FastQCFastQC Report
Mon 6 Jun 2016
ERR1211288_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211288_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6578689
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246610.3748619215773842No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196980.2994213588756058No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119470.18160153185535902No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113950.052.132721
GTATCAA200750.040.5567251
CAACGCA249650.031.9784135
ATCAACG251950.031.70613
TATCAAC256200.031.3755172
TCAACGC262500.030.503194
AACGCAG266400.030.1448296
GTGGTAT50650.029.3446521
ACGCAGA297850.026.6782657
GTACATG260600.026.6760431
CGCAGAG304050.026.040748
TACATGG263600.025.9802862
TGGTATC55550.025.0631472
ACATGGG269600.024.3840663
GCAGAGT330250.023.5915579
GTACTAG24500.022.8455811
CATGGGG188550.022.8283184
TACCTGG47750.022.3604472
GAGTACT196950.021.81822812-13
ATGGGGG122250.021.6398665
Produced by FastQC (version 0.11.5)