FastQCFastQC Report
Mon 6 Jun 2016
ERR1211291_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211291_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7414218
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT229910.3100933908336658No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176940.23864957841811504No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109710.147972449690581No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109850.050.6102371
GTATCAA202650.042.0564421
CAACGCA247850.033.6150055
ATCAACG249750.033.2280163
TATCAAC258400.032.5088122
TCAACGC262550.031.7508224
AACGCAG267950.031.2863186
ACGCAGA299400.027.6237747
GTACATG279850.026.9755731
CGCAGAG308250.026.813638
TACATGG286900.025.9842912
GTACTAG26650.024.8852981
ACATGGG291250.024.6372573
GTGGTAT52650.024.5671771
GCAGAGT341600.023.606759
TGGTATC55900.022.296992
CATGGGG205100.021.9947454
GAGTACT195900.020.5331512-13
TACCTGG53150.020.5303752
ATGGGGG137600.020.1486955
Produced by FastQC (version 0.11.5)