##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211293_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5639865 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.0476366012307 33.0 31.0 34.0 30.0 34.0 2 32.187147919320765 33.0 31.0 34.0 30.0 34.0 3 32.24581138023694 34.0 31.0 34.0 30.0 34.0 4 35.90246965131258 37.0 35.0 37.0 35.0 37.0 5 35.801351450788275 37.0 35.0 37.0 35.0 37.0 6 35.819267305157126 37.0 35.0 37.0 35.0 37.0 7 35.73620982062514 37.0 35.0 37.0 33.0 37.0 8 35.7184994676291 37.0 35.0 37.0 33.0 37.0 9 37.32394942077514 39.0 37.0 39.0 34.0 39.0 10-11 37.27234499407344 39.0 37.0 39.0 34.0 39.0 12-13 37.19875901284871 39.0 37.0 39.0 33.0 39.0 14-15 38.4094394103405 40.0 38.0 41.0 33.5 41.0 16-17 38.390003484125955 40.0 38.0 41.0 33.0 41.0 18-19 38.405816185316496 40.0 38.0 41.0 33.5 41.0 20-21 38.37728385342557 40.0 38.0 41.0 34.0 41.0 22-23 38.26670939818595 40.0 38.0 41.0 33.5 41.0 24-25 38.17188638380529 40.0 38.0 41.0 33.0 41.0 26-27 38.06879180618685 40.0 37.0 41.0 33.0 41.0 28-29 37.96851015050892 40.0 37.0 41.0 33.0 41.0 30-31 37.97432589255239 40.0 37.0 41.0 33.0 41.0 32-33 37.910600697002494 40.0 37.0 41.0 33.0 41.0 34-35 37.77783750142956 40.0 37.0 41.0 33.0 41.0 36-37 37.629240238906426 40.0 37.0 41.0 32.5 41.0 38-39 37.5133950014761 40.0 36.0 41.0 32.0 41.0 40-41 37.404576173365854 40.0 36.0 41.0 32.0 41.0 42-43 37.29675187260688 39.5 36.0 41.0 31.5 41.0 44-45 37.12765846345613 39.0 36.0 41.0 31.0 41.0 46-47 36.88048242289487 39.0 35.5 41.0 30.5 41.0 48-49 35.332583404035375 39.0 35.0 40.0 24.0 41.0 50-51 36.051509034347454 38.5 35.0 40.0 28.5 41.0 52-53 36.13862734303037 38.5 35.0 40.0 29.0 41.0 54-55 35.96854339598554 38.0 35.0 40.0 28.5 41.0 56-57 35.7486951372063 38.0 34.5 40.0 28.0 41.0 58-59 35.5067477678987 38.0 34.0 40.0 28.0 41.0 60-61 35.25212110573568 37.5 34.0 40.0 28.0 41.0 62-63 34.93104976803522 37.0 34.0 40.0 27.0 41.0 64-65 34.59861822933705 36.0 33.5 39.0 26.0 41.0 66-67 34.26194758207865 36.0 33.0 39.0 26.0 40.0 68-69 33.9060872024419 35.5 33.0 39.0 26.0 40.0 70-71 33.54992300347615 35.0 32.0 38.0 26.0 40.0 72-73 33.173478265880476 35.0 32.0 37.0 25.0 39.5 74-75 32.80958976854943 35.0 32.0 37.0 25.0 39.0 76-77 31.89953004194249 34.0 30.5 36.0 24.5 38.0 78-79 32.342777885640885 34.5 31.0 36.0 25.0 38.0 80-81 32.216953685948155 34.5 31.0 35.5 25.0 37.0 82-83 31.946034257912203 34.0 31.0 35.0 24.5 37.0 84-85 31.664900578293985 34.0 31.0 35.0 24.0 36.5 86-87 31.403684839974005 34.0 31.0 35.0 23.5 36.0 88-89 31.16735506966922 34.0 31.0 35.0 23.0 36.0 90-91 30.941756229980683 34.0 30.0 35.0 21.5 35.0 92-93 30.742541266501945 34.0 30.0 35.0 20.0 35.0 94-95 30.54160587177175 34.0 30.0 35.0 20.0 35.0 96-97 30.350049336287306 34.0 29.0 35.0 20.0 35.0 98-99 30.12343433752404 34.0 29.0 35.0 19.0 35.0 100 27.649117665050493 31.0 25.0 34.0 12.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.2800804868226727 1101 2 -0.21341605804363795 1101 3 -0.23463910455452108 1101 4 -0.17367353361264293 1101 5 -0.13449085544466044 1101 6 -0.15946398027567454 1101 7 -0.234322594226839 1101 8 -0.19055207769758908 1101 9 -0.2547063360805524 1101 10-11 -0.2741032703046926 1101 12-13 -0.3184788168767696 1101 14-15 -0.5259229804270547 1101 16-17 -0.43100060611006796 1101 18-19 -0.5031006166733931 1101 20-21 -0.4791808272890705 1101 22-23 -0.6706840969831234 1101 24-25 -0.5973909492932421 1101 26-27 -0.5888525768809956 1101 28-29 -0.674252144052069 1101 30-31 -0.5629277516765114 1101 32-33 -0.5204341022871972 1101 34-35 -0.5778291647722043 1101 36-37 -0.590489771800712 1101 38-39 -0.5576553691128439 1101 40-41 -0.5775524629536477 1101 42-43 -0.6331234390965292 1101 44-45 -0.6797899729300028 1101 46-47 -0.6837232861141658 1101 48-49 0.4725761637885668 1101 50-51 -0.43649922535826136 1101 52-53 -0.6505562554017672 1101 54-55 -0.736165175145679 1101 56-57 -0.7790872475911073 1101 58-59 -0.9122904377334962 1101 60-61 -0.8313499330426524 1101 62-63 -0.8535695860809724 1101 64-65 -1.014268409963968 1101 66-67 -0.8685943738531705 1101 68-69 -0.8493317762153438 1101 70-71 -0.8445342133096432 1101 72-73 -0.8346915712223009 1101 74-75 -0.9638289775520974 1101 76-77 -0.8897598153648012 1101 78-79 -0.7829810854158552 1101 80-81 -0.6766934927248016 1101 82-83 -0.6806426580914255 1101 84-85 -0.7667884486974401 1101 86-87 -0.6878670103561255 1101 88-89 -0.7674078903766492 1101 90-91 -0.754581476214188 1101 92-93 -0.8200654498326791 1101 94-95 -0.809316096017259 1101 96-97 -0.8593289561408106 1101 98-99 -1.0706097531454795 1101 100 -0.9430899581657712 1102 1 -0.25074005613089767 1102 2 -0.2658592682655012 1102 3 -0.2574455863556082 1102 4 -0.20537169973576397 1102 5 -0.1983290678089702 1102 6 -0.21576742991621956 1102 7 -0.20041718484053206 1102 8 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1105 68-69 -0.742198021169898 1105 70-71 -0.7256350701303944 1105 72-73 -0.7121162949165196 1105 74-75 -0.9186612622381816 1105 76-77 -0.8076601528197109 1105 78-79 -0.6173348466619757 1105 80-81 -0.5300123469914695 1105 82-83 -0.5576888309965682 1105 84-85 -0.5973131182495166 1105 86-87 -0.6510897910432227 1105 88-89 -0.7493099236639864 1105 90-91 -0.7484787507423896 1105 92-93 -0.667689016775924 1105 94-95 -0.7368673932871808 1105 96-97 -0.709514362434664 1105 98-99 -0.7690070535639748 1105 100 -0.949942256447688 1106 1 -0.30026608314186376 1106 2 -0.3150301850857993 1106 3 -0.3689437048666697 1106 4 -0.2589923530566409 1106 5 -0.2697614403346833 1106 6 -0.35684669351266507 1106 7 -0.38742191054642916 1106 8 -0.4069218316701395 1106 9 -0.5125968331656878 1106 10-11 -0.5188765298351967 1106 12-13 -0.508401795701495 1106 14-15 -0.6088014673798909 1106 16-17 -0.617620119464199 1106 18-19 -0.61137461569691 1106 20-21 -0.6552056096829233 1106 22-23 -0.6233352752511081 1106 24-25 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0.3913100633277935 2311 8 0.165693794910716 2311 9 0.4462469177267181 2311 10-11 0.3262465615314625 2311 12-13 0.3929467571575387 2311 14-15 0.4399778651952744 2311 16-17 0.45971828729847886 2311 18-19 0.6119515960219744 2311 20-21 0.6998640697258693 2311 22-23 0.6546579160084249 2311 24-25 0.5845509141753666 2311 26-27 0.6696539288613295 2311 28-29 0.7412752618583127 2311 30-31 0.6275881308323648 2311 32-33 0.6448402967086153 2311 34-35 0.7650273406556352 2311 36-37 0.7720002946270554 2311 38-39 0.8356215880205156 2311 40-41 0.7592201825863469 2311 42-43 0.7079021381742834 2311 44-45 0.8142743494584082 2311 46-47 0.8629282329653094 2311 48-49 -5.813745377753598 2311 50-51 -0.9844364132006191 2311 52-53 0.4477824405569919 2311 54-55 0.851247661827621 2311 56-57 0.9054736888854436 2311 58-59 0.9058097792569768 2311 60-61 0.757608423219601 2311 62-63 0.7546447291950997 2311 64-65 0.7607223847510411 2311 66-67 0.86513713610384 2311 68-69 0.8391879274354537 2311 70-71 0.8658236392096512 2311 72-73 0.343475483121054 2311 74-75 0.694817173301054 2311 76-77 0.7631783353079875 2311 78-79 0.625324073450571 2311 80-81 0.654833757148289 2311 82-83 0.7236685189007872 2311 84-85 0.7566418475616956 2311 86-87 0.7413113555394553 2311 88-89 0.8529479071397397 2311 90-91 0.8318529384077955 2311 92-93 0.756806616033483 2311 94-95 0.8322741803432727 2311 96-97 0.8967630319199138 2311 98-99 0.8778309207454598 2311 100 1.0427282184014146 2312 1 0.09611830841957669 2312 2 0.2317612463244032 2312 3 0.22151549901505518 2312 4 -0.012420817440805365 2312 5 0.16274521255380137 2312 6 0.07985744685822738 2312 7 0.2612541233219332 2312 8 0.3231674061081051 2312 9 0.23610950371442385 2312 10-11 0.22367450013050671 2312 12-13 0.331222652033766 2312 14-15 0.34121605633146146 2312 16-17 0.43029668621881 2312 18-19 0.2612393588277442 2312 20-21 0.3079420100614243 2312 22-23 0.5940040621597618 2312 24-25 0.7298093676660642 2312 26-27 0.6599861301220855 2312 28-29 0.7671459492658954 2312 30-31 0.7515793543756928 2312 32-33 0.8043706667608461 2312 34-35 0.3235826738621057 2312 36-37 0.6616749953729695 2312 38-39 0.7840757537557437 2312 40-41 0.5479026403855443 2312 42-43 0.6154690927751219 2312 44-45 0.6759613275583547 2312 46-47 0.7847553032126697 2312 48-49 -4.352431540917813 2312 50-51 -0.6742377934062844 2312 52-53 0.4949526667383495 2312 54-55 0.7937303322144871 2312 56-57 0.8737982475556763 2312 58-59 0.8554871434633 2312 60-61 0.9080431493625554 2312 62-63 0.7203585585956844 2312 64-65 0.9023528945554631 2312 66-67 0.9056265646851998 2312 68-69 0.881864279575737 2312 70-71 0.9180784210871664 2312 72-73 0.9844116099653561 2312 74-75 0.6802399004203679 2312 76-77 0.8515208900679063 2312 78-79 0.58635637591793 2312 80-81 0.6738684251793785 2312 82-83 0.6575742475663233 2312 84-85 0.7218601608208317 2312 86-87 0.8091414008402928 2312 88-89 0.8441001925061649 2312 90-91 0.7110734959197096 2312 92-93 0.9305700758025104 2312 94-95 1.0344149763877546 2312 96-97 1.0328603189078933 2312 98-99 1.0733020719718027 2312 100 0.6003718850643303 2313 1 0.10589116086689643 2313 2 0.28624034852906277 2313 3 0.33337710040362367 2313 4 0.12670522507620063 2313 5 0.19686750898407013 2313 6 0.347466067794997 2313 7 0.21357880622275616 2313 8 0.31246673210009845 2313 9 0.499226511523446 2313 10-11 0.2947763529454903 2313 12-13 0.0785612647209959 2313 14-15 0.16765203228172254 2313 16-17 0.07057934200496874 2313 18-19 0.4462864090061345 2313 20-21 0.3615140703420252 2313 22-23 0.45932425071519134 2313 24-25 0.32113078957800667 2313 26-27 0.44795968174306466 2313 28-29 0.45596731964818105 2313 30-31 0.6414642251225189 2313 32-33 0.5238082791973753 2313 34-35 0.5126378690828375 2313 36-37 -0.23188420406216181 2313 38-39 0.18535745228851397 2313 40-41 0.43150016111364664 2313 42-43 0.5664793977492479 2313 44-45 0.5853011750401365 2313 46-47 0.5033043525023473 2313 48-49 -5.119768588541291 2313 50-51 -1.1698841239908901 2313 52-53 0.16276424264969336 2313 54-55 0.41385157552222296 2313 56-57 0.43022459584731365 2313 58-59 0.5134138318507198 2313 60-61 0.8102093725545458 2313 62-63 0.8723639493175668 2313 64-65 0.9423709155575111 2313 66-67 1.0158723036613253 2313 68-69 1.0717695105316878 2313 70-71 0.9609369249437378 2313 72-73 0.665589010061332 2313 74-75 0.903369912878567 2313 76-77 0.8953595001187331 2313 78-79 0.8179669008158683 2313 80-81 -0.1679452368445098 2313 82-83 0.3104633840106459 2313 84-85 0.7104923094831932 2313 86-87 0.9150766594496993 2313 88-89 0.8902180794352219 2313 90-91 0.9126065393767142 2313 92-93 0.9996579935582908 2313 94-95 0.918395626160045 2313 96-97 0.9103438330104829 2313 98-99 1.0400481222829683 2313 100 1.2361222792063415 2314 1 0.13667520146201895 2314 2 0.20345174146802947 2314 3 0.20302560542560855 2314 4 0.04032275604100022 2314 5 0.20748951084637923 2314 6 0.35586570175518517 2314 7 0.1810595048480721 2314 8 0.2553702252150103 2314 9 0.40696239668322676 2314 10-11 0.32028632472048457 2314 12-13 0.24488822986928227 2314 14-15 0.23733602947081778 2314 16-17 0.471198566899254 2314 18-19 0.5432984997041146 2314 20-21 0.6713339790999839 2314 22-23 0.37704912252109324 2314 24-25 0.48741086903210373 2314 26-27 0.498559871138859 2314 28-29 0.638728619117984 2314 30-31 0.5482756756218947 2314 32-33 0.5159445284179256 2314 34-35 0.37003674552050825 2314 36-37 0.4962153049776319 2314 38-39 0.488435156213221 2314 40-41 0.22916617305314446 2314 42-43 0.28345541003390196 2314 44-45 0.49517439672981567 2314 46-47 0.625392623118266 2314 48-49 -6.49248276217066 2314 50-51 -1.648095053650657 2314 52-53 -0.8203392173675113 2314 54-55 -0.3667321973460176 2314 56-57 -0.06752897495105259 2314 58-59 0.3338078765880681 2314 60-61 0.6005412973644653 2314 62-63 0.415268046882197 2314 64-65 0.5839627714587508 2314 66-67 0.652185469852725 2314 68-69 0.6608317222001361 2314 70-71 0.4317229716584592 2314 72-73 0.5723462117821398 2314 74-75 0.6660536192083271 2314 76-77 0.5324985803343658 2314 78-79 0.371676995669894 2314 80-81 0.4476774171933755 2314 82-83 0.43997055141900887 2314 84-85 0.4307818497655802 2314 86-87 0.46475730345983735 2314 88-89 0.4417487363196706 2314 90-91 0.6570034363699158 2314 92-93 0.6749494614284863 2314 94-95 0.7312975633420358 2314 96-97 0.7957559548784694 2314 98-99 0.8193078002055572 2314 100 -0.5286486223322768 2315 1 0.07461604258281085 2315 2 0.2067274541657227 2315 3 0.29995161852924923 2315 4 0.12594767939670248 2315 5 0.23698719653591382 2315 6 0.2902046082449772 2315 7 0.3161530872847962 2315 8 0.26504417393308444 2315 9 0.38227112685046194 2315 10-11 0.23717791386975762 2315 12-13 0.4019363102992628 2315 14-15 0.22374702068941588 2315 16-17 0.38427847897014544 2315 18-19 0.4550421718121811 2315 20-21 0.3383135727671487 2315 22-23 0.4297379333865834 2315 24-25 0.4367069717912173 2315 26-27 0.5399079058898337 2315 28-29 0.637946599264481 2315 30-31 0.595655045934194 2315 32-33 0.6889193430528238 2315 34-35 0.6454805680795914 2315 36-37 0.618121901526429 2315 38-39 0.6524164448851053 2315 40-41 0.6472715697243316 2315 42-43 0.7113504411817502 2315 44-45 0.6896187419583484 2315 46-47 0.4981599177423206 2315 48-49 -4.747144620437155 2315 50-51 -0.9303042111210047 2315 52-53 0.17948199555103628 2315 54-55 0.1287197450436608 2315 56-57 0.5692606043615527 2315 58-59 0.703093486271861 2315 60-61 0.5964962644505718 2315 62-63 0.32516332646670065 2315 64-65 0.5160812257382759 2315 66-67 -0.07891181452826856 2315 68-69 0.5800985403893506 2315 70-71 0.5127480466954637 2315 72-73 0.621285256301114 2315 74-75 0.7311219968336289 2315 76-77 0.6786761680938191 2315 78-79 0.6356734842201774 2315 80-81 0.4954548417624949 2315 82-83 0.4812219498673542 2315 84-85 0.5321404331527901 2315 86-87 0.5693574692694803 2315 88-89 0.6021001114721152 2315 90-91 0.6337007925038591 2315 92-93 0.7419172285584139 2315 94-95 0.36888225900911564 2315 96-97 0.6019162314448216 2315 98-99 0.416650123294648 2315 100 0.423170144318636 2316 1 0.24405291202300106 2316 2 0.28462053344745897 2316 3 0.23545440339145074 2316 4 0.018239712737532443 2316 5 0.21201537128381176 2316 6 0.17927989454395288 2316 7 0.28495172419922454 2316 8 0.3388817874134631 2316 9 0.49750323047814504 2316 10-11 0.43255113861476957 2316 12-13 0.43424292930701114 2316 14-15 0.542470436414078 2316 16-17 0.4945947104338799 2316 18-19 0.20318527807584275 2316 20-21 0.5331293565268567 2316 22-23 0.6041316146906723 2316 24-25 0.6752830616564864 2316 26-27 0.6865501186822414 2316 28-29 0.702172649777765 2316 30-31 0.621961667771771 2316 32-33 0.6294422861361824 2316 34-35 0.6483176544932263 2316 36-37 0.4805623641269463 2316 38-39 0.6050027402637141 2316 40-41 0.15900532844086257 2316 42-43 0.1588023530970517 2316 44-45 0.39939759172998635 2316 46-47 0.4178727474347639 2316 48-49 -5.050731017016851 2316 50-51 -1.0236537783790993 2316 52-53 0.2507858127292266 2316 54-55 0.5309197308620597 2316 56-57 0.4162692471845233 2316 58-59 0.32771785292715805 2316 60-61 0.559368607252388 2316 62-63 0.3957180515146277 2316 64-65 0.42492269787236125 2316 66-67 0.545830187077911 2316 68-69 0.22782835748260055 2316 70-71 -0.03889289650234673 2316 72-73 0.06492648238876342 2316 74-75 0.26113482128162246 2316 76-77 0.30595999157509723 2316 78-79 0.35744234275662734 2316 80-81 0.26759995469772946 2316 82-83 0.14403403360599043 2316 84-85 0.2887346532511543 2316 86-87 0.3342264029656512 2316 88-89 0.12646200478084424 2316 90-91 0.2686430537257287 2316 92-93 0.4670056356948926 2316 94-95 0.6369248196892308 2316 96-97 0.6406150569068636 2316 98-99 0.6220416985353125 2316 100 -0.062484364463301745 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 4.0 10 8.0 11 6.0 12 27.0 13 139.0 14 434.0 15 1374.0 16 3091.0 17 6604.0 18 11020.0 19 16725.0 20 24662.0 21 35118.0 22 43539.0 23 46543.0 24 47971.0 25 53049.0 26 62982.0 27 77815.0 28 96259.0 29 117937.0 30 145263.0 31 178955.0 32 226145.0 33 292441.0 34 403256.0 35 564858.0 36 865765.0 37 1240096.0 38 959844.0 39 117921.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.58815044254223 20.2991228258427 14.63797344516061 23.474753286454465 2 15.315149823698235 20.39612664506055 40.40746754916253 23.881255982078674 3 18.6419133379351 25.725173014015773 29.976075651509852 25.656837996539277 4 12.767653910031719 17.07540569610544 38.28332977167872 31.873610622184113 5 13.388663596480107 37.73441083606938 34.68784791601025 14.189077651440266 6 30.976438438941077 37.38877391369721 17.591807178329123 14.042980469032587 7 27.521050607452914 32.43016095609448 21.98500991425143 18.06377852220117 8 25.975421931314322 35.635557591926606 19.6818178110674 18.707202665691668 9 26.097283828196733 16.162032608815156 20.596722946362107 37.143960616626 10-11 25.154943327817776 26.389596163270923 27.557048081812773 20.89841242709853 12-13 25.5966082849035 23.91516598681829 28.106032055225477 22.38219367305273 14-15 23.006906999528447 25.404821769907883 25.734590681663377 25.8536805489003 16-17 22.145963170412355 28.34537607934125 26.63262374175298 22.876037008493412 18-19 21.865438599228636 27.492932453690372 28.886911102820918 21.754717844260075 20-21 22.862573841573948 26.557992603187152 28.84658603761812 21.73284751762078 22-23 22.879366607043313 26.34865336796311 28.568234463056452 22.20374556193713 24-25 22.494376193180578 26.55497128210384 28.804476278945074 22.146176245770512 26-27 22.366215183748388 26.793214156595052 28.53842622661311 22.30214443304345 28-29 22.107355900061926 26.761268255879983 28.849173085711932 22.282202758346163 30-31 22.554814816916313 26.588005495312178 28.73655340091593 22.12062628685558 32-33 22.521656257140172 26.846116068725127 28.369415347333103 22.26281232680159 34-35 22.632642261370197 26.810224417497697 28.600178478449934 21.956954842682173 36-37 22.61659240820347 26.62840270497899 28.438094724631245 22.3169101621863 38-39 22.51882803485789 26.71140656318356 28.63559395076779 22.13417145119076 40-41 22.484759820948785 26.391834691567183 28.880692309608698 22.242713177875338 42-43 22.40224371577035 26.457763162409353 28.5289126917133 22.61108043010699 44-45 22.405576127591644 26.84420631813948 28.371346407129483 22.37887114713939 46-47 22.467314582250143 26.73556163520719 28.066013478478908 22.73111030406376 48-49 22.82864402163794 26.769442536396358 27.939788134129373 22.462125307836327 50-51 22.63266464628229 26.847123913854233 27.800312864601555 22.71989857526192 52-53 22.430640027225618 27.105937479328517 27.667075677532498 22.796346815913367 54-55 22.429774624642608 27.057624178636257 27.682299948814798 22.83030124790633 56-57 22.661565323229947 26.89624899114352 27.497843531867893 22.944342153758637 58-59 22.30285468716433 26.918269038525462 27.91786571365317 22.86101056065704 60-61 22.489797937635405 27.16982891885269 27.627436676640283 22.712936466871625 62-63 22.488652205846627 26.989964574383684 27.57781737206402 22.943565847705667 64-65 22.19337725606784 26.84201887806826 27.720775052624553 23.24382881323935 66-67 22.37990717446481 26.798817319995887 27.587400720955575 23.233874784583726 68-69 22.197574823684487 26.86849924124559 27.637593851074136 23.29633208399578 70-71 22.27264165801168 26.60001085420462 27.690929361120098 23.436418126663604 72-73 22.412240218445863 26.83205701739957 27.5161856498987 23.239517114255868 74-75 22.407636699103932 26.99259089814912 27.427180183523003 23.172592219223944 76-77 22.31790713067488 26.899137754081913 27.35938351345372 23.423571601789487 78-79 22.249278825522943 26.804913349122213 27.415154274949234 23.53065355040561 80-81 22.161581914993498 27.07891692878806 27.34054332292827 23.418957833290172 82-83 22.49386888660896 26.79164659463523 27.624222488637525 23.09026203011829 84-85 22.52122465411839 26.813331228750958 27.265695248195883 23.39974886893477 86-87 22.073348158075845 27.095972339680092 27.482321906895685 23.348357595348375 88-89 22.27137251340715 26.642548930159364 27.463641594342427 23.622436962091054 90-91 22.33947286071626 26.64422110811208 27.460644383025425 23.555661648146227 92-93 22.34053070528665 26.73674972426985 27.35788403808148 23.564835532362018 94-95 21.939767644727322 26.54858076778118 27.62088128038772 23.890770307103782 96-97 22.100501953885 26.491306260136355 27.523212164418027 23.88497962156062 98-99 21.875488510065914 26.606824045047333 27.43573612675747 24.08195131812928 100 21.985211551017027 26.394875248512385 27.389048942284823 24.230864258185765 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1351.0 1 930.0 2 810.5 3 1077.0 4 848.0 5 731.0 6 1138.5 7 2218.5 8 3686.0 9 4571.0 10 4045.5 11 3218.0 12 3272.0 13 3756.0 14 4371.0 15 4955.5 16 5493.0 17 6010.0 18 6554.0 19 7199.0 20 7867.0 21 8799.0 22 10105.5 23 11790.5 24 13867.5 25 16525.5 26 20309.0 27 24998.0 28 30454.0 29 38729.0 30 50526.0 31 62227.0 32 76609.0 33 93967.5 34 111028.0 35 127202.5 36 145610.5 37 166873.5 38 177778.5 39 179291.0 40 177253.5 41 174172.5 42 175388.0 43 181843.5 44 191231.5 45 205379.5 46 218835.5 47 225725.0 48 231252.0 49 232861.5 50 227885.0 51 222134.5 52 216073.5 53 208168.0 54 199335.0 55 183947.5 56 165677.0 57 144892.0 58 124824.5 59 109414.0 60 90654.5 61 71305.0 62 55868.0 63 43488.5 64 32391.0 65 22790.0 66 16479.0 67 12458.0 68 9606.5 69 7270.5 70 5485.5 71 4132.5 72 3159.0 73 2520.5 74 1932.5 75 1421.5 76 967.0 77 592.5 78 369.0 79 213.5 80 130.0 81 77.0 82 51.0 83 29.0 84 16.5 85 14.5 86 10.5 87 5.5 88 3.0 89 4.0 90 3.5 91 2.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029185095742539933 2 0.001826284849016776 3 7.092368345696218E-5 4 0.0195040129506646 5 6.028513093841786E-4 6 1.7730920864240545E-5 7 7.092368345696218E-5 8 0.0035993769354408303 9 0.00941511897891173 10-11 0.0010815861727186732 12-13 7.978914388908246E-5 14-15 1.1525098561756354E-4 16-17 4.344075611738933E-4 18-19 1.7730920864240545E-5 20-21 6.20582230248419E-5 22-23 3.014256546920893E-4 24-25 3.546184172848109E-5 26-27 1.0638552518544328E-4 28-29 0.0012854917626574395 30-31 0.002641907208771841 32-33 0.009920450223542585 34-35 0.019308972821157955 36-37 0.021738108979558905 38-39 0.021259374116224413 40-41 0.004423864755628016 42-43 0.005168563431926119 44-45 0.008289205504032454 46-47 0.011578291324349076 48-49 1.2005163244155668 50-51 0.01780184454769751 52-53 0.018759314274366494 54-55 0.009565831806257774 56-57 0.007721816036376757 58-59 0.008697016683909988 60-61 0.005656163755692734 62-63 0.007854797942858562 64-65 0.019495147490232477 66-67 0.029318077649021744 68-69 0.029734754289331394 70-71 0.026543188533768095 72-73 0.023147717188266033 74-75 0.009175751547244482 76-77 0.014406373202195445 78-79 0.008032107151500966 80-81 0.002544387144018518 82-83 0.014175871230960316 84-85 0.02438001618833075 86-87 0.023378219159501158 88-89 0.03242985426069596 90-91 0.021002275763692926 92-93 0.013236132425155568 94-95 0.006285611446373273 96-97 0.003608242395872951 98-99 0.016090810684298296 100 0.03219935228946083 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5639865.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.07578293696507 #Duplication Level Percentage of deduplicated Percentage of total 1 74.48066220968965 44.74484095910733 2 15.082055101323972 18.12132537020971 3 4.983940183310864 8.98242325870204 4 2.133431927755884 5.126703736106137 5 1.0629456053424664 3.1928644730177473 6 0.6165316517906456 2.222317301204601 7 0.38450414901238383 1.616957145610131 8 0.24908070097397517 1.197097450039971 9 0.17332657257072837 0.9371456595870482 >10 0.7689461233985028 7.841660877808174 >50 0.040274897199909336 1.6514018589499417 >100 0.022307515239324248 2.4913570819540465 >500 0.001504424703993691 0.588777132745046 >1k 3.7610591373868807E-4 0.3576765900310786 >5k 3.7610591373821475E-5 0.19384128021945876 >10k+ 7.522118274764295E-5 0.7336098247074702 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17536 0.31092942827532216 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14932 0.2647581103448398 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8579 0.15211357009431964 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.546184172848109E-5 2 0.0 0.0 0.0 0.0 3.546184172848109E-5 3 0.0 0.0 0.0 0.0 3.546184172848109E-5 4 0.0 0.0 0.0 0.0 3.546184172848109E-5 5 0.0 0.0 0.0 0.0 3.546184172848109E-5 6 0.0 0.0 0.0 0.0 3.546184172848109E-5 7 0.0 0.0 0.0 0.0 3.546184172848109E-5 8 0.0 0.0 0.0 0.0 3.546184172848109E-5 9 0.0 0.0 0.0 0.0 3.546184172848109E-5 10-11 0.0 0.0 0.0 0.0 3.546184172848109E-5 12-13 0.0 0.0 0.0 0.0 7.092368345696218E-5 14-15 0.0 0.0 0.0 0.0 7.978914388908246E-5 16-17 0.0 0.0 0.0 1.7730920864240545E-5 8.865460432120272E-5 18-19 0.0 0.0 0.0 3.546184172848109E-5 8.865460432120272E-5 20-21 0.0 0.0 0.0 5.319276259272163E-5 8.865460432120272E-5 22-23 0.0 0.0 0.0 1.6844374821028518E-4 1.0638552518544326E-4 24-25 0.0 0.0 0.0 6.294476906805393E-4 1.241164460496838E-4 26-27 0.0 0.0 0.0 0.0017996884677204152 1.4184736691392436E-4 28-29 0.0 0.0 0.0 0.004193362784392889 1.4184736691392436E-4 30-31 0.0 0.0 0.0 0.012730801180524711 1.4184736691392436E-4 32-33 0.0 0.0 0.0 0.03322774569958678 1.5957828777816491E-4 34-35 0.0 0.0 0.0 0.06433664635589681 2.3050197123512708E-4 36-37 0.0 0.0 0.0 0.11377045372539946 2.3050197123512708E-4 38-39 0.0 0.0 0.0 0.18459661711760833 2.3050197123512708E-4 40-41 0.0 0.0 0.0 0.262116203136068 2.3050197123512708E-4 42-43 0.0 0.0 0.0 0.3376321950968685 2.3050197123512708E-4 44-45 0.0 0.0 0.0 0.4332550513177177 2.3050197123512708E-4 46-47 0.0 0.0 0.0 0.5467506757697214 2.570983525314879E-4 48-49 0.0 0.0 0.0 0.6708227945172447 2.659638129636082E-4 50-51 0.0 0.0 0.0 0.8035121408047887 2.659638129636082E-4 52-53 0.0 0.0 0.0 0.9342510857972663 3.1915657555632983E-4 54-55 0.0 0.0 0.0 1.0787226289990983 3.3688749642057036E-4 56-57 0.0 0.0 0.0 1.2536824906269919 3.3688749642057036E-4 58-59 0.0 0.0 1.7730920864240545E-5 1.4480399796803647 3.3688749642057036E-4 60-61 0.0 0.0 1.7730920864240545E-5 1.644427659172693 3.3688749642057036E-4 62-63 0.0 0.0 1.7730920864240545E-5 1.8432356093629902 3.7234933814905147E-4 64-65 0.0 0.0 1.7730920864240545E-5 2.0517778351077554 3.7234933814905147E-4 66-67 0.0 0.0 1.7730920864240545E-5 2.2872887914870303 3.7234933814905147E-4 68-69 0.0 0.0 1.7730920864240545E-5 2.5439438709969124 3.7234933814905147E-4 70-71 0.0 0.0 1.7730920864240545E-5 2.819526708529371 3.9894571944541226E-4 72-73 0.0 0.0 1.7730920864240545E-5 3.097467758536774 4.787348633344947E-4 74-75 0.0 0.0 1.7730920864240545E-5 3.374362684213186 5.053312446308554E-4 76-77 0.0 0.0 1.7730920864240545E-5 3.678855788214789 5.230621654950961E-4 78-79 0.0 0.0 1.7730920864240545E-5 4.009519731412011 5.319276259272164E-4 80-81 0.0 0.0 1.7730920864240545E-5 4.363402315480956 5.496585467914569E-4 82-83 0.0 0.0 1.7730920864240545E-5 4.720636043593242 5.673894676556974E-4 84-85 1.7730920864240545E-5 0.0 1.7730920864240545E-5 5.07206289512249 5.673894676556974E-4 86-87 1.7730920864240545E-5 0.0 1.7730920864240545E-5 5.437913850774796 6.028513093841785E-4 88 1.7730920864240545E-5 0.0 1.7730920864240545E-5 5.730970510818965 6.20582230248419E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10060 0.0 52.664696 1 GTATCAA 17835 0.0 39.651875 1 CAACGCA 21990 0.0 31.316296 5 ATCAACG 22000 0.0 31.308167 3 TATCAAC 23170 0.0 30.270485 2 TCAACGC 23140 0.0 29.927973 4 AACGCAG 23545 0.0 29.606413 6 ACGCAGA 26215 0.0 26.215218 7 CGCAGAG 26850 0.0 25.577757 8 GTACATG 23635 0.0 25.374578 1 GTGGTAT 4565 0.0 24.671415 1 TACATGG 24395 0.0 24.3273 2 ACATGGG 24015 0.0 23.601446 3 GCAGAGT 29440 0.0 22.976786 9 TGGTATC 4665 0.0 22.62742 2 GTACTAG 2415 0.0 21.957603 1 GAGTACT 16505 0.0 21.39832 12-13 CATGGGG 16190 0.0 20.518269 4 CAGAGTA 29015 0.0 20.247114 10-11 AGAGTAC 27750 0.0 19.784103 10-11 >>END_MODULE