FastQCFastQC Report
Mon 6 Jun 2016
ERR1211290_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211290_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3449411
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151740.439901188927617No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118040.3422033500791874No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73810.2139785603977027No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66630.1931634125362272No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65800.050.326321
TACCTGG41250.045.7226072
GTACCTG44900.042.952251
GTATCAA119100.042.7725451
ACCTGGG47950.037.9242483
ATCAACG145450.034.179573
CAACGCA147650.033.7638055
TATCAAC149050.033.73312
TCAACGC148500.033.6041344
AACGCAG155400.032.563266
ACGCAGA169800.029.3883487
CGCAGAG174550.028.5873668
GTACATG131300.028.2299351
TACATGG134150.027.3473012
CCTGGGG50350.026.7841014
TATCACG2650.026.6229382
GCAGAGT187550.025.8808149
GTGGTAT30400.025.8399281
ACATGGG138250.025.7631363
ATAACGC4500.024.0165163
Produced by FastQC (version 0.11.5)