FastQCFastQC Report
Mon 6 Jun 2016
ERR1211290_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211290_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3449411
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140120.4062142783217193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112800.32701235080423874No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74910.21716751062717662No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55690.1614478529812771No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64200.050.2124441
TACCTGG43000.048.5518342
GTACCTG48250.043.1792761
ACCTGGG49650.040.919173
GTATCAA114000.040.3788381
ATCAACG143450.031.5961913
CAACGCA144850.031.383895
TCAACGC146700.030.8962084
TATCAAC147650.030.7896272
AACGCAG153750.029.7498446
CCTGGGG47350.028.8357454
ACGCAGA171350.026.6941287
GTACATG127700.026.2727071
CGCAGAG177350.025.711678
TACATGG130750.025.5836142
GTGGTAT30650.024.6481911
ACATGGG134000.024.3703983
CATGGGG88600.023.5659244
GCAGAGT192000.023.479689
CTGGGGG31400.023.0087265
Produced by FastQC (version 0.11.5)