##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211291_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7414218 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.053814306512166 33.0 31.0 34.0 30.0 34.0 2 32.189586143811795 33.0 31.0 34.0 30.0 34.0 3 32.25489094062247 34.0 31.0 34.0 30.0 34.0 4 35.908512131690756 37.0 35.0 37.0 35.0 37.0 5 35.80518822079416 37.0 35.0 37.0 35.0 37.0 6 35.8216372920246 37.0 35.0 37.0 35.0 37.0 7 35.73579492806928 37.0 35.0 37.0 33.0 37.0 8 35.7184505500108 37.0 35.0 37.0 33.0 37.0 9 37.32775540185088 39.0 37.0 39.0 34.0 39.0 10-11 37.27376899357424 39.0 37.0 39.0 34.0 39.0 12-13 37.19950526137753 39.0 37.0 39.0 33.0 39.0 14-15 38.42042491871699 40.0 38.0 41.0 33.5 41.0 16-17 38.39911822123385 40.0 38.0 41.0 33.0 41.0 18-19 38.401797802546405 40.0 38.0 41.0 33.5 41.0 20-21 38.36756762479873 40.0 38.0 41.0 34.0 41.0 22-23 38.25699682690744 40.0 38.0 41.0 33.5 41.0 24-25 38.16153402826839 40.0 38.0 41.0 33.0 41.0 26-27 38.06010546223486 40.0 37.0 41.0 33.0 41.0 28-29 37.955486202321 40.0 37.0 41.0 33.0 41.0 30-31 37.96938476856224 40.0 37.0 41.0 33.0 41.0 32-33 37.9071332944351 40.0 37.0 41.0 33.0 41.0 34-35 37.7760214226234 40.0 37.0 41.0 33.0 41.0 36-37 37.626224572841 40.0 37.0 41.0 32.5 41.0 38-39 37.50900762561878 40.0 36.5 41.0 32.0 41.0 40-41 37.399685577089855 40.0 36.0 41.0 32.0 41.0 42-43 37.291626372464364 39.5 36.0 41.0 31.5 41.0 44-45 37.13586402503945 39.0 36.0 41.0 31.0 41.0 46-47 36.89914297097819 39.0 35.5 41.0 30.5 41.0 48-49 35.352205519179506 39.0 35.0 40.0 24.0 41.0 50-51 36.08733099026762 38.5 35.0 40.0 28.5 41.0 52-53 36.18439807138123 39.0 35.0 40.0 29.0 41.0 54-55 36.01144820667534 38.0 35.0 40.0 29.0 41.0 56-57 35.78791289924305 38.0 34.5 40.0 28.0 41.0 58-59 35.54650800664345 38.0 34.0 40.0 28.0 41.0 60-61 35.285501046772566 37.5 34.0 40.0 28.0 41.0 62-63 34.960964325570146 37.0 34.0 40.0 27.0 41.0 64-65 34.621864234366996 36.0 33.5 39.0 26.0 41.0 66-67 34.281356577322114 36.0 33.0 39.0 26.0 40.0 68-69 33.913886872492824 35.5 33.0 38.5 26.0 40.0 70-71 33.55039466063717 35.0 32.0 38.0 26.0 40.0 72-73 33.171605218513946 35.0 32.0 37.0 25.0 39.5 74-75 32.8072825751819 35.0 32.0 37.0 25.0 39.0 76-77 31.88804847658917 34.0 30.5 36.0 24.5 38.0 78-79 32.33050215140693 34.5 31.0 36.0 25.0 38.0 80-81 32.20166159128313 34.5 31.0 35.5 25.0 37.0 82-83 31.93268622530387 34.0 31.0 35.0 24.5 37.0 84-85 31.65561688366865 34.0 31.0 35.0 24.0 36.0 86-87 31.389489356800674 34.0 31.0 35.0 23.5 36.0 88-89 31.15600222437484 34.0 31.0 35.0 23.0 36.0 90-91 30.93443691566663 34.0 30.0 35.0 21.5 35.0 92-93 30.739067424238133 34.0 30.0 35.0 20.0 35.0 94-95 30.539139933570876 34.0 30.0 35.0 20.0 35.0 96-97 30.343383482924295 34.0 29.0 35.0 20.0 35.0 98-99 30.112795577362306 34.0 29.0 35.0 19.0 35.0 100 27.65275906373403 31.0 25.0 34.0 12.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.33655083752389103 1101 2 -0.3147753326549676 1101 3 -0.3454551397148222 1101 4 -0.2069498525226905 1101 5 -0.2118124822286589 1101 6 -0.2869079177099252 1101 7 -0.34127180088777465 1101 8 -0.2651389040594694 1101 9 -0.4257262061594389 1101 10-11 -0.4122126834664286 1101 12-13 -0.47533261771670254 1101 14-15 -0.5562202694270582 1101 16-17 -0.571890052359258 1101 18-19 -0.6517397313611184 1101 20-21 -0.7006187523927707 1101 22-23 -0.7438646571422964 1101 24-25 -0.6708202795022089 1101 26-27 -0.714391465274332 1101 28-29 -0.7854654320920318 1101 30-31 -0.659033407406028 1101 32-33 -0.6007527866731337 1101 34-35 -0.7250277817957596 1101 36-37 -0.743149255460601 1101 38-39 -0.7078974828734559 1101 40-41 -0.7028913116203768 1101 42-43 -0.7364470500169631 1101 44-45 -0.6746592230816617 1101 46-47 -0.7560861218904193 1101 48-49 0.5624302370346754 1101 50-51 -0.41201593996064645 1101 52-53 -0.795899171183251 1101 54-55 -0.8152175176411802 1101 56-57 -0.811220636179371 1101 58-59 -0.9486028525568884 1101 60-61 -0.9287540373844507 1101 62-63 -0.9301853954002794 1101 64-65 -0.9952467591396825 1101 66-67 -0.9307077536098163 1101 68-69 -0.9003164707315179 1101 70-71 -0.8590855077633677 1101 72-73 -1.0058879943179235 1101 74-75 -1.149526141572636 1101 76-77 -0.9227268406686164 1101 78-79 -0.8728320595963766 1101 80-81 -0.7518619087653171 1101 82-83 -0.681260940576017 1101 84-85 -0.7477341194007607 1101 86-87 -0.7602511890843147 1101 88-89 -0.7988699029849755 1101 90-91 -0.8861309663815113 1101 92-93 -0.9011214253774789 1101 94-95 -0.9562637333563337 1101 96-97 -1.0457428292554454 1101 98-99 -1.269692916693291 1101 100 -1.1579779305505262 1102 1 -0.3091420522393946 1102 2 -0.30741456030783 1102 3 -0.3099067616588833 1102 4 -0.21649646362649122 1102 5 -0.1904884625436054 1102 6 -0.29706736442529547 1102 7 -0.30328403417249916 1102 8 -0.2681722135028295 1102 9 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-0.867257439033434 1103 94-95 -0.9108443856293214 1103 96-97 -0.9994740569254077 1103 98-99 -1.1605629836641569 1103 100 -1.1808358242045287 1104 1 -0.3628114013155077 1104 2 -0.3052265310279516 1104 3 -0.36217044893921013 1104 4 -0.16819467728662119 1104 5 -0.21626901281261013 1104 6 -0.23770135414648763 1104 7 -0.2840930104290891 1104 8 -0.2792628527500369 1104 9 -0.4738019110564764 1104 10-11 -0.40336895331581246 1104 12-13 -0.38584272621869786 1104 14-15 -0.5712702924211115 1104 16-17 -0.6146114938432916 1104 18-19 -0.597503191992125 1104 20-21 -0.6019653236332587 1104 22-23 -0.6185367670522339 1104 24-25 -0.6504579044068421 1104 26-27 -0.692643111570959 1104 28-29 -0.6853423887884773 1104 30-31 -0.620968415329628 1104 32-33 -0.676541553008768 1104 34-35 -0.7004058896181178 1104 36-37 -0.6377763769844833 1104 38-39 -0.5797973672043 1104 40-41 -0.628680415160062 1104 42-43 -0.6011311296201711 1104 44-45 -0.6612774616477068 1104 46-47 -0.6599054255171737 1104 48-49 0.6541667008536294 1104 50-51 -0.23503871724171432 1104 52-53 -0.6804550465109145 1104 54-55 -0.7314542724940551 1104 56-57 -0.7724367344262006 1104 58-59 -0.807605930868057 1104 60-61 -0.8546170224457441 1104 62-63 -0.8936071697688632 1104 64-65 -0.9375072765394918 1104 66-67 -0.8066290469667621 1104 68-69 -0.8623771425824458 1104 70-71 -0.8939409824622899 1104 72-73 -0.8854666096825312 1104 74-75 -0.7812298949662946 1104 76-77 -0.787762394225684 1104 78-79 -0.6104698036542189 1104 80-81 -0.6539327452761299 1104 82-83 -0.7533161114506015 1104 84-85 -0.6859252257877486 1104 86-87 -0.6559024657819776 1104 88-89 -0.6789141866262653 1104 90-91 -0.8379274039806894 1104 92-93 -0.7935328129982402 1104 94-95 -0.8437532721552508 1104 96-97 -0.9453531380659683 1104 98-99 -0.8672466285711806 1104 100 -0.715585133490162 1105 1 -0.312052365845787 1105 2 -0.28157906499481555 1105 3 -0.3202992794135966 1105 4 -0.15058294095494773 1105 5 -0.25005767433179926 1105 6 -0.32469280933587186 1105 7 -0.4551957446800543 1105 8 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0.6308308359225663 2311 20-21 0.7072893769317901 2311 22-23 0.7399935850094579 2311 24-25 0.6387496385267752 2311 26-27 0.6968210789549474 2311 28-29 0.7825097641805954 2311 30-31 0.6380205891703312 2311 32-33 0.6860808202178177 2311 34-35 0.7684647861950324 2311 36-37 0.8098930100305424 2311 38-39 0.8335539469445123 2311 40-41 0.7807277443365876 2311 42-43 0.8248376442189453 2311 44-45 0.8605083312206503 2311 46-47 0.8672444357557083 2311 48-49 -6.041106107742184 2311 50-51 -1.0181769211607659 2311 52-53 0.4137653151658256 2311 54-55 0.8099326151295401 2311 56-57 0.9499535421737875 2311 58-59 1.0097008371696532 2311 60-61 0.8453110208609402 2311 62-63 0.699288261469384 2311 64-65 0.7574511625096534 2311 66-67 0.9192782922728497 2311 68-69 0.9519324035167713 2311 70-71 0.913811064573224 2311 72-73 0.4133598601400408 2311 74-75 0.7100423097528292 2311 76-77 0.8432936712027335 2311 78-79 0.7703402902361134 2311 80-81 0.7484368900726182 2311 82-83 0.8652714135866546 2311 84-85 0.9020043578267831 2311 86-87 0.9575392979039314 2311 88-89 0.9574845423118425 2311 90-91 1.002601299560986 2311 92-93 0.9890623593841887 2311 94-95 0.984011999638966 2311 96-97 1.0838399449066358 2311 98-99 1.0307548774254904 2311 100 1.2726479104767954 2312 1 0.06667539823135371 2312 2 0.24510523766846148 2312 3 0.206327548079841 2312 4 0.01226664747832018 2312 5 0.1819318688595999 2312 6 0.13656671761091843 2312 7 0.28210759968713717 2312 8 0.3434969403478476 2312 9 0.2846957618507773 2312 10-11 0.2502127173319266 2312 12-13 0.2882422496908532 2312 14-15 0.35804264291245147 2312 16-17 0.5115629642048631 2312 18-19 0.37413560696410997 2312 20-21 0.41444158038158463 2312 22-23 0.6633473344815712 2312 24-25 0.7209083974234431 2312 26-27 0.6947327533021621 2312 28-29 0.761461577204912 2312 30-31 0.7359082914675099 2312 32-33 0.7595640976380835 2312 34-35 0.39397447004586894 2312 36-37 0.6918837862441407 2312 38-39 0.805645432518169 2312 40-41 0.6272305583260334 2312 42-43 0.7050576644268034 2312 44-45 0.8316032173959371 2312 46-47 0.9162136753286703 2312 48-49 -4.496543205322968 2312 50-51 -0.6844693106213313 2312 52-53 0.6359499487868234 2312 54-55 0.8343097207860595 2312 56-57 0.9615871403291081 2312 58-59 0.9402171402556618 2312 60-61 0.9957780300044092 2312 62-63 0.7612568750254596 2312 64-65 0.9903830388819088 2312 66-67 0.9588538079685662 2312 68-69 0.8895598043313058 2312 70-71 0.9316077018879056 2312 72-73 1.0074854321169298 2312 74-75 0.716302581467616 2312 76-77 0.8808076317496152 2312 78-79 0.6042155679466532 2312 80-81 0.8044950523704628 2312 82-83 0.6960288363999183 2312 84-85 0.8073849410424145 2312 86-87 0.9332539307793688 2312 88-89 0.9392613808394721 2312 90-91 0.7543886508099575 2312 92-93 0.9538408195747046 2312 94-95 1.0660199163827677 2312 96-97 1.1007503639963723 2312 98-99 1.184034387619235 2312 100 0.6982321031629652 2313 1 0.11958285403344604 2313 2 0.2503293094225967 2313 3 0.327502663262905 2313 4 0.14355113000286224 2313 5 0.1841677027156834 2313 6 0.2570149299738773 2313 7 0.18380094467615748 2313 8 0.26975404230626054 2313 9 0.4577858813641882 2313 10-11 0.24438754138182617 2313 12-13 0.040945288975606786 2313 14-15 0.10794101283880764 2313 16-17 0.10615583199203371 2313 18-19 0.447724768869854 2313 20-21 0.4172673850749291 2313 22-23 0.5569321932470075 2313 24-25 0.31870018318142 2313 26-27 0.4092638437199767 2313 28-29 0.4636546989437207 2313 30-31 0.5846316978021733 2313 32-33 0.48887375693454516 2313 34-35 0.45143523356328075 2313 36-37 -0.32944012169691206 2313 38-39 0.14972230661087593 2313 40-41 0.45095906768146676 2313 42-43 0.5121587871413453 2313 44-45 0.5230663425735926 2313 46-47 0.3425389378746502 2313 48-49 -5.555890247161798 2313 50-51 -1.4020477831700404 2313 52-53 0.0398072050241538 2313 54-55 0.33100903392168135 2313 56-57 0.30022650701924647 2313 58-59 0.41313347525836974 2313 60-61 0.6970488529250929 2313 62-63 0.7552246699668785 2313 64-65 0.8566790637286843 2313 66-67 0.8628687636508658 2313 68-69 0.9094325303289068 2313 70-71 0.8398416730025886 2313 72-73 0.6324377405286228 2313 74-75 0.8191269021758742 2313 76-77 0.8474571629463661 2313 78-79 0.7189537554947236 2313 80-81 -0.16265424447048105 2313 82-83 0.22931984168445751 2313 84-85 0.6193266940305477 2313 86-87 0.7316474967863762 2313 88-89 0.7734346603787863 2313 90-91 0.7976103274760113 2313 92-93 0.843824231147881 2313 94-95 0.7528509327096025 2313 96-97 0.7651947641418317 2313 98-99 0.944602221674554 2313 100 1.0652947055669628 2314 1 0.07011027767359224 2314 2 0.19317049328353164 2314 3 0.23958018679389426 2314 4 0.06538113020882008 2314 5 0.18672858807120463 2314 6 0.2788754535254583 2314 7 0.11621658668935453 2314 8 0.2565982469211505 2314 9 0.4343118083580606 2314 10-11 0.3550618285868765 2314 12-13 0.2560789985301142 2314 14-15 0.30016647092928395 2314 16-17 0.4555379573670564 2314 18-19 0.5150739817574888 2314 20-21 0.5874364923734845 2314 22-23 0.34364189192680783 2314 24-25 0.48542699031314385 2314 26-27 0.5350425323008352 2314 28-29 0.6479090738879378 2314 30-31 0.5544479261162536 2314 32-33 0.5525498291305624 2314 34-35 0.32250330350746736 2314 36-37 0.500782741291296 2314 38-39 0.5536864763497888 2314 40-41 0.3921874504109937 2314 42-43 0.41524294946567863 2314 44-45 0.6444765472296723 2314 46-47 0.7535340703415727 2314 48-49 -6.331154055096768 2314 50-51 -1.380907935992731 2314 52-53 -0.597204198322018 2314 54-55 -0.1870850831599924 2314 56-57 0.059074055059525676 2314 58-59 0.5439636402327253 2314 60-61 0.7910358705484413 2314 62-63 0.655045285239531 2314 64-65 0.7638085373623085 2314 66-67 0.8637710628565429 2314 68-69 0.9030881094554957 2314 70-71 0.6571249442045044 2314 72-73 0.8250218033053827 2314 74-75 0.915868311906813 2314 76-77 0.8082665259105504 2314 78-79 0.5637133789989264 2314 80-81 0.6085646366147941 2314 82-83 0.684664159577622 2314 84-85 0.6686864246342168 2314 86-87 0.7628914913215645 2314 88-89 0.6494178196755023 2314 90-91 0.8289712251891608 2314 92-93 0.9033728893152428 2314 94-95 0.9048918090028621 2314 96-97 0.9101917227918292 2314 98-99 1.0125420400173937 2314 100 -0.4528734131212069 2315 1 0.13504805387565 2315 2 0.23430573179727077 2315 3 0.29335401195206146 2315 4 0.12769808658075732 2315 5 0.19827653133857837 2315 6 0.29448644118656375 2315 7 0.31911553689267436 2315 8 0.2429246243319625 2315 9 0.41340995194734376 2315 10-11 0.22051412667121184 2315 12-13 0.4139996920251079 2315 14-15 0.24460245530300995 2315 16-17 0.40398388210194014 2315 18-19 0.44744352616870486 2315 20-21 0.2987108726405694 2315 22-23 0.39430132137137264 2315 24-25 0.45179651142142774 2315 26-27 0.5268356156603815 2315 28-29 0.5986105817535119 2315 30-31 0.5789633601415147 2315 32-33 0.6330815419120484 2315 34-35 0.6859109764942204 2315 36-37 0.5884729988385331 2315 38-39 0.6170875645962965 2315 40-41 0.6619110943889339 2315 42-43 0.7317398676541131 2315 44-45 0.7228153684013776 2315 46-47 0.5758814403570369 2315 48-49 -4.984541697556693 2315 50-51 -0.9634119482567272 2315 52-53 0.2883157448188527 2315 54-55 0.222698236274816 2315 56-57 0.5983408119417959 2315 58-59 0.7212055195801739 2315 60-61 0.590707033014759 2315 62-63 0.37582940862518655 2315 64-65 0.5343631902070101 2315 66-67 -0.23120189077230435 2315 68-69 0.580952602958142 2315 70-71 0.4815041826316886 2315 72-73 0.558837134298436 2315 74-75 0.7719642999757141 2315 76-77 0.7886814644157987 2315 78-79 0.6537839968426482 2315 80-81 0.5144821609122516 2315 82-83 0.5132637104970001 2315 84-85 0.5407770663467844 2315 86-87 0.5261369093843236 2315 88-89 0.5655323368971956 2315 90-91 0.6164369757180523 2315 92-93 0.7206653626375576 2315 94-95 0.286676972697574 2315 96-97 0.3845046710610056 2315 98-99 0.3781465061890401 2315 100 0.3106738244004923 2316 1 0.2070661504834561 2316 2 0.2561638417585428 2316 3 0.19510521112874102 2316 4 0.011725873881758275 2316 5 0.1778057160184261 2316 6 0.12981181789923824 2316 7 0.2379809307300178 2316 8 0.2864543333368985 2316 9 0.44984954089104434 2316 10-11 0.3680850345678337 2316 12-13 0.26348443736853255 2316 14-15 0.4396773269751435 2316 16-17 0.44994902701879624 2316 18-19 0.11156924998158502 2316 20-21 0.49163207019091715 2316 22-23 0.6349824581175909 2316 24-25 0.6031245757554942 2316 26-27 0.6346190924349528 2316 28-29 0.7239861815561213 2316 30-31 0.5893633760405379 2316 32-33 0.6255217764591308 2316 34-35 0.5866915943900111 2316 36-37 0.45143283322294536 2316 38-39 0.5215596241975078 2316 40-41 0.08403280921479706 2316 42-43 0.026743571356227847 2316 44-45 0.3991525152158317 2316 46-47 0.42775004887282364 2316 48-49 -4.979402763997914 2316 50-51 -0.9736685622094754 2316 52-53 0.34350969822784094 2316 54-55 0.5486611787370421 2316 56-57 0.47356900557866766 2316 58-59 0.28530574763052385 2316 60-61 0.5146319188395978 2316 62-63 0.3810098307186607 2316 64-65 0.3845300791100925 2316 66-67 0.47832862829024236 2316 68-69 0.22459631570685445 2316 70-71 -0.23413516827657332 2316 72-73 -0.09468204160052096 2316 74-75 0.09599398787479885 2316 76-77 0.10308861789627244 2316 78-79 0.2165690530742097 2316 80-81 0.25577299698684186 2316 82-83 0.18132003103554695 2316 84-85 0.2898820362500558 2316 86-87 0.3356920537269268 2316 88-89 0.17481292972308893 2316 90-91 0.1859540556023127 2316 92-93 0.25025825190447293 2316 94-95 0.46354461832580895 2316 96-97 0.5688508862138519 2316 98-99 0.6362365160070098 2316 100 -0.1999285585895194 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 12.0 11 11.0 12 38.0 13 183.0 14 613.0 15 1843.0 16 4450.0 17 8884.0 18 14731.0 19 22510.0 20 31341.0 21 43748.0 22 54306.0 23 58613.0 24 62450.0 25 71027.0 26 83113.0 27 102961.0 28 127201.0 29 155667.0 30 190902.0 31 236288.0 32 296793.0 33 385938.0 34 531225.0 35 745632.0 36 1143560.0 37 1644648.0 38 1248237.0 39 147274.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.20994138223939 19.74592044778745 14.211568993784207 23.83256917618895 2 15.544488645544554 20.279510888316228 40.03876940324738 24.137231062891836 3 18.795813177794276 25.18483750471223 29.88074934433382 26.138599973159664 4 12.931842381350211 16.722432796338982 37.66621074376319 32.67951407854762 5 13.587974180829953 37.45310506986544 34.51978040460572 14.439140344698895 6 31.695295673164136 36.72474112910372 17.504262419079453 14.075700778652688 7 28.031504346717757 32.03303384107421 21.684817610495514 18.25064420171252 8 26.22882008049452 35.52229650140594 19.686236203488004 18.562647214611538 9 26.34621771115674 15.915183623763973 20.167535490108957 37.571063174970334 10-11 25.31702568076948 26.1127814163922 27.676130511035186 20.894062391803132 12-13 25.708451937201954 23.603056103808207 28.066275152792713 22.622216806197127 14-15 23.027738954069633 25.165052930435973 25.655550066847965 26.151658048646436 16-17 22.571389592138377 28.025435268178526 26.46376170246973 22.939413437213364 18-19 22.271000890114404 27.17202514547898 28.54131364269086 22.015660321715764 20-21 23.12472055369314 26.364301547770058 28.46792951108108 22.04304838745572 22-23 23.056400593725954 26.20142173223121 28.185662772559933 22.5565149014829 24-25 22.739204352719742 26.33121645379855 28.429820284413115 22.499758909068593 26-27 22.72746676500037 26.481831947154987 28.1510928440854 22.639608443759244 28-29 22.468187199780314 26.629525094838876 28.368455794147245 22.533831911233566 30-31 22.914834136752212 26.411522078131576 28.3263743561291 22.347269428987108 32-33 22.844639991593716 26.605710757016876 28.020555201958913 22.529094049430494 34-35 22.90581680437733 26.627002973023306 28.238170214335977 22.22901000826338 36-37 22.895988956133806 26.502074873241536 28.08366783600107 22.51826833462359 38-39 22.8517044917229 26.530900636598453 28.18603773345642 22.43135713822223 40-41 22.853839791569662 26.237548375401204 28.37115107692424 22.537460756104892 42-43 22.72989098572394 26.368212016287636 28.10451741946875 22.797379578519678 44-45 22.637906796622527 26.69602624583974 28.027647589010137 22.6384193685276 46-47 22.70547522746536 26.529681495549635 27.73374741337674 23.03109586360826 48-49 23.000330390762166 26.60255220037517 27.63125523915103 22.76586216971163 50-51 22.767889783753173 26.675069115832663 27.571564935697385 22.985476164716783 52-53 22.69041437094988 26.839607554345474 27.45650520801646 23.013472866688186 54-55 22.641564280127493 26.74458859451801 27.525997562700045 23.08784956265445 56-57 22.858461076322204 26.64812347279823 27.3174400599756 23.175975390903965 58-59 22.622534682929423 26.69208203192891 27.61662293300356 23.068760352138103 60-61 22.769101282158065 26.885110908712623 27.400091787451014 22.945696021678295 62-63 22.77234881069483 26.757193039486815 27.316422922362083 23.15403522745628 64-65 22.48580649482494 26.642051168318844 27.430132934078504 23.44200940277772 66-67 22.578208003777593 26.6028905340911 27.327102551562472 23.491798910568832 68-69 22.422667564207288 26.668262254461673 27.434886425336714 23.474183755994332 70-71 22.45558212559572 26.396652849496277 27.423753166200658 23.724011858707346 72-73 22.56070995161389 26.62483137576666 27.239403005290413 23.575055667329035 74-75 22.59052156418673 26.78641112609116 27.085012406311943 23.53805490341017 76-77 22.5213372738713 26.703990624293066 27.05212633516884 23.722545766666787 78-79 22.438106599433596 26.62947697502709 27.13319220107711 23.7992242244622 80-81 22.388695748935294 26.900233004339746 27.004083476923803 23.706987769801156 82-83 22.618618204617817 26.6631018524264 27.225713443972072 23.492566498983706 84-85 22.684031011098472 26.729301791600214 26.855986317720422 23.7306808795809 86-87 22.361539570886226 26.87905065650298 27.049174688328538 23.710235084282257 88-89 22.404044646483683 26.55321938595401 27.066308421563974 23.976427545998334 90-91 22.50372754158304 26.518417141328882 27.09821047650814 23.879644840579935 92-93 22.54316226925092 26.58390701706783 26.965742331543236 23.907188382138013 94-95 22.180605154591966 26.505759528045054 27.12664568180903 24.18698963555395 96-97 22.271519600451146 26.448422584307824 27.089800298409944 24.190257516831082 98-99 22.140646178849856 26.48562212399313 27.012523580018822 24.361208117138194 100 22.122931454200867 26.415444368424414 26.929962523218077 24.531661654156643 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1999.0 1 1402.5 2 1518.5 3 2030.0 4 1398.5 5 1089.0 6 1592.5 7 3067.0 8 4821.0 9 5585.0 10 5020.5 11 4264.0 12 4320.5 13 4829.5 14 5491.0 15 6106.5 16 6833.0 17 7579.0 18 8291.0 19 8981.5 20 9693.5 21 10660.5 22 12228.5 23 14519.0 24 17214.5 25 20695.0 26 25701.0 27 31935.5 28 39618.5 29 49888.5 30 62850.5 31 76481.0 32 93516.0 33 112784.0 34 131130.5 35 149405.5 36 170243.0 37 194236.0 38 208206.0 39 213567.5 40 216935.0 41 218811.0 42 226056.5 43 239061.5 44 252735.0 45 270498.0 46 287836.0 47 299046.5 48 308958.0 49 314787.0 50 311680.0 51 305873.5 52 298039.5 53 287069.5 54 275706.5 55 256067.0 56 231305.0 57 202920.0 58 176421.0 59 153534.0 60 126524.0 61 101318.5 62 81365.0 63 63380.0 64 46557.5 65 33328.0 66 24396.5 67 18521.0 68 14048.5 69 10519.5 70 8016.5 71 6101.5 72 4737.5 73 3683.5 74 2834.0 75 2072.5 76 1414.5 77 873.5 78 526.5 79 325.0 80 193.0 81 135.5 82 85.5 83 46.0 84 31.5 85 18.5 86 9.0 87 6.0 88 5.0 89 3.0 90 3.0 91 2.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028728586076104047 2 0.0016724622879985456 3 4.046279729028739E-5 4 0.019084952721918888 5 4.990411665802111E-4 6 1.3487599096762464E-5 7 4.046279729028739E-5 8 0.004005816931738452 9 0.010034773727991273 10-11 9.57619535870135E-4 12-13 8.766939412895601E-5 14-15 7.418179503219355E-5 16-17 3.3044617787068034E-4 18-19 3.371899774190616E-5 20-21 4.046279729028739E-5 22-23 3.1021477922553664E-4 24-25 4.046279729028739E-5 26-27 1.1464459232248095E-4 28-29 8.632063421927977E-4 30-31 0.0023400984432882872 32-33 0.009677352351927068 34-35 0.018794969341338494 36-37 0.021532951957981272 38-39 0.020757415009917433 40-41 0.004396957305544563 42-43 0.005024130663544018 44-45 0.008416261836379777 46-47 0.0112823766444418 48-49 1.2079223999078526 50-51 0.017533878825791202 52-53 0.017790143208629688 54-55 0.00925923677992743 56-57 0.007236096915413062 58-59 0.00799140246483176 60-61 0.004727403483415243 62-63 0.007290047311800111 64-65 0.01899728332778993 66-67 0.028229544909523837 68-69 0.028479065492813944 70-71 0.026206405045009467 72-73 0.02234220790378702 74-75 0.008807402210185888 76-77 0.013716888281407425 78-79 0.00815325365399291 80-81 0.0026031066256751553 82-83 0.013784326276891239 84-85 0.023070538255012193 86-87 0.02272660447804475 88-89 0.03164865128055312 90-91 0.020103266453724452 92-93 0.012725549747795386 94-95 0.006197551784962352 96-97 0.0034528253687711907 98-99 0.01567259015043798 100 0.03276137820603602 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7414218.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.768740667991736 #Duplication Level Percentage of deduplicated Percentage of total 1 74.37498471033705 44.452991733379854 2 15.03987654595609 17.97828961907722 3 5.002175366297496 8.969211667321549 4 2.0821072163075987 4.977797050177722 5 1.1190242172392988 3.344133412068905 6 0.6233162926210095 2.2352897908679505 7 0.3943704417023163 1.6499717260058968 8 0.25696230798845465 1.2286650838088453 9 0.186373488206889 1.0025377815623908 >10 0.8575486015425494 8.763121048870483 >50 0.042068072700928466 1.710415210288366 >100 0.019840903388691947 2.0813880673636227 >500 9.012287712737328E-4 0.34985410727655303 >1k 3.6048555236112967E-4 0.40380359233275515 >5k 0.0 0.0 >10k+ 9.012138803608776E-5 0.852530109597784 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20076 0.2707770394666032 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16700 0.22524290491593313 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10706 0.14439823592993895 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.3487599096762464E-5 2.6975198193524928E-5 5 0.0 2.6975198193524928E-5 0.0 1.3487599096762464E-5 2.6975198193524928E-5 6 0.0 2.6975198193524928E-5 0.0 1.3487599096762464E-5 2.6975198193524928E-5 7 0.0 2.6975198193524928E-5 0.0 1.3487599096762464E-5 2.6975198193524928E-5 8 0.0 2.6975198193524928E-5 0.0 1.3487599096762464E-5 2.6975198193524928E-5 9 0.0 2.6975198193524928E-5 0.0 1.3487599096762464E-5 6.743799548381232E-5 10-11 0.0 2.6975198193524928E-5 0.0 1.3487599096762464E-5 6.743799548381232E-5 12-13 0.0 4.046279729028739E-5 0.0 2.0231398645143697E-5 6.743799548381232E-5 14-15 0.0 4.046279729028739E-5 0.0 2.6975198193524928E-5 9.441319367733725E-5 16-17 0.0 4.046279729028739E-5 0.0 4.046279729028739E-5 9.441319367733725E-5 18-19 0.0 4.046279729028739E-5 0.0 5.3950396387049855E-5 1.2138839187086217E-4 20-21 0.0 5.3950396387049855E-5 0.0 9.441319367733725E-5 1.3487599096762465E-4 22-23 0.0 5.3950396387049855E-5 0.0 4.450907701931613E-4 1.483635900643871E-4 24-25 0.0 5.3950396387049855E-5 0.0 0.0010722641281926159 1.6185118916114957E-4 26-27 0.0 5.3950396387049855E-5 0.0 0.002056858862256276 1.6185118916114957E-4 28-29 0.0 5.3950396387049855E-5 0.0 0.005678279219736997 1.6185118916114957E-4 30-31 0.0 5.3950396387049855E-5 0.0 0.016947168265082035 1.6185118916114957E-4 32-33 0.0 5.3950396387049855E-5 0.0 0.044252812636477644 1.6185118916114957E-4 34-35 0.0 5.3950396387049855E-5 0.0 0.08528883288837744 1.6185118916114957E-4 36-37 0.0 6.0694195935431086E-5 0.0 0.14801965628741964 1.685949887095308E-4 38-39 0.0 6.743799548381232E-5 0.0 0.2358306702068917 1.9557018690305572E-4 40-41 0.0 6.743799548381232E-5 0.0 0.3292457815510685 2.1580158554819942E-4 42-43 0.0 6.743799548381232E-5 0.0 0.4261541810613068 2.1580158554819942E-4 44-45 0.0 6.743799548381232E-5 0.0 0.5413787401449486 2.1580158554819942E-4 46-47 0.0 6.743799548381232E-5 0.0 0.6799031266682474 2.2254538509658065E-4 48-49 0.0 6.743799548381232E-5 0.0 0.8295075758495367 2.8323958103201175E-4 50-51 0.0 6.743799548381232E-5 0.0 0.9851409818270787 2.8323958103201175E-4 52-53 0.0 6.743799548381232E-5 0.0 1.13923680150759 3.371899774190616E-4 54-55 0.0 8.092559458057479E-5 0.0 1.3100572440680867 3.978841733544927E-4 56-57 0.0 1.0790079277409971E-4 0.0 1.5103346030559124 4.046279729028739E-4 58-59 0.0 1.2138839187086217E-4 0.0 1.7385987301695203 4.046279729028739E-4 60-61 0.0 1.2138839187086217E-4 0.0 1.969850360483061 4.046279729028739E-4 62-63 0.0 1.281321914192434E-4 0.0 2.2052157085211146 4.046279729028739E-4 64-65 0.0 1.3487599096762465E-4 0.0 2.4484443268325804 4.046279729028739E-4 66-67 0.0 1.3487599096762465E-4 0.0 2.7232743898277603 4.046279729028739E-4 68-69 0.0 1.3487599096762465E-4 0.0 3.0239938992891764 4.046279729028739E-4 70-71 0.0 1.3487599096762465E-4 0.0 3.3410873540540624 4.046279729028739E-4 72-73 0.0 1.3487599096762465E-4 0.0 3.658221271616238 4.7206596838668624E-4 74-75 0.0 1.3487599096762465E-4 0.0 3.9687058028237097 4.7206596838668624E-4 76-77 0.0 1.3487599096762465E-4 0.0 4.309827415379477 4.788097679350675E-4 78-79 0.0 1.3487599096762465E-4 0.0 4.6864079259606335 4.855535674834487E-4 80-81 0.0 1.6185118916114957E-4 0.0 5.0817834058831295 5.057849661285924E-4 82-83 0.0 1.6185118916114957E-4 0.0 5.484253362930521 6.204295584510733E-4 84-85 0.0 1.685949887095308E-4 0.0 5.87556907552489 6.204295584510733E-4 86-87 0.0 1.888263873546745E-4 0.0 6.2859157904447915 6.204295584510733E-4 88 0.0 1.888263873546745E-4 0.0 6.612106630800443 6.204295584510733E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11010 0.0 49.69489 1 GTATCAA 19790 0.0 41.305023 1 CAACGCA 24355 0.0 33.034527 5 ATCAACG 24570 0.0 32.785194 3 TATCAAC 25390 0.0 31.945074 2 TCAACGC 25425 0.0 31.720228 4 AACGCAG 26345 0.0 30.71709 6 ACGCAGA 29670 0.0 26.97433 7 CGCAGAG 30575 0.0 26.129879 8 GTACATG 27475 0.0 25.089073 1 GTACTAG 2620 0.0 24.178703 1 TACATGG 28025 0.0 24.03701 2 ACATGGG 27790 0.0 23.415344 3 GCAGAGT 34135 0.0 23.08756 9 TACCTGG 5190 0.0 22.05434 2 GTGGTAT 5530 0.0 21.892488 1 CATGGGG 19495 0.0 21.51472 4 GAGTACT 19470 0.0 20.693659 12-13 AGAGTAC 31930 0.0 19.765448 10-11 CAGAGTA 33725 0.0 19.325396 10-11 >>END_MODULE