##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211291_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7414218 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.394152019808427 31.0 31.0 34.0 28.0 34.0 2 31.4075214675371 31.0 31.0 34.0 28.0 34.0 3 31.459570921707453 31.0 31.0 34.0 28.0 34.0 4 35.06982260300412 37.0 35.0 37.0 32.0 37.0 5 35.114273278719345 37.0 35.0 37.0 32.0 37.0 6 35.178120066067656 37.0 35.0 37.0 32.0 37.0 7 35.11190175417016 37.0 35.0 37.0 32.0 37.0 8 35.111786300321896 37.0 35.0 37.0 32.0 37.0 9 36.67492863036938 39.0 37.0 39.0 32.0 39.0 10-11 36.60462290156561 39.0 37.0 39.0 32.0 39.0 12-13 36.51852804975522 39.0 37.0 39.0 32.0 39.0 14-15 37.65591448754272 40.0 37.0 41.0 32.0 41.0 16-17 37.65340943576248 40.0 37.0 41.0 32.0 41.0 18-19 37.64659604020275 40.0 37.0 41.0 32.0 41.0 20-21 37.61126567899676 40.0 37.0 41.0 32.0 41.0 22-23 37.48945863204993 40.0 37.0 41.0 31.5 41.0 24-25 37.37924168132095 40.0 37.0 41.0 31.0 41.0 26-27 37.23574664246452 40.0 36.5 41.0 31.0 41.0 28-29 37.09643667073183 40.0 36.0 41.0 30.5 41.0 30-31 36.91489763316913 39.0 36.0 41.0 30.0 41.0 32-33 36.793707171815015 39.0 36.0 41.0 30.0 41.0 34-35 36.65042766479216 39.0 35.0 41.0 30.0 41.0 36-37 36.55536767330014 39.0 35.0 41.0 30.0 41.0 38-39 36.40754655447142 39.0 35.0 41.0 29.0 41.0 40-41 36.236945150520256 39.0 35.0 40.0 28.5 41.0 42-43 36.08651478820828 39.0 35.0 40.0 28.0 41.0 44-45 35.94966266165899 39.0 35.0 40.0 27.5 41.0 46-47 35.78633053411701 38.5 35.0 40.0 27.0 41.0 48-49 35.55327574668023 38.0 34.0 40.0 26.0 41.0 50-51 34.89129750433559 37.5 33.5 39.5 25.0 40.5 52-53 35.13357308889488 38.0 34.0 40.0 26.0 40.5 54-55 35.48992705636657 38.0 34.0 40.0 26.0 41.0 56-57 35.389940382114474 38.0 34.0 40.0 26.0 41.0 58-59 35.16449172387432 38.0 34.0 40.0 26.0 41.0 60-61 34.89703978221304 37.5 34.0 40.0 26.0 41.0 62-63 34.62035369070615 37.0 33.0 40.0 25.5 41.0 64-65 34.33750673368385 36.5 33.0 40.0 25.0 41.0 66-67 34.1128046140537 36.0 33.0 39.0 25.0 41.0 68-69 33.77295960275244 36.0 33.0 39.0 24.0 41.0 70-71 33.42809862078509 35.0 32.0 39.0 24.0 40.5 72-73 33.06556672598512 35.0 32.0 38.0 23.0 40.0 74-75 32.69395767699304 35.0 31.5 37.0 22.5 39.5 76-77 32.33993065755553 35.0 31.0 36.5 22.0 39.0 78-79 31.977593523686515 35.0 31.0 36.0 21.0 39.0 80-81 31.640768857888993 35.0 31.0 36.0 20.0 37.5 82-83 31.32534988855197 34.0 30.5 35.0 20.0 37.0 84-85 31.02585552515451 34.0 30.0 35.0 20.0 37.0 86-87 30.772206994722843 34.0 30.0 35.0 19.0 36.0 88-89 30.5092925511497 34.0 29.5 35.0 18.0 36.0 90-91 30.249840576578677 34.0 29.0 35.0 18.0 35.5 92-93 30.01653269434484 34.0 29.0 35.0 17.5 35.0 94-95 29.795597391390437 34.0 29.0 35.0 17.0 35.0 96-97 29.591233357314287 34.0 29.0 35.0 15.0 35.0 98-99 29.34946443441507 34.0 27.5 35.0 14.0 35.0 100 27.65979959046254 32.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.43027533481364344 1101 2 -0.4362952815150436 1101 3 -0.47427068532970296 1101 4 -0.47480957624487274 1101 5 -0.4099459017027769 1101 6 -0.4269949540023745 1101 7 -0.5569522508087914 1101 8 -0.5247784891788569 1101 9 -0.6436932839855984 1101 10-11 -0.6300780476038454 1101 12-13 -0.6386642273091212 1101 14-15 -0.7498052780766216 1101 16-17 -0.7303959884143225 1101 18-19 -0.7484740595737094 1101 20-21 -0.8688779636958586 1101 22-23 -0.8954883004494008 1101 24-25 -0.827858887024675 1101 26-27 -0.8680113211559544 1101 28-29 -0.8873967627516208 1101 30-31 -0.8855531875745086 1101 32-33 -1.0113867842180042 1101 34-35 -1.0177461349248702 1101 36-37 -1.0558421361649692 1101 38-39 -1.060156143829694 1101 40-41 -1.0361228095593376 1101 42-43 -1.0691160608748262 1101 44-45 -1.2406568710750676 1101 46-47 -1.0577637746661992 1101 48-49 -1.0667699407230558 1101 50-51 -0.9570292576148631 1101 52-53 -0.7831641799162412 1101 54-55 -0.7428088699616211 1101 56-57 -0.7219467008228193 1101 58-59 -0.7838615422939483 1101 60-61 -0.8602647005688056 1101 62-63 -0.846289566583657 1101 64-65 -0.8511134392201996 1101 66-67 -0.8992017517877997 1101 68-69 -0.8828412882506598 1101 70-71 -0.8951137891120027 1101 72-73 -1.0054027641651118 1101 74-75 -0.993710660375001 1101 76-77 -0.9681260606977844 1101 78-79 -1.0045891506521514 1101 80-81 -1.0018535226376812 1101 82-83 -1.1050853860392422 1101 84-85 -1.1121335112448882 1101 86-87 -1.1011740111183563 1101 88-89 -1.14016504316697 1101 90-91 -1.15394369188326 1101 92-93 -1.1015043085418412 1101 94-95 -1.158526250220337 1101 96-97 -1.289444545110488 1101 98-99 -1.3120508355435625 1101 100 -1.197572742899876 1102 1 -0.3860700626559179 1102 2 -0.4000006078968745 1102 3 -0.4077116395510707 1102 4 -0.30283435601315034 1102 5 -0.330202957089476 1102 6 -0.22618623950926064 1102 7 -0.3269687350329846 1102 8 -0.27373640940043487 1102 9 -0.3544318988796604 1102 10-11 -0.39280076549749765 1102 12-13 -0.3624548893276014 1102 14-15 -0.4711241825970234 1102 16-17 -0.5257032533067374 1102 18-19 -0.49419258754501527 1102 20-21 -0.6117135407005918 1102 22-23 -0.5610142365905375 1102 24-25 -0.5845259316678479 1102 26-27 -0.5486145951781864 1102 28-29 -0.7089629090316976 1102 30-31 -0.6569177493201224 1102 32-33 -0.6877592697881312 1102 34-35 -0.7131166084662155 1102 36-37 -0.7237546410695046 1102 38-39 -0.8097199026168624 1102 40-41 -0.8001429333996057 1102 42-43 -0.7494244388136266 1102 44-45 -0.8038266461984165 1102 46-47 -0.9624716776412043 1102 48-49 -1.0241236776184763 1102 50-51 -0.992987652963464 1102 52-53 -0.7255292841909551 1102 54-55 -0.6157605057557518 1102 56-57 -0.6795199505327716 1102 58-59 -0.6458462506640359 1102 60-61 -0.7667420400533302 1102 62-63 -0.7980669338787365 1102 64-65 -0.8632252657929342 1102 66-67 -0.6587738306987276 1102 68-69 -0.7455895747665124 1102 70-71 -0.8360386420864216 1102 72-73 -0.8189867572090677 1102 74-75 -0.7841488934055825 1102 76-77 -0.8319379222053342 1102 78-79 -0.8600097057600955 1102 80-81 -0.9881802386457998 1102 82-83 -0.9199414252388323 1102 84-85 -0.9240491228858438 1102 86-87 -1.1008971297279118 1102 88-89 -1.1511848384941352 1102 90-91 -1.1565496054360658 1102 92-93 -1.1893649906594916 1102 94-95 -1.1085327887977776 1102 96-97 -1.1739717793176752 1102 98-99 -1.2586612144358824 1102 100 -1.2509717974177406 1103 1 -0.45469320807223923 1103 2 -0.42944115956701623 1103 3 -0.42002607707021156 1103 4 -0.3017065283515734 1103 5 -0.35550851925988525 1103 6 -0.4193456088646812 1103 7 -0.44300257137719967 1103 8 -0.42465647485154534 1103 9 -0.6113382816119 1103 10-11 -0.5916730514137356 1103 12-13 -0.6716276746833358 1103 14-15 -0.7450687513250429 1103 16-17 -0.7892538196624983 1103 18-19 -0.7417579998457171 1103 20-21 -0.7987862988355019 1103 22-23 -0.8662192047868587 1103 24-25 -0.9176568441027584 1103 26-27 -0.8041217702670806 1103 28-29 -0.8363912499513972 1103 30-31 -0.955402602393967 1103 32-33 -0.8752809205583176 1103 34-35 -1.0162340401614642 1103 36-37 -1.0604032949263598 1103 38-39 -1.0774428208396998 1103 40-41 -0.9871221791369393 1103 42-43 -1.0064516162800956 1103 44-45 -1.0240796947215074 1103 46-47 -1.0558528928556257 1103 48-49 -1.0743279567760808 1103 50-51 -0.9528028296848561 1103 52-53 -0.8774277041721632 1103 54-55 -0.8350970050074622 1103 56-57 -0.7800508652770972 1103 58-59 -0.7661413608364 1103 60-61 -0.8074187262389714 1103 62-63 -0.9286754061776819 1103 64-65 -0.7697467049298012 1103 66-67 -0.8856184857837377 1103 68-69 -0.8200275018427376 1103 70-71 -0.8420289961122478 1103 72-73 -1.0096898644329535 1103 74-75 -0.9013795322694094 1103 76-77 -0.8701961818676835 1103 78-79 -0.8987132377649978 1103 80-81 -0.9030810700732381 1103 82-83 -1.005954824202469 1103 84-85 -0.9226026858028398 1103 86-87 -1.1264853350129904 1103 88-89 -1.0860508507506914 1103 90-91 -1.058994878914401 1103 92-93 -1.1038389824765886 1103 94-95 -1.2786338276182967 1103 96-97 -1.3664983073083583 1103 98-99 -1.2396943352398928 1103 100 -1.3947075457686857 1104 1 -0.34448955384220525 1104 2 -0.4296488615589844 1104 3 -0.4379758861104399 1104 4 -0.22682274853141848 1104 5 -0.18383071135622941 1104 6 -0.23557383546647515 1104 7 -0.31636554658435756 1104 8 -0.2841247641931872 1104 9 -0.4218780868739671 1104 10-11 -0.4513546096852181 1104 12-13 -0.47855314553125794 1104 14-15 -0.566567004778797 1104 16-17 -0.5211761205763707 1104 18-19 -0.6110787570523257 1104 20-21 -0.6589478904791761 1104 22-23 -0.6255515550265685 1104 24-25 -0.6242752155130304 1104 26-27 -0.728231099313966 1104 28-29 -0.6572601633789787 1104 30-31 -0.7234350183511467 1104 32-33 -0.7908988175366574 1104 34-35 -0.7256012481737386 1104 36-37 -0.7960951834101948 1104 38-39 -0.7463510081398326 1104 40-41 -0.7725537948721026 1104 42-43 -0.722595996056036 1104 44-45 -0.6926536242085533 1104 46-47 -0.714389391816681 1104 48-49 -0.7076728886634385 1104 50-51 -0.6159393446198038 1104 52-53 -0.5226424862969452 1104 54-55 -0.5936916899712301 1104 56-57 -0.5662726641359939 1104 58-59 -0.6168410214096127 1104 60-61 -0.6361647302357554 1104 62-63 -0.675478580602082 1104 64-65 -0.6374256295335883 1104 66-67 -0.7039206037781653 1104 68-69 -0.6268799583898002 1104 70-71 -0.6674996393024486 1104 72-73 -0.7835099634526728 1104 74-75 -0.7164428541173677 1104 76-77 -0.723872233379435 1104 78-79 -0.737597958351639 1104 80-81 -0.7776565565545965 1104 82-83 -0.874725800286356 1104 84-85 -0.8283353228916752 1104 86-87 -0.8205369997850589 1104 88-89 -0.8127074610431428 1104 90-91 -0.7711687322678209 1104 92-93 -0.806405117698521 1104 94-95 -0.7584712987967386 1104 96-97 -0.8295721757891279 1104 98-99 -0.8173119186294002 1104 100 -1.0434402070575963 1105 1 -0.3864158255193182 1105 2 -0.3896445884760098 1105 3 -0.4785103396527397 1105 4 -0.43494646317097363 1105 5 -0.3651235664713255 1105 6 -0.420852331405257 1105 7 -0.3276053189973638 1105 8 -0.3382521852967386 1105 9 -0.4948637749566416 1105 10-11 -0.5468152211231327 1105 12-13 -0.5428434363744117 1105 14-15 -0.5777012943433135 1105 16-17 -0.7044066606238957 1105 18-19 -0.700092750043737 1105 20-21 -0.7127985788017028 1105 22-23 -0.7014789314573875 1105 24-25 -0.5866875494897457 1105 26-27 -0.6344544140696584 1105 28-29 -0.8397739088661851 1105 30-31 -0.7849075673591202 1105 32-33 -0.722317935454754 1105 34-35 -0.909548527235053 1105 36-37 -0.8574465410127843 1105 38-39 -0.7888464094986816 1105 40-41 -0.8122257331038369 1105 42-43 -0.8971926399750743 1105 44-45 -0.9177867286768802 1105 46-47 -0.9080029880237177 1105 48-49 -0.9195546255819096 1105 50-51 -0.8844408196133031 1105 52-53 -0.8317395271181027 1105 54-55 -0.7499645022341781 1105 56-57 -0.8398792312637369 1105 58-59 -0.7144689804368127 1105 60-61 -0.7285803923379888 1105 62-63 -0.8274632678919218 1105 64-65 -0.8375571325956344 1105 66-67 -0.854100217906236 1105 68-69 -0.8230778343929401 1105 70-71 -0.7735231554342761 1105 72-73 -0.7964247190689662 1105 74-75 -0.8481349520933463 1105 76-77 -0.7777618820336727 1105 78-79 -0.8150496091576649 1105 80-81 -0.7486213332374625 1105 82-83 -0.8038913846290043 1105 84-85 -0.684696111960708 1105 86-87 -0.801429328914562 1105 88-89 -0.774219019263775 1105 90-91 -0.8734542784157959 1105 92-93 -0.898705709622277 1105 94-95 -0.9026521438802213 1105 96-97 -0.955968168245203 1105 98-99 -1.0592581781675996 1105 100 -0.9247215329362319 1106 1 -0.4765533209611199 1106 2 -0.5459265025044147 1106 3 -0.5143833501256978 1106 4 -0.37506087791965825 1106 5 -0.3670790571991773 1106 6 -0.3877545536794571 1106 7 -0.4160750509700293 1106 8 -0.4646802061724813 1106 9 -0.5852722213634038 1106 10-11 -0.6531365562524627 1106 12-13 -0.5939874881789251 1106 14-15 -0.6762666767056658 1106 16-17 -0.7324703382468911 1106 18-19 -0.7126167742704865 1106 20-21 -0.7413999802282163 1106 22-23 -0.7426005085565777 1106 24-25 -0.8058277272249015 1106 26-27 -0.761964091269526 1106 28-29 -0.758198000389477 1106 30-31 -0.8950660491151723 1106 32-33 -0.9052073490605608 1106 34-35 -0.8820476634366514 1106 36-37 -0.9432252087539297 1106 38-39 -0.8524888745719963 1106 40-41 -1.010224399930685 1106 42-43 -0.9773400438489332 1106 44-45 -0.9319022469612364 1106 46-47 -0.9315988664561843 1106 48-49 -0.9745952533427413 1106 50-51 -0.9727849101159904 1106 52-53 -0.8149765757236338 1106 54-55 -0.979972932906513 1106 56-57 -0.9310552196883037 1106 58-59 -0.816430913771768 1106 60-61 -0.8302910746270911 1106 62-63 -0.8670000805874807 1106 64-65 -0.8561290213532828 1106 66-67 -0.9101794343321217 1106 68-69 -0.9158623908470886 1106 70-71 -0.886682074139415 1106 72-73 -0.8501415080140475 1106 74-75 -0.9485378276649179 1106 76-77 -0.8722649454614597 1106 78-79 -0.856845640043904 1106 80-81 -1.008906338866769 1106 82-83 -0.8769627008876597 1106 84-85 -0.8664444080187046 1106 86-87 -0.8045134559678786 1106 88-89 -0.8282775002756289 1106 90-91 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0.8344636839288881 2215 48-49 0.7947174397916825 2215 50-51 0.5373351120796244 2215 52-53 0.5910560637418598 2215 54-55 0.6922504857166913 2215 56-57 0.6799588120282252 2215 58-59 0.6898977986976291 2215 60-61 0.676585142970417 2215 62-63 0.6959684130828308 2215 64-65 0.7058004665128195 2215 66-67 0.7038615115745301 2215 68-69 0.7112200703528799 2215 70-71 0.7397888078139232 2215 72-73 0.7562240978109287 2215 74-75 0.7496089275157374 2215 76-77 0.7145324438967222 2215 78-79 0.6399298033649394 2215 80-81 0.7617222817115241 2215 82-83 0.8064600699072315 2215 84-85 0.6344295561928917 2215 86-87 0.7533852173692814 2215 88-89 0.8734705167367132 2215 90-91 0.8709221246019219 2215 92-93 0.9019034043056848 2215 94-95 1.0164440683352751 2215 96-97 0.9941779294691422 2215 98-99 1.0142992017136372 2215 100 0.933266337324838 2216 1 0.18885761333250883 2216 2 -0.0014834775680938606 2216 3 0.1529204450179904 2216 4 0.08880517019634482 2216 5 0.07498435755181987 2216 6 0.08760535816909254 2216 7 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0.6428286804192531 2301 98-99 0.596095150702606 2301 100 0.7234231386300074 2302 1 0.5781844441607795 2302 2 0.5887016274940784 2302 3 0.6181055500801591 2302 4 0.46136014677603754 2302 5 0.47722339515598833 2302 6 0.4959778821067147 2302 7 0.501270179525541 2302 8 0.5497176793077685 2302 9 0.6693711506787423 2302 10-11 0.6517958228691754 2302 12-13 0.6515109266485339 2302 14-15 0.8532960380278354 2302 16-17 0.8071399854582566 2302 18-19 0.784269563120219 2302 20-21 0.77949255798449 2302 22-23 0.7867335037948635 2302 24-25 0.7892110772250547 2302 26-27 0.8616616322792581 2302 28-29 0.8107369035318541 2302 30-31 0.8576412068148827 2302 32-33 0.8760128572270531 2302 34-35 0.9253012072175721 2302 36-37 0.8096489565200358 2302 38-39 0.8235356238751095 2302 40-41 0.8185080537873048 2302 42-43 0.9180813179649121 2302 44-45 0.8349068401658784 2302 46-47 0.8272464321802744 2302 48-49 0.827449117142919 2302 50-51 0.7840109791944982 2302 52-53 0.8487764099237296 2302 54-55 0.7793347914025688 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0.8386103722949869 2303 16-17 0.8872319839903824 2303 18-19 0.8580739345883757 2303 20-21 0.8663043499091359 2303 22-23 0.9156543281590643 2303 24-25 0.9177773740688338 2303 26-27 0.849360289810619 2303 28-29 0.9050803129744551 2303 30-31 0.9600451087592603 2303 32-33 0.8748705118682452 2303 34-35 0.8427112789524003 2303 36-37 0.906643006363197 2303 38-39 0.8940930481896103 2303 40-41 0.8940625982587065 2303 42-43 0.8502097042867476 2303 44-45 0.8654446068171708 2303 46-47 0.8611304747509223 2303 48-49 0.9040315271341584 2303 50-51 0.9177538471779911 2303 52-53 0.9204744784238983 2303 54-55 0.8485574859028588 2303 56-57 0.8862024622831157 2303 58-59 0.8131652703412655 2303 60-61 0.8451549816905626 2303 62-63 0.8957600367300032 2303 64-65 0.8676653837076742 2303 66-67 0.836150098020056 2303 68-69 0.823082453277614 2303 70-71 0.7415795578712405 2303 72-73 0.7424377229339143 2303 74-75 0.6863862623135546 2303 76-77 0.7061976080788881 2303 78-79 0.6611126587649387 2303 80-81 0.6752096809316477 2303 82-83 0.6841480010443952 2303 84-85 0.6550086891014075 2303 86-87 0.7274328042086218 2303 88-89 0.6651469776922738 2303 90-91 0.6940391772519092 2303 92-93 0.6701257444527613 2303 94-95 0.6237809611072684 2303 96-97 0.652445840772824 2303 98-99 0.6709784155755436 2303 100 0.8657802452832826 2304 1 0.6048907772032095 2304 2 0.6232774723200727 2304 3 0.6564813323732892 2304 4 0.5272645716989075 2304 5 0.5189951865708196 2304 6 0.5587424453871535 2304 7 0.5529131486611334 2304 8 0.6008341414699601 2304 9 0.7160615036353377 2304 10-11 0.7050168224700286 2304 12-13 0.7087872584407862 2304 14-15 0.8178326348091218 2304 16-17 0.843208317668676 2304 18-19 0.8194069481383934 2304 20-21 0.9128229666136392 2304 22-23 0.9715587694812484 2304 24-25 0.9091773742749538 2304 26-27 0.9105598909942856 2304 28-29 0.9272891102843843 2304 30-31 0.9249509500904836 2304 32-33 0.9242995175408666 2304 34-35 0.8906298678893378 2304 36-37 0.9358627438846909 2304 38-39 0.9682326182989414 2304 40-41 1.0060670095607165 2304 42-43 0.9643133635531953 2304 44-45 0.9705875456936539 2304 46-47 0.9936885470051635 2304 48-49 1.0215448319313936 2304 50-51 1.0832198246320957 2304 52-53 0.9295388339346147 2304 54-55 0.8551554389067846 2304 56-57 0.8914841800554925 2304 58-59 0.906171320459201 2304 60-61 0.9117509853707162 2304 62-63 0.8859070443045383 2304 64-65 0.8861936472091401 2304 66-67 0.8655110584536416 2304 68-69 0.8444120820124184 2304 70-71 0.821566451466289 2304 72-73 0.7567611080907 2304 74-75 0.8025876309920719 2304 76-77 0.8179819254158431 2304 78-79 0.8471315831063002 2304 80-81 0.7792846362584207 2304 82-83 0.8218895224798217 2304 84-85 0.8926379477444293 2304 86-87 0.9218032263711997 2304 88-89 0.9656151613862995 2304 90-91 0.8873209963184472 2304 92-93 0.8422719764202284 2304 94-95 0.80268271150873 2304 96-97 0.9408414310020028 2304 98-99 0.8959847501416327 2304 100 1.0746592825293533 2305 1 0.6472755662396779 2305 2 0.6854006608288579 2305 3 0.6513378923907069 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0.9831118252433413 2305 68-69 0.9765107811304929 2305 70-71 0.9283942535452354 2305 72-73 0.8585147427252195 2305 74-75 0.9041604302431878 2305 76-77 0.8957789139340306 2305 78-79 0.884851425394718 2305 80-81 0.9455206317963736 2305 82-83 0.9038396707334897 2305 84-85 0.9695684594366938 2305 86-87 0.9784925922443399 2305 88-89 0.9987733588543897 2305 90-91 0.9835679607071093 2305 92-93 1.001679238595031 2305 94-95 0.996571374466793 2305 96-97 1.0583138997840464 2305 98-99 0.9841460417389563 2305 100 1.1368142641686383 2306 1 0.5966318462247919 2306 2 0.5872295058613872 2306 3 0.622723331236088 2306 4 0.47553817962562306 2306 5 0.4721709710050348 2306 6 0.4955971485534505 2306 7 0.5263098975247331 2306 8 0.531091591347284 2306 9 0.6453585984680643 2306 10-11 0.6414925800042752 2306 12-13 0.6716825138170677 2306 14-15 0.8382173597081461 2306 16-17 0.7928107926987735 2306 18-19 0.7715912079565115 2306 20-21 0.8149268762600528 2306 22-23 0.8581912054787111 2306 24-25 0.8732135029996044 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0.9869288794384161 2306 94-95 0.9603808233525548 2306 96-97 0.8938214693614128 2306 98-99 0.9629496091091561 2306 100 1.0193413222987537 2307 1 0.6032253734986348 2307 2 0.6197523020378242 2307 3 0.6071881162179267 2307 4 0.46874341374189754 2307 5 0.46042072246304144 2307 6 0.49065476306604694 2307 7 0.5623818241736274 2307 8 0.5303725798475583 2307 9 0.6517439039059738 2307 10-11 0.6531355012876787 2307 12-13 0.7276566637107109 2307 14-15 0.9172750414451514 2307 16-17 0.8556174063064432 2307 18-19 0.892843178689553 2307 20-21 0.7270513648691406 2307 22-23 0.8585671261562169 2307 24-25 0.9052664961232963 2307 26-27 1.0008766589503821 2307 28-29 0.9536666448148523 2307 30-31 0.9152084312764899 2307 32-33 0.9623501850226717 2307 34-35 1.04926029620092 2307 36-37 0.9550926548509366 2307 38-39 1.0406253018745062 2307 40-41 1.0134458053895727 2307 42-43 0.9826641728113685 2307 44-45 1.0522824427599673 2307 46-47 1.092061972348148 2307 48-49 0.9160869988377414 2307 50-51 1.0127007057151545 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2310 2 0.46849047876205674 2310 3 0.5025100099678248 2310 4 0.42822341834746425 2310 5 0.42901233526634996 2310 6 0.47652855681526063 2310 7 0.4609011874917357 2310 8 0.4873912921326564 2310 9 0.5883288814468344 2310 10-11 0.6155331998471496 2310 12-13 0.6840717836813965 2310 14-15 0.8485619728964906 2310 16-17 0.8447836139786276 2310 18-19 0.7917813100576154 2310 20-21 0.8139054085417214 2310 22-23 0.8599993498211376 2310 24-25 0.8764397377780497 2310 26-27 0.9813193669958977 2310 28-29 0.9582215535422094 2310 30-31 0.8739446479765363 2310 32-33 0.9308240424831666 2310 34-35 0.9608380373213166 2310 36-37 0.7495232692254845 2310 38-39 0.977470230944995 2310 40-41 1.0041009039496842 2310 42-43 1.0269157058902394 2310 44-45 1.1354326143285363 2310 46-47 1.0942249520661704 2310 48-49 1.0698767846354968 2310 50-51 1.0767280013952245 2310 52-53 0.7801121058063814 2310 54-55 0.7655763143584551 2310 56-57 0.8934329498226816 2310 58-59 0.8705256735500981 2310 60-61 0.9093128438688609 2310 62-63 0.906359174931815 2310 64-65 0.8934897297727815 2310 66-67 0.8732505066306544 2310 68-69 0.8542802789425323 2310 70-71 0.9297852877893291 2310 72-73 0.9175531493061015 2310 74-75 0.9476482170927554 2310 76-77 0.9149492854834236 2310 78-79 0.9383253332531645 2310 80-81 0.9313506762549686 2310 82-83 0.918928702471888 2310 84-85 0.9039138377085365 2310 86-87 0.9786991492537105 2310 88-89 0.8188329022584888 2310 90-91 0.7418535737039242 2310 92-93 0.9181859532051142 2310 94-95 1.0548466174094315 2310 96-97 1.0923876067528404 2310 98-99 1.1301584544526442 2310 100 1.223981080959927 2311 1 0.5581815359748639 2311 2 0.5868365673205886 2311 3 0.5604020222785451 2311 4 0.5563386110321886 2311 5 0.4677846983733076 2311 6 0.3652307000134343 2311 7 0.4100475921676576 2311 8 0.4589163267825427 2311 9 0.6468013965123376 2311 10-11 0.6267452796480484 2311 12-13 0.6476183985569719 2311 14-15 0.8314679150241062 2311 16-17 0.7411223639517956 2311 18-19 0.7605002406021057 2311 20-21 0.7871145282352288 2311 22-23 0.8329770726786165 2311 24-25 0.8133609943548876 2311 26-27 0.9006015051708189 2311 28-29 0.9061409415640398 2311 30-31 0.8827673424512668 2311 32-33 0.9419127219978662 2311 34-35 0.9205623445195315 2311 36-37 0.9223872702505815 2311 38-39 0.9701269426630432 2311 40-41 0.9828867214706136 2311 42-43 1.0148049271257236 2311 44-45 1.0665834676853123 2311 46-47 1.0455872114807647 2311 48-49 1.0377587674780528 2311 50-51 0.9697971038515405 2311 52-53 0.9066240489843125 2311 54-55 0.9801814760414587 2311 56-57 0.8924834730555702 2311 58-59 0.8394562009951585 2311 60-61 0.9051039773827867 2311 62-63 0.8977888699957717 2311 64-65 0.9203304336690081 2311 66-67 0.9040637387128214 2311 68-69 0.925618049142642 2311 70-71 0.8543952103173922 2311 72-73 0.8071706902086504 2311 74-75 0.8242808512713893 2311 76-77 0.8833326735761347 2311 78-79 0.8184456868711543 2311 80-81 0.8966041409880781 2311 82-83 0.8730893300446496 2311 84-85 0.9391142720121124 2311 86-87 0.9351108352369586 2311 88-89 0.9020518605774122 2311 90-91 1.0158664821713081 2311 92-93 1.0303003443502838 2311 94-95 1.0772802509906683 2311 96-97 1.032904102211976 2311 98-99 1.0356085826410357 2311 100 1.20797864362536 2312 1 0.48054980441573036 2312 2 0.4661356424109968 2312 3 0.5071434387585185 2312 4 0.4263836380184074 2312 5 0.3903789145315102 2312 6 0.42538516781026203 2312 7 0.49336697552833897 2312 8 0.4741009297440115 2312 9 0.6182989102437944 2312 10-11 0.5900015798217311 2312 12-13 0.5780007325210335 2312 14-15 0.8169369842549088 2312 16-17 0.7303673362858589 2312 18-19 0.7096718742386656 2312 20-21 0.7708473968788851 2312 22-23 0.7548713186938514 2312 24-25 0.7713658174353242 2312 26-27 0.8336560128113177 2312 28-29 0.8420905115040895 2312 30-31 0.8467498757091434 2312 32-33 0.8989920456142926 2312 34-35 0.8849752635271315 2312 36-37 0.931016165700143 2312 38-39 0.947290845433507 2312 40-41 0.9376847152809589 2312 42-43 1.0014635999081989 2312 44-45 0.9844243452639958 2312 46-47 1.0027998183235667 2312 48-49 0.9910577228110924 2312 50-51 1.0347190808302784 2312 52-53 0.9589053479251106 2312 54-55 0.8911016330648991 2312 56-57 0.848282565739197 2312 58-59 0.9021738193682154 2312 60-61 0.977402466238253 2312 62-63 0.8832856905611663 2312 64-65 0.8856070255142967 2312 66-67 0.9238516954703968 2312 68-69 0.9267787764909983 2312 70-71 0.9304585867933284 2312 72-73 0.9220052120732447 2312 74-75 0.8635801019510652 2312 76-77 0.9226158742995594 2312 78-79 0.8856818411946428 2312 80-81 0.879964280263291 2312 82-83 0.8592268456581991 2312 84-85 0.908050329329388 2312 86-87 0.9619368829990229 2312 88-89 0.980436828929161 2312 90-91 1.0316430953136582 2312 92-93 1.1016842051864622 2312 94-95 1.1224165140903288 2312 96-97 1.0600365532412646 2312 98-99 1.1190250956728143 2312 100 1.1510939497347188 2313 1 0.48757428742114683 2313 2 0.5167220427995574 2313 3 0.5471952941119227 2313 4 0.46115493578655276 2313 5 0.4540706246659383 2313 6 0.39386433879538885 2313 7 0.4955568599862872 2313 8 0.4734111890202186 2313 9 0.5715693996079381 2313 10-11 0.6370864278425046 2313 12-13 0.6518797573898425 2313 14-15 0.823835272999375 2313 16-17 0.8354804437139975 2313 18-19 0.8201554806872622 2313 20-21 0.8026389525289446 2313 22-23 0.7924213195517638 2313 24-25 0.8237289116894502 2313 26-27 0.8031247547545561 2313 28-29 0.880904627503476 2313 30-31 0.9461067729303707 2313 32-33 0.9604357196204489 2313 34-35 1.0051580660583852 2313 36-37 0.9984761568234362 2313 38-39 1.003368726414955 2313 40-41 1.0287861784375067 2313 42-43 0.8074192846427977 2313 44-45 0.939216217812195 2313 46-47 1.038435520056396 2313 48-49 1.0225614353281145 2313 50-51 0.9418210933806037 2313 52-53 0.8477038341881311 2313 54-55 0.8171793935506528 2313 56-57 0.8834925414396011 2313 58-59 0.8155461062228397 2313 60-61 0.8414080246439397 2313 62-63 0.7901690131285548 2313 64-65 0.8316895991647968 2313 66-67 0.7733235425379235 2313 68-69 0.8187638049277055 2313 70-71 0.8277961301280499 2313 72-73 0.8648996262978272 2313 74-75 0.8367364635605625 2313 76-77 0.8263490463389402 2313 78-79 0.824047761767094 2313 80-81 0.7950195852920565 2313 82-83 0.8385733061191445 2313 84-85 0.8603757256422533 2313 86-87 0.8878427358195715 2313 88-89 0.8364932108674417 2313 90-91 0.812845054940265 2313 92-93 0.8789760514976948 2313 94-95 0.9936435479286878 2313 96-97 1.095655006597294 2313 98-99 1.0521609405289887 2313 100 1.074429752767223 2314 1 0.41804161178833255 2314 2 0.2877058611698615 2314 3 0.38973467719157995 2314 4 0.3256443665803985 2314 5 0.2642227882809607 2314 6 0.3464462595829758 2314 7 0.3669352712070122 2314 8 0.41148619983314916 2314 9 0.4902827781152794 2314 10-11 0.5334685944096194 2314 12-13 0.5422160359582833 2314 14-15 0.6584829844349827 2314 16-17 0.6568167016863811 2314 18-19 0.6410331058254286 2314 20-21 0.6369695328569733 2314 22-23 0.6718144638133552 2314 24-25 0.7433392001028025 2314 26-27 0.7516653530011439 2314 28-29 0.6902828670904526 2314 30-31 0.7292037871896966 2314 32-33 0.8327657507204833 2314 34-35 0.8290879845666836 2314 36-37 0.8137988763258335 2314 38-39 0.8470062771327278 2314 40-41 0.8833791867651755 2314 42-43 0.7221605296053468 2314 44-45 0.821440261640582 2314 46-47 0.7833970672316468 2314 48-49 0.7539867506193048 2314 50-51 0.8616815688747295 2314 52-53 0.8747156357245984 2314 54-55 0.9604979251896211 2314 56-57 0.9021417357907922 2314 58-59 0.9253069654900372 2314 60-61 0.6569609324628871 2314 62-63 0.7631978132713542 2314 64-65 0.8437095773329872 2314 66-67 0.8110129335127283 2314 68-69 0.7685896996544272 2314 70-71 0.8118963848325365 2314 72-73 0.8848248145800426 2314 74-75 0.9271466682166434 2314 76-77 0.8716059751933187 2314 78-79 0.8750060959586001 2314 80-81 0.9035140552527174 2314 82-83 0.8808066662273433 2314 84-85 0.9076485958390812 2314 86-87 0.9297092528216133 2314 88-89 0.6987626415239774 2314 90-91 0.7849909298947857 2314 92-93 1.0170824781517283 2314 94-95 1.030650049415037 2314 96-97 0.9975222787878231 2314 98-99 1.0262661245546916 2314 100 0.9941539304699418 2315 1 0.30996385326320564 2315 2 0.3563433159580285 2315 3 0.3640126977081408 2315 4 0.24237637797219946 2315 5 0.2485891102308102 2315 6 0.23808507093416154 2315 7 0.24010861998685584 2315 8 0.20357616243195764 2315 9 0.1956132152281782 2315 10-11 0.3120116623725977 2315 12-13 0.312782273684725 2315 14-15 0.46402190281317246 2315 16-17 0.35253259910691526 2315 18-19 0.4858929503949412 2315 20-21 0.30719306448153105 2315 22-23 0.2269037190020029 2315 24-25 0.44731937264429433 2315 26-27 0.49222026217800163 2315 28-29 0.44858711338237356 2315 30-31 0.5192169267092908 2315 32-33 0.5211455151279338 2315 34-35 0.40087480942690945 2315 36-37 0.6213706551544647 2315 38-39 0.6234025400096712 2315 40-41 0.6710371997249283 2315 42-43 0.6551354306927664 2315 44-45 0.6145802739245525 2315 46-47 0.6787240854728367 2315 48-49 0.5482582929232649 2315 50-51 0.6203606859602715 2315 52-53 0.2844407045098265 2315 54-55 0.5377402772535049 2315 56-57 0.47496740495919454 2315 58-59 0.48538042961934735 2315 60-61 0.5299534239281911 2315 62-63 0.5044740744172245 2315 64-65 0.6093041164594268 2315 66-67 0.4747507756287632 2315 68-69 0.48999030519753006 2315 70-71 0.3962559489347086 2315 72-73 0.4654847267280289 2315 74-75 0.1294423057429981 2315 76-77 0.4254150077391827 2315 78-79 0.46352727581887976 2315 80-81 0.4900646866787035 2315 82-83 0.4460048692390828 2315 84-85 0.49658898196851453 2315 86-87 0.43741449747733796 2315 88-89 0.4228499808794517 2315 90-91 0.5975854074349272 2315 92-93 0.6118515911259621 2315 94-95 0.6110518583876576 2315 96-97 0.6988162235232771 2315 98-99 0.8638792993615674 2315 100 0.8215071545125667 2316 1 0.14654745215173293 2316 2 0.18274947462433033 2316 3 0.1576236562095339 2316 4 0.09114523181941792 2316 5 0.07448455012391975 2316 6 -0.013255030163939807 2316 7 -0.0026217128942178647 2316 8 0.028564720274417255 2316 9 0.0980007237133691 2316 10-11 0.08224630909705155 2316 12-13 0.08639819581119212 2316 14-15 -0.07441674247395014 2316 16-17 0.13454408379756444 2316 18-19 0.14117738443239602 2316 20-21 0.2291664490910179 2316 22-23 0.11957360290053032 2316 24-25 0.07614656274611065 2316 26-27 -0.06330547898024719 2316 28-29 0.014935757529563887 2316 30-31 -0.6330515935154679 2316 32-33 -0.3411084583969739 2316 34-35 -0.3343692334194728 2316 36-37 -0.16330874453723965 2316 38-39 -0.08639910086856872 2316 40-41 0.1488537703689019 2316 42-43 0.2299506888634042 2316 44-45 0.07526953240631684 2316 46-47 -0.062354413138244524 2316 48-49 -0.003893143388914666 2316 50-51 0.18714509364220078 2316 52-53 -0.18012022572901287 2316 54-55 -0.06008334644135971 2316 56-57 -0.03236147505714371 2316 58-59 -0.4971673004087336 2316 60-61 -0.002284004536896589 2316 62-63 0.09556276838707589 2316 64-65 -0.218737169909069 2316 66-67 -0.4964113648324968 2316 68-69 -0.585833902443774 2316 70-71 -0.3439972854032476 2316 72-73 -0.46430745883548497 2316 74-75 -0.30221934140914897 2316 76-77 -0.017519160000283307 2316 78-79 0.03173326176002078 2316 80-81 0.026269104733575688 2316 82-83 0.15237443201795742 2316 84-85 0.2018633170698827 2316 86-87 0.28905170496307875 2316 88-89 0.2726894612297954 2316 90-91 -0.3950751730145825 2316 92-93 -0.4207653141368297 2316 94-95 0.028424796603008673 2316 96-97 0.2102555553398453 2316 98-99 0.36855672319194 2316 100 0.19413421924346608 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 191.0 1 532.0 2 331.0 3 319.0 4 268.0 5 280.0 6 324.0 7 780.0 8 2242.0 9 3837.0 10 6078.0 11 9741.0 12 14062.0 13 17459.0 14 20336.0 15 22982.0 16 27217.0 17 32381.0 18 39213.0 19 46553.0 20 55903.0 21 67863.0 22 76080.0 23 78294.0 24 78783.0 25 82626.0 26 91688.0 27 103998.0 28 122167.0 29 145689.0 30 176622.0 31 218213.0 32 276886.0 33 358669.0 34 492501.0 35 685247.0 36 1012920.0 37 1444499.0 38 1304622.0 39 295609.0 40 213.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.148827453139475 18.98754635663713 13.369598842151545 23.494027348071853 2 15.786243108484127 19.76701167453034 40.74136184130934 23.70538337567619 3 19.28413066601735 25.29136530758019 29.165061928828557 26.259442097573903 4 12.865946426918903 16.24860277392039 37.830811738776525 33.054639060384176 5 13.731508669196165 37.292598557251935 34.44244669826427 14.533446075287632 6 31.561739201776 36.42609801206401 17.89192316836963 14.120239617790356 7 28.086189733838918 31.93792637865778 21.857520895480697 18.1183629920226 8 26.14732603959989 35.271801424430116 19.915818703251823 18.665053832718176 9 26.486644161042623 16.110279626599667 20.333868652551203 37.06920755980651 10-11 25.338175904023625 25.930985137200135 27.795718013805725 20.935120944970514 12-13 25.85042999384947 23.5019903705826 28.038653673359835 22.608925962208087 14-15 23.19030314903051 25.080377154026777 25.741163032796305 25.988156664146405 16-17 22.64108696161081 27.91186500182792 26.490543650138026 22.956504386423248 18-19 22.32132008937158 27.125194216010907 28.59172706412719 21.961758630490323 20-21 23.24578540293582 26.35472817860385 28.43515172819311 21.96433469026722 22-23 23.201561047911635 26.133802063324158 28.16644745551107 22.49818943325314 24-25 22.84759034677026 26.28290784053466 28.40914251826242 22.460359294432656 26-27 22.71747132377898 26.484614808461938 28.11478180835465 22.683132059404432 28-29 22.531302162258118 26.575890154343103 28.355945820174306 22.536861863224473 30-31 22.98677895968646 26.42626546903279 28.25406997424774 22.332885597033012 32-33 22.85599205774931 26.5838438078005 28.012214280196424 22.547949854253762 34-35 22.98119133551112 26.595141773062974 28.157315184553333 22.266351706872573 36-37 22.939974118941283 26.494312029821305 27.986713103518905 22.57900074771851 38-39 22.93163501205496 26.567622663939584 28.078306833327765 22.422435490677685 40-41 22.975361758703016 26.19410600648728 28.2660574873404 22.564474747469294 42-43 22.760598122103236 26.381067617886767 28.00011120119968 22.85822305881032 44-45 22.7374852661062 26.727013438714692 27.908302614947317 22.627198680231793 46-47 22.785789346929448 26.494647228724798 27.64932807704638 23.07023534729937 48-49 22.953678457198734 26.53090708666114 27.62947813185565 22.885936324284472 50-51 22.867272195815527 26.68758210013876 27.48139108504839 22.963754618997328 52-53 22.75077983052077 26.906938256317513 27.364117167743274 22.978164745418443 54-55 22.688215500937527 26.76410557786263 27.482529750197525 23.065149171002318 56-57 22.894681743385707 26.697542807700987 27.267497954999392 23.14027749391392 58-59 22.715205254143328 26.71724135883538 27.531885658578926 23.035667728442363 60-61 22.853759856766974 26.84589596646363 27.38257449837101 22.91776967839838 62-63 22.844894480193055 26.71891577929409 27.342107599946992 23.09408214056586 64-65 22.600980632087804 26.58895238002702 27.483055328785426 23.327011659099753 66-67 22.73156830934845 26.554391000061806 27.322261719467956 23.391778971121788 68-69 22.54456485162054 26.67243912150813 27.440694740491562 23.342301286379772 70-71 22.630166465651183 26.413886492312894 27.438984479769367 23.516962562266553 72-73 22.771613057862204 26.63094898208513 27.256603404613276 23.340834555439393 74-75 22.786064099485433 26.825166592525086 27.13641279657842 23.25235651141107 76-77 22.719426823449144 26.745544611106194 27.073565466255857 23.461463099188812 78-79 22.74074260518119 26.63233342067462 27.123545499333602 23.503378474810585 80-81 22.63671315656716 26.919790143594057 27.097221213775896 23.346275486062883 82-83 22.962893090545275 26.670939080120775 27.282038597058715 23.084129232275235 84-85 23.019141704321278 26.763021171422963 26.91522982649954 23.302607297756218 86-87 22.66345734588938 26.944104404768478 27.124682678992933 23.267755570349212 88-89 22.876503450861563 26.56119465752807 27.1147914542691 23.44751043734127 90-91 23.002769386940187 26.6040184285671 27.088568351057063 23.30464383343565 92-93 23.04578517505699 26.650964470293832 27.020940161134227 23.28231019351495 94-95 22.77693860958022 26.572342526493863 27.12961080105644 23.521108062869473 96-97 22.924265202312824 26.50092309469826 27.043509492933648 23.531302210055266 98-99 22.8333257740687 26.602935316452413 26.887730611566106 23.67600829791278 100 23.165593987269375 26.458853433613932 26.577451828011533 23.79810075110516 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2146.0 1 1512.0 2 1777.0 3 2338.5 4 1607.5 5 1342.0 6 1899.0 7 3309.5 8 5304.5 9 6304.5 10 5563.5 11 4729.0 12 4897.5 13 5462.0 14 5993.0 15 6489.5 16 7060.5 17 7686.5 18 8373.5 19 9005.0 20 9654.0 21 10550.5 22 11996.5 23 14235.5 24 17155.5 25 20702.0 26 25693.5 27 32031.5 28 39518.5 29 49595.5 30 62692.0 31 76623.0 32 93766.5 33 112947.5 34 131520.0 35 149428.0 36 170015.0 37 194458.5 38 208562.5 39 213974.0 40 217629.0 41 220153.0 42 226553.0 43 238273.5 44 251346.5 45 268793.5 46 285138.5 47 295540.0 48 306158.5 49 311705.0 50 307564.0 51 302138.0 52 294627.0 53 283611.0 54 271734.5 55 252030.0 56 228769.0 57 202053.0 58 176643.0 59 154237.0 60 128044.0 61 103614.0 62 83961.5 63 66677.5 64 50062.5 65 36189.0 66 26868.0 67 20483.5 68 15792.5 69 12078.5 70 9409.5 71 7245.0 72 5595.5 73 4438.5 74 3424.5 75 2584.0 76 1860.0 77 1235.5 78 853.5 79 605.0 80 427.5 81 293.5 82 216.0 83 169.0 84 113.0 85 87.0 86 76.5 87 57.0 88 40.0 89 34.0 90 31.5 91 25.5 92 20.5 93 16.5 94 14.5 95 11.5 96 9.5 97 4.5 98 2.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024938570729913796 2 0.10803566876506733 3 0.017169713650178615 4 0.017749680411339404 5 0.019152390717402698 6 0.04607363851454058 7 0.045925274924476185 8 0.038210368241128057 9 0.03292322939519718 10-11 0.03678742653641962 12-13 0.03585678219874301 14-15 0.030832651535198992 16-17 0.037353905698483646 18-19 0.036274897770742644 20-21 0.047415654624668443 22-23 0.047948414788990555 24-25 0.06671640893213553 26-27 0.05844176688627176 28-29 0.05054477761511733 30-31 0.04863628234292544 32-33 0.043436812891123514 34-35 0.0448462669967352 36-37 0.04055046668441635 38-39 0.045459952755637884 40-41 0.054355024359952736 42-43 0.05699859378291817 44-45 0.05977703919685124 46-47 0.061388807288914354 48-49 0.06071442733407623 50-51 0.05475965233285561 52-53 0.0641402775046539 54-55 0.05075383540111715 56-57 0.0549417349206619 58-59 0.05381552039608223 60-61 0.0490476541153767 62-63 0.0558386602605966 64-65 0.05391667738930795 66-67 0.05200818211711606 68-69 0.05819224630298166 70-71 0.058549667679045854 72-73 0.06118649330246292 74-75 0.051880049925696815 76-77 0.05214305810808368 78-79 0.0618743608563978 80-81 0.06177320386317208 82-83 0.052419553889567316 84-85 0.05832037849440089 86-87 0.055771222265112795 88-89 0.06425492209697638 90-91 0.06983204432348765 92-93 0.0740199438430324 94-95 0.07765485179960989 96-97 0.07342648948277486 98-99 0.0821394784992834 100 0.1267294811131801 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7414218.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.849452995990205 #Duplication Level Percentage of deduplicated Percentage of total 1 74.67795693111708 44.694348731854724 2 14.768084161944724 17.677235177822766 3 4.970210481743249 8.92393135821811 4 2.134426923603795 5.109771353504052 5 1.08832074907316 3.2567700758107465 6 0.5953289939195028 2.137806878324125 7 0.39302160420089693 1.6465489618921174 8 0.25581018168561825 1.2248079555751297 9 0.19727305993721428 1.0626016255278716 >10 0.8566871341041863 8.726519100833967 >50 0.041415694066586246 1.692437904341472 >100 0.020091259267469012 2.1268155615274864 >500 9.550132551875651E-4 0.38464775268936735 >1k 3.282809211138806E-4 0.4131140824507485 >5k 0.0 0.0 >10k+ 8.953116028034884E-5 0.9226434796273916 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22991 0.3100933908336658 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17694 0.23864957841811504 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10971 0.147972449690581 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 5.3950396387049855E-5 0.0 0.0 0.0 6 0.0 5.3950396387049855E-5 0.0 0.0 0.0 7 0.0 6.743799548381232E-5 0.0 0.0 0.0 8 0.0 6.743799548381232E-5 0.0 0.0 1.3487599096762464E-5 9 0.0 6.743799548381232E-5 0.0 0.0 2.6975198193524928E-5 10-11 0.0 6.743799548381232E-5 0.0 6.743799548381232E-6 3.371899774190616E-5 12-13 0.0 6.743799548381232E-5 0.0 2.0231398645143697E-5 4.046279729028739E-5 14-15 0.0 6.743799548381232E-5 0.0 2.6975198193524928E-5 8.092559458057479E-5 16-17 0.0 6.743799548381232E-5 0.0 4.046279729028739E-5 9.441319367733725E-5 18-19 0.0 6.743799548381232E-5 0.0 5.3950396387049855E-5 1.3487599096762465E-4 20-21 0.0 6.743799548381232E-5 0.0 8.766939412895602E-5 1.3487599096762465E-4 22-23 0.0 6.743799548381232E-5 0.0 4.113717724512552E-4 1.483635900643871E-4 24-25 0.0 6.743799548381232E-5 0.0 0.0010452889299990909 1.6185118916114957E-4 26-27 0.0 6.743799548381232E-5 0.0 0.0020231398645143696 1.6185118916114957E-4 28-29 0.0 6.743799548381232E-5 0.0 0.005624328823349947 1.685949887095308E-4 30-31 0.0 8.092559458057479E-5 0.0 0.016872986470049842 1.888263873546745E-4 32-33 0.0 8.092559458057479E-5 0.0 0.04417188704189706 2.4277678374172435E-4 34-35 0.0 1.0115699322571847E-4 0.0 0.08526860148973228 2.4277678374172435E-4 36-37 0.0 1.2138839187086217E-4 0.0 0.14903122621967685 2.4277678374172435E-4 38-39 0.0 1.2138839187086217E-4 0.0 0.24054458609121016 2.562643828384868E-4 40-41 0.0 1.2138839187086217E-4 0.0 0.3383768861395767 2.967271801287742E-4 42-43 0.0 1.2138839187086217E-4 0.0 0.4395271355657468 2.967271801287742E-4 44-45 0.0 1.281321914192434E-4 0.0 0.5598634947070615 2.967271801287742E-4 46-47 0.0 1.3487599096762465E-4 0.0 0.7052935319679028 3.2370237832229915E-4 48-49 0.0 1.3487599096762465E-4 0.0 0.8605695165693806 3.5067757651582404E-4 50-51 0.0 1.3487599096762465E-4 0.0 1.0165468563238902 3.5067757651582404E-4 52-53 0.0 1.3487599096762465E-4 0.0 1.1708854527881432 3.7090897516096774E-4 54-55 0.0 1.483635900643871E-4 0.0 1.3418475151391558 3.9114037380611144E-4 56-57 0.0 1.483635900643871E-4 0.0 1.5423137005143361 3.9114037380611144E-4 58-59 0.0 1.483635900643871E-4 0.0 1.7709959431999436 3.9114037380611144E-4 60-61 0.0 1.483635900643871E-4 0.0 2.0027466146800648 3.9114037380611144E-4 62-63 0.0 1.6185118916114955E-4 0.0 2.238712160877924 4.181155719996364E-4 64-65 0.0 1.7533878825791202E-4 0.0 2.4825207459505507 4.181155719996364E-4 66-67 0.0 1.7533878825791202E-4 0.0 2.7581398334928915 4.181155719996364E-4 68-69 0.0 1.7533878825791202E-4 0.0 3.0597292930960487 4.3160317109639884E-4 70-71 0.0 1.8208258780629325E-4 0.0 3.3772880700297723 4.5857836928992374E-4 72-73 0.0 1.888263873546745E-4 0.0 3.6945164007856257 5.260163647737361E-4 74-75 0.0 1.888263873546745E-4 0.0 4.00532463437142 5.260163647737361E-4 76-77 0.0 1.888263873546745E-4 0.0 4.346736230307768 5.260163647737361E-4 78-79 0.0 1.9557018690305572E-4 0.0 4.723633699467698 5.260163647737361E-4 80-81 0.0 2.1580158554819942E-4 0.0 5.119676815545483 5.52991562967261E-4 82-83 1.3487599096762464E-5 2.1580158554819942E-4 0.0 5.522787433549971 6.204295584510733E-4 84-85 1.3487599096762464E-5 2.2254538509658065E-4 0.0 5.914170584139824 6.271733579994545E-4 86-87 1.3487599096762464E-5 2.2928918464496187E-4 0.0 6.323896869501274 6.339171575478358E-4 88 1.3487599096762464E-5 2.2928918464496187E-4 0.0 6.650249561046087 6.608923557413607E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10985 0.0 50.610237 1 GTATCAA 20265 0.0 42.056442 1 CAACGCA 24785 0.0 33.615005 5 ATCAACG 24975 0.0 33.228016 3 TATCAAC 25840 0.0 32.508812 2 TCAACGC 26255 0.0 31.750822 4 AACGCAG 26795 0.0 31.286318 6 ACGCAGA 29940 0.0 27.623774 7 GTACATG 27985 0.0 26.975573 1 CGCAGAG 30825 0.0 26.81363 8 TACATGG 28690 0.0 25.984291 2 GTACTAG 2665 0.0 24.885298 1 ACATGGG 29125 0.0 24.637257 3 GTGGTAT 5265 0.0 24.567177 1 GCAGAGT 34160 0.0 23.60675 9 TGGTATC 5590 0.0 22.29699 2 CATGGGG 20510 0.0 21.994745 4 GAGTACT 19590 0.0 20.53315 12-13 TACCTGG 5315 0.0 20.530375 2 ATGGGGG 13760 0.0 20.148695 5 >>END_MODULE