FastQCFastQC Report
Mon 6 Jun 2016
ERR1211292_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211292_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4328916
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150540.34775449558272786No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124230.2869771554818804No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73890.17068938274616555No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76450.052.1813431
GTATCAA141400.040.294261
ATCAACG172100.032.3913543
CAACGCA173150.032.1065835
TATCAAC181350.031.1772582
TCAACGC181000.030.8508384
AACGCAG185300.030.25356
TACCTGG32750.028.5108552
TGGTATC37850.027.5204522
GTGGTAT38900.027.0254061
ACGCAGA209050.026.5471387
CGCAGAG213100.026.0426068
GTACATG192550.025.9585231
CGTTAAC5150.025.5167081
TACATGG196750.024.9451082
ACATGGG196750.024.2787653
GTACCTG40750.023.8405691
GCAGAGT232250.023.6518029
ACCTGGG41100.022.4914763
CATGGGG132100.022.1656444
Produced by FastQC (version 0.11.5)