FastQCFastQC Report
Mon 6 Jun 2016
ERR1211289_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211289_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7755902
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285360.36792625796457973No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240960.3106795315361128No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149030.1921504423341089No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145550.048.4260561
GTATCAA260450.040.2693441
ATCAACG322250.031.9306853
CAACGCA325200.031.6495445
TATCAAC334900.030.945162
TCAACGC335150.030.7856674
AACGCAG344350.030.2025896
TACCTGG56800.026.5962052
ACGCAGA388650.026.5547717
GTACATG320950.026.1720071
CGCAGAG398750.025.8468678
TACATGG328200.025.0871122
ACATGGG329250.024.2689743
GCAGAGT437650.023.313089
GTGGTAT74400.022.5173191
CATGGGG218950.022.2441084
GTACTAG28350.022.1805921
GTACCTG72050.021.8188691
GAGTACT253800.021.05868512-13
TGGTATC79150.020.7457562
Produced by FastQC (version 0.11.5)