##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211289_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7755902 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1079263250103 33.0 31.0 34.0 30.0 34.0 2 32.23799823669768 33.0 31.0 34.0 30.0 34.0 3 32.2997932413277 34.0 31.0 34.0 30.0 34.0 4 35.943715895327195 37.0 35.0 37.0 35.0 37.0 5 35.850718975046355 37.0 35.0 37.0 35.0 37.0 6 35.870748624724754 37.0 35.0 37.0 35.0 37.0 7 35.789147155288966 37.0 35.0 37.0 33.0 37.0 8 35.77147016555908 37.0 35.0 37.0 33.0 37.0 9 37.38610351188037 39.0 37.0 39.0 34.0 39.0 10-11 37.33777676664816 39.0 37.0 39.0 34.0 39.0 12-13 37.263214388216866 39.0 37.0 39.0 34.0 39.0 14-15 38.49089351567361 40.0 38.0 41.0 34.0 41.0 16-17 38.47386319476445 40.0 38.0 41.0 34.0 41.0 18-19 38.4897870679645 40.0 38.0 41.0 34.0 41.0 20-21 38.46395609433951 40.0 38.0 41.0 34.0 41.0 22-23 38.35406829534463 40.0 38.0 41.0 34.0 41.0 24-25 38.25950585760367 40.0 38.0 41.0 34.0 41.0 26-27 38.15636543628323 40.0 38.0 41.0 33.0 41.0 28-29 38.050498381748504 40.0 37.0 41.0 33.0 41.0 30-31 38.046343481390046 40.0 37.0 41.0 33.0 41.0 32-33 37.96608453794285 40.0 37.0 41.0 33.0 41.0 34-35 37.82723576187528 40.0 37.0 41.0 33.0 41.0 36-37 37.672128270831685 40.0 37.0 41.0 33.0 41.0 38-39 37.55436047283733 40.0 36.5 41.0 32.0 41.0 40-41 37.44385798067072 40.0 36.0 41.0 32.0 41.0 42-43 37.33213925343564 40.0 36.0 41.0 31.5 41.0 44-45 37.1671886132651 40.0 36.0 41.0 31.0 41.0 46-47 36.917858877020365 39.0 36.0 41.0 31.0 41.0 48-49 35.371789251591885 39.0 35.0 40.5 24.0 41.0 50-51 36.095935714504904 39.0 35.0 40.0 28.5 41.0 52-53 36.19063301728155 39.0 35.0 40.0 29.0 41.0 54-55 36.02839514991293 38.5 35.0 40.0 29.0 41.0 56-57 35.817199727381805 38.0 35.0 40.0 28.0 41.0 58-59 35.582533404883144 38.0 34.0 40.0 28.0 41.0 60-61 35.330964083868 37.5 34.0 40.0 28.0 41.0 62-63 35.01656235470742 37.0 34.0 40.0 27.0 41.0 64-65 34.687060576577686 36.5 34.0 39.5 26.0 41.0 66-67 34.351164442253136 36.0 33.0 39.0 26.0 40.5 68-69 33.995228536925815 35.5 33.0 39.0 26.0 40.0 70-71 33.63427245470611 35.0 32.5 38.0 26.0 40.0 72-73 33.2588771750855 35.0 32.0 37.5 25.0 39.5 74-75 32.89990280949914 35.0 32.0 37.0 25.0 39.0 76-77 31.968964344830557 34.0 30.5 36.0 24.5 38.5 78-79 32.403933546349606 35.0 31.5 36.0 25.0 38.5 80-81 32.27371613256588 35.0 32.0 36.0 25.0 37.0 82-83 32.001907063807664 34.0 31.0 35.0 24.5 37.0 84-85 31.71945190901071 34.0 31.0 35.0 24.0 36.5 86-87 31.45926237850865 34.0 31.0 35.0 23.5 36.0 88-89 31.229844833005885 34.0 31.0 35.0 23.0 36.0 90-91 31.006715788827655 34.0 30.5 35.0 21.5 35.0 92-93 30.81557857487111 34.0 30.0 35.0 20.0 35.0 94-95 30.620002741138297 34.0 30.0 35.0 20.0 35.0 96-97 30.430593179233057 34.0 29.5 35.0 20.0 35.0 98-99 30.213419857548484 34.0 29.0 35.0 19.0 35.0 100 27.788814505392153 31.0 25.0 34.0 12.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.2745003119508276 1101 2 -0.25961730365297697 1101 3 -0.29341050054244633 1101 4 -0.15753765260109986 1101 5 -0.13074363918872223 1101 6 -0.21390293679934302 1101 7 -0.21450290056055366 1101 8 -0.2314079130260609 1101 9 -0.35727543118542826 1101 10-11 -0.33217245896445746 1101 12-13 -0.3944732632204122 1101 14-15 -0.4945970121494909 1101 16-17 -0.4654914634635716 1101 18-19 -0.4998950423395385 1101 20-21 -0.5121303161337352 1101 22-23 -0.5645346694454005 1101 24-25 -0.5556716445300367 1101 26-27 -0.6448370055501371 1101 28-29 -0.7080336292258451 1101 30-31 -0.5099969587454325 1101 32-33 -0.4887425756716439 1101 34-35 -0.4949893625230928 1101 36-37 -0.516998058985024 1101 38-39 -0.5025580349141805 1101 40-41 -0.610606735164005 1101 42-43 -0.6423113939731095 1101 44-45 -0.6255581247552158 1101 46-47 -0.6061005778129811 1101 48-49 0.5715106191312103 1101 50-51 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1105 68-69 -0.616407342877558 1105 70-71 -0.7029517896809523 1105 72-73 -0.6423065438742768 1105 74-75 -0.7551654502706455 1105 76-77 -0.73488881736019 1105 78-79 -0.5661774257452237 1105 80-81 -0.5925573193597344 1105 82-83 -0.49455624925893815 1105 84-85 -0.5446769560517843 1105 86-87 -0.44965726847537724 1105 88-89 -0.5977655992606614 1105 90-91 -0.6085077991005186 1105 92-93 -0.6177798235545922 1105 94-95 -0.5417296921240755 1105 96-97 -0.6529486735635608 1105 98-99 -0.7519842442425322 1105 100 -0.9064564010825364 1106 1 -0.29589382105222484 1106 2 -0.2994122457310553 1106 3 -0.3432562300203905 1106 4 -0.2319135371880776 1106 5 -0.23241576336846492 1106 6 -0.3278454008773366 1106 7 -0.31935723917502656 1106 8 -0.3930281366178434 1106 9 -0.45443680153443466 1106 10-11 -0.4609928699188046 1106 12-13 -0.48058322018196264 1106 14-15 -0.6134263141516385 1106 16-17 -0.5778164796915419 1106 18-19 -0.6001191256580469 1106 20-21 -0.6288231644061923 1106 22-23 -0.635529266868879 1106 24-25 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0.9362337353126584 2310 96-97 1.1167720042644724 2310 98-99 1.1909386949258938 2310 100 1.3710602519382569 2311 1 0.3148400671007465 2311 2 0.3925021699805171 2311 3 0.4149478533753381 2311 4 0.2694821989558349 2311 5 0.288911004279214 2311 6 0.3486465168020203 2311 7 0.3645651820655402 2311 8 0.1806461036248237 2311 9 0.4959226793540452 2311 10-11 0.41753037875174215 2311 12-13 0.45883839474633703 2311 14-15 0.48082633516010986 2311 16-17 0.4695471671436806 2311 18-19 0.6132991344352519 2311 20-21 0.6611376718548172 2311 22-23 0.6787366050042678 2311 24-25 0.6470569554767067 2311 26-27 0.7134251537684122 2311 28-29 0.7877790706940075 2311 30-31 0.6108372608356021 2311 32-33 0.6627169372035198 2311 34-35 0.7527874532760492 2311 36-37 0.8165074071558038 2311 38-39 0.8610059176996714 2311 40-41 0.7893473807074542 2311 42-43 0.8143823566193547 2311 44-45 0.8619067093274566 2311 46-47 0.8664329899206251 2311 48-49 -6.005324559947624 2311 50-51 -0.9991937155828268 2311 52-53 0.4116263341070052 2311 54-55 0.8516109410589934 2311 56-57 0.983555212372103 2311 58-59 0.9793952135273472 2311 60-61 0.8397269351240695 2311 62-63 0.7200883620632723 2311 64-65 0.7880645952104217 2311 66-67 0.9189705550872702 2311 68-69 0.9698796160400889 2311 70-71 0.9130487772124809 2311 72-73 0.5058739340897276 2311 74-75 0.7733545936873512 2311 76-77 0.8502192249256275 2311 78-79 0.7980298260567977 2311 80-81 0.8062858538499569 2311 82-83 0.8419729853399289 2311 84-85 0.8667669278332397 2311 86-87 0.9039231068777411 2311 88-89 0.9059445440145311 2311 90-91 0.9654187454465379 2311 92-93 0.9716812634873015 2311 94-95 0.9777564775765804 2311 96-97 1.072557334239935 2311 98-99 1.0120740792730913 2311 100 1.1311390840388889 2312 1 0.04258756425754484 2312 2 0.20019920319513318 2312 3 0.22046942407518344 2312 4 -0.0028850787721097504 2312 5 0.16077052870199537 2312 6 0.08728837623339558 2312 7 0.25633796395498365 2312 8 0.3142101022274346 2312 9 0.2740428433187745 2312 10-11 0.23515480508878994 2312 12-13 0.2935100370707886 2312 14-15 0.32441857916929706 2312 16-17 0.4501921882504192 2312 18-19 0.3068263886858844 2312 20-21 0.3293817926950666 2312 22-23 0.6145114660584525 2312 24-25 0.651121855255127 2312 26-27 0.6144131270945365 2312 28-29 0.7349482071089497 2312 30-31 0.7476749738009048 2312 32-33 0.7684769843146242 2312 34-35 0.3457844380430828 2312 36-37 0.6618245257743567 2312 38-39 0.7860516135410478 2312 40-41 0.5421324955838429 2312 42-43 0.5804007883767781 2312 44-45 0.6946882138966757 2312 46-47 0.8366333907815644 2312 48-49 -4.624074298254541 2312 50-51 -0.7537334032682566 2312 52-53 0.5344164814206493 2312 54-55 0.7730169619453733 2312 56-57 0.8557951169402997 2312 58-59 0.914678355925183 2312 60-61 0.901361405082973 2312 62-63 0.7175796241577146 2312 64-65 0.8800334301549313 2312 66-67 0.8400258940279173 2312 68-69 0.7433270914367043 2312 70-71 0.8485425693239108 2312 72-73 0.9671082806140774 2312 74-75 0.6813001269512355 2312 76-77 0.7942983079403838 2312 78-79 0.5111999215901051 2312 80-81 0.7096133236722508 2312 82-83 0.636420127778365 2312 84-85 0.6833907349885919 2312 86-87 0.7590901944737709 2312 88-89 0.8366078122661094 2312 90-91 0.6310046725548055 2312 92-93 0.8411847307355913 2312 94-95 0.8963738150445586 2312 96-97 1.0144506059680722 2312 98-99 1.0364268409122488 2312 100 0.6148061579951509 2313 1 0.06882364707919209 2313 2 0.2258438476207303 2313 3 0.316075334547115 2313 4 0.11399072400354981 2313 5 0.1788758889168065 2313 6 0.29238105228232314 2313 7 0.16793969848934154 2313 8 0.24659970203631332 2313 9 0.4662966900813643 2313 10-11 0.19277840146589398 2313 12-13 -0.021495215366080345 2313 14-15 0.13090969557308796 2313 16-17 0.11173722652365825 2313 18-19 0.45154429284797715 2313 20-21 0.4008363888467912 2313 22-23 0.4765925432223881 2313 24-25 0.31798482744543577 2313 26-27 0.44753405106668254 2313 28-29 0.4169394161546123 2313 30-31 0.5865401545467677 2313 32-33 0.5083836481107582 2313 34-35 0.4643072294408057 2313 36-37 -0.209372283525461 2313 38-39 0.19838594467224624 2313 40-41 0.41309839147066185 2313 42-43 0.5905975514132606 2313 44-45 0.5631909139008968 2313 46-47 0.5198063885221842 2313 48-49 -5.29455779843925 2313 50-51 -1.283368887144924 2313 52-53 0.12964425893770937 2313 54-55 0.4010369275292476 2313 56-57 0.34683256327822676 2313 58-59 0.4389072195679944 2313 60-61 0.7376965487795175 2313 62-63 0.7872845537216122 2313 64-65 0.8956920631572558 2313 66-67 0.9783262077096779 2313 68-69 0.9960667471981068 2313 70-71 0.9304439412300596 2313 72-73 0.6830877652979339 2313 74-75 0.8249607271307653 2313 76-77 0.8319552886736155 2313 78-79 0.7885004898353927 2313 80-81 -0.0632837538857487 2313 82-83 0.3903322978068289 2313 84-85 0.7560163668754001 2313 86-87 0.8198506135862544 2313 88-89 0.8184334090880299 2313 90-91 0.8898658091636626 2313 92-93 0.9514839080343123 2313 94-95 0.780628564390117 2313 96-97 0.786785566147433 2313 98-99 1.004268885461606 2313 100 1.0214053083628265 2314 1 0.06714910058378365 2314 2 0.18467485531784433 2314 3 0.2085486798015026 2314 4 0.05350366291074948 2314 5 0.17749439542586742 2314 6 0.2723848172121919 2314 7 0.08577201985963256 2314 8 0.21740152894827247 2314 9 0.4083431099719803 2314 10-11 0.30939984845148416 2314 12-13 0.2109125235909275 2314 14-15 0.17812333434464023 2314 16-17 0.4294174218874147 2314 18-19 0.5019921426751708 2314 20-21 0.5376158671056075 2314 22-23 0.21029233095696753 2314 24-25 0.33948500832416073 2314 26-27 0.4212880692635963 2314 28-29 0.5546919003820605 2314 30-31 0.45859387589627687 2314 32-33 0.4219272833531562 2314 34-35 0.2782311704898177 2314 36-37 0.37807775379229014 2314 38-39 0.4084923315700024 2314 40-41 0.21477299322434362 2314 42-43 0.20517261903096085 2314 44-45 0.4330572552068901 2314 46-47 0.5392786831327427 2314 48-49 -6.468982651986423 2314 50-51 -1.5366833700417502 2314 52-53 -0.7949017023092964 2314 54-55 -0.33379005515575955 2314 56-57 -0.077668178287702 2314 58-59 0.4244427421601529 2314 60-61 0.637970703456979 2314 62-63 0.48269242603522855 2314 64-65 0.6676117647920918 2314 66-67 0.794021065670151 2314 68-69 0.7619017585407875 2314 70-71 0.6095901715479854 2314 72-73 0.7221950901126561 2314 74-75 0.7655183788165445 2314 76-77 0.6944501404451593 2314 78-79 0.47283151116287314 2314 80-81 0.5916958263429919 2314 82-83 0.5504147947142073 2314 84-85 0.5894280748494651 2314 86-87 0.5464533980134405 2314 88-89 0.48701136708143267 2314 90-91 0.6768963625641433 2314 92-93 0.6821593187690027 2314 94-95 0.728555366343759 2314 96-97 0.8475240155120716 2314 98-99 0.791683778522124 2314 100 -0.5444716071649687 2315 1 0.17329774609697068 2315 2 0.2504101524101614 2315 3 0.315699254091534 2315 4 0.1519811666618125 2315 5 0.24567380141970574 2315 6 0.3092984053915586 2315 7 0.30326957170517943 2315 8 0.2758428725953834 2315 9 0.4813022177609767 2315 10-11 0.2711461411186775 2315 12-13 0.4508126519777349 2315 14-15 0.28854673120505936 2315 16-17 0.3896234297714827 2315 18-19 0.4896404746580032 2315 20-21 0.40022820556608707 2315 22-23 0.417476489285626 2315 24-25 0.45976405628657346 2315 26-27 0.5969857189163363 2315 28-29 0.6527358160489385 2315 30-31 0.599304572457207 2315 32-33 0.6773472831389782 2315 34-35 0.6706100032050131 2315 36-37 0.6232965646615014 2315 38-39 0.6430905248736565 2315 40-41 0.711764261969229 2315 42-43 0.7429096557110384 2315 44-45 0.729503355948296 2315 46-47 0.6279836480970573 2315 48-49 -4.906203166747339 2315 50-51 -0.8599953630834065 2315 52-53 0.3675328103027127 2315 54-55 0.2922189533387183 2315 56-57 0.6679954649230879 2315 58-59 0.8048611951169207 2315 60-61 0.6831688106141556 2315 62-63 0.46633229924284336 2315 64-65 0.600241469383441 2315 66-67 -0.19249123935502155 2315 68-69 0.6463555613010428 2315 70-71 0.6307690575692959 2315 72-73 0.7487642887005208 2315 74-75 0.9348063258055248 2315 76-77 0.8794081202388107 2315 78-79 0.7109978226537876 2315 80-81 0.5710053813440652 2315 82-83 0.6498457401004316 2315 84-85 0.7089815112492062 2315 86-87 0.7454187775993404 2315 88-89 0.6417188141114636 2315 90-91 0.7247863687395615 2315 92-93 0.7875829774004899 2315 94-95 0.3880717264408027 2315 96-97 0.5671755863169814 2315 98-99 0.5559011008658352 2315 100 0.657070462420922 2316 1 0.17277930677146003 2316 2 0.22814889024051155 2316 3 0.15948982315895677 2316 4 -0.06966004353939326 2316 5 0.1541029388084496 2316 6 0.097968119623701 2316 7 0.24811489074861015 2316 8 0.28970853052504 2316 9 0.43357434188758504 2316 10-11 0.3932025117211282 2316 12-13 0.3327376317199011 2316 14-15 0.397686937364945 2316 16-17 0.3517582913798023 2316 18-19 0.029187275434473747 2316 20-21 0.4118988460797439 2316 22-23 0.5309801491230246 2316 24-25 0.6056020700597031 2316 26-27 0.5753170364358198 2316 28-29 0.588792727918154 2316 30-31 0.5543131028410144 2316 32-33 0.5092288602199275 2316 34-35 0.4545720088169034 2316 36-37 0.3593358920746752 2316 38-39 0.4776795405518399 2316 40-41 0.10394185850550741 2316 42-43 0.07427166430148446 2316 44-45 0.3100628883348264 2316 46-47 0.36970692356912593 2316 48-49 -5.055010298529069 2316 50-51 -1.0133965606574904 2316 52-53 0.2383120098382534 2316 54-55 0.5381951647008236 2316 56-57 0.4686807337998573 2316 58-59 0.3188574821782453 2316 60-61 0.5216294950419567 2316 62-63 0.4064194939746102 2316 64-65 0.4436005326232717 2316 66-67 0.47745568622774925 2316 68-69 0.15672517790008555 2316 70-71 -0.26772718762137515 2316 72-73 -0.07367699288833052 2316 74-75 0.11002364429133848 2316 76-77 0.21716516397008334 2316 78-79 0.24986869915682774 2316 80-81 0.2082953902235758 2316 82-83 0.08115395771164913 2316 84-85 0.21440445739573022 2316 86-87 0.24798897076834692 2316 88-89 0.09141426915519091 2316 90-91 0.11880721422413743 2316 92-93 0.2555126222420867 2316 94-95 0.49831114726338654 2316 96-97 0.5406471729854623 2316 98-99 0.6220638588312077 2316 100 -0.1268127152666807 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 16.0 11 15.0 12 34.0 13 181.0 14 719.0 15 1988.0 16 4936.0 17 10076.0 18 17218.0 19 25445.0 20 35922.0 21 49402.0 22 62021.0 23 66088.0 24 67234.0 25 73067.0 26 84362.0 27 102664.0 28 126542.0 29 154548.0 30 190768.0 31 235531.0 32 295516.0 33 384613.0 34 532474.0 35 750862.0 36 1153910.0 37 1716118.0 38 1419337.0 39 194270.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.462216934933245 19.881239774112842 14.259146002518056 23.397397288435858 2 15.476691947255153 20.46870239420982 40.6173069204241 23.437298738110922 3 18.625445548192697 25.978897341957314 30.215996993254805 25.17966011659518 4 12.645275940623874 16.977958666777575 38.13257040130663 32.24419499129193 5 13.092484110728117 37.92625500556869 34.609653962383554 14.371606921319641 6 30.69598130351895 37.18144565593823 17.996781288253146 14.125791752289677 7 27.376848592684276 32.62166866514524 22.14906071506853 17.852422027101948 8 25.973206621870492 35.78938654822797 19.936837677886597 18.300569152014944 9 26.494716293231253 16.08487715193176 20.57739797734823 36.84300857748875 10-11 25.091097805677997 26.43657257057207 27.90682472016955 20.56550490358038 12-13 25.500445209797867 24.013311682702213 28.322956708794628 22.163286398705296 14-15 22.87801637399153 25.495637352092395 26.155773719289094 25.47057255462698 16-17 21.999186938723724 28.372460456740328 26.981907774921016 22.646444829614925 18-19 21.761789203173663 27.398453267735096 29.269751367974788 21.57000616111645 20-21 22.69218857700295 26.600846220515017 29.178929377359008 21.528035825123023 22-23 22.648106378156026 26.356712934370552 28.9954212791898 21.999759408283627 24-25 22.26629978888325 26.45105698914555 29.246897006639333 22.035746215331866 26-27 22.096012130128116 26.691503688484698 29.053655316375483 22.15882886501171 28-29 21.927581255555907 26.90380331630806 29.20589283652471 21.96272259161132 30-31 22.50989625580427 26.605052715347437 29.11047098067476 21.774580048173533 32-33 22.40582110416534 26.789153700355158 28.832483297251986 21.972541898227515 34-35 22.489996558753024 26.830655378620023 28.993463307196322 21.685884755430635 36-37 22.404034840464135 26.696578828706986 28.856520729026414 22.04286560180247 38-39 22.31289091213907 26.819590420798782 28.970261661271813 21.897257005790337 40-41 22.303446277743205 26.50515523003756 29.204292579121034 21.987105913098205 42-43 22.196376086864163 26.626683488252194 28.816352087157632 22.360588337726014 44-45 22.07682930351692 26.984941185208566 28.761879783247146 22.176349728027365 46-47 22.144511428775722 26.797795141421986 28.43141319344531 22.626280236356983 48-49 22.514675628962518 26.900406683095802 28.29012366219661 22.29479402574507 50-51 22.363547746658348 26.969837513402673 28.18641239592436 22.480202344014614 52-53 22.208622834368054 27.198529254359507 28.037674961189946 22.555172950082493 54-55 22.15594358217739 27.075411166119807 28.141207803136446 22.627437448566358 56-57 22.389797750160568 27.05114006418055 27.858728775346027 22.700333410312858 58-59 22.13369482426767 27.05694784552839 28.186228778837258 22.623128551366687 60-61 22.306781392753237 27.296971600600138 27.98529459548533 22.410952411161293 62-63 22.28075464379967 27.140979358612345 27.995222905092117 22.58304309249587 64-65 22.02772173090399 26.937301065121293 28.03350140087811 23.001475803096607 66-67 22.100954020608466 26.9579192841676 27.889305416275427 23.051821278948513 68-69 21.91581807291879 27.019142820146275 28.039248030466318 23.025791076468618 70-71 22.03041047656252 26.749736034050738 28.073976497583587 23.14587699180316 72-73 22.04937077656057 27.08453175514467 27.841413111723874 23.02468435657089 74-75 22.132779293793007 27.22928114014374 27.679478025121973 22.95846154094128 76-77 21.943481864543326 27.06178489554974 27.72763335586786 23.267099884039077 78-79 21.889238121463006 26.994329073621465 27.818698757752564 23.297734047162965 80-81 21.847690660666093 27.249270633493833 27.636733897623465 23.266304808216614 82-83 22.16743883517581 26.99558749798834 27.860917854062734 22.976055812773122 84-85 22.148659662987715 27.117662838114935 27.513915362611908 23.21976213628544 86-87 21.764229168880558 27.324572173175593 27.763831700298887 23.147366957644962 88-89 21.84876973467794 26.94872699852458 27.781949788792343 23.420553478005132 90-91 22.023181991399603 26.85155774960844 27.76981015037473 23.355450108617223 92-93 22.002317645330116 26.975654665816705 27.652521095337264 23.36950659351592 94-95 21.627251887785796 26.795275848436606 27.821434198857 23.756038064920592 96-97 21.687391526262594 26.764695775831765 27.81797048760051 23.729942210305133 98-99 21.486771874754584 26.86733523952552 27.729654441735992 23.916238443983907 100 21.48275452901588 26.814237477009904 27.711631374349636 23.991376619624575 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3240.0 1 2296.0 2 2688.0 3 3619.0 4 2455.5 5 1843.5 6 2743.5 7 5145.0 8 8172.0 9 9601.5 10 8215.0 11 6487.0 12 6453.5 13 7019.5 14 7778.0 15 8507.5 16 9423.0 17 10531.0 18 11343.0 19 12138.0 20 13338.0 21 14757.0 22 16676.5 23 19509.0 24 23050.0 25 27581.0 26 33758.0 27 41552.5 28 50470.5 29 62477.5 30 78533.0 31 94819.0 32 114035.0 33 135158.5 34 155705.5 35 175631.5 36 198008.0 37 223270.5 38 236702.5 39 239718.5 40 238947.5 41 237674.5 42 241575.0 43 252099.0 44 264343.0 45 280493.5 46 294419.0 47 302285.5 48 311550.0 49 316070.0 50 312195.5 51 302556.0 52 291039.0 53 278372.5 54 265194.0 55 245016.5 56 219413.5 57 191635.5 58 165290.5 59 143237.0 60 118431.5 61 94649.0 62 75920.0 63 59615.0 64 44328.0 65 31944.0 66 23521.5 67 17838.0 68 13497.5 69 10093.0 70 7792.5 71 5975.0 72 4587.5 73 3543.5 74 2662.0 75 1938.5 76 1307.0 77 776.5 78 476.5 79 287.0 80 169.5 81 103.5 82 64.5 83 49.0 84 31.5 85 16.5 86 12.0 87 10.5 88 9.0 89 5.5 90 2.5 91 1.5 92 1.0 93 0.5 94 0.0 95 1.0 96 1.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02960326213508113 2 0.0019211176211354915 3 9.025384797280832E-5 4 0.02071970481318614 5 5.157362741303333E-4 6 5.157362741303333E-5 7 2.5786813706516664E-5 8 0.004241930854721992 9 0.010765994722470707 10-11 0.0011539599133666206 12-13 1.0314725482606665E-4 14-15 5.8020330839662486E-5 16-17 3.739087987444916E-4 18-19 3.223351713314583E-5 20-21 5.157362741303333E-5 22-23 3.0944176447819996E-4 24-25 5.157362741303333E-5 26-27 9.025384797280832E-5 28-29 0.001012132437980779 30-31 0.0024626407089723412 32-33 0.009554014478264422 34-35 0.018637419606384917 36-37 0.02117742075647681 38-39 0.02065523777891985 40-41 0.0042741643718551365 42-43 0.005034875376197378 44-45 0.009154318865813416 46-47 0.012087568924929687 48-49 1.195051201007955 50-51 0.01786381519518942 52-53 0.01892752126058323 54-55 0.009850562835889365 56-57 0.0076457902639821915 58-59 0.007903658401047358 60-61 0.0049768550453577155 62-63 0.0072332012446779245 64-65 0.019082242142822333 66-67 0.02892635827528507 68-69 0.02952590169396158 70-71 0.02657331152456542 72-73 0.02295671090222646 74-75 0.009534674367984535 76-77 0.014627570075021577 78-79 0.008296907310071737 80-81 0.002565787963798408 82-83 0.014414828861942813 84-85 0.024265391697832178 86-87 0.023594934541462744 88-89 0.03240757812566481 90-91 0.02071970481318614 92-93 0.01351873708564136 94-95 0.006704571563694332 96-97 0.0036166006223389622 98-99 0.015149753052578539 100 0.03303290835804785 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7755902.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.79830736698119 #Duplication Level Percentage of deduplicated Percentage of total 1 72.93896448482975 41.428097238386854 2 15.755709314356928 17.897952408433067 3 5.284518591223376 9.004551336924953 4 2.2459021050341326 5.10253752311515 5 1.1934426513026344 3.3892761266775993 6 0.6740383709223088 2.2970543141270743 7 0.41068605141428205 1.6328390805692168 8 0.2775996890158692 1.2613753969361383 9 0.2030476399529796 1.037948603777052 >10 0.9329777109774756 9.092734990399526 >50 0.05081164301890979 1.99004233189648 >100 0.029392830933987223 3.0964047106992902 >500 0.002158235255822831 0.8280803403274171 >1k 6.25568134494937E-4 0.6408409248183543 >5k 3.1278406717660406E-5 0.10046449959443553 >10k+ 9.383522015298121E-5 1.1998001733174541 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28536 0.36792625796457973 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24096 0.3106795315361128 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14903 0.1921504423341089 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.5786813706516664E-5 2 0.0 0.0 0.0 0.0 2.5786813706516664E-5 3 0.0 0.0 0.0 0.0 2.5786813706516664E-5 4 0.0 0.0 0.0 0.0 2.5786813706516664E-5 5 1.2893406853258332E-5 0.0 0.0 0.0 3.8680220559774995E-5 6 1.2893406853258332E-5 0.0 0.0 0.0 7.736044111954999E-5 7 1.2893406853258332E-5 0.0 0.0 0.0 9.025384797280832E-5 8 1.2893406853258332E-5 0.0 0.0 0.0 1.0314725482606665E-4 9 1.2893406853258332E-5 0.0 0.0 0.0 1.4182747538584164E-4 10-11 1.2893406853258332E-5 0.0 0.0 2.5786813706516664E-5 1.5472088223909998E-4 12-13 1.2893406853258332E-5 0.0 0.0 3.8680220559774995E-5 2.256346199320208E-4 14-15 1.2893406853258332E-5 0.0 0.0 3.8680220559774995E-5 2.836549507716833E-4 16-17 2.5786813706516664E-5 0.0 0.0 4.512692398640416E-5 3.352285781847166E-4 18-19 2.5786813706516664E-5 0.0 0.0 1.2248736510595416E-4 3.4812198503797497E-4 20-21 2.5786813706516664E-5 0.0 0.0 1.8050769594561664E-4 4.3837583301078325E-4 22-23 2.5786813706516664E-5 0.0 0.0 3.803555021711208E-4 4.7705605357055826E-4 24-25 2.5786813706516664E-5 0.0 0.0 9.154318865813415E-4 5.286296809835916E-4 26-27 2.5786813706516664E-5 0.0 0.0 0.0018373104765893123 5.673099015433665E-4 28-29 2.5786813706516664E-5 0.0 0.0 0.005028428672770749 5.673099015433665E-4 30-31 2.5786813706516664E-5 0.0 0.0 0.014505082709915622 5.930967152498832E-4 32-33 2.5786813706516664E-5 0.0 0.0 0.03810001725137837 6.446703426629166E-4 34-35 2.5786813706516664E-5 0.0 0.0 0.0738469877520371 7.091373769292082E-4 36-37 2.5786813706516664E-5 0.0 0.0 0.12918548996622187 7.155840803558374E-4 38-39 2.5786813706516664E-5 0.0 0.0 0.21051065369314878 7.478175974889832E-4 40-41 2.5786813706516664E-5 0.0 0.0 0.30001668406846815 7.736044111954999E-4 42-43 3.223351713314583E-5 0.0 0.0 0.3897419023602928 7.80051114622129E-4 44-45 3.8680220559774995E-5 0.0 0.0 0.49836240839556767 8.316247420351624E-4 46-47 3.8680220559774995E-5 0.0 0.0 0.628095868153053 8.831983694481957E-4 48-49 3.8680220559774995E-5 0.0 0.0 0.7676283171190146 9.283252934345999E-4 50-51 3.8680220559774995E-5 0.0 0.0 0.9179009739937405 9.283252934345999E-4 52-53 3.8680220559774995E-5 0.0 0.0 1.0694887583674988 9.73452217421004E-4 54-55 3.8680220559774995E-5 0.0 0.0 1.237489849665455 0.001012132437980779 56-57 3.8680220559774995E-5 0.0 0.0 1.4319224250125906 0.0010314725482606665 58-59 3.8680220559774995E-5 0.0 0.0 1.651374656358474 0.001089492879100329 60-61 3.8680220559774995E-5 0.0 0.0 1.8770544032144811 0.001166853320219879 62-63 3.8680220559774995E-5 0.0 0.0 2.108342782051656 0.0012377670579127999 64-65 3.8680220559774995E-5 0.0 0.0 2.3403725317828927 0.0012506604647660581 66-67 4.512692398640416E-5 0.0 0.0 2.6020764573869037 0.0012635538716193164 68-69 5.157362741303333E-5 6.446703426629166E-6 0.0 2.8944989248188024 0.0012764472784725749 70-71 5.157362741303333E-5 1.2893406853258332E-5 0.0 3.2026449019082497 0.0013151274990323499 72-73 5.157362741303333E-5 1.2893406853258332E-5 0.0 3.5072774256301846 0.0014827417881247082 74-75 5.157362741303333E-5 1.2893406853258332E-5 0.0 3.810066192172103 0.0015665489326708872 76-77 5.157362741303333E-5 1.2893406853258332E-5 0.0 4.142832129647847 0.001592335746377404 78-79 5.157362741303333E-5 1.2893406853258332E-5 0.0 4.5096044276990614 0.001631015966937179 80-81 5.157362741303333E-5 1.2893406853258332E-5 0.0 4.88326825171334 0.0016568027806436957 82-83 5.157362741303333E-5 1.2893406853258332E-5 0.0 5.265435277547343 0.0018050769594561665 84-85 5.157362741303333E-5 1.2893406853258332E-5 0.0 5.650696721026129 0.0018115236628827957 86-87 5.157362741303333E-5 1.2893406853258332E-5 0.0 6.05226187747086 0.0018502038834425705 88 5.157362741303333E-5 1.2893406853258332E-5 0.0 6.366996385462323 0.0018824374005757163 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14555 0.0 48.426056 1 GTATCAA 26045 0.0 40.269344 1 ATCAACG 32225 0.0 31.930685 3 CAACGCA 32520 0.0 31.649544 5 TATCAAC 33490 0.0 30.94516 2 TCAACGC 33515 0.0 30.785667 4 AACGCAG 34435 0.0 30.202589 6 TACCTGG 5680 0.0 26.596205 2 ACGCAGA 38865 0.0 26.554771 7 GTACATG 32095 0.0 26.172007 1 CGCAGAG 39875 0.0 25.846867 8 TACATGG 32820 0.0 25.087112 2 ACATGGG 32925 0.0 24.268974 3 GCAGAGT 43765 0.0 23.31308 9 GTGGTAT 7440 0.0 22.517319 1 CATGGGG 21895 0.0 22.244108 4 GTACTAG 2835 0.0 22.180592 1 GTACCTG 7205 0.0 21.818869 1 GAGTACT 25380 0.0 21.058685 12-13 TGGTATC 7915 0.0 20.745756 2 >>END_MODULE