920864240545E-5 0.0 0.6957702001732311 2.3050197123512708E-4 50-51 0.0 1.7730920864240545E-5 0.0 0.8287698375758994 2.3050197123512708E-4 52-53 0.0 1.7730920864240545E-5 0.0 0.9599786519712794 2.6596381296360814E-4 54-55 0.0 1.7730920864240545E-5 0.0 1.1043970024105185 3.0142565469208925E-4 56-57 0.0 1.7730920864240545E-5 0.0 1.2798178679808827 3.0142565469208925E-4 58-59 0.0 1.7730920864240545E-5 0.0 1.4749909793940104 3.102911151242095E-4 60-61 0.0 1.7730920864240545E-5 0.0 1.6719815101957227 3.1915657555632983E-4 62-63 0.0 1.7730920864240545E-5 0.0 1.8710376932781192 3.3688749642057036E-4 64-65 0.0 1.7730920864240545E-5 0.0 2.0797838246128233 3.457529568526906E-4 66-67 0.0 1.7730920864240545E-5 0.0 2.3163675017043848 3.634838777169312E-4 68-69 0.0 1.7730920864240545E-5 0.0 2.5734215269337124 3.7234933814905147E-4 70-71 0.0 1.7730920864240545E-5 0.0 2.8493057901208627 3.8121479858117173E-4 72-73 0.0 1.7730920864240545E-5 0.0 3.127716709531168 4.4327302160601363E-4 74-75 0.0 1.7730920864240545E-5 0.0 3.40532087204215 4.6100394247025416E-4 76-77 0.0 1.7730920864240545E-5 0.0 3.710478885576162 4.6100394247025416E-4 78-79 0.0 1.7730920864240545E-5 0.0 4.042242145866966 4.6100394247025416E-4 80-81 0.0 1.7730920864240545E-5 0.0 4.396665523022271 5.230621654950961E-4 82-83 0.0 1.7730920864240545E-5 0.0 4.754112022184929 6.20582230248419E-4 84-85 0.0 1.7730920864240545E-5 0.0 5.105086735232138 6.383131511126597E-4 86-87 0.0 3.546184172848109E-5 0.0 5.4704500905606785 6.560440719769002E-4 88 0.0 3.546184172848109E-5 0.0 5.763134401266697 6.560440719769002E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9805 0.0 51.567905 1 GTATCAA 18540 0.0 42.011597 1 ATCAACG 22170 0.0 34.208073 3 CAACGCA 22195 0.0 34.16893 5 TATCAAC 23000 0.0 33.352276 2 TCAACGC 23360 0.0 32.54591 4 AACGCAG 23960 0.0 31.96482 6 ACGCAGA 26445 0.0 28.625322 7 CGCAGAG 27230 0.0 27.695332 8 GTACTAG 2310 0.0 27.0806 1 GTGGTAT 4685 0.0 25.801334 1 GCAGAGT 29540 0.0 25.102118 9 GTACATG 24410 0.0 24.952875 1 TACATGG 25060 0.0 24.06059 2 TGGTATC 4840 0.0 23.807842 2 ACATGGG 24680 0.0 23.265736 3 GAGTACT 16320 0.0 22.170267 12-13 CATGGGG 16735 0.0 21.591925 4 CAGAGTA 29520 0.0 20.749266 10-11 GTACTTT 17320 0.0 20.594263 14-15 >>END_MODULE