FastQCFastQC Report
Mon 6 Jun 2016
ERR1211289_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211289_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7755902
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318250.4103326731049464No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251910.3247978120404306No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152150.19617318527232552No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141300.050.8290941
GTATCAA259450.041.9130861
ATCAACG323500.032.812963
TATCAAC329050.032.689732
CAACGCA324650.032.666945
TCAACGC338950.031.3861924
AACGCAG344850.031.1481766
GTACATG323000.028.6429231
TACCTGG59050.028.2758432
ACGCAGA386450.027.6254947
TACATGG332550.027.2258152
CGCAGAG393550.027.042488
ACATGGG331700.026.5335733
GCAGAGT424550.024.504869
CATGGGG229100.024.0176014
GTACCTG72800.023.8402331
ATGGGGG156200.023.0129685
GTGGTAT67500.022.1584221
GAGTACT253050.021.87443412-13
ACCTGGG73300.021.7319763
Produced by FastQC (version 0.11.5)