FastQCFastQC Report
Mon 6 Jun 2016
ERR1211292_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211292_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4328916
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177140.40920174935249376No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136290.31483632392035327No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78790.182008613703754No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78300.053.653791
GTATCAA146550.041.2182851
CAACGCA175450.033.5347145
ATCAACG175100.033.5212333
TACCTGG30900.032.7314762
TATCAAC182450.032.564552
TCAACGC182950.032.1339074
AACGCAG186200.031.8261476
ACGCAGA208800.028.088457
GTACATG184500.027.8698431
CGCAGAG213650.027.3631538
TACATGG194350.026.3832042
GTACCTG40650.025.3451141
ACCTGGG39200.025.2953113
ACATGGG197000.025.1430533
TGGTATC38200.024.87562
GTGGTAT38400.024.7474751
GCAGAGT230300.024.6097439
CATGGGG133100.022.7376964
GTACTAG17150.022.4936771
Produced by FastQC (version 0.11.5)