##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211292_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4328916 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.44503173542753 31.0 31.0 34.0 28.0 34.0 2 31.46010571699705 31.0 31.0 34.0 28.0 34.0 3 31.507172003337555 33.0 31.0 34.0 28.0 34.0 4 35.11802123210522 37.0 35.0 37.0 32.0 37.0 5 35.16599698400246 37.0 35.0 37.0 32.0 37.0 6 35.23373126205267 37.0 35.0 37.0 32.0 37.0 7 35.169724245053494 37.0 35.0 37.0 32.0 37.0 8 35.16707508299999 37.0 35.0 37.0 32.0 37.0 9 36.73371231966617 39.0 37.0 39.0 32.0 39.0 10-11 36.66514492311701 39.0 37.0 39.0 32.0 39.0 12-13 36.583826643898846 39.0 37.0 39.0 32.0 39.0 14-15 37.722420000757694 40.0 37.0 41.0 32.0 41.0 16-17 37.71940989383947 40.0 37.0 41.0 32.0 41.0 18-19 37.731603015627925 40.0 37.0 41.0 32.0 41.0 20-21 37.69723159331343 40.0 37.0 41.0 32.0 41.0 22-23 37.57796536130523 40.0 37.0 41.0 32.0 41.0 24-25 37.47311082035318 40.0 37.0 41.0 31.0 41.0 26-27 37.32626354958147 40.0 37.0 41.0 31.0 41.0 28-29 37.18893956362285 40.0 36.0 41.0 31.0 41.0 30-31 36.99605501700657 39.5 36.0 41.0 30.0 41.0 32-33 36.85927423863156 39.0 36.0 41.0 30.0 41.0 34-35 36.71365279437161 39.0 35.0 41.0 30.0 41.0 36-37 36.620122450978485 39.0 35.0 41.0 30.0 41.0 38-39 36.46982662634248 39.0 35.0 41.0 29.5 41.0 40-41 36.30205760518338 39.0 35.0 41.0 29.0 41.0 42-43 36.156266256956705 39.0 35.0 40.0 28.0 41.0 44-45 36.01801397855722 39.0 35.0 40.0 27.5 41.0 46-47 35.84576623801432 39.0 35.0 40.0 27.0 41.0 48-49 35.60345465238873 39.0 34.5 40.0 26.0 41.0 50-51 34.93484904765997 37.5 33.5 39.5 25.0 40.5 52-53 35.17019907524193 38.0 34.0 40.0 26.0 40.5 54-55 35.5309252939997 38.5 34.0 40.0 26.0 41.0 56-57 35.43944534844289 38.0 34.0 40.0 26.0 41.0 58-59 35.22827031062742 38.0 34.0 40.0 26.0 41.0 60-61 34.97056872898435 38.0 34.0 40.0 26.0 41.0 62-63 34.69914396583348 37.0 34.0 40.0 25.5 41.0 64-65 34.42878944751989 37.0 33.0 40.0 25.0 41.0 66-67 34.21159084629963 36.0 33.0 39.5 25.0 41.0 68-69 33.880391534508874 36.0 33.0 39.0 24.0 41.0 70-71 33.54082557850511 35.0 32.5 39.0 24.0 40.5 72-73 33.17677381589294 35.0 32.0 38.0 23.0 40.0 74-75 32.805889626871945 35.0 32.0 37.0 22.5 39.5 76-77 32.4497415750271 35.0 31.5 37.0 22.0 39.0 78-79 32.077745098310984 35.0 31.0 36.0 21.0 39.0 80-81 31.743496062293655 35.0 31.0 36.0 20.0 38.0 82-83 31.426906759105513 35.0 31.0 35.5 20.0 37.0 84-85 31.117304424479478 34.0 30.0 35.0 20.0 37.0 86-87 30.85797010152195 34.0 30.0 35.0 19.0 36.0 88-89 30.600985674011692 34.0 30.0 35.0 18.0 36.0 90-91 30.34234598222742 34.0 29.0 35.0 18.0 36.0 92-93 30.106271754868885 34.0 29.0 35.0 17.5 35.0 94-95 29.884546385284445 34.0 29.0 35.0 16.5 35.0 96-97 29.678579695240103 34.0 29.0 35.0 15.0 35.0 98-99 29.44418071406329 34.0 28.5 35.0 14.0 35.0 100 27.769155372846228 32.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.4586679289320372 1101 2 -0.48705193345843156 1101 3 -0.38791218938857597 1101 4 -0.24266689439594558 1101 5 -0.3311271547035304 1101 6 -0.33525907178862013 1101 7 -0.42021666929117885 1101 8 -0.4501145041669332 1101 9 -0.4854112489700242 1101 10-11 -0.5511284220885386 1101 12-13 -0.5141912681326559 1101 14-15 -0.5533300025907195 1101 16-17 -0.6049078387218216 1101 18-19 -0.5682423141403845 1101 20-21 -0.5729998626514643 1101 22-23 -0.620063616251258 1101 24-25 -0.5588020342352067 1101 26-27 -0.7087974196236431 1101 28-29 -0.7045100647994005 1101 30-31 -0.7180495812482022 1101 32-33 -0.7330332491997993 1101 34-35 -0.8380564900382907 1101 36-37 -0.8817121524139182 1101 38-39 -0.8874016156995808 1101 40-41 -0.9062462777904727 1101 42-43 -0.8521302006480624 1101 44-45 -1.0001262810131735 1101 46-47 -0.8714949359387063 1101 48-49 -0.9565857805378286 1101 50-51 -0.7529458151976129 1101 52-53 -0.6408455628307408 1101 54-55 -0.6763722085830111 1101 56-57 -0.6128975006505968 1101 58-59 -0.6778695319532986 1101 60-61 -0.665873118266525 1101 62-63 -0.7296584876450822 1101 64-65 -0.7207463523383026 1101 66-67 -0.7365400342545314 1101 68-69 -0.6859550348313164 1101 70-71 -0.6751796325370236 1101 72-73 -0.8365292049853323 1101 74-75 -0.9488706592890388 1101 76-77 -0.8615805203706302 1101 78-79 -0.8448157757899253 1101 80-81 -0.9730566824368481 1101 82-83 -0.99732839346958 1101 84-85 -0.9545094041396887 1101 86-87 -1.0085232553490577 1101 88-89 -1.0108202116154068 1101 90-91 -1.0546951182202768 1101 92-93 -1.2605757056800329 1101 94-95 -1.1906406789741233 1101 96-97 -1.1931587624388236 1101 98-99 -1.2834668894316899 1101 100 -1.3922101288793058 1102 1 -0.3337036657659951 1102 2 -0.41040076764349465 1102 3 -0.44144919289475837 1102 4 -0.30878227076436815 1102 5 -0.2287401327333214 1102 6 -0.2608789321370182 1102 7 -0.306852607244096 1102 8 -0.28487296784044247 1102 9 -0.3454995586574867 1102 10-11 -0.4246521061788968 1102 12-13 -0.5083594635416588 1102 14-15 -0.5524606544702877 1102 16-17 -0.6224345222245091 1102 18-19 -0.6237469529799711 1102 20-21 -0.6182132860019109 1102 22-23 -0.6290039049651668 1102 24-25 -0.6572659459890531 1102 26-27 -0.6651622490199642 1102 28-29 -0.7304047513278036 1102 30-31 -0.7084405918461556 1102 32-33 -0.8072651058077369 1102 34-35 -0.6846344772405359 1102 36-37 -0.7261765168343288 1102 38-39 -0.8823487624112047 1102 40-41 -0.8507941894688784 1102 42-43 -0.7968785115863071 1102 44-45 -0.7798308490739743 1102 46-47 -0.8580161313556545 1102 48-49 -0.8138421322637157 1102 50-51 -0.8178829307784738 1102 52-53 -0.7015635604959343 1102 54-55 -0.6374832121527234 1102 56-57 -0.6529242437146721 1102 58-59 -0.6514161471787787 1102 60-61 -0.8026249527573341 1102 62-63 -0.716810041105397 1102 64-65 -0.7243202839062377 1102 66-67 -0.6461683438824224 1102 68-69 -0.7212253045649959 1102 70-71 -0.7513191908297543 1102 72-73 -0.8603023892563613 1102 74-75 -0.8538154582892012 1102 76-77 -0.7850145397519057 1102 78-79 -0.7483608200837466 1102 80-81 -0.8055737765557609 1102 82-83 -0.8683147623491507 1102 84-85 -0.8204906308859314 1102 86-87 -0.9606921089114202 1102 88-89 -0.898493014097923 1102 90-91 -0.9821791259870771 1102 92-93 -0.9420827751206708 1102 94-95 -0.950670879080544 1102 96-97 -1.0827921273396974 1102 98-99 -1.005836919033701 1102 100 -1.0579985132170435 1103 1 -0.49236157684772053 1103 2 -0.5425288305955469 1103 3 -0.4955076669786287 1103 4 -0.4208489995415903 1103 5 -0.31840874285934717 1103 6 -0.42571997419187113 1103 7 -0.40388394803069616 1103 8 -0.4595850804007995 1103 9 -0.6144542980936833 1103 10-11 -0.6164916430545375 1103 12-13 -0.7573008291724861 1103 14-15 -0.7709007278008144 1103 16-17 -0.866861351981477 1103 18-19 -0.7186407389189426 1103 20-21 -0.8803247193532755 1103 22-23 -0.8343129801189448 1103 24-25 -0.8290397032770258 1103 26-27 -0.7713928394709697 1103 28-29 -0.8631108431712846 1103 30-31 -0.7595898875925826 1103 32-33 -0.8529729657985214 1103 34-35 -0.9319321045170952 1103 36-37 -0.9656958612674131 1103 38-39 -0.9764374277354833 1103 40-41 -0.941651227496564 1103 42-43 -0.8183724583114795 1103 44-45 -0.7754160532373859 1103 46-47 -0.8333373906972739 1103 48-49 -0.8952755211960124 1103 50-51 -0.8567481734103595 1103 52-53 -0.7268862599008727 1103 54-55 -0.749988596737289 1103 56-57 -0.6910195533658552 1103 58-59 -0.7139219700434793 1103 60-61 -0.759996449020683 1103 62-63 -0.8122330816175065 1103 64-65 -0.8506734425285813 1103 66-67 -0.8304298919379676 1103 68-69 -0.8068326857034265 1103 70-71 -0.8238956268859354 1103 72-73 -0.8824550710299164 1103 74-75 -0.8164044291573447 1103 76-77 -0.7611414832112615 1103 78-79 -0.7995406702169845 1103 80-81 -0.7561755977975686 1103 82-83 -0.777312841429918 1103 84-85 -0.7985336098885547 1103 86-87 -0.8618282562536912 1103 88-89 -0.9993436532355879 1103 90-91 -0.941495709505741 1103 92-93 -0.8794462195517561 1103 94-95 -1.123162727069964 1103 96-97 -1.0934341952205244 1103 98-99 -1.01478115226886 1103 100 -0.8636347958000385 1104 1 -0.3774203720356972 1104 2 -0.3849660078242714 1104 3 -0.4477924735793479 1104 4 -0.27517695774506024 1104 5 -0.2158555561752138 1104 6 -0.3221233799464258 1104 7 -0.33773860873503736 1104 8 -0.3460591758883993 1104 9 -0.4192505099069237 1104 10-11 -0.4597345251479723 1104 12-13 -0.5045632017578185 1104 14-15 -0.5512017049602704 1104 16-17 -0.5885263624456627 1104 18-19 -0.5247476091479513 1104 20-21 -0.6706412045904884 1104 22-23 -0.6137766340027397 1104 24-25 -0.6413131046652225 1104 26-27 -0.6988122125230447 1104 28-29 -0.7097485639355483 1104 30-31 -0.7238721873050054 1104 32-33 -0.6831299662652697 1104 34-35 -0.7192009889894351 1104 36-37 -0.7157506288720938 1104 38-39 -0.7597446005978483 1104 40-41 -0.6332917336922677 1104 42-43 -0.6924703925753022 1104 44-45 -0.6640878164907633 1104 46-47 -0.8134076287387515 1104 48-49 -0.6907138125973091 1104 50-51 -0.5506262467951828 1104 52-53 -0.5242035882957126 1104 54-55 -0.5897028265278479 1104 56-57 -0.58231338627008 1104 58-59 -0.6146384658201569 1104 60-61 -0.5022237778672078 1104 62-63 -0.5741985799086109 1104 64-65 -0.6297000848039076 1104 66-67 -0.6033502285987566 1104 68-69 -0.5782447488800244 1104 70-71 -0.6301513671331591 1104 72-73 -0.5848719974192491 1104 74-75 -0.5392875012623861 1104 76-77 -0.5463841545152981 1104 78-79 -0.604450369540956 1104 80-81 -0.5598679882999988 1104 82-83 -0.7614193201928856 1104 84-85 -0.6512456414467387 1104 86-87 -0.6529459294397704 1104 88-89 -0.5253066973357434 1104 90-91 -0.6430664669925292 1104 92-93 -0.5815439505543658 1104 94-95 -0.5075097316019352 1104 96-97 -0.6269713543815811 1104 98-99 -0.675068804526564 1104 100 -0.8439489472973989 1105 1 -0.42560553885724417 1105 2 -0.5148681505308446 1105 3 -0.41670041666614566 1105 4 -0.36712253452216714 1105 5 -0.3135912454862222 1105 6 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68-69 -0.6858366806015965 1105 70-71 -0.6626142846818581 1105 72-73 -0.7244719136942592 1105 74-75 -0.8106775785644942 1105 76-77 -0.7626544758866451 1105 78-79 -0.6951852864246035 1105 80-81 -0.7986605546886736 1105 82-83 -0.817220393179106 1105 84-85 -0.8077993733941575 1105 86-87 -0.8922010368126188 1105 88-89 -0.8441400079069616 1105 90-91 -0.9573925698911374 1105 92-93 -0.932112038467583 1105 94-95 -1.0283488173171271 1105 96-97 -1.017435055738698 1105 98-99 -1.1027559214674163 1105 100 -1.0009101705531513 1106 1 -0.4107256185064081 1106 2 -0.43549689318621176 1106 3 -0.4476144445882717 1106 4 -0.34729082644601306 1106 5 -0.27812895728568776 1106 6 -0.2984829975382084 1106 7 -0.2561102546833922 1106 8 -0.32857862082602196 1106 9 -0.37863200711618106 1106 10-11 -0.36880518713446264 1106 12-13 -0.333519180572857 1106 14-15 -0.4939944898867026 1106 16-17 -0.6321819088491409 1106 18-19 -0.5950050283970043 1106 20-21 -0.602863578808396 1106 22-23 -0.5407883252199426 1106 24-25 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1.0129767358439103 2212 90-91 1.0450316906818315 2212 92-93 1.1096009769151856 2212 94-95 1.1577432124074392 2212 96-97 1.1054422348405346 2212 98-99 1.130751576392509 2212 100 1.1661499481047635 2213 1 0.44423230444703066 2213 2 0.44669538385501184 2213 3 0.47381892039340556 2213 4 0.43316898968853934 2213 5 0.4176555212983004 2213 6 0.46156773514003646 2213 7 0.48800906339009487 2213 8 0.4876973031721974 2213 9 0.569876135659797 2213 10-11 0.5707583421516915 2213 12-13 0.5720714709636141 2213 14-15 0.7170038819394122 2213 16-17 0.679926919513882 2213 18-19 0.6605237086815094 2213 20-21 0.6398732213532767 2213 22-23 0.7354596395626984 2213 24-25 0.844178386550773 2213 26-27 0.8547684718492334 2213 28-29 0.8662880857594217 2213 30-31 0.794157375559081 2213 32-33 0.854184262439837 2213 34-35 0.8146179872043149 2213 36-37 0.8722975111214808 2213 38-39 0.86577143077686 2213 40-41 0.8645111193878634 2213 42-43 0.7858408087457391 2213 44-45 0.9477539568223818 2213 46-47 0.9107443597021501 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0.6178764495315576 2215 34-35 0.6282661735631834 2215 36-37 0.7116245552033078 2215 38-39 0.7365449951966383 2215 40-41 0.8986591045147208 2215 42-43 0.7142047623842913 2215 44-45 0.7410862689783002 2215 46-47 0.693600705568933 2215 48-49 0.6665071971026109 2215 50-51 0.5172237371949393 2215 52-53 0.576175012876412 2215 54-55 0.6401375255519426 2215 56-57 0.5854618881331461 2215 58-59 0.5943470599496337 2215 60-61 0.6566440428099085 2215 62-63 0.7029780472721185 2215 64-65 0.5974319134048258 2215 66-67 0.6305906013319955 2215 68-69 0.683651948041117 2215 70-71 0.6603320872868252 2215 72-73 0.686870584351503 2215 74-75 0.780689523223181 2215 76-77 0.6817726288209798 2215 78-79 0.6614497047387786 2215 80-81 0.6998316010217565 2215 82-83 0.7231573197498875 2215 84-85 0.6530290919066175 2215 86-87 0.6581349609540723 2215 88-89 0.8068589942184552 2215 90-91 0.8132451891932071 2215 92-93 0.83425564167133 2215 94-95 0.9628352144344525 2215 96-97 0.9428921394250018 2215 98-99 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0.5095576337529195 2311 8 0.6227929948927837 2311 9 0.7109972734396166 2311 10-11 0.7417353804427265 2311 12-13 0.7280760546974605 2311 14-15 0.8612774426182881 2311 16-17 0.8979061791344591 2311 18-19 0.8227744965481207 2311 20-21 0.8307498694172111 2311 22-23 0.8710426967242739 2311 24-25 0.92762057459624 2311 26-27 0.9730347827205961 2311 28-29 0.933128166769805 2311 30-31 0.9827569815062134 2311 32-33 1.010498911737571 2311 34-35 0.9776422951011554 2311 36-37 0.9636462813800506 2311 38-39 0.9832254260616153 2311 40-41 1.052743273431929 2311 42-43 1.044552055033563 2311 44-45 1.091813753387811 2311 46-47 1.1332897188210822 2311 48-49 1.1257819889326512 2311 50-51 1.079145778898166 2311 52-53 0.9236095387218484 2311 54-55 0.9463385534370374 2311 56-57 0.9738438881374449 2311 58-59 0.9402954969490693 2311 60-61 0.9695114569149368 2311 62-63 0.9131961997766638 2311 64-65 0.9664482098300127 2311 66-67 0.9693968885653845 2311 68-69 0.923685811187724 2311 70-71 0.8734135451923706 2311 72-73 0.8386902653863544 2311 74-75 0.9080319207224861 2311 76-77 1.0136953827951771 2311 78-79 0.9050828069505386 2311 80-81 1.0440288592884173 2311 82-83 1.0140042075440334 2311 84-85 1.1050649917976507 2311 86-87 1.0986312345579066 2311 88-89 1.0456305482829684 2311 90-91 1.1214010981382039 2311 92-93 1.2322043809477314 2311 94-95 1.2276570634341262 2311 96-97 1.1762317509409428 2311 98-99 1.2000729493521973 2311 100 1.3356153450830597 2312 1 0.4978405908060779 2312 2 0.4983274453417579 2312 3 0.5020606184549514 2312 4 0.41002317582947256 2312 5 0.4267109367965105 2312 6 0.4502266309845879 2312 7 0.43253691277022455 2312 8 0.43299208454110527 2312 9 0.59119821404785 2312 10-11 0.5634132392803579 2312 12-13 0.5352163642210357 2312 14-15 0.8100639618193028 2312 16-17 0.7384528710383051 2312 18-19 0.7073879978354043 2312 20-21 0.6721912963044119 2312 22-23 0.7002774312517914 2312 24-25 0.7757305917458055 2312 26-27 0.759432381431985 2312 28-29 0.8395138031205533 2312 30-31 0.8276448506556235 2312 32-33 0.8172473637815543 2312 34-35 0.8340908365372357 2312 36-37 0.7894867962625014 2312 38-39 0.8672579663876192 2312 40-41 0.8817031191234861 2312 42-43 0.9001073016783536 2312 44-45 0.8533115581593762 2312 46-47 0.9417590751654288 2312 48-49 0.980486520378058 2312 50-51 1.0174535231491078 2312 52-53 0.8847462059370415 2312 54-55 0.8538072768482721 2312 56-57 0.774203147182412 2312 58-59 0.774315238350674 2312 60-61 0.8105699179432122 2312 62-63 0.8464649624112184 2312 64-65 0.7960067371071204 2312 66-67 0.819757925934482 2312 68-69 0.7879882209672076 2312 70-71 0.8148940325934433 2312 72-73 0.8150537077509981 2312 74-75 0.8441065532293024 2312 76-77 0.8929509938461351 2312 78-79 0.8686438809881807 2312 80-81 0.8400497588549314 2312 82-83 0.9581534334846182 2312 84-85 0.9754006166644267 2312 86-87 0.9282809322553298 2312 88-89 0.9016016347363198 2312 90-91 0.935297160853434 2312 92-93 1.1012384478773356 2312 94-95 1.0397647832894634 2312 96-97 1.0498102500504025 2312 98-99 1.0893646328315434 2312 100 1.2636239000744744 2313 1 0.5029243478877845 2313 2 0.5547123639939784 2313 3 0.5422718964337179 2313 4 0.5057748118953995 2313 5 0.5084159524667555 2313 6 0.49857358413141384 2313 7 0.5488145922130414 2313 8 0.48246661371999977 2313 9 0.6381371040758665 2313 10-11 0.6366421456156175 2313 12-13 0.6342821358737183 2313 14-15 0.8163833220183889 2313 16-17 0.8245876904719864 2313 18-19 0.8139091472468536 2313 20-21 0.8595373403761997 2313 22-23 0.851176506957529 2313 24-25 0.7720450697241787 2313 26-27 0.7942493193612279 2313 28-29 0.8661378510834368 2313 30-31 0.8525970683468813 2313 32-33 0.9266440155862412 2313 34-35 0.9444439501233219 2313 36-37 0.9955067636154027 2313 38-39 1.0257923834336609 2313 40-41 1.0081947615217786 2313 42-43 0.8729158770306853 2313 44-45 0.9629192889348417 2313 46-47 0.993406951073176 2313 48-49 0.9631682982763365 2313 50-51 0.9645923917429897 2313 52-53 0.9600964640846641 2313 54-55 0.8211176038787613 2313 56-57 0.8387782235191068 2313 58-59 0.838046692069824 2313 60-61 0.7546412903659601 2313 62-63 0.7416841542201809 2313 64-65 0.814111611710068 2313 66-67 0.779413418628522 2313 68-69 0.8534726578080267 2313 70-71 0.8199841106257892 2313 72-73 0.8221719545492974 2313 74-75 0.8033759377905554 2313 76-77 0.8002419371717551 2313 78-79 0.8134230367603834 2313 80-81 0.8113013005551863 2313 82-83 0.7882221793280877 2313 84-85 0.8601713586396365 2313 86-87 0.8735607888782226 2313 88-89 0.892855355737229 2313 90-91 0.9628032057212543 2313 92-93 0.9626791319950492 2313 94-95 1.0246476385387133 2313 96-97 1.0943252798029128 2313 98-99 1.117674988082019 2313 100 1.1753500709469797 2314 1 0.4000915864132111 2314 2 0.3348846264084635 2314 3 0.4510559576011737 2314 4 0.35446377076736013 2314 5 0.2913315840206181 2314 6 0.3024446107018477 2314 7 0.3792084241817335 2314 8 0.4006821834358689 2314 9 0.4954398605850798 2314 10-11 0.4744237065849646 2314 12-13 0.4904987456465122 2314 14-15 0.5756543225475781 2314 16-17 0.5895229198519232 2314 18-19 0.638020808427207 2314 20-21 0.5991884156126943 2314 22-23 0.632843552562754 2314 24-25 0.6004438001539256 2314 26-27 0.707770139015274 2314 28-29 0.6669404509071981 2314 30-31 0.6641766209551676 2314 32-33 0.6854931798574952 2314 34-35 0.761597881335426 2314 36-37 0.7768353527176615 2314 38-39 0.8127673627380503 2314 40-41 0.8085380360526102 2314 42-43 0.656237855480164 2314 44-45 0.7873118889218631 2314 46-47 0.6939443005446435 2314 48-49 0.6757464767512147 2314 50-51 0.860022392766318 2314 52-53 0.8410534279466688 2314 54-55 0.8047344177607343 2314 56-57 0.7840038366330617 2314 58-59 0.8394896914156078 2314 60-61 0.6550175345420186 2314 62-63 0.6616122342042203 2314 64-65 0.7497328247302093 2314 66-67 0.8111398733046258 2314 68-69 0.8342880672149704 2314 70-71 0.7423498937086137 2314 72-73 0.7424502971954894 2314 74-75 0.7818606759904654 2314 76-77 0.7362929302230441 2314 78-79 0.7908123107154381 2314 80-81 0.8236604578542384 2314 82-83 0.7971306531241851 2314 84-85 0.7801912001543947 2314 86-87 0.7437961873669572 2314 88-89 0.7330736243450673 2314 90-91 0.770780068038345 2314 92-93 0.892272812972319 2314 94-95 1.0396823542213163 2314 96-97 1.014155764858863 2314 98-99 1.0756135649958587 2314 100 1.0377764218164742 2315 1 0.26570110078387543 2315 2 0.319423774079425 2315 3 0.3236030339143525 2315 4 0.24544494024208774 2315 5 0.26377955230604755 2315 6 0.2251403490566517 2315 7 0.24937690782891053 2315 8 0.23477534498195496 2315 9 0.14915641162571802 2315 10-11 0.28968634285850925 2315 12-13 0.27008249192996203 2315 14-15 0.4031072462343914 2315 16-17 0.25602440647233493 2315 18-19 0.34773339514673296 2315 20-21 0.2990893075849428 2315 22-23 0.32025683303846364 2315 24-25 0.4173912728992164 2315 26-27 0.5180773739012992 2315 28-29 0.4466233052184023 2315 30-31 0.4940081373079863 2315 32-33 0.528208739817849 2315 34-35 0.4338476335653567 2315 36-37 0.6349126569793029 2315 38-39 0.666087481667681 2315 40-41 0.6879235662805598 2315 42-43 0.6575262598409282 2315 44-45 0.5729412248980736 2315 46-47 0.6712485621898381 2315 48-49 0.4234174380990865 2315 50-51 0.4548687753233125 2315 52-53 0.26424470644023046 2315 54-55 0.39105750993119415 2315 56-57 0.33524268697994586 2315 58-59 0.42125381430955855 2315 60-61 0.5329164443537096 2315 62-63 0.4403040082379164 2315 64-65 0.5164325274061241 2315 66-67 0.4481071676554649 2315 68-69 0.5675883645390485 2315 70-71 0.455729477454895 2315 72-73 0.4941846876811198 2315 74-75 0.1941500714315012 2315 76-77 0.46572801446487233 2315 78-79 0.592001607048438 2315 80-81 0.5478428166252911 2315 82-83 0.5340979474750256 2315 84-85 0.502589614425947 2315 86-87 0.5330925203699302 2315 88-89 0.502151919686991 2315 90-91 0.5710773921215981 2315 92-93 0.7167524958266291 2315 94-95 0.7107428101182443 2315 96-97 0.7307068054931527 2315 98-99 0.889191364797643 2315 100 0.7251897022921945 2316 1 0.090618642273526 2316 2 0.07693874286355395 2316 3 0.1754699103702002 2316 4 0.024940270486446536 2316 5 0.0011203166372624196 2316 6 -0.14664771442298985 2316 7 -0.12456067936406612 2316 8 -0.041132684977711165 2316 9 0.017172215252941214 2316 10-11 0.06097985254521632 2316 12-13 -0.038458296231361544 2316 14-15 -0.13282608152280062 2316 16-17 0.09486652722631561 2316 18-19 0.16597027832550992 2316 20-21 0.2054763110190052 2316 22-23 0.10897035402475552 2316 24-25 0.08814270665150303 2316 26-27 -0.011776453593917324 2316 28-29 -0.014098924806908997 2316 30-31 -0.5709274266924353 2316 32-33 -0.44571158632330565 2316 34-35 -0.527446460038071 2316 36-37 -0.27105872005414966 2316 38-39 -0.12794940687681589 2316 40-41 -0.006107739546614255 2316 42-43 0.02138986320680658 2316 44-45 -0.16138913309022485 2316 46-47 -0.19258030207635102 2316 48-49 -0.18566055344832222 2316 50-51 -0.015767729414314147 2316 52-53 -0.339928542440866 2316 54-55 -0.23305830995101218 2316 56-57 -0.22561861780565806 2316 58-59 -0.5656146087365315 2316 60-61 -0.0409330424308294 2316 62-63 -0.06749682329106577 2316 64-65 -0.4631824071320736 2316 66-67 -0.715621582510245 2316 68-69 -0.7311767502242574 2316 70-71 -0.4755037349392879 2316 72-73 -0.6459274519475713 2316 74-75 -0.49534556260737617 2316 76-77 -0.1895091859227307 2316 78-79 -0.06904045547167925 2316 80-81 -0.17259632031422 2316 82-83 -0.059427343220811224 2316 84-85 -0.004193110950126311 2316 86-87 0.11181575883520622 2316 88-89 0.10524024734949222 2316 90-91 -0.555509815841134 2316 92-93 -0.5005932633598 2316 94-95 -0.034746058377397304 2316 96-97 0.1481818746162702 2316 98-99 0.12016692393735795 2316 100 -0.09904244721573718 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 134.0 1 304.0 2 198.0 3 185.0 4 187.0 5 158.0 6 190.0 7 397.0 8 1131.0 9 2090.0 10 3255.0 11 5017.0 12 7511.0 13 9724.0 14 11799.0 15 13375.0 16 16010.0 17 19794.0 18 23623.0 19 28409.0 20 34459.0 21 41203.0 22 46492.0 23 46975.0 24 46671.0 25 47171.0 26 51587.0 27 58792.0 28 68180.0 29 80531.0 30 98718.0 31 121663.0 32 153809.0 33 200205.0 34 279762.0 35 385200.0 36 567112.0 37 843505.0 38 816133.0 39 197084.0 40 173.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.25032643327371 19.319581583132635 13.441125439798219 22.988966543795435 2 15.750859840853124 20.299251443552073 41.042183542488814 22.90770517310599 3 18.791872498509186 26.814156286411183 29.63109804631011 24.76287316876952 4 12.641720644756587 16.878940053995418 38.48426981998062 31.99506948126738 5 13.099119190067054 37.691165237466564 34.58529475779228 14.624420814674096 6 29.84577738427295 37.049457964047 18.9036006385132 14.201164013166851 7 27.092931644654538 32.641110920830066 22.715535461260895 17.550421973254497 8 25.867368939671596 35.17210110180016 20.585346644845426 18.375183313682815 9 26.91574812247256 16.30324667822068 20.969543616406703 35.81146158290006 10-11 24.876981932084156 26.36457994761875 28.30996797896338 20.448470141333715 12-13 25.55780661194343 24.205408805525597 28.243133039127173 21.993651543403807 14-15 23.134276106662576 25.517167354205046 26.303472939210394 25.045083599921984 16-17 22.049362428255343 28.193084342358297 27.185166706203045 22.57238652318332 18-19 21.75014449040355 27.371589570069123 29.380451031907594 21.497814907619734 20-21 22.8445312205689 26.697698529851905 29.102858596903097 21.354911652676098 22-23 22.746908030638426 26.51219569691975 28.819010301711128 21.9218859707307 24-25 22.284049094574197 26.566457665794395 29.163348114686844 21.986145124944564 26-27 22.066783848344457 26.742975369080103 28.98660885940175 22.203631923173692 28-29 21.85840854206822 26.956242782428703 29.21746821841825 21.967880457084828 30-31 22.566055475459365 26.670298344574277 29.09460513245301 21.66904104751335 32-33 22.525296633921567 26.821509594908356 28.801410951700234 21.851782819469847 34-35 22.553223401992177 26.97220123950378 28.859559689931082 21.615015668572966 36-37 22.450774385174512 26.87539178568767 28.65026977965251 22.023564049485316 38-39 22.35465928527897 27.027268132359307 28.78136845177827 21.83670413058345 40-41 22.384817803705754 26.496337121216502 29.181650820579613 21.937194254498134 42-43 22.232620696294305 26.744592815953904 28.645880222291076 22.37690626546072 44-45 22.048806398297312 27.114371656309373 28.711022033990176 22.125799911403142 46-47 22.111390463440042 26.80088445561854 28.353004211242137 22.73472086969928 48-49 22.316111737447855 26.86820905380518 28.32966488126986 22.4860143274771 50-51 22.307489533458092 27.041786080259104 28.215337738177602 22.435386648105194 52-53 22.141477711991712 27.35522939524988 27.95176534210868 22.55152755064973 54-55 22.15042405934404 27.126577582809734 28.14327993925996 22.57971841858627 56-57 22.361082973639174 27.092762436130187 27.856790623963867 22.689363966266775 58-59 22.106095594389156 27.06464109633748 28.248181981444343 22.581081327829025 60-61 22.382119875397702 27.31894739810194 28.024203520167447 22.274729206332918 62-63 22.3559794222142 27.166569627079063 28.0277795947821 22.449671355924643 64-65 22.101524644771914 26.90894937173467 28.020860202527853 22.968665780965562 66-67 22.182445043760097 26.892134623445212 27.82211396173186 23.103306371062832 68-69 21.830393413408252 27.085520985862267 28.136414750026873 22.94767085070261 70-71 22.126808174347055 26.701709004515532 28.20022141370303 22.971261407434383 72-73 22.165318411570038 27.156085154462023 27.807348188679743 22.871248245288196 74-75 22.247556427319292 27.28654137753026 27.657074350781464 22.808827844368988 76-77 22.17630974971051 27.09771436627922 27.69022486530963 23.03575101870064 78-79 22.074801965630815 27.017218339754372 27.75095506551679 23.15702462909802 80-81 22.055965367453027 27.37980871472346 27.547037704132865 23.017188213690655 82-83 22.494653677940217 27.131349353327604 27.804064302632163 22.569932666100012 84-85 22.44945768600986 27.314796836817624 27.422776540377914 22.8129689367946 86-87 22.047963357869595 27.481690260647106 27.742473741362883 22.727872640120413 88-89 22.26592284303456 27.010728438840687 27.708424279977084 23.01492443814767 90-91 22.5800381480246 26.991391843461333 27.663016227589885 22.765553780924186 92-93 22.541105071131966 27.149367189273587 27.529439696764996 22.780088042829448 94-95 22.23413742660484 26.988912771957285 27.62749040711737 23.149459394320505 96-97 22.398695317919163 26.974584059765334 27.523611183194024 23.103109439121482 98-99 22.312479610877865 27.048894357585723 27.36397637763417 23.274649653902237 100 22.561939332033866 26.98862420700099 27.10619635033677 23.343240110628376 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1772.0 1 1265.0 2 1916.5 3 2639.5 4 1681.0 5 1217.5 6 1674.5 7 2858.5 8 4608.0 9 5429.0 10 4588.0 11 3738.5 12 3761.0 13 4173.5 14 4615.0 15 4988.0 16 5335.5 17 5733.0 18 6154.5 19 6569.0 20 7054.5 21 7678.0 22 8613.5 23 9957.0 24 11597.0 25 13856.5 26 16858.0 27 20525.5 28 24777.5 29 30902.5 30 39534.5 31 48420.0 32 59832.0 33 73912.5 34 87782.0 35 100547.0 36 115708.5 37 132590.5 38 141077.0 39 143571.5 40 142218.0 41 139233.5 42 139570.5 43 144064.0 44 150006.5 45 158578.5 46 165970.0 47 168300.5 48 171209.0 49 173451.5 50 172551.5 51 167950.5 52 159980.5 53 152166.0 54 144611.5 55 132524.5 56 119317.5 57 105067.5 58 90105.5 59 76683.5 60 62836.5 61 50350.0 62 40392.5 63 32056.5 64 24395.0 65 17752.5 66 13195.0 67 10285.0 68 8119.0 69 6321.5 70 5096.5 71 3896.0 72 2933.5 73 2389.0 74 1908.0 75 1458.5 76 1056.0 77 700.5 78 459.5 79 304.0 80 204.5 81 147.0 82 116.0 83 89.5 84 61.0 85 45.5 86 31.0 87 24.5 88 23.5 89 18.5 90 15.0 91 10.0 92 6.5 93 6.0 94 6.5 95 6.0 96 5.5 97 4.5 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02492540857803663 2 0.11226829072220389 3 0.017256052092486894 4 0.01781046340469531 5 0.019727802526082742 6 0.045946837499272336 7 0.04580823467122023 8 0.03774617017285621 9 0.03271026742029644 10-11 0.037064706268266696 12-13 0.03615223765025701 14-15 0.03234065987882417 16-17 0.03875104067623396 18-19 0.03786167252956629 20-21 0.04788727709200179 22-23 0.0476331719072396 24-25 0.06600959685981433 26-27 0.059726268654785634 28-29 0.05164110368507959 30-31 0.05022042469754553 32-33 0.044064149084897926 34-35 0.04487266558186853 36-37 0.04096868592506761 38-39 0.046131641270008475 40-41 0.05546423169218345 42-43 0.05605329371140489 44-45 0.059541464884049496 46-47 0.06096214387158355 48-49 0.06037308185236212 50-51 0.05524477721443428 52-53 0.06598649638847232 54-55 0.052692175131141376 56-57 0.056353599838851104 58-59 0.05463261472387083 60-61 0.0505900322390178 62-63 0.057820479769069205 64-65 0.055441131220841426 66-67 0.05346604092109896 68-69 0.05892930239348603 70-71 0.06065028750846632 72-73 0.06253297592284074 74-75 0.05337363903573088 76-77 0.05373169634153215 78-79 0.06405760703141386 80-81 0.06495852541375254 82-83 0.05413595459001745 84-85 0.06098524434292557 86-87 0.05844419249530368 88-89 0.0667719124141009 90-91 0.07241997765722412 92-93 0.07609295260060486 94-95 0.07954647306623644 96-97 0.07541148869601536 98-99 0.08531004066606974 100 0.1296629456427429 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4328916.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.81782231599726 #Duplication Level Percentage of deduplicated Percentage of total 1 78.3831237567831 50.022402644832084 2 13.68905521100004 17.472113862589538 3 3.949462659836926 7.561383188074168 4 1.531261796493023 3.908871729914661 5 0.7617997172601395 2.430819949824227 6 0.42807495031213827 1.639128667016964 7 0.27096145187179665 1.2104518853027275 8 0.1944872322434033 0.9929401304031686 9 0.13131683756137524 0.7542319145931466 >10 0.5933679646800992 6.539127943985331 >50 0.037393119056370085 1.6419150143650671 >100 0.0268800638109128 3.224880034961866 >500 0.0022521912887120818 0.9288271129647765 >1k 4.331136939647247E-4 0.5887952206201335 >5k 4.3311369396472353E-5 0.21777849700807556 >10k+ 8.662273879294471E-5 0.8663322035441188 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17714 0.40920174935249376 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13629 0.31483632392035327 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7879 0.182008613703754 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 2.310047134201726E-5 0.0 0.0 0.0 3 0.0 2.310047134201726E-5 0.0 2.310047134201726E-5 0.0 4 0.0 2.310047134201726E-5 0.0 2.310047134201726E-5 0.0 5 0.0 2.310047134201726E-5 0.0 2.310047134201726E-5 0.0 6 0.0 2.310047134201726E-5 0.0 2.310047134201726E-5 4.620094268403452E-5 7 0.0 2.310047134201726E-5 0.0 2.310047134201726E-5 4.620094268403452E-5 8 0.0 2.310047134201726E-5 0.0 2.310047134201726E-5 6.930141402605178E-5 9 0.0 2.310047134201726E-5 0.0 2.310047134201726E-5 6.930141402605178E-5 10-11 0.0 2.310047134201726E-5 0.0 3.465070701302589E-5 9.240188536806905E-5 12-13 0.0 2.310047134201726E-5 0.0 5.7751178355043154E-5 9.240188536806905E-5 14-15 0.0 2.310047134201726E-5 0.0 8.085164969706042E-5 1.1550235671008631E-4 16-17 0.0 2.310047134201726E-5 0.0 1.0395212103907767E-4 1.3860282805210357E-4 18-19 0.0 2.310047134201726E-5 0.0 1.2705259238109494E-4 1.848037707361381E-4 20-21 0.0 2.310047134201726E-5 0.0 2.0790424207815534E-4 1.9635400640714672E-4 22-23 0.0 2.310047134201726E-5 0.0 4.966601338533712E-4 2.4255494909118127E-4 24-25 0.0 4.620094268403452E-5 0.0 0.0012358752167979234 2.8875589177521576E-4 26-27 0.0 6.930141402605178E-5 0.0 0.0022984968985307174 3.0030612744622444E-4 28-29 0.0 6.930141402605178E-5 0.0 0.005290007937321953 3.0030612744622444E-4 30-31 0.0 6.930141402605178E-5 0.0 0.016736291487291506 3.349568344592503E-4 32-33 0.0 6.930141402605178E-5 0.0 0.043694541543425655 3.4650707013025894E-4 34-35 0.0 9.240188536806905E-5 0.0 0.08204132397117431 3.4650707013025894E-4 36-37 0.0 1.0395212103907767E-4 0.0 0.14643388783704744 3.4650707013025894E-4 38-39 0.0 1.2705259238109494E-4 0.0 0.24049900714174172 3.5805730580126756E-4 40-41 0.0 1.3860282805210357E-4 0.0 0.3482858064235943 4.1580848415631073E-4 42-43 0.0 1.6170329939412084E-4 0.0 0.45416681681973037 4.38908955498328E-4 44-45 0.0 1.6170329939412084E-4 0.0 0.5814619641499166 4.7355966251135385E-4 46-47 0.0 1.6170329939412084E-4 0.0 0.7340521275996116 4.966601338533712E-4 48-49 0.0 1.6170329939412084E-4 0.0 0.8950624128534719 5.31310840866397E-4 50-51 0.0 1.6170329939412084E-4 0.0 1.0656016425359143 5.31310840866397E-4 52-53 0.0 1.6170329939412084E-4 0.0 1.2444339414301409 5.775117835504315E-4 54-55 0.0 1.6170329939412084E-4 0.0 1.4471059267493294 6.237127262344661E-4 56-57 0.0 1.6170329939412084E-4 0.0 1.6655439837594446 6.237127262344661E-4 58-59 0.0 1.6170329939412084E-4 0.0 1.920180479362501 6.237127262344661E-4 60-61 0.0 1.6170329939412084E-4 0.0 2.174528219073782 6.237127262344661E-4 62-63 0.0 1.6170329939412084E-4 0.0 2.4387514102837757 6.237127262344661E-4 64-65 0.0 1.6170329939412084E-4 0.0 2.70298615172944 6.237127262344661E-4 66-67 0.0 1.848037707361381E-4 0.0 2.9993536488118506 6.237127262344661E-4 68-69 0.0 1.848037707361381E-4 0.0 3.3340448278506676 6.237127262344661E-4 70-71 0.0 1.848037707361381E-4 0.0 3.677560386942135 6.814639045895092E-4 72-73 2.310047134201726E-5 1.848037707361381E-4 0.0 4.008047280196705 8.316169683126215E-4 74-75 4.620094268403452E-5 1.848037707361381E-4 0.0 4.340948172706517 8.316169683126215E-4 76-77 4.620094268403452E-5 2.3100471342017261E-4 0.0 4.711618335860525 8.4316720398363E-4 78-79 4.620094268403452E-5 2.4255494909118127E-4 0.0 5.111325791491449 8.77817910996656E-4 80-81 4.620094268403452E-5 2.541051847621899E-4 0.0 5.5194071679838554 9.240188536806906E-4 82-83 4.620094268403452E-5 2.541051847621899E-4 0.0 5.9426540039122955 9.702197963647251E-4 84-85 4.620094268403452E-5 2.541051847621899E-4 0.0 6.380303983722484 9.817700320357337E-4 86-87 4.620094268403452E-5 2.541051847621899E-4 0.0 6.8155861652201155 0.0010164207390487596 88 4.620094268403452E-5 2.541051847621899E-4 0.0 7.153084051526988 0.0010164207390487596 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7830 0.0 53.65379 1 GTATCAA 14655 0.0 41.218285 1 CAACGCA 17545 0.0 33.534714 5 ATCAACG 17510 0.0 33.521233 3 TACCTGG 3090 0.0 32.731476 2 TATCAAC 18245 0.0 32.56455 2 TCAACGC 18295 0.0 32.133907 4 AACGCAG 18620 0.0 31.826147 6 ACGCAGA 20880 0.0 28.08845 7 GTACATG 18450 0.0 27.869843 1 CGCAGAG 21365 0.0 27.363153 8 TACATGG 19435 0.0 26.383204 2 GTACCTG 4065 0.0 25.345114 1 ACCTGGG 3920 0.0 25.295311 3 ACATGGG 19700 0.0 25.143053 3 TGGTATC 3820 0.0 24.8756 2 GTGGTAT 3840 0.0 24.747475 1 GCAGAGT 23030 0.0 24.609743 9 CATGGGG 13310 0.0 22.737696 4 GTACTAG 1715 0.0 22.493677 1 >>END_MODULE