FastQCFastQC Report
Mon 6 Jun 2016
ERR1211291_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211291_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7414218
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200760.2707770394666032No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167000.22524290491593313No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107060.14439823592993895No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110100.049.694891
GTATCAA197900.041.3050231
CAACGCA243550.033.0345275
ATCAACG245700.032.7851943
TATCAAC253900.031.9450742
TCAACGC254250.031.7202284
AACGCAG263450.030.717096
ACGCAGA296700.026.974337
CGCAGAG305750.026.1298798
GTACATG274750.025.0890731
GTACTAG26200.024.1787031
TACATGG280250.024.037012
ACATGGG277900.023.4153443
GCAGAGT341350.023.087569
TACCTGG51900.022.054342
GTGGTAT55300.021.8924881
CATGGGG194950.021.514724
GAGTACT194700.020.69365912-13
AGAGTAC319300.019.76544810-11
CAGAGTA337250.019.32539610-11
Produced by FastQC (version 0.11.5)