##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211289_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7755902 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.336072064861057 31.0 31.0 34.0 28.0 34.0 2 31.345249205057 31.0 31.0 34.0 28.0 34.0 3 31.39304686933899 31.0 31.0 34.0 28.0 34.0 4 34.99935777940464 37.0 35.0 37.0 32.0 37.0 5 35.04962685191226 37.0 35.0 37.0 32.0 37.0 6 35.116340304454596 37.0 35.0 37.0 32.0 37.0 7 35.050742905209475 37.0 35.0 37.0 32.0 37.0 8 35.05032129080538 37.0 35.0 37.0 32.0 37.0 9 36.60081741620768 39.0 37.0 39.0 32.0 39.0 10-11 36.529735999758636 39.0 37.0 39.0 32.0 39.0 12-13 36.4419337041649 39.0 37.0 39.0 32.0 39.0 14-15 37.55803386891686 40.0 37.0 41.0 32.0 41.0 16-17 37.55549773578882 40.0 37.0 41.0 32.0 41.0 18-19 37.568465086330384 40.0 37.0 41.0 32.0 41.0 20-21 37.53901525573686 40.0 37.0 41.0 31.5 41.0 22-23 37.41696633093095 40.0 37.0 41.0 31.0 41.0 24-25 37.3039137807569 40.0 37.0 41.0 31.0 41.0 26-27 37.15849510476022 40.0 36.0 41.0 30.5 41.0 28-29 37.018334940281605 40.0 36.0 41.0 30.0 41.0 30-31 36.825190093943945 39.0 36.0 41.0 30.0 41.0 32-33 36.68690475975586 39.0 35.5 41.0 30.0 41.0 34-35 36.53405741846661 39.0 35.0 41.0 30.0 41.0 36-37 36.43158055632988 39.0 35.0 41.0 29.0 41.0 38-39 36.27489046406208 39.0 35.0 41.0 29.0 41.0 40-41 36.10778778535365 39.0 35.0 40.0 28.0 41.0 42-43 35.95786570021127 39.0 35.0 40.0 27.0 41.0 44-45 35.813851567490154 38.5 35.0 40.0 27.0 41.0 46-47 35.6338877154456 38.0 34.5 40.0 26.0 41.0 48-49 35.390177312709724 38.0 34.0 40.0 25.5 41.0 50-51 34.717978901744765 37.5 33.5 39.5 24.0 40.5 52-53 34.95577187540533 38.0 33.5 40.0 25.5 40.5 54-55 35.31480780700942 38.0 34.0 40.0 26.0 41.0 56-57 35.21911661596549 38.0 34.0 40.0 26.0 41.0 58-59 34.99886344618589 38.0 34.0 40.0 25.0 41.0 60-61 34.73796645444979 37.5 33.5 40.0 25.0 41.0 62-63 34.46649699802808 37.0 33.0 40.0 24.5 41.0 64-65 34.19679651702665 36.5 33.0 40.0 24.0 41.0 66-67 33.976332604512024 36.0 33.0 39.0 23.5 41.0 68-69 33.64127454163294 36.0 32.5 39.0 23.0 41.0 70-71 33.300204283138186 35.0 32.0 39.0 23.0 40.5 72-73 32.93995456621293 35.0 32.0 38.0 22.0 40.0 74-75 32.57140310695004 35.0 31.0 37.0 21.5 39.5 76-77 32.21813897339084 35.0 31.0 37.0 20.5 39.0 78-79 31.85323099750358 35.0 31.0 36.0 20.0 39.0 80-81 31.516879403581942 35.0 30.5 36.0 20.0 37.5 82-83 31.20293777564492 34.0 30.0 35.0 19.5 37.0 84-85 30.898615596225945 34.0 30.0 35.0 18.5 37.0 86-87 30.63949267280582 34.0 30.0 35.0 18.0 36.0 88-89 30.379468113444446 34.0 29.0 35.0 18.0 36.0 90-91 30.11893594065526 34.0 29.0 35.0 17.0 36.0 92-93 29.87987148367785 34.0 29.0 35.0 16.0 35.0 94-95 29.654729391887624 34.0 29.0 35.0 15.0 35.0 96-97 29.45296904215654 34.0 28.5 35.0 14.0 35.0 98-99 29.219485947604802 34.0 27.0 35.0 11.0 35.0 100 27.554325982974 32.0 24.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.332483929371687 1101 2 -0.43444506270286 1101 3 -0.4035318945436366 1101 4 -0.2574571818242859 1101 5 -0.2979235188789744 1101 6 -0.2813628705293567 1101 7 -0.38904083221046193 1101 8 -0.41538611117789515 1101 9 -0.46834253247252633 1101 10-11 -0.48535599238514493 1101 12-13 -0.5055743775338968 1101 14-15 -0.5452526316088395 1101 16-17 -0.5920214911643242 1101 18-19 -0.6082889327535526 1101 20-21 -0.5772687406129009 1101 22-23 -0.614913878573006 1101 24-25 -0.6844519000366631 1101 26-27 -0.7844014350236534 1101 28-29 -0.7771761287675929 1101 30-31 -0.8080480837312578 1101 32-33 -0.7100079129809771 1101 34-35 -0.6899639988624173 1101 36-37 -0.8469707353209159 1101 38-39 -0.7985400543103438 1101 40-41 -0.8181029776363573 1101 42-43 -0.9107350949032664 1101 44-45 -0.9894353558115796 1101 46-47 -0.9277432849941505 1101 48-49 -0.8676792163457918 1101 50-51 -0.8156703672399672 1101 52-53 -0.634890372754235 1101 54-55 -0.6226508124219308 1101 56-57 -0.5360742070639901 1101 58-59 -0.645847153530795 1101 60-61 -0.6688974061266606 1101 62-63 -0.6922412428532212 1101 64-65 -0.7077901895382581 1101 66-67 -0.7699726632557429 1101 68-69 -0.790113253579392 1101 70-71 -0.7247140719295757 1101 72-73 -0.8282313788973781 1101 74-75 -0.8373479688094889 1101 76-77 -0.8431861169609611 1101 78-79 -0.8685473356517441 1101 80-81 -0.9225226842571921 1101 82-83 -1.0165703482041657 1101 84-85 -0.9460029903518468 1101 86-87 -0.9746381893168454 1101 88-89 -0.9906337492735737 1101 90-91 -1.1251686140887642 1101 92-93 -1.0607724831336007 1101 94-95 -1.0831703098968397 1101 96-97 -1.1287680971960796 1101 98-99 -1.2948292943555266 1101 100 -1.2913462368094102 1102 1 -0.33213188135335514 1102 2 -0.2867422512798363 1102 3 -0.29811745857323757 1102 4 -0.14586945386244565 1102 5 -0.2169232122590259 1102 6 -0.24098999987742786 1102 7 -0.329322444040713 1102 8 -0.2968432162282042 1102 9 -0.3201052374867359 1102 10-11 -0.3211090547630917 1102 12-13 -0.3918640111330305 1102 14-15 -0.5083541093621591 1102 16-17 -0.5279328011789843 1102 18-19 -0.5187384626922409 1102 20-21 -0.6124667692847368 1102 22-23 -0.5537443234510633 1102 24-25 -0.5932439015056872 1102 26-27 -0.6362389184773036 1102 28-29 -0.6334259213211979 1102 30-31 -0.5964763631054595 1102 32-33 -0.6354938027752013 1102 34-35 -0.6529204351778759 1102 36-37 -0.726840252368504 1102 38-39 -0.836687174180291 1102 40-41 -0.8754704239339901 1102 42-43 -0.7790222419213748 1102 44-45 -0.7600407888761893 1102 46-47 -0.8107645212108707 1102 48-49 -0.7531588951607162 1102 50-51 -0.681508923531112 1102 52-53 -0.6128997744106144 1102 54-55 -0.5063580829113548 1102 56-57 -0.4474083920368628 1102 58-59 -0.5213791496915405 1102 60-61 -0.6743526159427802 1102 62-63 -0.6724147331331238 1102 64-65 -0.6177574469223401 1102 66-67 -0.5544705444583613 1102 68-69 -0.60665662329545 1102 70-71 -0.6617940210767159 1102 72-73 -0.6390629210080476 1102 74-75 -0.7005455199001389 1102 76-77 -0.7033048312276478 1102 78-79 -0.6712715207485438 1102 80-81 -0.7047354591082744 1102 82-83 -0.7805606211232856 1102 84-85 -0.747093768425664 1102 86-87 -0.8128127400021228 1102 88-89 -0.774470509318391 1102 90-91 -0.8152517419890799 1102 92-93 -0.8380339303336122 1102 94-95 -0.8771010743860224 1102 96-97 -0.8677432570710337 1102 98-99 -0.8397756127992047 1102 100 -0.7951578554261154 1103 1 -0.4043801554797959 1103 2 -0.38884565660530157 1103 3 -0.30805548756026724 1103 4 -0.2418248643898835 1103 5 -0.18681165101143904 1103 6 -0.35037102306630175 1103 7 -0.3248844779508886 1103 8 -0.3571453218786189 1103 9 -0.4143665895263666 1103 10-11 -0.5113437356088397 1103 12-13 -0.5511341994493151 1103 14-15 -0.6851538422968702 1103 16-17 -0.5898714879539355 1103 18-19 -0.6592227148783749 1103 20-21 -0.7384666487751517 1103 22-23 -0.6781080461464697 1103 24-25 -0.7425469846184569 1103 26-27 -0.7097794013851342 1103 28-29 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-0.8890008249539569 1103 94-95 -1.075731452468645 1103 96-97 -1.1627705894860405 1103 98-99 -1.2294839302250047 1103 100 -1.2623434886775655 1104 1 -0.3996156724068811 1104 2 -0.4982196958702012 1104 3 -0.4849685368597605 1104 4 -0.3639314357170491 1104 5 -0.2882904289688497 1104 6 -0.28409552064729837 1104 7 -0.3285606808663317 1104 8 -0.36708669360304924 1104 9 -0.4307379841142094 1104 10-11 -0.4351355735791387 1104 12-13 -0.535671675359886 1104 14-15 -0.5473265321924998 1104 16-17 -0.5580721183130279 1104 18-19 -0.6282804839386316 1104 20-21 -0.6033973311610268 1104 22-23 -0.6167382654245799 1104 24-25 -0.7561561755391395 1104 26-27 -0.7992325841182861 1104 28-29 -0.6901155311128022 1104 30-31 -0.738702393311101 1104 32-33 -0.7476981906945568 1104 34-35 -0.7396978805738854 1104 36-37 -0.7330710937181735 1104 38-39 -0.7565570538600497 1104 40-41 -0.7974744567543439 1104 42-43 -0.8980242447873721 1104 44-45 -0.8678637395853528 1104 46-47 -0.8516494668128658 1104 48-49 -0.7880461501242948 1104 50-51 -0.7981410927403445 1104 52-53 -0.6841005007681957 1104 54-55 -0.7092600045939648 1104 56-57 -0.6174863287093117 1104 58-59 -0.6591588726514317 1104 60-61 -0.603087126579851 1104 62-63 -0.7069030475945652 1104 64-65 -0.6936164587159368 1104 66-67 -0.6968128191989891 1104 68-69 -0.6294044051333287 1104 70-71 -0.610457896836671 1104 72-73 -0.6840066174756743 1104 74-75 -0.7224196238870313 1104 76-77 -0.7250328584418071 1104 78-79 -0.7553551423207132 1104 80-81 -0.7959436631799299 1104 82-83 -0.8840311008883326 1104 84-85 -0.8442715725713619 1104 86-87 -0.7556691178017836 1104 88-89 -0.8040997107551142 1104 90-91 -0.8529379438999669 1104 92-93 -0.8220971714083305 1104 94-95 -0.8837072998622411 1104 96-97 -0.936564458673093 1104 98-99 -0.9977121206121566 1104 100 -0.9946082377983423 1105 1 -0.3957862135443655 1105 2 -0.450006609155686 1105 3 -0.3958664317802132 1105 4 -0.28507272600858613 1105 5 -0.3064408123480362 1105 6 -0.35407559746559514 1105 7 -0.3310248232546371 1105 8 -0.27286846044911073 1105 9 -0.41602049932607343 1105 10-11 -0.4605595624543142 1105 12-13 -0.4803010249590969 1105 14-15 -0.5487751158374152 1105 16-17 -0.6206427524580747 1105 18-19 -0.5619079224192589 1105 20-21 -0.5433728007336498 1105 22-23 -0.5465695341040444 1105 24-25 -0.5234128490215113 1105 26-27 -0.5864397531555667 1105 28-29 -0.6258693046776997 1105 30-31 -0.6931867889819756 1105 32-33 -0.5986259355651313 1105 34-35 -0.6777767442101776 1105 36-37 -0.7910154090030375 1105 38-39 -0.6631314367194321 1105 40-41 -0.7943229372932024 1105 42-43 -0.7186228254407254 1105 44-45 -0.7952735233601871 1105 46-47 -0.7598387599110907 1105 48-49 -0.727653768149402 1105 50-51 -0.7357435518216064 1105 52-53 -0.6399595912283331 1105 54-55 -0.6761375468296222 1105 56-57 -0.635058550836554 1105 58-59 -0.5261024845231006 1105 60-61 -0.5930418728461717 1105 62-63 -0.5998677838636794 1105 64-65 -0.5896526228442127 1105 66-67 -0.5941727997680673 1105 68-69 -0.5801182630856516 1105 70-71 -0.5359109861881919 1105 72-73 -0.6090812205869653 1105 74-75 -0.616635455084328 1105 76-77 -0.6611906711876436 1105 78-79 -0.5524408131100422 1105 80-81 -0.5678728941770892 1105 82-83 -0.5906934222690232 1105 84-85 -0.6783835039091137 1105 86-87 -0.6597163118574052 1105 88-89 -0.678190212049401 1105 90-91 -0.6782910532902164 1105 92-93 -0.722345277305255 1105 94-95 -0.6996094023617623 1105 96-97 -0.7575729251151841 1105 98-99 -0.7743935555529262 1105 100 -0.6951429679545384 1106 1 -0.4796854467771787 1106 2 -0.5590058377130909 1106 3 -0.5486161570998931 1106 4 -0.42515998084179074 1106 5 -0.4384722194125814 1106 6 -0.4479722007804554 1106 7 -0.46601856989418877 1106 8 -0.4736185130474695 1106 9 -0.5876378988632069 1106 10-11 -0.6030151168410143 1106 12-13 -0.6151814221351515 1106 14-15 -0.7548882432189572 1106 16-17 -0.7966528162774154 1106 18-19 -0.7906460001245676 1106 20-21 -0.8128654311191426 1106 22-23 -0.8145779849070536 1106 24-25 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0.2662847440639453 2311 7 0.410527197926271 2311 8 0.4017814274390048 2311 9 0.5587210732806227 2311 10-11 0.6004155259050492 2311 12-13 0.5824907351341366 2311 14-15 0.7413032991412365 2311 16-17 0.7283529910907376 2311 18-19 0.6468541438597697 2311 20-21 0.728833824001903 2311 22-23 0.7615374377349653 2311 24-25 0.7801827279429858 2311 26-27 0.8954184696493854 2311 28-29 0.8403336336670932 2311 30-31 0.8872701144497839 2311 32-33 0.8910848696135574 2311 34-35 0.8697765881097155 2311 36-37 0.8959588686037208 2311 38-39 0.8989350798313254 2311 40-41 0.9158789274377952 2311 42-43 0.9755939907084539 2311 44-45 1.009254735801953 2311 46-47 0.9618767419321088 2311 48-49 0.9619621853083302 2311 50-51 0.9222194005846802 2311 52-53 0.7732926510093421 2311 54-55 0.7463876783442416 2311 56-57 0.7389636371820387 2311 58-59 0.7111744793350709 2311 60-61 0.7195325517094915 2311 62-63 0.7350989309414189 2311 64-65 0.6794709250852051 2311 66-67 0.7220029023563583 2311 68-69 0.792089232575556 2311 70-71 0.7284062397710827 2311 72-73 0.7056673510949665 2311 74-75 0.6585750713981469 2311 76-77 0.7219425867710356 2311 78-79 0.7482636865669257 2311 80-81 0.7042597018780974 2311 82-83 0.7160485715489919 2311 84-85 0.8411857996037249 2311 86-87 0.8725930504349968 2311 88-89 0.8651019185831146 2311 90-91 0.8607608570456122 2311 92-93 0.8688996238209263 2311 94-95 0.9643245371358269 2311 96-97 0.9039224225583311 2311 98-99 0.99971327060398 2311 100 1.0717189444081718 2312 1 0.44487550159213995 2312 2 0.4620933659694977 2312 3 0.4889708364604175 2312 4 0.3362404388970148 2312 5 0.4034379589803123 2312 6 0.34961173332257545 2312 7 0.4551114781127552 2312 8 0.4387851199019437 2312 9 0.5255121948326718 2312 10-11 0.5609737130180363 2312 12-13 0.5788367067101419 2312 14-15 0.7592655553860226 2312 16-17 0.7740266017700961 2312 18-19 0.7034032696251273 2312 20-21 0.7239322068549754 2312 22-23 0.7481747149469271 2312 24-25 0.6919757839091432 2312 26-27 0.8394807276493736 2312 28-29 0.8159004212306868 2312 30-31 0.8065553375983896 2312 32-33 0.881556452705496 2312 34-35 0.9080165821293704 2312 36-37 0.9059631571982152 2312 38-39 0.9291193149325849 2312 40-41 0.9862704937729205 2312 42-43 0.9504217067865781 2312 44-45 0.9534863409458936 2312 46-47 0.9471450471252396 2312 48-49 0.9550624489680715 2312 50-51 0.9474622755543933 2312 52-53 0.8377602129095365 2312 54-55 0.7480987017238547 2312 56-57 0.7626632631142485 2312 58-59 0.7407630454726828 2312 60-61 0.8101753825244415 2312 62-63 0.7795492982844934 2312 64-65 0.760270567152503 2312 66-67 0.7377782238846393 2312 68-69 0.7598128670240527 2312 70-71 0.7881356369655705 2312 72-73 0.7804189488501834 2312 74-75 0.8044575316599207 2312 76-77 0.855477257244992 2312 78-79 0.8252791938116495 2312 80-81 0.8628358758173924 2312 82-83 0.7977544118195716 2312 84-85 0.8114106119354219 2312 86-87 0.9142653078174909 2312 88-89 0.9719558824244245 2312 90-91 0.9977869229453233 2312 92-93 0.9876740908023471 2312 94-95 0.9767628357226634 2312 96-97 1.0092150963997284 2312 98-99 1.0869355481606284 2312 100 1.1432329229423601 2313 1 0.4850939397795031 2313 2 0.5303179521872821 2313 3 0.5485332478301039 2313 4 0.43525417997643956 2313 5 0.47340712333170387 2313 6 0.456368527202649 2313 7 0.4960257608162095 2313 8 0.5218447071987384 2313 9 0.5540365715239091 2313 10-11 0.6193609794858261 2313 12-13 0.651653939700644 2313 14-15 0.7978798055720233 2313 16-17 0.7874596921044699 2313 18-19 0.7532786146575319 2313 20-21 0.7249138496834746 2313 22-23 0.7224759856933218 2313 24-25 0.6833799317814879 2313 26-27 0.7816149157303656 2313 28-29 0.87333609555251 2313 30-31 0.8808480879041909 2313 32-33 0.8103910727712886 2313 34-35 0.8414931269667889 2313 36-37 0.8647931972711689 2313 38-39 0.9130277088799659 2313 40-41 0.9439448658826279 2313 42-43 0.8424466409547833 2313 44-45 0.9183837578365868 2313 46-47 0.939437818322503 2313 48-49 1.0082588008574547 2313 50-51 0.9324351741271144 2313 52-53 0.7909834868821761 2313 54-55 0.7763386626260953 2313 56-57 0.770571930698857 2313 58-59 0.8104140886262883 2313 60-61 0.7679203017316922 2313 62-63 0.7688498023704469 2313 64-65 0.7734830425931989 2313 66-67 0.7436901692274418 2313 68-69 0.7906203787243626 2313 70-71 0.7580371293560759 2313 72-73 0.8308143211039578 2313 74-75 0.8163594005064283 2313 76-77 0.8480878232109035 2313 78-79 0.7902301578823518 2313 80-81 0.7784933323946994 2313 82-83 0.8144056326274267 2313 84-85 0.8335939169769482 2313 86-87 0.8376761605187646 2313 88-89 0.8208917733543863 2313 90-91 1.0022466490525836 2313 92-93 0.9544611035719726 2313 94-95 0.9277401044007014 2313 96-97 1.057270374959888 2313 98-99 1.0493242291034903 2313 100 1.0429793015822924 2314 1 0.32434572812118745 2314 2 0.23764622975765448 2314 3 0.2995061617016255 2314 4 0.18024284466412865 2314 5 0.2205294201894219 2314 6 0.21182520730075538 2314 7 0.29107012583610725 2314 8 0.33095417971806285 2314 9 0.4280676915058166 2314 10-11 0.44271091240229055 2314 12-13 0.46311384098728325 2314 14-15 0.4994433382108667 2314 16-17 0.5564075120921856 2314 18-19 0.6019328916841573 2314 20-21 0.5753916494908822 2314 22-23 0.6136162068589996 2314 24-25 0.6496347033327652 2314 26-27 0.7010694171792338 2314 28-29 0.6617340700055081 2314 30-31 0.7066400862125448 2314 32-33 0.7365787018453958 2314 34-35 0.6860843557853755 2314 36-37 0.7491878945406043 2314 38-39 0.7731867016470275 2314 40-41 0.8332111318900317 2314 42-43 0.7130223326225007 2314 44-45 0.8395492946559031 2314 46-47 0.6598077097878985 2314 48-49 0.5667876195167736 2314 50-51 0.6814052521444225 2314 52-53 0.6509759496546863 2314 54-55 0.6785423806969462 2314 56-57 0.6304796088717737 2314 58-59 0.559783536551997 2314 60-61 0.43802360890208547 2314 62-63 0.5947800752799779 2314 64-65 0.62186893022146 2314 66-67 0.6664297745950094 2314 68-69 0.6542007422942788 2314 70-71 0.6507417304540155 2314 72-73 0.7162894832501294 2314 74-75 0.7728008750032629 2314 76-77 0.7456409503498591 2314 78-79 0.6518786924699675 2314 80-81 0.7318180933290215 2314 82-83 0.7504360850894791 2314 84-85 0.8347217381877243 2314 86-87 0.7975660455299938 2314 88-89 0.6680116020095568 2314 90-91 0.7108148668556176 2314 92-93 0.889952419551264 2314 94-95 0.8523127553902263 2314 96-97 0.8169896777037167 2314 98-99 0.8503432836271898 2314 100 0.9131225604790565 2315 1 0.28726585581787845 2315 2 0.3452961323669186 2315 3 0.40243044203940315 2315 4 0.27807543968405213 2315 5 0.2656335872279243 2315 6 0.33244884118298046 2315 7 0.342458047956832 2315 8 0.3059980561192859 2315 9 0.1955686939700314 2315 10-11 0.322669719154554 2315 12-13 0.32320779300464864 2315 14-15 0.49472815703553863 2315 16-17 0.2761482932884647 2315 18-19 0.4680909490572418 2315 20-21 0.34324598340844403 2315 22-23 0.3187091415387968 2315 24-25 0.48367249831604653 2315 26-27 0.5747715086111072 2315 28-29 0.48059586558847656 2315 30-31 0.6191492035799868 2315 32-33 0.6179333433439567 2315 34-35 0.555361504717645 2315 36-37 0.6756227272272426 2315 38-39 0.7731074266577096 2315 40-41 0.7085848993349941 2315 42-43 0.7148000854130387 2315 44-45 0.6537193197264415 2315 46-47 0.7620214601463502 2315 48-49 0.6882369031564366 2315 50-51 0.6010755336316151 2315 52-53 0.2900683060583802 2315 54-55 0.4907829447422145 2315 56-57 0.48515130035314513 2315 58-59 0.4990458954187176 2315 60-61 0.5721854774239858 2315 62-63 0.5256671579484689 2315 64-65 0.5688124257457687 2315 66-67 0.38236547529073306 2315 68-69 0.4420233602667736 2315 70-71 0.3850346034986174 2315 72-73 0.508667512804081 2315 74-75 0.25651136690680687 2315 76-77 0.43398327313976637 2315 78-79 0.5094785313617685 2315 80-81 0.581244071014229 2315 82-83 0.5213813999227348 2315 84-85 0.5572368465431019 2315 86-87 0.4286603140523795 2315 88-89 0.35120839793163583 2315 90-91 0.5901655615603474 2315 92-93 0.8246783154925019 2315 94-95 0.7094606524761211 2315 96-97 0.7084364269234626 2315 98-99 0.8743768724252909 2315 100 0.9118535062222435 2316 1 0.07581570765922052 2316 2 0.1365737538791123 2316 3 0.11510765685688185 2316 4 -0.05691174398800314 2316 5 -0.07741225784771189 2316 6 -0.14704482658176943 2316 7 -0.10888565839483277 2316 8 -0.03648796725943981 2316 9 0.023742386158922102 2316 10-11 -0.017821911962734305 2316 12-13 -0.03829809317991817 2316 14-15 -0.14422611288055265 2316 16-17 -0.01479441178071994 2316 18-19 0.0649238060253623 2316 20-21 0.1503464598062081 2316 22-23 0.03967650879232565 2316 24-25 0.049918185821276495 2316 26-27 -0.0017455398272616662 2316 28-29 0.024928970972901254 2316 30-31 -0.5680118363455833 2316 32-33 -0.3660497990160323 2316 34-35 -0.4812563091847295 2316 36-37 -0.20076728099307672 2316 38-39 -0.14710108067893657 2316 40-41 0.02179569649618429 2316 42-43 0.12200926072707574 2316 44-45 -0.059811159305645845 2316 46-47 -0.15200759101080052 2316 48-49 -0.06051045612336026 2316 50-51 0.10619242723399935 2316 52-53 -0.2570186801746033 2316 54-55 -0.18120071984133546 2316 56-57 -0.16973949779438868 2316 58-59 -0.5146457002000773 2316 60-61 -0.019281074755518546 2316 62-63 0.017765896042497786 2316 64-65 -0.33687444593494575 2316 66-67 -0.6609001724337205 2316 68-69 -0.6381898358512501 2316 70-71 -0.4282471357426445 2316 72-73 -0.5939910726219537 2316 74-75 -0.3816191679853702 2316 76-77 -0.13972981445633792 2316 78-79 -0.023918116592898997 2316 80-81 -0.11091637442121893 2316 82-83 0.09679286154565148 2316 84-85 0.07380170932086472 2316 86-87 0.15189989698643913 2316 88-89 0.2372188736531271 2316 90-91 -0.3952526274445418 2316 92-93 -0.5781838614350789 2316 94-95 -0.14721854771860876 2316 96-97 0.158191007488103 2316 98-99 0.18395026290129124 2316 100 -0.004617361198647529 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 229.0 1 551.0 2 337.0 3 325.0 4 310.0 5 311.0 6 307.0 7 668.0 8 1898.0 9 3824.0 10 6246.0 11 10167.0 12 15600.0 13 20338.0 14 23590.0 15 27125.0 16 31820.0 17 38231.0 18 46197.0 19 54356.0 20 64880.0 21 77523.0 22 86761.0 23 88983.0 24 88662.0 25 91108.0 26 98649.0 27 111783.0 28 129257.0 29 153028.0 30 184904.0 31 226816.0 32 284759.0 33 367094.0 34 505083.0 35 700489.0 36 1020523.0 37 1473568.0 38 1381963.0 39 337307.0 40 332.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.25782691806211 19.110385860309062 13.458850500119551 23.17293672150928 2 15.672116580931853 20.038635201067102 41.071934505512154 23.21731371248889 3 18.952920617738688 26.094400687287617 29.559080285989847 25.39359840898385 4 12.598386712604862 16.555300892632733 38.23856850694122 32.60774388782119 5 13.19931073206476 37.77970559355734 34.52733536454675 14.493648309831148 6 30.55179109009718 36.86939828835711 18.320623586390546 14.258187035155162 7 27.375332983070383 32.579240328414734 22.29033272959627 17.755093958918618 8 25.85911810901341 35.4477823224788 20.241012900653605 18.452086667854182 9 26.70451057182199 16.11855257638988 20.72211763528388 36.45481921650425 10-11 25.059892898315827 26.231303058452426 28.06469516468838 20.644108878543367 12-13 25.563702434815827 23.998974083946862 28.265728509386793 22.17159497185051 14-15 23.016131272676198 25.386050775596768 26.204563930194162 25.39325402153287 16-17 22.03173270762972 28.241197882852376 27.028650988778054 22.69841842073985 18-19 21.816342067663243 27.345601444081037 29.261511067773206 21.576545420482514 20-21 22.804374256257116 26.594529922247627 29.11006910963697 21.491026711858286 22-23 22.72889573428842 26.33147643271267 28.92737061195163 22.01225722104728 24-25 22.267467233062554 26.413678654196925 29.243709893058913 22.07514421968161 26-27 22.13410470456206 26.687829319924646 28.97235476832481 22.205711207188493 28-29 21.95927441523987 26.867348247196652 29.148829292947276 22.024548044616203 30-31 22.547642122717125 26.61832161804492 29.034337828318215 21.799698430919744 32-33 22.418737889399893 26.808350744907518 28.78311864576435 21.98979271992824 34-35 22.531644475643148 26.835466011493903 28.90398388433915 21.7289056285238 36-37 22.441875740985587 26.696054459534473 28.761098217145868 22.100971582334072 38-39 22.377829622784898 26.8721459169864 28.833195171470233 21.916829288758464 40-41 22.39254260906898 26.531433793163544 29.069423854783206 22.006599742984264 42-43 22.247070963011154 26.668738505952312 28.671282120846154 22.41290841019038 44-45 22.139268181388346 27.00845414351854 28.682840543714534 22.169437131378587 46-47 22.18686180713288 26.80850138776459 28.349804793091906 22.65483201201063 48-49 22.43936617468796 26.859472572544107 28.297518191824732 22.403643060943192 50-51 22.410941776590622 27.002479267570813 28.12285510330045 22.463723852538116 52-53 22.253650743334198 27.28743930641021 27.95181837411203 22.50709157614356 54-55 22.191336090746706 27.103731692834486 28.10291886519923 22.602013351219583 56-57 22.421401414427194 27.101862662616842 27.788261251505087 22.688474671450873 58-59 22.18822626843949 27.109295919967387 28.142089914141206 22.56038789745192 60-61 22.396789827384335 27.266292071709795 27.9393284909076 22.397589609998263 62-63 22.317100560747086 27.166143141894285 27.980179118618125 22.536577178740504 64-65 22.1134686146338 26.968609183433962 28.00441731172873 22.913504890203512 66-67 22.19610896146307 26.96129279023312 27.8611220582269 22.981476190076904 68-69 22.022034845427044 27.067965553855434 28.023147288984855 22.886852311732667 70-71 22.157177324658083 26.80177820826643 28.037774605303454 23.003269861772033 72-73 22.218227125139535 27.129384057304918 27.798625174852692 22.853763642702855 74-75 22.31997547969903 27.309298157984628 27.64661354622789 22.724112816088454 76-77 22.218441793858908 27.126087828115743 27.63150494187629 23.02396543614906 78-79 22.13911678055534 27.09670098819343 27.716557863317725 23.047624367933512 80-81 22.161676650955553 27.378649557168988 27.5368723733145 22.922801418560955 82-83 22.47903182850815 27.135725811596096 27.762856886880012 22.62238547301575 84-85 22.502075430196204 27.264605577956512 27.417294026050758 22.816024965796526 86-87 22.1516334570794 27.471090907753947 27.64515759078707 22.732118044379586 88-89 22.31282403695496 27.106314794892523 27.607079314228443 22.973781853924073 90-91 22.528628987724204 27.03574952751655 27.60071690525662 22.83490457950263 92-93 22.55956292938318 27.188838503441904 27.47666543206587 22.774933135109045 94-95 22.286528639915396 27.027598963054178 27.57472395198142 23.11114844504901 96-97 22.391598940029063 27.025329950630383 27.478794936267875 23.104276173072673 98-99 22.277331082868464 27.112436786325844 27.397016069403097 23.21321606140259 100 22.572466967518253 26.98433723056458 27.076991360199287 23.36620444171788 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3366.0 1 2399.0 2 3156.5 3 4256.5 4 2759.5 5 2097.0 6 3062.0 7 5556.0 8 8778.0 9 10329.5 10 8888.5 11 7116.5 12 7182.5 13 7691.5 14 8369.5 15 9188.5 16 9781.0 17 10571.5 18 11428.0 19 12125.0 20 13138.0 21 14816.5 22 16817.0 23 19434.0 24 23040.0 25 27524.5 26 33770.5 27 41383.0 28 49951.0 29 61912.5 30 77942.5 31 94323.5 32 113316.0 33 134553.0 34 154850.5 35 174519.5 36 197636.5 37 222851.0 38 235575.0 39 238939.5 40 239224.5 41 237499.0 42 240513.0 43 250236.0 44 261317.0 45 277810.0 46 292404.0 47 300098.0 48 308413.5 49 312396.0 50 309017.5 51 299617.0 52 288795.0 53 276986.0 54 262823.5 55 243052.5 56 219243.0 57 191890.0 58 166039.0 59 144597.5 60 120181.5 61 96969.5 62 79156.0 63 63027.0 64 47401.5 65 34488.0 66 25729.5 67 19924.0 68 15303.0 69 11737.0 70 9215.0 71 7127.5 72 5468.5 73 4245.5 74 3339.0 75 2456.0 76 1735.0 77 1134.5 78 720.0 79 494.0 80 351.5 81 244.0 82 176.5 83 126.5 84 97.5 85 77.5 86 61.5 87 51.0 88 39.0 89 28.5 90 21.5 91 18.5 92 11.5 93 11.0 94 12.0 95 12.0 96 8.5 97 4.5 98 5.0 99 4.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025219503804973294 2 0.1104062428844511 3 0.01754792672728459 4 0.01837310476589312 5 0.020474730082974228 6 0.04620997016207786 7 0.046815960284181 8 0.039183063427052064 9 0.03416752816113458 10-11 0.03733930624703612 12-13 0.036501234801574335 14-15 0.03162752701104269 16-17 0.03769387493550073 18-19 0.03706209799969107 20-21 0.0480537273420938 22-23 0.04818266141062639 24-25 0.0662656645223212 26-27 0.05816215831504833 28-29 0.050542154864772655 30-31 0.04874997131216975 32-33 0.0437860096736653 34-35 0.04439199979576843 36-37 0.04044017059524476 38-39 0.045404132233749214 40-41 0.05462291813382892 42-43 0.0558993654123015 44-45 0.058729468216591696 46-47 0.06146931717290909 48-49 0.05954175284834698 50-51 0.05437794340361701 52-53 0.06421561283265312 54-55 0.05094185047722367 56-57 0.05522890825593207 58-59 0.05366880602668781 60-61 0.048936925711542 62-63 0.05517088792509241 64-65 0.05323043019367702 66-67 0.05140601312394096 68-69 0.05798809732252935 70-71 0.05807190446707552 72-73 0.061043834746751575 74-75 0.051032104325196476 76-77 0.05141890653079423 78-79 0.062384749059490444 80-81 0.06198505344703943 82-83 0.052779160953812986 84-85 0.057981650619102716 86-87 0.05511931429767936 88-89 0.06386749084761514 90-91 0.06918602117458421 92-93 0.0742466833644881 94-95 0.07771500980801459 96-97 0.07335703829161327 98-99 0.08241465660602726 100 0.1275157937787249 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7755902.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.14669086865296 #Duplication Level Percentage of deduplicated Percentage of total 1 73.79816402439073 42.17320866176003 2 15.142921724025058 17.307357332221386 3 5.174107882753163 8.870494310902664 4 2.1599877153104585 4.937446009877389 5 1.1400417597894452 3.257480701202127 6 0.6645314832040642 2.278546514589006 7 0.42106027612150415 1.6843541005609677 8 0.2827548198144308 1.2926801823645557 9 0.21035299733763616 1.0818879940933626 >10 0.9255661634665979 9.101922128409864 >50 0.048198592494823805 1.9025738610635636 >100 0.029597247975549405 3.1858649073661405 >500 0.0018822098605256231 0.7571653408278083 >1k 6.788250382473929E-4 0.6848129024242533 >5k 6.17113671043067E-5 0.21055567801590386 >10k+ 9.256705065646005E-5 1.2736493743209742 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31825 0.4103326731049464 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25191 0.3247978120404306 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15215 0.19617318527232552 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.8680220559774995E-5 2 0.0 0.0 0.0 0.0 3.8680220559774995E-5 3 0.0 0.0 0.0 0.0 3.8680220559774995E-5 4 0.0 0.0 0.0 1.2893406853258332E-5 5.157362741303333E-5 5 0.0 0.0 0.0 1.2893406853258332E-5 5.157362741303333E-5 6 3.8680220559774995E-5 0.0 0.0 1.2893406853258332E-5 6.446703426629166E-5 7 3.8680220559774995E-5 0.0 0.0 1.2893406853258332E-5 6.446703426629166E-5 8 3.8680220559774995E-5 0.0 0.0 1.2893406853258332E-5 9.025384797280832E-5 9 3.8680220559774995E-5 0.0 0.0 1.2893406853258332E-5 1.2893406853258332E-4 10-11 4.512692398640416E-5 0.0 0.0 2.5786813706516664E-5 1.482741788124708E-4 12-13 5.157362741303333E-5 0.0 0.0 2.5786813706516664E-5 2.3208132335864997E-4 14-15 5.157362741303333E-5 0.0 0.0 3.223351713314583E-5 3.0944176447819996E-4 16-17 6.446703426629166E-5 0.0 0.0 4.512692398640416E-5 3.739087987444916E-4 18-19 6.446703426629166E-5 0.0 0.0 1.1604066167932497E-4 3.868022055977499E-4 20-21 6.446703426629166E-5 0.0 0.0 2.062945096521333E-4 4.2548242615752493E-4 22-23 6.446703426629166E-5 0.0 0.0 4.061423158776375E-4 5.028428672770749E-4 24-25 6.446703426629166E-5 0.0 0.0 9.412187002878583E-4 5.544164946901083E-4 26-27 6.446703426629166E-5 0.0 0.0 0.0018566505868691996 6.317769358096582E-4 28-29 6.446703426629166E-5 0.0 0.0 0.005073555596757153 6.446703426629166E-4 30-31 6.446703426629166E-5 0.0 0.0 0.014511529413342253 6.640104529428041E-4 32-33 6.446703426629166E-5 0.0 0.0 0.03802910351368545 6.962439700759499E-4 34-35 6.446703426629166E-5 0.0 0.0 0.07377607401434418 7.607110043422416E-4 36-37 6.446703426629166E-5 0.0 0.0 0.1298623938260179 7.736044111954999E-4 38-39 6.446703426629166E-5 0.0 0.0 0.21490085872668324 8.251780386085332E-4 40-41 6.446703426629166E-5 0.0 0.0 0.3089904952383359 8.380714454617915E-4 42-43 6.446703426629166E-5 0.0 0.0 0.4030994718602685 8.57411555741679E-4 44-45 6.446703426629166E-5 0.0 0.0 0.5159232285297055 9.154318865813415E-4 46-47 6.446703426629166E-5 0.0 0.0 0.652174305451513 9.798989208476333E-4 48-49 6.446703426629166E-5 0.0 0.0 0.7970961984821365 0.0010701527688204416 50-51 6.446703426629166E-5 0.0 0.0 0.9480135256995254 0.001095939582526958 52-53 6.446703426629166E-5 0.0 0.0 1.1001234930508406 0.0011604066167932498 54-55 6.446703426629166E-5 0.0 0.0 1.268582300292087 0.0011926401339263956 56-57 6.446703426629166E-5 0.0 0.0 1.4633565509208344 0.0011990868373530248 58-59 6.446703426629166E-5 0.0 0.0 1.6828281223769976 0.0012119802442062833 60-61 6.446703426629166E-5 0.0 0.0 1.9087786307769232 0.0012313203544861706 62-63 6.446703426629166E-5 0.0 0.0 2.1399445222489915 0.0013151274990323499 64-65 6.446703426629166E-5 0.0 0.0 2.3723288406686933 0.0013473610161654956 66-67 6.446703426629166E-5 0.0 0.0 2.63472901024278 0.0013924879401518999 68-69 6.446703426629166E-5 0.0 0.0 2.927912188679022 0.0014182747538584164 70-71 7.091373769292083E-5 0.0 0.0 3.2366577091871456 0.0014569549744181914 72-73 1.0314725482606665E-4 0.0 0.0 3.5416963752249577 0.0016116758566572915 74-75 1.0314725482606665E-4 0.0 0.0 3.844594735725129 0.001663249484070325 76-77 1.0314725482606665E-4 0.0 1.2893406853258332E-5 4.176890063850729 0.0017212698149099872 78-79 1.0314725482606665E-4 0.0 1.2893406853258332E-5 4.543791295970475 0.0017341632217632457 80-81 1.0314725482606665E-4 0.0 1.2893406853258332E-5 4.9179708562588855 0.0017728434423230207 82-83 1.0314725482606665E-4 0.0 1.2893406853258332E-5 5.300234582644288 0.001830863773162683 84-85 1.0314725482606665E-4 0.0 1.2893406853258332E-5 5.685418665681954 0.0018630972902958288 86-87 1.0314725482606665E-4 0.0 1.2893406853258332E-5 6.086958035312978 0.0019340110279887496 88 1.0314725482606665E-4 0.0 1.2893406853258332E-5 6.401279954285137 0.001985584655401783 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14130 0.0 50.829094 1 GTATCAA 25945 0.0 41.913086 1 ATCAACG 32350 0.0 32.81296 3 TATCAAC 32905 0.0 32.68973 2 CAACGCA 32465 0.0 32.66694 5 TCAACGC 33895 0.0 31.386192 4 AACGCAG 34485 0.0 31.148176 6 GTACATG 32300 0.0 28.642923 1 TACCTGG 5905 0.0 28.275843 2 ACGCAGA 38645 0.0 27.625494 7 TACATGG 33255 0.0 27.225815 2 CGCAGAG 39355 0.0 27.04248 8 ACATGGG 33170 0.0 26.533573 3 GCAGAGT 42455 0.0 24.50486 9 CATGGGG 22910 0.0 24.017601 4 GTACCTG 7280 0.0 23.840233 1 ATGGGGG 15620 0.0 23.012968 5 GTGGTAT 6750 0.0 22.158422 1 GAGTACT 25305 0.0 21.874434 12-13 ACCTGGG 7330 0.0 21.731976 3 >>END_MODULE