##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211290_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3449411 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.115306642206455 33.0 31.0 34.0 30.0 34.0 2 32.22733301424504 33.0 31.0 34.0 30.0 34.0 3 32.29173850260233 34.0 31.0 34.0 30.0 34.0 4 35.93699822955281 37.0 35.0 37.0 35.0 37.0 5 35.845739461026824 37.0 35.0 37.0 35.0 37.0 6 35.866273401458976 37.0 35.0 37.0 35.0 37.0 7 35.78980063552879 37.0 35.0 37.0 33.0 37.0 8 35.770839137464336 37.0 35.0 37.0 33.0 37.0 9 37.39036113701731 39.0 37.0 39.0 34.0 39.0 10-11 37.3346751083011 39.0 37.0 39.0 34.0 39.0 12-13 37.25989683456103 39.0 37.0 39.0 34.0 39.0 14-15 38.48741277858741 40.0 38.0 41.0 34.0 41.0 16-17 38.47210813672247 40.0 38.0 41.0 34.0 41.0 18-19 38.482335679917526 40.0 38.0 41.0 34.0 41.0 20-21 38.44707951589416 40.0 38.0 41.0 34.0 41.0 22-23 38.325440343293394 40.0 38.0 41.0 34.0 41.0 24-25 38.2274065050526 40.0 38.0 41.0 33.5 41.0 26-27 38.12332641717673 40.0 37.5 41.0 33.0 41.0 28-29 38.005578923474175 40.0 37.0 41.0 33.0 41.0 30-31 37.992423779016185 40.0 37.0 41.0 33.0 41.0 32-33 37.899184672397695 40.0 37.0 41.0 33.0 41.0 34-35 37.73823762955473 40.0 37.0 41.0 33.0 41.0 36-37 37.571617589205815 40.0 36.5 41.0 32.5 41.0 38-39 37.44799952803537 40.0 36.0 41.0 32.0 41.0 40-41 37.331806792521974 40.0 36.0 41.0 31.5 41.0 42-43 37.2177887761128 40.0 36.0 41.0 31.0 41.0 44-45 37.05019567108704 39.0 36.0 41.0 31.0 41.0 46-47 36.79720465320021 39.0 35.0 41.0 30.5 41.0 48-49 35.241508767728746 39.0 35.0 40.5 23.0 41.0 50-51 35.96329141409939 39.0 35.0 40.0 28.0 41.0 52-53 36.05845403751539 39.0 35.0 40.0 29.0 41.0 54-55 35.884117027515714 38.0 35.0 40.0 28.0 41.0 56-57 35.66999351483485 38.0 34.5 40.0 28.0 41.0 58-59 35.43589035925264 38.0 34.0 40.0 28.0 41.0 60-61 35.17705950378195 37.5 34.0 40.0 27.0 41.0 62-63 34.85017152203666 37.0 34.0 40.0 26.5 41.0 64-65 34.513424030943256 36.0 33.0 39.0 26.0 41.0 66-67 34.17338568816531 36.0 33.0 39.0 26.0 40.0 68-69 33.80785922582145 35.5 33.0 39.0 26.0 40.0 70-71 33.43962548968505 35.0 32.0 38.0 25.5 40.0 72-73 33.055843591848 35.0 32.0 37.0 25.0 39.5 74-75 32.68968963107035 35.0 32.0 37.0 24.0 39.0 76-77 31.766423456062498 34.0 30.5 36.0 23.5 38.0 78-79 32.20157615314615 34.5 31.0 36.0 24.0 38.0 80-81 32.08080524472149 34.5 31.0 35.5 24.0 37.0 82-83 31.81381850408664 34.0 31.0 35.0 24.0 37.0 84-85 31.536461152353255 34.0 31.0 35.0 23.5 36.0 86-87 31.27989966982769 34.0 31.0 35.0 23.0 36.0 88-89 31.05672954020266 34.0 31.0 35.0 21.5 35.5 90-91 30.843102778996183 34.0 30.0 35.0 20.0 35.0 92-93 30.655415373813096 34.0 30.0 35.0 20.0 35.0 94-95 30.46271595353526 34.0 30.0 35.0 20.0 35.0 96-97 30.2754551429215 34.0 29.0 35.0 19.0 35.0 98-99 30.05596477195672 34.0 29.0 35.0 18.5 35.0 100 27.667821259919446 31.0 25.0 34.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.3018107169150497 1101 2 -0.2925514608119322 1101 3 -0.3172188915889862 1101 4 -0.13582289692592298 1101 5 -0.1782123006828158 1101 6 -0.2135744415190075 1101 7 -0.26689084276563335 1101 8 -0.2180725842901552 1101 9 -0.3112108575267527 1101 10-11 -0.3184971540405499 1101 12-13 -0.3619313930732133 1101 14-15 -0.4544842525125361 1101 16-17 -0.42775220022983973 1101 18-19 -0.4552184322495805 1101 20-21 -0.4950192005436307 1101 22-23 -0.6174341546158644 1101 24-25 -0.49131852862542047 1101 26-27 -0.5277398423514157 1101 28-29 -0.6098539467406141 1101 30-31 -0.5285380363607644 1101 32-33 -0.4241079787751332 1101 34-35 -0.517056133338464 1101 36-37 -0.5228030260581633 1101 38-39 -0.4876232330554089 1101 40-41 -0.5771529155887833 1101 42-43 -0.6264701598776412 1101 44-45 -0.6960548701072184 1101 46-47 -0.722403374402198 1101 48-49 0.6460175333124809 1101 50-51 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1103 28-29 -0.8192718191952011 1103 30-31 -0.7147992154071048 1103 32-33 -0.7782499453661202 1103 34-35 -0.6925814280677329 1103 36-37 -0.7543507185753242 1103 38-39 -0.6211190911191196 1103 40-41 -0.6480565176703905 1103 42-43 -0.6255894921256342 1103 44-45 -0.6326851235847926 1103 46-47 -0.6536105366682889 1103 48-49 0.4981040866198967 1103 50-51 -0.3838495272116731 1103 52-53 -0.69255276006934 1103 54-55 -0.6990236230776219 1103 56-57 -0.792460867840326 1103 58-59 -0.9450707328694179 1103 60-61 -0.9674012321268677 1103 62-63 -0.8114214812311218 1103 64-65 -0.9457538153553529 1103 66-67 -0.76920448554921 1103 68-69 -0.9008218130280525 1103 70-71 -0.826715742447135 1103 72-73 -0.7922277951084808 1103 74-75 -0.90140975675898 1103 76-77 -0.82447261940478 1103 78-79 -0.5069768425906105 1103 80-81 -0.708940685850294 1103 82-83 -0.9046179410190902 1103 84-85 -0.8252831816332851 1103 86-87 -0.8659580505014262 1103 88-89 -0.8050345834179247 1103 90-91 -0.9375393378362702 1103 92-93 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1105 70-71 -0.697599221005035 1105 72-73 -0.744676515301208 1105 74-75 -0.7759613492830866 1105 76-77 -0.7983042364294164 1105 78-79 -0.6469467478852593 1105 80-81 -0.5830041756450051 1105 82-83 -0.6332257070963969 1105 84-85 -0.6340962042201639 1105 86-87 -0.5887439133586412 1105 88-89 -0.607983155933205 1105 90-91 -0.6668889231584032 1105 92-93 -0.7021646591320838 1105 94-95 -0.7617394163939295 1105 96-97 -0.8135741374847889 1105 98-99 -0.8036715036049458 1105 100 -0.7325813678979927 1106 1 -0.25119938872290604 1106 2 -0.2115028091417095 1106 3 -0.24439896997729704 1106 4 -0.10744258158908337 1106 5 -0.23563903770332928 1106 6 -0.23349625249127826 1106 7 -0.2749643306867924 1106 8 -0.2169945581769923 1106 9 -0.31727905147180735 1106 10-11 -0.304564969346238 1106 12-13 -0.3465785634664016 1106 14-15 -0.4125464559843621 1106 16-17 -0.3757543046675309 1106 18-19 -0.4103803578698688 1106 20-21 -0.4752791862848724 1106 22-23 -0.5176406625296721 1106 24-25 -0.5806873318311077 1106 26-27 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1.1958127659777738 2311 1 0.37406248860327196 2311 2 0.4317694830527614 2311 3 0.44726110542409714 2311 4 0.28847377826589593 2311 5 0.3353482869924349 2311 6 0.40487535033045674 2311 7 0.4103171397609202 2311 8 0.1806113069115156 2311 9 0.49527821223635016 2311 10-11 0.44966421396588174 2311 12-13 0.5064465261681121 2311 14-15 0.44942020358916324 2311 16-17 0.4946337836781751 2311 18-19 0.6794531005971365 2311 20-21 0.6494242067780363 2311 22-23 0.7398193783428582 2311 24-25 0.6359043884690578 2311 26-27 0.7381533381515055 2311 28-29 0.7849637581896616 2311 30-31 0.6873915471514778 2311 32-33 0.764822706161489 2311 34-35 0.9081459500337559 2311 36-37 0.9313982545810404 2311 38-39 0.9398983753975543 2311 40-41 0.8772901183686912 2311 42-43 0.9600971296849679 2311 44-45 0.9214848465093581 2311 46-47 0.9989763929918993 2311 48-49 -5.84049188479942 2311 50-51 -0.8648265874515388 2311 52-53 0.547175059212698 2311 54-55 0.9904917457965183 2311 56-57 1.1110749255764816 2311 58-59 1.1598301968656628 2311 60-61 0.9683378103973936 2311 62-63 0.9067907282368353 2311 64-65 0.9161500007749268 2311 66-67 0.9995034415371649 2311 68-69 1.060804596592611 2311 70-71 1.0577802616944254 2311 72-73 0.576293810528206 2311 74-75 0.9147451024946633 2311 76-77 0.9797088278953083 2311 78-79 0.9395661578603409 2311 80-81 0.9484939388450684 2311 82-83 0.9973439286096131 2311 84-85 0.9958637738139906 2311 86-87 1.0638755218725322 2311 88-89 1.0513899657557957 2311 90-91 1.1596255480998359 2311 92-93 1.0795833290219328 2311 94-95 1.2053563467448178 2311 96-97 1.2446444389045226 2311 98-99 1.1522755382656236 2311 100 1.3018030490053256 2312 1 0.13345471023882993 2312 2 0.2884161879157574 2312 3 0.27425206798083224 2312 4 0.0347533652116212 2312 5 0.1821525337336709 2312 6 0.10025763751740868 2312 7 0.3146836779942177 2312 8 0.27652211409201044 2312 9 0.34123511279830154 2312 10-11 0.3037809843017598 2312 12-13 0.3507095849235995 2312 14-15 0.35831943213840134 2312 16-17 0.5162298577913518 2312 18-19 0.33092684568929087 2312 20-21 0.31047441617742066 2312 22-23 0.5915740346249407 2312 24-25 0.6563208122376736 2312 26-27 0.6289953127671097 2312 28-29 0.6632521071306172 2312 30-31 0.7123684023201164 2312 32-33 0.7079308132970326 2312 34-35 0.2748872690045374 2312 36-37 0.6071341620776067 2312 38-39 0.6968240940650148 2312 40-41 0.4504918712911561 2312 42-43 0.5621990075077292 2312 44-45 0.7498059744199637 2312 46-47 0.7285076602687397 2312 48-49 -4.682029153048511 2312 50-51 -0.8130071583551981 2312 52-53 0.5255696944534307 2312 54-55 0.7406334935764178 2312 56-57 0.785854910646016 2312 58-59 0.8129420398877656 2312 60-61 0.8266335395135584 2312 62-63 0.6841474281993527 2312 64-65 0.8307957229231064 2312 66-67 0.8155941535735849 2312 68-69 0.7565822923684422 2312 70-71 0.7500085708164619 2312 72-73 0.7803247078148843 2312 74-75 0.5617238432718992 2312 76-77 0.8170500936084437 2312 78-79 0.5329575642744118 2312 80-81 0.6224434252097382 2312 82-83 0.6730275925787232 2312 84-85 0.6515236746657855 2312 86-87 0.7088712658406422 2312 88-89 0.803003201393409 2312 90-91 0.6382461684925467 2312 92-93 0.7543487565650331 2312 94-95 0.9065827774552453 2312 96-97 0.9220387921545132 2312 98-99 0.9389534714763457 2312 100 0.5340569662884782 2313 1 0.05889941177402136 2313 2 0.18305899127489056 2313 3 0.22394018501290702 2313 4 0.055736316016044896 2313 5 0.11892879883171759 2313 6 0.20444667889427848 2313 7 0.18215505266210386 2313 8 0.20937930215471567 2313 9 0.3882039900768035 2313 10-11 0.18758651695043937 2313 12-13 -0.025351390951172448 2313 14-15 0.06817286372793063 2313 16-17 0.15518762615729997 2313 18-19 0.372358994892906 2313 20-21 0.3203752373554991 2313 22-23 0.4496955921602108 2313 24-25 0.2552027343868346 2313 26-27 0.4444503233487467 2313 28-29 0.34828166748257416 2313 30-31 0.5486421395286456 2313 32-33 0.5109199411417862 2313 34-35 0.4693355577371321 2313 36-37 -0.08236052308281927 2313 38-39 0.17203310578812392 2313 40-41 0.31000356790324446 2313 42-43 0.37664360572370015 2313 44-45 0.41993354886984235 2313 46-47 0.34942945829759253 2313 48-49 -5.120788689583669 2313 50-51 -1.3481125306112816 2313 52-53 0.1133258336545353 2313 54-55 0.2542089141256554 2313 56-57 0.2886865624686479 2313 58-59 0.42680820299715805 2313 60-61 0.6970887807524946 2313 62-63 0.7097635405069553 2313 64-65 0.7141840708793481 2313 66-67 0.7457933499712155 2313 68-69 0.7537451123959045 2313 70-71 0.7749254674732242 2313 72-73 0.6009867590482472 2313 74-75 0.703958184215395 2313 76-77 0.7240275338005304 2313 78-79 0.5823200351515538 2313 80-81 -0.4937193982489845 2313 82-83 0.03964315245259442 2313 84-85 0.487282531429134 2313 86-87 0.6925515954251296 2313 88-89 0.6133591608724487 2313 90-91 0.6418290768255268 2313 92-93 0.7163606447561044 2313 94-95 0.6450191269090446 2313 96-97 0.7440470432041941 2313 98-99 0.8692679012057454 2313 100 0.9038534045586779 2314 1 0.0780340322432167 2314 2 0.1841080196668088 2314 3 0.24383634721496605 2314 4 0.10055891216953228 2314 5 0.20303503185529337 2314 6 0.26146176300647284 2314 7 0.08159914581671757 2314 8 0.25377178549575774 2314 9 0.42436660921433145 2314 10-11 0.31215649723601047 2314 12-13 0.26290947116619634 2314 14-15 0.2864737356466662 2314 16-17 0.4773791756880499 2314 18-19 0.5433461540703988 2314 20-21 0.6541306770723025 2314 22-23 0.3486840636472692 2314 24-25 0.43917334222746973 2314 26-27 0.5209575242937277 2314 28-29 0.6827442796936083 2314 30-31 0.6194563970794533 2314 32-33 0.5958865859862854 2314 34-35 0.5047358903577148 2314 36-37 0.6024245061611282 2314 38-39 0.5461746490254527 2314 40-41 0.37968891896839096 2314 42-43 0.4508032472144379 2314 44-45 0.6168944946264716 2314 46-47 0.7618772954818311 2314 48-49 -6.53967611621313 2314 50-51 -1.5228020115042185 2314 52-53 -0.7580737869524583 2314 54-55 -0.29573425884821347 2314 56-57 0.04216526276082533 2314 58-59 0.49533597338916735 2314 60-61 0.7514021605752461 2314 62-63 0.5601552224603168 2314 64-65 0.8393854418779014 2314 66-67 0.8514348287847113 2314 68-69 0.8581206444510343 2314 70-71 0.5484893189305069 2314 72-73 0.8767127187211727 2314 74-75 0.8329933313214255 2314 76-77 0.6940105005883765 2314 78-79 0.5552171558907304 2314 80-81 0.5997668318103564 2314 82-83 0.6190918781642516 2314 84-85 0.6492473761304787 2314 86-87 0.6761870131856966 2314 88-89 0.5991998843259267 2314 90-91 0.8423150856839889 2314 92-93 0.8570592969203368 2314 94-95 0.8663611384666048 2314 96-97 0.9218117376081132 2314 98-99 0.9807583556804467 2314 100 -0.4665657077927463 2315 1 0.0196088652646651 2315 2 0.12801562790647836 2315 3 0.2329184485000937 2315 4 0.04482035808890572 2315 5 0.16697641882604586 2315 6 0.2645003896785383 2315 7 0.33238251325708035 2315 8 0.29270122588504677 2315 9 0.39276926919820454 2315 10-11 0.23514755645092578 2315 12-13 0.4273801555320489 2315 14-15 0.26004534400306767 2315 16-17 0.33709530302584056 2315 18-19 0.5088050304716205 2315 20-21 0.2841204267230282 2315 22-23 0.42442447811156825 2315 24-25 0.487303474241763 2315 26-27 0.5308841303337744 2315 28-29 0.5622260716443463 2315 30-31 0.5039092436488701 2315 32-33 0.6081642063002093 2315 34-35 0.5296449686652736 2315 36-37 0.4984874236075143 2315 38-39 0.5679311065631012 2315 40-41 0.5921967161646862 2315 42-43 0.6657797005935819 2315 44-45 0.7307828059452675 2315 46-47 0.6158964537858935 2315 48-49 -4.598015559630106 2315 50-51 -0.8242817460894827 2315 52-53 0.297159545286533 2315 54-55 0.05111147886687206 2315 56-57 0.4463272328706651 2315 58-59 0.566854046469409 2315 60-61 0.38718645820745934 2315 62-63 0.1536312946431977 2315 64-65 0.3943634273377299 2315 66-67 -0.33304409963428583 2315 68-69 0.379811441388334 2315 70-71 0.41758670454427715 2315 72-73 0.5575228610952436 2315 74-75 0.6960152874966212 2315 76-77 0.6385254693813494 2315 78-79 0.644267521219021 2315 80-81 0.4852374176852976 2315 82-83 0.5369641464096908 2315 84-85 0.594933292296485 2315 86-87 0.5837177368749487 2315 88-89 0.5087177531287495 2315 90-91 0.5553053059023476 2315 92-93 0.6961306371626215 2315 94-95 0.4420969931669916 2315 96-97 0.443643887226731 2315 98-99 0.3830043129937586 2315 100 0.5599265205456412 2316 1 0.1576514844804322 2316 2 0.1999918821927693 2316 3 0.08658104244463516 2316 4 -0.10534748042856279 2316 5 0.13800787613050147 2316 6 0.11166139360094718 2316 7 0.2213897824897515 2316 8 0.2803630908495478 2316 9 0.40580471834093146 2316 10-11 0.40706518954693394 2316 12-13 0.3089890553855952 2316 14-15 0.4092085580269966 2316 16-17 0.38327238293057775 2316 18-19 0.14730388045522602 2316 20-21 0.4010643633564044 2316 22-23 0.541076454798926 2316 24-25 0.5331122642114892 2316 26-27 0.5882045442057731 2316 28-29 0.5957071009429455 2316 30-31 0.5033799098018861 2316 32-33 0.4448317216140154 2316 34-35 0.4783238627266613 2316 36-37 0.307198107857765 2316 38-39 0.4338229348753302 2316 40-41 -0.05460696328458425 2316 42-43 -0.024463432889078263 2316 44-45 0.22543476079820834 2316 46-47 0.21488308949352586 2316 48-49 -5.1493357005115605 2316 50-51 -1.1095773693321362 2316 52-53 0.15423533526573152 2316 54-55 0.33533329508150445 2316 56-57 0.35489593911926676 2316 58-59 0.28833891476534745 2316 60-61 0.37020329200090885 2316 62-63 0.30488072096196817 2316 64-65 0.28636719902233665 2316 66-67 0.3819788666148227 2316 68-69 -0.05112647533917425 2316 70-71 -0.32973018023435685 2316 72-73 -0.21556635741293917 2316 74-75 0.05625166980135532 2316 76-77 0.176228593554832 2316 78-79 0.2692754754010025 2316 80-81 0.13883024402947797 2316 82-83 -0.008066415442357311 2316 84-85 0.23794206545008834 2316 86-87 0.28330051251080235 2316 88-89 0.034911575568727926 2316 90-91 0.03967219433311442 2316 92-93 0.16212135970634023 2316 94-95 0.44996383747655244 2316 96-97 0.5624540332827017 2316 98-99 0.381817172066647 2316 100 -0.5258001092835869 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 2.0 11 14.0 12 28.0 13 95.0 14 411.0 15 1135.0 16 2671.0 17 5560.0 18 9480.0 19 13498.0 20 17755.0 21 23257.0 22 29011.0 23 30889.0 24 31891.0 25 33953.0 26 38806.0 27 46896.0 28 56968.0 29 70205.0 30 85465.0 31 106008.0 32 132425.0 33 172652.0 34 238985.0 35 337818.0 36 516529.0 37 763537.0 38 608832.0 39 74627.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.28240926010344 18.61207483525827 13.51328779374254 23.592228110895746 2 16.232899329236528 20.28290522889365 39.72263783961804 23.761557602251784 3 18.822923681753203 26.147652454288572 30.101399920160283 24.928023943797943 4 12.905156408257143 16.679710232757337 37.32688761762044 33.08824574136507 5 13.260064446084025 36.766563411844686 34.03460606860044 15.938766073470855 6 30.507072223945542 35.986415068084106 18.77628348036331 14.730229227607039 7 27.6011339913591 32.14283954149827 22.15492566987562 18.101100797267012 8 26.43987337643606 34.503877477003165 20.414817125550005 18.64143202101077 9 27.03715816385404 16.159489999402744 20.497063866627006 36.30628797011621 10-11 25.364384957966283 25.732074863438992 28.048611139296582 20.854929039298145 12-13 25.688502400557546 23.677185672783757 28.21829955119822 22.41601237546048 14-15 23.206919799101673 25.007664361970676 26.312439545759535 25.472976293168117 16-17 22.467587097806632 27.477983114734805 27.086740932765657 22.967688854692906 18-19 22.105493708415892 26.548906414271777 29.25799328841647 22.087606588895866 20-21 23.08291852809611 25.98110981298251 29.038777646032223 21.89719401288916 22-23 22.967439311997286 25.738207024109954 28.87890810154215 22.41544556235061 24-25 22.475402322309517 25.842455422099597 29.139829959375675 22.542312296215208 26-27 22.37313047738599 25.948408568421844 29.031167648960256 22.64729330523191 28-29 22.37879325964849 26.20419641239355 29.0870809929335 22.329929335024463 30-31 22.906070077839946 25.92177910931179 29.121437360941854 22.05071345190641 32-33 22.74803379382658 26.15881602445166 28.801627906943033 22.291522274778735 34-35 22.860723818029197 26.221274387141015 28.826677143756008 22.091324651073784 36-37 22.81212218569801 26.102737508595624 28.70291905518642 22.382221250519944 38-39 22.74187636863007 26.28555538544334 28.650661233901197 22.321907012025388 40-41 22.790247358817926 25.922005600910687 28.903195337087475 22.384551703183913 42-43 22.615585506732927 26.18541227741855 28.522780215832867 22.676222000015656 44-45 22.392206969113687 26.49264322367389 28.581124589860696 22.534025217351722 46-47 22.461546936657186 26.113609412556066 28.279723746814305 23.145119903972443 48-49 22.685238506539324 26.31807982872654 28.161570077146123 22.835111587588013 50-51 22.54614621812678 26.550385349191895 28.061916829524304 22.841551603157022 52-53 22.596334163044745 26.717316322147234 27.769395609676963 22.91695390513106 54-55 22.495656848089418 26.526728069982152 27.989309119923178 22.98830596200525 56-57 22.675429975002565 26.568862973355206 27.679715083756772 23.07599196788546 58-59 22.45600678082171 26.566785715683576 27.980863052944077 22.996344450550637 60-61 22.70186256170946 26.743593059084525 27.82848799789402 22.72605638131199 62-63 22.698397373730522 26.721430868059997 27.74910405488191 22.831067703327573 64-65 22.48404211825331 26.402762611322427 27.793868879477472 23.31932639094679 66-67 22.41168443883865 26.413754375016584 27.741871592909845 23.432689593234922 68-69 22.159787786716663 26.483002710663662 27.828344450751686 23.528865051867996 70-71 22.27208253039308 26.251669736337885 27.928720798915823 23.54752693435321 72-73 22.249588752955134 26.605284317924337 27.535994446533824 23.609132482586702 74-75 22.301357002831313 26.630533658190224 27.43266847070772 23.635440868270745 76-77 22.176059951095183 26.501760176193034 27.550196879074164 23.771982993637618 78-79 22.01004602616606 26.449128402130974 27.628528974739968 23.912296596963 80-81 22.001828757766713 26.681758460179 27.35058283681265 23.965829945241634 82-83 22.245458085418353 26.542369215272792 27.55434944914816 23.657823250160703 84-85 22.168351816408112 26.662182728642055 27.29977377812568 23.869691676824154 86-87 21.830522678192008 26.702323312211433 27.49472435813326 23.972429651463298 88-89 21.838431298400604 26.35660893007113 27.558881119530064 24.2460786519982 90-91 22.10198522969736 26.320157958932594 27.490293931603226 24.087562879766825 92-93 21.907142178888993 26.483593349632827 27.419941968392123 24.189322503086057 94-95 21.557483084512782 26.269669019478687 27.604005095665496 24.568842800343035 96-97 21.528915790317484 26.240508764023314 27.681090702303262 24.549484743355933 98-99 21.511096897206407 26.25717186853053 27.42973685461882 24.801994379644245 100 21.45048293558017 26.241761849220165 27.558937976722348 24.74881723847732 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1803.0 1 1328.0 2 2616.5 3 3772.0 4 2230.0 5 1365.0 6 1842.0 7 3280.0 8 4917.5 9 5467.5 10 4622.5 11 3639.0 12 3503.5 13 3751.0 14 4165.5 15 4488.5 16 4797.5 17 5220.0 18 5559.5 19 5813.5 20 6248.5 21 6889.0 22 7597.0 23 8714.5 24 10136.5 25 11833.5 26 14284.5 27 17261.0 28 20763.0 29 25282.0 30 31065.0 31 36702.0 32 43480.5 33 51534.5 34 58881.0 35 66059.0 36 74633.5 37 84136.0 38 89922.5 39 92871.0 40 95597.5 41 98060.5 42 101413.5 43 106329.0 44 111832.5 45 118540.5 46 125772.5 47 131166.5 48 136408.5 49 140821.5 50 142220.5 51 140992.0 52 137013.0 53 132109.0 54 127378.5 55 119338.5 56 109188.5 57 98396.0 58 87198.0 59 76206.0 60 63205.5 61 50441.5 62 40717.0 63 32077.5 64 23623.0 65 17349.0 66 12923.0 67 9762.5 68 7545.5 69 5747.0 70 4437.0 71 3437.0 72 2814.0 73 2304.0 74 1764.0 75 1285.0 76 904.0 77 553.0 78 329.5 79 223.0 80 163.0 81 105.0 82 57.5 83 34.5 84 17.5 85 12.0 86 9.0 87 4.5 88 2.5 89 1.0 90 0.5 91 1.0 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028497618868844568 2 0.001681446484631724 3 0.0 4 0.02064120512168599 5 4.638473061053032E-4 6 2.899045663158145E-5 7 5.79809132631629E-5 8 0.0038267402753687516 9 0.00918997475221132 10-11 0.0010726468953685137 12-13 1.159618265263258E-4 14-15 1.3045705484211653E-4 16-17 3.768759362105589E-4 18-19 4.348568494737217E-5 20-21 2.899045663158145E-5 22-23 1.7394273978948868E-4 24-25 0.0 26-27 2.899045663158145E-5 28-29 0.0010436564387369322 30-31 0.0025946458685265398 32-33 0.009262450893790273 34-35 0.017858121285054172 36-37 0.02056872898010704 38-39 0.020496252838528087 40-41 0.0043630637230530084 42-43 0.004870396714105683 44-45 0.008059346943579643 46-47 0.011625173109264161 48-49 1.2039011877679986 50-51 0.017133359869264637 52-53 0.018017568796527873 54-55 0.008784108359369179 56-57 0.007450547354316432 58-59 0.007870908975474364 60-61 0.005305253563579406 62-63 0.007537518724211176 64-65 0.018698844527370036 66-67 0.028859999576739334 68-69 0.02880201866347617 70-71 0.025729030260528536 72-73 0.021858804300212413 74-75 0.008769613131053388 76-77 0.013524048018632745 78-79 0.008291270596632296 80-81 0.002174284247368609 82-83 0.013915419183159096 84-85 0.02335181281673886 86-87 0.02256907048768616 88-89 0.03209243549116066 90-91 0.019322139344949037 92-93 0.012422410666632652 94-95 0.005943043609474197 96-97 0.0032034454577897498 98-99 0.015046046991790771 100 0.032614263710529126 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3449411.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.03651413635482 #Duplication Level Percentage of deduplicated Percentage of total 1 79.62677993287672 51.78648198736951 2 12.889864115306382 16.766236595016327 3 3.5186023285061054 6.86512890234295 4 1.3749264455979955 3.5768169286232854 5 0.7192376155172777 2.3388353674493785 6 0.4467788983503418 1.7434165283032224 7 0.28114742448920355 1.2799393913034287 8 0.2015962023396673 1.0488891410639376 9 0.15215970681468066 0.8906343230912925 >10 0.735977466188603 8.374593109132512 >50 0.03585835409725447 1.5918660581142643 >100 0.015941446621642087 1.7970507405706955 >500 6.677646741009237E-4 0.28275676113796544 >1k 2.5683256669877924E-4 0.3761292971399687 >5k 1.027330266795117E-4 0.43629528560689146 >10k+ 1.027330266795117E-4 0.8449295837342647 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14012 0.4062142783217193 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11280 0.32701235080423874 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7491 0.21716751062717662 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5569 0.1614478529812771 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.899045663158145E-5 2 0.0 0.0 0.0 0.0 2.899045663158145E-5 3 0.0 0.0 0.0 0.0 2.899045663158145E-5 4 0.0 0.0 0.0 0.0 2.899045663158145E-5 5 0.0 0.0 0.0 0.0 2.899045663158145E-5 6 0.0 0.0 0.0 0.0 1.4495228315790725E-4 7 0.0 5.79809132631629E-5 0.0 0.0 1.4495228315790725E-4 8 0.0 5.79809132631629E-5 0.0 0.0 1.4495228315790725E-4 9 2.899045663158145E-5 5.79809132631629E-5 0.0 5.79809132631629E-5 1.739427397894887E-4 10-11 2.899045663158145E-5 5.79809132631629E-5 0.0 7.247614157895363E-5 1.739427397894887E-4 12-13 2.899045663158145E-5 5.79809132631629E-5 0.0 8.697136989474436E-5 1.739427397894887E-4 14-15 2.899045663158145E-5 5.79809132631629E-5 0.0 8.697136989474436E-5 2.319236530526516E-4 16-17 2.899045663158145E-5 5.79809132631629E-5 0.0 1.0146659821053507E-4 2.6091410968423305E-4 18-19 2.899045663158145E-5 5.79809132631629E-5 0.0 1.3045705484211653E-4 2.899045663158145E-4 20-21 2.899045663158145E-5 5.79809132631629E-5 0.0 1.7394273978948868E-4 3.043997946316052E-4 22-23 2.899045663158145E-5 1.0146659821053507E-4 0.0 3.623807078947681E-4 3.333902512631867E-4 24-25 2.899045663158145E-5 1.4495228315790725E-4 0.0 0.0010871421236843043 3.478854795789774E-4 26-27 2.899045663158145E-5 1.4495228315790725E-4 0.0 0.002304741302210725 3.478854795789774E-4 28-29 2.899045663158145E-5 1.4495228315790725E-4 0.0 0.005885062696211034 3.478854795789774E-4 30-31 2.899045663158145E-5 1.4495228315790725E-4 0.0 0.016727493476422498 3.478854795789774E-4 32-33 2.899045663158145E-5 1.4495228315790725E-4 0.0 0.043674122915477454 3.768759362105588E-4 34-35 2.899045663158145E-5 1.4495228315790725E-4 0.0 0.08323160098927035 3.768759362105588E-4 36-37 2.899045663158145E-5 1.4495228315790725E-4 0.0 0.1417198472434859 3.913711645263495E-4 38-39 2.899045663158145E-5 1.4495228315790725E-4 0.0 0.229126073987704 4.2036162115793104E-4 40-41 2.899045663158145E-5 1.4495228315790725E-4 0.0 0.33246255665097607 4.928377627368846E-4 42-43 5.79809132631629E-5 1.4495228315790725E-4 0.0 0.4327115556829847 5.363234476842569E-4 44-45 5.79809132631629E-5 1.4495228315790725E-4 0.0 0.5538046930331004 5.798091326316289E-4 46-47 5.79809132631629E-5 1.4495228315790725E-4 0.0 0.6938430937919546 6.232948175790012E-4 48-49 5.79809132631629E-5 1.4495228315790725E-4 0.0 0.8444920016779676 7.537518724211176E-4 50-51 5.79809132631629E-5 1.739427397894887E-4 0.0 1.0112885939077714 7.537518724211176E-4 52-53 5.79809132631629E-5 1.739427397894887E-4 0.0 1.18137560296526 7.537518724211176E-4 54-55 5.79809132631629E-5 1.739427397894887E-4 0.0 1.378568688973277 7.537518724211176E-4 56-57 5.79809132631629E-5 1.739427397894887E-4 0.0 1.5888944518354005 7.82742329052699E-4 58-59 5.79809132631629E-5 1.739427397894887E-4 0.0 1.828573052037 8.117327856842806E-4 60-61 5.79809132631629E-5 2.319236530526516E-4 0.0 2.0703244698877574 8.117327856842806E-4 62-63 5.79809132631629E-5 2.319236530526516E-4 0.0 2.32297629943199 8.117327856842806E-4 64-65 5.79809132631629E-5 2.319236530526516E-4 0.0 2.571960836212327 8.407232423158621E-4 66-67 1.159618265263258E-4 2.6091410968423305E-4 0.0 2.857212434238773 8.697136989474435E-4 68-69 1.159618265263258E-4 2.6091410968423305E-4 0.0 3.175281229172169 8.697136989474435E-4 70-71 1.159618265263258E-4 2.754093380000238E-4 0.0 3.4988582108655653 9.276946122106064E-4 72-73 1.159618265263258E-4 2.899045663158145E-4 0.0 3.810157734175487 0.0010436564387369322 74-75 1.159618265263258E-4 2.899045663158145E-4 0.0 4.133299859019409 0.0010436564387369322 76-77 1.159618265263258E-4 2.899045663158145E-4 0.0 4.484562726795966 0.0010436564387369322 78-79 1.159618265263258E-4 2.899045663158145E-4 0.0 4.877484880752105 0.0010726468953685137 80-81 1.159618265263258E-4 2.899045663158145E-4 0.0 5.279799942656878 0.0010871421236843043 82-83 1.159618265263258E-4 2.899045663158145E-4 0.0 5.67498335223028 0.001101637352000095 84-85 1.159618265263258E-4 2.899045663158145E-4 0.0 6.084401076009788 0.0011161325803158858 86-87 1.159618265263258E-4 2.899045663158145E-4 0.0 6.504617744884562 0.0011306278086316765 88 1.159618265263258E-4 2.899045663158145E-4 0.0 6.834674093635116 0.0011306278086316765 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6420 0.0 50.212444 1 TACCTGG 4300 0.0 48.551834 2 GTACCTG 4825 0.0 43.179276 1 ACCTGGG 4965 0.0 40.91917 3 GTATCAA 11400 0.0 40.378838 1 ATCAACG 14345 0.0 31.596191 3 CAACGCA 14485 0.0 31.38389 5 TCAACGC 14670 0.0 30.896208 4 TATCAAC 14765 0.0 30.789627 2 AACGCAG 15375 0.0 29.749844 6 CCTGGGG 4735 0.0 28.835745 4 ACGCAGA 17135 0.0 26.694128 7 GTACATG 12770 0.0 26.272707 1 CGCAGAG 17735 0.0 25.71167 8 TACATGG 13075 0.0 25.583614 2 GTGGTAT 3065 0.0 24.648191 1 ACATGGG 13400 0.0 24.370398 3 CATGGGG 8860 0.0 23.565924 4 GCAGAGT 19200 0.0 23.47968 9 CTGGGGG 3140 0.0 23.008726 5 >>END_MODULE