FastQCFastQC Report
Mon 6 Jun 2016
ERR1211293_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211293_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5639865
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175360.31092942827532216No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149320.2647581103448398No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85790.15211357009431964No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100600.052.6646961
GTATCAA178350.039.6518751
CAACGCA219900.031.3162965
ATCAACG220000.031.3081673
TATCAAC231700.030.2704852
TCAACGC231400.029.9279734
AACGCAG235450.029.6064136
ACGCAGA262150.026.2152187
CGCAGAG268500.025.5777578
GTACATG236350.025.3745781
GTGGTAT45650.024.6714151
TACATGG243950.024.32732
ACATGGG240150.023.6014463
GCAGAGT294400.022.9767869
TGGTATC46650.022.627422
GTACTAG24150.021.9576031
GAGTACT165050.021.3983212-13
CATGGGG161900.020.5182694
CAGAGTA290150.020.24711410-11
AGAGTAC277500.019.78410310-11
Produced by FastQC (version 0.11.5)