##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211292_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4328916 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1529172661239 33.0 31.0 34.0 30.0 34.0 2 32.28175021183132 34.0 31.0 34.0 30.0 34.0 3 32.344070432413105 34.0 31.0 34.0 30.0 34.0 4 35.974878930429696 37.0 35.0 37.0 35.0 37.0 5 35.88432138669357 37.0 35.0 37.0 35.0 37.0 6 35.90216626980057 37.0 35.0 37.0 35.0 37.0 7 35.824888725029545 37.0 35.0 37.0 35.0 37.0 8 35.809812433412894 37.0 35.0 37.0 35.0 37.0 9 37.435558694139594 39.0 37.0 39.0 34.0 39.0 10-11 37.381996324252995 39.0 37.0 39.0 34.0 39.0 12-13 37.31323961934119 39.0 37.0 39.0 34.0 39.0 14-15 38.54914371172829 40.0 38.0 41.0 34.0 41.0 16-17 38.52933390252895 40.0 38.0 41.0 34.0 41.0 18-19 38.5449171339892 40.0 38.0 41.0 34.0 41.0 20-21 38.5167149235513 40.0 38.0 41.0 34.0 41.0 22-23 38.406042066882335 40.0 38.0 41.0 34.0 41.0 24-25 38.31379426165812 40.0 38.0 41.0 34.0 41.0 26-27 38.214912694078606 40.0 38.0 41.0 34.0 41.0 28-29 38.11293566333927 40.0 37.5 41.0 33.0 41.0 30-31 38.11318272288028 40.0 37.0 41.0 33.0 41.0 32-33 38.036411540441065 40.0 37.0 41.0 33.0 41.0 34-35 37.900469424678136 40.0 37.0 41.0 33.0 41.0 36-37 37.75066691060765 40.0 37.0 41.0 33.0 41.0 38-39 37.64086055261872 40.0 37.0 41.0 33.0 41.0 40-41 37.53168056391023 40.0 36.5 41.0 32.5 41.0 42-43 37.42969591001535 40.0 36.0 41.0 32.0 41.0 44-45 37.27379660404591 40.0 36.0 41.0 31.5 41.0 46-47 37.03199126524977 39.5 36.0 41.0 31.0 41.0 48-49 35.49666163538401 39.0 35.0 41.0 24.0 41.0 50-51 36.22659633497162 39.0 35.0 40.5 29.0 41.0 52-53 36.323596831169745 39.0 35.0 40.0 30.0 41.0 54-55 36.165604737999075 39.0 35.0 40.0 29.0 41.0 56-57 35.95945555423113 38.0 35.0 40.0 28.5 41.0 58-59 35.73012227541491 38.0 34.5 40.0 28.0 41.0 60-61 35.481766566965035 38.0 34.0 40.0 28.0 41.0 62-63 35.1658762840397 37.0 34.0 40.0 27.5 41.0 64-65 34.83894362930582 37.0 34.0 39.5 27.0 41.0 66-67 34.51030835433166 36.0 33.5 39.0 26.0 41.0 68-69 34.1529524943427 36.0 33.0 39.0 26.0 40.0 70-71 33.793279772580476 35.0 33.0 38.5 26.0 40.0 72-73 33.413674924623166 35.0 32.5 37.5 26.0 40.0 74-75 33.04598183471336 35.0 32.0 37.0 25.0 39.0 76-77 32.098541297636636 34.0 30.5 36.0 24.5 38.5 78-79 32.528399372960806 35.0 32.0 36.0 25.0 38.5 80-81 32.39544876823667 35.0 32.0 36.0 25.0 37.5 82-83 32.12411837051123 34.5 31.5 35.0 25.0 37.0 84-85 31.837750258956284 34.0 31.0 35.0 24.0 36.5 86-87 31.577668289243775 34.0 31.0 35.0 24.0 36.0 88-89 31.34804613903342 34.0 31.0 35.0 23.0 36.0 90-91 31.132745010529195 34.0 31.0 35.0 23.0 35.0 92-93 30.941481423986975 34.0 30.5 35.0 21.5 35.0 94-95 30.750224883088514 34.0 30.0 35.0 20.0 35.0 96-97 30.56560637813254 34.0 30.0 35.0 20.0 35.0 98-99 30.351305269032707 34.0 29.0 35.0 19.5 35.0 100 27.90734424045188 31.0 25.0 34.0 14.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.26552724569743447 1101 2 -0.2513004241227179 1101 3 -0.29733715236479696 1101 4 -0.16289427059263062 1101 5 -0.12878118527963522 1101 6 -0.2791387509859433 1101 7 -0.20562419690713796 1101 8 -0.16807710128209408 1101 9 -0.29846572065591914 1101 10-11 -0.287428121354246 1101 12-13 -0.3310842567847132 1101 14-15 -0.4387364039886066 1101 16-17 -0.39974507397579373 1101 18-19 -0.3395330326765915 1101 20-21 -0.46246655357199273 1101 22-23 -0.5046387640628254 1101 24-25 -0.5031860817452198 1101 26-27 -0.6054271973470335 1101 28-29 -0.538333308776437 1101 30-31 -0.4670221416642306 1101 32-33 -0.41592714799111263 1101 34-35 -0.4928355400556583 1101 36-37 -0.5671069269019924 1101 38-39 -0.46950345342975197 1101 40-41 -0.512891443753702 1101 42-43 -0.5498543362399602 1101 44-45 -0.7034682568335526 1101 46-47 -0.5468249997174794 1101 48-49 0.688830897927339 1101 50-51 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0.790730155208287 2209 80-81 0.8364712650593944 2209 82-83 0.7504772796906494 2209 84-85 0.7901863640319924 2209 86-87 0.8187127420215887 2209 88-89 0.8933205001222255 2209 90-91 0.9428593719431007 2209 92-93 0.8725674930133991 2209 94-95 0.9812332524696323 2209 96-97 0.9759646813702822 2209 98-99 1.0896291059008263 2209 100 1.2751730497635805 2210 1 0.546875605462084 2210 2 0.5166555673876658 2210 3 0.4528448703324486 2210 4 0.3799534674989715 2210 5 0.35029123909659887 2210 6 0.4063308032136348 2210 7 0.4340342876135139 2210 8 0.45148684562187924 2210 9 0.5504256484890178 2210 10-11 0.5610073346533468 2210 12-13 0.589479055453296 2210 14-15 0.7355123618175767 2210 16-17 0.7705296074611283 2210 18-19 0.7493093548715777 2210 20-21 0.7609853815363721 2210 22-23 0.7687769222141227 2210 24-25 0.7526178834949349 2210 26-27 0.7895269694727531 2210 28-29 0.8016388963006023 2210 30-31 0.8679792399850967 2210 32-33 0.8176494428208301 2210 34-35 0.8298670864482958 2210 36-37 0.838337653804281 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2311 3 0.37040948367759086 2311 4 0.2796646735230368 2311 5 0.2972184358055685 2311 6 0.34434376215862983 2311 7 0.4420105566100361 2311 8 0.16150270461273664 2311 9 0.46862316535014514 2311 10-11 0.37725422018983323 2311 12-13 0.47805087175854766 2311 14-15 0.47153218686833753 2311 16-17 0.5035530246372417 2311 18-19 0.635226591308026 2311 20-21 0.655457609338967 2311 22-23 0.69337050223929 2311 24-25 0.6844201380570993 2311 26-27 0.6571334546850593 2311 28-29 0.7450190480704606 2311 30-31 0.568431745681103 2311 32-33 0.6561109158351854 2311 34-35 0.8518852249415687 2311 36-37 0.8354097766139787 2311 38-39 0.8511192796534033 2311 40-41 0.8120668771746793 2311 42-43 0.8333810329981972 2311 44-45 0.800029316462961 2311 46-47 0.8474487515980726 2311 48-49 -6.039450564833082 2311 50-51 -1.0787358097111195 2311 52-53 0.38039617916442836 2311 54-55 0.7981185739414727 2311 56-57 0.8408584421356196 2311 58-59 0.9428504330516105 2311 60-61 0.7881063773632704 2311 62-63 0.6689255008864663 2311 64-65 0.7659829158636597 2311 66-67 0.9008255611308726 2311 68-69 0.9699135810356267 2311 70-71 0.9502877864294916 2311 72-73 0.434047691391406 2311 74-75 0.7811750482488904 2311 76-77 0.9212688715457986 2311 78-79 0.688659385344792 2311 80-81 0.6923610450306086 2311 82-83 0.7454101321558824 2311 84-85 0.853410711142832 2311 86-87 0.873780680457827 2311 88-89 0.8812536214939648 2311 90-91 0.9395238557217098 2311 92-93 0.9398594147457899 2311 94-95 0.9703768959945229 2311 96-97 1.0351696672131432 2311 98-99 1.0001798580481243 2311 100 1.2406901253263065 2312 1 0.009701049869697442 2312 2 0.144501160970556 2312 3 0.15791740786487907 2312 4 -0.023249924589485715 2312 5 0.15238921378229975 2312 6 0.04059297600161216 2312 7 0.22179817461498885 2312 8 0.28741393918797087 2312 9 0.09034556995495535 2312 10-11 0.13734858908890146 2312 12-13 0.20448037510768557 2312 14-15 0.2606231952357305 2312 16-17 0.35319772298963414 2312 18-19 0.2339888276182478 2312 20-21 0.3087788926477941 2312 22-23 0.5071976862029217 2312 24-25 0.587883687296511 2312 26-27 0.5600946899741288 2312 28-29 0.6542463712443052 2312 30-31 0.6261171365980687 2312 32-33 0.6005886934209386 2312 34-35 0.2148466280443202 2312 36-37 0.5224138965652045 2312 38-39 0.613132109635842 2312 40-41 0.38400071450010387 2312 42-43 0.4341708092875294 2312 44-45 0.5467522533126683 2312 46-47 0.6447674520789448 2312 48-49 -4.74860195857584 2312 50-51 -1.065619604744981 2312 52-53 0.34064913199650704 2312 54-55 0.6319884727498177 2312 56-57 0.7853657749675094 2312 58-59 0.6609561897372913 2312 60-61 0.7157288982604584 2312 62-63 0.45823667079081076 2312 64-65 0.6072346757157661 2312 66-67 0.6078932474034389 2312 68-69 0.6243491346942989 2312 70-71 0.5851453063486005 2312 72-73 0.6920264807434009 2312 74-75 0.42996216857355307 2312 76-77 0.6410501977898733 2312 78-79 0.3157399306541109 2312 80-81 0.5561594501994662 2312 82-83 0.6041316723949777 2312 84-85 0.6295935909909893 2312 86-87 0.7004107963018598 2312 88-89 0.6837478713705529 2312 90-91 0.5421920181843944 2312 92-93 0.6914321671131525 2312 94-95 0.8678809304108519 2312 96-97 0.8358644283954852 2312 98-99 0.8594910004009684 2312 100 0.41594334240232556 2313 1 0.061461479479021364 2313 2 0.19244104489669667 2313 3 0.2943075366116261 2313 4 0.08186071488355395 2313 5 0.16703832272401087 2313 6 0.2577507438300728 2313 7 0.10557432520336363 2313 8 0.20226445400697202 2313 9 0.4788444086128152 2313 10-11 0.2647651700761031 2313 12-13 0.03875463291797843 2313 14-15 0.09064410575602722 2313 16-17 0.10894444584180718 2313 18-19 0.40271885647278083 2313 20-21 0.3797364163489121 2313 22-23 0.42871382697465066 2313 24-25 0.22710658065415856 2313 26-27 0.470641493799711 2313 28-29 0.3559346522997018 2313 30-31 0.5171782847404387 2313 32-33 0.46640706404462406 2313 34-35 0.39772184334405125 2313 36-37 -0.32040449216494693 2313 38-39 0.20445059795154918 2313 40-41 0.42185725485705206 2313 42-43 0.5156860333175146 2313 44-45 0.5850184320508873 2313 46-47 0.3618966736787499 2313 48-49 -5.377658275717586 2313 50-51 -1.3550318330032383 2313 52-53 0.027166397489644112 2313 54-55 0.33586229385173993 2313 56-57 0.24625315976920348 2313 58-59 0.3809996550406609 2313 60-61 0.6722565806802336 2313 62-63 0.7838960845948222 2313 64-65 0.8173986990832134 2313 66-67 0.8977860718113462 2313 68-69 0.9364973330113102 2313 70-71 0.8734813825667089 2313 72-73 0.6424038374180938 2313 74-75 0.7721015240845333 2313 76-77 0.8149659753230836 2313 78-79 0.7980896343524151 2313 80-81 -0.13818013443169264 2313 82-83 0.31564030469296256 2313 84-85 0.6477114745181289 2313 86-87 0.7436085399426986 2313 88-89 0.6581049980670315 2313 90-91 0.7605777224288275 2313 92-93 0.8165579948110526 2313 94-95 0.7223078061468371 2313 96-97 0.7351530074408892 2313 98-99 0.8278379633350745 2313 100 1.0137748566884106 2314 1 0.01687855544577843 2314 2 0.16355553437885817 2314 3 0.21097472755405278 2314 4 0.0937800778208171 2314 5 0.2290068414910209 2314 6 0.28720940057186795 2314 7 0.10989028271074375 2314 8 0.26325817822703357 2314 9 0.42818089450256025 2314 10-11 0.3299432718607207 2314 12-13 0.1894523709534397 2314 14-15 0.25638231323464566 2314 16-17 0.46837175354774274 2314 18-19 0.5544551383329832 2314 20-21 0.5840398509656808 2314 22-23 0.3148159468670926 2314 24-25 0.486919808410299 2314 26-27 0.5366767466326934 2314 28-29 0.6595372430448734 2314 30-31 0.5615847932988132 2314 32-33 0.6244059459436073 2314 34-35 0.4588810329756612 2314 36-37 0.5184345462841122 2314 38-39 0.5846630570530635 2314 40-41 0.40109888134362137 2314 42-43 0.42665823568582795 2314 44-45 0.6717568103504874 2314 46-47 0.7079352593128263 2314 48-49 -6.514646554371616 2314 50-51 -1.4488630849618076 2314 52-53 -0.7768473581790047 2314 54-55 -0.3520582532851009 2314 56-57 -0.06455459255708007 2314 58-59 0.5654495497408689 2314 60-61 0.7731230492370216 2314 62-63 0.5919916256892108 2314 64-65 0.7020177224888826 2314 66-67 0.8259968195175063 2314 68-69 0.8127610200927435 2314 70-71 0.5205005762358965 2314 72-73 0.7575118787065946 2314 74-75 0.7901291110768156 2314 76-77 0.7626236373213047 2314 78-79 0.5755984431806311 2314 80-81 0.6497411692549733 2314 82-83 0.6267584415416749 2314 84-85 0.7007711013957199 2314 86-87 0.7322209120808054 2314 88-89 0.6628251124573659 2314 90-91 0.8481204326531184 2314 92-93 0.8882446549660834 2314 94-95 0.8781123152634613 2314 96-97 0.8900817304922874 2314 98-99 1.021171684234286 2314 100 -0.43415337142012334 2315 1 0.0902184959809631 2315 2 0.13620171871978926 2315 3 0.2841164520337358 2315 4 0.07118344749375893 2315 5 0.1673616482303686 2315 6 0.2658020665778196 2315 7 0.2686613431666416 2315 8 0.22123637446092914 2315 9 0.39646632562248385 2315 10-11 0.22389768216795858 2315 12-13 0.3808299725391677 2315 14-15 0.17164494950818465 2315 16-17 0.3174302272841203 2315 18-19 0.40817168937361714 2315 20-21 0.305744509042988 2315 22-23 0.36645122932497287 2315 24-25 0.4062458738215966 2315 26-27 0.4187381936099044 2315 28-29 0.5045322876434781 2315 30-31 0.4645580341313149 2315 32-33 0.5087468775590693 2315 34-35 0.5745401013601921 2315 36-37 0.4141729010908577 2315 38-39 0.5057730669638687 2315 40-41 0.48940413406909755 2315 42-43 0.5777979780049591 2315 44-45 0.616553638893599 2315 46-47 0.5195308985596014 2315 48-49 -5.0698497258285045 2315 50-51 -1.035681717271018 2315 52-53 0.14901983706381117 2315 54-55 0.048239070798153705 2315 56-57 0.3885673103170504 2315 58-59 0.450484237550576 2315 60-61 0.4028554575621044 2315 62-63 0.25195198247811135 2315 64-65 0.5039998830438819 2315 66-67 -0.12028662944186408 2315 68-69 0.47906429065766076 2315 70-71 0.48492079427356316 2315 72-73 0.5233196091327628 2315 74-75 0.7004363459628351 2315 76-77 0.6220884539714504 2315 78-79 0.5768868475413882 2315 80-81 0.48571738332831416 2315 82-83 0.44360680625921844 2315 84-85 0.4027532619507248 2315 86-87 0.4584845394346182 2315 88-89 0.4656992452621225 2315 90-91 0.5391016021278467 2315 92-93 0.6172833070969084 2315 94-95 0.3150796096143047 2315 96-97 0.3293285094813818 2315 98-99 0.2725096426089131 2315 100 0.3872539625739293 2316 1 0.20400307331994583 2316 2 0.2425810878372232 2316 3 0.12821806459079 2316 4 -0.07040207579031232 2316 5 0.1219295735269057 2316 6 0.11176333553932949 2316 7 0.2176293098107962 2316 8 0.24834123643564254 2316 9 0.4541792737391148 2316 10-11 0.3684447679500593 2316 12-13 0.31643794426496896 2316 14-15 0.42499850556102814 2316 16-17 0.40799050327157005 2316 18-19 0.1293561881205605 2316 20-21 0.3640451614515712 2316 22-23 0.5083694470533118 2316 24-25 0.5778895560004642 2316 26-27 0.5485148691352677 2316 28-29 0.6228639981091604 2316 30-31 0.5531858022122194 2316 32-33 0.48746360614195083 2316 34-35 0.4933351773640666 2316 36-37 0.3651269736732772 2316 38-39 0.4963893082130042 2316 40-41 0.07551255793581646 2316 42-43 0.056792658839235344 2316 44-45 0.2689278324572584 2316 46-47 0.36263395433785917 2316 48-49 -4.734004143395243 2316 50-51 -0.9623270210024089 2316 52-53 0.20622639523769948 2316 54-55 0.45290430897305356 2316 56-57 0.31219141708228193 2316 58-59 0.19922972403211503 2316 60-61 0.43269626529252747 2316 62-63 0.38472028432202876 2316 64-65 0.3946904947676728 2316 66-67 0.4003910848240153 2316 68-69 -4.4559805097321714E-4 2316 70-71 -0.4490711287378133 2316 72-73 -0.19378358789020922 2316 74-75 0.11872775433841554 2316 76-77 0.08025650204831436 2316 78-79 0.19621139398012843 2316 80-81 0.17032529902967752 2316 82-83 0.06279630222861954 2316 84-85 0.23516645957339577 2316 86-87 0.23056896037860497 2316 88-89 0.08126011305706626 2316 90-91 0.12170709773111099 2316 92-93 0.1436189179684888 2316 94-95 0.4055195908658398 2316 96-97 0.4866274488045441 2316 98-99 0.5385785179537415 2316 100 -0.31305547383596277 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 4.0 11 8.0 12 20.0 13 99.0 14 382.0 15 1100.0 16 2657.0 17 5600.0 18 9686.0 19 13884.0 20 19148.0 21 26633.0 22 33291.0 23 35343.0 24 36181.0 25 38784.0 26 45266.0 27 54808.0 28 67291.0 29 82404.0 30 101770.0 31 126545.0 32 159203.0 33 208251.0 34 292298.0 35 410240.0 36 635097.0 37 972717.0 38 834508.0 39 115686.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.478638443084606 19.968509351761263 14.131878989766694 23.420973215387438 2 15.47108763744787 20.66037350106484 40.58066130215997 23.287877559327328 3 18.502709225782368 26.586772842691186 30.16436924566727 24.746148685859172 4 12.693667272261383 17.19117268876429 38.32589683355526 31.789263205419065 5 13.035721463417113 37.84344318001738 34.58909251857639 14.531742837989118 6 30.138274865243336 37.23880862498077 18.500875739273177 14.122040770502716 7 27.22664391115185 32.64862734625821 22.505020889765888 17.619707852824057 8 25.9809644816633 35.43346231591106 20.344371931850997 18.24120127057465 9 26.836994789612678 16.141943028816886 20.80996768141345 36.21109450015698 10-11 24.9454764449695 26.430161915496054 28.198478445725815 20.425883193808627 12-13 25.42158692779929 24.154628709780738 28.29448003268258 22.129304329737394 14-15 22.98598089011963 25.581794503815914 26.19463895377881 25.23758565228565 16-17 22.016436046108712 28.288433551248588 27.1008685809328 22.594261821709903 18-19 21.726206220265354 27.364524366960314 29.31102828306862 21.598241129705713 20-21 22.716175038017646 26.777623748790404 29.042789283494162 21.46341192969779 22-23 22.63731036925838 26.539866361169963 28.803290161152706 22.01953310841895 24-25 22.26369786869104 26.587324183614246 29.0998489805465 22.049128967148217 26-27 22.03750296119475 26.790025621914427 28.9541608785488 22.218310538342028 28-29 21.89341163863028 26.991834009279792 29.14611520308367 21.968639149006258 30-31 22.582196662119596 26.64747348858054 29.075712756090322 21.69461709320954 32-33 22.512061055828266 26.822090113255236 28.796354855235563 21.869493975680935 34-35 22.5478641357238 26.96070593823965 28.852986679556125 21.638443246480428 36-37 22.455526510454675 26.83239741923618 28.698480005573085 22.013596064736056 38-39 22.31845692676724 26.99934530363667 28.822525066945502 21.85967270265059 40-41 22.34104460871817 26.51297172835248 29.210235538153288 21.935748124776058 42-43 22.216039930953418 26.73497784077888 28.651316154238526 22.39766607402917 44-45 21.97868929323136 27.13262145165689 28.750937251320124 22.13775200379163 46-47 22.07543636742321 26.765693788421597 28.374634230640517 22.784235613514674 48-49 22.376472659300628 26.93560738140612 28.242468860050916 22.445451099242337 50-51 22.266033036827654 27.03140353953798 28.181405653639874 22.521157769994492 52-53 22.050944718007866 27.323937446396357 28.010214266702153 22.61490356889362 54-55 22.10229625680742 27.10642301558927 28.154001743115188 22.637278984488123 56-57 22.351141362945075 26.998976332275475 27.884075810091737 22.765806494687716 58-59 22.000110198654443 27.052857119589678 28.27781796961174 22.669214712144132 60-61 22.320710250410606 27.3536066096969 28.00924671905612 22.316436420836375 62-63 22.319752518701616 27.14504179729585 28.00571874842254 22.529486935579985 64-65 21.94704114265144 26.922158048945494 28.000359978271916 23.130440830131153 66-67 22.029671798135276 26.911117935507605 27.815750999801047 23.243459266556073 68-69 21.75626305495133 27.00136252705153 28.09177699384685 23.150597424150295 70-71 21.93518005463357 26.66828336809357 28.19611242999236 23.2004241472805 72-73 21.979355774276303 27.048335067433506 27.79851535146548 23.17379380682471 74-75 22.01989904813413 27.16504349025418 27.64173303975963 23.173324421852058 76-77 21.854008262663186 26.976233320252653 27.752826160964478 23.416932256119686 78-79 21.736858368107196 26.856410182364236 27.830474897844258 23.576256551684306 80-81 21.684065864572133 27.218300996941064 27.566342707889717 23.531290430597085 82-83 22.055958491762638 26.922282953054864 27.85494758620451 23.166810968977988 84-85 22.00308213049411 27.05771606482248 27.489188917223455 23.450012887459952 86-87 21.533500682319897 27.229208190056937 27.806534466512716 23.430756661110447 88-89 21.665086258840184 26.801806459872218 27.78756730694462 23.745539974342975 90-91 21.897771091589107 26.656570378001998 27.81878344073107 23.626875089677828 92-93 21.822642304114343 26.834217839551442 27.672039164482115 23.671100691852097 94-95 21.38189458655727 26.66021349748101 27.829673296758955 24.12821861920276 96-97 21.46808374733772 26.57869456103869 27.797069298275705 24.156152393347885 98-99 21.242136648316198 26.653150741267645 27.75462934885008 24.35008326156608 100 21.218770082503344 26.547576049789935 27.763762631251904 24.469891236454817 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1670.0 1 1192.5 2 1643.0 3 2297.5 4 1497.0 5 1043.5 6 1458.5 7 2618.5 8 4066.5 9 4710.0 10 4188.0 11 3409.5 12 3387.0 13 3726.0 14 4129.5 15 4641.5 16 5068.5 17 5431.5 18 5859.0 19 6344.0 20 6835.5 21 7558.5 22 8424.5 23 9633.5 24 11401.5 25 13561.5 26 16715.0 27 20586.5 28 24740.5 29 30821.0 30 39366.5 31 48206.5 32 59999.5 33 74174.5 34 87227.5 35 99929.5 36 115478.5 37 132401.5 38 140810.5 39 143517.0 40 142093.5 41 138365.0 42 138725.0 43 143261.5 44 149937.0 45 159623.5 46 166962.5 47 169043.0 48 172021.0 49 174480.5 50 173844.5 51 170121.5 52 163784.0 53 156306.0 54 147651.5 55 134783.0 56 120760.0 57 106083.5 58 91128.5 59 77378.5 60 62591.0 61 49369.5 62 39353.5 63 30858.5 64 23028.0 65 16485.0 66 12164.0 67 9401.5 68 7321.5 69 5600.5 70 4374.0 71 3388.0 72 2644.5 73 2082.0 74 1597.0 75 1186.5 76 823.5 77 512.5 78 311.5 79 191.0 80 121.5 81 79.0 82 58.5 83 31.5 84 16.5 85 12.5 86 6.0 87 2.5 88 4.0 89 5.0 90 2.0 91 0.0 92 0.5 93 1.0 94 0.5 95 0.5 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029614804260466133 2 0.0021483438348076055 3 9.240188536806905E-5 4 0.02169134259015421 5 7.854160256285869E-4 6 4.620094268403452E-5 7 4.620094268403452E-5 8 0.003834678242774866 9 0.010325910689881717 10-11 0.001224324981126915 12-13 1.0395212103907768E-4 14-15 8.085164969706042E-5 16-17 3.696075414722762E-4 18-19 2.310047134201726E-5 20-21 1.1550235671008632E-4 22-23 2.19454477749164E-4 24-25 8.085164969706042E-5 26-27 1.0395212103907768E-4 28-29 0.00109727238874582 30-31 0.002795157032384089 32-33 0.009667547256634223 34-35 0.01921959215655836 36-37 0.02246520838011179 38-39 0.022072500367297493 40-41 0.005382409822690022 42-43 0.0056365150074522115 44-45 0.0100371547981065 46-47 0.012462704289018314 48-49 1.189501020578824 50-51 0.019335094513268446 52-53 0.019681601583398708 54-55 0.010591566110314914 56-57 0.008385471097152266 58-59 0.008535624160875378 60-61 0.005671165714465238 62-63 0.008004313320008982 64-65 0.02025911336694914 66-67 0.030146115101332527 68-69 0.029926660623583363 70-71 0.027119953355528265 72-73 0.02363178218288366 74-75 0.009644446785292207 76-77 0.015026856607982229 78-79 0.008166016619403102 80-81 0.002876008682081149 82-83 0.014287641525037677 84-85 0.02461355221491939 86-87 0.02388588736764585 88-89 0.03341483179622797 90-91 0.021321735048681935 92-93 0.014010435868933468 94-95 0.006779988338882066 96-97 0.003638324236367719 98-99 0.015904674518978885 100 0.03361118580263512 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4328916.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.09639479942936 #Duplication Level Percentage of deduplicated Percentage of total 1 78.54699962447728 50.34579498241126 2 13.483158740094279 17.28443731496919 3 3.9599376572807916 7.614531823865909 4 1.56725448525621 4.01821448952634 5 0.7689657991170047 2.464396772373111 6 0.43544485651595854 1.6746267261976648 7 0.26948885371243675 1.209128477311861 8 0.18335056686502035 0.9401688256383613 9 0.13078522701875672 0.7544575390434505 >10 0.5894432182827797 6.533132930411088 >50 0.03617834305503989 1.6109909635164603 >100 0.02657085769455408 3.2603857789980206 >500 0.0019028197891919388 0.8087037343306183 >1k 3.89213130505524E-4 0.5145767221237869 >5k 4.3245903389502667E-5 0.20481617107157196 >10k+ 8.649180677900533E-5 0.7616367482113388 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15054 0.34775449558272786 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12423 0.2869771554818804 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7389 0.17068938274616555 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.310047134201726E-5 0.0 4 0.0 0.0 0.0 2.310047134201726E-5 0.0 5 0.0 0.0 0.0 2.310047134201726E-5 0.0 6 0.0 0.0 0.0 2.310047134201726E-5 2.310047134201726E-5 7 0.0 0.0 0.0 2.310047134201726E-5 2.310047134201726E-5 8 0.0 2.310047134201726E-5 0.0 2.310047134201726E-5 2.310047134201726E-5 9 0.0 2.310047134201726E-5 0.0 4.620094268403452E-5 4.620094268403452E-5 10-11 0.0 2.310047134201726E-5 0.0 4.620094268403452E-5 4.620094268403452E-5 12-13 0.0 2.310047134201726E-5 0.0 4.620094268403452E-5 4.620094268403452E-5 14-15 0.0 3.465070701302589E-5 0.0 5.7751178355043154E-5 1.0395212103907767E-4 16-17 0.0 4.620094268403452E-5 0.0 8.085164969706042E-5 1.3860282805210357E-4 18-19 0.0 4.620094268403452E-5 0.0 1.0395212103907767E-4 1.6170329939412084E-4 20-21 0.0 4.620094268403452E-5 0.0 1.848037707361381E-4 1.848037707361381E-4 22-23 0.0 4.620094268403452E-5 0.0 4.735596625113539E-4 2.3100471342017261E-4 24-25 0.0 5.7751178355043154E-5 0.0 0.0012358752167979234 3.1185636311723306E-4 26-27 0.0 6.930141402605178E-5 0.0 0.0023215973698727348 3.5805730580126756E-4 28-29 0.0 6.930141402605178E-5 0.0 0.005301558172992962 3.696075414722762E-4 30-31 0.0 6.930141402605178E-5 0.0 0.0167247412516205 3.8115777714328486E-4 32-33 0.0 9.240188536806905E-5 0.0 0.04381004390013574 4.1580848415631073E-4 34-35 0.0 9.240188536806905E-5 0.0 0.08200667326416128 4.38908955498328E-4 36-37 0.0 9.240188536806905E-5 0.0 0.1456369215757478 4.38908955498328E-4 38-39 0.0 9.240188536806905E-5 0.0 0.23595976452303535 4.620094268403453E-4 40-41 0.0 9.240188536806905E-5 0.0 0.33819090044713274 5.082103695243798E-4 42-43 0.0 9.240188536806905E-5 0.0 0.43948646728187846 5.082103695243798E-4 44-45 0.0 9.240188536806905E-5 0.0 0.5608332432414951 5.082103695243798E-4 46-47 0.0 9.240188536806905E-5 0.0 0.706643418352308 5.31310840866397E-4 48-49 0.0 9.240188536806905E-5 0.0 0.8623867961401885 5.31310840866397E-4 50-51 0.0 9.240188536806905E-5 0.0 1.0323138633320674 5.31310840866397E-4 52-53 0.0 9.240188536806905E-5 0.0 1.2103145452579813 6.468131975764834E-4 54-55 0.0 1.0395212103907767E-4 0.0 1.4127324253924076 6.930141402605179E-4 56-57 0.0 1.1550235671008631E-4 0.0 1.6305352194406173 6.930141402605179E-4 58-59 0.0 1.1550235671008631E-4 0.0 1.8850562126869637 6.930141402605179E-4 60-61 0.0 1.1550235671008631E-4 0.0 2.1393115505128764 6.930141402605179E-4 62-63 0.0 1.1550235671008631E-4 0.0 2.403211335124082 6.930141402605179E-4 64-65 0.0 1.1550235671008631E-4 0.0 2.6664527563020393 7.045643759315265E-4 66-67 0.0 1.1550235671008631E-4 0.0 2.9620463875944925 7.161146116025351E-4 68-69 0.0 1.1550235671008631E-4 0.0 3.29617160508543 7.161146116025351E-4 70-71 0.0 1.1550235671008631E-4 0.0 3.6391212026290187 7.738657899575783E-4 72-73 0.0 1.1550235671008631E-4 0.0 3.969261588813458 9.702197963647251E-4 74-75 0.0 1.1550235671008631E-4 0.0 4.302208682265952 9.702197963647251E-4 76-77 0.0 1.3860282805210357E-4 0.0 4.673225352490093 9.702197963647251E-4 78-79 0.0 1.3860282805210357E-4 0.0 5.072748004350281 9.702197963647251E-4 80-81 0.0 1.3860282805210357E-4 0.0 5.480609926364938 0.0010279709747197682 82-83 0.0 1.3860282805210357E-4 0.0 5.903960714414417 0.001131923095758846 84-85 0.0 1.6170329939412084E-4 0.0 6.3421189045941295 0.001131923095758846 86-87 0.0 1.848037707361381E-4 0.0 6.777435736798774 0.001131923095758846 88 0.0 1.848037707361381E-4 0.0 7.11589229266634 0.001131923095758846 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7645 0.0 52.181343 1 GTATCAA 14140 0.0 40.29426 1 ATCAACG 17210 0.0 32.391354 3 CAACGCA 17315 0.0 32.106583 5 TATCAAC 18135 0.0 31.177258 2 TCAACGC 18100 0.0 30.850838 4 AACGCAG 18530 0.0 30.2535 6 TACCTGG 3275 0.0 28.510855 2 TGGTATC 3785 0.0 27.520452 2 GTGGTAT 3890 0.0 27.025406 1 ACGCAGA 20905 0.0 26.547138 7 CGCAGAG 21310 0.0 26.042606 8 GTACATG 19255 0.0 25.958523 1 CGTTAAC 515 0.0 25.516708 1 TACATGG 19675 0.0 24.945108 2 ACATGGG 19675 0.0 24.278765 3 GTACCTG 4075 0.0 23.840569 1 GCAGAGT 23225 0.0 23.651802 9 ACCTGGG 4110 0.0 22.491476 3 CATGGGG 13210 0.0 22.165644 4 >>END_MODULE